Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,230,143	T→C	Y52H (TAC→CAC)	umuD →	UmuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,230,143	0	T	C	100.0%	665.4 / NA	203	Y52H (TAC→CAC)	umuD	UmuD
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (90/113); total (90/113)

Only 100 of 218 total aligned reads displayed.

GTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGC  >  NC_000913/1230053‑1230232

  tCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGactcactt                                                                                       <  2:5083075/93‑1 (MQ=255)

  tCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGactcactt                                                                                       <  2:2762872/93‑1 (MQ=255)

    aGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCg                                                                                     >  1:55279/1‑93 (MQ=255)

       gtgGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCa                                                                                  >  1:2831448/1‑93 (MQ=255)

          gCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAg                                                                               <  1:2222353/93‑1 (MQ=255)

              tCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAg                                                                           >  1:4693977/1‑93 (MQ=255)

              tCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAg                                                                           <  2:6100528/93‑1 (MQ=255)

              tCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAg                                                                           <  2:1153797/93‑1 (MQ=255)

              tCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAg                                                                           <  2:5872418/93‑1 (MQ=255)

               ccTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAgt                                                                          <  2:2038129/93‑1 (MQ=255)

               ccTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAgg                                                                          >  1:83633/1‑92 (MQ=255)

               ccTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAgg                                                                          >  2:2606223/1‑92 (MQ=255)

                cTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAgtg                                                                         <  2:1249825/93‑1 (MQ=255)

                  tCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAgtggt                                                                       >  2:5986341/1‑93 (MQ=255)

                  tCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAgtggt                                                                       <  1:1801768/93‑1 (MQ=255)

                  tCACCGGCAGAAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAgtggt                                                                       <  1:6114791/93‑1 (MQ=255)

                    aCCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGa                                                                     <  1:3808474/93‑1 (MQ=255)

                    aCCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGa                                                                     <  1:1007114/93‑1 (MQ=255)

                     ccGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGAt                                                                    >  1:6169485/1‑93 (MQ=255)

                     ccGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGAt                                                                    >  2:3991794/1‑93 (MQ=255)

                     ccGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGAt                                                                    <  2:5466317/93‑1 (MQ=255)

                         cagcagATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTa                                                                <  2:25529/93‑1 (MQ=255)

                           gcagATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTAtg                                                              >  2:6229021/1‑93 (MQ=255)

                             agATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTAtgat                                                            >  2:5694755/1‑93 (MQ=255)

                              gATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTAtgatt                                                           >  1:5963676/1‑93 (MQ=255)

                              gATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTAtgatt                                                           <  1:51163/93‑1 (MQ=255)

                                ttACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTAtgattga                                                         <  1:3623474/93‑1 (MQ=255)

                                 tACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTAtgattgat                                                        <  1:2944098/93‑1 (MQ=255)

                                  aCGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtg                                                       >  2:3952161/1‑93 (MQ=255)

                                  aCGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtg                                                       <  1:2277386/93‑1 (MQ=255)

                                  aCGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtg                                                       <  1:32753/93‑1 (MQ=255)

                                   cGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtgg                                                      <  2:3046154/93‑1 (MQ=255)

                                   cGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtgg                                                      <  2:5581807/93‑1 (MQ=255)

                                   cGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtgg                                                      <  1:890166/93‑1 (MQ=255)

                                    gTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtggt                                                     <  1:2990745/93‑1 (MQ=255)

                                      tGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtggtgg                                                   <  1:1408372/93‑1 (MQ=255)

                                         aCAGCGCATAGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAAt                                                >  2:4262863/1‑93 (MQ=255)

                                          cAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAAtt                                               >  1:972169/1‑93 (MQ=255)

                                           aGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTa                                              >  1:5252093/1‑93 (MQ=255)

                                             cgcATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGt                                            <  2:1473961/93‑1 (MQ=255)

                                             cgcATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGt                                            <  1:6001469/93‑1 (MQ=255)

                                               cATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGa                                          <  1:1857206/93‑1 (MQ=255)

                                               cATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGa                                          <  1:4278435/93‑1 (MQ=255)

                                               cATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGa                                          <  1:2359709/93‑1 (MQ=255)

