Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	741,626	T→C	S52G (AGC→GGC)	ybgH ←	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	741,626	0	T	C	99.5%	692.5 / ‑4.8	216	S52G (AGC→GGC)	ybgH	putative transport protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (120/95); total (120/96)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

Only 100 of 219 total aligned reads displayed.

CCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAAT  >  NC_000913/741536‑741715

ccAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCaa                                                                                         >  2:65200/1‑93 (MQ=255)

 cAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCaaa                                                                                        <  1:4495841/93‑1 (MQ=255)

 cAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCaaa                                                                                        >  1:6156275/1‑93 (MQ=255)

  aGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAAt                                                                                       <  2:4781335/93‑1 (MQ=255)

  aGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAAt                                                                                       >  2:2942227/1‑93 (MQ=255)

   gCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATa                                                                                      <  1:5729058/93‑1 (MQ=255)

     aTCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAAc                                                                                    <  1:6044619/93‑1 (MQ=255)

      tCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACt                                                                                   >  2:602593/1‑93 (MQ=255)

       cACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTc                                                                                  <  1:5879106/93‑1 (MQ=255)

        aCCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCg                                                                                 <  1:4656439/93‑1 (MQ=255)

        aCCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCg                                                                                 >  2:2148398/1‑93 (MQ=255)

         ccGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGt                                                                                >  1:2724568/1‑93 (MQ=255)

          cGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTa                                                                               <  1:89083/93‑1 (MQ=255)

            ccATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAgg                                                                             >  1:3215122/1‑93 (MQ=255)

             cATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGc                                                                            <  2:876543/93‑1 (MQ=255)

             cATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGc                                                                            >  2:1540774/1‑93 (MQ=255)

              aTGCGATTGCCGAGAACTTTATCCGCCAAAAGGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCg                                                                           <  2:3465079/93‑1 (MQ=255)

              aTGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCg                                                                           <  2:1742130/93‑1 (MQ=255)

              aTGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCg                                                                           <  1:4813158/93‑1 (MQ=255)

              aTGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCg                                                                           <  1:3571763/93‑1 (MQ=255)

               tGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGt                                                                          <  1:2263653/93‑1 (MQ=255)

                 cGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGa                                                                        <  1:5845737/93‑1 (MQ=255)

                  gATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAg                                                                       >  2:1135954/1‑93 (MQ=255)

                  gATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAg                                                                       >  2:742685/1‑93 (MQ=255)

                  gATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAg                                                                       >  1:1667548/1‑93 (MQ=255)

                    ttGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTa                                                                     >  1:4046106/1‑93 (MQ=255)

                    ttGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTa                                                                     >  2:233599/1‑93 (MQ=255)

                    ttGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTa                                                                     >  1:2223591/1‑93 (MQ=255)

                     tGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGCAt                                                                    >  1:1944812/1‑93 (MQ=255)

                      gCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATc                                                                   >  2:1402538/1‑93 (MQ=255)

                        cGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGt                                                                 >  2:619840/1‑93 (MQ=255)

                        cGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGt                                                                 >  1:1090256/1‑93 (MQ=255)

                        cGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGt                                                                 <  1:4107048/93‑1 (MQ=255)

                        cGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGt                                                                 <  1:32664/93‑1 (MQ=255)

                         gagaACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGtt                                                                <  1:4154078/93‑1 (MQ=255)

                         gagaACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGtt                                                                >  2:5579607/1‑93 (MQ=255)

                         gagaACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGtt                                                                <  1:1888451/93‑1 (MQ=255)

                          agaACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTCGGCGTGAGTATCGTTg                                                               >  1:408781/1‑93 (MQ=255)

                           gaACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGt                                                              >  1:2250942/1‑93 (MQ=255)

                           gaACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGt                                                              <  1:1754602/93‑1 (MQ=255)

                            aaCTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTa                                                             <  1:3872317/93‑1 (MQ=255)

                               tttATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTAttt                                                          >  1:159545/1‑93 (MQ=255)

                               tttATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTAttt                                                          >  1:5034031/1‑93 (MQ=255)

                               tttATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTAttt                                                          >  2:5557807/1‑93 (MQ=255)

                                ttATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTAtttt                                                         >  2:3606194/1‑93 (MQ=255)

