Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,230,972	A→G	G188G (GGA→GGG)	umuC →	UmuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,230,972	0	A	G	99.6%	841.8 / ‑5.2	263	G188G (GGA→GGG)	umuC	UmuC
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (147/115); total (148/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

Only 100 of 277 total aligned reads displayed.

GCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTT  >  NC_000913/1230882‑1231061

  aGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGggcggc                                                                                       >  2:1022735/1‑93 (MQ=255)

   gACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGgcggcg                                                                                      >  2:9890554/1‑93 (MQ=255)

   gACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGgcggcg                                                                                      >  2:8876221/1‑93 (MQ=255)

   gACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGgcggcg                                                                                      >  2:509397/1‑93 (MQ=255)

   gACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGgcggcg                                                                                      <  2:1277303/93‑1 (MQ=255)

     ggggtggggtgGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGa                                                                                    <  2:57274/92‑1 (MQ=255)

       ggtggggtggTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATc                                                                                  >  2:8302783/1‑93 (MQ=255)

          ggggtggTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGc                                                                               <  2:6073942/93‑1 (MQ=255)

          ggggtggTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGAGGATCAGc                                                                               >  1:386175/1‑93 (MQ=255)

              tggtggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCaaaa                                                                           >  1:5168317/1‑93 (MQ=255)

               ggtggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCaaaaa                                                                          >  1:6655351/1‑93 (MQ=255)

                gtggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCaaaaaa                                                                         >  1:7665333/1‑93 (MQ=255)

                gtggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCaaaaaa                                                                         >  1:4341403/1‑93 (MQ=255)

                gtggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCaaaaaa                                                                         >  1:319087/1‑93 (MQ=255)

                 tggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGATGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAAc                                                                        <  1:1261648/93‑1 (MQ=255)

                 tggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAAc                                                                        <  1:6255379/93‑1 (MQ=255)

                  ggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACt                                                                       >  2:9400039/1‑93 (MQ=255)

                   gATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTg                                                                      <  2:2791816/93‑1 (MQ=255)

                   gATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTg                                                                      <  1:5651792/93‑1 (MQ=255)

                   gATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTATGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTg                                                                      <  1:9203781/93‑1 (MQ=255)

                    aTTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTgg                                                                     <  1:9884966/93‑1 (MQ=255)

                     tttATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGa                                                                    <  1:165199/93‑1 (MQ=255)

                     tttATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGa                                                                    <  1:8892730/93‑1 (MQ=255)

                     tttATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGa                                                                    >  1:5288490/1‑93 (MQ=255)

                       tttCAAATCTGGAACGCCAGCGGAAATTAATGTCTGCTCTCCCCGTGGGTGACGTCTGGGGGATTTGGCGGCCGATCAGCAAAAAACTGGagg                                                                  >  2:6141232/3‑91 (MQ=255)

                       tATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGAcg                                                                  >  1:1013599/1‑93 (MQ=255)

                        aTCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGAcgc                                                                 <  2:8508212/93‑1 (MQ=255)

                          cAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGa                                                               <  1:8298242/93‑1 (MQ=255)

                          cAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGa                                                               >  2:7691743/1‑93 (MQ=255)

                          cAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGa                                                               <  1:9358034/93‑1 (MQ=255)

                           aaaTCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGAt                                                              >  1:4132067/1‑93 (MQ=255)

                            aaTCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATg                                                             >  2:9161707/1‑93 (MQ=255)

                            aaTCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATg                                                             <  2:5368112/93‑1 (MQ=255)

                               cTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATgggg                                                          >  1:5085299/1‑93 (MQ=255)

                                 ggAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGAt                                                        <  1:5769921/93‑1 (MQ=255)

                                  gAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATc                                                       >  1:6769624/1‑93 (MQ=255)

                                   aaCGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCa                                                      <  2:9516277/93‑1 (MQ=255)

                                   aaCGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCa                                                      >  2:7072939/1‑93 (MQ=255)

                                    aCGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCaa                                                     >  1:2016231/1‑93 (MQ=255)

                                     cGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATTACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCaaa                                                    >  1:2763210/1‑93 (MQ=255)