                                                aTCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAc                                         <  2:1614253/93‑1 (MQ=255)

                                                aTCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAc                                         >  2:5250291/1‑93 (MQ=255)

                                                aTCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAc                                         >  1:4780796/1‑93 (MQ=255)

                                                aTCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAc                                         <  2:4948830/93‑1 (MQ=255)

                                                   gATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGt                                      >  1:5770453/1‑93 (MQ=255)

                                                   gATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGt                                      >  2:2937464/1‑93 (MQ=255)

                                                   gATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGt                                      >  2:4166173/1‑93 (MQ=255)

                                                    aTCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTg                                     >  2:2375155/1‑93 (MQ=255)

                                                    aTCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTg                                     >  2:3193997/1‑93 (MQ=255)

                                                    aTCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTg                                     <  2:798950/93‑1 (MQ=255)

                                                    aTCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTg                                     >  2:1779896/1‑93 (MQ=255)

                                                     tCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGa                                    >  2:6261655/1‑93 (MQ=255)

                                                     tCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGa                                    >  2:2143571/1‑93 (MQ=255)

                                                     tCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGa                                    >  1:5318136/1‑93 (MQ=255)

                                                      cTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCACAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGAt                                   >  1:4641237/1‑93 (MQ=255)

                                                      cTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGAt                                   <  1:1509847/93‑1 (MQ=255)

                                                      cTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGAt                                   >  1:3228543/1‑93 (MQ=255)

                                                         aaTCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTa                                <  1:2682365/93‑1 (MQ=255)

                                                          aTCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTAc                               >  2:4683434/1‑93 (MQ=255)

                                                          aTCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTAc                               >  2:5164176/1‑93 (MQ=255)

                                                           tCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACt                              <  2:3213961/93‑1 (MQ=255)

                                                              aCTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGAt                           <  1:6010677/93‑1 (MQ=255)

                                                               cTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGAtt                          >  2:4783767/1‑93 (MQ=255)

                                                                tGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTg                         >  2:3742992/1‑93 (MQ=255)

                                                                tGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTg                         <  2:2215410/93‑1 (MQ=255)

                                                                tGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTg                         <  1:3563836/93‑1 (MQ=255)

                                                                 gTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGt                        <  2:1674627/93‑1 (MQ=255)

                                                                   tGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCg                      >  1:4977901/1‑93 (MQ=255)

                                                                    gATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGa                     >  1:5896055/1‑93 (MQ=255)

                                                                    gATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGa                     <  1:640779/93‑1 (MQ=255)

                                                                    gATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGa                     <  2:3455111/93‑1 (MQ=255)

                                                                    gATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGa                     >  1:410244/1‑93 (MQ=255)

                                                                    gATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGa                     >  2:646361/1‑93 (MQ=255)

                                                                     aTCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGAt                    <  1:3912644/93‑1 (MQ=255)

                                                                     aTCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGAt                    >  1:3661413/1‑93 (MQ=255)

                                                                      tCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATa                   >  1:5689803/1‑93 (MQ=255)

                                                                       ccAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAg                  >  2:4596471/1‑93 (MQ=255)

                                                                        cAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAgc                 >  2:5305999/1‑93 (MQ=255)

                                                                         aGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAgcg                >  2:1821619/1‑93 (MQ=255)

                                                                         aGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAgcg                >  2:1375491/1‑93 (MQ=255)

                                                                          gCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAgcgc               <  1:1907573/93‑1 (MQ=255)

                                                                             tCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTAt            <  2:4513049/93‑1 (MQ=255)

                                                                             tCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTAt            >  1:3102971/1‑93 (MQ=255)

                                                                             tCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTAt            <  1:1977899/93‑1 (MQ=255)

                                                                              cccAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTAtt           <  2:312023/93‑1 (MQ=255)

                                                                              cccAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTAtt           <  2:5900576/93‑1 (MQ=255)

                                                                               ccAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTa          <  2:4023300/93‑1 (MQ=255)

                                                                                cAGCGCGACTCACTTCGTCAAAGCAAGTGGCGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGCTTGTCGATAGCGCTATTAc         >  2:2456093/1‑93 (MQ=255)

                                                                                 aGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTAcc        >  1:3775522/1‑93 (MQ=255)

