Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	667,973	C→T	R96H (CGC→CAC)	ybeB ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	667,973	0	C	T	99.6%	848.9 / NA	234	R96H (CGC→CAC)	ybeB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (130/103); minor base G (1/0); total (131/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

CCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGC  >  NC_000913/667883‑668062

ccGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGa                                                                                         >  1:1954118/1‑93 (MQ=255)

   gTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACgg                                                                                      <  2:3793195/93‑1 (MQ=255)

    tttGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGc                                                                                     <  2:870214/93‑1 (MQ=255)

    tttGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGc                                                                                     >  2:242664/1‑93 (MQ=255)

          cccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCtt                                                                               <  1:514351/93‑1 (MQ=255)

          cccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCtt                                                                               >  2:2547794/1‑93 (MQ=255)

            cAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTcc                                                                             >  2:5077695/1‑93 (MQ=255)

            cAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTcc                                                                             <  2:5718037/93‑1 (MQ=255)

            cAGTCCGGCATTTTCGTGCCCACGGCGACAAGTTGCAGCTTCACGCATTAAGTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTcc                                                                             <  2:4169794/93‑1 (MQ=255)

             aGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCt                                                                            <  1:1049995/93‑1 (MQ=255)

             aGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCt                                                                            >  2:5195834/1‑93 (MQ=255)

             aGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCt                                                                            <  1:3241154/93‑1 (MQ=255)

              gTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTg                                                                           >  1:650031/1‑93 (MQ=255)

               tCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGc                                                                          >  1:5716831/1‑93 (MQ=255)

                ccGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCa                                                                         >  2:4731315/1‑93 (MQ=255)

                 cGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCAt                                                                        >  1:4835602/1‑93 (MQ=255)

                  ggCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATg                                                                       >  2:6129301/1‑93 (MQ=255)

                     aTTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACa                                                                    >  2:3614502/1‑93 (MQ=255)

                     aTTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACa                                                                    >  2:674664/1‑93 (MQ=255)

                      ttttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACAt                                                                   <  1:4992228/93‑1 (MQ=255)

                      ttttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACAt                                                                   >  1:4161797/1‑93 (MQ=255)

                        ttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATgg                                                                 <  2:1492818/93‑1 (MQ=255)

                         tCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGa                                                                <  2:2178618/93‑1 (MQ=255)

                         tCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGa                                                                >  1:5885123/1‑93 (MQ=255)

                          cGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTTCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGAc                                                               <  1:178798/93‑1 (MQ=255)

                          cGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGAc                                                               <  1:4678805/93‑1 (MQ=255)

                            ttCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACaa                                                             >  1:705629/1‑93 (MQ=255)

                             tCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAAt                                                            <  1:2418360/93‑1 (MQ=255)

                             tCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAAt                                                            >  1:283391/1‑93 (MQ=255)

                              cccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATc                                                           >  2:3986654/1‑93 (MQ=255)

                              cccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATc                                                           >  1:258792/1‑93 (MQ=255)

                               cgaCGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATca                                                          >  2:6031602/3‑93 (MQ=255)

                               ccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATca                                                          >  2:1936675/1‑93 (MQ=255)

                               ccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATca                                                          >  1:5446820/1‑93 (MQ=255)

                                cACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATcac                                                         <  1:2781176/93‑1 (MQ=255)

                                 aCGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATcaca                                                        <  2:775785/93‑1 (MQ=255)

                                  cGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACAt                                                       <  1:2919013/93‑1 (MQ=255)

                                  cGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACAt                                                       <  1:6177000/93‑1 (MQ=255)

                                   ggCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATc                                                      <  2:1916335/93‑1 (MQ=255)

                                     cGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGc                                                    <  1:2174445/93‑1 (MQ=255)

                                      gACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGcc                                                   >  2:545252/1‑93 (MQ=255)

                                      gACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGcc                                                   >  2:5146351/1‑93 (MQ=255)

                                      gACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGcc                                                   >  1:1410114/1‑93 (MQ=255)

                                      gACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGcc                                                   >  2:4377576/1‑93 (MQ=255)

                                      gACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGATCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGcc                                                   >  1:4437415/1‑93 (MQ=255)

                                       aCAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGccc                                                  >  2:469954/1‑93 (MQ=255)

                                       aCAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGccc                                                  >  2:4491134/1‑93 (MQ=255)

                                          aGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCaaa                                               <  1:1937896/93‑1 (MQ=255)

