Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 775,372 G→A A101T (GCC→ACC)  tolR → TolF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913775,3720GA100.0% 879.0 / NA 242A101T (GCC→ACC) tolRTolF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (108/134);  total (108/134)

Only 100 of 245 total aligned reads displayed.

ATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGC  >  NC_000913/775282‑775460
                                                                                          |                                                                                        
aTTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGAcc                                                                                        >  1:1053987/1‑93 (MQ=255)
  tGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCaa                                                                                      >  1:5291175/1‑93 (MQ=255)
   ggTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAAc                                                                                     >  2:1338366/1‑93 (MQ=255)
   ggTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAAc                                                                                     <  1:3188661/93‑1 (MQ=255)
    gTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAAcc                                                                                    <  1:33169/93‑1 (MQ=255)
     tCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAAccc                                                                                   >  2:2968050/1‑93 (MQ=255)
     tCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAAccc                                                                                   >  2:2264997/1‑93 (MQ=255)
      cAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCg                                                                                  >  1:2988592/1‑93 (MQ=255)
       aGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGa                                                                                 >  2:4182455/1‑93 (MQ=255)
       aGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGa                                                                                 >  1:5394305/1‑93 (MQ=255)
        gTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGaa                                                                                >  1:5890677/1‑93 (MQ=255)
         tACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGaaa                                                                               <  1:64453/93‑1 (MQ=255)
          acacCGTGGTGGTTGAGAAAGATAGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGCGTCCAGCCGTTTCAAGACGAACCCGaaaa                                                                              >  2:5549262/1‑93 (MQ=255)
            acCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACg                                                                            >  2:871331/1‑93 (MQ=255)
            acCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACg                                                                            >  2:4622330/1‑93 (MQ=255)
            acCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACg                                                                            >  1:6027862/1‑93 (MQ=255)
             ccGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACgg                                                                           <  1:1586655/93‑1 (MQ=255)
              cGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGt                                                                          <  2:6286387/93‑1 (MQ=255)
               gtggtgGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTc                                                                         >  1:360253/1‑93 (MQ=255)
                tggtggTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCt                                                                        <  2:5938733/93‑1 (MQ=255)
                tggtggTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCt                                                                        <  1:841595/93‑1 (MQ=255)
                tggtggTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCt                                                                        >  2:5397507/1‑93 (MQ=255)
                   tggtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTc                                                                     >  1:4750265/1‑93 (MQ=255)
                   tggtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTc                                                                     <  2:2524330/93‑1 (MQ=255)
                     gtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTg                                                                   <  1:2009467/93‑1 (MQ=255)
                     gtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTg                                                                   <  1:3656120/93‑1 (MQ=255)
                      gtGAGAAAGACCGCCTGGGGAGTTTACCCCCAGAGGAGGTGGTGGCGGAGGTGTCCAGCCGTTTCAAGACCACCCCGAAAACGGTCTTTCTGa                                                                  <  2:3954886/92‑1 (MQ=255)
                        gagaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATc                                                                >  1:3599346/1‑93 (MQ=255)
                         agaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCg                                                               >  1:2593288/1‑93 (MQ=255)
                         agaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCg                                                               <  1:1392724/93‑1 (MQ=255)
                         agaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCg                                                               <  2:1425498/93‑1 (MQ=255)
                          gaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCgg                                                              >  1:3463398/1‑93 (MQ=255)
                          gaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCgg                                                              <  2:2121830/93‑1 (MQ=255)
                               aTCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGgcg                                                         >  1:1182790/1‑93 (MQ=255)
                                tCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGgcgc                                                        <  1:1004454/93‑1 (MQ=255)
                                   ccTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCaaa                                                     >  2:3014916/1‑93 (MQ=255)
                                   ccTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCaaa                                                     >  1:5648772/1‑93 (MQ=255)
                                   ccTGGAGCGTTTACCACCAGAGCAGGTCGTGGCGGAAGTGTCCAGCCGTATCAAGACAAACCAGAAAACGGTCTTACTGATCGGTGGGGCgaa                                                     <  2:2755878/93‑1 (MQ=255)
                                    cTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCaaaa                                                    <  2:770466/93‑1 (MQ=255)
                                      ggAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGa                                                  <  1:1341481/93‑1 (MQ=255)
                                      ggAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGa                                                  <  1:4955497/93‑1 (MQ=255)
                                      ggAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGa                                                  >  1:5848779/1‑93 (MQ=255)
                                       gAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAt                                                 <  1:2262737/93‑1 (MQ=255)
                                        aGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAtg                                                <  1:175885/93‑1 (MQ=255)
                                        aGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAtg                                                <  1:142289/93‑1 (MQ=255)
                                         gCGTGTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAtgt                                               <  2:1583933/93‑1 (MQ=255)
                                           gTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGc                                             <  1:5577805/93‑1 (MQ=255)
                                           gTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGc                                             >  2:4818675/1‑93 (MQ=255)