                                                                                 aGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTAcc        <  1:4964013/93‑1 (MQ=255)

                                                                                 aGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTAcc        <  2:1973420/93‑1 (MQ=255)

                                                                                   cgcgACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCgc      >  2:753449/1‑93 (MQ=255)

                                                                                    gcgACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCgcc     >  1:1330767/1‑93 (MQ=255)

                                                                                    gcgACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCgcc     >  1:4672235/1‑93 (MQ=255)

                                                                                      gACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCgccag   <  1:158065/93‑1 (MQ=255)

                                                                                       actcacttCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCgccagc  >  2:4643462/1‑93 (MQ=255)

GTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGC  >  NC_000913/1230053‑1230232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATG  >  NC_000913/1230050‑1230236

CTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTC                                                                                               >  SRR4302533.3273062/1‑94 (MQ=60)

CTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTC                                                                                               >  SRR4302533.163263/1‑94 (MQ=60)

 TTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCA                                                                                              <  SRR4302533.4615528/94‑1 (MQ=60)

 TTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCA                                                                                              <  SRR4302533.2212033/94‑1 (MQ=60)

  TGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCAC                                                                                             <  SRR4302533.93125/94‑1 (MQ=60)

   GTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGGATCAACTGTTGATCCAGCATCCCAGCGCGACTCACT                                                                                            >  SRR4302533.2608783/1‑94 (MQ=60)

    TTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTT                                                                                           >  SRR4302533.3850265/1‑94 (MQ=60)

     TCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTC                                                                                          <  SRR4302533.4699064/94‑1 (MQ=60)

      CAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCG                                                                                         >  SRR4302533.55651/1‑94 (MQ=60)

       AGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGT                                                                                        >  SRR4302533.1850161/1‑94 (MQ=60)

         TGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCA                                                                                      >  SRR4302533.2848870/1‑94 (MQ=60)

            GGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAG                                                                                   <  SRR4302533.5341700/94‑1 (MQ=60)

            GGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAG                                                                                   <  SRR4302533.4977810/94‑1 (MQ=60)

            GGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAG                                                                                   >  SRR4302533.3809773/1‑94 (MQ=60)

             GCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGC                                                                                  <  SRR4302533.2236081/94‑1 (MQ=60)

               TTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAA                                                                                >  SRR4302533.4709766/1‑94 (MQ=60)

                TTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAG                                                                               <  SRR4302533.6043870/94‑1 (MQ=60)

                TTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAG                                                                               >  SRR4302533.4722421/1‑94 (MQ=60)

                 TCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCGAAGCATGT                                                                              >  SRR4302533.3143271/1‑94 (MQ=60)

                 TCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGG                                                                              >  SRR4302533.639242/1‑94 (MQ=60)

                 TCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGG                                                                              >  SRR4302533.84166/1‑94 (MQ=60)

                  CCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTG                                                                             <  SRR4302533.2563561/94‑1 (MQ=60)

                    TTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGT                                                                           <  SRR4302533.2712452/94‑1 (MQ=60)

                     TCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTG                                                                          <  SRR4302533.620112/94‑1 (MQ=60)

                     TCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTG                                                                          <  SRR4302533.5912638/94‑1 (MQ=60)

                     TCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTG                                                                          <  SRR4302533.1812733/94‑1 (MQ=60)

                     TCACCGGCAGAAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTG                                                                          <  SRR4302533.6151686/94‑1 (MQ=60)

                      CACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGA                                                                         >  SRR4302533.3273764/1‑94 (MQ=60)

                       ACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGAT                                                                        >  SRR4302533.6206569/1‑94 (MQ=60)

                       ACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGAT                                                                        <  SRR4302533.1013006/94‑1 (MQ=60)

                       ACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGAT                                                                        <  SRR4302533.3831772/94‑1 (MQ=60)

                          GGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCT                                                                     >  SRR4302533.3645020/1‑94 (MQ=60)

                          GGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCT                                                                     >  SRR4302533.1878334/1‑94 (MQ=60)

                                AGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATT                                                               >  SRR4302533.5999755/1‑94 (MQ=60)

                                 GATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTG                                                              <  SRR4302533.3196521/94‑1 (MQ=60)