                                ttATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTAtttt                                                         >  2:2898760/1‑93 (MQ=255)

                                 tATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTa                                                        <  2:445520/93‑1 (MQ=255)

                                 tATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTa                                                        <  2:2687826/93‑1 (MQ=255)

                                   tCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGt                                                      >  1:370385/1‑93 (MQ=255)

                                   tCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGt                                                      >  1:4686556/1‑93 (MQ=255)

                                     cGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTg                                                    <  1:2216331/93‑1 (MQ=255)

                                       ccAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGAt                                                  >  1:303800/1‑93 (MQ=255)

                                        cAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGGAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGAtt                                                 <  1:3680609/93‑1 (MQ=255)

                                          aaaaGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTgg                                               >  2:4924893/1‑93 (MQ=255)

                                           aaaGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTggg                                              >  2:5907626/1‑92 (MQ=255)

                                           aaaGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGt                                              <  2:5467758/93‑1 (MQ=255)

                                            aaGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTg                                             <  1:2453281/93‑1 (MQ=255)

                                            aaGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTg                                             >  2:1968496/1‑93 (MQ=255)

                                              gCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTgag                                           >  2:61413/1‑93 (MQ=255)

                                               ccACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTgaga                                          <  1:5896461/93‑1 (MQ=255)

                                                 aCCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATa                                        >  1:5403244/1‑93 (MQ=255)

                                                 aCCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATa                                        <  1:2024558/93‑1 (MQ=255)

                                                  ccGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATag                                       >  1:1635482/1‑93 (MQ=255)

                                                  ccGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATag                                       >  1:345534/1‑93 (MQ=255)

                                                   cGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATaga                                      <  2:908086/93‑1 (MQ=255)

                                                   cGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATaga                                      >  2:3379294/1‑93 (MQ=255)

                                                   cGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATaga                                      >  2:2056065/1‑93 (MQ=255)

                                                    gAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATagag                                     >  2:2662913/1‑93 (MQ=255)

                                                     aGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAgaga                                    >  2:5750919/1‑93 (MQ=255)

                                                      ggATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGaa                                   <  2:1496137/93‑1 (MQ=255)

                                                       gATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAAt                                  <  1:4804126/93‑1 (MQ=255)

                                                        aTTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATc                                 <  2:328114/93‑1 (MQ=255)

                                                         ttGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATca                                <  1:689500/93‑1 (MQ=255)

                                                         ttGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATca                                >  2:1317414/1‑93 (MQ=255)

                                                           ggCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGCGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcagc                              <  1:3391515/93‑1 (MQ=255)

                                                             cGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcagcag                            <  1:750213/93‑1 (MQ=255)

                                                             cGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATcagcag                            <  1:2555204/93‑1 (MQ=255)

                                                               tGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAggg                          >  1:290119/1‑93 (MQ=255)

                                                               tGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAggg                          <  2:2446368/93‑1 (MQ=255)

                                                               tGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAggg                          >  2:3630070/1‑93 (MQ=255)

                                                                   aTACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACg                      <  2:820765/93‑1 (MQ=255)

                                                                    tACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGc                     >  2:4853699/1‑93 (MQ=255)

                                                                    tACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGc                     >  1:2666229/1‑93 (MQ=255)

                                                                      cacCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCAt                   >  1:1214107/1‑93 (MQ=255)

                                                                         cAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGcc                >  1:580613/1‑93 (MQ=255)

                                                                          aGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCg               >  2:5994006/1‑93 (MQ=255)

                                                                            cGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTa             <  1:952455/93‑1 (MQ=255)

                                                                            cGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTa             >  1:5271863/1‑93 (MQ=255)

                                                                             gAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTaa            >  1:4024856/1‑93 (MQ=255)

                                                                              aaCAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTaaa           <  2:511466/93‑1 (MQ=255)

                                                                              aaCAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTaaa           <  1:2075885/93‑1 (MQ=255)

                                                                               aCAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTaaaa          <  2:3052667/93‑1 (MQ=255)

                                                                               aCAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTaaaa          <  1:1017128/93‑1 (MQ=255)

                                                                               aCAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTaaaa          >  1:2786378/1‑93 (MQ=255)

                                                                                cAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAg         <  1:3768499/93‑1 (MQ=255)

                                                                                cAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAg         >  1:5302441/1‑93 (MQ=255)

