Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	657,814	G→A	A113T (GCC→ACC)	ybeM →	putative amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	657,814	0	G	A	99.5%	758.7 / NA	216	A113T (GCC→ACC)	ybeM	putative amidase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (88/127); minor base C (0/1); total (88/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 231 total aligned reads displayed.

GCACAGGCATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTGCCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAGATCCC  >  NC_000913/657724‑657903

gcACAGGCATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTAcc                                                                                         >  1:3114814/1‑93 (MQ=255)

  acaGGCATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGc                                                                                       >  1:3506443/1‑93 (MQ=255)

  acaGGCATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGc                                                                                       <  2:2815220/93‑1 (MQ=255)

    aGGCATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTc                                                                                     >  1:25933/1‑93 (MQ=255)

     ggCATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCg                                                                                    >  2:4403872/1‑93 (MQ=255)

       cATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTg                                                                                  <  2:3669373/93‑1 (MQ=255)

        aTTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTgc                                                                                 >  1:2309333/1‑93 (MQ=255)

         ttACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTgcg                                                                                >  2:5250172/1‑93 (MQ=255)

         ttACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTgcg                                                                                >  1:807047/1‑93 (MQ=255)

           aCAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCt                                                                              <  2:5437632/93‑1 (MQ=255)

           aCAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCt                                                                              <  2:5683195/93‑1 (MQ=255)

           aCAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCt                                                                              <  2:5870585/93‑1 (MQ=255)

            cAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTg                                                                             <  1:6078880/93‑1 (MQ=255)

             aGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTgg                                                                            >  2:4529066/1‑93 (MQ=255)

              ttgAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGa                                                                           <  1:1723315/91‑1 (MQ=255)

              gggAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGa                                                                           <  1:4648159/93‑1 (MQ=255)

              gggAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGa                                                                           <  1:2800538/93‑1 (MQ=255)

               ggAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGAt                                                                          >  1:1072277/1‑93 (MQ=255)

                   cTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATacca                                                                      <  2:4281226/93‑1 (MQ=255)

                     gAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATaccacc                                                                    <  2:390973/93‑1 (MQ=255)

                       aaTCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTg                                                                  >  1:4516566/1‑93 (MQ=255)

                           cTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTtat                                                              <  1:6140149/93‑1 (MQ=255)

                           cTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTtat                                                              >  1:5286477/1‑93 (MQ=255)

                             ggTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTatat                                                            <  2:4130616/93‑1 (MQ=255)

                                tcTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATAtggt                                                         <  1:2305293/92‑1 (MQ=255)

                                aCTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATAtggt                                                         <  1:5885810/93‑1 (MQ=255)

                                aCTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATAtggt                                                         >  2:866505/1‑93 (MQ=255)

                                aCTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATAtggt                                                         <  2:5199646/93‑1 (MQ=255)

                                aCTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATAtggt                                                         <  1:3980156/93‑1 (MQ=255)

                                 cTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTCCCGCTCGTGCGCTGGATACCACCTGTTATAtggtg                                                        <  1:278649/93‑1 (MQ=255)

                                 cTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATAtggtg                                                        <  2:561858/93‑1 (MQ=255)

                                 cTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATAtggtg                                                        <  2:500704/93‑1 (MQ=255)

                                   tCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTggc                                                      <  1:5576993/93‑1 (MQ=255)

                                     cTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTggcgg                                                    <  1:2121756/93‑1 (MQ=255)

                                      tGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTggcggc                                                   <  1:4752440/93‑1 (MQ=255)

                                        ccgccTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGcggcgg                                                 <  2:1376196/93‑1 (MQ=255)

                                         cgccTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCggg                                                <  2:898330/93‑1 (MQ=255)

                                          gccTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCgggg                                               >  1:2845148/1‑93 (MQ=255)

                                          gccTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCgggg                                               <  1:754354/93‑1 (MQ=255)

                                           ccTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCggggg                                              <  2:3607911/93‑1 (MQ=255)

                                            cTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGa                                             <  2:350472/93‑1 (MQ=255)

                                             tGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGGTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAg                                            >  1:4441004/1‑93 (MQ=255)

                                             tGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAg                                            >  2:3932897/1‑93 (MQ=255)

                                             tGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAg                                            >  1:1051540/1‑93 (MQ=255)

                                             tGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAg                                            <  1:888789/93‑1 (MQ=255)

                                              gggTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATTGTGGCGGCGGGGGAGt                                           >  2:767193/1‑93 (MQ=255)

