Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 991,040 A→G N399S (AAC→AGC)  pepN → aminopeptidase N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913991,0400AG99.2% 789.3 / ‑3.5 247N399S (AAC→AGC) pepNaminopeptidase N
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (109/136);  total (110/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

Only 100 of 256 total aligned reads displayed.

TCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGT  >  NC_000913/990950‑991129
                                                                                          |                                                                                         
tCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCt                                                                                         >  2:1639315/1‑93 (MQ=255)
tCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCt                                                                                         >  1:7588477/1‑93 (MQ=255)
 cATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCtt                                                                                        <  1:1455567/93‑1 (MQ=255)
  aTTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTc                                                                                       >  2:2400190/1‑93 (MQ=255)
  aTTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTc                                                                                       <  2:9511856/93‑1 (MQ=255)
   ttGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTcc                                                                                      <  2:6992711/93‑1 (MQ=255)
    tGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCa                                                                                     <  2:7240995/93‑1 (MQ=255)
     gagaTGAACAACTTCTACCCCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAg                                                                                    <  2:7853200/93‑1 (MQ=255)
     gagaTGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAg                                                                                    <  2:7743752/93‑1 (MQ=255)
      agaTGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGa                                                                                   <  2:9204529/93‑1 (MQ=255)
       gaTGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGaa                                                                                  >  1:7029356/1‑93 (MQ=255)
       gaTGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGaa                                                                                  <  1:6913782/93‑1 (MQ=255)
       gaTGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGaa                                                                                  <  1:4548984/93‑1 (MQ=255)
        aTGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCCTGCTCACCACCCTGCTTGGCGAAGAAAGCTTCCAGaag                                                                                 >  1:3619905/1‑92 (MQ=255)
         tGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAg                                                                                <  2:7428264/93‑1 (MQ=255)
          gAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAgg                                                                               <  2:9324793/93‑1 (MQ=255)
             caacTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGAt                                                                            <  2:5580032/93‑1 (MQ=255)
             caacTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGAt                                                                            >  1:5827083/1‑93 (MQ=255)
                cTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCa                                                                         >  1:3290416/1‑93 (MQ=255)
                 ttCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAg                                                                        >  1:8312495/1‑93 (MQ=255)
                  tCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGc                                                                       <  1:4134356/93‑1 (MQ=255)
                      cacCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTa                                                                   <  2:6957786/93‑1 (MQ=255)
                      cacCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTa                                                                   <  1:6609506/93‑1 (MQ=255)
                      cacCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTa                                                                   <  2:3809906/93‑1 (MQ=255)
                       acCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTAt                                                                  <  1:9077984/93‑1 (MQ=255)
                        cccTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTAtt                                                                 <  2:2835445/93‑1 (MQ=255)
                          cTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTc                                                               <  2:6215973/93‑1 (MQ=255)
                          cTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTc                                                               <  2:2537432/93‑1 (MQ=255)
                           tGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCg                                                              >  2:9347649/1‑93 (MQ=255)
                            gACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGa                                                             >  1:2167099/1‑93 (MQ=255)
                            gACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGACGCAGCTTTATTTCGa                                                             >  1:7177896/1‑93 (MQ=255)
                            gACCGTTTACGAGAAGGGCGCGGAAGTGATTCGAATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGa                                                             <  2:9524626/93‑1 (MQ=255)
                             aCCGTTTAGGAGAAGGGGGCGTGAGTGATTCGGATGAGCCACACTCTGCTTTGCGAGGAAAGCTGCCAGAAAGTGATGCAGCTTTATTTCGAg                                                            >  2:5776365/1‑93 (MQ=255)
                             aCCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAg                                                            <  2:8531699/93‑1 (MQ=255)
                               cGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCg                                                          >  2:5810193/1‑93 (MQ=255)
                                 tttACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTc                                                        <  2:6630540/93‑1 (MQ=255)
                                      gagaAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCatgat                                                   <  2:8119766/93‑1 (MQ=255)
                                       agaAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCatgatg                                                  >  2:7548898/1‑93 (MQ=255)
                                       agaAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCatgatg                                                  <  1:1806657/93‑1 (MQ=255)
                                       agaAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCatgatg                                                  >  2:5026832/1‑93 (MQ=255)
                                        gaAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATgg                                                 >  2:8353431/1‑93 (MQ=255)
                                         aaGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGt                                                <  1:7361288/93‑1 (MQ=255)
                                          aGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTa                                               >  2:6515775/1‑93 (MQ=255)
                                          aGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTa                                               <  2:2270451/93‑1 (MQ=255)
                                          aGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTa                                               <  1:1322860/93‑1 (MQ=255)
