Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	780,533	A→G	intergenic (+88/‑59)	lysY → / → lysZ	tRNA‑Lys/tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	780,533	0	A	G	99.5%	686.0 / NA	217	intergenic (+88/‑59)	lysY/lysZ	tRNA‑Lys/tRNA‑Lys
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (113/103); minor base C (1/0); total (114/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

CCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGA  >  NC_000913/780443‑780622

  aGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGAc                                                                                       <  1:341886/93‑1 (MQ=255)

    ttttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGAccc                                                                                     <  2:648861/93‑1 (MQ=255)

      ttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACccac                                                                                   <  2:2709265/93‑1 (MQ=255)

       tAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACccacc                                                                                  <  2:1257979/93‑1 (MQ=255)

       tAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACccacc                                                                                  >  2:2558073/1‑93 (MQ=255)

       tAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACccacc                                                                                  >  2:3074558/1‑93 (MQ=255)

         aCATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCaa                                                                                >  2:3101477/1‑93 (MQ=255)

          cATCAAACTCAGCTGTTAAGCGTGAAGGATAACGTTGCGCCAGCAGCGCCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAAt                                                                               <  2:158274/93‑1 (MQ=17)

           aTCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATc                                                                              <  1:1914405/93‑1 (MQ=255)

           aTCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATc                                                                              <  2:4590166/93‑1 (MQ=255)

            tCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCt                                                                             >  2:4121206/1‑93 (MQ=255)

             cAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCtt                                                                            >  2:1837032/1‑93 (MQ=255)

              aaaCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTa                                                                           >  1:1828284/1‑93 (MQ=255)

               aaCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTaa                                                                          >  2:788708/1‑93 (MQ=255)

               aaCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTaa                                                                          >  2:4741727/1‑93 (MQ=255)

               aaCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTaa                                                                          >  2:684112/1‑93 (MQ=255)

                aCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTaaa                                                                         <  2:5674936/93‑1 (MQ=255)

                aCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTaaa                                                                         >  2:1437524/1‑93 (MQ=255)

                aCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTaaa                                                                         <  1:3707221/93‑1 (MQ=255)

                 cTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAg                                                                        >  1:3047092/1‑93 (MQ=255)

                  tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGa                                                                       >  1:2807604/1‑93 (MQ=255)

                  tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGGCATCCTGCGCGACCCACCAATCTTAAAGa                                                                       >  2:2442486/1‑93 (MQ=255)

                   cAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGAt                                                                      >  1:2290876/1‑93 (MQ=255)

                     gATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTg                                                                    <  1:107141/93‑1 (MQ=255)

                     gATGTTAAGCGTGAAGGATAACGTTGCGCCAGAAACGGCCCGTAGGGCGAGCGAAGCGGGTCATACTGCGCGACCCACCAATCTTAGAGATTg                                                                    >  2:2775892/1‑93 (MQ=17)

                      aTGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTgg                                                                   <  2:4071640/93‑1 (MQ=255)

                      aTGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTgg                                                                   <  1:2944888/93‑1 (MQ=255)

                       tGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTgtc                                                                  >  1:1673388/1‑91 (MQ=255)

                       tGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGc                                                                  >  1:2532267/1‑93 (MQ=255)

                       tGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGc                                                                  <  2:547346/93‑1 (MQ=255)

                         ttAATCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGACCGAAGCGAGTCATCCTGCGGGACCCACCAATCTTAAAGATTGGccc                                                                <  2:2098062/93‑1 (MQ=18)

                         ttAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGccc                                                                >  2:4060586/1‑93 (MQ=255)

                         ttAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGccc                                                                >  2:2278276/1‑93 (MQ=255)

                          tAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGcccc                                                               <  2:606972/93‑1 (MQ=255)

                               gTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTa                                                          >  2:1429202/1‑93 (MQ=255)

                               gTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTa                                                          >  1:5578992/1‑93 (MQ=255)

                                tGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTaa                                                         >  2:1173377/1‑93 (MQ=255)

                                   aGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTaaaaa                                                      <  1:3507391/93‑1 (MQ=255)