                                               ggTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTg                                          >  1:2239351/1‑93 (MQ=255)

                                               ggTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTg                                          >  1:255521/1‑93 (MQ=255)

                                                gTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGc                                         <  2:3799858/93‑1 (MQ=255)

                                                 ttCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCg                                        <  2:1359982/93‑1 (MQ=255)

                                                 ttCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCg                                        <  1:1287790/93‑1 (MQ=255)

                                                  tCGCGAGCGGCTCAAAGAGCATCGCTGGTCAACGTTACTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCgg                                       <  2:6305253/93‑1 (MQ=255)

                                                    gcgGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGa                                     <  1:3256225/93‑1 (MQ=255)

                                                    gcgGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGa                                     >  2:376432/1‑93 (MQ=255)

                                                    gcgGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGa                                     <  1:4868915/93‑1 (MQ=255)

                                                     cgGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGaa                                    <  2:477218/93‑1 (MQ=255)

                                                      gggCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGCGCGGGAAc                                   >  2:491979/1‑93 (MQ=255)

                                                       ggCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGGGCGGGAACa                                  >  1:5491094/1‑93 (MQ=255)

                                                         ccGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACaaa                                <  2:4363104/93‑1 (MQ=255)

                                                          cGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGCGGCGGCGGGGGACTGCGGCAACaaaa                               >  2:2154027/1‑93 (MQ=255)

                                                           gCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACaaaaa                              <  1:2371954/93‑1 (MQ=255)

                                                             tCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAata                            >  1:2428616/1‑93 (MQ=255)

                                                              cAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAatat                           <  2:327474/93‑1 (MQ=255)

                                                              cAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAatat                           >  1:1852665/1‑93 (MQ=255)

                                                              cAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAatat                           <  1:1505668/93‑1 (MQ=255)

                                                              cAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAatat                           <  2:5936710/93‑1 (MQ=255)

                                                               aaaGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATc                          <  2:876840/93‑1 (MQ=255)

                                                               aaaGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATc                          >  1:5766822/1‑93 (MQ=255)

                                                                aaGAGCTTCGCTGGTCATGGGTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGTCAGCGGGGGAGTGCGGGAACAAAAATATCg                         <  2:1269322/93‑1 (MQ=255)

                                                                aaGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCg                         <  2:2933915/93‑1 (MQ=255)

                                                                aaGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCg                         >  1:3543979/1‑93 (MQ=255)

                                                                aaGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCg                         >  2:2187549/1‑93 (MQ=255)

                                                                 agagCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCgg                        <  2:2541028/93‑1 (MQ=255)

                                                                  gagCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGt                       >  1:1470495/1‑93 (MQ=255)

                                                                  gagCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGt                       >  2:2053224/1‑93 (MQ=255)

                                                                   agCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTc                      >  2:3150965/1‑93 (MQ=255)

                                                                    gCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCa                     <  1:3074296/93‑1 (MQ=255)

                                                                     cATCCCTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCaa                    <  2:2212248/93‑1 (MQ=255)

                                                                     cATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCaa                    <  1:568009/93‑1 (MQ=255)

                                                                      aTCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCaaa                   <  1:5085228/93‑1 (MQ=255)

                                                                      aTCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCaaa                   >  2:3209364/1‑93 (MQ=255)

                                                                       tCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAg                  <  2:6028072/93‑1 (MQ=255)

                                                                        cACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGc                 >  1:560725/1‑93 (MQ=255)

                                                                         aCTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGcc                >  2:5535133/1‑93 (MQ=255)

                                                                          cTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCg               >  2:754265/1‑93 (MQ=255)

                                                                           tGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCgg              >  1:4327226/1‑93 (MQ=255)

                                                                            ggTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGa             <  1:4998013/93‑1 (MQ=255)

                                                                            ggTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGa             <  2:1785845/93‑1 (MQ=255)

                                                                             gTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGAt            >  1:6223527/1‑93 (MQ=255)

                                                                               cAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATta          <  1:3270129/93‑1 (MQ=255)

                                                                               cAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATta          <  2:2614842/93‑1 (MQ=255)

                                                                               cAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATta          <  1:5167495/93‑1 (MQ=255)

                                                                                 aCGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATtata        <  2:1960414/93‑1 (MQ=255)

                                                                                 aCGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATtata        >  2:6139496/1‑93 (MQ=255)

                                                                                  cGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAg       <  1:249269/93‑1 (MQ=255)