                                           gggCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAg                                              >  2:5139300/1‑93 (MQ=255)
                                           gggCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAg                                              <  1:5662121/93‑1 (MQ=255)
                                           gggCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCGTTATGTTGAGCGTCATGATGGTAg                                              >  1:8980118/1‑93 (MQ=255)
                                             gcgcGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTg                                            <  1:4400121/93‑1 (MQ=255)
                                              cgcgGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGc                                           >  2:4386004/1‑93 (MQ=255)
                                                cgGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAg                                         <  1:7075715/93‑1 (MQ=255)
                                                  gAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCg                                       >  2:4667086/1‑93 (MQ=255)
                                                  gAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCg                                       >  2:2755735/1‑93 (MQ=255)
                                                  gAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCg                                       >  2:9776788/1‑93 (MQ=255)
                                                  gAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCg                                       >  1:1594620/1‑93 (MQ=255)
                                                   aaGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGa                                      >  2:2992563/1‑93 (MQ=255)
                                                   aaGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGa                                      <  2:767452/93‑1 (MQ=255)
                                                     gTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGAcc                                    >  2:8832176/1‑93 (MQ=255)
                                                     gTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGAcc                                    <  2:3544421/93‑1 (MQ=255)
                                                     gTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGAcc                                    <  1:7675433/93‑1 (MQ=255)
                                                     gTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGAcc                                    <  1:7948253/93‑1 (MQ=255)
                                                     gTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGAcc                                    <  2:631339/93‑1 (MQ=255)
                                                       gATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCtg                                  <  2:6580590/93‑1 (MQ=255)
                                                        aTTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCtgt                                 <  1:8115499/93‑1 (MQ=255)
                                                        aTTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCtgt                                 <  1:9640683/93‑1 (MQ=255)
                                                          tCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCCACCtgtgc                               >  2:5338084/1‑92 (MQ=255)
                                                             cATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTgacga                            >  1:4228384/1‑93 (MQ=255)
                                                              aTGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTgacgac                           >  2:6455924/1‑93 (MQ=255)
                                                              aTGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTgacgac                           >  1:1910322/1‑93 (MQ=255)
                                                               tGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACt                          >  2:3726183/1‑93 (MQ=255)
                                                               tGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACt                          >  2:6010215/1‑93 (MQ=255)
                                                                  tCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGTTGGTAGTGCAGCGACCTGTGACGACTTtg                       <  2:2008011/93‑1 (MQ=255)
                                                                  tCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTtg                       >  1:9924170/1‑93 (MQ=255)
                                                                  tCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTtg                       <  2:3201940/93‑1 (MQ=255)
                                                                  tCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTtg                       >  2:1556168/1‑93 (MQ=255)
                                                                    cacaCCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTtgtg                     <  1:9318487/93‑1 (MQ=255)
                                                                      cacCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCa                   >  1:971879/1‑93 (MQ=255)
                                                                      cacCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCa                   >  2:3462913/1‑93 (MQ=255)
                                                                       acCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAg                  >  2:4548007/1‑93 (MQ=255)
                                                                       acCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAg                  >  2:4248290/1‑93 (MQ=255)
                                                                        cccTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAgg                 >  2:4522171/1‑93 (MQ=255)
                                                                        cccTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAgg                 >  1:4826714/1‑93 (MQ=255)
                                                                        cccTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAgg                 <  1:444484/93‑1 (MQ=255)
                                                                           tGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGa              >  2:1203225/1‑93 (MQ=255)
                                                                           tGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGa              <  1:9568798/93‑1 (MQ=255)
                                                                           tGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGa              <  1:4704301/93‑1 (MQ=255)
                                                                           tGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGa              >  2:2198631/1‑93 (MQ=255)
                                                                            gCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGAt             >  1:5458809/1‑93 (MQ=255)
                                                                             cTTGGCGAAGAAAGCTTCCAGAAAGGGATGCATCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATg            <  1:636006/93‑1 (MQ=255)
                                                                             cTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATg            <  2:3429928/93‑1 (MQ=255)
                                                                               tGGCGAAGAAATCTTCCAGAAAGGCATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGa          <  1:4037507/93‑1 (MQ=255)
                                                                                 gCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAg        >  1:2881527/1‑93 (MQ=255)
                                                                                  cGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGa       <  1:8743703/93‑1 (MQ=255)