                                            tttACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGcc                                            <  1:6245661/93‑1 (MQ=255)
                                            tttACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGcc                                            <  2:4838098/93‑1 (MQ=255)
                                             ttACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCt                                           >  1:3712983/1‑93 (MQ=255)
                                             ttACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCt                                           >  2:491919/1‑93 (MQ=255)
                                             ttACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCt                                           >  2:4340953/1‑93 (MQ=255)
                                             ttACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCt                                           <  2:203202/93‑1 (MQ=255)
                                             tcacccccAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCt                                           <  1:2480572/91‑1 (MQ=255)
                                               accaccAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTa                                         <  1:4179928/93‑1 (MQ=255)
                                               accaccAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTa                                         <  2:3315838/93‑1 (MQ=255)
                                                  accaGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGa                                      <  2:1767423/93‑1 (MQ=255)
                                                   ccaGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGAt                                     <  1:1671419/93‑1 (MQ=255)
                                                   ccaGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGAt                                     >  1:2132252/1‑93 (MQ=255)
                                                    caGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATg                                    >  1:1871843/1‑93 (MQ=255)
                                                     agagCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGa                                   >  1:4895695/1‑93 (MQ=255)
                                                       agCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGaaa                                 >  1:3174365/1‑93 (MQ=255)
                                                         cAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAaata                               <  2:2006133/93‑1 (MQ=255)
                                                          aGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAaataa                              >  1:4718025/1‑93 (MQ=255)
                                                           ggtggtGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAaataat                             <  1:994057/93‑1 (MQ=255)
                                                           ggtggtGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAaataat                             >  1:5467061/1‑93 (MQ=255)
                                                            gtggtgGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAAtt                            <  2:2200760/93‑1 (MQ=255)
                                                             tggtggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAGGTGCCTTACGATGAAATAATTa                           <  2:3669908/93‑1 (MQ=255)
                                                               gtggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTaaa                         <  2:5283367/93‑1 (MQ=255)
                                                               gtggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTaaa                         <  2:107659/93‑1 (MQ=255)
                                                               gtggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTaaa                         <  1:4353738/93‑1 (MQ=255)
                                                                tggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAg                        <  2:4939951/93‑1 (MQ=255)
                                                                 ggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGc                       <  2:4977721/93‑1 (MQ=255)
                                                                 ggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGc                       <  2:215456/93‑1 (MQ=255)
                                                                   cGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCAc                     <  2:1264174/93‑1 (MQ=255)
                                                                    ggAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACt                    >  1:5213663/1‑93 (MQ=255)
                                                                     gAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTg                   >  1:4818797/1‑93 (MQ=255)
                                                                     gAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTg                   >  2:4391460/1‑93 (MQ=255)
                                                                       aGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGaa                 <  1:5487263/93‑1 (MQ=255)
                                                                        gtgtCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAAc                <  2:2608547/93‑1 (MQ=255)
                                                                         tgtCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACt               <  1:3056351/93‑1 (MQ=255)
                                                                          gtCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACtt              >  1:5110331/1‑93 (MQ=255)
                                                                          gtCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACtt              <  2:4291706/93‑1 (MQ=255)
                                                                          gtCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACtt              <  1:5076765/93‑1 (MQ=255)
                                                                          gtCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACtt              <  1:4111002/93‑1 (MQ=255)
                                                                            ccAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGt            <  2:3533776/93‑1 (MQ=255)
                                                                              aGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTa          <  1:2181213/93‑1 (MQ=255)
                                                                                ccGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACa        <  2:3304924/93‑1 (MQ=255)
                                                                                ccGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACa        >  1:572894/1‑93 (MQ=255)
                                                                                ccGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACa        <  2:2498227/93‑1 (MQ=255)
                                                                                 cGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACAt       >  2:5658879/1‑93 (MQ=255)
                                                                                 cGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACAt       >  2:1286941/1‑93 (MQ=255)
                                                                                 cGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACAt       >  2:3087867/1‑93 (MQ=255)
                                                                                   tttCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAg     <  1:2937530/93‑1 (MQ=255)
                                                                                     tCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTg   >  2:3459702/1‑93 (MQ=255)
                                                                                     tCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTg   >  1:4086046/1‑93 (MQ=255)
                                                                                     tCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTg   >  2:1706304/1‑93 (MQ=255)
                                                                                     tCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTg   >  1:2781397/1‑93 (MQ=255)
