Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 991,040 A→G N399S (AAC→AGC)  pepN → aminopeptidase N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913991,0400AG99.6% 816.1 / ‑5.0 254N399S (AAC→AGC) pepNaminopeptidase N
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (112/141);  total (112/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.20e-01

Only 100 of 261 total aligned reads displayed.

CATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGT  >  NC_000913/990951‑991129
                                                                                         |                                                                                         
cATTGAGATGAACAACTTCTACGCCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCtt                                                                                        <  1:1495208/93‑1 (MQ=255)
 aTTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTc                                                                                       >  2:3505914/1‑93 (MQ=255)
 aTTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTc                                                                                       <  1:5319669/93‑1 (MQ=255)
 aTTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCACGATCCACACCCTGCTTGGCGAAGAAAGCTTc                                                                                       >  1:4831251/1‑93 (MQ=255)
  ttGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTcc                                                                                      <  2:4006276/93‑1 (MQ=255)
   tGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGCTTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCa                                                                                     <  2:5133747/93‑1 (MQ=255)
   tGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCa                                                                                     <  1:3813706/93‑1 (MQ=255)
   tGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCa                                                                                     >  1:737332/1‑93 (MQ=255)
    gagaTGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAg                                                                                    <  2:713252/93‑1 (MQ=255)
     agaTGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGa                                                                                   <  1:4674482/93‑1 (MQ=255)
       aTGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGaaa                                                                                 >  2:18019/1‑93 (MQ=255)
       aTGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGaaa                                                                                 >  2:6085757/1‑93 (MQ=255)
        tGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAg                                                                                <  1:1333049/93‑1 (MQ=255)
        tGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAg                                                                                >  1:335798/1‑93 (MQ=255)
         gAACAACTTCTGCACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAgg                                                                               <  1:2760097/93‑1 (MQ=255)
         gAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAgg                                                                               <  2:3599188/93‑1 (MQ=255)
          aacaacTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAggg                                                                              <  2:5916445/93‑1 (MQ=255)
          aacaacTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAggg                                                                              >  1:3049612/1‑93 (MQ=255)
           acaacTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGa                                                                             >  2:1701346/1‑93 (MQ=255)
             aacTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATg                                                                           >  1:6131199/1‑93 (MQ=255)
             aacTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATg                                                                           >  1:1015067/1‑93 (MQ=255)
              acTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTTGCATGATACACACCCTGCTTGGCGACGAAAGCTTCCAGAAAGGGATGc                                                                          >  2:5660072/1‑93 (MQ=255)
              acTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGc                                                                          <  1:887525/93‑1 (MQ=255)
              acTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGc                                                                          <  2:2935884/93‑1 (MQ=255)
              acTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGc                                                                          >  2:1169731/1‑93 (MQ=255)
                 tCTACGCCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGc                                                                       <  2:2877262/93‑1 (MQ=255)
                 tCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGc                                                                       <  2:3462880/93‑1 (MQ=255)
                 tCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGc                                                                       <  1:5542578/93‑1 (MQ=255)
                   tACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCtt                                                                     >  1:1973701/1‑93 (MQ=255)
                      acCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTAt                                                                  <  2:4934768/93‑1 (MQ=255)
                      acCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTAt                                                                  >  1:311684/1‑93 (MQ=255)
                       cccTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTAtt                                                                 >  2:2180991/1‑93 (MQ=255)
                       cccTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTAtt                                                                 >  2:1585198/1‑93 (MQ=255)
                         cTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTc                                                               >  1:4478496/1‑93 (MQ=255)
                         cTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTc                                                               <  2:5260493/93‑1 (MQ=255)
                         cTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTc                                                               <  2:3662900/93‑1 (MQ=255)
                          tGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCAGACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCg                                                              <  2:6213859/93‑1 (MQ=255)
                          tGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCg                                                              >  2:4575282/1‑93 (MQ=255)
                           gACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGa                                                             >  1:3075621/1‑93 (MQ=255)
                           gACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGa                                                             <  2:5818432/93‑1 (MQ=255)
                            aCCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAg                                                            >  1:5288031/1‑93 (MQ=255)
                            aCCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAg                                                            <  1:99698/93‑1 (MQ=255)
                            aCCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAg                                                            >  1:1131957/1‑93 (MQ=255)
                             ccGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGc                                                           <  2:1222061/93‑1 (MQ=255)
                             ccGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGc                                                           >  1:649470/1‑93 (MQ=255)
