Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	667,973	C→T	R96H (CGC→CAC)	ybeB ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	667,973	0	C	T	99.7%	1136.6 / NA	317	R96H (CGC→CAC)	ybeB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (159/157); minor base G (1/0); total (160/157)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 321 total aligned reads displayed.

CCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGC  >  NC_000913/667883‑668062

ccGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGa                                                                                         <  1:7286447/93‑1 (MQ=255)

ccGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGa                                                                                         <  2:1223709/93‑1 (MQ=255)

ccGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGa                                                                                         <  1:8872995/93‑1 (MQ=255)

  ggTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACg                                                                                       >  1:2164475/1‑93 (MQ=255)

   gTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACgg                                                                                      <  2:9618005/93‑1 (MQ=255)

      tGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGctc                                                                                   <  2:840587/93‑1 (MQ=255)

        tACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGctctc                                                                                 <  1:3546330/93‑1 (MQ=255)

           ccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTc                                                                              <  1:4959020/93‑1 (MQ=255)

            cAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTcc                                                                             <  2:8807485/93‑1 (MQ=255)

            cAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTcc                                                                             <  2:8141343/93‑1 (MQ=255)

              gTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTg                                                                           >  2:9710564/1‑93 (MQ=255)

              gTCCGGCATTTTCATTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTg                                                                           >  1:8536657/1‑93 (MQ=255)

               tCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGc                                                                          >  2:3462760/1‑93 (MQ=255)

               tCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGc                                                                          >  2:5645777/1‑93 (MQ=255)

               tCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGc                                                                          >  2:5954136/1‑93 (MQ=255)

               tCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGc                                                                          <  1:5043531/93‑1 (MQ=255)

                ccGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCa                                                                         >  1:3056197/1‑93 (MQ=255)

                ccGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCa                                                                         <  2:1428284/93‑1 (MQ=255)

                   gCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGa                                                                      >  2:9127321/1‑93 (MQ=255)

                   gCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGa                                                                      >  1:8303009/1‑93 (MQ=255)

                    cATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGAc                                                                     >  1:1632199/1‑93 (MQ=255)

                     aTTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACa                                                                    <  2:1006837/93‑1 (MQ=255)

                     aTTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACa                                                                    >  2:4921596/1‑93 (MQ=255)

                      ttttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACAt                                                                   >  2:5255530/1‑93 (MQ=255)

                      ttttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACAt                                                                   >  2:2024871/1‑93 (MQ=255)

                      ttttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACAt                                                                   >  2:3627307/1‑93 (MQ=255)

                      ttttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATAAAGGTGACGGCTCTCTTCCTGCATGACAt                                                                   <  2:574903/93‑1 (MQ=255)

                       tttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCCGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATg                                                                  >  1:5993281/1‑93 (MQ=255)

                          cGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGAc                                                               <  1:6871566/93‑1 (MQ=255)

                          cGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGAc                                                               <  2:5517814/93‑1 (MQ=255)

                          cGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGAc                                                               >  2:2523614/1‑93 (MQ=255)

                            ttCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACaa                                                             >  1:762537/1‑93 (MQ=255)

                            ttCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACaa                                                             <  2:723962/93‑1 (MQ=255)

                             tCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAAt                                                            >  2:6402785/1‑93 (MQ=255)

                              cccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATc                                                           <  2:1772590/93‑1 (MQ=255)

                               ccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATca                                                          <  2:8553200/93‑1 (MQ=255)

                               ccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATca                                                          >  1:998601/1‑93 (MQ=255)

                                  cGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACAt                                                       >  2:6556204/1‑93 (MQ=255)

                                      gACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGcc                                                   >  1:9330772/1‑93 (MQ=255)

                                      gACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGcc                                                   >  1:272397/1‑93 (MQ=255)

                                        cAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCa                                                 >  1:4310096/1‑93 (MQ=255)

                                         aaGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCaa                                                >  2:7076618/1‑93 (MQ=255)

                                         aaGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCaa                                                >  1:4450018/1‑93 (MQ=255)

                                           gTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAAt                                              >  2:5354929/1‑93 (MQ=255)