                                                                                      cAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGc  <  1:2763619/93‑1 (MQ=255)
                                                                                          |                                                                                        
ATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGC  >  NC_000913/775282‑775460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTG  >  NC_000913/775280‑775465
                                                                                            |                                                                                             
GTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGAC                                                                                              >  SRR4302533.5093659/1‑94 (MQ=60)
 TATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACC                                                                                             >  SRR4302533.1060194/1‑94 (MQ=60)
 TATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACC                                                                                             >  SRR4302533.5507318/1‑94 (MQ=60)
   TTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAA                                                                                           >  SRR4302533.5322857/1‑94 (MQ=60)
    TGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAAC                                                                                          <  SRR4302533.3114642/94‑1 (MQ=60)
    TGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAAC                                                                                          <  SRR4302533.4119769/94‑1 (MQ=60)
     GGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACC                                                                                         <  SRR4302533.3208277/94‑1 (MQ=60)
     GGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACC                                                                                         <  SRR4302533.4777017/94‑1 (MQ=60)
      GTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCC                                                                                        <  SRR4302533.33395/94‑1 (MQ=60)
      GTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCC                                                                                        >  SRR4302533.6341381/1‑94 (MQ=60)
      GTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCC                                                                                        >  SRR4302533.593637/1‑94 (MQ=60)
       TCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCG                                                                                       >  SRR4302533.3006876/1‑94 (MQ=60)
       TCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCG                                                                                       <  SRR4302533.942268/94‑1 (MQ=60)
        CAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGA                                                                                      >  SRR4302533.2458678/1‑94 (MQ=60)
        CAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGA                                                                                      >  SRR4302533.5426718/1‑94 (MQ=60)
         AGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAA                                                                                     >  SRR4302533.5926261/1‑94 (MQ=60)
          GTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAA                                                                                    <  SRR4302533.6322878/94‑1 (MQ=60)
           TACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAA                                                                                   <  SRR4302533.64870/94‑1 (MQ=60)
            ACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAAC                                                                                  >  SRR4302533.3622956/1‑94 (MQ=60)
             CACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACG                                                                                 >  SRR4302533.6064389/1‑94 (MQ=60)
               CCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGT                                                                               <  SRR4302533.1596220/94‑1 (MQ=60)
                CGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCATCCGTTTCAAGACCAACCCGAAAACGGTC                                                                              <  SRR4302533.3606578/94‑1 (MQ=60)
                CGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTC                                                                              >  SRR4302533.362444/1‑94 (MQ=60)
                 GTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCT                                                                             >  SRR4302533.1103444/1‑94 (MQ=60)
                 GTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCT                                                                             >  SRR4302533.4245413/1‑94 (MQ=60)
                 GTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCT                                                                             <  SRR4302533.2157376/94‑1 (MQ=60)
                  TGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTT                                                                            <  SRR4302533.5637267/94‑1 (MQ=60)
                  TGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTT                                                                            <  SRR4302533.846515/94‑1 (MQ=60)
                    GTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTC                                                                          >  SRR4302533.4779066/1‑94 (MQ=60)
                     TGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCT                                                                         >  SRR4302533.3633514/1‑94 (MQ=60)
                     TGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCT                                                                         <  SRR4302533.2052894/94‑1 (MQ=60)
                     TGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCT                                                                         <  SRR4302533.5026481/94‑1 (MQ=60)
                      GGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTG                                                                        <  SRR4302533.1037316/94‑1 (MQ=60)
                      GGTGGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTG                                                                        <  SRR4302533.140617/94‑1 (MQ=60)
                       GTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGA                                                                       >  SRR4302533.3941569/1‑94 (MQ=60)
                       GTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGA                                                                       <  SRR4302533.3678593/94‑1 (MQ=60)
                       GTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGA                                                                       >  SRR4302533.2191966/1‑94 (MQ=60)
                       GTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGA                                                                       <  SRR4302533.2021844/94‑1 (MQ=60)
                         TGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATC                                                                     >  SRR4302533.3621489/1‑94 (MQ=60)
                          GAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCG                                                                    >  SRR4302533.2609223/1‑94 (MQ=60)
                           AGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGTTCGG                                                                   >  SRR4302533.5131503/1‑94 (MQ=60)
                           AGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGG                                                                   >  SRR4302533.3484778/1‑94 (MQ=60)
                           AGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGG                                                                   <  SRR4302533.1401116/94‑1 (MQ=60)
                           AGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGG                                                                   >  SRR4302533.2823764/1‑94 (MQ=60)
