Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,525 +G intergenic (+92/‑141) b4555 → / → yigM hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,5241.G99.8% 1914.0 / NA 477intergenic (+91/‑142)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  major base G (213/262);  minor base C (0/1);  total (214/263)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 539 total aligned reads displayed.

TGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTC  >  NC_000913/4008431‑4008614
                                                                                                 |                                                                                           
tgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt                                                                                              >  1:12160187/1‑96 (MQ=255)
tgAAGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt                                                                                              <  1:3719824/96‑1 (MQ=255)
 gACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑g                                                                                            <  2:11019493/96‑1 (MQ=255)
 gACGCGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑g                                                                                            <  1:2012447/96‑1 (MQ=255)
  aCGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg                                                                                           <  1:8733893/96‑2 (MQ=255)
   cGGGATGACCGACCCCGAAATGCTGTCGATGGC‑GGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                                                                         <  2:6589661/96‑4 (MQ=255)
   cGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGGGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga                                                                                          >  2:6209053/1‑94 (MQ=255)
   cGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga                                                                                          <  2:4637065/96‑3 (MQ=255)
    gggATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                                                                         >  1:5482252/1‑93 (MQ=255)
    gggATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                                                                         <  1:9130859/96‑4 (MQ=255)
    gggATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                                                                         >  2:2391646/1‑93 (MQ=255)
     ggATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                                                                                        >  2:9374012/1‑92 (MQ=255)
         gAACGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGAt                                                                                    >  1:10443163/1‑96 (MQ=255)
          aaCGACGCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGAtt                                                                                   >  2:13336172/1‑96 (MQ=255)
           acgacgCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTg                                                                                  <  1:5192299/96‑1 (MQ=255)
           acgacgCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTg                                                                                  >  1:6760282/1‑96 (MQ=255)
           acgacgCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTg                                                                                  >  1:12280360/1‑96 (MQ=255)
             gacgCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGt                                                                                >  2:6507244/1‑96 (MQ=255)
               cgCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTAc                                                                              >  1:5771707/1‑96 (MQ=255)
                gCCGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACt                                                                             <  2:3237656/96‑1 (MQ=255)
                  cGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTaa                                                                           <  2:11687205/96‑1 (MQ=255)
                  cGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTaa                                                                           <  1:4485362/96‑1 (MQ=255)
                  cGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGAACTaa                                                                           >  1:11624073/1‑96 (MQ=255)
                  cGAAATGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAACGTCTGAAAGACCTTCATCCCCAGCTGG‑AAGTGATTGGTACTaa                                                                           >  1:1192458/1‑96 (MQ=255)
                    aaaTGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCCCCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATg                                                                         <  2:2593835/96‑1 (MQ=255)
                    aaaTGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATg                                                                         >  2:13125727/1‑96 (MQ=255)
                    aaaTGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATg                                                                         >  2:9593158/1‑96 (MQ=255)
                     aaTGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGc                                                                        >  2:1425508/1‑96 (MQ=255)
                      aTGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGcc                                                                       <  1:9529456/96‑1 (MQ=255)
                       tGCTGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcg                                                                      <  2:8611170/96‑1 (MQ=255)
                          tGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcgacg                                                                   <  2:9733551/96‑1 (MQ=255)
                          tGTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGGGATTGGGACTAATGCcgacg                                                                   >  2:4132733/1‑96 (MQ=255)
                           gTCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCcgacga                                                                  >  1:13500561/1‑96 (MQ=255)
                            tCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCgacgac                                                                 <  2:6029483/96‑1 (MQ=255)
                            tCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCgacgac                                                                 <  1:215147/96‑1 (MQ=255)
                            tCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCgacgac                                                                 <  1:750949/96‑1 (MQ=255)
                            tCGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCgacgac                                                                 >  1:4409288/1‑96 (MQ=255)
                             cGATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGacgacg                                                                <  2:4841377/96‑1 (MQ=255)
                              gATGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGAcgc                                                               >  2:9173504/1‑96 (MQ=255)