                                    ggATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAAt                                                     <  2:4994226/93‑1 (MQ=255)

                                    ggATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAAt                                                     >  1:2571115/1‑93 (MQ=255)

                                     gATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATc                                                    <  1:396687/93‑1 (MQ=255)

                                     gATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATc                                                    <  2:542814/93‑1 (MQ=255)

                                      aTAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCt                                                   >  2:2175648/1‑93 (MQ=255)

                                      aTAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCt                                                   <  2:5919231/93‑1 (MQ=255)

                                      aTAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCt                                                   >  1:3313940/1‑93 (MQ=255)

                                      aTAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCt                                                   >  2:6117014/1‑93 (MQ=255)

                                       tAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCtt                                                  <  2:1283683/93‑1 (MQ=255)

                                        aaCGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCttc                                                 >  1:5068130/1‑92 (MQ=255)

                                         aCGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTc                                                <  1:6076827/93‑1 (MQ=255)

                                         aCGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTc                                                >  1:4942530/1‑93 (MQ=255)

                                         aCGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTc                                                >  1:6298923/1‑93 (MQ=255)

                                         aCGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTc                                                <  2:2982506/93‑1 (MQ=255)

                                          cGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                               <  1:5764892/93‑1 (MQ=255)

                                           gTTGCGCCAGCAACGTCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAg                                              <  1:2580507/93‑1 (MQ=255)

                                            ttGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAgg                                             >  2:291077/1‑93 (MQ=255)

                                               cgcCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTaa                                          >  2:5521223/1‑93 (MQ=255)

                                               cgcCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTaa                                          <  1:1567496/93‑1 (MQ=255)

                                                 ccAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAaca                                        >  2:1047262/1‑93 (MQ=255)

                                                 ccAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAaca                                        >  1:1222644/1‑93 (MQ=255)

                                                 ccAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAaca                                        <  1:4248155/93‑1 (MQ=255)

                                                      aaCGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGt                                   <  1:4651520/93‑1 (MQ=255)

                                                      aaCGGCCCGTAGGGCGAGCGAAGCGAGGCATCCTGCGCGACCCACCAATCTTAAAGATTAGGCCCGAGTAAAAATCTTTCAGGTAACACCCGt                                   >  2:5736584/1‑93 (MQ=25)

                                                       aCGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTa                                  >  1:90633/1‑93 (MQ=255)

                                                       aCGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTa                                  <  2:259115/93‑1 (MQ=255)

                                                       aCGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTa                                  >  2:5186878/1‑93 (MQ=255)

                                                        cGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTAt                                 <  2:4533689/93‑1 (MQ=255)

                                                          gCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGACCCCGAGTAAAAATCTTTCAGGTAACACCCGTATgg                               >  2:3512004/1‑93 (MQ=255)

                                                             cGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTc                            <  1:2993476/93‑1 (MQ=255)

                                                             cGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTc                            <  2:6186748/93‑1 (MQ=255)

                                                               tAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGt                          >  2:3474058/1‑93 (MQ=255)

                                                                aGGGCGAGCGAAGCTAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGtt                         <  2:1531784/93‑1 (MQ=255)

                                                                 gggCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTa                        <  2:4192245/93‑1 (MQ=255)

                                                                 gggCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTa                        <  1:4158127/93‑1 (MQ=255)

                                                                  ggCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAg                       >  2:4673361/1‑93 (MQ=255)

                                                                  ggCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAg                       >  1:2013124/1‑93 (MQ=255)

                                                                    cgagcgaAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCt                     >  1:103854/1‑93 (MQ=255)

                                                                    cgagcgaAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCt                     >  1:3845295/1‑93 (MQ=255)

                                                                    cgagcgaAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCt                     <  1:3853713/93‑1 (MQ=255)

                                                                        cgaagcgaGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCATTAGCTCAGt                 <  2:4842131/93‑1 (MQ=255)

                                                                            gcgaGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGt             >  2:3158674/1‑93 (MQ=255)

                                                                              gaGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTag           <  1:2103664/93‑1 (MQ=255)

                                                                              gaGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTag           >  1:2776136/1‑93 (MQ=255)

