Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	671,943	T→C	A688A (GCA→GCG)	leuS ←	leucine tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	671,943	0	T	C	100.0%	762.3 / NA	235	A688A (GCA→GCG)	leuS	leucine tRNA synthetase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (120/115); total (120/115)

Only 100 of 246 total aligned reads displayed.

ACGGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTG  >  NC_000913/671853‑672032

acgGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAgcgc                                                                                         <  2:5283038/93‑1 (MQ=255)

  ggCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCg                                                                                       >  2:2969467/1‑93 (MQ=255)

   gCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGcgccgc                                                                                      <  2:4108398/93‑1 (MQ=255)

       atatCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGCTTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACa                                                                                  >  2:3905340/1‑93 (MQ=255)

        tatCTTCGGTCACTTTAGCGATCTTTTTATGCACATCGCGCCGCAGCGTTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACAt                                                                                 <  2:1374276/93‑1 (MQ=255)

        tatCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACAt                                                                                 <  1:1206559/93‑1 (MQ=255)

           catcGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCAc                                                                              <  2:6261262/93‑1 (MQ=255)

           catcGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCAc                                                                              <  1:3002947/93‑1 (MQ=255)

           catcGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCAc                                                                              >  2:1872460/1‑93 (MQ=255)

              cGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCtt                                                                           <  1:5216993/93‑1 (MQ=255)

               ggTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCttt                                                                          <  1:1374997/93‑1 (MQ=255)

               ggTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCttt                                                                          >  2:1014364/1‑93 (MQ=255)

                gTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCtttt                                                                         >  1:4923375/1‑93 (MQ=255)

                gTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCtttt                                                                         <  2:3147649/93‑1 (MQ=255)

                gTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCtttt                                                                         <  2:1728016/93‑1 (MQ=255)

                 tCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTg                                                                        <  2:639096/93‑1 (MQ=255)

                  cACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTgt                                                                       >  1:4794405/1‑92 (MQ=255)

                  cACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGc                                                                       >  2:5674863/1‑93 (MQ=255)

                    cTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCtg                                                                     <  1:1120115/93‑1 (MQ=255)

                     tttAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCtgt                                                                    >  1:6072707/1‑93 (MQ=255)

                      ttAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCtgtg                                                                   >  1:3363797/1‑93 (MQ=255)

                      ttAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCtgtg                                                                   >  1:783853/1‑93 (MQ=255)

                         gCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGc                                                                >  2:91907/1‑93 (MQ=255)

                         gCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGc                                                                <  1:4676853/93‑1 (MQ=255)

                           gATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTc                                                              <  2:4389942/93‑1 (MQ=255)

                            aTCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCg                                                             >  2:5085411/1‑93 (MQ=255)

                             tCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGt                                                            <  1:4145897/93‑1 (MQ=255)

                             tCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGt                                                            >  2:4229261/1‑93 (MQ=255)

                             tCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGt                                                            >  2:3539084/1‑93 (MQ=255)

                              cGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTa                                                           <  1:3147355/93‑1 (MQ=255)

                              cGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTa                                                           <  2:1083448/93‑1 (MQ=255)

                               gTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTaa                                                          >  1:1343230/1‑93 (MQ=255)

                               gTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTaa                                                          >  1:5375101/1‑93 (MQ=255)

                                ttttATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTaaa                                                         >  2:1588314/1‑93 (MQ=255)

                                 tttATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAAc                                                        >  2:3834352/1‑93 (MQ=255)

                                  ttATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAAcc                                                       >  1:4253299/1‑93 (MQ=255)

                                   tATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCa                                                      <  1:3227659/93‑1 (MQ=255)

                                   tATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCa                                                      <  2:5754671/93‑1 (MQ=255)

                                   tATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCa                                                      >  1:1948696/1‑93 (MQ=255)

                                   tATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCa                                                      >  1:5799534/1‑93 (MQ=255)

                                    aTGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAg                                                     >  1:611627/1‑93 (MQ=255)

                                     tGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGt                                                    <  2:4075967/93‑1 (MQ=255)

                                     tGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGATGTGTGCTCGTAAACCAGt                                                    <  2:6143938/93‑1 (MQ=255)

                                        acaTCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTc                                                 >  1:2528120/1‑93 (MQ=255)

                                         caTCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTcc                                                <  1:2844540/93‑1 (MQ=255)

                                            cgcgACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGa                                             <  2:1671919/93‑1 (MQ=255)

