Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,233,687 A→G V85A (GTC→GCC)  nhaB ← Na+/H+ antiporter, pH independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,233,6870AG100.0% 633.0 / NA 198V85A (GTC→GCC) nhaBNa+/H+ antiporter, pH independent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (115/83);  total (115/83)

Only 100 of 206 total aligned reads displayed.

AACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCC  >  NC_000913/1233597‑1233776
                                                                                          |                                                                                         
   agcaacagcTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCgtgt                                                                                      <  1:5621548/93‑1 (MQ=255)
   agcaacagcTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCgtgt                                                                                      <  2:1349658/93‑1 (MQ=255)
   agcaacagcTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCgtgt                                                                                      <  2:1545774/93‑1 (MQ=255)
     caacagcTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTc                                                                                    <  1:1091823/93‑1 (MQ=255)
     caacagcTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTc                                                                                    <  1:5320609/93‑1 (MQ=255)
     caacagcTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTc                                                                                    <  2:3570288/93‑1 (MQ=255)
       acagcTGTTGCATAAAATAGATCCCCGCAACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCcg                                                                                  <  1:33052/93‑1 (MQ=255)
        cagcTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCcgc                                                                                 >  2:5638545/1‑93 (MQ=255)
         agcTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCcgcg                                                                                >  1:4846122/1‑93 (MQ=255)
         agcTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCcgcg                                                                                >  2:4065791/1‑93 (MQ=255)
          gcTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCgcgc                                                                               >  2:4899549/1‑93 (MQ=255)
           cTGTTTCATAAAATAGATACCCGACACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCt                                                                              >  2:2427316/1‑93 (MQ=255)
             gTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTgg                                                                            <  2:665719/93‑1 (MQ=255)
              tttCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGt                                                                           >  1:2568530/1‑93 (MQ=255)
                 cATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCAt                                                                        >  1:4673982/1‑93 (MQ=255)
                  aTAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATg                                                                       >  1:185059/1‑93 (MQ=255)
                     aaaTAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCg                                                                    >  2:3358396/1‑93 (MQ=255)
                     aaaTAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCg                                                                    >  1:1772540/1‑93 (MQ=255)
                       aTAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGAt                                                                  >  1:3689903/1‑93 (MQ=255)
                        tAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATg                                                                 <  1:2790823/93‑1 (MQ=255)
                        tAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATg                                                                 >  1:3276060/1‑93 (MQ=255)
                        tAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATg                                                                 >  1:1215944/1‑93 (MQ=255)
                         aGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGa                                                                <  1:1046083/93‑1 (MQ=255)
                          gATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGaa                                                               >  2:911825/1‑93 (MQ=255)
                          gATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGaa                                                               >  1:3101626/1‑93 (MQ=255)
                           aTACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAAt                                                              >  2:1770410/1‑93 (MQ=255)
                            tACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATa                                                             >  2:3815041/1‑93 (MQ=255)
                             aCCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGACTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATAc                                                            <  1:4590496/93‑1 (MQ=255)
                              cccGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATAcc                                                           <  2:244107/93‑1 (MQ=255)
                                cGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGc                                                         <  2:5022812/93‑1 (MQ=255)
                                cGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGc                                                         >  1:1692034/1‑93 (MQ=255)
                                  ccaccaTAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCtt                                                       >  1:3999224/1‑93 (MQ=255)
                                    accaTAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCg                                                     <  2:4956214/93‑1 (MQ=255)
                                     ccaTAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGa                                                    >  1:671608/1‑93 (MQ=255)
                                      caTAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGAt                                                   <  2:1912270/93‑1 (MQ=255)
                                       aTAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATa                                                  <  1:3626482/93‑1 (MQ=255)
                                       aTAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATa                                                  <  2:4514144/93‑1 (MQ=255)
                                        tAAACATCAGTAACAGCAAGACTTCAAGATTTGTCGCCAACTCTTCATGGGCGTGTTCCGAGCTGGTCATGCCGATGAATACCGCTTCGATAg                                                 >  2:5535265/1‑93 (MQ=255)
                                        tAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAg                                                 >  2:2409064/1‑93 (MQ=255)
                                         aaaCATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGc                                                >  1:897804/1‑93 (MQ=255)
                                          aaCATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGcc                                               >  1:3204/1‑93 (MQ=255)
                                           aCATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCa                                              <  2:5303855/93‑1 (MQ=255)
                                            cATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCaa                                             >  2:5401679/1‑93 (MQ=255)
                                            cATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCaa                                             >  1:1461405/1‑93 (MQ=255)
