Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,029,120	+C	coding (73/1191 nt)	b0967 ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,029,116	1	.	C	99.1%	664.3 / ‑2.1	211	coding (77/1191 nt)	b0967	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (100/109); total (102/109)
Fisher's exact test for biased strand distribution p-value = 2.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

GCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGG‑CCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTT  >  NC_000913/1029024‑1029203

  gAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGG‑cc                                                                                       >  1:318945/1‑93 (MQ=255)

   aaTAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGG‑ccc                                                                                      >  2:800544/1‑93 (MQ=255)

   aaTAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGG‑ccc                                                                                      >  1:786482/1‑93 (MQ=255)

    aTAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGG‑cccc                                                                                     <  2:5983550/93‑1 (MQ=255)

    aTAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGG‑cccc                                                                                     >  1:1636117/1‑93 (MQ=255)

        gcgcCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGG‑cccccgga                                                                                 >  2:3374604/1‑89 (MQ=255)

           ccgcGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCGATGCGGGCAACGGCCCCCGGaaaa                                                                               <  1:3847324/93‑1 (MQ=255)

             gcgTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGa                                                                             >  2:2226840/1‑93 (MQ=255)

             gcgTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGa                                                                             <  1:6203001/93‑1 (MQ=255)

             gcgTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGa                                                                             >  1:5103028/1‑93 (MQ=255)

               gTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGAcc                                                                           >  2:2431283/1‑93 (MQ=255)

               gTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGAcc                                                                           >  1:172588/1‑93 (MQ=255)

                 gCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCa                                                                         <  2:630025/93‑1 (MQ=255)

                 gCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCa                                                                         >  2:3790194/1‑93 (MQ=255)

                  cTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCAc                                                                        >  1:66638/1‑93 (MQ=255)

                   tAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACg                                                                       <  2:1201665/93‑1 (MQ=255)

                   tAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACg                                                                       >  1:2071/1‑93 (MQ=255)

                     aCCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGa                                                                     <  2:4343229/93‑1 (MQ=255)

                      ccATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGAt                                                                    <  1:3422529/93‑1 (MQ=255)

                      ccATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGAt                                                                    <  2:4579592/93‑1 (MQ=255)

                      ccAGTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGAt                                                                    <  1:5692944/93‑1 (MQ=255)

                       cATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATg                                                                   <  2:3138160/93‑1 (MQ=255)

                        aTTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATgg                                                                  <  1:511211/93‑1 (MQ=255)

                         ttttCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGc                                                                 <  2:1993407/93‑1 (MQ=255)

                            tCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGcgac                                                              >  2:2848950/1‑93 (MQ=255)

                             cccTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGcgacg                                                             >  1:3240335/1‑93 (MQ=255)

                               cTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGaa                                                           >  1:1041111/1‑93 (MQ=255)

                               cTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGaa                                                           <  2:1842246/93‑1 (MQ=255)

                               cTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGaa                                                           >  1:4382171/1‑93 (MQ=255)

                                 gatgatCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAga                                                         >  2:5596102/1‑92 (MQ=255)

                                    gatCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAAt                                                      <  1:2713255/93‑1 (MQ=255)

                                     atCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATg                                                     <  2:675351/93‑1 (MQ=255)

                                     atCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATg                                                     <  1:4729494/93‑1 (MQ=255)

                                     atCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATg                                                     <  1:6165278/93‑1 (MQ=255)

                                      tCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGa                                                    >  1:3888736/1‑93 (MQ=255)

                                      tCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGa                                                    >  2:5234443/1‑93 (MQ=255)

                                      tCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGa                                                    >  1:6293436/1‑93 (MQ=255)

                                      tCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGa                                                    >  1:2813910/1‑93 (MQ=255)

                                       caacaaTATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGAt                                                   <  1:5802536/93‑1 (MQ=255)

                                        aacaaTATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGAtt                                                  >  1:6080089/1‑93 (MQ=255)

                                        aacaaTATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGAtt                                                  >  1:4883789/1‑93 (MQ=255)

                                         acaaTATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGAttt                                                 <  2:3542193/93‑1 (MQ=255)

                                          caaTATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGAtttt                                                >  2:5729001/1‑93 (MQ=255)

                                          caaTATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGAtttt                                                <  2:4294524/93‑1 (MQ=255)

                                          caaTATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGAtttt                                                <  1:1698074/93‑1 (MQ=255)

                                           aaTATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGGAATGAttttt                                               >  2:422503/1‑93 (MQ=255)

                                            atatCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTc                                              <  1:5070985/93‑1 (MQ=255)