                                          aGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCaaa                                               <  2:4782755/93‑1 (MQ=255)

                                          aGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCaaa                                               >  2:3183327/1‑93 (MQ=255)

                                            ttGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATc                                             >  2:5307991/1‑93 (MQ=255)

                                               cAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCAc                                          <  1:2015219/93‑1 (MQ=255)

                                                 gCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGa                                        <  1:6148835/93‑1 (MQ=255)

                                                 gCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGa                                        <  2:1190410/93‑1 (MQ=255)

                                                 gCTTCACGCAGTAACTCGAGAGTTTTTCCAGGTCATACAGGTGACGGCTCTATTCCTGCATGACATGCAAAATCACATCGCCCAAATCCACGa                                        >  2:3417503/1‑93 (MQ=255)

                                                  cTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGAc                                       <  2:901323/93‑1 (MQ=255)

                                                   ttCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACa                                      <  1:5796322/93‑1 (MQ=255)

                                                    tCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACaa                                     <  1:4203343/93‑1 (MQ=255)

                                                    tCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACaa                                     <  1:3453292/93‑1 (MQ=255)

                                                     cACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAAt                                    >  1:2476775/1‑93 (MQ=255)

                                                     cACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAAt                                    >  2:3341348/1‑93 (MQ=255)

                                                       cGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATcc                                  <  1:1726852/93‑1 (MQ=255)

                                                        gCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCa                                 <  1:3341554/93‑1 (MQ=255)

                                                        gCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCa                                 <  1:5844421/93‑1 (MQ=255)

                                                         cATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAg                                >  1:877826/1‑93 (MQ=255)

                                                         cATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAg                                >  2:5840438/1‑93 (MQ=255)

                                                         cATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAg                                <  1:2626894/93‑1 (MQ=255)

                                                          aTTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCGCATCGCCCAAATCCACGACAATCCAGt                               <  2:3066004/93‑1 (MQ=255)

                                                          aTTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGt                               >  1:1547052/1‑93 (MQ=255)

                                                           ttAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCGTGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTc                              >  1:3610973/1‑93 (MQ=255)

                                                           ttAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTc                              >  1:5552273/1‑93 (MQ=255)

                                                           ttAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTc                              <  1:1480213/93‑1 (MQ=255)

                                                            tAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCg                             >  2:4420503/1‑93 (MQ=255)

                                                             aaCTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCgg                            >  1:1080070/1‑93 (MQ=255)

                                                              aCTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGgc                           >  1:4890552/1‑93 (MQ=255)

                                                               cTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGgcg                          <  2:1962638/93‑1 (MQ=255)

                                                                  cAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTg                       >  1:6040784/1‑93 (MQ=255)

                                                                  cAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTg                       >  2:3492046/1‑93 (MQ=255)

                                                                   agagTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGt                      >  1:2219995/1‑93 (MQ=255)

                                                                    gagTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGtt                     >  2:1696083/1‑93 (MQ=255)

                                                                    gagTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGtt                     >  1:799737/1‑93 (MQ=255)

                                                                    gagTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGtt                     >  2:4932348/1‑93 (MQ=255)

                                                                      gTTTTTCCAGTTCATACAGGGGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGtttt                   >  1:3131844/1‑93 (MQ=255)

                                                                       tttttCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTc                  <  1:2511277/93‑1 (MQ=255)

                                                                       tttttCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTc                  >  2:5566958/1‑93 (MQ=255)

                                                                        ttttCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCa                 <  1:5975667/93‑1 (MQ=255)

                                                                        ttttCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCa                 <  1:4515625/93‑1 (MQ=255)

                                                                           tCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCt              >  1:4380009/1‑93 (MQ=255)

                                                                           tCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCt              <  2:545919/93‑1 (MQ=255)

                                                                             cAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCttt            >  2:1315344/1‑92 (MQ=255)

                                                                              aGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCt           <  1:256287/93‑1 (MQ=255)

                                                                              aGTTCATACAGGTGACGGCTCTCTTCCTGCAAGACATGAACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCt           >  2:2792717/1‑93 (MQ=255)

                                                                                 tCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACg        <  2:3968870/93‑1 (MQ=255)

                                                                                   aTACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGcc      >  1:1350127/1‑93 (MQ=255)

                                                                                    tACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCg     >  1:1156129/1‑93 (MQ=255)

                                                                                    tACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCg     <  1:4241748/93‑1 (MQ=255)

                                                                                    tACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCg     >  1:9536/1‑93 (MQ=255)