                                                                                   gaagaaAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGAt      >  1:5885835/1‑93 (MQ=255)
                                                                                   gaagaaAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGAt      <  1:2671850/93‑1 (MQ=255)
                                                                                    aagaaAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATg     <  1:7138767/93‑1 (MQ=255)
                                                                                     agaaAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGc    <  1:6194991/93‑1 (MQ=255)
                                                                                     agaaAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGc    >  1:7113085/1‑93 (MQ=255)
                                                                                      gaaAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCg   <  2:8656285/93‑1 (MQ=255)
                                                                                      gaaAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCg   >  1:9256486/1‑93 (MQ=255)
                                                                                          |                                                                                         
TCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGT  >  NC_000913/990950‑991129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAA  >  NC_000913/990949‑991133
                                                                                           |                                                                                             
GTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCT                                                                                             >  SRR4302540.7673705/1‑94 (MQ=60)
  CATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTC                                                                                           <  SRR4302540.1471399/94‑1 (MQ=60)
  CATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTC                                                                                           <  SRR4302540.4560627/94‑1 (MQ=60)
    TTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCA                                                                                         <  SRR4302540.4605919/94‑1 (MQ=60)
    TTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCA                                                                                         <  SRR4302540.9867160/94‑1 (MQ=60)
     TGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAACGGCGCGGAAGTGATTCGCATGATCCCCACCCTGCTTGGCGAAGAAAGCCTCCAG                                                                                        >  SRR4302540.7125103/1‑94 (MQ=60)
      GAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGA                                                                                       <  SRR4302540.2537663/94‑1 (MQ=60)
       AGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAA                                                                                      >  SRR4302540.7107461/1‑94 (MQ=60)
        GATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCCTGCTCACCACCCTGCTTGGCGAAGAAAGCTTCCAGAAG                                                                                     >  SRR4302540.3659507/1‑94 (MQ=60)
        GATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAA                                                                                     <  SRR4302540.6990719/94‑1 (MQ=60)
        GATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAA                                                                                     <  SRR4302540.4598190/94‑1 (MQ=60)
        GATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAA                                                                                     <  SRR4302540.9288548/94‑1 (MQ=60)
        GATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAA                                                                                     >  SRR4302540.3659508/1‑94 (MQ=60)
         ATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAG                                                                                    <  SRR4302540.5015606/94‑1 (MQ=60)
         ATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACAACCTGCTTGGCGAAGAAAGCTTCCAGAAAG                                                                                    >  SRR4302540.4766151/1‑94 (MQ=60)
           GAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGG                                                                                  >  SRR4302540.4432682/1‑94 (MQ=60)
            AACAACTTCTACACCCTGACCGTTTACGAGCAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGA                                                                                 >  SRR4302540.4458380/1‑94 (MQ=60)
             ACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGAT                                                                                <  SRR4302540.4093537/94‑1 (MQ=60)
             ACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGAT                                                                                >  SRR4302540.5891503/1‑94 (MQ=60)
              CAACTTCTACCCCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATG                                                                               <  SRR4302540.8936005/94‑1 (MQ=60)
                ACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCA                                                                             >  SRR4302540.3326412/1‑94 (MQ=60)
                 CTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAG                                                                            >  SRR4302540.8408986/1‑94 (MQ=60)
                 CTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAG                                                                            >  SRR4302540.8408985/1‑94 (MQ=60)
                  TTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTTATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGC                                                                           >  SRR4302540.9494451/1‑94 (MQ=60)
                   TCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCT                                                                          <  SRR4302540.4179081/94‑1 (MQ=60)
                   TCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCT                                                                          <  SRR4302540.7183041/94‑1 (MQ=60)
                       CACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTAT                                                                      >  SRR4302540.2640710/1‑94 (MQ=60)
                       CACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTAT                                                                      >  SRR4302540.7242839/1‑94 (MQ=60)
                       CACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTAT                                                                      <  SRR4302540.6682693/94‑1 (MQ=60)
                        ACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATT                                                                     <  SRR4302540.9187030/94‑1 (MQ=60)
                        ACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATT                                                                     >  SRR4302540.7685340/1‑94 (MQ=60)
                          CCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTC                                                                   >  SRR4302540.6713971/1‑94 (MQ=60)
                            TGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGA                                                                 >  SRR4302540.2190455/1‑94 (MQ=60)
                            TGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGACGCAGCTTTATTTCGA                                                                 >  SRR4302540.7257911/1‑94 (MQ=60)
                               CCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCG                                                              <  SRR4302540.1482638/94‑1 (MQ=60)
                                CGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGT                                                             >  SRR4302540.7026621/1‑94 (MQ=60)