                                                                                cAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAg         <  1:2380625/93‑1 (MQ=255)

                                                                                cAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAg         >  1:5483454/1‑93 (MQ=255)

                                                                                    aGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaa     <  2:5612235/93‑1 (MQ=255)

                                                                                      gcgcCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTaaaa   <  2:3758649/93‑1 (MQ=255)

CCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAAT  >  NC_000913/741536‑741715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTC  >  NC_000913/741534‑741719

CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCA                                                                                              <  SRR4302533.1125527/94‑1 (MQ=60)

CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCA                                                                                              >  SRR4302533.3438250/1‑94 (MQ=60)

  CCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAA                                                                                            >  SRR4302533.6193311/1‑94 (MQ=60)

   CAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAAT                                                                                           <  SRR4302533.4523065/94‑1 (MQ=60)

   CAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAAT                                                                                           >  SRR4302533.2327576/1‑94 (MQ=60)

   CAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAAT                                                                                           <  SRR4302533.4083151/94‑1 (MQ=60)

    AGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATA                                                                                          >  SRR4302533.4373097/1‑94 (MQ=60)

    AGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATA                                                                                          <  SRR4302533.570585/94‑1 (MQ=60)

    AGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATA                                                                                          >  SRR4302533.182421/1‑94 (MQ=60)

     GCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAA                                                                                         >  SRR4302533.274703/1‑94 (MQ=60)

     GCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAA                                                                                         <  SRR4302533.5763653/94‑1 (MQ=60)

       ATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACT                                                                                       <  SRR4302533.6081237/94‑1 (MQ=60)

       ATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACT                                                                                       <  SRR4302533.5315507/94‑1 (MQ=60)

       ATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACT                                                                                       >  SRR4302533.5398457/1‑94 (MQ=60)

         CACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCG                                                                                     <  SRR4302533.4031328/94‑1 (MQ=60)

         CACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCG                                                                                     <  SRR4302533.5914639/94‑1 (MQ=60)

          ACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGT                                                                                    <  SRR4302533.4684657/94‑1 (MQ=60)

          ACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGT                                                                                    >  SRR4302533.2741285/1‑94 (MQ=60)

           CCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTA                                                                                   <  SRR4302533.1644907/94‑1 (MQ=60)

            CGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAG                                                                                  <  SRR4302533.89648/94‑1 (MQ=60)

             GCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGG                                                                                 >  SRR4302533.3234944/1‑94 (MQ=60)

             GCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGG                                                                                 <  SRR4302533.1948269/94‑1 (MQ=60)

              CCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGG                                                                                >  SRR4302533.2058659/1‑94 (MQ=60)

               CATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCG                                                                               >  SRR4302533.520058/1‑94 (MQ=60)

                ATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGT                                                                              <  SRR4302533.3593774/94‑1 (MQ=60)

                ATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGT                                                                              <  SRR4302533.4842328/94‑1 (MQ=60)

                 TGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTG                                                                             >  SRR4302533.5063065/1‑94 (MQ=60)

                 TGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTG                                                                             <  SRR4302533.2277660/94‑1 (MQ=60)

                   CGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAG                                                                           >  SRR4302533.2609321/1‑94 (MQ=60)

                   CGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAG                                                                           >  SRR4302533.1677622/1‑94 (MQ=60)

                   CGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAG                                                                           <  SRR4302533.2261248/94‑1 (MQ=60)

                   CGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAG                                                                           <  SRR4302533.5881078/94‑1 (MQ=60)

                   CGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAG                                                                           <  SRR4302533.1979945/94‑1 (MQ=60)

                   CGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAG                                                                           >  SRR4302533.3516766/1‑94 (MQ=60)

                     ATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTA                                                                         >  SRR4302533.4070733/1‑94 (MQ=60)

                     ATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTA                                                                         >  SRR4302533.2237325/1‑94 (MQ=60)

                      TTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGCAT                                                                        >  SRR4302533.1956672/1‑94 (MQ=60)

                        GCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCG                                                                      <  SRR4302533.4837353/94‑1 (MQ=60)

                        GCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCG                                                                      <  SRR4302533.2933972/94‑1 (MQ=60)

                         CCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGT                                                                     >  SRR4302533.1096699/1‑94 (MQ=60)

                          CGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTT                                                                    <  SRR4302533.4132020/94‑1 (MQ=60)

