Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	653,656	G→A	R191H (CGC→CAC)	citB →	CitB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	653,656	0	G	A	99.6%	799.2 / ‑5.8	220	R191H (CGC→CAC)	citB	CitB
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (108/111); total (108/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 231 total aligned reads displayed.

CCTTGACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAAC  >  NC_000913/653566‑653745

 cTTGACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCacac                                                                                        >  2:4288125/1‑93 (MQ=255)

 cTTGACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCacac                                                                                        <  1:2637146/93‑1 (MQ=255)

   tGACGCTAAACGCGGTGCGAAAACTGTTTAAGGAGCCTGGTGTGCAAAATACGGCAGAAACGGTGGCGCAGGCACTTACCATCAGCCAcacca                                                                                      <  1:3403707/93‑1 (MQ=255)

   tGACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCAcacca                                                                                      >  2:2582322/1‑93 (MQ=255)

    gACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCAcaccac                                                                                     >  1:2402405/1‑93 (MQ=255)

    gACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCAcaccac                                                                                     <  1:5225010/93‑1 (MQ=255)

     aCGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACt                                                                                    <  1:3166816/93‑1 (MQ=255)

      cGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTg                                                                                   >  1:5803495/1‑93 (MQ=255)

       gCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGc                                                                                  >  1:145124/1‑93 (MQ=255)

         tAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCa                                                                                <  2:5441010/93‑1 (MQ=255)

            aCGGGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGCCACTGACCATCAGCCACACCACTGCCAGGc                                                                             <  1:6163655/93‑1 (MQ=255)

            aCGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGc                                                                             <  2:2171435/93‑1 (MQ=255)

              gcgGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGt                                                                           >  2:5288673/1‑93 (MQ=255)

              gcgGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGt                                                                           <  1:3039028/93‑1 (MQ=255)

              gcgGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGt                                                                           <  1:2933134/93‑1 (MQ=255)

              gcgGTGCGAAAACTGTTTAAAGAGCCTGGAGTGCCACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACAACACTGCCAGGCGt                                                                           >  2:3180906/1‑93 (MQ=255)

                ggTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTa                                                                         >  1:4940847/1‑93 (MQ=255)

                ggTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTa                                                                         <  2:1255269/93‑1 (MQ=255)

                  tGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATc                                                                       >  2:442717/1‑93 (MQ=255)

                    cGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCtt                                                                     <  1:2727765/93‑1 (MQ=255)

                     gAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTg                                                                    >  2:1579948/1‑93 (MQ=255)

                     gAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATATTg                                                                    >  1:4455045/1‑93 (MQ=255)

                      aaaaCTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGa                                                                   >  2:6068628/1‑93 (MQ=255)

                       aaaCTGTTTAAAGAGCCTGGTGTGCAACATACGGCGGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGaa                                                                  <  1:1614833/93‑1 (MQ=255)

                       aaaCTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGaa                                                                  <  2:3968305/93‑1 (MQ=255)

                       aaaCTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGaa                                                                  <  2:4082729/93‑1 (MQ=255)

                        aaCTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAat                                                                 >  1:2849318/1‑93 (MQ=255)

                        aaCTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAat                                                                 <  1:1745629/93‑1 (MQ=255)

                         aCTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAata                                                                <  1:5777881/93‑1 (MQ=255)

                          cTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAatat                                                               <  1:3007654/93‑1 (MQ=255)

                           tGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATAtt                                                              <  2:3306730/93‑1 (MQ=255)

                            gTTTAAAGAGCCTGGTGTGCAGCATACGGCAGAAACGGTGGCGCAGGCACTGCCCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTg                                                             <  1:3105530/93‑1 (MQ=255)

                             tttAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTgc                                                            <  1:1010814/93‑1 (MQ=255)

                             gttAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTgc                                                            <  1:674319/92‑1 (MQ=255)

                               tAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTgcgc                                                          >  2:1918861/1‑93 (MQ=255)

                                     gCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAgccg                                                    >  1:1342640/1‑93 (MQ=255)

                                      ccTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAgccgc                                                   >  2:5182583/1‑93 (MQ=255)

                                       cTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAgccgcc                                                  >  2:5682918/1‑93 (MQ=255)

