Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 775,372 G→A A101T (GCC→ACC)  tolR → TolF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913775,3720GA99.4% 1173.6 / ‑5.8 329A101T (GCC→ACC) tolRTolF
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (189/138);  total (189/140)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01

Only 100 of 343 total aligned reads displayed.

ATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCG  >  NC_000913/775282‑775461
                                                                                          |                                                                                         
aTTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGAcc                                                                                         <  1:4096257/93‑1 (MQ=255)
aTTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGAcc                                                                                         >  1:525017/1‑93 (MQ=255)
 ttGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCa                                                                                        >  1:4864958/1‑93 (MQ=255)
  tGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTGCCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCaa                                                                                       <  2:8032240/93‑1 (MQ=255)
  tGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCaa                                                                                       >  2:20100/1‑93 (MQ=255)
  tGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCaa                                                                                       <  1:3077264/93‑1 (MQ=255)
     tCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAAccc                                                                                    >  1:8445616/1‑93 (MQ=255)
       aGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGGTTCAAGACCAACCCGa                                                                                  >  2:154440/1‑93 (MQ=255)
       aGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTAGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGa                                                                                  >  2:5926785/1‑93 (MQ=255)
        gTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGaa                                                                                 >  2:2206611/1‑93 (MQ=255)
          acacCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGaaaa                                                                               <  1:9541051/93‑1 (MQ=255)
           cacCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAAc                                                                              >  1:4531477/1‑93 (MQ=255)
             ccGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACgg                                                                            <  2:2054495/93‑1 (MQ=255)
               gtggtgGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTc                                                                          >  2:7104655/1‑93 (MQ=255)
                tggtggTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCt                                                                         >  1:8050281/1‑93 (MQ=255)
                 ggtggtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCtt                                                                        <  1:1952566/93‑1 (MQ=255)
                  gtggtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCttt                                                                       >  1:3882025/1‑93 (MQ=255)
                   tggtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTc                                                                      >  1:5031945/1‑93 (MQ=255)
                    ggtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCt                                                                     <  1:2923983/93‑1 (MQ=255)
                    ggtTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCt                                                                     >  1:5909535/1‑93 (MQ=255)
                      ttGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGa                                                                   <  1:1335988/93‑1 (MQ=255)
                      ttGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGa                                                                   <  2:1726835/93‑1 (MQ=255)
                      ttGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGa                                                                   >  2:4478059/1‑93 (MQ=255)
                      ttGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACAAACCCGAAAACGGTCTTTCTgt                                                                   >  2:6905719/1‑92 (MQ=255)
                      gttagaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGa                                                                   <  2:7696254/90‑1 (MQ=255)
                       tGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGAt                                                                  >  1:3569467/1‑93 (MQ=255)
                       tGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGAt                                                                  >  2:4019024/1‑93 (MQ=255)
                        gagaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGagc                                                                 <  2:5646028/93‑3 (MQ=255)
                        gagaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATc                                                                 >  1:4653045/1‑93 (MQ=255)
                        gagaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATc                                                                 <  1:7568664/93‑1 (MQ=255)
                         agaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCg                                                                >  2:4807249/1‑93 (MQ=255)
                          gaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCgg                                                               <  2:4328379/93‑1 (MQ=255)
                          gaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCgg                                                               <  1:4735635/93‑1 (MQ=255)
                          gaAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCgg                                                               >  2:9247436/1‑93 (MQ=255)
                           aaaGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGt                                                              >  1:128314/1‑93 (MQ=255)
                             aGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTgg                                                            <  2:3305506/93‑1 (MQ=255)
                             aGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTgg                                                            >  2:1753550/1‑93 (MQ=255)
                                 cGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCa                                                        <  1:4392992/93‑1 (MQ=255)
                                 cGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGCCTTTCTGATCGGTGGCGCa                                                        >  2:8182362/1‑93 (MQ=255)
                                  gCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCaa                                                       >  2:8854829/1‑93 (MQ=255)