                          CGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTT                                                                    >  SRR4302533.4118001/1‑94 (MQ=60)

                          CGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTT                                                                    <  SRR4302533.32886/94‑1 (MQ=60)

                          CGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTT                                                                    <  SRR4302533.1370777/94‑1 (MQ=60)

                          CGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTT                                                                    >  SRR4302533.2956128/1‑94 (MQ=60)

                           GAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTCGGCGTGAGTATCGTTG                                                                   >  SRR4302533.411234/1‑94 (MQ=60)

                           GAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTG                                                                   <  SRR4302533.1899989/94‑1 (MQ=60)

                           GAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTG                                                                   <  SRR4302533.4179249/94‑1 (MQ=60)

                           GAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTG                                                                   <  SRR4302533.4574288/94‑1 (MQ=60)

                            AGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGT                                                                  >  SRR4302533.2264856/1‑94 (MQ=60)

                             GAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTA                                                                 >  SRR4302533.2723897/1‑94 (MQ=60)

                             GAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTA                                                                 <  SRR4302533.1765287/94‑1 (MQ=60)

                             GAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTA                                                                 <  SRR4302533.5910868/94‑1 (MQ=60)

                             GAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTA                                                                 >  SRR4302533.5914791/1‑94 (MQ=60)

                              AACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTAT                                                                >  SRR4302533.1672791/1‑94 (MQ=60)

                              AACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTAT                                                                <  SRR4302533.3895952/94‑1 (MQ=60)

                                CTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACTCCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTT                                                              <  SRR4302533.4061721/94‑1 (MQ=60)

                                CTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTT                                                              >  SRR4302533.5064525/1‑94 (MQ=60)

                                CTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTT                                                              >  SRR4302533.160564/1‑94 (MQ=60)

                                   TATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAG                                                           <  SRR4302533.1030847/94‑1 (MQ=60)

                                    ATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGT                                                          >  SRR4302533.4714946/1‑94 (MQ=60)

                                    ATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGT                                                          >  SRR4302533.372622/1‑94 (MQ=60)

                                       CGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGA                                                       <  SRR4302533.2230013/94‑1 (MQ=60)

                                        GCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGAT                                                      >  SRR4302533.305655/1‑94 (MQ=60)

                                        GCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGAT                                                      >  SRR4302533.2525229/1‑94 (MQ=60)

                                          CAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGGAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTG                                                    <  SRR4302533.3703227/94‑1 (MQ=60)

                                             AAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTG                                                 >  SRR4302533.2443395/1‑94 (MQ=60)

                                             AAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTG                                                 >  SRR4302533.2464/1‑94 (MQ=60)

                                              AAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGA                                                <  SRR4302533.2468421/94‑1 (MQ=60)

                                                GCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGATGAGA                                              <  SRR4302533.4444215/94‑1 (MQ=60)

                                                 CCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGAT                                             <  SRR4302533.3713377/94‑1 (MQ=60)

                                                 CCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGAT                                             <  SRR4302533.5932068/94‑1 (MQ=60)

                                                  CACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATA                                            >  SRR4302533.6012201/1‑94 (MQ=60)

                                                  CACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATA                                            <  SRR4302533.389287/94‑1 (MQ=60)

                                                  CACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATA                                            >  SRR4302533.5435711/1‑94 (MQ=60)

                                                  CACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATA                                            >  SRR4302533.1753318/1‑94 (MQ=60)

                                                   ACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAG                                           >  SRR4302533.1645387/1‑94 (MQ=60)

                                                   ACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAG                                           >  SRR4302533.347646/1‑94 (MQ=60)

                                                   ACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAG                                           <  SRR4302533.2037058/94‑1 (MQ=60)

                                                    CCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGA                                          >  SRR4302533.4157302/1‑94 (MQ=60)

                                                     CGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAG                                         >  SRR4302533.5235918/1‑94 (MQ=60)

                                                     CGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAG                                         <  SRR4302533.112144/94‑1 (MQ=60)

                                                      GAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGA                                        <  SRR4302533.5501869/94‑1 (MQ=60)

                                                       AGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAA                                       >  SRR4302533.2090556/1‑94 (MQ=60)

                                                        GGATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAAT                                      <  SRR4302533.863294/94‑1 (MQ=60)

                                                         GATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATC                                     <  SRR4302533.2127273/94‑1 (MQ=60)

                                                         GATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATC                                     <  SRR4302533.4833219/94‑1 (MQ=60)

