Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,018,199	(A)_8→7	intergenic (+39/+37)	ycbG → / ← ompA	putative dehydrogenase/outer membrane protein 3a

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,018,192	0	A	.	96.1%	881.9 / 25.5	204	intergenic (+32/+44)	ycbG/ompA	putative dehydrogenase/outer membrane protein 3a
Reads supporting (aligned to +/- strand): ref base A (3/5); new base . (115/81); total (118/86)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.02e-01

Only 100 of 231 total aligned reads displayed.

ATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTC  >  NC_000913/1018100‑1018280

aTACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCa                                                                                          <  1:1041402/93‑1 (MQ=255)

 tACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCaa                                                                                         <  1:4080737/93‑1 (MQ=255)

  aCGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCaaa                                                                                        >  1:4671030/1‑93 (MQ=255)

     cgAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCaaaaaa                                                                                     >  2:2331150/1‑93 (MQ=255)

      gAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCaaaaaaa                                                                                    <  2:4959914/93‑1 (MQ=255)

      gAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCaaaaaaa                                                                                    <  1:5185361/93‑1 (MQ=255)

       aaTAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCaaaaaaac                                                                                   >  2:4626182/1‑92 (MQ=255)

       aaTAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCaaaaaaac                                                                                   <  1:2657804/93‑2 (MQ=255)

       aaTAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCaaaaaaac                                                                                   <  2:1454631/93‑2 (MQ=255)

       aaTAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCaaaaaaac                                                                                   <  1:1667399/93‑2 (MQ=255)

       aaTAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCaaaaaaac                                                                                   <  2:3465670/93‑2 (MQ=255)

       aaTAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCaaaaaaac                                                                                   >  1:5634162/1‑92 (MQ=255)

          aaaaTGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAcccc                                                                                <  2:4431460/93‑1 (MQ=255)

          aaaaTGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAcccc                                                                                <  2:2981525/93‑1 (MQ=255)

           aaaTGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAccccg                                                                               <  1:1812220/93‑2 (MQ=255)

             aTGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCgca                                                                            <  1:1626743/93‑1 (MQ=255)

             aTGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCgca                                                                            <  1:652734/93‑1 (MQ=255)

              tGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCgcag                                                                           >  1:3533110/1‑93 (MQ=255)

               gTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCgcagc                                                                          >  1:1007533/1‑93 (MQ=255)

               gTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCgcagc                                                                          <  2:330082/93‑1 (MQ=255)

                 cttctCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGcagcag                                                                        >  1:5001299/1‑93 (MQ=255)

                 cttctCTGAAGCAGGATCCGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGcagcag                                                                        <  1:1146244/93‑1 (MQ=255)

                  ttctCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCagcagc                                                                       <  2:3375190/93‑1 (MQ=255)

                  ttctCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCagcagc                                                                       <  1:3232926/93‑1 (MQ=255)

                  ttctCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCagcagc                                                                       >  2:3704287/1‑93 (MQ=255)

                   tctcTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCg                                                                      >  2:1153795/1‑93 (MQ=255)

                   tctcTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCg                                                                      >  2:762145/1‑93 (MQ=255)

                    tgctGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGGCAGTTCAATTTACTAAAGGC‑AAAAAATCCCCGCAGCAGCgg                                                                     <  2:2095491/91‑1 (MQ=255)

                     tctGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCggg                                                                    >  1:1428111/1‑93 (MQ=255)

                      ctGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCgggg                                                                   <  2:4913893/93‑1 (MQ=255)

                       tGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGt                                                                  >  1:1990216/1‑93 (MQ=255)

                         aaGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGttt                                                                <  2:6031006/93‑1 (MQ=255)

                          aGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGtttt                                                               >  2:1877950/1‑93 (MQ=255)

                          aGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGtttt                                                               >  2:5928534/1‑93 (MQ=255)

                           gCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGttttt                                                              >  2:1747939/1‑93 (MQ=255)

                            cAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTc                                                             <  1:1647075/93‑1 (MQ=255)

                             aGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAACCCCCGCAGCAGCGGGGTTTTTCt                                                            <  2:5808846/93‑1 (MQ=255)

                             aGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCt                                                            >  2:6303363/1‑93 (MQ=255)

                             aGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGATTTTCt                                                            >  2:2492258/1‑93 (MQ=255)