                                        tGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCa                                                 >  2:3230450/1‑93 (MQ=255)

                                        tGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCa                                                 <  1:6301831/93‑1 (MQ=255)

                                          gtgtGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATc                                               >  2:2344465/1‑93 (MQ=255)

                                          gtgtGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATc                                               >  1:5409794/1‑93 (MQ=255)

                                           tgtgCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCt                                              <  1:510070/93‑1 (MQ=255)

                                             tgCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGa                                            >  1:5042301/1‑93 (MQ=255)

                                               cAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGatt                                          >  2:2629647/1‑93 (MQ=255)

                                                aaCATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGatta                                         >  1:4014568/1‑93 (MQ=255)

                                                aaCATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGatta                                         >  1:6006665/1‑93 (MQ=255)

                                                aaCATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGatta                                         >  2:1821250/1‑93 (MQ=255)

                                                aaCATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGatta                                         <  1:2239295/93‑1 (MQ=255)

                                                aaCATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGatta                                         <  1:3652760/93‑1 (MQ=255)

                                                  cATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGattatt                                       <  1:4266674/93‑1 (MQ=255)

                                                  cATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGattatt                                       >  2:287121/1‑93 (MQ=255)

                                                  cATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGattatt                                       <  1:1812288/93‑1 (MQ=255)

                                                  cATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGattatt                                       <  2:1077742/93‑1 (MQ=255)

                                                   aTACGGCAGAAACGGTGGCGCAGGCGCTGACCATCAGCCACACCACTGCAAGGCCTTATCTAGAATATTGCGCCAGCCGCCATCTGATTATTg                                      <  2:5777986/93‑1 (MQ=255)

                                                    tACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGc                                     >  1:5689059/1‑93 (MQ=255)

                                                    tACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGc                                     >  2:2909379/1‑93 (MQ=255)

                                                     aCGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCt                                    <  1:3841071/93‑1 (MQ=255)

                                                      cGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGCATATTGCGCCAGCCGCCATCTGATTATTGCTg                                   >  2:1757226/1‑93 (MQ=255)

                                                      cGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTg                                   >  1:3919276/1‑93 (MQ=255)

                                                       ggCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGa                                  <  2:2830123/93‑1 (MQ=255)

                                                       ggCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGa                                  <  2:112899/93‑1 (MQ=255)

                                                       ggCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGa                                  >  1:451546/1‑93 (MQ=255)

                                                         cAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGaaa                                <  2:10109/93‑1 (MQ=255)

                                                         cAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGaaa                                <  1:2522272/93‑1 (MQ=255)

                                                            aaaCGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTg                             >  1:4131117/1‑93 (MQ=255)

                                                              aCGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGtt                           >  2:4722161/1‑93 (MQ=255)

                                                                ggTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCa                         <  1:6183107/93‑1 (MQ=255)

                                                                ggTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCa                         <  2:5565292/93‑1 (MQ=255)

                                                                 gTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCAc                        <  1:5436202/93‑1 (MQ=255)

                                                                 gTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCAc                        <  1:4232802/93‑1 (MQ=255)

                                                                 gTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCAc                        >  1:399089/1‑93 (MQ=255)

                                                                 gTGGCGCAGGCACTGACCATCAGCCACACCACGGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCAc                        <  1:1578205/93‑1 (MQ=255)

                                                                  tGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACg                       <  2:3575461/93‑1 (MQ=255)

                                                                  tGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACg                       <  2:5832323/93‑1 (MQ=255)

                                                                  tGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACg                       <  1:1131428/93‑1 (MQ=255)

                                                                   ggCGCAGGCACTTACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACgg                      <  1:4780226/93‑1 (MQ=255)

                                                                   ggCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACgg                      <  1:6277243/93‑1 (MQ=255)

                                                                       cAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGCTCACGGCaaa                  >  1:2109555/1‑93 (MQ=255)

                                                                        aGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAg                 >  1:2738156/1‑93 (MQ=255)

                                                                         ggCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGt                <  1:1992961/93‑1 (MQ=255)

                                                                         ggCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGt                <  2:2599175/93‑1 (MQ=255)

                                                                          gCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGtt               <  1:5249890/93‑1 (MQ=255)

                                                                          gCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGtt               <  1:3755360/93‑1 (MQ=255)