                                                          ATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCA                                    >  SRR4302533.3960560/1‑94 (MQ=60)

                                                          ATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCA                                    <  SRR4302533.6217429/94‑1 (MQ=60)

                                                          ATTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCA                                    <  SRR4302533.4740351/94‑1 (MQ=60)

                                                           TTGGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAG                                   <  SRR4302533.693578/94‑1 (MQ=60)

                                                             GGCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGCGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCA                                 <  SRR4302533.3412459/94‑1 (MQ=60)

                                                              GCGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAG                                <  SRR4302533.3145228/94‑1 (MQ=60)

                                                               CGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGG                               <  SRR4302533.2570922/94‑1 (MQ=60)

                                                               CGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGG                               <  SRR4302533.3680787/94‑1 (MQ=60)

                                                               CGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGG                               <  SRR4302533.2270648/94‑1 (MQ=60)

                                                               CGTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGG                               <  SRR4302533.754631/94‑1 (MQ=60)

                                                                GTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGG                              >  SRR4302533.291885/1‑94 (MQ=60)

                                                                GTGACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGG                              >  SRR4302533.3451243/1‑94 (MQ=60)

                                                                  GACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCA                            <  SRR4302533.5813620/94‑1 (MQ=60)

                                                                   ACATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGAAC                           >  SRR4302533.71810/1‑94 (MQ=60)

                                                                    CATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACG                          <  SRR4302533.2809843/94‑1 (MQ=60)

                                                                     ATACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGC                         >  SRR4302533.2682587/1‑94 (MQ=60)

                                                                       ACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCAT                       >  SRR4302533.1221403/1‑94 (MQ=60)

                                                                       ACACCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCAT                       <  SRR4302533.3314507/94‑1 (MQ=60)

                                                                          CCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCC                    >  SRR4302533.584029/1‑94 (MQ=60)

                                                                          CCAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCC                    >  SRR4302533.584030/1‑94 (MQ=60)

                                                                           CAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCG                   >  SRR4302533.926741/1‑94 (MQ=60)

                                                                           CAGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCG                   <  SRR4302533.5821717/94‑1 (MQ=60)

                                                                            AGCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGT                  <  SRR4302533.3485750/94‑1 (MQ=60)

                                                                             GCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTA                 >  SRR4302533.5303478/1‑94 (MQ=60)

                                                                             GCGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTA                 >  SRR4302533.3757179/1‑94 (MQ=60)

                                                                              CGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAA                <  SRR4302533.958050/94‑1 (MQ=60)

                                                                              CGAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAA                >  SRR4302533.4049361/1‑94 (MQ=60)

                                                                               GAACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAA               >  SRR4302533.2230908/1‑94 (MQ=60)

                                                                                AACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAA              <  SRR4302533.2088662/94‑1 (MQ=60)

                                                                                AACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAA              >  SRR4302533.2803500/1‑94 (MQ=60)

                                                                                 ACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGTACGCATGCCGTAAAAG             >  SRR4302533.610185/1‑94 (MQ=60)

                                                                                 ACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAG             <  SRR4302533.1023106/94‑1 (MQ=60)

                                                                                 ACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAG             >  SRR4302533.5334209/1‑94 (MQ=60)

                                                                                 ACAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAG             >  SRR4302533.5516505/1‑94 (MQ=60)

                                                                                  CAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGC            <  SRR4302533.2395350/94‑1 (MQ=60)

                                                                                  CAGTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGC            <  SRR4302533.3791607/94‑1 (MQ=60)

                                                                                    GTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTA          >  SRR4302533.3242404/1‑94 (MQ=60)

                                                                                    GTAGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTA          >  SRR4302533.1689149/1‑94 (MQ=60)

                                                                                      AGGCGCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAA        <  SRR4302533.4711306/94‑1 (MQ=60)

                                                                                          GCCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATAT    <  SRR4302533.3935712/94‑1 (MQ=60)

                                                                                           CCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATT   <  SRR4302533.1319564/94‑1 (MQ=60)

                                                                                           CCAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATT   <  SRR4302533.6232776/94‑1 (MQ=60)

                                                                                            CAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTC  >  SRR4302533.4489220/1‑94 (MQ=60)

                                                                                            CAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTC  <  SRR4302533.3238934/94‑1 (MQ=60)

CCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTC  >  NC_000913/741534‑741719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`