                                 GTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTC                                                            >  SRR4302540.4260134/1‑94 (MQ=60)
                                   TTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCTCCCCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATACAGCTTTATTTCGAGCGTCAT                                                          <  SRR4302540.4874988/94‑1 (MQ=60)
                                   TTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCAT                                                          <  SRR4302540.4284053/94‑1 (MQ=60)
                                   TTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCAT                                                          <  SRR4302540.933925/94‑1 (MQ=60)
                                       GAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATG                                                      >  SRR4302540.1706358/1‑94 (MQ=60)
                                       GAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATG                                                      >  SRR4302540.339984/1‑94 (MQ=60)
                                        AGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGG                                                     <  SRR4302540.1826341/94‑1 (MQ=60)
                                        AGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGG                                                     <  SRR4302540.1826340/94‑1 (MQ=60)
                                        AGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGG                                                     >  SRR4302540.3576823/1‑94 (MQ=60)
                                         GAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGT                                                    <  SRR4302540.9390174/94‑1 (MQ=60)
                                          AAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTA                                                   <  SRR4302540.7443372/94‑1 (MQ=60)
                                           AGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAG                                                  >  SRR4302540.6536440/1‑94 (MQ=60)
                                           AGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAG                                                  <  SRR4302540.9729604/94‑1 (MQ=60)
                                           AGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAG                                                  <  SRR4302540.1336649/94‑1 (MQ=60)
                                           AGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCGTTATGTTGAGCGTCATGATGGTAG                                                  >  SRR4302540.9087128/1‑94 (MQ=60)
                                            GGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGT                                                 <  SRR4302540.5724468/94‑1 (MQ=60)
                                              GCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGC                                               <  SRR4302540.4447844/94‑1 (MQ=60)
                                               CGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCA                                              <  SRR4302540.6647431/94‑1 (MQ=60)
                                                GCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAG                                             <  SRR4302540.6008258/94‑1 (MQ=60)
                                                GCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAG                                             <  SRR4302540.6008257/94‑1 (MQ=60)
                                                 CGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGC                                            <  SRR4302540.7154451/94‑1 (MQ=60)
                                                  GGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCG                                           >  SRR4302540.1612047/1‑94 (MQ=60)
                                                   GAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGA                                          >  SRR4302540.1570817/1‑94 (MQ=60)
                                                     AGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACC                                        >  SRR4302540.4834068/1‑94 (MQ=60)
                                                      GTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCT                                       <  SRR4302540.7762156/94‑1 (MQ=60)
                                                      GTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCT                                       >  SRR4302540.1366508/1‑94 (MQ=60)
                                                      GTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCT                                       <  SRR4302540.8040910/94‑1 (MQ=60)
                                                        GATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGT                                     <  SRR4302540.7594335/94‑1 (MQ=60)
                                                         ATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTG                                    <  SRR4302540.9754248/94‑1 (MQ=60)
                                                         ATTCGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTG                                    <  SRR4302540.8209685/94‑1 (MQ=60)
                                                            CGCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACG                                 >  SRR4302540.992804/1‑94 (MQ=60)
                                                             GCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGA                                <  SRR4302540.5939688/94‑1 (MQ=60)
                                                             GCATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGA                                >  SRR4302540.4274414/1‑94 (MQ=60)
                                                              CATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGAC                               >  SRR4302540.1931194/1‑94 (MQ=60)
                                                               ATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACT                              >  SRR4302540.4106547/1‑94 (MQ=60)
                                                               ATGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACT                              >  SRR4302540.7207504/1‑94 (MQ=60)
                                                                TGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTT                             >  SRR4302540.8176378/1‑94 (MQ=60)
                                                                TGATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTT                             <  SRR4302540.3183468/94‑1 (MQ=60)
                                                                 gagcgACACCCTGCCGGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGGGACGACTTT                            <  SRR4302540.7684201/89‑1 (MQ=60)
                                                                 GATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTT                            <  SRR4302540.7684200/94‑1 (MQ=60)
                                                                  ATCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTG                           >  SRR4302540.10040042/1‑94 (MQ=60)
                                                                   TCCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGT                          >  SRR4302540.5281595/1‑94 (MQ=60)
                                                                    CCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTG                         <  SRR4302540.5839192/94‑1 (MQ=60)
                                                                    CCACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATATCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTG                         <  SRR4302540.5839193/94‑1 (MQ=60)
                                                                     CACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGC                        <  SRR4302540.9429682/94‑1 (MQ=60)
                                                                      ACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCA                       >  SRR4302540.981875/1‑94 (MQ=60)
