Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,184,484 A→G F112L (TTT→CTT)  potA ← ATP‑binding component of spermidine/putrescine transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,184,4840AG100.0% 716.4 / NA 223F112L (TTT→CTT) potAATP‑binding component of spermidine/putrescine transport
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (96/127);  total (96/127)

Only 100 of 229 total aligned reads displayed.

GAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGG  >  NC_000913/1184394‑1184573
                                                                                          |                                                                                         
gAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAggg                                                                                         >  2:4419704/1‑93 (MQ=255)
 aGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGc                                                                                        >  2:5118886/1‑93 (MQ=255)
  gCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGcc                                                                                       <  1:4249244/93‑1 (MQ=255)
  gCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGcc                                                                                       >  1:526319/1‑93 (MQ=255)
  gCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGcc                                                                                       <  2:5770958/93‑1 (MQ=255)
   cGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCca                                                                                      <  2:3119029/93‑1 (MQ=255)
    gAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCcac                                                                                     >  1:608592/1‑93 (MQ=255)
     aaGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCcaca                                                                                    >  1:5398971/1‑93 (MQ=255)
     aaGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGGTTTTTGCATGCGCAACCCAAGGGCcaca                                                                                    >  1:3309097/1‑93 (MQ=255)
      aGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACAt                                                                                   <  1:5890459/93‑1 (MQ=255)
        gTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACAttt                                                                                 >  2:1696300/1‑93 (MQ=255)
           tCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCg                                                                              >  1:2859981/1‑93 (MQ=255)
            ccAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGa                                                                             >  1:2762278/1‑93 (MQ=255)
             cAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGaa                                                                            <  1:175259/93‑1 (MQ=255)
             cAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGaa                                                                            >  2:476411/1‑93 (MQ=255)
              aaCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAac                                                                           <  2:4437095/93‑1 (MQ=255)
               aCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAaca                                                                          <  2:5911787/93‑1 (MQ=255)
               aCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAaca                                                                          <  1:3943983/93‑1 (MQ=255)
               aCTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAaca                                                                          >  1:5561003/1‑93 (MQ=255)
                 tGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACg                                                                        >  1:2255314/1‑93 (MQ=255)
                 tGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACg                                                                        >  2:4193005/1‑93 (MQ=255)
                   cACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGt                                                                      <  2:2200199/93‑1 (MQ=255)
                   cACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGt                                                                      <  2:3644088/93‑1 (MQ=255)
                    cccATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTc                                                                     <  1:5134075/92‑1 (MQ=255)
                    aCCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTc                                                                     >  1:5834156/1‑93 (MQ=255)
                    aCCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTc                                                                     >  2:3365748/1‑93 (MQ=255)
                     ccATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCa                                                                    <  2:4154973/93‑1 (MQ=255)
                     ccATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCa                                                                    >  1:4422025/1‑93 (MQ=255)
                     ccATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCa                                                                    <  2:740188/93‑1 (MQ=255)
                     ccATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCa                                                                    <  1:326383/93‑1 (MQ=255)
                      cATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCAt                                                                   <  1:4050435/93‑1 (MQ=255)
                       aTCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCAtg                                                                  >  2:4111532/1‑93 (MQ=255)
                       aTCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCAtg                                                                  >  2:1355025/1‑93 (MQ=255)
                       aTCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCAtg                                                                  <  1:3759320/93‑1 (MQ=255)
                        tCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCAtgt                                                                 >  1:256862/1‑93 (MQ=255)
                        tCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCAtgt                                                                 <  1:1150426/93‑1 (MQ=255)
                          cGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTgg                                                               <  2:1819872/93‑1 (MQ=255)
                           gCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTggg                                                              >  1:4502727/1‑93 (MQ=255)
                           gCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTggg                                                              >  2:6052410/1‑93 (MQ=255)
                           gCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTggg                                                              >  2:3924444/1‑93 (MQ=255)
                            cAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGATTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTgggg                                                             >  1:2151531/1‑93 (MQ=255)
                             aGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTATTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTggggg                                                            >  1:3388021/1‑93 (MQ=255)
                              gggCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCTTGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGa                                                           >  2:3983711/1‑93 (MQ=255)