                                                                                   tttgcttacCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAGa      <  2:462481/92‑1 (MQ=255)

                                                                                   gTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAGa      >  1:341739/1‑93 (MQ=255)

                                                                                   gTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAGa      <  1:1603075/93‑1 (MQ=255)

                                                                                      gcttacCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAGATcc   <  1:1222084/93‑1 (MQ=255)

                                                                                      gcttacCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAGATcc   <  1:3634637/93‑1 (MQ=255)

GCACAGGCATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTGCCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAGATCCC  >  NC_000913/657724‑657903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGGCACAGGCATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTGCCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAGATCCCTTTG  >  NC_000913/657722‑657907

TGGCACAGGCATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTAC                                                                                              >  SRR4302533.3062890/1‑94 (MQ=60)

TGGCACAGGCATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTAC                                                                                              <  SRR4302533.4233825/94‑1 (MQ=60)

TGGCACAGGCATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTAC                                                                                              <  SRR4302533.5793340/94‑1 (MQ=60)

TGGCACAGGCATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTAC                                                                                              >  SRR4302533.5937249/1‑94 (MQ=60)

TGGCACAGGCATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTAC                                                                                              <  SRR4302533.131938/94‑1 (MQ=60)

 GGCACAGGCATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACC                                                                                             >  SRR4302533.3133967/1‑94 (MQ=60)

 GGCACAGGCATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACC                                                                                             <  SRR4302533.6079285/94‑1 (MQ=60)

   CACAGGCATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGC                                                                                           >  SRR4302533.3528061/1‑94 (MQ=60)

     CAGGCATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTC                                                                                         >  SRR4302533.26106/1‑94 (MQ=60)

         CATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGC                                                                                     >  SRR4302533.2323638/1‑94 (MQ=60)

         CATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGC                                                                                     >  SRR4302533.2397926/1‑94 (MQ=60)

          ATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCG                                                                                    >  SRR4302533.811792/1‑94 (MQ=60)

          ATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCG                                                                                    >  SRR4302533.260401/1‑94 (MQ=60)

             ACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTG                                                                                 >  SRR4302533.6265242/1‑94 (MQ=60)

              CAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGG                                                                                <  SRR4302533.6115646/94‑1 (MQ=60)

               AGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGA                                                                               >  SRR4302533.2556671/1‑94 (MQ=60)

               AGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGA                                                                               <  SRR4302533.1696610/94‑1 (MQ=60)

                ttGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGAT                                                                              <  SRR4302533.1733769/92‑1 (MQ=60)

                GGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGAT                                                                              <  SRR4302533.4676323/94‑1 (MQ=60)

                GGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGAT                                                                              <  SRR4302533.2817762/94‑1 (MQ=60)

                GGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGAT                                                                              >  SRR4302533.1078597/1‑94 (MQ=60)

                   AGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACC                                                                           >  SRR4302533.1349608/1‑94 (MQ=60)

                     CTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCAC                                                                         <  SRR4302533.16149/94‑1 (MQ=60)

                     CTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCAC                                                                         >  SRR4302533.3268571/1‑94 (MQ=60)

                        AAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTG                                                                      >  SRR4302533.5310166/1‑94 (MQ=60)

                        AAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTG                                                                      >  SRR4302533.4543916/1‑94 (MQ=60)

                          ATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTT                                                                    >  SRR4302533.6291842/1‑94 (MQ=60)

                          ATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTT                                                                    <  SRR4302533.886984/94‑1 (MQ=60)

                           TCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTA                                                                   >  SRR4302533.3354774/1‑94 (MQ=60)

                            CCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTAT                                                                  >  SRR4302533.5318141/1‑94 (MQ=60)

                            CCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTAT                                                                  >  SRR4302533.2490672/1‑94 (MQ=60)

                             CTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATA                                                                 <  SRR4302533.6177131/94‑1 (MQ=60)

                               GGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATG                                                               >  SRR4302533.1505797/1‑94 (MQ=60)

                               GGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATG                                                               <  SRR4302533.4206010/94‑1 (MQ=60)

                                GTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGG                                                              <  SRR4302533.5633408/94‑1 (MQ=60)

                                GTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGG                                                              >  SRR4302533.6103570/1‑94 (MQ=60)

                                GTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGG                                                              >  SRR4302533.5129163/1‑94 (MQ=60)

                                  TCTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTG                                                            <  SRR4302533.2320760/94‑1 (MQ=60)

                                  TCTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTG                                                            <  SRR4302533.2319572/94‑1 (MQ=60)