                                            atatCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTc                                              <  2:288910/93‑1 (MQ=255)

                                              atCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTcgc                                            >  2:5764402/1‑93 (MQ=255)

                                               tCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTcgcg                                           >  1:1529900/1‑93 (MQ=255)

                                               tCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTcgcg                                           >  1:600345/1‑93 (MQ=255)

                                                cGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCgcgc                                          >  1:5586308/1‑93 (MQ=255)

                                                 gATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGcc                                         >  2:300884/1‑93 (MQ=255)

                                                 gATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGcc                                         >  2:6076907/1‑93 (MQ=255)

                                                  aTGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGccc                                        >  2:6276662/1‑93 (MQ=255)

                                                    ggTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCtt                                      <  1:1001738/93‑1 (MQ=255)

                                                    ggTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCtt                                      <  2:1604901/93‑1 (MQ=255)

                                                     gTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCttt                                     >  1:1484543/1‑93 (MQ=255)

                                                      tttCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTATTGATTTTTCGCGCCCTTTg                                    <  1:2544537/93‑1 (MQ=255)

                                                      tttCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTg                                    <  2:4687422/93‑1 (MQ=255)

                                                       ttCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTgg                                   <  2:1756364/93‑1 (MQ=255)

                                                       ttCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTgg                                   <  1:2298887/93‑1 (MQ=255)

                                                        tCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACTCACGGATGGCGACGAAGCAATGATTATTTGCGCCATTTTGc                                  >  1:3004241/1‑93 (MQ=255)

                                                        tCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGc                                  <  2:2058367/93‑1 (MQ=255)

                                                        tCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGc                                  <  2:4467946/93‑1 (MQ=255)

                                                         cACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCt                                 >  2:482004/1‑93 (MQ=255)

                                                            cGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAac                              <  1:6223756/93‑1 (MQ=255)

                                                             gAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAaca                             >  1:2230633/1‑93 (MQ=255)

                                                             gAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAaca                             <  1:242495/93‑1 (MQ=255)

                                                             gAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAaca                             <  2:3046310/93‑1 (MQ=255)

                                                              aGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAacac                            >  1:1380169/1‑93 (MQ=255)

                                                                gctggctTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTa                          <  2:3959805/93‑1 (MQ=255)

                                                                  tggctTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTaaa                        <  1:339127/93‑1 (MQ=255)

                                                                  tggctTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTaaa                        >  1:2146920/1‑93 (MQ=255)

                                                                  tggctTTACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTaaa                        <  2:4746934/93‑1 (MQ=255)

                                                                      tttACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTa                    <  2:3880496/93‑1 (MQ=255)

                                                                       ttACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTac                   <  1:1828395/93‑1 (MQ=255)

                                                                       ttACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTac                   <  2:1088130/93‑1 (MQ=255)

                                                                       ttACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTac                   >  1:1258699/1‑93 (MQ=255)

                                                                        tACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTaca                  <  1:2878306/93‑1 (MQ=255)

                                                                        tACCTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTaca                  <  1:1318260/93‑1 (MQ=255)

                                                                         tccTTCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTacac                 <  2:4848171/92‑1 (MQ=255)

                                                                            ttCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCa              >  1:2392810/1‑93 (MQ=255)

                                                                            ttCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCa              <  2:5090721/93‑1 (MQ=255)

                                                                            ttCCATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCa              <  2:2513800/93‑1 (MQ=255)

                                                                              ccATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATa            <  1:2918947/93‑1 (MQ=255)

                                                                              ccATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATa            <  2:1115677/93‑1 (MQ=255)

                                                                               cATGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATaa           <  1:3723776/93‑1 (MQ=255)

                                                                                aTGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAAt          >  1:1340955/1‑93 (MQ=255)

                                                                                aTGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAAt          <  2:2816337/93‑1 (MQ=255)

                                                                                aTGCGGCCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAAt          <  2:2244307/93‑1 (MQ=255)

                                                                                 tGCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAAtt         <  2:2657925/93‑1 (MQ=255)

                                                                                  gCGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAAttt        <  2:1486622/93‑1 (MQ=255)

                                                                                   cGGGCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTa       <  2:2269453/93‑1 (MQ=255)

                                                                                     ggCAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACt     >  1:4769262/1‑93 (MQ=255)

                                                                                       cAACGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTCCttt   <  1:4068490/93‑1 (MQ=255)

                                                                                        aaCGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACtttt  <  2:5212012/93‑1 (MQ=255)

                                                                                        aaCGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACtttt  >  1:4276790/1‑93 (MQ=255)