                              cGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCATCTTTATTTCGAGCg                                                          <  2:1431122/93‑1 (MQ=255)
                              cGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCg                                                          >  1:5214518/1‑93 (MQ=255)
                                tttACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTc                                                        >  2:2760596/1‑93 (MQ=255)
                                   aCGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCatg                                                     >  2:1152960/1‑93 (MQ=255)
                                     gagaAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCatgat                                                   <  1:4524108/93‑1 (MQ=255)
                                      agaAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCatgatg                                                  >  1:1675523/1‑93 (MQ=255)
                                       gaAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATgg                                                 >  1:4955092/1‑93 (MQ=255)
                                       gaAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATgg                                                 >  2:1994030/1‑93 (MQ=255)
                                       gaAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATgg                                                 >  1:181050/1‑93 (MQ=255)
                                        aaGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGt                                                <  1:6045619/93‑1 (MQ=255)
                                         aGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTa                                               <  2:2079533/93‑1 (MQ=255)
                                         aGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTa                                               >  1:4305758/1‑93 (MQ=255)
                                         aGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTa                                               <  2:4829429/93‑1 (MQ=255)
                                               cgGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGccg                                         >  2:2709582/1‑91 (MQ=255)
                                                ggAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGc                                        >  1:165629/1‑93 (MQ=255)
                                                ggAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGc                                        <  2:3735774/93‑1 (MQ=255)
                                                  aaGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGa                                      <  2:5216855/93‑1 (MQ=255)
                                                  aaGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGa                                      <  2:5321312/93‑1 (MQ=255)
                                                   aGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGAc                                     >  2:1627642/1‑93 (MQ=255)
                                                      gATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCtg                                  >  1:5579329/1‑93 (MQ=255)
                                                       aTTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCtgt                                 >  1:569706/1‑93 (MQ=255)
                                                         tCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTga                               <  2:3043389/93‑1 (MQ=255)
                                                         tCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTga                               <  1:3939122/93‑1 (MQ=255)
                                                         tCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTga                               <  1:3843287/93‑1 (MQ=255)
                                                          cGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTgac                              <  2:4202890/93‑1 (MQ=255)
                                                            cATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTgacga                            <  1:5198970/93‑1 (MQ=255)
                                                             aTGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTgacgcc                           >  2:435785/1‑91 (MQ=255)
                                                             aTGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTgacgac                           >  2:1613456/1‑93 (MQ=255)
                                                             aTGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTgacgac                           <  2:423768/93‑1 (MQ=255)
                                                               gATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACtt                         <  2:3148251/93‑1 (MQ=255)
                                                                aTCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACttt                        >  1:2673120/1‑93 (MQ=255)
                                                                aTCCACACCCTGCTGGGCGAAGAAAGCTTCCAGAAAGAGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACttt                        <  2:2801550/93‑1 (MQ=255)
                                                                 tCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTtg                       >  1:3157964/1‑93 (MQ=255)
                                                                  ccACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTtgt                      <  1:1039807/93‑1 (MQ=255)
                                                                  ccACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTtgt                      <  1:2378259/93‑1 (MQ=255)
                                                                   cacaCCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTtgtg                     >  2:5417599/1‑93 (MQ=255)
                                                                    acacCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGc                    <  2:737332/93‑1 (MQ=255)
                                                                    acacCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGc                    <  2:2859688/93‑1 (MQ=255)
                                                                     cacCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCa                   >  1:4045182/1‑93 (MQ=255)
                                                                     cacCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCa                   >  1:5867607/1‑93 (MQ=255)
                                                                      acCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAg                  <  2:4065201/93‑1 (MQ=255)
                                                                      acCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAg                  >  1:3460624/1‑93 (MQ=255)
                                                                      acCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAg                  >  2:2529611/1‑93 (MQ=255)
                                                                         cTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCg               >  1:11838/1‑93 (MQ=255)
                                                                          tGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGa              <  1:3555199/93‑1 (MQ=255)
                                                                           gCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGAt             <  2:4809481/93‑1 (MQ=255)
                                                                               ggCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGaa         <  2:5474645/93‑1 (MQ=255)
                                                                               ggCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGaa         >  2:5576441/1‑93 (MQ=255)
                                                                               ggCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGaa         <  1:2961004/93‑1 (MQ=255)
                                                                               ggCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGaa         <  2:466128/93‑1 (MQ=255)