                                 GATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTG                                                              <  SRR4302533.51511/94‑1 (MQ=60)

                                   TTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAT                                                            <  SRR4302533.3645773/94‑1 (MQ=60)

                                   TTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAT                                                            <  SRR4302533.1813376/94‑1 (MQ=60)

                                    TACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATG                                                           <  SRR4302533.2962067/94‑1 (MQ=60)

                                     ACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGG                                                          <  SRR4302533.32975/94‑1 (MQ=60)

                                     ACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGG                                                          >  SRR4302533.5083321/1‑94 (MQ=60)

                                     ACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGG                                                          <  SRR4302533.2291489/94‑1 (MQ=60)

                                      CGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGT                                                         <  SRR4302533.1242654/94‑1 (MQ=60)

                                      CGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGT                                                         <  SRR4302533.895396/94‑1 (MQ=60)

                                       GTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTG                                                        <  SRR4302533.3009037/94‑1 (MQ=60)

                                         TGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGA                                                      <  SRR4302533.1416885/94‑1 (MQ=60)

                                         TGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGA                                                      <  SRR4302533.1625674/94‑1 (MQ=60)

                                          GAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGaggag                                                     >  SRR4302533.2935227/1‑89 (MQ=60)

                                          GAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAA                                                     <  SRR4302533.5161521/94‑1 (MQ=60)

                                            ACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATT                                                   >  SRR4302533.977878/1‑94 (MQ=60)

                                            ACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATT                                                   >  SRR4302533.3759422/1‑94 (MQ=60)

                                             CAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTA                                                  >  SRR4302533.5283650/1‑94 (MQ=60)

                                                CGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTG                                               <  SRR4302533.6037825/94‑1 (MQ=60)

                                                  CATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAC                                             <  SRR4302533.1868533/94‑1 (MQ=60)

                                                  CATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAC                                             <  SRR4302533.2374292/94‑1 (MQ=60)

                                                  CATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAC                                             >  SRR4302533.4809762/1‑94 (MQ=60)

                                                  CATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGAC                                             <  SRR4302533.4304164/94‑1 (MQ=60)

                                                   ATCGATCTGAATCAACTGTTGATCCAGCATCACAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACG                                            <  SRR4302533.3804017/94‑1 (MQ=60)

                                                     CGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGT                                          >  SRR4302533.5805294/1‑94 (MQ=60)

                                                     CGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGT                                          <  SRR4302533.309315/94‑1 (MQ=60)

                                                       ATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGA                                        >  SRR4302533.6013859/1‑94 (MQ=60)

                                                       ATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGA                                        >  SRR4302533.5350006/1‑94 (MQ=60)

                                                        TCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCACAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGAT                                       >  SRR4302533.4669357/1‑94 (MQ=60)

                                                        TCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGAT                                       >  SRR4302533.895646/1‑94 (MQ=60)

                                                        TCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGAT                                       >  SRR4302533.3248445/1‑94 (MQ=60)

                                                         CTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATT                                      <  SRR4302533.1518948/94‑1 (MQ=60)

                                                          TGAATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTT                                     <  SRR4302533.4079612/94‑1 (MQ=60)

                                                            AATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTAC                                   <  SRR4302533.74637/94‑1 (MQ=60)

                                                            AATCAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTAC                                   <  SRR4302533.2698802/94‑1 (MQ=60)

                                                               CAACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGA                                <  SRR4302533.6226790/94‑1 (MQ=60)

                                                                AACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGCT                               <  SRR4302533.5281276/94‑1 (MQ=60)

                                                                 ACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATT                              <  SRR4302533.6047092/94‑1 (MQ=60)

                                                                 ACTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGATGATTTACTGATT                              <  SRR4302533.536436/94‑1 (MQ=60)

                                                                  CTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTG                             >  SRR4302533.3676207/1‑94 (MQ=60)

                                                                  CTGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTG                             <  SRR4302533.1524448/94‑1 (MQ=60)

                                                                   TGTTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGT                            <  SRR4302533.3585796/94‑1 (MQ=60)

                                                                     TTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCG                          >  SRR4302533.5008086/1‑94 (MQ=60)

                                                                     TTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCG                          <  SRR4302533.5817293/94‑1 (MQ=60)