                                                                                     aCAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGa    <  1:5250784/93‑1 (MQ=255)

                                                                                     aCAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGa    <  2:3504127/93‑1 (MQ=255)

                                                                                      cAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAg   >  2:3739197/1‑93 (MQ=255)

CCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGC  >  NC_000913/667883‑668062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGT  >  NC_000913/667880‑668065

AAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGT                                                                                              <  SRR4302533.5728536/94‑1 (MQ=60)

 AACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTG                                                                                             >  SRR4302533.1047681/1‑94 (MQ=60)

 AACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTG                                                                                             <  SRR4302533.3623421/94‑1 (MQ=60)

 AACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTG                                                                                             <  SRR4302533.2912460/94‑1 (MQ=60)

  ACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGA                                                                                            >  SRR4302533.1966025/1‑94 (MQ=60)

   CCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGAC                                                                                           <  SRR4302533.5825033/94‑1 (MQ=60)

   CCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGAC                                                                                           <  SRR4302533.4956818/94‑1 (MQ=60)

     GGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGG                                                                                         <  SRR4302533.6325541/94‑1 (MQ=60)

      GTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGC                                                                                        >  SRR4302533.1673634/1‑94 (MQ=60)

         TGTACCCAGTACGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCT                                                                                     <  SRR4302533.6142199/94‑1 (MQ=60)

          GTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTC                                                                                    <  SRR4302533.4433386/94‑1 (MQ=60)

          GTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTC                                                                                    >  SRR4302533.32038/1‑94 (MQ=60)

           TACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCT                                                                                   <  SRR4302533.5909904/94‑1 (MQ=60)

            GCCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTT                                                                                  <  SRR4302533.5193373/94‑1 (MQ=60)

            ACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTT                                                                                  >  SRR4302533.5050438/1‑94 (MQ=60)

            ACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTT                                                                                  <  SRR4302533.2457343/94‑1 (MQ=60)

             CCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTC                                                                                 <  SRR4302533.517408/94‑1 (MQ=60)

             CCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTC                                                                                 >  SRR4302533.1066291/1‑94 (MQ=60)

               CAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCT                                                                               <  SRR4302533.5513134/94‑1 (MQ=60)

               CAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCT                                                                               <  SRR4302533.2622966/94‑1 (MQ=60)

               CAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCT                                                                               <  SRR4302533.4482179/94‑1 (MQ=60)

                AGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTG                                                                              <  SRR4302533.3261128/94‑1 (MQ=60)

                AGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTG                                                                              <  SRR4302533.1056167/94‑1 (MQ=60)

                AGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTG                                                                              >  SRR4302533.1961833/1‑94 (MQ=60)

                AGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTG                                                                              >  SRR4302533.653867/1‑94 (MQ=60)

                 GTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGC                                                                             >  SRR4302533.948535/1‑94 (MQ=60)

                 GTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGC                                                                             >  SRR4302533.5751357/1‑94 (MQ=60)

                  TCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCA                                                                            <  SRR4302533.1516631/94‑1 (MQ=60)

                  TCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCA                                                                            >  SRR4302533.2588175/1‑94 (MQ=60)

                   CCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCAT                                                                           >  SRR4302533.3439232/1‑94 (MQ=60)

                   CCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCAT                                                                           >  SRR4302533.147479/1‑94 (MQ=60)

                   CCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCAT                                                                           >  SRR4302533.4864914/1‑94 (MQ=60)

                     GGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGC                                                                         >  SRR4302533.6197467/1‑94 (MQ=60)

                        ATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACAT                                                                      >  SRR4302533.5613035/1‑94 (MQ=60)

                        ATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACAT                                                                      <  SRR4302533.3639809/94‑1 (MQ=60)

                        ATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACAT                                                                      >  SRR4302533.4187015/1‑94 (MQ=60)

                         TTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATG                                                                     <  SRR4302533.5022493/94‑1 (MQ=60)

                           TTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGA                                                                   >  SRR4302533.5920686/1‑94 (MQ=60)

                            TCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGAC                                                                  <  SRR4302533.2340138/94‑1 (MQ=60)

                            TCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGAC                                                                  <  SRR4302533.460353/94‑1 (MQ=60)

                             CGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTTCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACA                                                                 <  SRR4302533.179916/94‑1 (MQ=60)

                             CGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACA                                                                 <  SRR4302533.2268283/94‑1 (MQ=60)

                             CGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACA                                                                 <  SRR4302533.4707163/94‑1 (MQ=60)

                              GTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAA                                                                >  SRR4302533.709796/1‑94 (MQ=60)