                                                                                 cGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGa       <  1:669641/93‑1 (MQ=255)
                                                                                 cGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGa       <  2:4502142/93‑1 (MQ=255)
                                                                                   aagaaAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATg     >  1:2276476/1‑93 (MQ=255)
                                                                                    agaaAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGc    >  2:5087726/1‑93 (MQ=255)
                                                                                      aaagCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGt  >  1:324628/1‑93 (MQ=255)
                                                                                         |                                                                                         
CATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGT  >  NC_000913/990951‑991129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCG  >  NC_000913/990947‑991131
                                                                                             |                                                                                           
TGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAG                                                                                             <  SRR4302533.1993352/94‑1 (MQ=60)
TGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAG                                                                                             <  SRR4302533.1596965/94‑1 (MQ=60)
  GTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCT                                                                                           <  SRR4302533.4103288/94‑1 (MQ=60)
  GTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCT                                                                                           <  SRR4302533.5296421/94‑1 (MQ=60)
   TCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTT                                                                                          >  SRR4302533.495615/1‑94 (MQ=60)
    CATTGAGATGAACAACTTCTACGCCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTC                                                                                         <  SRR4302533.1504218/94‑1 (MQ=60)
    CATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCACGATCCACACCCTGCTTGGCGAAGAAAGCTTC                                                                                         >  SRR4302533.4860531/1‑94 (MQ=60)
     ATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCC                                                                                        <  SRR4302533.5351551/94‑1 (MQ=60)
      TTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCA                                                                                       >  SRR4302533.741673/1‑94 (MQ=60)
      TTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCA                                                                                       <  SRR4302533.4158335/94‑1 (MQ=60)
       TGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAG                                                                                      <  SRR4302533.5597361/94‑1 (MQ=60)
       TGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAG                                                                                      >  SRR4302533.5332292/1‑94 (MQ=60)
       TGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAG                                                                                      <  SRR4302533.3837028/94‑1 (MQ=60)
         AGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAA                                                                                    <  SRR4302533.4702806/94‑1 (MQ=60)
           ATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAG                                                                                  <  SRR4302533.2853343/94‑1 (MQ=60)
           ATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAG                                                                                  >  SRR4302533.337850/1‑94 (MQ=60)
            TGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGG                                                                                 <  SRR4302533.1341049/94‑1 (MQ=60)
             GAACAACTTCTGCACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGG                                                                                <  SRR4302533.2777013/94‑1 (MQ=60)
             GAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGG                                                                                >  SRR4302533.3068278/1‑94 (MQ=60)
              AACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGA                                                                               <  SRR4302533.3317072/94‑1 (MQ=60)
              AACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGA                                                                               <  SRR4302533.3100786/94‑1 (MQ=60)
                CAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATG                                                                             <  SRR4302533.1425687/94‑1 (MQ=60)
                CAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATG                                                                             >  SRR4302533.5594498/1‑94 (MQ=60)
                CAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATG                                                                             >  SRR4302533.6168142/1‑94 (MQ=60)
                CAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATG                                                                             >  SRR4302533.1021028/1‑94 (MQ=60)
                  ACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCA                                                                           <  SRR4302533.892734/94‑1 (MQ=60)
                  ACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCA                                                                           <  SRR4302533.1945803/94‑1 (MQ=60)
                    TTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACCCCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGC                                                                         <  SRR4302533.4667285/94‑1 (MQ=60)
                    TTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGC                                                                         >  SRR4302533.5204795/1‑94 (MQ=60)
                    TTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGC                                                                         >  SRR4302533.1835076/1‑94 (MQ=60)
                     TCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCT                                                                        <  SRR4302533.5654887/94‑1 (MQ=60)
                     TCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCT                                                                        <  SRR4302533.5576053/94‑1 (MQ=60)
                     TCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCT                                                                        <  SRR4302533.4751437/94‑1 (MQ=60)
                     TCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCT                                                                        >  SRR4302533.1763815/1‑94 (MQ=60)
                      CTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGACAGCTTCCAGAAAGGGATGCAGCTT                                                                       >  SRR4302533.3248040/1‑94 (MQ=60)
                      CTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTT                                                                       >  SRR4302533.1985786/1‑94 (MQ=60)
                      CTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTT                                                                       <  SRR4302533.1274767/94‑1 (MQ=60)
                        ACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTA                                                                     <  SRR4302533.3844725/94‑1 (MQ=60)
                        ACACCCTGACCGTGTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTA                                                                     <  SRR4302533.870638/94‑1 (MQ=60)