                                                                           cACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTg              >  1:1921357/1‑93 (MQ=255)

                                                                              tGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCa           >  1:4650067/1‑93 (MQ=255)

                                                                              tGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCa           <  1:3720735/93‑1 (MQ=255)

                                                                              tGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCa           <  1:3368915/93‑1 (MQ=255)

                                                                               gACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAg          >  2:662711/1‑93 (MQ=255)

                                                                               gACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAg          >  1:1295188/1‑93 (MQ=255)

                                                                               gACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAg          <  2:4599974/93‑1 (MQ=255)

                                                                                aCCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAGGTTGGCAGa         <  2:5327312/93‑1 (MQ=255)

                                                                                 ccATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGAc        >  1:1198328/1‑93 (MQ=255)

                                                                                 ccATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGAc        <  2:1375064/93‑1 (MQ=255)

                                                                                 ccATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGAc        <  2:2131015/93‑1 (MQ=255)

                                                                                 ccATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGAc        >  2:5786248/1‑93 (MQ=255)

                                                                                    tCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACcac     >  2:4970758/1‑93 (MQ=255)

                                                                                      aGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACaa   >  1:1208791/1‑93 (MQ=255)

                                                                                      aGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACaa   <  2:250529/93‑1 (MQ=255)

                                                                                       gCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAAc  >  1:777850/1‑93 (MQ=255)

CCTTGACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAAC  >  NC_000913/653566‑653745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACCCCTTGACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCAT  >  NC_000913/653563‑653749

ACCCCTTGACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCA                                                                                               >  SRR4302533.3739840/1‑94 (MQ=60)

ACCCCTTGACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCA                                                                                               <  SRR4302533.412599/94‑1 (MQ=60)

 CCCCTTGACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCAC                                                                                              >  SRR4302533.2804253/1‑94 (MQ=60)

    CTTGACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACC                                                                                           <  SRR4302533.2653355/94‑1 (MQ=60)

    CTTGACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACC                                                                                           <  SRR4302533.453973/94‑1 (MQ=60)

     TTGACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCA                                                                                          >  SRR4302533.3113942/1‑94 (MQ=60)

      TGACGCTAAACGCGGTGCGAAAACTGTTTAAGGAGCCTGGTGTGCAAAATACGGCAGAAACGGTGGCGCAGGCACTTACCATCAGCCACACCAC                                                                                         <  SRR4302533.3424724/94‑1 (MQ=60)

      TGACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCAC                                                                                         >  SRR4302533.2417243/1‑94 (MQ=60)

       GACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACT                                                                                        <  SRR4302533.728778/94‑1 (MQ=60)

       GACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACT                                                                                        <  SRR4302533.5256454/94‑1 (MQ=60)

       GACGCTAAACGCGGTGCGAAAACTGTTGAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACT                                                                                        <  SRR4302533.5869574/94‑1 (MQ=60)

        ACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTG                                                                                       <  SRR4302533.3186301/94‑1 (MQ=60)

        ACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTG                                                                                       >  SRR4302533.5838548/1‑94 (MQ=60)

         CGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGC                                                                                      >  SRR4302533.146054/1‑94 (MQ=60)

            TAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAG                                                                                   >  SRR4302533.3749228/1‑94 (MQ=60)

            TAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAG                                                                                   >  SRR4302533.5262178/1‑94 (MQ=60)

             AAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGG                                                                                  >  SRR4302533.2887023/1‑94 (MQ=60)

             AAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGG                                                                                  <  SRR4302533.1655762/94‑1 (MQ=60)

              AACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGC                                                                                 >  SRR4302533.384065/1‑94 (MQ=60)

              AACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGC                                                                                 >  SRR4302533.1597072/1‑94 (MQ=60)

              AACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGC                                                                                 <  SRR4302533.620809/94‑1 (MQ=60)

               ACGGGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGCCACTGACCATCAGCCACACCACTGCCAGGCG                                                                                <  SRR4302533.6200711/94‑1 (MQ=60)

               ACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCG                                                                                <  SRR4302533.1512594/94‑1 (MQ=60)

                CGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGT                                                                               <  SRR4302533.1366304/94‑1 (MQ=60)