                                     cGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCaaa                                                    <  1:8170844/93‑1 (MQ=255)

                                     cGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCaaa                                                    <  1:6187436/93‑1 (MQ=255)

                                      gCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCaaaa                                                   >  1:2505267/1‑93 (MQ=255)

                                        cAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAAcc                                                 >  1:1418840/1‑93 (MQ=255)

                                          gCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGt                                               >  1:6600576/1‑93 (MQ=255)

                                           cGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGtt                                              <  1:2553080/93‑1 (MQ=255)

                                           cGTAAATTAATAGCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGGCGCGATGGGGATCAAAACCGtt                                              <  2:4147048/93‑1 (MQ=255)

                                            gTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTtc                                             <  2:5547243/93‑1 (MQ=255)

                                              aaaTTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTtctc                                           >  1:2843785/1‑93 (MQ=255)

                                               aaTTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCg                                          >  2:4408065/1‑93 (MQ=255)

                                                  tAATGGCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGAtt                                       <  1:8556071/93‑1 (MQ=255)

                                                   aaTGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGAttt                                      >  2:3462848/1‑93 (MQ=255)

                                                    aTGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTg                                     >  1:9210769/1‑93 (MQ=255)

                                                     tGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTgg                                    >  1:1731012/1‑93 (MQ=255)

                                                      gTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGc                                   <  2:6240798/93‑1 (MQ=255)

                                                      gTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGc                                   >  1:4667219/1‑93 (MQ=255)

                                                       tCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCg                                  >  2:3826926/1‑93 (MQ=255)

                                                        cTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCgg                                 >  1:6432937/1‑93 (MQ=255)

                                                        cTGCTCTCCCAGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGTATCAAAACCGTCCTCGATTTGGCgg                                 <  2:6511397/93‑1 (MQ=255)

                                                         tGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTggagga                                >  2:1587100/1‑93 (MQ=255)

                                                         tGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGa                                <  2:6593940/93‑1 (MQ=255)

                                                         tGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGa                                <  2:3197928/93‑1 (MQ=255)

                                                           ctctCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATa                              >  1:8017101/1‑93 (MQ=255)

                                                            tctcCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATAc                             <  2:6720148/93‑1 (MQ=255)

                                                               ccccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGa                          >  2:7644832/1‑93 (MQ=255)

                                                               ccccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGa                          >  2:4703674/1‑93 (MQ=255)

                                                                cccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGat                         <  2:4306243/93‑1 (MQ=255)

                                                                cccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGat                         >  1:3369093/1‑93 (MQ=255)

                                                                cccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGat                         <  1:8131984/93‑1 (MQ=255)

                                                                cccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGat                         >  2:9777596/1‑93 (MQ=255)

                                                                 ccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGata                        <  2:3008622/93‑1 (MQ=255)

                                                                 ccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGata                        <  1:7292231/93‑1 (MQ=255)

                                                                 ccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGata                        >  1:3976038/1‑93 (MQ=255)

                                                                  cGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGTTACAGatat                       >  1:9042226/1‑93 (MQ=255)

                                                                  cGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTAGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGatat                       >  2:2873983/1‑93 (MQ=255)

                                                                   gTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATc                      >  2:7771613/1‑93 (MQ=255)

                                                                   gTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATc                      <  1:7813395/93‑1 (MQ=255)

                                                                    tGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATcc                     >  1:3686518/1‑93 (MQ=255)

                                                                    tGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATcc                     >  2:3662945/1‑93 (MQ=255)

                                                                      gATGCCGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCgg                   <  2:2219829/93‑1 (MQ=255)

                                                                      gATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCgg                   >  2:1640583/1‑93 (MQ=255)

                                                                       aTGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGt                  <  1:3004758/93‑1 (MQ=255)

                                                                        tGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGAACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGtt                 >  2:1710232/1‑93 (MQ=255)

                                                                          aCGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTa               <  2:9564752/93‑1 (MQ=255)

                                                                            gTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATc             <  1:7242472/93‑1 (MQ=255)

                                                                            gTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATc             <  1:1385517/93‑1 (MQ=255)

                                                                            gTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATc             <  1:2112113/93‑1 (MQ=255)