                                             aTCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAAc                                            <  2:4074491/93‑1 (MQ=255)
                                             aTCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAAc                                            >  2:5255825/1‑93 (MQ=255)
                                             aTCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAAc                                            >  2:6222043/1‑93 (MQ=255)
                                              tCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACa                                           >  1:2005457/1‑93 (MQ=255)
                                              tCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACa                                           <  2:3946194/93‑1 (MQ=255)
                                               cAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAg                                          >  2:4002963/1‑93 (MQ=255)
                                                 gTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGac                                        >  1:522543/1‑93 (MQ=255)
                                                   aaCAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGacca                                      >  1:5682946/1‑93 (MQ=255)
                                                    aCAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGaccac                                     <  2:4674841/93‑1 (MQ=255)
                                                    aCAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGaccac                                     >  1:535363/1‑93 (MQ=255)
                                                    aCAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGaccac                                     >  2:4267818/1‑93 (MQ=255)
                                                    aCAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGaccac                                     <  2:1198918/93‑1 (MQ=255)
                                                     cAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGaccacc                                    <  2:5797456/93‑1 (MQ=255)
                                                      aGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCg                                   <  1:4853268/93‑1 (MQ=255)
                                                      aGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCg                                   <  1:2229468/93‑1 (MQ=255)
                                                       gCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCgg                                  >  1:311024/1‑93 (MQ=255)
                                                       gCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCgg                                  >  1:326478/1‑93 (MQ=255)
                                                        cAAGCCTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACAGCTTCGATAGCCAACAGACCACCggg                                 >  2:4148484/1‑93 (MQ=255)
                                                        cAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCggg                                 >  1:5101431/1‑93 (MQ=255)
                                                        cAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCggg                                 <  2:1288114/93‑1 (MQ=255)
                                                         aaGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCgggg                                >  1:4364822/1‑93 (MQ=255)
                                                         aaGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCgggg                                >  1:5175681/1‑93 (MQ=255)
                                                          aGATTTCAAGATTTGCGGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGa                               <  1:5784166/93‑1 (MQ=255)
                                                          aGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGa                               <  2:1084390/93‑1 (MQ=255)
                                                          aGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGa                               <  1:1303326/93‑1 (MQ=255)
                                                          aGACTTCAAGAGTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGa                               <  2:6082503/93‑1 (MQ=255)
                                                           gACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGag                              <  2:3284425/93‑1 (MQ=255)
                                                            aCTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGagc                             <  1:655411/93‑1 (MQ=255)
                                                             cTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGagca                            >  2:3127304/1‑93 (MQ=255)
                                                               tCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGagcagc                          >  2:4309743/1‑93 (MQ=255)
                                                               tCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGagcagc                          <  1:2357358/93‑1 (MQ=255)
                                                               tCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGagcagc                          >  1:1251810/1‑93 (MQ=255)
                                                               tCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGagcagc                          >  2:2673489/1‑93 (MQ=255)
                                                                cAAGATTTACCGCCACCTCTTCACGGGCGCTGTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCg                         <  2:2992013/93‑1 (MQ=255)
                                                                 aaGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCgg                        <  2:3891065/93‑1 (MQ=255)
                                                                 aaGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCgg                        >  1:3541387/1‑93 (MQ=255)
                                                                 aaGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCgg                        <  2:5138729/93‑1 (MQ=255)
                                                                  aGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCggg                       <  1:2447800/93‑1 (MQ=255)
                                                                   gATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGt                      <  2:5434993/93‑1 (MQ=255)
                                                                    aTTTGCCGCCCCCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTa                     <  1:4305263/93‑1 (MQ=255)
                                                                     tttGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGGAg                    >  1:3407087/1‑93 (MQ=255)
                                                                        gccgccACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCAt                 >  2:3776743/1‑93 (MQ=255)
                                                                         ccgccACCTCTTCACGGGCGTGTACCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCAtt                <  1:777907/93‑1 (MQ=255)
                                                                          cgccACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCAttt               >  2:5491301/1‑93 (MQ=255)
                                                                           gccACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTc              <  1:1585953/93‑1 (MQ=255)
                                                                           gccACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTc              >  1:4492632/1‑93 (MQ=255)
                                                                           gccACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGGAGCATTTc              >  1:5398878/1‑93 (MQ=255)
                                                                            ccACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCa             >  1:140518/1‑93 (MQ=255)