                              gggCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGa                                                           >  2:5207422/1‑93 (MQ=255)
                              gggCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGa                                                           >  1:3050743/1‑93 (MQ=255)
                              gggCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGa                                                           >  1:3611082/1‑93 (MQ=255)
                               ggCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGaa                                                          >  2:2915207/1‑93 (MQ=255)
                                   tCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGt                                                      >  1:1237039/1‑93 (MQ=255)
                                    ccATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTg                                                     <  1:482218/93‑1 (MQ=255)
                                       tCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGt                                                  <  1:2768764/93‑1 (MQ=255)
                                       tCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGt                                                  <  2:3805041/93‑1 (MQ=255)
                                           gcgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCt                                              <  2:1017789/93‑1 (MQ=255)
                                            cgGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCtt                                             <  1:2489451/93‑1 (MQ=255)
                                             ggggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCttt                                            <  2:2856842/93‑1 (MQ=255)
                                             ggggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCttt                                            <  2:5598394/93‑1 (MQ=255)
                                             ggggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCttt                                            >  2:1854650/1‑93 (MQ=255)
                                             ggggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCttt                                            <  1:2015194/93‑1 (MQ=255)
                                             ggggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCttt                                            <  2:4754693/93‑1 (MQ=255)
                                             ggggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCttt                                            >  1:5646442/1‑93 (MQ=255)
                                             ggggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCttt                                            <  1:3643749/93‑1 (MQ=255)
                                               ggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTgg                                          >  2:6276002/1‑93 (MQ=255)
                                               ggCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTgg                                          <  1:4888027/93‑1 (MQ=255)
                                                gCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGa                                         >  1:4454787/1‑93 (MQ=255)
                                                gCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGa                                         <  2:2545368/93‑1 (MQ=255)
                                                gCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGa                                         <  1:3472974/93‑1 (MQ=255)
                                                  gTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGaaa                                       >  2:890051/1‑93 (MQ=255)
                                                   tAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGaaaa                                      >  2:5683440/1‑93 (MQ=255)
                                                   tAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGaaaa                                      >  1:4130482/1‑93 (MQ=255)
                                                   tAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGaaaa                                      >  1:4974777/1‑93 (MQ=255)
                                                      tttCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAg                                   >  1:1128791/1‑93 (MQ=255)
                                                      tttCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAg                                   <  1:2467399/93‑1 (MQ=255)
                                                        tCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAgtg                                 <  2:2633072/93‑1 (MQ=255)
                                                        tCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAgtg                                 <  1:53696/93‑1 (MQ=255)
                                                         cagcagCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAgtgt                                >  2:5835541/1‑93 (MQ=255)
                                                           gcagcGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTc                              >  1:1473935/1‑93 (MQ=255)
                                                           gcagcGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTc                              >  1:626787/1‑93 (MQ=255)
                                                              gcGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTcaca                           <  2:2164909/93‑1 (MQ=255)
                                                              gcGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTcaca                           <  2:1288622/93‑1 (MQ=255)
                                                              gcGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTcaca                           >  2:4499641/1‑93 (MQ=255)
                                                               cGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACAt                          <  1:1951084/93‑1 (MQ=255)
                                                                gggggTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATa                         <  2:4730805/93‑1 (MQ=255)
                                                                gggggTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATa                         >  2:5559309/1‑93 (MQ=255)
                                                                gggggTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATa                         <  2:2691555/93‑1 (MQ=255)
                                                                 ggggTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGGAAAGTGCGTAGCTTTGGAAAACTGTGTTCACATAg                        >  2:4301956/1‑93 (MQ=255)
                                                                  gggTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGc                       >  2:2288309/1‑93 (MQ=255)
                                                                    gTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCgg                     >  2:6142111/1‑93 (MQ=255)
                                                                     ttttttGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGt                    <  1:888078/93‑1 (MQ=255)
                                                                      tttttGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGtt                   <  1:6199199/93‑1 (MQ=255)
                                                                      tttttGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGtt                   >  2:1455650/1‑93 (MQ=255)
                                                                      tttttGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGtt                   >  1:1594819/1‑93 (MQ=255)
                                                                          tGCATGCGCAACCCAAGGGCCACATTGTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTcc               <  1:5202492/93‑1 (MQ=255)