                                                                              gaGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTag           <  2:5169850/93‑1 (MQ=255)

                                                                              gaGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTag           >  2:5283940/1‑93 (MQ=255)

                                                                              gaGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTag           <  1:4781583/93‑1 (MQ=255)

                                                                              gaGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTag           >  1:543038/1‑93 (MQ=255)

                                                                               aGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTaga          >  1:4218684/1‑93 (MQ=255)

                                                                                gTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTagag         >  1:2644168/1‑93 (MQ=255)

                                                                                gTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTagag         >  1:743413/1‑93 (MQ=255)

                                                                                 tCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGc        <  1:3133798/93‑1 (MQ=255)

                                                                                 tCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGc        <  1:4060931/93‑1 (MQ=255)

                                                                                 tCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGc        <  1:537854/93‑1 (MQ=255)

                                                                                  cATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCa       <  2:1692178/93‑1 (MQ=255)

                                                                                   aTCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAg      >  2:2014298/1‑93 (MQ=255)

                                                                                   aTCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAg      >  2:3737541/1‑93 (MQ=255)

                                                                                    tCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGt     <  1:6263245/93‑1 (MQ=255)

                                                                                    tCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGt     >  2:4012782/1‑93 (MQ=255)

                                                                                    tCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGt     >  2:4224171/1‑93 (MQ=255)

                                                                                     ccTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGtt    <  1:319298/93‑1 (MQ=255)

                                                                                       tGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGa  <  1:4873158/93‑1 (MQ=255)

CCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGA  >  NC_000913/780443‑780622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTT  >  NC_000913/780440‑780625

CCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCG                                                                                              >  SRR4302533.2726685/1‑94 (MQ=60)

 CACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGC                                                                                             >  SRR4302533.2054880/1‑94 (MQ=60)

 CACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGC                                                                                             <  SRR4302533.3232033/94‑1 (MQ=60)

   CCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGA                                                                                           <  SRR4302533.3504470/94‑1 (MQ=60)

    CAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGAC                                                                                          >  SRR4302533.4615068/1‑94 (MQ=60)

    CAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGAC                                                                                          >  SRR4302533.2164015/1‑94 (MQ=60)

     AGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACC                                                                                         <  SRR4302533.343974/94‑1 (MQ=60)

       TTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCA                                                                                       >  SRR4302533.4198615/1‑94 (MQ=60)

       TTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCA                                                                                       >  SRR4302533.2575795/1‑94 (MQ=60)

           AACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAA                                                                                   >  SRR4302533.3871642/1‑94 (MQ=60)

           AACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAA                                                                                   >  SRR4302533.1878848/1‑94 (MQ=60)

           AACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAA                                                                                   >  SRR4302533.2431772/1‑94 (MQ=60)

              ATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCT                                                                                <  SRR4302533.1827374/94‑1 (MQ=55)

              ATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCT                                                                                <  SRR4302533.1926090/94‑1 (MQ=55)

                CAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTA                                                                              >  SRR4302533.1839449/1‑94 (MQ=55)

                   ACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAG                                                                           <  SRR4302533.3729990/94‑1 (MQ=55)

                   ACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAG                                                                           >  SRR4302533.3065747/1‑94 (MQ=55)

                    CTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGA                                                                          >  SRR4302533.2824882/1‑94 (MQ=55)

                     TCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGAT                                                                         >  SRR4302533.2305063/1‑94 (MQ=55)

                     TCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGAT                                                                         >  SRR4302533.963944/1‑94 (MQ=55)

                      CAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATT                                                                        >  SRR4302533.4052976/1‑94 (MQ=55)

                       AGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTG                                                                       >  SRR4302533.3650860/1‑94 (MQ=58)

                        GATGTTAAGCGTTAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGG                                                                      <  SRR4302533.4918635/94‑1 (MQ=58)

                        GATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGG                                                                      <  SRR4302533.107813/94‑1 (MQ=60)

                        GATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGG                                                                      <  SRR4302533.3339045/94‑1 (MQ=60)

                         ATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGTC                                                                     >  SRR4302533.1683501/1‑94 (MQ=57)