                                             tGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATcc                                            >  1:4116980/1‑93 (MQ=255)

                                               cAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCAc                                          <  2:2853605/93‑1 (MQ=255)

                                                aGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACg                                         <  1:9763314/93‑1 (MQ=255)

                                                 gCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGa                                        <  1:7768557/93‑1 (MQ=255)

                                                 gCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGa                                        >  1:6240280/1‑93 (MQ=255)

                                                 gCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGa                                        <  2:2237622/93‑1 (MQ=255)

                                                  cTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGAc                                       <  1:750747/93‑1 (MQ=255)

                                                  cTTCACGCAGTAACTCCTGAGTTTTTCCATTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGAc                                       <  2:2307612/93‑1 (MQ=255)

                                                   ttCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACa                                      >  2:2680762/1‑93 (MQ=255)

                                                     cACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAAt                                    >  1:3883812/1‑93 (MQ=255)

                                                     cACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAAt                                    >  2:3375435/1‑93 (MQ=255)

                                                       cGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATcc                                  <  1:835975/93‑1 (MQ=255)

                                                       cGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATcc                                  <  1:1178088/93‑1 (MQ=255)

                                                        gCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCa                                 >  2:9844441/1‑93 (MQ=255)

                                                         cATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAg                                <  1:6489052/93‑1 (MQ=255)

                                                          aTTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGt                               >  2:3190873/1‑93 (MQ=255)

                                                          aTTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGt                               >  2:3955111/1‑93 (MQ=255)

                                                           ttAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCa                                           <  2:3985368/80‑1 (MQ=255)

                                                           ttAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTc                              >  1:8031387/1‑93 (MQ=255)

                                                           ttAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTc                              <  2:1951735/93‑1 (MQ=255)

                                                            tAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCg                             >  2:4853392/1‑93 (MQ=255)

                                                            tAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCg                             >  1:2292304/1‑93 (MQ=255)

                                                             aaCTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCgg                            <  2:8024186/93‑1 (MQ=255)

                                                             aaCTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCgg                            <  2:2743148/93‑1 (MQ=255)

                                                              aCTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGgc                           >  1:4692649/1‑93 (MQ=255)

                                                              aCTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGgc                           <  1:5230309/93‑1 (MQ=255)

                                                               cTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGgcg                          >  2:7634824/1‑93 (MQ=255)

                                                               cTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGgcg                          >  2:5051484/1‑93 (MQ=255)

                                                                 ccAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCt                        >  1:9858607/1‑93 (MQ=255)

                                                                 ccAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCt                        <  1:4545393/93‑1 (MQ=255)

                                                                 ccAGAGTTTTTCCAGTACATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCt                        >  1:3717690/1‑93 (MQ=255)

                                                                   agagTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGt                      <  2:258762/93‑1 (MQ=255)

                                                                   agagTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGt                      >  1:1459875/1‑93 (MQ=255)

                                                                    gagTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGtt                     >  2:8524013/1‑93 (MQ=255)

                                                                      gTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGtttt                   <  2:5458735/93‑1 (MQ=255)

                                                                       tttttCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTc                  >  1:3351224/1‑93 (MQ=255)

                                                                        ttttCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCa                 <  2:7901334/93‑1 (MQ=255)

                                                                         tttCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATTCAGTCGGCGCTGTTTTCAc                <  1:9082354/93‑1 (MQ=255)

                                                                          ttCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCAcc               <  1:1751477/93‑1 (MQ=255)

                                                                          ttCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCAcc               >  2:3791301/1‑93 (MQ=255)

                                                                            ccAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCtt             <  1:6967403/93‑1 (MQ=255)

                                                                            ccAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCtt             >  1:9254755/1‑93 (MQ=255)

                                                                            ccAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCtt             >  1:2063192/1‑93 (MQ=255)

                                                                              aGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCt           <  2:5851521/93‑1 (MQ=255)

                                                                               gTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTa          <  1:5714469/93‑1 (MQ=255)

                                                                               gTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTa          >  1:4400633/1‑93 (MQ=255)