                                              cgACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGaca                                           <  1:6053041/93‑1 (MQ=255)

                                              cgACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGaca                                           <  2:1240466/93‑1 (MQ=255)

                                               gACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGacac                                          >  1:2742991/1‑93 (MQ=255)

                                               gACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGacac                                          >  1:2492706/1‑93 (MQ=255)

                                               gACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGacac                                          >  2:4672078/1‑93 (MQ=255)

                                                  gCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGtt                                       >  1:5655263/1‑93 (MQ=255)

                                                   cAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGttt                                      >  1:4500205/1‑93 (MQ=255)

                                                   cAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGttt                                      >  2:754853/1‑93 (MQ=255)

                                                    aGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTc                                     <  1:2338072/93‑1 (MQ=255)

                                                    aGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTc                                     >  2:2530498/1‑93 (MQ=255)

                                                    aGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTc                                     >  2:5796532/1‑93 (MQ=255)

                                                    aGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTc                                     <  2:3251924/93‑1 (MQ=255)

                                                    aGCGCTTTCTGATTGTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTc                                     <  1:2613902/93‑1 (MQ=255)

                                                      cgcTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAg                                   <  2:2289097/93‑1 (MQ=255)

                                                       gcTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAgg                                  <  1:5881368/93‑1 (MQ=255)

                                                        cTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTCAACCAGTTTCCAGACACGTTTCAGGa                                 >  1:2114448/1‑93 (MQ=255)

                                                        cTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGa                                 <  1:5479991/93‑1 (MQ=255)

                                                         tttCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGaa                                >  1:2593315/1‑93 (MQ=255)

                                                         tttCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGaa                                >  2:2717603/1‑93 (MQ=255)

                                                          ttCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAg                               <  1:2202982/93‑1 (MQ=255)

                                                          ttCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAg                               >  1:5353315/1‑93 (MQ=255)

                                                          ttCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAg                               >  1:3968682/1‑93 (MQ=255)

                                                           tCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGc                              <  2:3728221/93‑1 (MQ=255)

                                                           tCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGc                              <  1:4995131/93‑1 (MQ=255)

                                                           tCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGc                              >  2:3359962/1‑93 (MQ=255)

                                                             tGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCgg                            >  1:3077552/1‑93 (MQ=255)

                                                                ttttCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTa                         >  1:5908727/1‑93 (MQ=255)

                                                                  ttCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCGCCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGc                       <  2:4836663/93‑1 (MQ=255)

                                                                     agtcagCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGcccc                    >  1:3550332/1‑93 (MQ=255)

                                                                      gtcagCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCt                   <  1:649058/93‑1 (MQ=255)

                                                                        cagCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTc                 >  1:1025967/1‑93 (MQ=255)

                                                                          gcgcATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCca               >  2:2418036/1‑93 (MQ=255)

                                                                          gcgcATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCca               >  1:173241/1‑93 (MQ=255)

                                                                             cATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCacac            >  2:2321576/1‑93 (MQ=255)

                                                                             cATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCacac            <  2:163835/93‑1 (MQ=255)

                                                                             cATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCacac            <  2:1609810/93‑1 (MQ=255)

                                                                             cATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCacac            <  1:5445066/93‑1 (MQ=255)

                                                                             cATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCacac            >  1:4078599/1‑93 (MQ=255)

                                                                              aTCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACAcc           <  2:81949/93‑1 (MQ=255)

                                                                              aTCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACAcc           <  2:3509005/93‑1 (MQ=255)

                                                                              aTCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACAcc           >  2:2187044/1‑93 (MQ=255)

                                                                                cAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCgg         >  1:5031775/1‑93 (MQ=255)

                                                                                 aaCGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACCCGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGa        <  2:4052636/93‑1 (MQ=255)

                                                                                 aaCGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGa        >  2:765471/1‑93 (MQ=255)

                                                                                 aaCGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGa        >  1:758805/1‑93 (MQ=255)

                                                                                 aaCGTTCAGCGCCGCAACATCACCTTTGGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGa        <  1:4450109/93‑1 (MQ=255)

                                                                                  aCGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGAt       >  1:4341593/1‑93 (MQ=255)

                                                                                   cGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGAtt      >  2:5597927/1‑93 (MQ=255)

                                                                                    gTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTc     <  2:4726478/93‑1 (MQ=255)