                                                                             tcggggggATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATcc            <  2:7373605/90‑1 (MQ=255)

                                                                               tGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATCCAGATATCCGGTTTATCTGt          >  1:7521882/1‑93 (MQ=255)

                                                                               tGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGt          >  2:8112721/1‑93 (MQ=255)

                                                                               tGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGt          >  1:8781893/1‑93 (MQ=255)

                                                                               tGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGt          <  2:309803/93‑1 (MQ=255)

                                                                                gggggATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTa         <  1:4630885/93‑1 (MQ=255)

                                                                                 ggggATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTaa        >  2:5191844/1‑93 (MQ=255)

                                                                                   ggATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAAc      >  2:2327681/1‑93 (MQ=255)

                                                                                    gATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACa     >  2:4070844/1‑93 (MQ=255)

                                                                                    gATTGGGCCGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACa     <  2:8651576/93‑1 (MQ=255)

                                                                                     aTTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAATCCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACAt    <  1:2282251/93‑1 (MQ=255)

                                                                                      ttGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACAtt   >  2:5929888/1‑93 (MQ=255)

                                                                                       tGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACAttt  <  2:2031118/93‑1 (MQ=255)

GCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTT  >  NC_000913/1230882‑1231061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTTTAAT  >  NC_000913/1230880‑1231065

CGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGC                                                                                              <  SRR4302540.7306814/94‑1 (MQ=60)

CGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGC                                                                                              <  SRR4302540.1679801/94‑1 (MQ=60)

CGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGC                                                                                              <  SRR4302540.1679800/94‑1 (MQ=60)

 GGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCG                                                                                             >  SRR4302540.6613631/1‑94 (MQ=60)

        GGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATC                                                                                      >  SRR4302540.8816337/1‑94 (MQ=60)

        GGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATC                                                                                      >  SRR4302540.7671566/1‑94 (MQ=60)

        GGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATC                                                                                      >  SRR4302540.9660534/1‑94 (MQ=60)

          GTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAG                                                                                    >  SRR4302540.9295052/1‑94 (MQ=60)

          GTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAG                                                                                    >  SRR4302540.8980733/1‑94 (MQ=60)

           TGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGC                                                                                   <  SRR4302540.468236/94‑1 (MQ=60)

           TGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGAGGATCAGC                                                                                   >  SRR4302540.390169/1‑94 (MQ=60)

            GGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCA                                                                                  <  SRR4302540.2956279/94‑1 (MQ=60)

             GGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAA                                                                                 >  SRR4302540.2809910/1‑94 (MQ=60)

              GGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAA                                                                                >  SRR4302540.8242098/1‑94 (MQ=60)

              GGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAA                                                                                >  SRR4302540.3814313/1‑94 (MQ=60)

               GTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAA                                                                               >  SRR4302540.5224535/1‑94 (MQ=60)

                TGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAA                                                                              >  SRR4302540.6728956/1‑94 (MQ=60)

                TGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAA                                                                              >  SRR4302540.3493379/1‑94 (MQ=60)

                TGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAA                                                                              >  SRR4302540.2076683/1‑94 (MQ=60)

                 GGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAA                                                                             >  SRR4302540.7751686/1‑94 (MQ=60)

                 GGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAA                                                                             >  SRR4302540.322273/1‑94 (MQ=60)

                 GGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAA                                                                             >  SRR4302540.3704433/1‑94 (MQ=60)

                 GGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAA                                                                             >  SRR4302540.4388666/1‑94 (MQ=60)

                  GTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAAC                                                                            <  SRR4302540.2534677/94‑1 (MQ=60)

                  GTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAAC                                                                            <  SRR4302540.8320092/94‑1 (MQ=60)

                   TGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGATGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACT                                                                           <  SRR4302540.1274595/94‑1 (MQ=60)

                   TGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACT                                                                           <  SRR4302540.6324979/94‑1 (MQ=60)

                   TGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACT                                                                           >  SRR4302540.4521235/1‑94 (MQ=60)

                     GATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGG                                                                         <  SRR4302540.5714019/94‑1 (MQ=60)

                     GATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTATGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGG                                                                         <  SRR4302540.9314214/94‑1 (MQ=60)