                               aTGGCGGGGAAAGG‑CTGTATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGAcgcg                                                              >  2:10649372/1‑96 (MQ=255)
                               aTGGCGGGGAAAGG‑CTGCATTATTGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGGGCTGG‑AAGTGATTGGTACTAATGCCGACGAcgcg                                                              >  2:12291817/1‑96 (MQ=255)
                               aTGGCGGGGAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGAcgcg                                                              >  2:13460319/1‑96 (MQ=255)
                                 ggcggggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGt                                                            <  2:7680244/92‑1 (MQ=255)
                                   cggggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGc                                                          >  1:476229/3‑96 (MQ=255)
                                     gggAAA‑GACTGCATTATGGAGAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGATCTGG‑AAGTGATTGGTACTAATGCCGACGACTCGGTGCCg                                                        >  1:6505205/1‑96 (MQ=255)
                                      ggAAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCTTCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGATGCCGc                                                       >  2:12783958/1‑96 (MQ=255)
                                        aaagg‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAt                                                     >  2:5036623/5‑96 (MQ=255)
                                         aagg‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt                                                    <  2:12507111/93‑1 (MQ=255)
                                          agg‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTa                                                   <  2:5414297/94‑1 (MQ=255)
                                            g‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc                                                 <  2:4964563/96‑1 (MQ=255)
                                              cTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt                                                >  1:5270661/1‑96 (MQ=255)
                                              cTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt                                                >  1:14240792/1‑96 (MQ=255)
                                              cTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt                                                <  2:7571501/96‑1 (MQ=255)
                                               tGCATTATGGGCAGTGCGCCCCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg                                               <  1:4961560/96‑1 (MQ=255)
                                               tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg                                               <  2:11461145/96‑1 (MQ=255)
                                               tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg                                               >  2:10354442/1‑96 (MQ=255)
                                                gCATTATGGGCAGTGCGCAACAGCGTCTGAAAGAGCTTCATCCCGAGCT‑GTAAGTGATTGGTAGTAATGCCGACGACGCGATGCCACTCTATCTGc                                              >  1:2594343/1‑96 (MQ=255)
                                                 cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg                                             <  1:7698105/96‑1 (MQ=255)
                                                 cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg                                             <  2:4267876/96‑1 (MQ=255)
                                                   ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTa                                           >  1:98094/1‑96 (MQ=255)
                                                   ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTa                                           <  2:281241/96‑1 (MQ=255)
                                                     aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATTTGCGTaaa                                         <  2:12971223/96‑1 (MQ=255)
                                                     aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa                                         >  2:11164189/1‑96 (MQ=255)
                                                     aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa                                         >  2:10025707/1‑96 (MQ=255)
                                                     aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa                                         <  2:9287501/96‑1 (MQ=255)
                                                       gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGCCGACGCGGTGCGGCATTATGTGCGTaagtg                                       >  1:10237775/1‑93 (MQ=255)
                                                       gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAct                                       >  2:1678771/1‑96 (MQ=255)
                                                        ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAActc                                      >  2:682961/1‑96 (MQ=255)
                                                         gCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAActct                                     >  2:335932/1‑96 (MQ=255)
                                                          cAGTGCGCACCAGCGTCTGAAAGACCTTCATCCGGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTa                                    >  2:14079955/1‑96 (MQ=255)
                                                          cAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTa                                    <  1:4221818/96‑1 (MQ=255)
                                                             tgcgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAttt                                 <  2:5253056/92‑1 (MQ=255)
                                                               cgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAt                               <  2:7053275/94‑1 (MQ=255)
                                                                 caccaGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTACTGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCg                             <  2:444129/96‑1 (MQ=255)
                                                                  accaGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGt                            <  1:3025377/96‑1 (MQ=255)
                                                                   ccaGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTa                           >  1:1622001/1‑96 (MQ=255)
                                                                     aGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAt                         <  1:1000756/96‑1 (MQ=255)
                                                                     aGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAt                         >  1:12497472/1‑96 (MQ=255)
                                                                     aGCGTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAt                         >  1:13300664/1‑96 (MQ=255)
                                                                     aGCGTCTGAAAGAACTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAt                         >  1:11417905/1‑96 (MQ=255)
                                                                       cGTCTGAAAGACCTTCTTCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCg                       <  1:12812156/96‑1 (MQ=255)