                                   ccTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCaaa                                                      >  2:937888/1‑93 (MQ=255)
                                    cTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCaaaa                                                     >  1:8274821/1‑93 (MQ=255)
                                    cTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCaaaa                                                     >  2:365964/1‑93 (MQ=255)
                                     tGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAg                                                    >  1:2497580/1‑93 (MQ=255)
                                      ggAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGa                                                   <  2:3422741/93‑1 (MQ=255)
                                       gAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAt                                                  >  1:6726748/1‑93 (MQ=255)
                                        aGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAtg                                                 <  2:9631640/93‑1 (MQ=255)
                                        aGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAtg                                                 >  1:279926/1‑93 (MQ=255)
                                          cGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAtgtg                                               >  2:3535427/1‑93 (MQ=255)
                                            tttACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGcc                                             >  2:1328610/1‑93 (MQ=255)
                                              tACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCtt                                           >  2:4902530/1‑93 (MQ=255)
                                              tACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCtt                                           <  1:6942878/93‑1 (MQ=255)
                                              tACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCtt                                           >  1:4896000/1‑93 (MQ=255)
                                                ccaccaGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTAc                                         <  1:9249604/93‑1 (MQ=255)
                                                ccaccaGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTAc                                         <  2:8627948/93‑1 (MQ=255)
                                                 caccaGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACg                                        >  2:4597106/1‑93 (MQ=255)
                                                 caccaGAGCAGGAGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACg                                        >  2:3067881/1‑93 (MQ=255)
                                                   ccaGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGAt                                      <  2:8087882/93‑1 (MQ=255)
                                                    caGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATg                                     >  2:1201738/1‑93 (MQ=255)
                                                     agagCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGa                                    >  1:2116395/1‑93 (MQ=255)
                                                     agagCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGa                                    >  2:613679/1‑93 (MQ=255)
                                                      gagCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGaa                                   >  1:3791757/1‑93 (MQ=255)
                                                      gagCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGaa                                   >  1:7115775/1‑93 (MQ=255)
                                                         cAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAaata                                <  1:9398333/93‑1 (MQ=255)
                                                           ggtggtGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAaataat                              >  2:2398879/1‑93 (MQ=255)
                                                           ggtggtGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAaataat                              >  1:3301491/1‑93 (MQ=255)
                                                            gtggtgGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAAtt                             >  1:2188954/1‑93 (MQ=255)
                                                              ggtggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTaa                           <  1:5457315/93‑1 (MQ=255)
                                                              ggtggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTaa                           <  2:5799815/93‑1 (MQ=255)
                                                               gtggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTaaa                          <  2:4255423/93‑1 (MQ=255)
                                                                tggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAg                         <  2:7777732/93‑1 (MQ=255)
                                                                 ggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGc                        <  1:9845031/93‑1 (MQ=255)
                                                                 ggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGc                        <  2:4681646/93‑1 (MQ=255)
                                                                 ggCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTGACGATGAAATAATTAAAga                        >  2:634230/1‑92 (MQ=255)
                                                                  gCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCa                       <  1:9743688/93‑1 (MQ=255)
                                                                  gCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCa                       >  1:7292755/1‑93 (MQ=255)
                                                                  gCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCa                       <  1:6321631/93‑1 (MQ=255)
                                                                  gCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCa                       <  1:5769364/93‑1 (MQ=255)
                                                                     gAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTg                    >  2:2557270/1‑93 (MQ=255)
                                                                      aaGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGa                   >  1:9137695/1‑93 (MQ=255)
                                                                       aGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGaa                  <  1:6047307/93‑1 (MQ=255)
                                                                       aGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGaa                  >  1:7840037/1‑93 (MQ=255)
                                                                        gtgtCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAAc                 <  2:2784493/93‑1 (MQ=255)
                                                                           tCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTg              >  2:1614060/1‑93 (MQ=255)