                                                                                     ttCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTcc    <  1:6011488/93‑1 (MQ=255)

                                                                                     ttCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTcc    <  2:6131890/93‑1 (MQ=255)

                                                                                     ttCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTcc    <  2:5219854/93‑1 (MQ=255)

                                                                                     ttCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTcc    <  2:1417666/93‑1 (MQ=255)

                                                                                       cAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTg  >  2:6024411/1‑93 (MQ=255)

ACGGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTG  >  NC_000913/671853‑672032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGACGGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCAT  >  NC_000913/671851‑672036

CGACGGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCG                                                                                              >  SRR4302533.4156638/1‑94 (MQ=60)

CGACGGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCG                                                                                              >  SRR4302533.2535176/1‑94 (MQ=60)

CGACGGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCG                                                                                              <  SRR4302533.1009545/94‑1 (MQ=60)

CGACGGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCG                                                                                              >  SRR4302533.2957747/1‑94 (MQ=60)

CGACGGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCG                                                                                              <  SRR4302533.6232582/94‑1 (MQ=60)

 GACGGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGTTTTTCAGTCAGCGCATCAACGTTCAGCGC                                                                                             >  SRR4302533.3273178/1‑94 (MQ=60)

    GGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGC                                                                                          >  SRR4302533.5759553/1‑94 (MQ=60)

    GGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGC                                                                                          >  SRR4302533.5703211/1‑94 (MQ=60)

       CGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAAC                                                                                       >  SRR4302533.657027/1‑94 (MQ=60)

        GATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACA                                                                                      <  SRR4302533.2665408/94‑1 (MQ=60)

        GATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACA                                                                                      >  SRR4302533.6100942/1‑94 (MQ=60)

         ATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACAT                                                                                     >  SRR4302533.5472552/1‑94 (MQ=60)

         ATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACAT                                                                                     <  SRR4302533.6084795/94‑1 (MQ=60)

         ATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACAT                                                                                     >  SRR4302533.5483163/1‑94 (MQ=60)

          TATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATC                                                                                    <  SRR4302533.1213806/94‑1 (MQ=60)

             CATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACC                                                                                 <  SRR4302533.3021311/94‑1 (MQ=60)

             CATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACC                                                                                 >  SRR4302533.4104177/1‑94 (MQ=60)

             CATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACC                                                                                 >  SRR4302533.5432058/1‑94 (MQ=60)

             CATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACC                                                                                 >  SRR4302533.4104909/1‑94 (MQ=60)

             CATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACC                                                                                 >  SRR4302533.137746/1‑94 (MQ=60)

              ATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCACCGTTCAGCGCCGCAACATCACCT                                                                                >  SRR4302533.4244918/1‑94 (MQ=60)

               TCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTT                                                                               >  SRR4302533.3331711/1‑94 (MQ=60)

               TCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTT                                                                               >  SRR4302533.1673616/1‑94 (MQ=60)

               TCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTT                                                                               <  SRR4302533.254037/94‑1 (MQ=60)

                CGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTT                                                                              <  SRR4302533.247984/94‑1 (MQ=60)

                CGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTT                                                                              <  SRR4302533.5248401/94‑1 (MQ=60)

                 GGTCACTTTAGCGATTGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTT                                                                             >  SRR4302533.2767931/1‑94 (MQ=60)

                 GGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTT                                                                             >  SRR4302533.4953203/1‑94 (MQ=60)

                 GGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTT                                                                             >  SRR4302533.5401957/1‑94 (MQ=60)

                 GGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTT                                                                             <  SRR4302533.1383270/94‑1 (MQ=60)

                  GTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTG                                                                            <  SRR4302533.783533/94‑1 (MQ=60)

                  GTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTG                                                                            <  SRR4302533.4503659/94‑1 (MQ=60)

                   TCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGT                                                                           >  SRR4302533.4823449/1‑94 (MQ=60)

                    CACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCT                                                                          <  SRR4302533.3119801/94‑1 (MQ=60)

                     ACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTG                                                                         >  SRR4302533.4586112/1‑94 (MQ=60)

                      CTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGT                                                                        <  SRR4302533.1126759/94‑1 (MQ=60)

                      CTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGT                                                                        >  SRR4302533.6109444/1‑94 (MQ=60)

                      CTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGT                                                                        <  SRR4302533.293010/94‑1 (MQ=60)

                       TTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTG                                                                       >  SRR4302533.788464/1‑94 (MQ=60)