                                                                        gTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGt                      >  1:6184620/1‑96 (MQ=255)
                                                                        gTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGt                      >  2:5677443/1‑96 (MQ=255)
                                                                        gTCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGt                      <  1:3444052/96‑1 (MQ=255)
                                                                         tCTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGtt                     >  1:7170957/1‑96 (MQ=255)
                                                                          cTGAAAGTCCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTc                    <  2:1208284/96‑1 (MQ=255)
                                                                          cTGAAAGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTc                    <  1:1913585/96‑1 (MQ=255)
                                                                              aaGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTa                <  1:11053208/96‑1 (MQ=255)
                                                                              aaGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTa                <  2:8374205/96‑1 (MQ=255)
                                                                               aGACCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAt               <  2:2051768/96‑1 (MQ=255)
                                                                                 aCCTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAttt             >  2:7251045/1‑96 (MQ=255)
                                                                                  ccTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTg            >  1:13907235/1‑96 (MQ=255)
                                                                                   cTTCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTgg           >  1:8329023/1‑96 (MQ=255)
                                                                                    ttCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGt          >  1:6988356/1‑96 (MQ=255)
                                                                                     tCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTc         <  1:638430/96‑1 (MQ=255)
                                                                                     tCATCCCGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTc         <  1:2782277/96‑1 (MQ=255)
                                                                                      cATCCCGAGCTGG‑AAGTGATTGGGACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCa        >  2:7915859/1‑96 (MQ=255)
                                                                                         cccGAGCTGG‑ACGTGATTGGTACTAATGCCGACGACGCGGTGACGCAATCTCTGCGTAAACTCTATTTATCGTAATCGTTCTTTACTTGGTCAGtt     <  1:3958699/96‑1 (MQ=255)
                                                                                         cccGAGCTGG‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGtt     <  2:3651263/96‑1 (MQ=255)
                                                                                           cGAGCTGG‑AAGTGATTGGTACTAATGCCTACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGt   >  2:13871094/1‑96 (MQ=255)
                                                                                                 |                                                                                           
TGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGAAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G‑AAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTC  >  NC_000913/4008431‑4008614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 306 total aligned reads displayed.
GTTGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑G‑‑AAA‑‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACT  >  NC_000913/4008427‑4008625
                                                                                                           |                                                                                                    
ttgGTGACGCGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑G                                                                                                      <  SRR4302518.2105175/98‑1 (MQ=60)
ctgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGN‑N‑N                                                                                                      >  SRR4302518.9299252/4‑101 (MQ=60)
 NNNTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑GG                                                                                                     <  SRR4302518.9158314/101‑1 (MQ=60)
  NNNGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑GGA                                                                                                    <  SRR4302518.9556257/101‑1 (MQ=60)
  GGTGACGGGATGATCGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGAAGTGCGCACCATCGTCTGAAAGACCTTCATCCCGAGC‑T‑GGA                                                                                                    <  SRR4302518.5008240/101‑1 (MQ=60)
   gacgctggtgcgcactgcccatAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑GNNN                                                                                                   >  SRR4302518.8869368/23‑101 (MQ=60)
   GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑Ggaa                                                                                                   <  SRR4302518.2816257/101‑4 (MQ=60)
    TGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGTG‑‑AAAG‑G‑CTGCATTATGGGCAGTGCGCACCATCGTCTGCAAGACCTTCATCCCGAGC‑TGGAAG                                                                                                   >  SRR4302518.7331645/1‑101 (MQ=60)
    TGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑Ggnnn                                                                                                  >  SRR4302518.7403178/1‑97 (MQ=60)
    TGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑Ggnnn                                                                                                  >  SRR4302518.12737407/1‑97 (MQ=60)
    NNNCGGCATGAACGACGCCGAAATGCTGTCGATCGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCCCCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAG                                                                                                   <  SRR4302518.10362605/101‑1 (MQ=60)
     NNNGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGCGCAGTGCGCCCCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGT                                                                                                  <  SRR4302518.7464921/101‑1 (MQ=60)
     GACGGGCTGAACGACGCCGAAATGCTGTCGATGGC‑GG‑G‑‑GAAGGG‑CTGCATTATGGGCAGTGCGCACCCGCGTCTGAAAGACCTTCATCCCGCGC‑TGGAAGT                                                                                                  <  SRR4302518.6113427/101‑1 (MQ=60)
     GACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGT                                                                                                  <  SRR4302518.87649/101‑1 (MQ=60)
     GACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGT                                                                                                  <  SRR4302518.1536043/101‑1 (MQ=60)
     GACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑Ggannn                                                                                                 >  SRR4302518.12920478/1‑96 (MQ=60)
      ACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑Ggaannn                                                                                                >  SRR4302518.13019759/1‑95 (MQ=60)
       CGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGA                                                                                                >  SRR4302518.2877628/1‑101 (MQ=60)
       CGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑Ggaagnnn                                                                                               >  SRR4302518.9107527/1‑94 (MQ=60)
        GGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGGCCTTCATCCCGAGC‑TGGAAGTGAT                                                                                               <  SRR4302518.3821173/101‑1 (MQ=60)
        GGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGAT                                                                                               >  SRR4302518.6326211/1‑101 (MQ=60)
        GGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑Ggaagtnnn                                                                                              >  SRR4302518.9552768/1‑93 (MQ=60)
          nnntAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTG                                                                                             <  SRR4302518.13949968/97‑1 (MQ=60)
          NNNGAACGACGCCGACATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTG                                                                                             <  SRR4302518.10936004/101‑1 (MQ=60)
          GATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTG                                                                                             <  SRR4302518.1398481/101‑1 (MQ=60)
           NNNAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGA                                                                                            <  SRR4302518.14424378/101‑1 (MQ=60)
            TGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTNNN                                                                                           >  SRR4302518.12916847/1‑101 (MQ=60)
            TGAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGT                                                                                           <  SRR4302518.544892/101‑1 (MQ=60)
             GAACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTA                                                                                          <  SRR4302518.2014240/101‑1 (MQ=60)
               ACGACGCCGAAATGCTGTCGATGGCTGG‑G‑‑AAAG‑G‑CTGCATTATGGGCCGTGCGCCCCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACT                                                                                        <  SRR4302518.4459599/101‑1 (MQ=60)
               ACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACT                                                                                        <  SRR4302518.2091570/101‑1 (MQ=60)
               ACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACT                                                                                        >  SRR4302518.7113379/1‑101 (MQ=60)
               ACGACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCACCCCGGGC‑TGGAAGTGATTGGTACT                                                                                        <  SRR4302518.4790671/101‑1 (MQ=60)
                 GGCGCCGAGATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACNNN                                                                                      >  SRR4302518.13572444/1‑101 (MQ=60)
                 GACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAA                                                                                      <  SRR4302518.3632379/101‑1 (MQ=60)
                  ACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAAT                                                                                     >  SRR4302518.5469194/1‑101 (MQ=60)
                  ACGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAAT                                                                                     <  SRR4302518.4576926/101‑1 (MQ=60)
                   NNNCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATG                                                                                    <  SRR4302518.12233391/101‑1 (MQ=60)
                   NNNCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATG                                                                                    <  SRR4302518.7523475/101‑1 (MQ=60)
                   CGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATG                                                                                    <  SRR4302518.2597962/101‑1 (MQ=60)
                   CGCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATG                                                                                    <  SRR4302518.6818937/101‑1 (MQ=60)
                    GCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAANNN                                                                                   >  SRR4302518.13347721/1‑101 (MQ=60)
                    GCCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGGGATTGGTACTAATGC                                                                                   >  SRR4302518.3018926/1‑101 (MQ=60)
                     NNNAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCC                                                                                  <  SRR4302518.8365975/101‑1 (MQ=60)
                     CCGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCC                                                                                  <  SRR4302518.3258024/101‑1 (MQ=60)
                      CGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAGGTGATTGGTACTAATGCCG                                                                                 >  SRR4302518.5722797/1‑101 (MQ=60)
                      CGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGAACTAATGNNN                                                                                 >  SRR4302518.12171454/1‑101 (MQ=60)
                      CGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAACGTCTGAAAGACCTTCATCCCCAGC‑TGGAAGTGATTGGTACTAATGCCG                                                                                 >  SRR4302518.1240502/1‑101 (MQ=60)
                      CGAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCAATATGGGAAGTGCGCACAAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTAATAATGaca                                                                                 >  SRR4302518.102991/1‑98 (MQ=42)
                      CGAAATGCTGTCGAGGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCG                                                                                 >  SRR4302518.5378768/1‑101 (MQ=60)
                       GAAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGA                                                                                <  SRR4302518.4130542/101‑1 (MQ=60)
                        NNNTGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGTCCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGAC                                                                               <  SRR4302518.14708944/101‑1 (MQ=60)
                        NNNTGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGAC                                                                               <  SRR4302518.10006030/101‑1 (MQ=60)
                        CAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGAC                                                                               <  SRR4302518.1989113/101‑1 (MQ=60)