                     GATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTATGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGG                                                                         <  SRR4302540.9314216/94‑1 (MQ=60)

                      ATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGC                                                                        >  SRR4302540.3211910/1‑94 (MQ=60)

                      ATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGA                                                                        >  SRR4302540.5346291/1‑94 (MQ=60)

                      ATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGA                                                                        <  SRR4302540.10000388/94‑1 (MQ=60)

                       TTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGAC                                                                       <  SRR4302540.166851/94‑1 (MQ=60)

                       TTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGAC                                                                       <  SRR4302540.8995673/94‑1 (MQ=60)

                        TTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACG                                                                      >  SRR4302540.1023874/1‑94 (MQ=60)

                           TCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGA                                                                   >  SRR4302540.935171/1‑94 (MQ=60)

                            CAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGgcaacaactggagccgtg                                                                  >  SRR4302540.7818908/1‑76 (MQ=60)

                            CAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGAT                                                                  <  SRR4302540.9469485/94‑1 (MQ=60)

                            CAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGAT                                                                  <  SRR4302540.5218282/94‑1 (MQ=60)

                            CAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGAT                                                                  >  SRR4302540.4176760/1‑94 (MQ=60)

                            CAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGAT                                                                  <  SRR4302540.8394595/94‑1 (MQ=60)

                            CAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGAT                                                                  <  SRR4302540.329232/94‑1 (MQ=60)

                            CAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGAG                                                                  >  SRR4302540.7818904/1‑94 (MQ=60)

                             AAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCACAAAACTGGACGCGATG                                                                 >  SRR4302540.8429458/1‑94 (MQ=60)

                             AAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATG                                                                 >  SRR4302540.8429457/1‑94 (MQ=60)

                             AAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATG                                                                 >  SRR4302540.5318585/1‑94 (MQ=60)

                              AATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGG                                                                <  SRR4302540.1558642/94‑1 (MQ=60)

                               ATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGG                                                               >  SRR4302540.684066/1‑94 (MQ=60)

                               ATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGG                                                               >  SRR4302540.2540991/1‑94 (MQ=60)

                                TCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGG                                                              >  SRR4302540.5317654/1‑94 (MQ=60)

                                TCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGG                                                              <  SRR4302540.6350547/94‑1 (MQ=60)

                                TCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGG                                                              >  SRR4302540.5140276/1‑94 (MQ=60)

                                 CTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGA                                                             >  SRR4302540.2268201/1‑94 (MQ=60)

                                   GGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATC                                                           >  SRR4302540.6844436/1‑94 (MQ=60)

                                   GGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATC                                                           <  SRR4302540.5833649/94‑1 (MQ=60)

                                     AACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAA                                                         >  SRR4302540.2038029/1‑94 (MQ=60)

                                      ACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATTACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAA                                                        >  SRR4302540.2793062/1‑94 (MQ=60)

                                      ACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAA                                                        <  SRR4302540.7948362/94‑1 (MQ=60)

                                      ACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAA                                                        >  SRR4302540.5938183/1‑94 (MQ=60)

                                       CGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAA                                                       <  SRR4302540.6256337/94‑1 (MQ=60)

                                       CGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAA                                                       >  SRR4302540.2532311/1‑94 (MQ=60)

                                       CGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAA                                                       <  SRR4302540.8265738/94‑1 (MQ=60)

                                        GCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAAC                                                      <  SRR4302540.7575367/94‑1 (MQ=60)

                                         CCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACC                                                     >  SRR4302540.1434182/1‑94 (MQ=60)

                                          CAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCG                                                    >  SRR4302540.1371266/1‑94 (MQ=60)

                                          CAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCG                                                    >  SRR4302540.1584950/1‑94 (MQ=60)

                                          CAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCG                                                    >  SRR4302540.400075/1‑94 (MQ=60)

                                           AGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGT                                                   >  SRR4302540.6673667/1‑94 (MQ=60)

                                           AGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGT                                                   <  SRR4302540.1424098/94‑1 (MQ=60)

                                             CGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTC                                                 >  SRR4302540.1979575/1‑94 (MQ=60)

                                             CGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTC                                                 <  SRR4302540.2580594/94‑1 (MQ=60)