                                  ACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTG                                                            >  SRR4302533.3524115/1‑94 (MQ=60)

                                  ACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTG                                                            <  SRR4302533.4306622/94‑1 (MQ=60)

                                  ACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTG                                                            <  SRR4302533.4004390/94‑1 (MQ=60)

                                  ACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTG                                                            <  SRR4302533.5921374/94‑1 (MQ=60)

                                   CTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTCCCGCTCGTGCGCTGGATACCACCTGTTATATGGTGG                                                           <  SRR4302533.280349/94‑1 (MQ=60)

                                   CTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGG                                                           <  SRR4302533.4921584/94‑1 (MQ=60)

                                     TCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCG                                                         <  SRR4302533.5610698/94‑1 (MQ=60)

                                       CTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGG                                                       >  SRR4302533.3288961/1‑94 (MQ=60)

                                       CTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGC                                                       >  SRR4302533.506144/1‑94 (MQ=60)

                                       CTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGC                                                       <  SRR4302533.2134835/94‑1 (MQ=60)

                                       CTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGC                                                       <  SRR4302533.205480/94‑1 (MQ=60)

                                        TGCCGCCTGGTTTCGGGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCG                                                      <  SRR4302533.4781250/94‑1 (MQ=60)

                                        TGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCG                                                      <  SRR4302533.3554805/94‑1 (MQ=60)

                                        TGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCG                                                      <  SRR4302533.4781252/94‑1 (MQ=60)

                                        TGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCG                                                      <  SRR4302533.5308040/94‑1 (MQ=60)

                                        TCCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCG                                                      <  SRR4302533.1289785/94‑1 (MQ=60)

                                           CGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGG                                                   >  SRR4302533.2862657/1‑94 (MQ=60)

                                            GCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGG                                                  <  SRR4302533.4660974/94‑1 (MQ=60)

                                            GCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGG                                                  <  SRR4302533.758794/94‑1 (MQ=60)

                                             CCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGA                                                 <  SRR4302533.2239695/94‑1 (MQ=60)

                                             CCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGA                                                 <  SRR4302533.6225937/94‑1 (MQ=60)

                                              CTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGGTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAG                                                >  SRR4302533.4467853/1‑94 (MQ=60)

                                              CTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAG                                                <  SRR4302533.5789952/94‑1 (MQ=60)

                                              CTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAG                                                >  SRR4302533.1057731/1‑94 (MQ=60)

                                               TGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGT                                               <  SRR4302533.4561415/94‑1 (MQ=60)

                                               TGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGT                                               <  SRR4302533.894009/94‑1 (MQ=60)

                                               TGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGG                                               >  SRR4302533.6160120/1‑94 (MQ=60)

                                                GGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGgggg                                              >  SRR4302533.3271411/1‑90 (MQ=60)

                                                GGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTG                                              >  SRR4302533.2253190/1‑94 (MQ=60)

                                                GGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTG                                              >  SRR4302533.257083/1‑94 (MQ=60)

                                                 GGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGC                                             <  SRR4302533.2382153/94‑1 (MQ=60)

                                                  GTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCG                                            <  SRR4302533.2199288/94‑1 (MQ=60)

                                                   TTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGG                                           <  SRR4302533.1295536/94‑1 (MQ=60)

                                                    TCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGG                                          <  SRR4302533.3582863/94‑1 (MQ=60)

                                                    TCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGG                                          <  SRR4302533.4868941/94‑1 (MQ=60)

                                                     CGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGA                                         <  SRR4302533.1794479/94‑1 (MQ=60)

                                                     CGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGA                                         <  SRR4302533.5508077/94‑1 (MQ=60)

                                                      GCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAA                                        <  SRR4302533.3276305/94‑1 (MQ=60)

                                                      GCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAA                                        <  SRR4302533.4898416/94‑1 (MQ=60)

                                                        GGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGGGCGGGAACA                                      >  SRR4302533.5524220/1‑94 (MQ=60)

                                                            CGCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAA                                  >  SRR4302533.1431898/1‑94 (MQ=60)

                                                             GCTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAAT                                 <  SRR4302533.2386631/94‑1 (MQ=60)

                                                              CTCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATA                                >  SRR4302533.2443630/1‑94 (MQ=60)

                                                               TCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATAT                               >  SRR4302533.1863961/1‑94 (MQ=60)

                                                               TCAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGGGCGGGAACAAAAATAT                               >  SRR4302533.775300/1‑94 (MQ=60)

                                                                CAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATC                              <  SRR4302533.106895/94‑1 (MQ=60)