                              ggATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTa                                                           >  1:3807078/1‑93 (MQ=255)

                              ggATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTa                                                           >  1:2847216/1‑93 (MQ=255)

                                 tCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCa                                                        <  1:3644423/93‑1 (MQ=255)

                                    gCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGAc                                                     <  2:4742127/93‑1 (MQ=255)

                                    gCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGAc                                                     <  2:5111716/93‑1 (MQ=255)

                                    gCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGAc                                                     >  2:1032254/1‑93 (MQ=255)

                                    gCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGAc                                                     <  2:1248103/93‑1 (MQ=255)

                                    gCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGAc                                                     <  2:2256938/93‑1 (MQ=255)

                                      aGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACga                                                   <  2:4190546/93‑1 (MQ=255)

                                       ggCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACgag                                                  >  2:5579587/1‑93 (MQ=255)

                                       ggCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACgag                                                  <  1:5033903/93‑1 (MQ=255)

                                        gCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACgaga                                                 <  1:5720654/93‑1 (MQ=255)

                                           ttGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACt                                              >  2:3600570/1‑93 (MQ=255)

                                              cTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTaa                                           <  1:5886424/93‑1 (MQ=255)

                                               tGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAg                                          >  1:1542184/1‑93 (MQ=255)

                                                gggTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGc                                         >  1:350167/1‑93 (MQ=255)

                                                 ggTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGcc                                        >  1:2330329/1‑93 (MQ=255)

                                                   tAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAACCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTg                                      <  1:6206262/93‑1 (MQ=255)

                                                   tAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTg                                      <  1:4032079/93‑1 (MQ=255)

                                                   tAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTg                                      <  1:6010533/93‑1 (MQ=255)

                                                    aaGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGTTTTTTCTACCAGACGAGAACTTAAGCCTGc                                     <  1:6194662/93‑1 (MQ=255)

                                                     aGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCg                                    <  2:1578249/93‑1 (MQ=255)

                                                     aGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCg                                    >  1:3072613/1‑93 (MQ=255)

                                                     aGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCg                                    >  1:952502/1‑93 (MQ=255)

                                                      ggAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGGTTTTCTACCAGACGAGAACTTAAGCCTGCgg                                   >  1:83011/1‑93 (MQ=255)

                                                        aaTAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCt                                 >  2:1357359/1‑93 (MQ=255)

                                                          tAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGa                               <  2:5063134/93‑1 (MQ=255)

                                                          tAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGa                               >  2:4573040/1‑93 (MQ=255)

                                                          tAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGa                               <  2:5901350/93‑1 (MQ=255)

                                                            aCTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGt                             >  1:1936698/1‑93 (MQ=255)

                                                            aCTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGt                             <  1:4540593/93‑1 (MQ=255)

                                                             cTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGtt                            <  2:3440026/93‑1 (MQ=255)

                                                             cTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGtt                            <  1:1586033/93‑1 (MQ=255)

                                                              tGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTa                           >  1:2008681/1‑93 (MQ=255)

                                                              tGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTa                           >  1:2080103/1‑93 (MQ=255)

                                                               gACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTAc                          >  1:627101/1‑93 (MQ=255)

                                                               gACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTAc                          >  2:4042815/1‑93 (MQ=255)

                                                                aCGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACa                         >  1:1329679/1‑93 (MQ=255)

                                                                aCGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACa                         <  2:468035/93‑1 (MQ=255)

                                                                 cGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACaa                        >  2:2851283/1‑93 (MQ=255)

                                                                 cGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACaa                        >  2:2007162/1‑93 (MQ=255)

                                                                 cGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACaa                        <  2:3058544/93‑1 (MQ=255)

                                                                    aaGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGt                     <  2:3889442/93‑1 (MQ=255)

                                                                     aGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTc                    >  2:6205665/1‑93 (MQ=255)

                                                                      gTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCt                   <  1:5093821/93‑1 (MQ=255)

                                                                      gTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCt                   >  2:1776670/1‑93 (MQ=255)

                                                                        cAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCttt                 <  2:5283774/93‑1 (MQ=255)

                                                                         aGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTg                <  1:2427850/93‑1 (MQ=255)

                                                                          gTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGa               >  2:5504141/1‑93 (MQ=255)

                                                                          gTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGa               <  2:1960988/93‑1 (MQ=255)