                               TTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAAT                                                               <  SRR4302533.4343271/94‑1 (MQ=60)

                               TTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAAT                                                               >  SRR4302533.285126/1‑94 (MQ=60)

                               TTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAAT                                                               <  SRR4302533.2897551/94‑1 (MQ=60)

                                TCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATC                                                              >  SRR4302533.260365/1‑94 (MQ=60)

                                TCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATC                                                              >  SRR4302533.6099944/1‑94 (MQ=60)

                                TCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATC                                                              <  SRR4302533.2433296/94‑1 (MQ=60)

                                 CCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCA                                                             >  SRR4302533.5479633/1‑94 (MQ=60)

                                   CACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACA                                                           <  SRR4302533.2798260/94‑1 (MQ=60)

                                    ACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACAT                                                          >  SRR4302533.2208971/1‑94 (MQ=60)

                                     CGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATC                                                         <  SRR4302533.1148717/94‑1 (MQ=60)

                                     CGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATC                                                         <  SRR4302533.2936899/94‑1 (MQ=60)

                                     CGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATC                                                         <  SRR4302533.6214111/94‑1 (MQ=60)

                                      GGCGACAAGTTGCAGCTTCACGCATTAGCGCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCG                                                        <  SRR4302533.1210678/94‑1 (MQ=60)

                                        CGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCC                                                      >  SRR4302533.1418641/1‑94 (MQ=60)

                                        CGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCC                                                      <  SRR4302533.2187844/94‑1 (MQ=60)

                                        CGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGATCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCC                                                      >  SRR4302533.4464238/1‑94 (MQ=60)

                                         GACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCC                                                     >  SRR4302533.3350212/1‑94 (MQ=60)

                                          ACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCA                                                    <  SRR4302533.4923894/94‑1 (MQ=60)

                                            AAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAA                                                  >  SRR4302533.2537225/1‑94 (MQ=60)

                                             AGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAAT                                                 <  SRR4302533.1949711/94‑1 (MQ=60)

                                             AGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAAT                                                 >  SRR4302533.1544073/1‑94 (MQ=60)

                                                TGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCA                                              >  SRR4302533.5923918/1‑94 (MQ=60)

                                                  CAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACG                                            <  SRR4302533.6040494/94‑1 (MQ=60)

                                                  CAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACG                                            <  SRR4302533.2027631/94‑1 (MQ=60)

                                                    GCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGAC                                          <  SRR4302533.6185849/94‑1 (MQ=60)

                                                     CTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACA                                         >  SRR4302533.5919730/1‑94 (MQ=60)

                                                     CTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACAGGGACAATCACATCGCCCAAATCCACGACA                                         >  SRR4302533.2016737/1‑94 (MQ=60)

                                                      TTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAA                                        <  SRR4302533.5831343/94‑1 (MQ=60)

                                                       TCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAAT                                       <  SRR4302533.3474612/94‑1 (MQ=60)

                                                       TCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAAT                                       >  SRR4302533.2492050/1‑94 (MQ=60)

                                                       TCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAAT                                       >  SRR4302533.4600609/1‑94 (MQ=60)

                                                       TCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAAT                                       <  SRR4302533.4228772/94‑1 (MQ=60)

                                                        CACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATC                                      >  SRR4302533.5743914/1‑94 (MQ=60)

                                                        CACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATC                                      <  SRR4302533.991961/94‑1 (MQ=60)

                                                        CACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATC                                      >  SRR4302533.42239/1‑94 (MQ=60)

                                                         ACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCC                                     <  SRR4302533.5873739/94‑1 (MQ=60)

                                                         ACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCC                                     >  SRR4302533.5264155/1‑94 (MQ=60)

                                                          CGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCA                                    >  SRR4302533.748474/1‑94 (MQ=60)

                                                          CGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCA                                    <  SRR4302533.1737334/94‑1 (MQ=60)

                                                           GCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAG                                   <  SRR4302533.5879754/94‑1 (MQ=60)

                                                           GCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAG                                   >  SRR4302533.882979/1‑94 (MQ=60)

                                                           GCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAG                                   >  SRR4302533.1818757/1‑94 (MQ=60)

                                                           GCATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAG                                   <  SRR4302533.3362208/94‑1 (MQ=60)

                                                            CATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGT                                  <  SRR4302533.2643009/94‑1 (MQ=60)

                                                            CATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGT                                  <  SRR4302533.4813156/94‑1 (MQ=60)

                                                            CATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGT                                  >  SRR4302533.1556375/1‑94 (MQ=60)