                        AAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGGAGTGATTGGTACTAATGCCNNN                                                                               >  SRR4302518.10270721/1‑101 (MQ=60)
                        AAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGAC                                                                               >  SRR4302518.5123238/1‑101 (MQ=60)
                        AAATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGAC                                                                               <  SRR4302518.5016247/101‑1 (MQ=60)
                         NNNGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACG                                                                              <  SRR4302518.13375098/101‑1 (MQ=60)
                         NATGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGGCCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACN                                                                              <  SRR4302518.4393938/101‑1 (MQ=60)
                           TGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGAC                                                                            <  SRR4302518.223823/101‑1 (MQ=60)
                           TGCTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGAC                                                                            <  SRR4302518.2067483/101‑1 (MQ=60)
                           NNNTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGCC                                                                            <  SRR4302518.9664730/101‑1 (MQ=60)
                            GGTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGNNN                                                                           >  SRR4302518.7907557/1‑101 (MQ=60)
                             CTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGC                                                                          >  SRR4302518.7108670/1‑101 (MQ=60)
                             CTGTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGC                                                                          >  SRR4302518.2146809/1‑101 (MQ=60)
                              NNNCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCG                                                                         <  SRR4302518.9310370/101‑1 (MQ=60)
                               NNNGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCCG                                                                        <  SRR4302518.11079003/101‑1 (MQ=60)
                               GTCGATGGG‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGG                                                                        >  SRR4302518.4917718/1‑101 (MQ=60)
                               GTCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGNNN                                                                        >  SRR4302518.14120480/1‑101 (MQ=60)
                                TCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGcg                                                                       >  SRR4302518.4633403/1‑99 (MQ=60)
                                TCGATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGT                                                                       <  SRR4302518.7256431/101‑1 (MQ=60)
                                   ATGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTNNN                                                                    >  SRR4302518.12606193/1‑101 (MQ=60)
                                    TGGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGNNN                                                                   >  SRR4302518.9980757/1‑101 (MQ=60)
                                     nnntggcggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                                                                      <  SRR4302518.7629040/86‑1 (MQ=60)
                                     nnntggcggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGCG                                                                      <  SRR4302518.11584778/86‑1 (MQ=60)
                                     GGC‑GG‑GGAAAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCNNN                                                                  >  SRR4302518.10445951/1‑101 (MQ=60)
                                         nnnggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGC                                                                  <  SRR4302518.14892535/90‑1 (MQ=60)
                                         GG‑G‑‑AAAG‑G‑CTGCATTATGGGCCGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑Ggaagagataggtaatattgacgacgacgcactgcatcannn                                                             >  SRR4302518.13459906/1‑60 (MQ=45)
                                         GG‑G‑‑AAA‑‑GACTGCATTATGGAGAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGATC‑TGGAAGTGATTGGTACTAATGCCGACGACTCGGTGCCGCATTT                                                              >  SRR4302518.6838426/1‑101 (MQ=60)
                                          gcggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA                                                                 >  SRR4302518.5657693/11‑101 (MQ=60)
                                          gcggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA                                                                 >  SRR4302518.6628485/11‑101 (MQ=60)
                                          gcggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA                                                                 >  SRR4302518.5829268/11‑101 (MQ=60)
                                          G‑G‑‑AAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATNNN                                                             >  SRR4302518.11614517/1‑101 (MQ=60)
                                           nnnggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                                                                <  SRR4302518.8410940/92‑1 (MQ=60)
                                           cggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                                                                <  SRR4302518.4134895/92‑1 (MQ=60)
                                           cggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                                                                <  SRR4302518.582887/92‑1 (MQ=60)
                                           cggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                                                                >  SRR4302518.494987/10‑101 (MQ=60)
                                            nnngaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                                                               <  SRR4302518.12321775/93‑1 (MQ=60)
                                            nnngaaagG‑CTGCATTATGGGCAGTGCGAACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                                                               <  SRR4302518.10713311/93‑1 (MQ=60)
                                            ggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCNNN                                                               >  SRR4302518.8626770/9‑101 (MQ=60)
                                            ggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                                                               <  SRR4302518.730304/93‑1 (MQ=60)
                                            ggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                                                               >  SRR4302518.3374272/9‑101 (MQ=60)