                                                                            tCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATa             <  2:52427/93‑1 (MQ=255)

                                                                              aaTTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATAcc           <  2:1809096/93‑1 (MQ=255)

                                                                              aaTTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATAcc           >  1:1857985/1‑93 (MQ=255)

                                                                                tttACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCtt         >  2:2181908/1‑93 (MQ=255)

                                                                                 ttACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCttt        <  2:2379256/93‑1 (MQ=255)

                                                                                 ttACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCttt        >  1:4444668/1‑93 (MQ=255)

                                                                                   aCTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTaa      <  1:3999517/93‑1 (MQ=255)

                                                                                   aCTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCGTTaa      >  1:2055770/1‑93 (MQ=255)

                                                                                     tAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACt    >  1:5349743/1‑93 (MQ=255)

                                                                                     tAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACt    >  2:2765827/1‑93 (MQ=255)

                                                                                       aaGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTc  >  1:4523025/1‑93 (MQ=255)

ATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTC  >  NC_000913/1018100‑1018280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCG  >  NC_000913/1018106‑1018292

GAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAC                                                                                               <  SRR4302533.5216660/94‑1 (MQ=60)

GAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAC                                                                                               >  SRR4302533.5668231/1‑94 (MQ=60)

 AATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAACC                                                                                              <  SRR4302533.2674133/94‑1 (MQ=60)

 AATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAACC                                                                                              <  SRR4302533.1677472/94‑1 (MQ=60)

 AATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAACC                                                                                              >  SRR4302533.5896980/1‑94 (MQ=60)

   TAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAACCCC                                                                                            <  SRR4302533.4539095/94‑1 (MQ=60)

     AAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGC                                                                                         <  SRR4302533.1823290/94‑1 (MQ=60)

      AATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCA                                                                                        >  SRR4302533.6312224/1‑94 (MQ=60)

       ATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAG                                                                                       >  SRR4302533.3554882/1‑94 (MQ=60)

       ATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAG                                                                                       <  SRR4302533.3713435/94‑1 (MQ=60)

       ATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAG                                                                                       <  SRR4302533.656587/94‑1 (MQ=60)

       ATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAG                                                                                       >  SRR4302533.6150918/1‑94 (MQ=60)

       ATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAG                                                                                       <  SRR4302533.1636594/94‑1 (MQ=60)

        TGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGC                                                                                      >  SRR4302533.1013427/1‑94 (MQ=60)

          TCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAG                                                                                    >  SRR4302533.5031616/1‑94 (MQ=60)

          TCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAG                                                                                    >  SRR4302533.3123669/1‑94 (MQ=60)

           CTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGC                                                                                   >  SRR4302533.4409304/1‑94 (MQ=60)

           CTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGC                                                                                   <  SRR4302533.1847343/94‑1 (MQ=60)

           CTTCTCTGAAGCAGGATCCGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGC                                                                                   <  SRR4302533.1153084/94‑1 (MQ=60)

            TTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCG                                                                                  >  SRR4302533.6017790/1‑94 (MQ=60)

            TTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCG                                                                                  <  SRR4302533.3252847/94‑1 (MQ=60)

             TCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGG                                                                                 >  SRR4302533.2248300/1‑94 (MQ=60)

              CTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGG                                                                                >  SRR4302533.1436744/1‑94 (MQ=60)

              CTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGG                                                                                >  SRR4302533.3294024/1‑94 (MQ=60)

               TCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGG                                                                               >  SRR4302533.4149818/1‑94 (MQ=60)

                CTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGT                                                                              >  SRR4302533.2002404/1‑94 (MQ=60)

                    AGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTT                                                                          >  SRR4302533.2004041/1‑94 (MQ=60)

                     GCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTC                                                                         <  SRR4302533.2071028/94‑1 (MQ=60)

                      CAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCT                                                                        <  SRR4302533.1657049/94‑1 (MQ=60)

                       AGGATCTGCAGGCATTGCTGTGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTA                                                                       >  SRR4302533.1518407/1‑94 (MQ=60)

                       AGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGGAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTA                                                                       >  SRR4302533.1984212/1‑94 (MQ=60)

                       AGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTA                                                                       >  SRR4302533.2864738/1‑94 (MQ=60)

                       AGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTA                                                                       >  SRR4302533.3830370/1‑94 (MQ=60)

                         GATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACC                                                                     >  SRR4302533.2698918/1‑94 (MQ=60)