                                                                     TTGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCG                          <  SRR4302533.6134447/94‑1 (MQ=60)

                                                                      TGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGA                         >  SRR4302533.5931659/1‑94 (MQ=60)

                                                                      TGATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGA                         >  SRR4302533.412703/1‑94 (MQ=60)

                                                                       GATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGAT                        <  SRR4302533.2998012/94‑1 (MQ=60)

                                                                       GATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGAT                        <  SRR4302533.5640274/94‑1 (MQ=60)

                                                                       GATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGAT                        <  SRR4302533.644546/94‑1 (MQ=60)

                                                                       GATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGAT                        >  SRR4302533.3683925/1‑94 (MQ=60)

                                                                       GATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGAT                        <  SRR4302533.499473/94‑1 (MQ=60)

                                                                       GATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGAT                        <  SRR4302533.2262720/94‑1 (MQ=60)

                                                                        ATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATA                       <  SRR4302533.3936540/94‑1 (MQ=60)

                                                                        ATCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATA                       >  SRR4302533.5724169/1‑94 (MQ=60)

                                                                         TCCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAG                      <  SRR4302533.1358279/94‑1 (MQ=60)

                                                                          CCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGC                     >  SRR4302533.348186/1‑94 (MQ=60)

                                                                          CCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGC                     <  SRR4302533.2353577/94‑1 (MQ=60)

                                                                          CCAGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGC                     <  SRR4302533.5778482/94‑1 (MQ=60)

                                                                            AGCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGC                   >  SRR4302533.4679238/1‑94 (MQ=60)

                                                                             GCATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCT                  <  SRR4302533.1919211/94‑1 (MQ=60)

                                                                               ATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTAT                >  SRR4302533.3122050/1‑94 (MQ=60)

                                                                               ATCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTAT                <  SRR4302533.4744526/94‑1 (MQ=60)

                                                                                TCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATT               <  SRR4302533.861461/94‑1 (MQ=60)

                                                                                TCCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATT               <  SRR4302533.1990011/94‑1 (MQ=60)

                                                                                 CCCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTA              <  SRR4302533.1440876/94‑1 (MQ=60)

                                                                                  CCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTAC             <  SRR4302533.4266741/94‑1 (MQ=60)

                                                                                  CCAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTAC             >  SRR4302533.221995/1‑94 (MQ=60)

                                                                                   CAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACC            >  SRR4302533.3798665/1‑94 (MQ=60)

                                                                                   CAGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACC            <  SRR4302533.1947227/94‑1 (MQ=60)

                                                                                    AGCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCG           <  SRR4302533.4994107/94‑1 (MQ=60)

                                                                                     GCGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGC          <  SRR4302533.5993569/94‑1 (MQ=60)

                                                                                      CGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCC         >  SRR4302533.1338751/1‑94 (MQ=60)

                                                                                      CGCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCC         >  SRR4302533.4700547/1‑94 (MQ=60)

                                                                                       GCGACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCA        >  SRR4302533.4493570/1‑94 (MQ=60)

                                                                                         GACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGC      <  SRR4302533.159073/94‑1 (MQ=60)

                                                                                          ACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCC     >  SRR4302533.2978063/1‑94 (MQ=60)

                                                                                          ACTCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCC     >  SRR4302533.5641117/1‑94 (MQ=60)

                                                                                           CTCACTTCGTCAAAGCAAGTGGTGATTCTATGATCGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCA    >  SRR4302533.6179565/1‑94 (MQ=60)

                                                                                            TCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCAT   <  SRR4302533.5399018/94‑1 (MQ=60)

                                                                                            TCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCAT   <  SRR4302533.535713/94‑1 (MQ=60)

                                                                                            TCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCAT   <  SRR4302533.3855875/94‑1 (MQ=60)

                                                                                            TCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCAT   <  SRR4302533.4955467/94‑1 (MQ=60)

                                                                                            TCACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCAT   <  SRR4302533.1089484/94‑1 (MQ=60)

                                                                                             CACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATG  >  SRR4302533.521428/1‑94 (MQ=60)

CTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGGTGGAATTAGTGACGGTGATTTACTGATTGTCGATAGCGCTATTACCGCCAGCCATG  >  NC_000913/1230050‑1230236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`