                                                                               gTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTa          >  2:6816043/1‑93 (MQ=255)

                                                                               gTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTa          >  2:4448137/1‑93 (MQ=255)

                                                                                ttCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTAc         <  2:4240874/93‑1 (MQ=255)

                                                                                 tCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACg        >  2:4686324/1‑93 (MQ=255)

                                                                                   aTACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGcc      <  2:7400859/93‑1 (MQ=255)

                                                                                   aTACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGcc      <  1:6815416/93‑1 (MQ=255)

                                                                                    tACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGcct     >  2:6038566/1‑92 (MQ=255)

                                                                                    tACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCg     <  1:420150/93‑1 (MQ=255)

                                                                                    tACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCg     >  2:9223744/1‑93 (MQ=255)

                                                                                       aGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGc  >  1:1006430/1‑93 (MQ=255)

CCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGC  >  NC_000913/667883‑668062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTA  >  NC_000913/667880‑668066

AAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGT                                                                                               <  SRR4302540.9740975/94‑1 (MQ=60)

AAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGT                                                                                               <  SRR4302540.2387379/94‑1 (MQ=60)

AAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGG                                                                                               >  SRR4302540.5072899/1‑94 (MQ=60)

 AACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTG                                                                                              >  SRR4302540.6806023/1‑94 (MQ=60)

 AACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTG                                                                                              <  SRR4302540.1477864/94‑1 (MQ=60)

  ACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGA                                                                                             <  SRR4302540.8696703/94‑1 (MQ=60)

   CCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGAC                                                                                            >  SRR4302540.4221985/1‑94 (MQ=60)

   CCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGAC                                                                                            <  SRR4302540.7367751/94‑1 (MQ=60)

   CCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGAC                                                                                            <  SRR4302540.6897538/94‑1 (MQ=60)

   CCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGAC                                                                                            <  SRR4302540.8975684/94‑1 (MQ=60)

    CGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACG                                                                                           >  SRR4302540.5215164/1‑94 (MQ=60)

    CGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACG                                                                                           <  SRR4302540.4221073/94‑1 (MQ=60)

    CGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACG                                                                                           >  SRR4302540.2187789/1‑94 (MQ=60)

    CGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACG                                                                                           <  SRR4302540.4221072/94‑1 (MQ=60)

     GGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGG                                                                                          <  SRR4302540.6241431/94‑1 (MQ=60)

     GGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGG                                                                                          >  SRR4302540.2147095/1‑94 (MQ=60)

        TTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTC                                                                                       >  SRR4302540.7513302/1‑94 (MQ=60)

        TTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTC                                                                                       <  SRR4302540.7816672/94‑1 (MQ=60)

         TGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCT                                                                                      <  SRR4302540.5649952/94‑1 (MQ=60)

         TGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCT                                                                                      <  SRR4302540.6046252/94‑1 (MQ=60)

          GTACCCAGTCCGGCATTTTCGTTCTCACGGCGACAAGTTGCAGCTTCATGCATTAACTCCAGAGTGTTTCTAGTACATACAGGTGACGGCTCTC                                                                                     >  SRR4302540.9070757/1‑94 (MQ=60)

           TACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCT                                                                                    <  SRR4302540.3585059/94‑1 (MQ=60)

           TACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCT                                                                                    <  SRR4302540.9414246/94‑1 (MQ=60)

             CCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTC                                                                                  <  SRR4302540.1286290/94‑1 (MQ=60)

              CCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCC                                                                                 <  SRR4302540.5012626/94‑1 (MQ=60)

              CCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCC                                                                                 >  SRR4302540.8257682/1‑94 (MQ=60)

               CAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCT                                                                                <  SRR4302540.6755450/94‑1 (MQ=60)

                cgtgCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTG                                                                               <  SRR4302540.7986667/90‑1 (MQ=60)

                AGTCCGGCATTTTCATTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTG                                                                               >  SRR4302540.8635854/1‑94 (MQ=60)

                 GTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGC                                                                              >  SRR4302540.9962139/1‑94 (MQ=60)

                  TCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCA                                                                             <  SRR4302540.5098086/94‑1 (MQ=60)