                           TCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAG                                                                   <  SRR4302533.938432/94‑1 (MQ=60)

                           TCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAG                                                                   <  SRR4302533.3666830/94‑1 (MQ=60)

                             TGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGAC                                                                 <  SRR4302533.793708/94‑1 (MQ=60)

                              GCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACG                                                                <  SRR4302533.970156/94‑1 (MQ=60)

                              GCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACG                                                                >  SRR4302533.6237767/1‑94 (MQ=60)

                              ACAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTACAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACG                                                                <  SRR4302533.6334024/94‑1 (MQ=60)

                               CAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGA                                                               >  SRR4302533.1719671/1‑94 (MQ=60)

                                AGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAG                                                              >  SRR4302533.2358852/1‑94 (MQ=60)

                                AGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAG                                                              <  SRR4302533.4558824/94‑1 (MQ=60)

                                 GGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGA                                                             <  SRR4302533.5064396/94‑1 (MQ=60)

                                  GCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAA                                                            <  SRR4302533.5755194/94‑1 (MQ=60)

                                    ATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACT                                                          >  SRR4302533.1629599/1‑94 (MQ=60)

                                        CTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAG                                                      >  SRR4302533.1551474/1‑94 (MQ=60)

                                        CTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAG                                                      <  SRR4302533.5921990/94‑1 (MQ=60)

                                        CTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAG                                                      >  SRR4302533.5175713/1‑94 (MQ=60)

                                         TGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGC                                                     <  SRR4302533.1747824/94‑1 (MQ=60)

                                         TGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGC                                                     >  SRR4302533.352301/1‑94 (MQ=60)

                                          GGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCC                                                    <  SRR4302533.1001217/94‑1 (MQ=60)

                                          GGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCC                                                    >  SRR4302533.2344758/1‑94 (MQ=60)

                                            GTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTG                                                  >  SRR4302533.5593429/1‑94 (MQ=60)

                                            GTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTG                                                  >  SRR4302533.3789222/1‑94 (MQ=60)

                                             TAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAACCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGC                                                 <  SRR4302533.6243482/94‑1 (MQ=60)

                                             TAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGC                                                 <  SRR4302533.6046948/94‑1 (MQ=60)

                                             TAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGC                                                 <  SRR4302533.4056625/94‑1 (MQ=60)

                                              AAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGTTTTTTCTACCAGACGAGAACTTAAGCCTGCG                                                <  SRR4302533.6231831/94‑1 (MQ=60)

                                              AAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCG                                                >  SRR4302533.958097/1‑94 (MQ=60)

                                              AAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCG                                                >  SRR4302533.3091475/1‑94 (MQ=60)

                                               AGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGG                                               >  SRR4302533.1075985/1‑94 (MQ=60)

                                               AGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGGTTTTCTACCAGACGAGAACTTAAGCCTGCGG                                               >  SRR4302533.83538/1‑94 (MQ=60)

                                                 GAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCT                                             >  SRR4302533.4981879/1‑94 (MQ=60)

                                                   ATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGA                                           >  SRR4302533.1942869/1‑94 (MQ=60)

                                                    TAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAG                                          <  SRR4302533.5034421/94‑1 (MQ=60)

                                                    TAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAG                                          <  SRR4302533.257176/94‑1 (MQ=60)

                                                     AACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGT                                         >  SRR4302533.1948509/1‑94 (MQ=60)

                                                     AACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGT                                         >  SRR4302533.5073996/1‑94 (MQ=60)

                                                      ACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTT                                        <  SRR4302533.4568085/94‑1 (MQ=60)

                                                      ACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTT                                        >  SRR4302533.6267047/1‑94 (MQ=60)

                                                       CTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTA                                       >  SRR4302533.2092900/1‑94 (MQ=60)

                                                       CTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTA                                       <  SRR4302533.1595589/94‑1 (MQ=60)

                                                       CTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTA                                       >  SRR4302533.2021051/1‑94 (MQ=60)

                                                        TGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTAC                                      >  SRR4302533.630787/1‑94 (MQ=60)

                                                         GACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACA                                     >  SRR4302533.1337657/1‑94 (MQ=60)

                                                           CGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAAC                                   <  SRR4302533.2119073/94‑1 (MQ=60)

                                                            GAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACG                                  <  SRR4302533.1981529/94‑1 (MQ=60)