                                                                                        aaCGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACtttt  <  1:5550637/93‑1 (MQ=255)

                                                                                        aaCGGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACtttt  <  2:3286014/93‑1 (MQ=255)

GCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGG‑CCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTT  >  NC_000913/1029024‑1029203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GG‑CCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATG  >  NC_000913/1029027‑1029212

AATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GG‑CCCC                                                                                              >  SRR4302533.1646024/1‑94 (MQ=60)

 ATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GG‑CCCCC                                                                                             >  SRR4302533.321399/1‑94 (MQ=60)

   AAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GG‑CCCCcgg                                                                                           >  SRR4302533.99013/1‑91 (MQ=60)

   AAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GG‑CCCCcgg                                                                                           <  SRR4302533.580025/94‑4 (MQ=60)

        CCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCGATGCGGGCAAC‑GGCCCCCGGAAAAG                                                                                       <  SRR4302533.3870817/94‑1 (MQ=60)

         CGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAACAGA                                                                                      >  SRR4302533.6321411/1‑94 (MQ=60)

         CGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGA                                                                                      >  SRR4302533.5133855/1‑94 (MQ=60)

          GCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGAC                                                                                     <  SRR4302533.6240203/94‑1 (MQ=60)

           CGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACC                                                                                    >  SRR4302533.2347939/1‑94 (MQ=60)

           CGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACC                                                                                    >  SRR4302533.173677/1‑94 (MQ=60)

            GTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCC                                                                                   >  SRR4302533.342314/1‑94 (MQ=60)

              TCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCAC                                                                                 <  SRR4302533.6252457/94‑1 (MQ=60)

              GCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCAC                                                                                 >  SRR4302533.67071/1‑94 (MQ=60)

               CTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACG                                                                                >  SRR4302533.1523361/1‑94 (MQ=60)

               CTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACG                                                                                >  SRR4302533.2086/1‑94 (MQ=60)

               CTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACG                                                                                >  SRR4302533.6193694/1‑94 (MQ=60)

                TAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGG                                                                               >  SRR4302533.2125582/1‑94 (MQ=60)

                TAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGG                                                                               <  SRR4302533.3902921/94‑1 (MQ=60)

                TAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGG                                                                               >  SRR4302533.2520100/1‑94 (MQ=60)

                   acagTTTCCCTGATGATCAACAGTATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATG                                                                            <  SRR4302533.3346151/90‑1 (MQ=60)

                   CCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATG                                                                            <  SRR4302533.3443672/94‑1 (MQ=60)

                   CCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATG                                                                            <  SRR4302533.6337094/94‑1 (MQ=60)

                   CCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATG                                                                            <  SRR4302533.1326338/94‑1 (MQ=60)

                   CCATTTTCCCTGATGATCAACAATATCGATGGATTCACCGAGGCTGGCTTTACATTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATG                                                                            >  SRR4302533.2161020/1‑94 (MQ=60)

                   CCAGTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATG                                                                            <  SRR4302533.5727324/94‑1 (MQ=60)

                    CATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGG                                                                           >  SRR4302533.4086045/1‑94 (MQ=60)

                    CATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGG                                                                           >  SRR4302533.2268944/1‑94 (MQ=60)

                     ATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGC                                                                          <  SRR4302533.514249/94‑1 (MQ=60)

                     ATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGC                                                                          >  SRR4302533.1697053/1‑94 (MQ=60)

                      TTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCG                                                                         <  SRR4302533.17347/94‑1 (MQ=60)

                        TTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGAC                                                                       <  SRR4302533.261965/94‑1 (MQ=60)

                        TTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGAC                                                                       <  SRR4302533.4483490/94‑1 (MQ=60)

                         TCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACG                                                                      >  SRR4302533.3260301/1‑94 (MQ=60)

                          CCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGA                                                                     >  SRR4302533.5580366/1‑94 (MQ=60)

                           CCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAA                                                                    >  SRR4302533.4408629/1‑94 (MQ=60)

                           CCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAA                                                                    >  SRR4302533.1047231/1‑94 (MQ=60)

                               ATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAA                                                                >  SRR4302533.511410/1‑94 (MQ=60)

                               ATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAA                                                                >  SRR4302533.6057802/1‑94 (MQ=60)

                               ATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAA                                                                >  SRR4302533.675936/1‑94 (MQ=60)

                                TGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAAT                                                               >  SRR4302533.2421755/1‑94 (MQ=60)

                                 GATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATG                                                              <  SRR4302533.2729917/94‑1 (MQ=60)

                                 GATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATG                                                              <  SRR4302533.1288994/94‑1 (MQ=60)