                  TCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCA                                                                             >  SRR4302540.3089725/1‑94 (MQ=60)

                  TCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCA                                                                             >  SRR4302540.3412727/1‑94 (MQ=60)

                     GGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGA                                                                          <  SRR4302540.8573851/94‑1 (MQ=60)

                     GGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGA                                                                          >  SRR4302540.9273024/1‑94 (MQ=60)

                     GGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGA                                                                          >  SRR4302540.8399420/1‑94 (MQ=60)

                      GCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGAC                                                                         >  SRR4302540.1650058/1‑94 (MQ=60)

                       GATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACA                                                                        <  SRR4302540.8786806/94‑1 (MQ=60)

                       GATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACA                                                                        <  SRR4302540.4418287/94‑1 (MQ=60)

                       CATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACA                                                                        <  SRR4302540.6252696/94‑1 (MQ=60)

                        ATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACAT                                                                       >  SRR4302540.4589103/1‑94 (MQ=60)

                        ATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACAT                                                                       <  SRR4302540.4466390/94‑1 (MQ=60)

                        ATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACAT                                                                       <  SRR4302540.1588858/94‑1 (MQ=60)

                        ATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACAT                                                                       >  SRR4302540.5057822/1‑94 (MQ=60)

                         TTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCCGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATG                                                                      >  SRR4302540.6059676/1‑94 (MQ=60)

                         TTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATG                                                                      <  SRR4302540.1960655/94‑1 (MQ=60)

                             CGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACA                                                                  <  SRR4302540.2181251/94‑1 (MQ=60)

                             CGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACA                                                                  <  SRR4302540.6947730/94‑1 (MQ=60)

                              GTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAA                                                                 <  SRR4302540.6907818/94‑1 (MQ=60)

                              GTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAA                                                                 >  SRR4302540.770652/1‑94 (MQ=60)

                              GTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAA                                                                 >  SRR4302540.7650989/1‑94 (MQ=60)

                              GTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAA                                                                 <  SRR4302540.3076435/94‑1 (MQ=60)

                               TTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAAT                                                                <  SRR4302540.1169216/94‑1 (MQ=60)

                                 CCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCA                                                              <  SRR4302540.8564681/94‑1 (MQ=60)

                                 CCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCA                                                              >  SRR4302540.1008751/1‑94 (MQ=60)

                                   CACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACA                                                            >  SRR4302540.165694/1‑94 (MQ=60)

                                   CACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACA                                                            >  SRR4302540.3835232/1‑94 (MQ=60)

                                   CACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACA                                                            >  SRR4302540.1918999/1‑94 (MQ=60)

                                    ACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACAT                                                           <  SRR4302540.4402847/94‑1 (MQ=60)

                                        CGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCC                                                       >  SRR4302540.9442026/1‑94 (MQ=60)

                                        CGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCC                                                       >  SRR4302540.275079/1‑94 (MQ=60)

                                         GACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCC                                                      >  SRR4302540.4730846/1‑94 (MQ=60)

                                         GACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCC                                                      >  SRR4302540.228179/1‑94 (MQ=60)

                                          ACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCA                                                     >  SRR4302540.4357014/1‑94 (MQ=60)

                                          ACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCA                                                     <  SRR4302540.3344610/94‑1 (MQ=60)

                                          ACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCA                                                     >  SRR4302540.7007100/1‑94 (MQ=60)

                                          ACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCA                                                     <  SRR4302540.8772506/94‑1 (MQ=60)

                                           CAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAA                                                    >  SRR4302540.4498249/1‑94 (MQ=60)

                                            AAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGATATGGACAATCACATCGCCCAAT                                                   >  SRR4302540.9164136/1‑94 (MQ=60)

                                            AAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAA                                                   >  SRR4302540.6405819/1‑94 (MQ=60)

                                             AGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAAT                                                  >  SRR4302540.2267640/1‑94 (MQ=60)

                                              GTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATC                                                 <  SRR4302540.1689185/94‑1 (MQ=60)

                                              GTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATC                                                 >  SRR4302540.7337442/1‑94 (MQ=60)