                                                                      ACACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCA                       >  SRR4302540.2827769/1‑94 (MQ=60)
                                                                       CACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAG                      <  SRR4302540.7788205/94‑1 (MQ=60)
                                                                       CACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAG                      <  SRR4302540.7887944/94‑1 (MQ=60)
                                                                        ACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGG                     <  SRR4302540.5708456/94‑1 (MQ=60)
                                                                        ACCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGG                     >  SRR4302540.4878401/1‑94 (MQ=60)
                                                                         CCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGC                    <  SRR4302540.449216/94‑1 (MQ=60)
                                                                         CCCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGC                    >  SRR4302540.6751409/1‑94 (MQ=60)
                                                                         CCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGC                    <  SRR4302540.3306796/94‑1 (MQ=60)
                                                                          CCTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCG                   <  SRR4302540.8306261/94‑1 (MQ=60)
                                                                           CTGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGA                  >  SRR4302540.2066428/1‑94 (MQ=60)
                                                                            TGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGAT                 <  SRR4302540.7348879/94‑1 (MQ=60)
                                                                            TGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGAT                 <  SRR4302540.9681863/94‑1 (MQ=60)
                                                                            TGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGAT                 <  SRR4302540.8895124/94‑1 (MQ=60)
                                                                            TGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGAT                 <  SRR4302540.4754573/94‑1 (MQ=60)
                                                                            TGCTTGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGAT                 >  SRR4302540.5518885/1‑94 (MQ=60)
                                                                              CTTGGCGAAGAAAGCTTCCAGAAAGGGATGCATCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGG               <  SRR4302540.642767/94‑1 (MQ=60)
                                                                                TGGCGAAGAAATCTTCCAGAAAGGCATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAA             <  SRR4302540.4081036/94‑1 (MQ=60)
                                                                                TGGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAA             <  SRR4302540.9795576/94‑1 (MQ=60)
                                                                                 GGCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAG            >  SRR4302540.2912880/1‑94 (MQ=60)
                                                                                  GCGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGA           <  SRR4302540.7153201/94‑1 (MQ=60)
                                                                                   CGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGAT          <  SRR4302540.960441/94‑1 (MQ=60)
                                                                                   CGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGAT          >  SRR4302540.5950862/1‑94 (MQ=60)
                                                                                   CGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGAT          <  SRR4302540.6359835/94‑1 (MQ=60)
                                                                                   CGAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGAT          <  SRR4302540.8845188/94‑1 (MQ=60)
                                                                                    GAAGAAAGCTTCCAGAAAGGGATTCAGCTTTATTTCTAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATG         >  SRR4302540.1862916/1‑94 (MQ=60)
                                                                                    GAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATG         >  SRR4302540.9692147/1‑94 (MQ=60)
                                                                                    GAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATG         <  SRR4302540.910559/94‑1 (MQ=60)
                                                                                    GAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATG         <  SRR4302540.2700789/94‑1 (MQ=60)
                                                                                    GAAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATG         <  SRR4302540.4554713/94‑1 (MQ=60)
                                                                                     AAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGC        >  SRR4302540.7192263/1‑94 (MQ=60)
                                                                                     AAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGC        <  SRR4302540.7218297/94‑1 (MQ=60)
                                                                                     AAGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGC        >  SRR4302540.8337899/1‑94 (MQ=60)
                                                                                      AGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCG       <  SRR4302540.7202433/94‑1 (MQ=60)
                                                                                      AGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCG       <  SRR4302540.6263964/94‑1 (MQ=60)
                                                                                      AGAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCG       >  SRR4302540.9367251/1‑94 (MQ=60)
                                                                                       GAAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGT      >  SRR4302540.7297221/1‑94 (MQ=60)
                                                                                        AAAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTC     >  SRR4302540.6440128/1‑94 (MQ=60)
                                                                                         AAGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCG    <  SRR4302540.1783825/94‑1 (MQ=60)
                                                                                          AGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGA   <  SRR4302540.6450947/94‑1 (MQ=60)
                                                                                          AGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGA   >  SRR4302540.8601611/1‑94 (MQ=60)
                                                                                          AGCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGA   >  SRR4302540.8601610/1‑94 (MQ=60)
                                                                                           GCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAA  >  SRR4302540.2581344/1‑94 (MQ=60)
                                                                                           GCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAA  >  SRR4302540.7458519/1‑94 (MQ=60)
                                                                                           GCTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAA  >  SRR4302540.1334221/1‑94 (MQ=60)
                                                                                           |                                                                                             
GTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAA  >  NC_000913/990949‑991133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