                                            G‑‑AAAG‑G‑CTGCATTATGGGCAGTGCGCCCCAGCGTCTGACAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC                                                            <  SRR4302518.6762132/101‑1 (MQ=60)
                                            G‑‑AAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTGCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTNNN                                                            >  SRR4302518.10594526/1‑101 (MQ=60)
                                             nnnaaagG‑CTGCATTGTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA                                                              <  SRR4302518.10784925/94‑1 (MQ=60)
                                             nnnaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA                                                              <  SRR4302518.12609820/94‑1 (MQ=60)
                                             nnnaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA                                                              <  SRR4302518.12859628/94‑1 (MQ=60)
                                               nnnagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC                                                            <  SRR4302518.10450598/96‑1 (MQ=60)
                                               nnnagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC                                                            <  SRR4302518.9615685/96‑1 (MQ=60)
                                               NNN‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG                                                          <  SRR4302518.8121700/101‑1 (MQ=60)
                                               AAG‑‑G‑CTGCATTATGGGCAGTGCGCCCCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG                                                          <  SRR4302518.5219618/101‑1 (MQ=60)
                                               AAG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAATATCTG                                                          >  SRR4302518.436870/1‑101 (MQ=60)
                                               AAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT                                                           <  SRR4302518.5541774/101‑1 (MQ=60)
                                               AAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT                                                           >  SRR4302518.4034811/1‑101 (MQ=60)
                                               AAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTANNN                                                           >  SRR4302518.11362940/1‑101 (MQ=60)
                                                nnnagtCTGCATTATGGGCAGTGCGCACCAGCGACTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC                                                            <  SRR4302518.12827884/95‑1 (MQ=60)
                                                AG‑‑G‑CTGCATTATGGGCAGTGCGCCCCAGCGTCTGAAAGACCTTCATCCCGTGCGG‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC                                                         <  SRR4302518.4454368/101‑1 (MQ=60)
                                                AG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC                                                         >  SRR4302518.5309199/1‑101 (MQ=60)
                                                AG‑‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC                                                         <  SRR4302518.474628/101‑1 (MQ=60)
                                                AG‑‑G‑CTGCATTATGGGCAGTGCGAACCATCGTCTGAAAGACCTTCATCCCGAGC‑TGGTAGTGATTGGTACTAATGCCTACGACGCGGTGCCGCTTTATCTGC                                                         >  SRR4302518.1692633/1‑101 (MQ=60)
                                                 N‑‑N‑NTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG                                                        <  SRR4302518.8089005/101‑1 (MQ=60)
                                                    G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGGCGACGCGGTGCCGCATTATCTGCGA                                                       >  SRR4302518.949206/1‑101 (MQ=60)
                                                    G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACT‑ATGCCGACGACGCGGTGCCGCCTTATCTGCNNN                                                      >  SRR4302518.13113857/1‑101 (MQ=60)
                                                      CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCNNN                                                      >  SRR4302518.7607875/1‑101 (MQ=60)
                                                      CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCNNN                                                      >  SRR4302518.14896932/1‑101 (MQ=60)
                                                      CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTA                                                      >  SRR4302518.4562770/1‑101 (MQ=60)
                                                      CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTA                                                      >  SRR4302518.5541426/1‑101 (MQ=60)
                                                      CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTA                                                      <  SRR4302518.6534119/101‑1 (MQ=60)
                                                       TGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA                                                     >  SRR4302518.2898941/1‑101 (MQ=60)
                                                        NNNTTATGGGCAGTGCGCACCAGCATCTGAAAGACCTTCATCCCGAGC‑TGGTAGTGATTCGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA                                                    <  SRR4302518.7838421/101‑1 (MQ=60)
                                                        GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAGGGGATTGTTACTAATGCCGACGACGCGGGGCCGCATTATCTGAGTAAC                                                    >  SRR4302518.5513178/1‑101 (MQ=60)
                                                        GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA                                                    <  SRR4302518.6769606/101‑1 (MQ=60)
                                                         NNNTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC                                                   <  SRR4302518.7541530/101‑1 (MQ=60)
                                                         CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGATGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC                                                   >  SRR4302518.7294439/1‑101 (MQ=60)
                                                         CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC                                                   >  SRR4302518.3144972/1‑101 (MQ=60)
                                                         CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCAGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAAT                                                   >  SRR4302518.3930700/1‑101 (MQ=60)
                                                          ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT                                                  <  SRR4302518.4277104/101‑1 (MQ=60)
                                                           nnntggacagtgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTTCCGCATTATCTGCGTAAACTC                                                 <  SRR4302518.10878277/87‑1 (MQ=60)
                                                           TTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTC                                                 >  SRR4302518.102064/1‑101 (MQ=60)