                 GCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTT                                                                              <  SRR4302533.3057625/94‑1 (MQ=60)

                 GCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTT                                                                              <  SRR4302533.2951071/94‑1 (MQ=60)

                 GCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTT                                                                              <  SRR4302533.3963845/94‑1 (MQ=60)

                  CGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTA                                                                             >  SRR4302533.4970758/1‑94 (MQ=60)

                    GTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATT                                                                           >  SRR4302533.1334047/1‑94 (MQ=60)

                       CGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTG                                                                        <  SRR4302533.2744499/94‑1 (MQ=60)

                       CGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATATTG                                                                        >  SRR4302533.4481982/1‑94 (MQ=60)

                       CGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGACAGGCGTTATCTTG                                                                        >  SRR4302533.6223869/1‑94 (MQ=60)

                          AAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCGGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAAT                                                                     <  SRR4302533.1624623/94‑1 (MQ=60)

                          AAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAAT                                                                     >  SRR4302533.2866849/1‑94 (MQ=60)

                          AAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAAT                                                                     >  SRR4302533.2866850/1‑94 (MQ=60)

                           AACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATA                                                                    <  SRR4302533.6227959/94‑1 (MQ=60)

                           AACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATA                                                                    <  SRR4302533.1756248/94‑1 (MQ=60)

                           AACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATA                                                                    >  SRR4302533.5728011/1‑94 (MQ=60)

                            ACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATAT                                                                   <  SRR4302533.4003845/94‑1 (MQ=60)

                            ACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATAT                                                                   <  SRR4302533.5812781/94‑1 (MQ=60)

                            ACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATAT                                                                   <  SRR4302533.5833040/94‑1 (MQ=60)

                             CTGTTTAACGAGCCTGGTGTGNCACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATT                                                                  <  SRR4302533.2642970/94‑1 (MQ=60)

                             CTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATT                                                                  <  SRR4302533.3026052/94‑1 (MQ=60)

                             CTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATT                                                                  >  SRR4302533.5280469/1‑94 (MQ=60)

                             CTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATT                                                                  >  SRR4302533.5462740/1‑94 (MQ=60)

                               GTTTAAAGAGCCTGGTGTGCAGCATACGGCAGAAACGGTGGCGCAGGCACTGCCCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGC                                                                <  SRR4302533.3124619/94‑1 (MQ=60)

                               GTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGC                                                                >  SRR4302533.3677578/1‑94 (MQ=60)

                                TTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCG                                                               <  SRR4302533.3391861/94‑1 (MQ=60)

                                TTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCG                                                               <  SRR4302533.1016728/94‑1 (MQ=60)

                                GTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCG                                                               <  SRR4302533.678300/94‑1 (MQ=60)

                                   AAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCA                                                            <  SRR4302533.4407016/94‑1 (MQ=60)

                                   AAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCA                                                            >  SRR4302533.1228016/1‑94 (MQ=60)

                                    AAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAG                                                           <  SRR4302533.1129007/94‑1 (MQ=60)

                                     AGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGC                                                          <  SRR4302533.4754867/94‑1 (MQ=60)

                                     AGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGC                                                          >  SRR4302533.4176258/1‑94 (MQ=60)

                                       AGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCG                                                        >  SRR4302533.1350724/1‑94 (MQ=60)

                                        GCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGC                                                       >  SRR4302533.4872990/1‑94 (MQ=60)

                                         CCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCC                                                      >  SRR4302533.2143870/1‑94 (MQ=60)

                                          CTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCA                                                     >  SRR4302533.3324900/1‑94 (MQ=60)

                                           TGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCAT                                                    >  SRR4302533.2609547/1‑94 (MQ=60)

                                           TGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCAT                                                    <  SRR4302533.6339475/94‑1 (MQ=60)

                                            GGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATC                                                   >  SRR4302533.5442315/1‑94 (MQ=60)

                                            GGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATC                                                   >  SRR4302533.3046625/1‑94 (MQ=60)

                                             GTGTGCAACATACGGCAGAAACGGTGGGGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCT                                                  >  SRR4302533.393835/1‑94 (MQ=60)

                                             GTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCT                                                  <  SRR4302533.4588409/94‑1 (MQ=60)

                                             GTGTGCAACAAACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCT                                                  <  SRR4302533.654049/94‑1 (MQ=60)