                       TTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTG                                                                       >  SRR4302533.3384580/1‑94 (MQ=60)

                        TTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGT                                                                      >  SRR4302533.5638552/1‑94 (MQ=60)

                        TTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGT                                                                      <  SRR4302533.490122/94‑1 (MQ=60)

                           GCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCT                                                                   <  SRR4302533.4705197/94‑1 (MQ=60)

                            CGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTC                                                                  <  SRR4302533.266796/94‑1 (MQ=60)

                             GATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCG                                                                 >  SRR4302533.452885/1‑94 (MQ=60)

                              ATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTccgttttgttcga                                                                >  SRR4302533.6304586/1‑81 (MQ=60)

                              ATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGT                                                                <  SRR4302533.1714382/94‑1 (MQ=60)

                               TCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTA                                                               <  SRR4302533.4171021/94‑1 (MQ=60)

                               TCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTA                                                               >  SRR4302533.1090320/1‑94 (MQ=60)

                                CGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAA                                                              <  SRR4302533.3166707/94‑1 (MQ=60)

                                CGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAA                                                              >  SRR4302533.1351316/1‑94 (MQ=60)

                                CGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAA                                                              >  SRR4302533.5935834/1‑94 (MQ=60)

                                CGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAA                                                              >  SRR4302533.5407403/1‑94 (MQ=60)

                                 GTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAA                                                             >  SRR4302533.2154930/1‑94 (MQ=60)

                                   TTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACC                                                           >  SRR4302533.2564174/1‑94 (MQ=60)

                                   TTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACC                                                           >  SRR4302533.4278953/1‑94 (MQ=60)

                                    TTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCA                                                          >  SRR4302533.5834572/1‑94 (MQ=60)

                                    TTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCA                                                          >  SRR4302533.1960577/1‑94 (MQ=60)

                                    TTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCA                                                          >  SRR4302533.4031985/1‑94 (MQ=60)

                                     TATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAG                                                         <  SRR4302533.3247557/94‑1 (MQ=60)

                                     TATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAG                                                         >  SRR4302533.615222/1‑94 (MQ=60)

                                     TATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAG                                                         <  SRR4302533.2321659/94‑1 (MQ=60)

                                        GCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTT                                                      <  SRR4302533.5016543/94‑1 (MQ=60)

                                         CACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTC                                                     >  SRR4302533.2543684/1‑94 (MQ=60)

                                           CATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCA                                                   <  SRR4302533.2862044/94‑1 (MQ=60)

                                           CATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCA                                                   >  SRR4302533.479792/1‑94 (MQ=60)

                                            ATCGCGTCGCATCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCGCCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAG                                                  <  SRR4302533.6025780/94‑1 (MQ=60)

                                               GCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACA                                               <  SRR4302533.3863370/94‑1 (MQ=60)

                                               GCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACA                                               <  SRR4302533.1204061/94‑1 (MQ=60)

                                                CGACGCAGCGCTTTGTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACAC                                              <  SRR4302533.1290980/94‑1 (MQ=60)

                                                CGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACAC                                              <  SRR4302533.6089701/94‑1 (MQ=60)

                                                CGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACAC                                              >  SRR4302533.2759809/1‑94 (MQ=60)

                                                CGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACAC                                              >  SRR4302533.2508060/1‑94 (MQ=60)

                                                 GACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACG                                             <  SRR4302533.2921738/94‑1 (MQ=60)

                                                   CGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTT                                           >  SRR4302533.5689439/1‑94 (MQ=60)

                                                   CGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTT                                           <  SRR4302533.4316096/94‑1 (MQ=60)

                                                   CGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTT                                           >  SRR4302533.2044000/1‑94 (MQ=60)

                                                    GCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTT                                          <  SRR4302533.4448943/94‑1 (MQ=60)

                                                    GCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTT                                          <  SRR4302533.5920257/94‑1 (MQ=60)

                                                    GCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTT                                          >  SRR4302533.4527461/1‑94 (MQ=60)

                                                      AGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCA                                        <  SRR4302533.2352545/94‑1 (MQ=60)

                                                      AGCGCTTTCTGATTTGCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCA                                        <  SRR4302533.624660/94‑1 (MQ=60)

                                                      AGCGCTTTCTGATTGTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCA                                        <  SRR4302533.2629946/94‑1 (MQ=60)

                                                       GCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAG                                       <  SRR4302533.6129850/94‑1 (MQ=60)