                         CACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTAT                                                                    >  SRR4302533.313590/1‑94 (MQ=60)
                         CACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTAT                                                                    <  SRR4302533.5273145/94‑1 (MQ=60)
                            CCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTC                                                                 >  SRR4302533.4505592/1‑94 (MQ=60)
                             CTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCG                                                                >  SRR4302533.4480917/1‑94 (MQ=60)
                              TGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGA                                                               >  SRR4302533.3094497/1‑94 (MQ=60)
                              TGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTACCAGAAAGGGATGCAGCTTTATTTCGT                                                               >  SRR4302533.6347820/1‑94 (MQ=60)
                               GACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAG                                                              >  SRR4302533.5319700/1‑94 (MQ=60)
                               GACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAG                                                              >  SRR4302533.1053016/1‑94 (MQ=60)
                               GACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAG                                                              >  SRR4302533.1138687/1‑94 (MQ=60)
                               GACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAG                                                              <  SRR4302533.1312007/94‑1 (MQ=60)
                                ACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGC                                                             >  SRR4302533.653302/1‑94 (MQ=60)
                                ACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGC                                                             <  SRR4302533.6059832/94‑1 (MQ=60)
                                ACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGC                                                             <  SRR4302533.100314/94‑1 (MQ=60)
                                 CCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCG                                                            >  SRR4302533.5245918/1‑94 (MQ=60)
                                 CCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCG                                                            >  SRR4302533.3163364/1‑94 (MQ=60)
                                  CGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGT                                                           >  SRR4302533.2026382/1‑94 (MQ=60)
                                     TTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCAT                                                        <  SRR4302533.872414/94‑1 (MQ=60)
                                      TACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATG                                                       <  SRR4302533.1026939/94‑1 (MQ=60)
                                         GAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATG                                                    <  SRR4302533.4551496/94‑1 (MQ=60)
                                         GAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATG                                                    >  SRR4302533.1685669/1‑94 (MQ=60)
                                         GAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATG                                                    <  SRR4302533.5871779/94‑1 (MQ=60)
                                          AGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGG                                                   >  SRR4302533.4985108/1‑94 (MQ=60)
                                          AGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGG                                                   >  SRR4302533.182179/1‑94 (MQ=60)
                                            AAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTA                                                 <  SRR4302533.6082247/94‑1 (MQ=60)
                                            AAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTA                                                 <  SRR4302533.3520890/94‑1 (MQ=60)
                                            AAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTA                                                 >  SRR4302533.4331665/1‑94 (MQ=60)
                                             AGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAG                                                <  SRR4302533.1674149/94‑1 (MQ=60)
                                             AGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAG                                                >  SRR4302533.1666305/1‑94 (MQ=60)
                                             AGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAG                                                <  SRR4302533.522604/94‑1 (MQ=60)
                                                GCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGC                                             <  SRR4302533.297684/94‑1 (MQ=60)
                                                   CGGAAGTGATTCGCATGATCCACGCCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGC                                          <  SRR4302533.3567727/94‑1 (MQ=60)
                                                   CGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGC                                          >  SRR4302533.166681/1‑94 (MQ=60)
                                                      AAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGAC                                       >  SRR4302533.3224681/1‑94 (MQ=60)
                                                       AGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACC                                      <  SRR4302533.1148249/94‑1 (MQ=60)
                                                         TGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTG                                    >  SRR4302533.5613046/1‑94 (MQ=60)
                                                          GATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGT                                   >  SRR4302533.573073/1‑94 (MQ=60)
                                                            TTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGA                                 <  SRR4302533.4003320/94‑1 (MQ=60)
                                                            TTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGA                                 >  SRR4302533.2762181/1‑94 (MQ=60)
                                                             TCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGAC                                <  SRR4302533.3963163/94‑1 (MQ=60)
                                                             TCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGAC                                <  SRR4302533.3866764/94‑1 (MQ=60)
                                                             TCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGAC                                <  SRR4302533.329559/94‑1 (MQ=60)
                                                              CGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACG                               >  SRR4302533.6022529/1‑94 (MQ=60)
                                                              CGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACG                               <  SRR4302533.1681809/94‑1 (MQ=60)
                                                               GCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGCGACGA                              >  SRR4302533.4284889/1‑94 (MQ=60)
                                                                CATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGAC                             <  SRR4302533.5230318/94‑1 (MQ=60)
                                                                   GATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTT                          >  SRR4302533.2689519/1‑94 (MQ=60)
                                                                    ATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTG                         >  SRR4302533.3177398/1‑94 (MQ=60)