                                                                             aTGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTccgcc            >  2:5279443/1‑93 (MQ=255)
                                                                               gcgcAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCgg          <  2:4974777/93‑1 (MQ=255)
                                                                                     cccAAGGGCCACACTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTg    >  2:1975614/1‑93 (MQ=255)
                                                                                      ccAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTgg   <  1:1015048/93‑1 (MQ=255)
                                                                                      ccAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTgg   <  2:6107069/93‑1 (MQ=255)
                                                                                      ccAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTgg   <  2:4241210/93‑1 (MQ=255)
                                                                                       cAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAACCAGTGTTCACATAGCGGTTTTCCGCCGGAACGTggg  <  2:3536982/93‑1 (MQ=255)
                                                                                       cAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTggg  >  2:811655/1‑93 (MQ=255)
                                                                                       cAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTggg  >  2:2737006/1‑93 (MQ=255)
                                                                                          |                                                                                         
GAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGG  >  NC_000913/1184394‑1184573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGAT  >  NC_000913/1184391‑1184577
                                                                                             |                                                                                             
GTTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAG                                                                                               <  SRR4302533.2382194/94‑1 (MQ=60)
GTTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAG                                                                                               <  SRR4302533.5154163/94‑1 (MQ=60)
 TTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGG                                                                                              <  SRR4302533.3285881/94‑1 (MQ=60)
 TTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGG                                                                                              <  SRR4302533.5744905/94‑1 (MQ=60)
 TTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGG                                                                                              <  SRR4302533.5674925/94‑1 (MQ=60)
  TGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGG                                                                                             <  SRR4302533.1785948/94‑1 (MQ=60)
  TGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGG                                                                                             <  SRR4302533.3696259/94‑1 (MQ=60)
    AGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCC                                                                                           >  SRR4302533.529437/1‑94 (MQ=60)
     GCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCA                                                                                          <  SRR4302533.4274887/94‑1 (MQ=60)
      CGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCAC                                                                                         >  SRR4302533.612172/1‑94 (MQ=60)
       GAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACA                                                                                        >  SRR4302533.5431415/1‑94 (MQ=60)
       GAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACA                                                                                        <  SRR4302533.2799549/94‑1 (MQ=60)
       GAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGGTTTTTGCATGCGCAACCCAAGGGCCACA                                                                                        >  SRR4302533.3329530/1‑94 (MQ=60)
         AGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATT                                                                                      <  SRR4302533.5926043/94‑1 (MQ=60)
         AGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATT                                                                                      <  SRR4302533.4996498/94‑1 (MQ=60)
          GGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTT                                                                                     <  SRR4302533.3205328/94‑1 (MQ=60)
           GTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTT                                                                                    <  SRR4302533.1455/94‑1 (MQ=60)
             TTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCG                                                                                  >  SRR4302533.2877577/1‑94 (MQ=60)
              TCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGA                                                                                 >  SRR4302533.2779212/1‑94 (MQ=60)
                CAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAAC                                                                               <  SRR4302533.176364/94‑1 (MQ=60)
                CAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAAC                                                                               <  SRR4302533.2750471/94‑1 (MQ=60)
                 AACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACA                                                                              >  SRR4302533.5594609/1‑94 (MQ=60)
                 AACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACA                                                                              <  SRR4302533.302190/94‑1 (MQ=60)
                  ACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACAC                                                                             <  SRR4302533.3968042/94‑1 (MQ=60)
                  ACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACAC                                                                             <  SRR4302533.725617/94‑1 (MQ=60)
                   CTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACG                                                                            >  SRR4302533.2269264/1‑94 (MQ=60)
                   CTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGGTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACG                                                                            >  SRR4302533.1824415/1‑94 (MQ=60)
                     GCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGT                                                                          <  SRR4302533.4238812/94‑1 (MQ=60)
                     GCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGT                                                                          <  SRR4302533.1989427/94‑1 (MQ=60)
                      CACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTC                                                                         >  SRR4302533.3357900/1‑94 (MQ=60)
                      CACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTC                                                                         <  SRR4302533.4339680/94‑1 (MQ=60)
                      CACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTC                                                                         <  SRR4302533.2169732/94‑1 (MQ=60)
                      CACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTC                                                                         >  SRR4302533.5869415/1‑94 (MQ=60)