                                                                             cACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAg            >  2:2068123/1‑93 (MQ=255)
                                                                              cccTCTTCGCGGGCGTGTTCCGCGCTGTTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAgg           <  1:3436702/92‑1 (MQ=255)
                                                                              aCCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAgg           >  1:4140812/1‑93 (MQ=255)
                                                                                 tctTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGcc        >  1:3486881/1‑93 (MQ=255)
                                                                                  ctTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCa       >  1:5986919/1‑93 (MQ=255)
                                                                                  ctTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCa       <  1:5850763/93‑1 (MQ=255)
                                                                                       cGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGcc  <  2:2141562/93‑1 (MQ=255)
                                                                                       cGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGGAGCATTTCAGGGCCATCGcc  >  1:4205507/1‑93 (MQ=255)
                                                                                          |                                                                                         
AACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCC  >  NC_000913/1233597‑1233776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAGAG  >  NC_000913/1233594‑1233780
                                                                                             |                                                                                             
ATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGG                                                                                               <  SRR4302533.5152475/94‑1 (MQ=60)
ATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGG                                                                                               <  SRR4302533.664192/94‑1 (MQ=60)
 TGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGC                                                                                              <  SRR4302533.6062805/94‑1 (MQ=60)
  GAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCG                                                                                             <  SRR4302533.928605/94‑1 (MQ=60)
   AACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGT                                                                                            <  SRR4302533.717642/94‑1 (MQ=60)
      AGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTT                                                                                         <  SRR4302533.5655529/94‑1 (MQ=60)
      AGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTT                                                                                         <  SRR4302533.2275202/94‑1 (MQ=60)
      AGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTT                                                                                         <  SRR4302533.5561810/94‑1 (MQ=60)
        CAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCC                                                                                       <  SRR4302533.1098279/94‑1 (MQ=60)
        CAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCC                                                                                       <  SRR4302533.5352495/94‑1 (MQ=60)
          ACAGCTGTTTAATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGA                                                                                     <  SRR4302533.672220/94‑1 (MQ=60)
          ACAGCTGTTGCATAAAATAGATCCCCGCAACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGC                                                                                     <  SRR4302533.33275/94‑1 (MQ=60)
           CAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCG                                                                                    >  SRR4302533.3121725/1‑94 (MQ=60)
           CAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCG                                                                                    >  SRR4302533.4875491/1‑94 (MQ=60)
           AAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCCCCTCTTCACGGGCGTGTTCCGCG                                                                                    <  SRR4302533.1308923/94‑1 (MQ=60)
            AGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGC                                                                                   >  SRR4302533.3348267/1‑94 (MQ=60)
            AGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGC                                                                                   >  SRR4302533.4522845/1‑94 (MQ=60)
              CTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTG                                                                                 >  SRR4302533.4183714/1‑94 (MQ=60)
              CTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTG                                                                                 >  SRR4302533.449902/1‑94 (MQ=60)
                GTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGT                                                                               >  SRR4302533.2584335/1‑94 (MQ=60)
                GTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGT                                                                               <  SRR4302533.1416743/94‑1 (MQ=60)
                GTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGT                                                                               <  SRR4302533.6214120/94‑1 (MQ=60)
                  TTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCA                                                                             >  SRR4302533.5203816/1‑94 (MQ=60)
                   TCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCAT                                                                            >  SRR4302533.4702302/1‑94 (MQ=60)
                   TCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCAT                                                                            >  SRR4302533.1235971/1‑94 (MQ=60)
                    CATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATG                                                                           >  SRR4302533.186208/1‑94 (MQ=60)
                    CATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATG                                                                           <  SRR4302533.3686866/94‑1 (MQ=60)
                     ATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGC                                                                          >  SRR4302533.4909628/1‑94 (MQ=60)
                      TAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCC                                                                         <  SRR4302533.2993289/94‑1 (MQ=60)
                      TAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCC                                                                         <  SRR4302533.1501617/94‑1 (MQ=60)
                       AAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCG                                                                        >  SRR4302533.1783334/1‑94 (MQ=60)
                         AATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGAT                                                                      >  SRR4302533.3712572/1‑94 (MQ=60)
                          ATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATG                                                                     <  SRR4302533.531306/94‑1 (MQ=60)
                          ATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATG                                                                     >  SRR4302533.1223249/1‑94 (MQ=60)