                                              GTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCT                                                >  SRR4302540.2071330/1‑94 (MQ=60)

                                              GTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCT                                                >  SRR4302540.4125781/1‑94 (MQ=60)

                                               TAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTC                                               <  SRR4302540.3021579/94‑1 (MQ=60)

                                               TAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTC                                               >  SRR4302540.2874558/1‑94 (MQ=60)

                                                 AATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGA                                             >  SRR4302540.7240692/1‑94 (MQ=60)

                                                 AATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGA                                             >  SRR4302540.2869701/1‑94 (MQ=60)

                                                 AATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGA                                             >  SRR4302540.5186826/1‑94 (MQ=60)

                                                    TAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTT                                          <  SRR4302540.8246620/94‑1 (MQ=60)

                                                    TAATGGCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTT                                          <  SRR4302540.8655452/94‑1 (MQ=60)

                                                     AATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTG                                         <  SRR4302540.9217235/94‑1 (MQ=60)

                                                     AATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTG                                         >  SRR4302540.9321286/1‑94 (MQ=60)

                                                      ATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGG                                        <  SRR4302540.6262383/94‑1 (MQ=60)

                                                      ATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGG                                        >  SRR4302540.1749695/1‑94 (MQ=60)

                                                       TGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGC                                       >  SRR4302540.4717176/1‑94 (MQ=60)

                                                       TGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGC                                       <  SRR4302540.5753442/94‑1 (MQ=60)

                                                         TCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGG                                     >  SRR4302540.6504540/1‑94 (MQ=60)

                                                          CTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGA                                    >  SRR4302540.6311543/1‑94 (MQ=60)

                                                          CTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGA                                    >  SRR4302540.7583201/1‑94 (MQ=60)

                                                           TGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGAT                                   >  SRR4302540.1703854/1‑94 (MQ=60)

                                                            GCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATA                                  >  SRR4302540.8110346/1‑94 (MQ=60)

                                                            GCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATA                                  >  SRR4302540.8942268/1‑94 (MQ=60)

                                                              TCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACA                                <  SRR4302540.2781716/94‑1 (MQ=60)

                                                              CCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACA                                <  SRR4302540.8293544/94‑1 (MQ=60)

                                                               CTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAG                               >  SRR4302540.5530738/1‑94 (MQ=60)

                                                               CTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAG                               >  SRR4302540.7887149/1‑94 (MQ=60)

                                                                GCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGA                              <  SRR4302540.3390049/94‑1 (MQ=60)

                                                                 tctCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGAT                             <  SRR4302540.8156460/91‑1 (MQ=60)

                                                                 CCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGAT                             <  SRR4302540.6745835/94‑1 (MQ=60)

                                                                 CCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGAT                             >  SRR4302540.3405935/1‑94 (MQ=60)

                                                                  CCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATA                            <  SRR4302540.8226376/94‑1 (MQ=60)

                                                                  CCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATA                            >  SRR4302540.4736121/1‑94 (MQ=60)

                                                                  CCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATA                            >  SRR4302540.4018878/1‑94 (MQ=60)

                                                                   CCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGTTACAGATAT                           >  SRR4302540.9150768/1‑94 (MQ=60)

                                                                   CCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATAT                           >  SRR4302540.1204919/1‑94 (MQ=60)

                                                                   CCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATAT                           <  SRR4302540.7373612/94‑1 (MQ=60)

                                                                   CCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATAT                           <  SRR4302540.7373611/94‑1 (MQ=60)

                                                                   CCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATAT                           >  SRR4302540.192196/1‑94 (MQ=60)

                                                                    CGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATC                          <  SRR4302540.138382/94‑1 (MQ=60)

                                                                    CGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATC                          >  SRR4302540.9059568/1‑94 (MQ=60)

                                                                     tgtgatgggcgtgtGGGGATTGGACTGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCC                         <  SRR4302540.7973831/80‑1 (MQ=60)

                                                                     GTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCC                         >  SRR4302540.3726796/1‑94 (MQ=60)

                                                                     GTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCC                         <  SRR4302540.7904700/94‑1 (MQ=60)

                                                                     GTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCC                         >  SRR4302540.3726795/1‑94 (MQ=60)