                                                             ATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTA                                               <  SRR4302518.460700/101‑1 (MQ=60)
                                                              TGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAT                                              >  SRR4302518.5235902/1‑101 (MQ=60)
                                                              TGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAT                                              <  SRR4302518.5500087/101‑1 (MQ=60)
                                                              TGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCNNN                                              >  SRR4302518.8628127/1‑101 (MQ=60)
                                                               GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATT                                             >  SRR4302518.2163897/1‑101 (MQ=60)
                                                                 ggagtgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGGGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTTTATNNN                                           >  SRR4302518.13457471/9‑101 (MQ=60)
                                                                 NNNGTGGGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTA                                           <  SRR4302518.12511577/101‑1 (MQ=60)
                                                                  CAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTTCCGCATTATCTGCGTAAACTCTATTNNN                                          >  SRR4302518.10558866/1‑101 (MQ=60)
                                                                   attgcgCACCAGCGTCTCAAAGACCTTCATCCCGCGC‑TCGAAGTGATTGGTGCTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTACC                                         <  SRR4302518.3627475/95‑1 (MQ=60)
                                                                   AGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATC                                         >  SRR4302518.6060164/1‑101 (MQ=60)
                                                                   AGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATCCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATC                                         <  SRR4302518.6905186/101‑1 (MQ=60)
                                                                    gtgcgccCCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCG                                        <  SRR4302518.6490449/94‑1 (MQ=60)
                                                                    gtgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGGGGTTGGTACTAATGCCGACGACGCGGGGCCGGATTTTCGTCGTAAACTCTATTTttct                                        >  SRR4302518.4693674/6‑97 (MQ=54)
                                                                    gcgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTANNN                                        >  SRR4302518.11145059/6‑101 (MQ=60)
                                                                        NNNCCAGCGTCTGAAAGCCCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTACT                                    <  SRR4302518.11615622/101‑1 (MQ=60)
                                                                        GCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAT                                    >  SRR4302518.3529420/1‑101 (MQ=60)
                                                                         CACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATC                                   <  SRR4302518.2391724/101‑1 (MQ=60)
                                                                         CACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAACC                                   >  SRR4302518.5933895/1‑101 (MQ=60)
                                                                          ACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCG                                  >  SRR4302518.1212388/1‑101 (MQ=60)
                                                                          ACCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCG                                  >  SRR4302518.2412868/1‑101 (MQ=60)
                                                                           CCAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGT                                 <  SRR4302518.3604399/101‑1 (MQ=60)
                                                                            NNNCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTT                                <  SRR4302518.12108338/101‑1 (MQ=60)
                                                                            CAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTT                                <  SRR4302518.6399278/101‑1 (MQ=60)
                                                                            CAGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTT                                <  SRR4302518.1939908/101‑1 (MQ=60)
                                                                             AGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTC                               >  SRR4302518.160803/1‑101 (MQ=60)
                                                                             AGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTC                               >  SRR4302518.6946148/1‑101 (MQ=60)
                                                                             AGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGNNN                               >  SRR4302518.13913285/1‑101 (MQ=60)
                                                                             AGCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGNNN                               >  SRR4302518.13084822/1‑101 (MQ=60)
                                                                             AGCGTCTGAAAGAACTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGNNN                               >  SRR4302518.11952700/1‑101 (MQ=60)
                                                                              GCGTCTGAAAGCCCTTCACCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCT                              <  SRR4302518.4694070/101‑1 (MQ=60)
                                                                              GCGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCT                              <  SRR4302518.4659819/101‑1 (MQ=60)
                                                                               NNNCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCCT                             <  SRR4302518.10068621/101‑1 (MQ=60)
                                                                               CGTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                             >  SRR4302518.3735516/1‑101 (MQ=60)
                                                                               CGTCGGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                             <  SRR4302518.4298558/101‑1 (MQ=60)
                                                                                GTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCTTAAACTCTATTTATCGTAATCGTTCNNN                            >  SRR4302518.10563236/1‑101 (MQ=60)
                                                                                GTCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTT                            >  SRR4302518.6499891/1‑101 (MQ=60)
                                                                                 TCTGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTNNN                           >  SRR4302518.7547557/1‑101 (MQ=60)