                         ATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGC                                                                     >  SRR4302533.2547855/1‑94 (MQ=60)

                         ATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGC                                                                     <  SRR4302533.4901818/94‑1 (MQ=60)

                         ATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGC                                                                     <  SRR4302533.2962902/94‑1 (MQ=60)

                           GTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCC                                                                   <  SRR4302533.3834977/94‑1 (MQ=60)

                             TAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCG                                                                 >  SRR4302533.3121958/1‑94 (MQ=60)

                                 CGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTA                                                             >  SRR4302533.5612709/1‑94 (MQ=60)

                                 CGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTA                                                             >  SRR4302533.4485234/1‑94 (MQ=60)

                                 CGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTA                                                             >  SRR4302533.5148956/1‑94 (MQ=60)

                                  GTGAAGGATAACGTTGCGCCAGCAACGGACCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAA                                                            >  SRR4302533.2950683/1‑94 (MQ=60)

                                    GAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAA                                                          >  SRR4302533.2715776/1‑94 (MQ=60)

                                      AGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAAT                                                        <  SRR4302533.3529020/94‑1 (MQ=60)

                                      AGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAAT                                                        >  SRR4302533.2586937/1‑94 (MQ=60)

                                       GGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATC                                                       <  SRR4302533.399460/94‑1 (MQ=60)

                                        GATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCT                                                      <  SRR4302533.1683476/94‑1 (MQ=60)

                                        GATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCT                                                      >  SRR4302533.3334401/1‑94 (MQ=60)

                                        GATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCT                                                      <  SRR4302533.399087/94‑1 (MQ=60)

                                          TAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTT                                                    <  SRR4302533.4255389/94‑1 (MQ=60)

                                          TAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTT                                                    >  SRR4302533.410397/1‑94 (MQ=60)

                                          TAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTC                                                    >  SRR4302533.5098735/1‑94 (MQ=60)

                                           AACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTC                                                   >  SRR4302533.4972448/1‑94 (MQ=60)

                                           AACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTC                                                   >  SRR4302533.6336526/1‑94 (MQ=60)

                                           AACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTC                                                   <  SRR4302533.5165757/94‑1 (MQ=60)

                                            ACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCA                                                  <  SRR4302533.6113579/94‑1 (MQ=60)

                                             CGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAG                                                 <  SRR4302533.5799681/94‑1 (MQ=60)

                                              GTTGCGCCAGCAACGTCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGG                                                <  SRR4302533.2596386/94‑1 (MQ=60)

                                                  CGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAAC                                            <  SRR4302533.1576937/94‑1 (MQ=60)

                                                   GCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACA                                           >  SRR4302533.1229975/1‑94 (MQ=60)

                                                    CCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACAC                                          <  SRR4302533.4998534/94‑1 (MQ=60)

                                                    CCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACAC                                          <  SRR4302533.4273795/94‑1 (MQ=60)

                                                       GCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCG                                       <  SRR4302533.1048808/94‑1 (MQ=60)

                                                        CAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACTCACCAATATTAAAGATTGGACCCGAGTAAAAATCTTTCAGGTAACACCCGT                                      >  SRR4302533.3489606/1‑94 (MQ=60)

                                                         AACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTA                                     >  SRR4302533.5687659/1‑94 (MQ=60)

                                                         AACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTA                                     <  SRR4302533.4679705/94‑1 (MQ=60)

                                                         AACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTA                                     >  SRR4302533.91206/1‑94 (MQ=60)

                                                         AACGGCCCGTAGGGAGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTA                                     >  SRR4302533.3983394/1‑94 (MQ=60)

                                                          ACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTAT                                    <  SRR4302533.320173/94‑1 (MQ=60)

                                                           CGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATG                                   <  SRR4302533.1098251/94‑1 (MQ=60)

                                                           CGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATG                                   <  SRR4302533.5412203/94‑1 (MQ=60)

                                                            GGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGG                                  <  SRR4302533.854378/94‑1 (MQ=60)

                                                             GCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGG                                 <  SRR4302533.3305847/94‑1 (MQ=60)

                                                               CCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTC                               >  SRR4302533.6028409/1‑94 (MQ=60)