                                  ATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGA                                                             <  SRR4302533.4758166/94‑1 (MQ=60)

                                  ATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGA                                                             <  SRR4302533.1599327/94‑1 (MQ=60)

                                  ATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGA                                                             >  SRR4302533.6330954/1‑94 (MQ=60)

                                  ATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGA                                                             >  SRR4302533.6153495/1‑94 (MQ=60)

                                  ATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGA                                                             >  SRR4302533.3912461/1‑94 (MQ=60)

                                  ATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGA                                                             >  SRR4302533.2831229/1‑94 (MQ=60)

                                  ATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGA                                                             <  SRR4302533.6202339/94‑1 (MQ=60)

                                  ATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGGAATGA                                                             >  SRR4302533.2831071/1‑94 (MQ=60)

                                    CAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAGAGACCCACGGATGGCGACGAAGTAATGATT                                                           >  SRR4302533.1365731/1‑94 (MQ=60)

                                    CAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATT                                                           <  SRR4302533.5837586/94‑1 (MQ=60)

                                    CAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATT                                                           >  SRR4302533.6116859/1‑94 (MQ=60)

                                    CAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATT                                                           >  SRR4302533.3849173/1‑94 (MQ=60)

                                    CAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATT                                                           >  SRR4302533.4913378/1‑94 (MQ=60)

                                     AACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGAGACGAAGTAATGATTT                                                          >  SRR4302533.5355665/1‑94 (MQ=60)

                                       CAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTT                                                        <  SRR4302533.1708364/94‑1 (MQ=60)

                                         ATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCG                                                      <  SRR4302533.5101638/94‑1 (MQ=60)

                                         ATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCG                                                      <  SRR4302533.5975115/94‑1 (MQ=60)

                                           ATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGG‑CCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCG                                                    >  SRR4302533.4089026/1‑94 (MQ=60)

                                           ATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCG                                                    >  SRR4302533.1539098/1‑94 (MQ=60)

                                           ATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCG                                                    >  SRR4302533.603875/1‑94 (MQ=60)

                                            TCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGC                                                   >  SRR4302533.5620062/1‑94 (MQ=60)

                                             CGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCC                                                  >  SRR4302533.3536561/1‑94 (MQ=60)

                                              GATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGCCGAAGTAATGATTTTTCGCGCCC                                                 >  SRR4302533.5777616/1‑94 (MQ=60)

                                               ATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCT                                                >  SRR4302533.1430468/1‑94 (MQ=60)

                                                TGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTT                                               <  SRR4302533.4264668/94‑1 (MQ=60)

                                                TGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGCAATGATTTTTCGTGCCCTT                                               >  SRR4302533.3585657/1‑94 (MQ=60)

                                                 GGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTT                                              <  SRR4302533.1007588/94‑1 (MQ=60)

                                                 GGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTT                                              >  SRR4302533.1493502/1‑94 (MQ=60)

                                                  GTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTG                                             <  SRR4302533.5099696/94‑1 (MQ=60)

                                                  GTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTG                                             >  SRR4302533.1331659/1‑94 (MQ=60)

                                                  GTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTG                                             >  SRR4302533.1515200/1‑94 (MQ=60)

                                                   TTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTATTGATTTTTCGCGCCCTTTGG                                            <  SRR4302533.2560196/94‑1 (MQ=60)

                                                    TTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACTCACGGATGGCGACGAAGCAATGATTATTTGCGCCATTTTGC                                           >  SRR4302533.3022617/1‑94 (MQ=60)

                                                    TTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGC                                           <  SRR4302533.2313142/94‑1 (MQ=60)

                                                       ACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAA                                        <  SRR4302533.4398840/94‑1 (MQ=60)

                                                        CCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAAC                                       >  SRR4302533.2911201/1‑94 (MQ=60)

                                                         CGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACA                                      >  SRR4302533.2244397/1‑94 (MQ=60)

                                                         CGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACA                                      <  SRR4302533.6261053/94‑1 (MQ=60)

                                                          GAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTACCAC                                     >  SRR4302533.1963503/1‑94 (MQ=60)

                                                          GAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACAC                                     <  SRR4302533.243998/94‑1 (MQ=60)

                                                          GAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACAC                                     >  SRR4302533.1388481/1‑94 (MQ=60)

                                                             GCTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAA                                  >  SRR4302533.472393/1‑94 (MQ=60)

                                                              CTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAA                                 >  SRR4302533.3258399/1‑94 (MQ=60)

                                                              CTGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAA                                 >  SRR4302533.2160142/1‑94 (MQ=60)

                                                               TGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAAC                                <  SRR4302533.341195/94‑1 (MQ=60)