                       CCCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCA                                                                        <  SRR4302533.5165092/94‑1 (MQ=60)
                       ACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCA                                                                        >  SRR4302533.4448763/1‑94 (MQ=60)
                        CCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCAT                                                                       <  SRR4302533.328369/94‑1 (MQ=60)
                         CATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATG                                                                      <  SRR4302533.4075109/94‑1 (MQ=60)
                          ATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGT                                                                     >  SRR4302533.258428/1‑94 (MQ=60)
                          ATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGT                                                                     <  SRR4302533.5972697/94‑1 (MQ=60)
                          ATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGT                                                                     <  SRR4302533.3782365/94‑1 (MQ=60)
                           TCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTG                                                                    <  SRR4302533.1157317/94‑1 (MQ=60)
                            CCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGG                                                                   >  SRR4302533.4028825/1‑94 (MQ=60)
                             CGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGG                                                                  <  SRR4302533.3328971/94‑1 (MQ=60)
                             CGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGG                                                                  >  SRR4302533.4530006/1‑94 (MQ=60)
                              GCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGATTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGG                                                                 >  SRR4302533.2164783/1‑94 (MQ=60)
                               CAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTATTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGG                                                                >  SRR4302533.3408940/1‑94 (MQ=60)
                                AGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGA                                                               >  SRR4302533.3633308/1‑94 (MQ=60)
                                AGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGA                                                               >  SRR4302533.3069419/1‑94 (MQ=60)
                                  GGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAA                                                             <  SRR4302533.5461587/94‑1 (MQ=60)
                                   GCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAA                                                            <  SRR4302533.1074499/94‑1 (MQ=60)
                                    CTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAG                                                           <  SRR4302533.4510828/94‑1 (MQ=60)
                                    CTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAG                                                           <  SRR4302533.3677634/94‑1 (MQ=60)
                                    CTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAG                                                           >  SRR4302533.208021/1‑94 (MQ=60)
                                     TTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGT                                                          >  SRR4302533.1244430/1‑94 (MQ=60)
                                      TCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGATTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGCGGGGGAAAAGTG                                                         >  SRR4302533.229278/1‑94 (MQ=60)
                                       CCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGC                                                        <  SRR4302533.485100/94‑1 (MQ=60)
                                       CCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGC                                                        <  SRR4302533.1136042/94‑1 (MQ=60)
                                          TCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTA                                                     >  SRR4302533.2415629/1‑94 (MQ=60)
                                          TCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTA                                                     <  SRR4302533.2785754/94‑1 (MQ=60)
                                          TCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTA                                                     <  SRR4302533.2785753/94‑1 (MQ=60)
                                           CACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAG                                                    <  SRR4302533.5285032/94‑1 (MQ=60)
                                             CGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCT                                                  <  SRR4302533.4234677/94‑1 (MQ=60)
                                              GCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACTCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTT                                                 <  SRR4302533.3635920/94‑1 (MQ=60)
                                              GCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACACAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTT                                                 >  SRR4302533.4469263/1‑94 (MQ=60)
                                               CGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTT                                                <  SRR4302533.2504780/94‑1 (MQ=60)
                                               CGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTT                                                >  SRR4302533.5680576/1‑94 (MQ=60)
                                                GGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTG                                               >  SRR4302533.5399060/1‑94 (MQ=60)
                                                GGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTG                                               <  SRR4302533.2027606/94‑1 (MQ=60)
                                                GGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTG                                               <  SRR4302533.3666151/94‑1 (MQ=60)
                                                GGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTG                                               >  SRR4302533.4587176/1‑94 (MQ=60)
                                                  GGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGA                                             >  SRR4302533.4481721/1‑94 (MQ=60)
                                                  GGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGA                                             <  SRR4302533.5417911/94‑1 (MQ=60)
                                                  GGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGA                                             <  SRR4302533.4917644/94‑1 (MQ=60)
                                                   GCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAA                                            <  SRR4302533.3494405/94‑1 (MQ=60)
                                                    CGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAA                                           >  SRR4302533.3488839/1‑94 (MQ=60)
                                                     GTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAA                                          >  SRR4302533.4155553/1‑94 (MQ=60)