                                                                           tCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTg              >  1:9186809/1‑93 (MQ=255)
                                                                           tCCAGCCGTTTCAAGACCAACCCGAAAACGGTCGTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTg              <  1:9670276/93‑1 (MQ=255)
                                                                             cAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGtt            <  2:5187536/93‑1 (MQ=255)
                                                                              aGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTa           <  1:3643198/93‑1 (MQ=255)
                                                                               gCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTAc          <  1:5644649/93‑1 (MQ=255)
                                                                                ccGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACa         >  2:6910094/1‑93 (MQ=255)
                                                                                ccGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACa         <  2:5482822/93‑1 (MQ=255)
                                                                                 cGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACAt        <  1:6583498/93‑1 (MQ=255)
                                                                                   tttCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAg      >  2:1909503/1‑93 (MQ=255)
                                                                                   tttCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAg      >  1:1654083/1‑93 (MQ=255)
                                                                                   tttCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAg      >  2:3784549/1‑93 (MQ=255)
                                                                                    ttCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGt     <  1:8822456/93‑1 (MQ=255)
                                                                                    ttCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGt     <  1:3981305/93‑1 (MQ=255)
                                                                                     tCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTg    <  2:9152282/93‑1 (MQ=255)
                                                                                     tCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTg    >  2:66395/1‑93 (MQ=255)
                                                                                       aaGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCg  >  2:7506173/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCG  >  NC_000913/775282‑775461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTG  >  NC_000913/775279‑775465
                                                                                             |                                                                                             
GGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGA                                                                                               >  SRR4302540.7208006/1‑94 (MQ=60)
 GTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGAGGAAGTGTCCAGCCGTTTCAAGAC                                                                                              >  SRR4302540.5163020/1‑94 (MQ=60)
  TATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACC                                                                                             >  SRR4302540.4572374/1‑94 (MQ=60)
  TATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACC                                                                                             <  SRR4302540.4896391/94‑1 (MQ=60)
  TATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACC                                                                                             >  SRR4302540.530683/1‑94 (MQ=60)
   ATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCA                                                                                            <  SRR4302540.4140446/94‑1 (MQ=60)
   ATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCA                                                                                            >  SRR4302540.4917133/1‑94 (MQ=60)
   ATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCA                                                                                            >  SRR4302540.4917134/1‑94 (MQ=60)
    TTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAA                                                                                           <  SRR4302540.9737966/94‑1 (MQ=60)
    TTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAA                                                                                           <  SRR4302540.4420129/94‑1 (MQ=60)
     TGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAAC                                                                                          <  SRR4302540.3111022/94‑1 (MQ=60)
      GGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACC                                                                                         >  SRR4302540.8420171/1‑94 (MQ=60)
      GGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACC                                                                                         >  SRR4302540.3462967/1‑94 (MQ=60)
       GTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCC                                                                                        <  SRR4302540.5929741/94‑1 (MQ=60)
       GTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCC                                                                                        >  SRR4302540.8543658/1‑94 (MQ=60)
       GTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCC                                                                                        <  SRR4302540.4230037/94‑1 (MQ=60)
        GCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCG                                                                                       <  SRR4302540.2742211/94‑1 (MQ=60)
          AGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAA                                                                                     <  SRR4302540.2878569/94‑1 (MQ=60)
            TACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAA                                                                                   >  SRR4302540.5575458/1‑94 (MQ=60)
            TACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAA                                                                                   >  SRR4302540.412802/1‑94 (MQ=60)
             ACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAAC                                                                                  >  SRR4302540.1699804/1‑94 (MQ=60)
             ACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAAC                                                                                  >  SRR4302540.4580525/1‑94 (MQ=60)
             ACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAAC                                                                                  >  SRR4302540.9296849/1‑94 (MQ=60)
             ACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAAC                                                                                  <  SRR4302540.9653955/94‑1 (MQ=60)
              CACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACG                                                                                 >  SRR4302540.9998938/1‑94 (MQ=60)
               ACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGG                                                                                >  SRR4302540.5282219/1‑94 (MQ=60)
               ACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTAAAGACCAACCCGAAAACGG                                                                                >  SRR4302540.4770324/1‑94 (MQ=60)
                 CGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGATGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTC                                                                              >  SRR4302540.9952739/1‑94 (MQ=60)
                  GTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCT                                                                             >  SRR4302540.8143784/1‑94 (MQ=60)
                  GTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCT                                                                             >  SRR4302540.9239920/1‑94 (MQ=60)
                    GGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTT                                                                           <  SRR4302540.1973806/94‑1 (MQ=60)
                    GGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTT                                                                           >  SRR4302540.3924206/1‑94 (MQ=60)
                    GGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTT                                                                           >  SRR4302540.3924205/1‑94 (MQ=60)
                     GTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTC                                                                          >  SRR4302540.5086394/1‑94 (MQ=60)
                      TGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCT                                                                         >  SRR4302540.5974825/1‑94 (MQ=60)
                      TGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCT                                                                         >  SRR4302540.1390542/1‑94 (MQ=60)
                       GGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTG                                                                        >  SRR4302540.9896390/1‑94 (MQ=60)
                       GGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTG                                                                        <  SRR4302540.2956035/94‑1 (MQ=60)
                       GGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTG                                                                        >  SRR4302540.3863568/1‑94 (MQ=60)
                       GGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGGGTCCAGCCGGTTCAAGACCAACCCGAAAACGGTCTTTCTG                                                                        >  SRR4302540.2064650/1‑94 (MQ=60)
                        GTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGA                                                                       >  SRR4302540.9936469/1‑94 (MQ=60)
                        GTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGA                                                                       >  SRR4302540.3087575/1‑94 (MQ=60)
                         TTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGAT                                                                      <  SRR4302540.2971233/94‑1 (MQ=60)
                         TTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGAT                                                                      <  SRR4302540.9277569/94‑1 (MQ=60)
                         TTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGAT                                                                      >  SRR4302540.3608459/1‑94 (MQ=60)
                         TTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGAT                                                                      <  SRR4302540.1349975/94‑1 (MQ=60)
                         TTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCATTTCAAGACCAACCCGAAAACGGTCTTTCTGAT                                                                      >  SRR4302540.3367956/1‑94 (MQ=60)
                          TGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATC                                                                     >  SRR4302540.7250024/1‑94 (MQ=60)
                          TGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATC                                                                     >  SRR4302540.4702834/1‑94 (MQ=60)
                          TGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATC                                                                     >  SRR4302540.5608431/1‑94 (MQ=60)
                           GAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCG                                                                    <  SRR4302540.7653569/94‑1 (MQ=60)
                           GAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCG                                                                    <  SRR4302540.6292982/94‑1 (MQ=60)
                            AGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGG                                                                   <  SRR4302540.5149061/94‑1 (MQ=60)
                            AGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGG                                                                   >  SRR4302540.2019646/1‑94 (MQ=60)
                             GAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGT                                                                  >  SRR4302540.129666/1‑94 (MQ=60)
                             GAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGT                                                                  <  SRR4302540.4786192/94‑1 (MQ=60)
                              AAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTTGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTG                                                                 >  SRR4302540.5060776/1‑94 (MQ=60)
                              AAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTG                                                                 <  SRR4302540.2717151/94‑1 (MQ=60)
                               AAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGG                                                                >  SRR4302540.9516236/1‑94 (MQ=60)
                               AAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGG                                                                >  SRR4302540.2843035/1‑94 (MQ=60)
                                 GATCGGCTGGAGCGTTTACCACCAGAGCAGTTGGTGGCAGAAGTGTCCAGCCGTTTCAAGACCACCCCGAAAACGGTCTTTCTGATCGGTGGCG                                                              <  SRR4302540.239599/94‑1 (MQ=60)
                                   TCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCA                                                            >  SRR4302540.6042833/1‑94 (MQ=60)
                                    CGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAA                                                           <  SRR4302540.4440662/94‑1 (MQ=60)
                                    CGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAA                                                           <  SRR4302540.4440663/94‑1 (MQ=60)