                                                               TGGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAAC                                >  SRR4302533.4777706/1‑94 (MQ=60)

                                                                GGCTTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACG                               >  SRR4302533.1733369/1‑94 (MQ=60)

                                                                 GCTTTACCTGCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGT                              <  SRR4302533.963050/94‑1 (MQ=60)

                                                                   TTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTAC                            >  SRR4302533.1266227/1‑94 (MQ=60)

                                                                   TTTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTAC                            <  SRR4302533.2581860/94‑1 (MQ=60)

                                                                    TTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACA                           <  SRR4302533.2898942/94‑1 (MQ=60)

                                                                    TTACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACA                           <  SRR4302533.1839561/94‑1 (MQ=60)

                                                                     TACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACAC                          <  SRR4302533.1037529/94‑1 (MQ=60)

                                                                     TACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACAC                          <  SRR4302533.1326167/94‑1 (MQ=60)

                                                                     TACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACAC                          <  SRR4302533.2156990/94‑1 (MQ=60)

                                                                     TACCTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACAC                          <  SRR4302533.2896015/94‑1 (MQ=60)

                                                                        CTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCA                       >  SRR4302533.2407580/1‑94 (MQ=60)

                                                                        CTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCA                       >  SRR4302533.2372378/1‑94 (MQ=60)

                                                                        CTTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCA                       <  SRR4302533.5137229/94‑1 (MQ=60)

                                                                         TTCCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCAT                      >  SRR4302533.5613190/1‑94 (MQ=60)

                                                                           CCATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAA                    <  SRR4302533.2936833/94‑1 (MQ=60)

                                                                            CATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAAT                   >  SRR4302533.1349028/1‑94 (MQ=60)

                                                                            CATGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAAT                   <  SRR4302533.3746623/94‑1 (MQ=60)

                                                                             ATGCGGCCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATT                  <  SRR4302533.3172533/94‑1 (MQ=60)

                                                                              TGCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTT                 <  SRR4302533.5673484/94‑1 (MQ=60)

                                                                               GCGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTA                <  SRR4302533.6295841/94‑1 (MQ=60)

                                                                                CGGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTAC               <  SRR4302533.1067116/94‑1 (MQ=60)

                                                                                 GGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACT              >  SRR4302533.4798169/1‑94 (MQ=60)

                                                                                 GGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACT              <  SRR4302533.6215780/94‑1 (MQ=60)

                                                                                 GGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACT              >  SRR4302533.4955092/1‑94 (MQ=60)

                                                                                 GGGCAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGA‑TTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTT             <  SRR4302533.2865021/94‑1 (MQ=60)

                                                                                    CAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTCCTTTT           <  SRR4302533.4093257/94‑1 (MQ=60)

                                                                                    CAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTT           <  SRR4302533.2955478/94‑1 (MQ=60)

                                                                                    CAAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTT           >  SRR4302533.4302511/1‑94 (MQ=60)

                                                                                     AAC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTC          <  SRR4302533.5584158/94‑1 (MQ=60)

                                                                                      AC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCT         <  SRR4302533.2930711/94‑1 (MQ=60)

                                                                                      AC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCT         <  SRR4302533.1259920/94‑1 (MQ=60)

                                                                                      AC‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCT         <  SRR4302533.5052679/94‑1 (MQ=60)

                                                                                       C‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTG        <  SRR4302533.2366028/94‑1 (MQ=60)

                                                                                       C‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTG        <  SRR4302533.6032189/94‑1 (MQ=60)

                                                                                       C‑GGCCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTG        <  SRR4302533.1828874/94‑1 (MQ=60)

                                                                                         ggcCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGT       <  SRR4302533.2926175/91‑1 (MQ=60)

                                                                                         ggcCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGT       >  SRR4302533.287040/4‑94 (MQ=60)

                                                                                         GC‑CCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTC      >  SRR4302533.19765/1‑94 (MQ=60)

                                                                                         GC‑CCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTC      <  SRR4302533.805255/94‑1 (MQ=60)

                                                                                          C‑CCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCG     <  SRR4302533.6060828/94‑1 (MQ=60)

                                                                                          C‑CCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCG     >  SRR4302533.5940771/1‑94 (MQ=60)

                                                                                             CCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGAT   <  SRR4302533.1824920/94‑1 (MQ=60)

                                                                                             CCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGAT   <  SRR4302533.1627910/94‑1 (MQ=60)

                                                                                              CCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATG  >  SRR4302533.2194420/1‑94 (MQ=60)

AATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAAC‑GG‑CCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATG  >  NC_000913/1029027‑1029212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`