                                                                      TGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCG                        <  SRR4302540.6429457/94‑1 (MQ=60)

                                                                      TGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCG                        >  SRR4302540.4667352/1‑94 (MQ=60)

                                                                        GATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGT                      >  SRR4302540.4864274/1‑94 (MQ=60)

                                                                         ATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTT                     <  SRR4302540.7077151/94‑1 (MQ=60)

                                                                         ATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTT                     <  SRR4302540.2600657/94‑1 (MQ=60)

                                                                         ATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTT                     <  SRR4302540.3037843/94‑1 (MQ=60)

                                                                           GACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTA                   <  SRR4302540.6720249/94‑1 (MQ=60)

                                                                              GTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCC                <  SRR4302540.1400326/94‑1 (MQ=60)

                                                                              GTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCC                <  SRR4302540.2134778/94‑1 (MQ=60)

                                                                              GTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCC                >  SRR4302540.9596216/1‑94 (MQ=60)

                                                                              GTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCC                >  SRR4302540.6688545/1‑94 (MQ=60)

                                                                              GTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCC                <  SRR4302540.7323263/94‑1 (MQ=60)

                                                                               TCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCG               >  SRR4302540.4282781/1‑94 (MQ=60)

                                                                               GCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCG               <  SRR4302540.2520896/94‑1 (MQ=60)

                                                                                CTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATCCAGATATCCGGTTTATCTGT              >  SRR4302540.7606017/1‑94 (MQ=60)

                                                                                CTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGT              >  SRR4302540.8883561/1‑94 (MQ=60)

                                                                                CTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGT              <  SRR4302540.8099105/94‑1 (MQ=60)

                                                                                  GGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAA            >  SRR4302540.7649542/1‑94 (MQ=60)

                                                                                  GGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAA            <  SRR4302540.4680486/94‑1 (MQ=60)

                                                                                   GGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAA           <  SRR4302540.9372620/94‑1 (MQ=60)

                                                                                   GGGGATTGGGAGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAA           >  SRR4302540.6177947/1‑94 (MQ=60)

                                                                                     GGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACA         >  SRR4302540.433899/1‑94 (MQ=60)

                                                                                      GATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACAT        >  SRR4302540.9059380/1‑94 (MQ=60)

                                                                                       ATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAATCCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATT       <  SRR4302540.2306773/94‑1 (MQ=60)

                                                                                        TTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTT      <  SRR4302540.4159150/94‑1 (MQ=60)

                                                                                        TTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTT      <  SRR4302540.5660320/94‑1 (MQ=60)

                                                                                         TGGGCGGCGGATCAGCAAACAACTGGACGCGATGGGGATCAAAACCGGTCTCGCTTTGGCTCATACAGATATCCGGTTTATCCGTACACATTTT     >  SRR4302540.9799725/1‑94 (MQ=60)

                                                                                         TGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATGCAGATATCCGGTTTATCCGTAAACATTTT     >  SRR4302540.8917934/1‑94 (MQ=60)

                                                                                         TGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATGCAGATATCCGGTTTATCCGTAAACATTTT     >  SRR4302540.8917933/1‑94 (MQ=60)

                                                                                         TGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTTT     <  SRR4302540.143175/94‑1 (MQ=60)

                                                                                         TGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTTT     <  SRR4302540.220515/94‑1 (MQ=60)

                                                                                          GGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTTTA    <  SRR4302540.9266578/94‑1 (MQ=60)

                                                                                          GGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTTTA    <  SRR4302540.9126482/94‑1 (MQ=60)

                                                                                           GGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTTTAA   <  SRR4302540.3150441/94‑1 (MQ=60)

                                                                                           GGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTTTAA   >  SRR4302540.4632454/1‑94 (MQ=60)

                                                                                            GCGGCGGATCAGCAAAAAACTGGATGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTTTAAT  <  SRR4302540.9415352/94‑1 (MQ=60)

                                                                                            GCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGTATACAGATATCCGGTTTATCCGTAAACATTTTAAT  <  SRR4302540.801700/94‑1 (MQ=60)

                                                                                            GCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTTTAAT  <  SRR4302540.801699/94‑1 (MQ=60)

CGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTTTAAT  >  NC_000913/1230880‑1231065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`