                                               TTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCC                                                <  SRR4302540.4689429/94‑1 (MQ=60)

                                               TTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCC                                                >  SRR4302540.4161433/1‑94 (MQ=60)

                                                 GCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCAC                                              <  SRR4302540.7402983/94‑1 (MQ=60)

                                                 GCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCAC                                              <  SRR4302540.1456116/94‑1 (MQ=60)

                                                   AGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGTCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGA                                            >  SRR4302540.6973149/1‑94 (MQ=60)

                                                   AGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGA                                            <  SRR4302540.9877820/94‑1 (MQ=60)

                                                   AGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGA                                            >  SRR4302540.6309720/1‑94 (MQ=60)

                                                    GCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGAC                                           <  SRR4302540.7859379/94‑1 (MQ=60)

                                                    GCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGAC                                           <  SRR4302540.7859100/94‑1 (MQ=60)

                                                     CTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACA                                          <  SRR4302540.758739/94‑1 (MQ=60)

                                                     CTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACA                                          <  SRR4302540.758738/94‑1 (MQ=60)

                                                     CTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACA                                          >  SRR4302540.918355/1‑94 (MQ=60)

                                                     CTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACAACA                                          >  SRR4302540.6319347/1‑94 (MQ=60)

                                                      TTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAA                                         >  SRR4302540.9975730/1‑94 (MQ=60)

                                                       TCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAAT                                        >  SRR4302540.3926007/1‑94 (MQ=60)

                                                       TCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAAT                                        <  SRR4302540.3169903/94‑1 (MQ=60)

                                                          CGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCA                                     <  SRR4302540.1190264/94‑1 (MQ=60)

                                                          CGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCA                                     <  SRR4302540.3704728/94‑1 (MQ=60)

                                                          CGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCA                                     <  SRR4302540.4468811/94‑1 (MQ=60)

                                                          CGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCA                                     <  SRR4302540.844851/94‑1 (MQ=60)

                                                          CGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCA                                     <  SRR4302540.844850/94‑1 (MQ=60)

                                                            CATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGT                                   <  SRR4302540.6561214/94‑1 (MQ=60)

                                                             ATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACaagcgctagatc                                  >  SRR4302540.4028282/1‑82 (MQ=60)

                                                             ATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTC                                  >  SRR4302540.8124758/1‑94 (MQ=60)

                                                             ATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTC                                  >  SRR4302540.2438675/1‑94 (MQ=60)

                                                             ATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTC                                  >  SRR4302540.4771237/1‑94 (MQ=60)

                                                             ATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTC                                  <  SRR4302540.3563585/94‑1 (MQ=60)

                                                              TTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCG                                 <  SRR4302540.309082/94‑1 (MQ=60)

                                                              TTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCG                                 <  SRR4302540.6895980/94‑1 (MQ=60)

                                                              TTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCG                                 >  SRR4302540.4527959/1‑94 (MQ=60)

                                                              TTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCG                                 >  SRR4302540.2316973/1‑94 (MQ=60)

                                                                AACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGC                               >  SRR4302540.4742819/1‑94 (MQ=60)

                                                                 ACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCG                              <  SRR4302540.5287348/94‑1 (MQ=60)

                                                                 ACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCG                              <  SRR4302540.5287347/94‑1 (MQ=60)

                                                                  CTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGC                             >  SRR4302540.4665890/1‑94 (MQ=60)

                                                                  CTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGC                             >  SRR4302540.3763472/1‑94 (MQ=60)

                                                                   TCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCT                            >  SRR4302540.9501494/1‑94 (MQ=60)

                                                                   TCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCT                            >  SRR4302540.9973805/1‑94 (MQ=60)

                                                                   TCCAGAGTTTTTCCAGTACATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCT                            >  SRR4302540.3758252/1‑94 (MQ=60)

                                                                    CCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTG                           <  SRR4302540.5055514/94‑1 (MQ=60)

                                                                    CCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTG                           <  SRR4302540.4594578/94‑1 (MQ=60)

                                                                     CAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGT                          >  SRR4302540.1475769/1‑94 (MQ=60)

                                                                     CAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGT                          >  SRR4302540.5754474/1‑94 (MQ=60)