                                                                                 NNNGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTA                           <  SRR4302518.11570452/101‑1 (MQ=60)
                                                                                   TGAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATT                         <  SRR4302518.127694/101‑1 (MQ=60)
                                                                                    nnnagcCCTTCATCCCGCGC‑TGGAAGTGATTGGTACTAATACCGACGACGCGGTACCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                        <  SRR4302518.10787788/95‑1 (MQ=60)
                                                                                    GAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                        <  SRR4302518.1947615/101‑1 (MQ=60)
                                                                                    GAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                        <  SRR4302518.3157858/101‑1 (MQ=60)
                                                                                    GAAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAAGGCCGCCGAAGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCTTAATCGATCTTTANNN                        >  SRR4302518.13208046/1‑101 (MQ=60)
                                                                                     NNNGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTG                       <  SRR4302518.11999137/101‑1 (MQ=60)
                                                                                     AAAGACCTTCATCCCGAGC‑TGGAAGTGATTGGGACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATGTATCGTAATCGTTCTTTATTTG                       >  SRR4302518.111737/1‑101 (MQ=60)
                                                                                      NNNACCTTCATCCCTAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGG                      <  SRR4302518.13776513/101‑1 (MQ=60)
                                                                                      AAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGGCGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTNNN                      >  SRR4302518.8035355/1‑101 (MQ=60)
                                                                                      AAGACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGATCTTTATTNNN                      >  SRR4302518.8817204/1‑101 (MQ=60)
                                                                                        GACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTC                    <  SRR4302518.2937340/101‑1 (MQ=60)
                                                                                        GACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTC                    <  SRR4302518.2221168/101‑1 (MQ=60)
                                                                                        GACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTC                    <  SRR4302518.663233/101‑1 (MQ=60)
                                                                                         ACCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCA                   <  SRR4302518.6468212/101‑1 (MQ=60)
                                                                                          CCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTNNN                  >  SRR4302518.8017776/1‑101 (MQ=60)
                                                                                          CCTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTNNN                  >  SRR4302518.14543632/1‑101 (MQ=60)
                                                                                           GTTCATCCCGAGC‑TGGAAGTGATTGGGACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGCAATCGTTCTTTATTTGGTCNNN                 >  SRR4302518.9220081/1‑101 (MQ=60)
                                                                                           CTTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCNNN                 >  SRR4302518.14481893/1‑101 (MQ=60)
                                                                                            nnnacCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT                <  SRR4302518.14735657/96‑1 (MQ=60)
                                                                                            TTCATCCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCANNN                >  SRR4302518.7359617/1‑101 (MQ=60)
                                                                                            ATCATCCCGAGC‑TGGAAGGGATTGGGACTAATGCCGACGACGCGGTCCCGCATTATCTGCGTAAACTCTATTCATCGTAATAGTTCTTTATTTGGTCANNN                >  SRR4302518.14414522/1‑101 (MQ=60)
                                                                                             TCATCCCGAGC‑TGGATGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTG               <  SRR4302518.5097268/101‑1 (MQ=60)
                                                                                                 CCCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAA           >  SRR4302518.1981109/1‑101 (MQ=60)
                                                                                                  CCGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGcacagtagtcnnn          >  SRR4302518.10930366/1‑88 (MQ=60)
                                                                                                   nnnagctgGAAGTGATTGGTTCTAATGCCGACGACGCGGTGCCGCATTATCTGAGTAAACTCTATTTTTCGTAATTGTACTTTATTTGGTCAGTTGTAAAC          <  SRR4302518.7557870/93‑1 (MQ=60)
                                                                                                   CGAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACC         <  SRR4302518.281317/101‑1 (MQ=60)
                                                                                                    GAGC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCT        <  SRR4302518.5280340/101‑1 (MQ=60)
                                                                                                      nnntgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTAAGTTGTCAACCTG       <  SRR4302518.14039778/96‑1 (MQ=60)
                                                                                                      NN‑N‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGAT     <  SRR4302518.8940653/101‑1 (MQ=60)
                                                                                                      GC‑TGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGA      <  SRR4302518.4078922/101‑1 (MQ=60)
                                                                                                       ggtgGAAGTGATTGGTACTAATGCCGACGACCCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGAAATCGATCGTTATTTGGTCAGTTGTCAACCNNN      >  SRR4302518.13649259/5‑101 (MQ=60)
                                                                                                        ctgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTNNN     >  SRR4302518.9536673/4‑101 (MQ=60)
                                                                                                           GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATNNN  >  SRR4302518.13973227/1‑101 (MQ=60)
                                                                                                           |                                                                                                    
GTTGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GG‑G‑‑AAA‑‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGC‑T‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACCTGATACT  >  NC_000913/4008427‑4008625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