                                                                CAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATC                              <  SRR4302533.5573415/94‑1 (MQ=60)

                                                                CAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATC                              <  SRR4302533.1514749/94‑1 (MQ=60)

                                                                CAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATC                              <  SRR4302533.1556147/94‑1 (MQ=60)

                                                                CAAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATC                              >  SRR4302533.5801636/1‑94 (MQ=60)

                                                                 AAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCG                             >  SRR4302533.3565819/1‑94 (MQ=60)

                                                                 AAAGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCG                             >  SRR4302533.5936018/1‑94 (MQ=60)

                                                                   AGAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGT                           >  SRR4302533.1479357/1‑94 (MQ=60)

                                                                    GAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTC                          <  SRR4302533.2305187/94‑1 (MQ=60)

                                                                    GAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTC                          <  SRR4302533.311988/94‑1 (MQ=60)

                                                                    GAGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTC                          <  SRR4302533.588621/94‑1 (MQ=60)

                                                                     AGCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCA                         >  SRR4302533.2356166/1‑94 (MQ=60)

                                                                      GCATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAA                        <  SRR4302533.3093167/94‑1 (MQ=60)

                                                                       CATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAA                       >  SRR4302533.3723428/1‑94 (MQ=60)

                                                                       CATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAA                       <  SRR4302533.571369/94‑1 (MQ=60)

                                                                        ATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAG                      <  SRR4302533.4496744/94‑1 (MQ=60)

                                                                        ATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAG                      <  SRR4302533.5115959/94‑1 (MQ=60)

                                                                        ATCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAG                      <  SRR4302533.2589293/94‑1 (MQ=60)

                                                                         TCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGC                     <  SRR4302533.2838577/94‑1 (MQ=60)

                                                                         TCACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGC                     >  SRR4302533.564052/1‑94 (MQ=60)

                                                                          CACTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCC                    <  SRR4302533.2837938/94‑1 (MQ=60)

                                                                            CTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGG                  >  SRR4302533.1184745/1‑94 (MQ=60)

                                                                            CTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGG                  >  SRR4302533.4353293/1‑94 (MQ=60)

                                                                            CTGGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGG                  <  SRR4302533.3507888/94‑1 (MQ=60)

                                                                              GGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGAT                <  SRR4302533.2725445/94‑1 (MQ=60)

                                                                              GGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGAT                >  SRR4302533.6260824/1‑94 (MQ=60)

                                                                              GGTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGAT                <  SRR4302533.5028313/94‑1 (MQ=60)

                                                                               GTCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATT               <  SRR4302533.4420414/94‑1 (MQ=60)

                                                                                TCAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTA              <  SRR4302533.3017706/94‑1 (MQ=60)

                                                                                 CAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTAT             <  SRR4302533.3290306/94‑1 (MQ=60)

                                                                                 CAACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTAT             <  SRR4302533.5198691/94‑1 (MQ=60)

                                                                                  AACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATA            >  SRR4302533.5658408/1‑94 (MQ=60)

                                                                                  AACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATA            <  SRR4302533.2809844/94‑1 (MQ=60)

                                                                                  AACGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATA            <  SRR4302533.561547/94‑1 (MQ=60)

                                                                                    CGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAGA          <  SRR4302533.250809/94‑1 (MQ=60)

                                                                                    CGTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAGA          >  SRR4302533.343825/1‑94 (MQ=60)

                                                                                     GTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAGAT         >  SRR4302533.5015099/1‑94 (MQ=60)

                                                                                     GTTGCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAGAT         <  SRR4302533.1612781/94‑1 (MQ=60)

                                                                                        GCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAGATCCC      <  SRR4302533.3656991/94‑1 (MQ=60)

                                                                                        GCTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAGATCCC      <  SRR4302533.1229413/94‑1 (MQ=60)

                                                                                         CTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAGATCCCT     >  SRR4302533.61568/1‑94 (MQ=60)

                                                                                         CTTACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAGATCCCT     >  SRR4302533.3910556/1‑94 (MQ=60)

                                                                                           TACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAGATCCCTTT   <  SRR4302533.3907713/94‑1 (MQ=60)

                                                                                            ACCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAGATCCCTTTG  >  SRR4302533.2304414/1‑94 (MQ=60)

TGGCACAGGCATTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGCGGGCCGCTCAAAGAGCATCACTGGTCAACGTTGCTTGCCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATATCGGTCAAAGCCGGATTATAGATCCCTTTG  >  NC_000913/657722‑657907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`