                                     GCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAA                                                          >  SRR4302540.3170680/1‑94 (MQ=60)
                                     GCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAA                                                          >  SRR4302540.3655643/1‑94 (MQ=60)
                                      CCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAA                                                         >  SRR4302540.8371206/1‑94 (MQ=60)
                                      CCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAA                                                         >  SRR4302540.8743299/1‑94 (MQ=60)
                                      CCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAA                                                         >  SRR4302540.8370945/1‑94 (MQ=60)
                                      CCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGCCTTTCTGATCGGTGGCGCAAAA                                                         >  SRR4302540.7260756/1‑94 (MQ=60)
                                       CTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAG                                                        >  SRR4302540.47710/1‑94 (MQ=60)
                                       CTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAG                                                        >  SRR4302540.3170725/1‑94 (MQ=60)
                                       CTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAG                                                        >  SRR4302540.725666/1‑94 (MQ=60)
                                       CTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAG                                                        >  SRR4302540.2524507/1‑94 (MQ=60)
                                       CTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACAGTCTTTCTGATCGGTAGAGCAAAAG                                                        >  SRR4302540.5679636/1‑94 (MQ=60)
                                        TGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGA                                                       >  SRR4302540.9579240/1‑94 (MQ=60)
                                         GGAGCGTTTACCACCAGAGCAGGTGGTGGCGGATGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAT                                                      <  SRR4302540.9806659/94‑1 (MQ=60)
                                         GGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAT                                                      >  SRR4302540.6801003/1‑94 (MQ=60)
                                         GGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAT                                                      >  SRR4302540.5926401/1‑94 (MQ=60)
                                          GGGCGTTTACCTCCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATG                                                     <  SRR4302540.8332162/94‑1 (MQ=60)
                                          GAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATG                                                     <  SRR4302540.9335015/94‑1 (MQ=60)
                                          GAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATG                                                     >  SRR4302540.282677/1‑94 (MQ=60)
                                           AGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGT                                                    <  SRR4302540.4854457/94‑1 (MQ=60)
                                           AGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGT                                                    <  SRR4302540.3721804/94‑1 (MQ=60)
                                           AGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGAGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGT                                                    <  SRR4302540.9845971/94‑1 (MQ=60)
                                            GCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTG                                                   >  SRR4302540.6538938/1‑94 (MQ=60)
                                            GCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTG                                                   <  SRR4302540.1302017/94‑1 (MQ=60)
                                             CGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGC                                                  >  SRR4302540.8172257/1‑94 (MQ=60)
                                             CGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGC                                                  <  SRR4302540.7928766/94‑1 (MQ=60)
                                              GTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCC                                                 >  SRR4302540.4698402/1‑94 (MQ=60)
                                              GTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCC                                                 >  SRR4302540.2540247/1‑94 (MQ=60)
                                               TTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCT                                                <  SRR4302540.8128703/94‑1 (MQ=60)
                                               TTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCT                                                >  SRR4302540.2303735/1‑94 (MQ=60)
                                               TTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCT                                                <  SRR4302540.9381699/94‑1 (MQ=60)
                                                TTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTT                                               >  SRR4302540.4948661/1‑94 (MQ=60)
                                                TTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTT                                               >  SRR4302540.4948658/1‑94 (MQ=60)
                                                 TACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTA                                              <  SRR4302540.7020160/94‑1 (MQ=60)
                                                 TACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTA                                              <  SRR4302540.7974993/94‑1 (MQ=60)
                                                   CCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACG                                            <  SRR4302540.9360337/94‑1 (MQ=60)
                                                     ACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGAT                                          >  SRR4302540.5777496/1‑94 (MQ=60)
                                                     ACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGAT                                          >  SRR4302540.6664493/1‑94 (MQ=60)
                                                     ACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGAT                                          >  SRR4302540.6745569/1‑94 (MQ=60)
                                                     ACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGAT                                          >  SRR4302540.9361559/1‑94 (MQ=60)
                                                       CAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGGCTTACGATGA                                        >  SRR4302540.625059/1‑94 (MQ=60)
                                                       CAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGA                                        >  SRR4302540.9750603/1‑94 (MQ=60)