                                                               CCGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTC                               <  SRR4302533.2817072/94‑1 (MQ=60)

                                                                CGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCG                              <  SRR4302533.3011779/94‑1 (MQ=60)

                                                                CGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCG                              <  SRR4302533.2024911/94‑1 (MQ=60)

                                                                CGTAGGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCG                              >  SRR4302533.2588047/1‑94 (MQ=60)

                                                                    GGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAG                          <  SRR4302533.4183323/94‑1 (MQ=60)

                                                                    GGGCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAG                          >  SRR4302533.2025523/1‑94 (MQ=60)

                                                                      GCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCT                        >  SRR4302533.104504/1‑94 (MQ=60)

                                                                      GCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCT                        <  SRR4302533.1805403/94‑1 (MQ=60)

                                                                      GCGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCT                        >  SRR4302533.3868780/1‑94 (MQ=60)

                                                                       cgagggagaaGAGTCATCCTCCGCGACCCACCAAACTCAAAGATTGGCCCCGAGAAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTC                       <  SRR4302533.1203328/84‑1 (MQ=60)

                                                                       CGAGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTC                       <  SRR4302533.3877252/94‑1 (MQ=60)

                                                                         AGCGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAG                     <  SRR4302533.302598/94‑1 (MQ=60)

                                                                           CGAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTT                   >  SRR4302533.2026739/1‑94 (MQ=60)

                                                                            GAAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTG                  <  SRR4302533.6314109/94‑1 (MQ=60)

                                                                             AAGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGG                 <  SRR4302533.253197/94‑1 (MQ=60)

                                                                              AGCGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGT                <  SRR4302533.5835228/94‑1 (MQ=60)

                                                                                CGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAG              <  SRR4302533.742533/94‑1 (MQ=60)

                                                                                CGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAG              >  SRR4302533.2793187/1‑94 (MQ=60)

                                                                                CGAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAG              >  SRR4302533.546261/1‑94 (MQ=60)

                                                                                 GAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGA             <  SRR4302533.4810553/94‑1 (MQ=60)

                                                                                 GAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGA             <  SRR4302533.2116633/94‑1 (MQ=60)

                                                                                 GAGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGA             >  SRR4302533.4244199/1‑94 (MQ=60)

                                                                                  AGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAG            >  SRR4302533.2660415/1‑94 (MQ=60)

                                                                                  AGTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAG            >  SRR4302533.747789/1‑94 (MQ=60)

                                                                                   GTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGC           <  SRR4302533.2623890/94‑1 (MQ=60)

                                                                                   GTCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGC           >  SRR4302533.4694913/1‑94 (MQ=60)

                                                                                    TCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCA          <  SRR4302533.541054/94‑1 (MQ=60)

                                                                                    TCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCA          <  SRR4302533.3153068/94‑1 (MQ=60)

                                                                                    TCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCA          <  SRR4302533.3083204/94‑1 (MQ=60)

                                                                                    TCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCA          >  SRR4302533.3005877/1‑94 (MQ=60)

                                                                                    TCATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCA          <  SRR4302533.4085652/94‑1 (MQ=60)

                                                                                      ATCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGT        >  SRR4302533.5580379/1‑94 (MQ=60)

                                                                                       TCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTT       <  SRR4302533.6288876/94‑1 (MQ=60)

                                                                                       TCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTT       <  SRR4302533.6300681/94‑1 (MQ=60)

                                                                                       TCCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTT       <  SRR4302533.1327430/94‑1 (MQ=60)

                                                                                        CCTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTG      <  SRR4302533.321255/94‑1 (MQ=60)

                                                                                         CTGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGA     >  SRR4302533.2693865/1‑94 (MQ=60)

                                                                                          TGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC    <  SRR4302533.4902685/94‑1 (MQ=60)

                                                                                          TGCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAC    >  SRR4302533.1210509/1‑94 (MQ=60)

                                                                                           GCGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACT   <  SRR4302533.5235584/94‑1 (MQ=60)

                                                                                            CGCGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTT  <  SRR4302533.4875395/94‑1 (MQ=60)

CCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTT  >  NC_000913/780440‑780625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`