                                                                       GAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTT                        <  SRR4302540.6606437/94‑1 (MQ=60)

                                                                        AGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTT                       >  SRR4302540.5603118/1‑94 (MQ=60)

                                                                        AGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTT                       <  SRR4302540.9912189/94‑1 (MQ=60)

                                                                         GTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTC                      >  SRR4302540.3387960/1‑94 (MQ=60)

                                                                          TTTTTCCGTTTTATACAGGCTACTGATCTCTTCCTGGATGACATTGACAATCACATCGCCCAAATCCACGACAATCCAGGCGGCGCTGTTTTCA                     <  SRR4302540.7295758/94‑1 (MQ=60)

                                                                          TTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCA                     >  SRR4302540.9406530/1‑94 (MQ=60)

                                                                           TTTTCCAGCTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCAC                    >  SRR4302540.4012429/1‑94 (MQ=60)

                                                                            TTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATTCAGTCGGCGCTGTTTTCACC                   <  SRR4302540.9191469/94‑1 (MQ=60)

                                                                             TTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCT                  <  SRR4302540.8267559/94‑1 (MQ=60)

                                                                             TTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCT                  <  SRR4302540.1770452/94‑1 (MQ=60)

                                                                              TCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCAGCTT                 >  SRR4302540.4916721/1‑94 (MQ=60)

                                                                              TCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTT                 >  SRR4302540.9365511/1‑94 (MQ=60)

                                                                              TCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTT                 >  SRR4302540.2085392/1‑94 (MQ=60)

                                                                               CCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTC                <  SRR4302540.7044868/94‑1 (MQ=60)

                                                                               CCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTC                >  SRR4302540.9775636/1‑94 (MQ=60)

                                                                               CCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTC                >  SRR4302540.2707685/1‑94 (MQ=60)

                                                                               CCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTC                >  SRR4302540.1993856/1‑94 (MQ=60)

                                                                                 AGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTA              >  SRR4302540.1486307/1‑94 (MQ=60)

                                                                                 AGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTA              >  SRR4302540.4448358/1‑94 (MQ=60)

                                                                                 AGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTA              <  SRR4302540.362436/94‑1 (MQ=60)

                                                                                  GTTCATACAGGTGACGGCTCTCTTCGTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTAC             <  SRR4302540.1030486/94‑1 (MQ=60)

                                                                                  GTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTAC             <  SRR4302540.5777371/94‑1 (MQ=60)

                                                                                   TTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACG            <  SRR4302540.4263744/94‑1 (MQ=60)

                                                                                   TTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACG            >  SRR4302540.6023623/1‑94 (MQ=60)

                                                                                   TTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACG            >  SRR4302540.8413761/1‑94 (MQ=60)

                                                                                    TCATACAGGTGACGGCTCTCTTCCTGCATGATATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGC           >  SRR4302540.9550949/1‑94 (MQ=60)

                                                                                    TCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGC           >  SRR4302540.8032930/1‑94 (MQ=60)

                                                                                     CATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCC          >  SRR4302540.7757374/1‑94 (MQ=60)

                                                                                     CATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCC          >  SRR4302540.4346728/1‑94 (MQ=60)

                                                                                      ATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCG         >  SRR4302540.10008401/1‑94 (MQ=60)

                                                                                      ATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCG         <  SRR4302540.6890853/94‑1 (MQ=60)

                                                                                      ATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGGTTTCACCTTCTAagacg         >  SRR4302540.7280544/1‑89 (MQ=60)

                                                                                       TACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGA        <  SRR4302540.424561/94‑1 (MQ=60)

                                                                                         CAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGC      >  SRR4302540.1016636/1‑94 (MQ=60)

                                                                                         CAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGC      <  SRR4302540.284472/94‑1 (MQ=60)

                                                                                          AGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCG     <  SRR4302540.8858002/94‑1 (MQ=60)

                                                                                           GGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGG    >  SRR4302540.7010330/1‑94 (MQ=60)

                                                                                             TGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTA  >  SRR4302540.2276825/1‑94 (MQ=60)

AAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTA  >  NC_000913/667880‑668066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`