                                                            GAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACG                                  >  SRR4302533.1044671/1‑94 (MQ=60)

                                                              AAGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTC                                >  SRR4302533.1413402/1‑94 (MQ=60)

                                                               AGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCT                               >  SRR4302533.469622/1‑94 (MQ=60)

                                                               AGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCT                               <  SRR4302533.3527460/94‑1 (MQ=60)

                                                               AGTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCT                               <  SRR4302533.1642406/94‑1 (MQ=60)

                                                                GTCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTT                              <  SRR4302533.5124592/94‑1 (MQ=60)

                                                                 TCAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTT                             <  SRR4302533.4059089/94‑1 (MQ=60)

                                                                  CAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTG                            >  SRR4302533.5754221/1‑94 (MQ=60)

                                                                  CAGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTG                            <  SRR4302533.5110764/94‑1 (MQ=60)

                                                                   AGTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGA                           <  SRR4302533.2442859/94‑1 (MQ=60)

                                                                    GTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGAT                          >  SRR4302533.1671717/1‑94 (MQ=60)

                                                                    GTTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGAT                          <  SRR4302533.2039223/94‑1 (MQ=60)

                                                                     TTCAATTTACTAAATGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATA                         <  SRR4302533.5361770/94‑1 (MQ=60)

                                                                     TTCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATA                         <  SRR4302533.3367441/94‑1 (MQ=60)

                                                                      TCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATAC                        >  SRR4302533.1435236/1‑94 (MQ=60)

                                                                      TCAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATAC                        >  SRR4302533.5441016/1‑94 (MQ=60)

                                                                       CAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACC                       <  SRR4302533.3575422/94‑1 (MQ=60)

                                                                       CAATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACC                       >  SRR4302533.1869316/1‑94 (MQ=60)

                                                                         ATTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGTACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTT                     <  SRR4302533.4582214/94‑1 (MQ=60)

                                                                          TTTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTT                    >  SRR4302533.4471551/1‑94 (MQ=60)

                                                                           TTACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTA                   >  SRR4302533.2891184/1‑94 (MQ=60)

                                                                            TACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCGTTAA                  >  SRR4302533.2068451/1‑94 (MQ=60)

                                                                             ACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAAC                 <  SRR4302533.4023869/94‑1 (MQ=60)

                                                                             ACTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAAC                 <  SRR4302533.2690667/94‑1 (MQ=60)

                                                                              CTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACT                <  SRR4302533.1586425/94‑1 (MQ=60)

                                                                              CTAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACT                >  SRR4302533.5381842/1‑94 (MQ=60)

                                                                               TAAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTT               <  SRR4302533.4049466/94‑1 (MQ=60)

                                                                                AAAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTC              >  SRR4302533.4550405/1‑94 (MQ=60)

                                                                                 AAGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCG             >  SRR4302533.5869408/1‑94 (MQ=60)

                                                                                  acggcAAAAAACCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCG             <  SRR4302533.6336494/89‑1 (MQ=60)

                                                                                  AGGC‑AAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGGGGGTGAGTTACAACGTCTTTGATACCTTTAACTTCGA            <  SRR4302533.2404143/94‑1 (MQ=60)

                                                                                   GGC‑AAAAAACCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGAT           <  SRR4302533.4943292/94‑1 (MQ=60)

                                                                                     gcAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATC          <  SRR4302533.1222253/92‑1 (MQ=60)

                                                                                        AAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCT       >  SRR4302533.4960614/1‑94 (MQ=60)

                                                                                         AAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTA      >  SRR4302533.4661135/1‑94 (MQ=60)

                                                                                         AAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTA      >  SRR4302533.1882662/1‑94 (MQ=60)

                                                                                          AAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTAC     <  SRR4302533.2584203/94‑1 (MQ=60)

                                                                                          AAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTAC     <  SRR4302533.5018685/94‑1 (MQ=60)

                                                                                          AAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTAC     <  SRR4302533.3297271/94‑1 (MQ=60)

                                                                                           AACCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACG    <  SRR4302533.770984/94‑1 (MQ=60)

                                                                                           AAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACG    >  SRR4302533.4439462/1‑94 (MQ=60)

                                                                                           AAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACG    >  SRR4302533.3668098/1‑94 (MQ=60)

                                                                                             ACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCG  >  SRR4302533.3305996/1‑94 (MQ=60)

GAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCG  >  NC_000913/1018106‑1018292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`