                          ATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATG                                                                     >  SRR4302533.3296288/1‑94 (MQ=60)
                           TAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGA                                                                    <  SRR4302533.2807987/94‑1 (MQ=60)
                            AGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAA                                                                   >  SRR4302533.3120693/1‑94 (MQ=60)
                            AGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAA                                                                   <  SRR4302533.1052231/94‑1 (MQ=60)
                            AGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAA                                                                   >  SRR4302533.3480374/1‑94 (MQ=60)
                                ACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACC                                                               >  SRR4302533.322783/1‑94 (MQ=60)
                                ACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGACTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACC                                                               <  SRR4302533.4618301/94‑1 (MQ=60)
                                 CCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCG                                                              >  SRR4302533.1713497/1‑94 (MQ=60)
                                 CCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCG                                                              <  SRR4302533.2495206/94‑1 (MQ=60)
                                  CCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGC                                                             >  SRR4302533.1702282/1‑94 (MQ=60)
                                   CGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCT                                                            >  SRR4302533.3287818/1‑94 (MQ=60)
                                    GCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTT                                                           >  SRR4302533.4023574/1‑94 (MQ=60)
                                    GCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTT                                                           >  SRR4302533.899226/1‑94 (MQ=60)
                                     CCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTC                                                          >  SRR4302533.2012186/1‑94 (MQ=60)
                                      CACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCG                                                         <  SRR4302533.392960/94‑1 (MQ=60)
                                       ACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGA                                                        <  SRR4302533.538518/94‑1 (MQ=60)
                                       ACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGA                                                        >  SRR4302533.675574/1‑94 (MQ=60)
                                        CCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGAT                                                       >  SRR4302533.3155293/1‑94 (MQ=60)
                                          ATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAG                                                     >  SRR4302533.1334276/1‑94 (MQ=60)
                                          ATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAG                                                     <  SRR4302533.3648794/94‑1 (MQ=60)
                                           TAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGC                                                    >  SRR4302533.903074/1‑94 (MQ=60)
                                            AAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCC                                                   >  SRR4302533.1159972/1‑94 (MQ=60)
                                            AAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCC                                                   >  SRR4302533.3229/1‑94 (MQ=60)
                                              ACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAA                                                 >  SRR4302533.1470214/1‑94 (MQ=60)
                                               CATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAAC                                                >  SRR4302533.2420402/1‑94 (MQ=60)
                                                ATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACA                                               >  SRR4302533.2017807/1‑94 (MQ=60)
                                                  CAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGA                                             >  SRR4302533.343645/1‑94 (MQ=60)
                                                   AGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGAC                                            >  SRR4302533.525643/1‑94 (MQ=60)
                                                    GTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACC                                           <  SRR4302533.4442968/94‑1 (MQ=60)
                                                     TAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCA                                          <  SRR4302533.5386056/94‑1 (MQ=60)
                                                     TAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCA                                          >  SRR4302533.5717276/1‑94 (MQ=60)
                                                     TAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGCTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCA                                          <  SRR4302533.361493/94‑1 (MQ=60)
                                                      AACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCAC                                         >  SRR4302533.538544/1‑94 (MQ=60)
                                                        CAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCG                                       <  SRR4302533.2798232/94‑1 (MQ=60)
                                                         AGCAAGACTTCAAGATTTGCCGCCCCCTCTTCACGGGCTTGTTCCGCGCTGGTCATGCCGATGAATCCCGCTTCGATAGCCAACAGCCCCCCGG                                      <  SRR4302533.4882512/94‑1 (MQ=60)
                                                         AGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGG                                      <  SRR4302533.2243230/94‑1 (MQ=60)
                                                         AGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGG                                      <  SRR4302533.4882679/94‑1 (MQ=60)
                                                         AGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGG                                      >  SRR4302533.312927/1‑94 (MQ=60)
                                                         AGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGG                                      >  SRR4302533.2041442/1‑94 (MQ=60)
                                                         AGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGG                                      >  SRR4302533.328464/1‑94 (MQ=60)
                                                          GCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGG                                     >  SRR4302533.4281476/1‑94 (MQ=60)
                                                          GCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGG                                     >  SRR4302533.4722337/1‑94 (MQ=60)
                                                          GCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGG                                     >  SRR4302533.5132245/1‑94 (MQ=60)
                                                           CAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGG                                    >  SRR4302533.5206942/1‑94 (MQ=60)
                                                           CAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGG                                    >  SRR4302533.4391173/1‑94 (MQ=60)