                                                                    ATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTG                         <  SRR4302533.5810640/94‑1 (MQ=60)
                                                                    ATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTG                         >  SRR4302533.733020/1‑94 (MQ=60)
                                                                     TCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGT                        >  SRR4302533.5237166/1‑94 (MQ=60)
                                                                     TCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGAAGTGCAGCGACCTGTGACGACTTTGT                        >  SRR4302533.6135834/1‑94 (MQ=60)
                                                                      CCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTG                       <  SRR4302533.1045918/94‑1 (MQ=60)
                                                                      CCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTG                       <  SRR4302533.2392974/94‑1 (MQ=60)
                                                                       CACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGC                      >  SRR4302533.4629121/1‑94 (MQ=60)
                                                                       CACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGC                      >  SRR4302533.638513/1‑94 (MQ=60)
                                                                        ACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCA                     >  SRR4302533.4069802/1‑94 (MQ=60)
                                                                        ACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCA                     >  SRR4302533.5903072/1‑94 (MQ=60)
                                                                         CACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAG                    >  SRR4302533.3481985/1‑94 (MQ=60)
                                                                         CACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAG                    <  SRR4302533.3051685/94‑1 (MQ=60)
                                                                          ACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGG                   <  SRR4302533.4887291/94‑1 (MQ=60)
                                                                           CCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGC                  <  SRR4302533.92022/94‑1 (MQ=60)
                                                                            CCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCG                 >  SRR4302533.11931/1‑94 (MQ=60)
                                                                            CCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCG                 <  SRR4302533.5404314/94‑1 (MQ=60)
                                                                             CTGCTTGGCGAAGCAAGCTTCTAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGA                >  SRR4302533.3130942/1‑94 (MQ=60)
                                                                             CTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGA                <  SRR4302533.452954/94‑1 (MQ=60)
                                                                              TGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGAT               <  SRR4302533.3577110/94‑1 (MQ=60)
                                                                              TGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGAT               <  SRR4302533.3444189/94‑1 (MQ=60)
                                                                              TGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGAT               >  SRR4302533.2510616/1‑94 (MQ=60)
                                                                                CTTGGCGAAGAAAGCTTCCGGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGAGCTGTGACGACTTTGTGCAGGCGAGGG             >  SRR4302533.1987789/1‑94 (MQ=60)
                                                                                CTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGG             >  SRR4302533.665017/1‑94 (MQ=60)
                                                                                  TGGCGAAGGAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAA           <  SRR4302533.5303271/94‑1 (MQ=60)
                                                                                  TGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAA           >  SRR4302533.3037487/1‑94 (MQ=60)
                                                                                   GGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAG          <  SRR4302533.2979106/94‑1 (MQ=60)
                                                                                     CGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGAT        <  SRR4302533.673600/94‑1 (MQ=60)
                                                                                     CGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGAT        >  SRR4302533.3896989/1‑94 (MQ=60)
                                                                                     CGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGAT        >  SRR4302533.2386303/1‑94 (MQ=60)
                                                                                     CGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGAT        >  SRR4302533.691577/1‑94 (MQ=60)
                                                                                      GAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATG       >  SRR4302533.2641992/1‑94 (MQ=60)
                                                                                      GAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATG       >  SRR4302533.2290573/1‑94 (MQ=60)
                                                                                      GAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATG       <  SRR4302533.1103211/94‑1 (MQ=60)
                                                                                      GAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATG       >  SRR4302533.5456964/1‑94 (MQ=60)
                                                                                        AGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCG     <  SRR4302533.3836763/94‑1 (MQ=60)
                                                                                        AGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCG     >  SRR4302533.4391998/1‑94 (MQ=60)
                                                                                        AGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCG     <  SRR4302533.3672262/94‑1 (MQ=60)
                                                                                        AGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCG     <  SRR4302533.1448416/94‑1 (MQ=60)
                                                                                         GAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGT    <  SRR4302533.3876893/94‑1 (MQ=60)
                                                                                         GAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGT    >  SRR4302533.326609/1‑94 (MQ=60)
                                                                                         GAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGT    <  SRR4302533.1004646/94‑1 (MQ=60)
                                                                                          AAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTC   <  SRR4302533.3091753/94‑1 (MQ=60)
                                                                                          AAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTC   >  SRR4302533.2026597/1‑94 (MQ=60)
                                                                                           AAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCG  <  SRR4302533.4872815/94‑1 (MQ=60)
                                                                                           AAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCG  <  SRR4302533.1744357/94‑1 (MQ=60)
                                                                                             |                                                                                           
TGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCG  >  NC_000913/990947‑991131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