                                                       CAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGA                                        >  SRR4302540.2139110/1‑94 (MQ=60)
                                                        AGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAA                                       >  SRR4302540.2187621/1‑94 (MQ=60)
                                                        AGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAA                                       >  SRR4302540.9228098/1‑94 (MQ=60)
                                                        AGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAA                                       >  SRR4302540.7194987/1‑94 (MQ=60)
                                                        AGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAA                                       >  SRR4302540.3833097/1‑94 (MQ=60)
                                                         GAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAA                                      >  SRR4302540.1347617/1‑94 (MQ=60)
                                                          AGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAAT                                     <  SRR4302540.3857547/94‑1 (MQ=60)
                                                            CAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAA                                   <  SRR4302540.866643/94‑1 (MQ=60)
                                                            CAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAA                                   >  SRR4302540.313319/1‑94 (MQ=60)
                                                            CAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAA                                   <  SRR4302540.4590866/94‑1 (MQ=60)
                                                            CAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAA                                   <  SRR4302540.9510151/94‑1 (MQ=60)
                                                            CAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACAAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGTGGTGCTTTAGGATGAAATAT                                   >  SRR4302540.2143817/1‑94 (MQ=60)
                                                             AGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAAT                                  >  SRR4302540.9370107/1‑94 (MQ=60)
                                                             AGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGGCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAAT                                  >  SRR4302540.3337631/1‑94 (MQ=60)
                                                              GGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATT                                 >  SRR4302540.2212588/1‑94 (MQ=60)
                                                              GGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATT                                 >  SRR4302540.7577385/1‑94 (MQ=60)
                                                              GGTGGTGGCGGAAGTGTCCAGCCGGTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGCTGTGCCTTACGATGAAATAATT                                 <  SRR4302540.1851663/94‑1 (MQ=60)
                                                              GGGGGGGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATT                                 <  SRR4302540.1851662/94‑1 (MQ=60)
                                                               GTGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTA                                <  SRR4302540.4660265/94‑1 (MQ=60)
                                                                TGGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAA                               >  SRR4302540.7832982/1‑94 (MQ=60)
                                                                 GGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAA                              <  SRR4302540.6882924/94‑1 (MQ=60)
                                                                 GGTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAA                              <  SRR4302540.5517380/94‑1 (MQ=60)
                                                                  GTGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAG                             <  SRR4302540.9310525/94‑1 (MQ=60)
                                                                   TGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGC                            <  SRR4302540.91867/94‑1 (MQ=60)
                                                                   TGGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGC                            >  SRR4302540.8512737/1‑94 (MQ=60)
                                                                    GGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCA                           <  SRR4302540.5478538/94‑1 (MQ=60)
                                                                    GGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCA                           <  SRR4302540.9960115/94‑1 (MQ=60)
                                                                    GGCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCA                           >  SRR4302540.7374139/1‑94 (MQ=60)
                                                                     GCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCAC                          <  SRR4302540.6392028/94‑1 (MQ=60)
                                                                     GCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCAC                          <  SRR4302540.5833084/94‑1 (MQ=60)
                                                                     GCGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCAC                          <  SRR4302540.9858048/94‑1 (MQ=60)
                                                                     GCGAAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCAC                          <  SRR4302540.8015467/94‑1 (MQ=60)
                                                                      CGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACT                         <  SRR4302540.7052789/94‑1 (MQ=60)
                                                                      CGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACT                         <  SRR4302540.4795603/94‑1 (MQ=60)
                                                                      CGGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGCTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACT                         <  SRR4302540.3991707/94‑1 (MQ=60)
                                                                       GGAAGTGTCCAGCCGTTTCAAGACCAGCCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTG                        <  SRR4302540.5918611/94‑1 (MQ=60)
                                                                       GGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTG                        >  SRR4302540.5004772/1‑94 (MQ=60)
                                                                       GGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTG                        >  SRR4302540.1873840/1‑94 (MQ=60)
                                                                       GGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTG                        >  SRR4302540.404973/1‑94 (MQ=60)
                                                                       GGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTG                        >  SRR4302540.3619780/1‑94 (MQ=60)