                           AGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGG                                                                   <  SRR4302533.1267588/94‑1 (MQ=60)
                            GAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGT                                                                  <  SRR4302533.5479191/94‑1 (MQ=60)
                            GAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGT                                                                  >  SRR4302533.3200718/1‑94 (MQ=60)
                             AAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTG                                                                 >  SRR4302533.5526006/1‑94 (MQ=60)
                             AAAGATCGCCTGGAGCGTTGACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTG                                                                 <  SRR4302533.1398932/94‑1 (MQ=60)
                              AAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGG                                                                <  SRR4302533.3043584/94‑1 (MQ=60)
                              AAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGG                                                                <  SRR4302533.5223330/94‑1 (MQ=60)
                                GATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCG                                                              <  SRR4302533.1880006/94‑1 (MQ=60)
                                GATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCG                                                              >  SRR4302533.1189894/1‑94 (MQ=60)
                                  TCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCA                                                            <  SRR4302533.5552165/94‑1 (MQ=60)
                                  TCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCA                                                            <  SRR4302533.1010329/94‑1 (MQ=60)
                                    GCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAA                                                          >  SRR4302533.5682920/1‑94 (MQ=60)
                                    GCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAA                                                          >  SRR4302533.166341/1‑94 (MQ=60)
                                     CCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGCTCGGTGGCGCAAAA                                                         >  SRR4302533.1463003/1‑94 (MQ=60)
                                     CCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAA                                                         >  SRR4302533.2997325/1‑94 (MQ=60)
                                      CTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAG                                                        >  SRR4302533.2533212/1‑94 (MQ=60)
                                      CTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAG                                                        <  SRR4302533.2922769/94‑1 (MQ=60)
                                       TGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGA                                                       >  SRR4302533.5884137/1‑94 (MQ=60)
                                       TGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGA                                                       <  SRR4302533.3319037/94‑1 (MQ=60)
                                       TGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGA                                                       <  SRR4302533.5248572/94‑1 (MQ=60)
                                       TGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGA                                                       >  SRR4302533.5338856/1‑94 (MQ=60)
                                        GGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAT                                                      <  SRR4302533.1349558/94‑1 (MQ=60)
                                        GGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAT                                                      <  SRR4302533.4985514/94‑1 (MQ=60)
                                        GGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAT                                                      >  SRR4302533.4971691/1‑94 (MQ=60)
                                        GGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAT                                                      <  SRR4302533.2686849/94‑1 (MQ=60)
                                         GAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATG                                                     <  SRR4302533.2276732/94‑1 (MQ=60)
                                         GAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATG                                                     <  SRR4302533.194099/94‑1 (MQ=60)
                                          AGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGT                                                    <  SRR4302533.143198/94‑1 (MQ=60)
                                          AGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGT                                                    <  SRR4302533.4886713/94‑1 (MQ=60)
                                          AGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGT                                                    <  SRR4302533.176991/94‑1 (MQ=60)
                                          AGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGT                                                    <  SRR4302533.5164324/94‑1 (MQ=60)
                                          AGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGT                                                    >  SRR4302533.3481797/1‑94 (MQ=60)
                                           GCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTG                                                   <  SRR4302533.366672/94‑1 (MQ=60)
                                             GTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCC                                                 <  SRR4302533.5611514/94‑1 (MQ=60)
                                              TTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCT                                                >  SRR4302533.3735783/1‑94 (MQ=60)
                                              TTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCT                                                <  SRR4302533.278156/94‑1 (MQ=60)
                                              TTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCT                                                <  SRR4302533.6283041/94‑1 (MQ=60)
                                               TCACCCCCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTT                                               <  SRR4302533.2495869/94‑1 (MQ=60)
                                                 ACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTAC                                             <  SRR4302533.4205244/94‑1 (MQ=60)
                                                 ACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTAC                                             <  SRR4302533.5922854/94‑1 (MQ=60)
                                                  CCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACG                                            <  SRR4302533.5439304/94‑1 (MQ=60)
                                                  CCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACG                                            >  SRR4302533.801243/1‑94 (MQ=60)
                                                    ACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGAT                                          >  SRR4302533.2145400/1‑94 (MQ=60)
                                                     CCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATG                                         >  SRR4302533.1883256/1‑94 (MQ=60)
                                                     CCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATG                                         <  SRR4302533.1681522/94‑1 (MQ=60)
                                                      CAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGA                                        >  SRR4302533.4925361/1‑94 (MQ=60)
                                                        GAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAA                                      <  SRR4302533.6233034/94‑1 (MQ=60)
                                                        GAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAA                                      >  SRR4302533.3193899/1‑94 (MQ=60)
                                                        GAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAA                                      <  SRR4302533.5889598/94‑1 (MQ=60)