                                              TGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTG                                                 <  SRR4302533.513101/94‑1 (MQ=60)

                                              TGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTG                                                 >  SRR4302533.4282007/1‑94 (MQ=60)

                                               GTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGA                                                >  SRR4302533.5072816/1‑94 (MQ=60)

                                                  CAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTA                                             >  SRR4302533.4039017/1‑94 (MQ=60)

                                                  CAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTA                                             >  SRR4302533.6043057/1‑94 (MQ=60)

                                                  CAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTA                                             <  SRR4302533.2966665/94‑1 (MQ=60)

                                                  CAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTA                                             <  SRR4302533.2727842/94‑1 (MQ=60)

                                                   AACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTAT                                            >  SRR4302533.3759950/1‑94 (MQ=60)

                                                   AACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTAT                                            <  SRR4302533.3675220/94‑1 (MQ=60)

                                                   AACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTAT                                            <  SRR4302533.2253134/94‑1 (MQ=60)

                                                     CATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTG                                          <  SRR4302533.1823358/94‑1 (MQ=60)

                                                     CATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTG                                          <  SRR4302533.4292367/94‑1 (MQ=60)

                                                      ATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGC                                         >  SRR4302533.5723421/1‑94 (MQ=60)

                                                      ATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGC                                         >  SRR4302533.5042385/1‑94 (MQ=60)

                                                       TACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCT                                        >  SRR4302533.1994937/1‑94 (MQ=60)

                                                        ACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTG                                       <  SRR4302533.3864535/94‑1 (MQ=60)

                                                        ACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTG                                       >  SRR4302533.3943212/1‑94 (MQ=60)

                                                         CGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGA                                      <  SRR4302533.4836783/94‑1 (MQ=60)

                                                         CGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGA                                      >  SRR4302533.454247/1‑94 (MQ=60)

                                                         CGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGA                                      <  SRR4302533.4138088/94‑1 (MQ=60)

                                                          GGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAA                                     <  SRR4302533.2751937/94‑1 (MQ=60)

                                                            GAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCGAGGCGTTATCTTGAATATTGCGCGAGCCGCCATCTGATTATTGCTGAAAT                                   <  SRR4302533.945270/94‑1 (MQ=60)

                                                            CAGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAAT                                   <  SRR4302533.2537813/94‑1 (MQ=60)

                                                             AGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAACTT                                  >  SRR4302533.2057897/1‑94 (MQ=60)

                                                             AGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATT                                  <  SRR4302533.3048528/94‑1 (MQ=60)

                                                             AGAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATT                                  >  SRR4302533.2780870/1‑94 (MQ=60)

                                                              GAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTG                                 <  SRR4302533.510746/94‑1 (MQ=60)

                                                              GAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTG                                 >  SRR4302533.6326890/1‑94 (MQ=60)

                                                              GAAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTG                                 >  SRR4302533.4156192/1‑94 (MQ=60)

                                                               AAACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGT                                >  SRR4302533.2028752/1‑94 (MQ=60)

                                                                 ACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTC                              <  SRR4302533.351100/94‑1 (MQ=60)

                                                                 ACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTC                              >  SRR4302533.5505601/1‑94 (MQ=60)

                                                                 ACGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTC                              <  SRR4302533.1214753/94‑1 (MQ=60)

                                                                  CGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCGATCTGATTATTGCTGAAATTGTTCA                             <  SRR4302533.4032161/94‑1 (MQ=60)

                                                                  CGGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCA                             >  SRR4302533.4203226/1‑94 (MQ=60)

                                                                   GGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCAC                            >  SRR4302533.401500/1‑94 (MQ=60)

                                                                   GGTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCAC                            <  SRR4302533.6220233/94‑1 (MQ=60)

                                                                    GTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACG                           <  SRR4302533.5468940/94‑1 (MQ=60)

                                                                    GTGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACG                           <  SRR4302533.4258383/94‑1 (MQ=60)

                                                                    GTGGCGCAGGCACTGACCATCAGCCACACCACGGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACG                           <  SRR4302533.1587709/94‑1 (MQ=60)

                                                                     TGGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGG                          <  SRR4302533.1138156/94‑1 (MQ=60)

                                                                      GGCGCAGGCGCTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGC                         <  SRR4302533.1784126/94‑1 (MQ=60)

                                                                      GGCGCAGGCACTTACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGC                         <  SRR4302533.4809184/94‑1 (MQ=60)

                                                                      GGCGCAGGCACTGACCATCAGCCACGCCACTGCCAGGCTTTATCTTCAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGC                         <  SRR4302533.4915866/94‑1 (MQ=60)

                                                                      GGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGC                         <  SRR4302533.6314723/94‑1 (MQ=60)

                                                                      GGCGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGC                         <  SRR4302533.2502039/94‑1 (MQ=60)

                                                                        CGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAA                       >  SRR4302533.1198482/1‑94 (MQ=60)

                                                                        CGCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAA                       >  SRR4302533.5918802/1‑94 (MQ=60)

                                                                         GCAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGCTCACGGCAAA                      >  SRR4302533.2122568/1‑94 (MQ=60)

                                                                          CAGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAG                     >  SRR4302533.2754946/1‑94 (MQ=60)

                                                                           AGGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGT                    <  SRR4302533.896886/94‑1 (MQ=60)

                                                                            GGCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTT                   <  SRR4302533.2005183/94‑1 (MQ=60)

                                                                             GCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTG                  >  SRR4302533.1933086/1‑94 (MQ=60)

                                                                             GCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTG                  <  SRR4302533.3778376/94‑1 (MQ=60)

                                                                             GCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTG                  <  SRR4302533.5281442/94‑1 (MQ=60)

                                                                             GCACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTG                  >  SRR4302533.2322858/1‑94 (MQ=60)

                                                                              CACTGACCATCAGCCCCACCACTGCCAGGCGTTATCTTGAATATTGCCCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGG                 <  SRR4302533.192019/94‑1 (MQ=60)

                                                                              CACTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGG                 >  SRR4302533.6175413/1‑94 (MQ=60)

                                                                                CTGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCA               >  SRR4302533.4678242/1‑94 (MQ=60)

                                                                                 TGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAG              <  SRR4302533.4515354/94‑1 (MQ=60)

                                                                                 TGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAG              <  SRR4302533.3743569/94‑1 (MQ=60)

                                                                                 TGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAG              <  SRR4302533.3389722/94‑1 (MQ=60)

                                                                                 TGACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAG              >  SRR4302533.1302985/1‑94 (MQ=60)

                                                                                   ACCATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGAC            >  SRR4302533.1205528/1‑94 (MQ=60)

                                                                                     CATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCA          <  SRR4302533.5234101/94‑1 (MQ=60)

                                                                                     CATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCA          >  SRR4302533.1484619/1‑94 (MQ=60)

                                                                                      ATCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCAC         >  SRR4302533.5617961/1‑94 (MQ=60)

                                                                                       TCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACA        <  SRR4302533.1293792/94‑1 (MQ=60)

                                                                                       TCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACA        >  SRR4302533.5383079/1‑94 (MQ=60)

                                                                                       TCAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACA        <  SRR4302533.3327610/94‑1 (MQ=60)

                                                                                        CAGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAA       >  SRR4302533.1216051/1‑94 (MQ=60)

                                                                                         AGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAAC      <  SRR4302533.176119/94‑1 (MQ=60)

                                                                                         AGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAAC      >  SRR4302533.782427/1‑94 (MQ=60)

                                                                                         AGCCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAAC      >  SRR4302533.317760/1‑94 (MQ=60)

                                                                                           CCACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGC    >  SRR4302533.2741805/1‑94 (MQ=60)

                                                                                            CACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCA   >  SRR4302533.5407056/1‑94 (MQ=60)

                                                                                             ACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCAT  >  SRR4302533.5265322/1‑94 (MQ=60)

                                                                                             ACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCAT  <  SRR4302533.2473205/94‑1 (MQ=60)

                                                                                             ACACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCAT  <  SRR4302533.3979058/94‑1 (MQ=60)

ACCCCTTGACGCTAAACGCGGTGCGAAAACTGTTTAAAGAGCCTGGTGTGCAACATACGGCAGAAACGGTGGCGCAGGCACTGACCATCAGCCGCACCACTGCCAGGCGTTATCTTGAATATTGCGCCAGCCGCCATCTGATTATTGCTGAAATTGTTCACGGCAAAGTTGGCAGACCACAACGCAT  >  NC_000913/653563‑653749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`