                                                     GTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAA                                          >  SRR4302533.5004940/1‑94 (MQ=60)
                                                     GTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAA                                          <  SRR4302533.5174775/94‑1 (MQ=60)
                                                        ATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAG                                       >  SRR4302533.1135497/1‑94 (MQ=60)
                                                        ATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAG                                       <  SRR4302533.3490925/94‑1 (MQ=60)
                                                         TTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGT                                      <  SRR4302533.2482618/94‑1 (MQ=60)
                                                          TTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTG                                     <  SRR4302533.5093355/94‑1 (MQ=60)
                                                           TCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGT                                    <  SRR4302533.54059/94‑1 (MQ=60)
                                                           TCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGT                                    >  SRR4302533.1214800/1‑94 (MQ=60)
                                                            CAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTT                                   >  SRR4302533.3795899/1‑94 (MQ=60)
                                                             AGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTC                                  <  SRR4302533.2587692/94‑1 (MQ=60)
                                                             AGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTC                                  >  SRR4302533.1482842/1‑94 (MQ=60)
                                                             AGCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTC                                  >  SRR4302533.630469/1‑94 (MQ=60)
                                                              GCAGCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCA                                 <  SRR4302533.5140173/94‑1 (MQ=60)
                                                                 GCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACAT                              <  SRR4302533.3505534/94‑1 (MQ=60)
                                                                 GCGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACAT                              <  SRR4302533.5340753/94‑1 (MQ=60)
                                                                  CGGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATA                             <  SRR4302533.1962973/94‑1 (MQ=60)
                                                                   GGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAG                            <  SRR4302533.5917543/94‑1 (MQ=60)
                                                                   GGGGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAG                            >  SRR4302533.1645455/1‑94 (MQ=60)
                                                                     GGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCG                          >  SRR4302533.5778050/1‑94 (MQ=60)
                                                                     GGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCG                          <  SRR4302533.6277955/94‑1 (MQ=60)
                                                                     GGGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCG                          >  SRR4302533.151465/1‑94 (MQ=60)
                                                                      GGTTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGG                         <  SRR4302533.4119837/94‑1 (MQ=60)
                                                                       GTTTTTTGCATGCGCAACCCAAGGACCACATTTTCGAACACGGTCATGTGGTGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGT                        <  SRR4302533.3866962/94‑1 (MQ=60)
                                                                        TTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTT                       <  SRR4302533.893292/94‑1 (MQ=60)
                                                                        TTTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTT                       >  SRR4302533.1604470/1‑94 (MQ=60)
                                                                        TTTCTTGCATGCGCAGCGCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTT                       <  SRR4302533.1873629/94‑1 (MQ=60)
                                                                         TTTTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTT                      <  SRR4302533.6236385/94‑1 (MQ=60)
                                                                           TTTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTC                    >  SRR4302533.5764661/1‑94 (MQ=60)
                                                                            TTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCC                   <  SRR4302533.5628370/94‑1 (MQ=60)
                                                                            TTGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCC                   <  SRR4302533.462105/94‑1 (MQ=60)
                                                                             TGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCG                  <  SRR4302533.6173812/94‑1 (MQ=60)
                                                                             TGCATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGGTTTCCG                  >  SRR4302533.2488570/1‑94 (MQ=60)
                                                                             TGCATGCGCAACCCAAGGGCCACATTGTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCG                  <  SRR4302533.5233845/94‑1 (MQ=60)
                                                                                ATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCG               >  SRR4302533.3161626/1‑94 (MQ=60)
                                                                                ATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCG               <  SRR4302533.6148993/94‑1 (MQ=60)
                                                                                ATGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCG               <  SRR4302533.4867802/94‑1 (MQ=60)
                                                                                 TGCGCAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGG              <  SRR4302533.1392862/94‑1 (MQ=60)
                                                                                     CAACCCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACG          <  SRR4302533.2547024/94‑1 (MQ=60)
                                                                                         CCAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGG      <  SRR4302533.1021009/94‑1 (MQ=60)
                                                                                          CAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGT     >  SRR4302533.3928508/1‑94 (MQ=60)
                                                                                          CAAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGT     >  SRR4302533.2983269/1‑94 (MQ=60)
                                                                                           AAGGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTG    >  SRR4302533.1998968/1‑94 (MQ=60)
                                                                                             GGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGAT  <  SRR4302533.1105595/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTTGAGCGAAGGTTTCCAACTGCACCATCCGCAGGGCTTCCATCACGCGGGGCGTAATTTCAGCAGCGGGGGTTTTTTGCATGCGCAACCCAAAGGCCACATTTTCGAACACGGTCATGTGGGGGAAAAGTGCGTAGCTTTGGAAAACAGTGTTCACATAGCGGTTTTCCGCCGGAACGTGGGTGAT  >  NC_000913/1184391‑1184577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