                                                             ATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCGTGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTC                                 >  SRR4302533.3633198/1‑94 (MQ=60)

                                                             ATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTC                                 >  SRR4302533.1868801/1‑94 (MQ=60)

                                                             ATTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTC                                 >  SRR4302533.5585802/1‑94 (MQ=60)

                                                              TTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCG                                <  SRR4302533.1489152/94‑1 (MQ=60)

                                                              TTAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCG                                <  SRR4302533.1221453/94‑1 (MQ=60)

                                                               TAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGG                               <  SRR4302533.2073332/94‑1 (MQ=60)

                                                               TAACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGG                               >  SRR4302533.1086435/1‑94 (MQ=60)

                                                                AACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGC                              >  SRR4302533.4920182/1‑94 (MQ=60)

                                                                AACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGC                              >  SRR4302533.2511752/1‑94 (MQ=60)

                                                                 ACTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCG                             >  SRR4302533.84136/1‑94 (MQ=60)

                                                                  CTCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGC                            <  SRR4302533.5421861/94‑1 (MQ=60)

                                                                   TCCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCT                           >  SRR4302533.708487/1‑94 (MQ=60)

                                                                    CCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTG                          >  SRR4302533.314726/1‑94 (MQ=60)

                                                                    CCAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTG                          >  SRR4302533.6077391/1‑94 (MQ=60)

                                                                     CAGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGT                         >  SRR4302533.2233700/1‑94 (MQ=60)

                                                                      AGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTT                        >  SRR4302533.1416132/1‑94 (MQ=60)

                                                                      AGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTT                        <  SRR4302533.644120/94‑1 (MQ=60)

                                                                      AGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTT                        >  SRR4302533.3886436/1‑94 (MQ=60)

                                                                      AGAGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTT                        >  SRR4302533.804442/1‑94 (MQ=60)

                                                                        AGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTT                      >  SRR4302533.2575455/1‑94 (MQ=60)

                                                                        AGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTT                      <  SRR4302533.2708043/94‑1 (MQ=60)

                                                                        AGTTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTT                      >  SRR4302533.4911876/1‑94 (MQ=60)

                                                                        AGTTTTTCCAGTTCATACAGGGGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTT                      >  SRR4302533.3151100/1‑94 (MQ=60)

                                                                          TTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCA                    <  SRR4302533.2771664/94‑1 (MQ=60)

                                                                          TTTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCA                    <  SRR4302533.2526751/94‑1 (MQ=60)

                                                                           TTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCAC                   <  SRR4302533.6011840/94‑1 (MQ=60)

                                                                           TTTTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCAC                   <  SRR4302533.4542967/94‑1 (MQ=60)

                                                                             TTCCAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCT                 >  SRR4302533.4406452/1‑94 (MQ=60)

                                                                              TCCAGTTCATACAGGTGACGGCTCTCTTCCTGGATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTT                >  SRR4302533.3119181/1‑94 (MQ=60)

                                                                                CAGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCT              <  SRR4302533.830894/94‑1 (MQ=60)

                                                                                 AGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTA             <  SRR4302533.257851/94‑1 (MQ=60)

                                                                                 AGTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTA             <  SRR4302533.2828230/94‑1 (MQ=60)

                                                                                  GTTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTAC            >  SRR4302533.2120020/1‑94 (MQ=60)

                                                                                   TTCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACG           >  SRR4302533.3574837/1‑94 (MQ=60)

                                                                                    TCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGC          >  SRR4302533.4379067/1‑94 (MQ=60)

                                                                                    TCATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGC          >  SRR4302533.3352536/1‑94 (MQ=60)

                                                                                     CATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCC         >  SRR4302533.1358246/1‑94 (MQ=60)

                                                                                      ATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCG        >  SRR4302533.1163061/1‑94 (MQ=60)

                                                                                      ATACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCG        >  SRR4302533.9617/1‑94 (MQ=60)

                                                                                       TACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGA       <  SRR4302533.4267363/94‑1 (MQ=60)

                                                                                        ACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAG      <  SRR4302533.5282337/94‑1 (MQ=60)

                                                                                        ACAGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAG      <  SRR4302533.1793132/94‑1 (MQ=60)

                                                                                          AGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCG    >  SRR4302533.405874/1‑94 (MQ=60)

                                                                                          AGGTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCG    <  SRR4302533.4239769/94‑1 (MQ=60)

                                                                                            GTGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGT  <  SRR4302533.3159083/94‑1 (MQ=60)

AAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGT  >  NC_000913/667880‑668065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`