                                                                       GGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTA                        >  SRR4302540.7436266/1‑94 (MQ=60)
                                                                       GGAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGAACTG                        >  SRR4302540.5155808/1‑94 (MQ=60)
                                                                        GAAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGA                       >  SRR4302540.9247492/1‑94 (MQ=60)
                                                                         AAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAA                      >  SRR4302540.10101140/1‑94 (MQ=60)
                                                                         AAGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAA                      >  SRR4302540.7931638/1‑94 (MQ=60)
                                                                          AGTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAAC                     <  SRR4302540.6114495/94‑1 (MQ=60)
                                                                           GTGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACT                    >  SRR4302540.7015935/1‑94 (MQ=60)
                                                                            TGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTT                   >  SRR4302540.2166653/1‑94 (MQ=60)
                                                                            TGTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTT                   <  SRR4302540.4321354/94‑1 (MQ=60)
                                                                             GTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTG                  <  SRR4302540.3914475/94‑1 (MQ=60)
                                                                             GTCCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTG                  >  SRR4302540.9296982/1‑94 (MQ=60)
                                                                              TCCAGCCGTTTCAAGACCAACCCGAAAACGGTCGTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGT                 <  SRR4302540.9784085/94‑1 (MQ=60)
                                                                               CCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTT                >  SRR4302540.3676096/1‑94 (MQ=60)
                                                                               CCAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTT                >  SRR4302540.6591103/1‑94 (MQ=60)
                                                                                CAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTA               >  SRR4302540.6994395/1‑94 (MQ=60)
                                                                                CAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTA               >  SRR4302540.2506192/1‑94 (MQ=60)
                                                                                CAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTA               >  SRR4302540.6045831/1‑94 (MQ=60)
                                                                                CAGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTA               <  SRR4302540.6655450/94‑1 (MQ=60)
                                                                                 AGCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTAC              <  SRR4302540.3683099/94‑1 (MQ=60)
                                                                                  GCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACA             >  SRR4302540.1635350/1‑94 (MQ=60)
                                                                                  GCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACA             <  SRR4302540.5706776/94‑1 (MQ=60)
                                                                                  GCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACA             <  SRR4302540.4892599/94‑1 (MQ=60)
                                                                                  GCCGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACA             <  SRR4302540.5706775/94‑1 (MQ=60)
                                                                                    CGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATA           <  SRR4302540.6656420/94‑1 (MQ=60)
                                                                                    CGTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATA           >  SRR4302540.9666522/1‑94 (MQ=60)
                                                                                     GTTTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAG          >  SRR4302540.1672105/1‑94 (MQ=60)
                                                                                       TTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTG        <  SRR4302540.8924472/94‑1 (MQ=60)
                                                                                       TTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTG        >  SRR4302540.5350006/1‑94 (MQ=60)
                                                                                       TTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTG        <  SRR4302540.4024185/94‑1 (MQ=60)
                                                                                       TTCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTG        >  SRR4302540.7162979/1‑94 (MQ=60)
                                                                                        TCAAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGC       >  SRR4302540.37241/1‑94 (MQ=60)
                                                                                          AAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGG     >  SRR4302540.3475956/1‑94 (MQ=60)
                                                                                          AAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGG     >  SRR4302540.9429421/1‑94 (MQ=60)
                                                                                          AAGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGG     >  SRR4302540.2926089/1‑94 (MQ=60)
                                                                                           AGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGG    >  SRR4302540.2400585/1‑94 (MQ=60)
                                                                                           AGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGG    >  SRR4302540.6623265/1‑94 (MQ=60)
                                                                                           AGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGG    <  SRR4302540.316957/94‑1 (MQ=60)
                                                                                           AGACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGG    >  SRR4302540.6623267/1‑94 (MQ=60)
                                                                                             ACCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTG  >  SRR4302540.2962935/1‑94 (MQ=60)
                                                                                             |                                                                                             
GGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTG  >  NC_000913/775279‑775465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