                                                           CAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGG                                    >  SRR4302533.5701007/1‑94 (MQ=60)
                                                           CAAGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGG                                    <  SRR4302533.6241178/94‑1 (MQ=60)
                                                             AGATTTCAAGATTTGCGGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAG                                  <  SRR4302533.5819098/94‑1 (MQ=60)
                                                             AGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAG                                  <  SRR4302533.1311158/94‑1 (MQ=60)
                                                             AGACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAG                                  <  SRR4302533.4623243/94‑1 (MQ=60)
                                                              GACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGC                                 <  SRR4302533.1832088/94‑1 (MQ=60)
                                                               ACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCA                                <  SRR4302533.659279/94‑1 (MQ=60)
                                                               ACTTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCA                                >  SRR4302533.4065592/1‑94 (MQ=60)
                                                                 TTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGC                              >  SRR4302533.1259289/1‑94 (MQ=60)
                                                                 TTCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGC                              <  SRR4302533.3558235/94‑1 (MQ=60)
                                                                  TCAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCG                             <  SRR4302533.2371929/94‑1 (MQ=60)
                                                                   CAAGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGG                            >  SRR4302533.3563208/1‑94 (MQ=60)
                                                                     AGATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGT                          <  SRR4302533.2462904/94‑1 (MQ=60)
                                                                       ATTTGCCGCCCCCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAG                        <  SRR4302533.4331167/94‑1 (MQ=60)
                                                                       ATTTGCCGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGGAG                        >  SRR4302533.3428130/1‑94 (MQ=60)
                                                                          TGCCGCCACCTCTTCACGGGCGTGCTCCGCGCTGGTCATGCCGCTGAATACCGCTTCGATAGCCAATAGTCCACCGAGGAGCAGCGGGGAGCAT                     >  SRR4302533.4885268/1‑94 (MQ=60)
                                                                            CCGCCACCTCTTCACGGGCGTGTACCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTT                   <  SRR4302533.782485/94‑1 (MQ=60)
                                                                             CGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTC                  >  SRR4302533.434493/1‑94 (MQ=60)
                                                                             CGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTC                  >  SRR4302533.4519840/1‑94 (MQ=60)
                                                                             CGCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGGAGCATTTC                  >  SRR4302533.5431321/1‑94 (MQ=60)
                                                                              GCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCA                 <  SRR4302533.1595508/94‑1 (MQ=60)
                                                                              GCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCA                 >  SRR4302533.141414/1‑94 (MQ=60)
                                                                              GCCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCA                 >  SRR4302533.5211234/1‑94 (MQ=60)
                                                                               CCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAG                >  SRR4302533.1078640/1‑94 (MQ=60)
                                                                               CCACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGGAGCATTTCAG                >  SRR4302533.287000/1‑94 (MQ=60)
                                                                                CACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGG               >  SRR4302533.3312636/1‑94 (MQ=60)
                                                                                CACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGG               >  SRR4302533.4165925/1‑94 (MQ=60)
                                                                                CACCTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGG               <  SRR4302533.1640234/94‑1 (MQ=60)
                                                                                 CCCTCTTCGCGGGCGTGTTCCGCGCTGTTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGG              <  SRR4302533.3457932/94‑1 (MQ=60)
                                                                                   CTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCC            <  SRR4302533.5805741/94‑1 (MQ=60)
                                                                                   CTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCC            >  SRR4302533.3508383/1‑94 (MQ=60)
                                                                                   CTCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCC            >  SRR4302533.5829800/1‑94 (MQ=60)
                                                                                    TCTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCA           >  SRR4302533.6023176/1‑94 (MQ=60)
                                                                                     CTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCAT          >  SRR4302533.5934813/1‑94 (MQ=60)
                                                                                     CTTCACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCAT          <  SRR4302533.5886136/94‑1 (MQ=60)
                                                                                         ACGGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGGAGCATTTCAGGGCCATCGCC      >  SRR4302533.4230947/1‑94 (MQ=60)
                                                                                           GGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAG    <  SRR4302533.3437464/94‑1 (MQ=60)
                                                                                           GGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAG    <  SRR4302533.2638564/94‑1 (MQ=60)
                                                                                           GGGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAG    >  SRR4302533.5063267/1‑94 (MQ=60)
                                                                                            GGCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAGA   <  SRR4302533.466744/94‑1 (MQ=60)
                                                                                             GCGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAGAG  <  SRR4302533.5827843/94‑1 (MQ=60)
                                                                                             GCGTGTTCAGCGCTGCTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAGAG  <  SRR4302533.3588056/94‑1 (MQ=60)
                                                                                             |                                                                                             
ATGAACAGCAACAGCTGTTTCATAAAATAGATACCCGCCACCATAAACATCAGTAACAGCAAGACTTCAAGATTTGCCGCCACCTCTTCACGGACGTGTTCCGCGCTGGTCATGCCGATGAATACCGCTTCGATAGCCAACAGACCACCGGGGAGCAGCGGGTAGCATTTCAGGGCCATCGCCAGAG  >  NC_000913/1233594‑1233780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