                                                         GCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTCAACCAGTTTCCAGACACGTTTCAGGA                                     >  SRR4302533.2127488/1‑94 (MQ=60)

                                                         GCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGA                                     <  SRR4302533.5916911/94‑1 (MQ=60)

                                                         GCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGA                                     <  SRR4302533.4396269/94‑1 (MQ=60)

                                                          CTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAA                                    >  SRR4302533.3032697/1‑94 (MQ=60)

                                                          CTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAA                                    <  SRR4302533.5513029/94‑1 (MQ=60)

                                                          CTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAA                                    >  SRR4302533.2609250/1‑94 (MQ=60)

                                                           TTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAG                                   >  SRR4302533.3992855/1‑94 (MQ=60)

                                                           TTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAG                                   >  SRR4302533.5385444/1‑94 (MQ=60)

                                                           TTTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAG                                   >  SRR4302533.3157090/1‑94 (MQ=60)

                                                            TTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGC                                  <  SRR4302533.5056969/94‑1 (MQ=60)

                                                            TTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGC                                  <  SRR4302533.1880708/94‑1 (MQ=60)

                                                            TTCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGC                                  <  SRR4302533.2216561/94‑1 (MQ=60)

                                                             TCTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCG                                 <  SRR4302533.5025412/94‑1 (MQ=60)

                                                              CTGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGG                                >  SRR4302533.3096437/1‑94 (MQ=60)

                                                               TGATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGT                               <  SRR4302533.2254407/94‑1 (MQ=60)

                                                                 ATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTA                             >  SRR4302533.5944421/1‑94 (MQ=60)

                                                                 ATTTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTA                             <  SRR4302533.3237165/94‑1 (MQ=60)

                                                                   TTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGC                           <  SRR4302533.5378806/94‑1 (MQ=60)

                                                                   TTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGC                           <  SRR4302533.5328332/94‑1 (MQ=60)

                                                                   TTTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGC                           <  SRR4302533.5036842/94‑1 (MQ=60)

                                                                    TTCAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCC                          >  SRR4302533.2623537/1‑94 (MQ=60)

                                                                      CAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCC                        <  SRR4302533.121946/94‑1 (MQ=60)

                                                                      CAGTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCC                        >  SRR4302533.3572205/1‑94 (MQ=60)

                                                                        GTCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTT                      <  SRR4302533.652886/94‑1 (MQ=60)

                                                                         TCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTC                     >  SRR4302533.1031996/1‑94 (MQ=60)

                                                                         TCAGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTC                     >  SRR4302533.3661252/1‑94 (MQ=60)

                                                                           AGCGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCA                   >  SRR4302533.174333/1‑94 (MQ=60)

                                                                             CGCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACA                 >  SRR4302533.2090480/1‑94 (MQ=60)

                                                                              GCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACAC                >  SRR4302533.3347114/1‑94 (MQ=60)

                                                                              GCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACAC                <  SRR4302533.708788/94‑1 (MQ=60)

                                                                              GCATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACAC                >  SRR4302533.4103428/1‑94 (MQ=60)

                                                                               CATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACC               <  SRR4302533.5477873/94‑1 (MQ=60)

                                                                                ATGAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCG              >  SRR4302533.6123708/1‑94 (MQ=60)

                                                                                ATCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCG              >  SRR4302533.5912302/1‑94 (MQ=60)

                                                                                 TCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGG             <  SRR4302533.3465290/94‑1 (MQ=60)

                                                                                 TCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGG             >  SRR4302533.5062240/1‑94 (MQ=60)

                                                                                 TCAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGG             <  SRR4302533.4883288/94‑1 (MQ=60)

                                                                                  CAACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGA            >  SRR4302533.763272/1‑94 (MQ=60)

                                                                                   AACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGAT           >  SRR4302533.4367757/1‑94 (MQ=60)

                                                                                   AACGTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGAT           <  SRR4302533.2524146/94‑1 (MQ=60)

                                                                                   AACGTTCAGCGCCGCAACATCACCTTTGGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGAT           <  SRR4302533.4477018/94‑1 (MQ=60)

                                                                                      GTTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCC        >  SRR4302533.5235256/1‑94 (MQ=60)

                                                                                       TTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCT       >  SRR4302533.6067746/1‑94 (MQ=60)

                                                                                       TTCAGCGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCT       <  SRR4302533.6047908/94‑1 (MQ=60)

                                                                                            CGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCAT  <  SRR4302533.545542/94‑1 (MQ=60)

CGACGGCCGATATCATCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCAT  >  NC_000913/671851‑672036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`