                                                         AGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAAT                                     >  SRR4302533.3017045/1‑94 (MQ=60)
                                                          GCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATA                                    <  SRR4302533.1876673/94‑1 (MQ=60)
                                                          GCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATA                                    <  SRR4302533.5088973/94‑1 (MQ=60)
                                                           CAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAA                                   <  SRR4302533.579903/94‑1 (MQ=60)
                                                           CAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAA                                   >  SRR4302533.4746635/1‑94 (MQ=60)
                                                            AGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAAT                                  >  SRR4302533.5500012/1‑94 (MQ=60)
                                                            AGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAAT                                  <  SRR4302533.1644833/94‑1 (MQ=60)
                                                             GGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATT                                 <  SRR4302533.999867/94‑1 (MQ=60)
                                                                 GTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAG                             <  SRR4302533.4380016/94‑1 (MQ=60)
                                                                    GCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCAC                          <  SRR4302533.4886433/94‑1 (MQ=60)
                                                                     CGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACT                         >  SRR4302533.5245059/1‑94 (MQ=60)
                                                                     CGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACT                         >  SRR4302533.3285913/1‑94 (MQ=60)
                                                                      GGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTG                        >  SRR4302533.2730223/1‑94 (MQ=60)
                                                                      GGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTG                        >  SRR4302533.5727383/1‑94 (MQ=60)
                                                                      GGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTG                        >  SRR4302533.4848003/1‑94 (MQ=60)
                                                                      GGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTG                        <  SRR4302533.4846603/94‑1 (MQ=60)
                                                                         AGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAAC                     <  SRR4302533.5520342/94‑1 (MQ=60)
                                                                          GTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACT                    >  SRR4302533.5706192/1‑94 (MQ=60)
                                                                          GTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACT                    >  SRR4302533.1409330/1‑94 (MQ=60)
                                                                          GTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACT                    <  SRR4302533.119694/94‑1 (MQ=60)
                                                                          GTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACT                    <  SRR4302533.4584390/94‑1 (MQ=60)
                                                                           TGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTT                   <  SRR4302533.3075096/94‑1 (MQ=60)
                                                                           TGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTT                   >  SRR4302533.5141215/1‑94 (MQ=60)
                                                                            GTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTG                  <  SRR4302533.4135988/94‑1 (MQ=60)
                                                                            GTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTG                  <  SRR4302533.5107451/94‑1 (MQ=60)
                                                                             TCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGT                 <  SRR4302533.3593453/94‑1 (MQ=60)
                                                                              CCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTT                >  SRR4302533.1732835/1‑94 (MQ=60)
                                                                                AGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTAC              <  SRR4302533.2194661/94‑1 (MQ=60)
                                                                                 GCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACA             >  SRR4302533.576274/1‑94 (MQ=60)
                                                                                  CCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACAT            >  SRR4302533.4775945/1‑94 (MQ=60)
                                                                                  CCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACAT            <  SRR4302533.3706017/94‑1 (MQ=60)
                                                                                    GTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAG          >  SRR4302533.2394372/1‑94 (MQ=60)
                                                                                     TTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGT         <  SRR4302533.3900578/94‑1 (MQ=60)
                                                                                     TTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGT         <  SRR4302533.2955484/94‑1 (MQ=60)
                                                                                      TTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTG        >  SRR4302533.4110919/1‑94 (MQ=60)
                                                                                      TTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTG        >  SRR4302533.2204772/1‑94 (MQ=60)
                                                                                      TTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTG        <  SRR4302533.4181105/94‑1 (MQ=60)
                                                                                      TTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTG        >  SRR4302533.2798484/1‑94 (MQ=60)
                                                                                      TTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTG        >  SRR4302533.978847/1‑94 (MQ=60)
                                                                                      TTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGAACTGAACTTGTTACATAGTG        <  SRR4302533.4212692/94‑1 (MQ=60)
                                                                                        CAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCG      <  SRR4302533.2780562/94‑1 (MQ=60)
                                                                                         AAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGG     >  SRR4302533.3731596/1‑94 (MQ=60)
                                                                                         AAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGG     >  SRR4302533.2904942/1‑94 (MQ=60)
                                                                                          AGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGG    >  SRR4302533.1086294/1‑94 (MQ=60)
                                                                                            ACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTG  <  SRR4302533.461461/94‑1 (MQ=60)
                                                                                            ACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTG  <  SRR4302533.2143157/94‑1 (MQ=60)
                                                                                            |                                                                                             
GTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTG  >  NC_000913/775280‑775465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