Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,233,892 A→G S17P (TCC→CCC)  nhaB ← Na+/H+ antiporter, pH independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,233,8920AG100.0% 812.0 / NA 265S17P (TCC→CCC) nhaBNa+/H+ antiporter, pH independent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (153/112);  total (153/112)

Only 100 of 279 total aligned reads displayed.

GCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTA  >  NC_000913/1233802‑1233981
                                                                                          |                                                                                         
   aCCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGc                                                                                      >  2:3694863/1‑93 (MQ=255)
   aCCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGc                                                                                      >  2:5773807/1‑93 (MQ=255)
    ccAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGcc                                                                                     >  2:3105282/1‑93 (MQ=255)
       gCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCaa                                                                                  >  1:1785140/1‑93 (MQ=255)
       gCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCaa                                                                                  >  1:7001132/1‑93 (MQ=255)
        cccGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCaaa                                                                                 <  1:1184185/93‑1 (MQ=255)
         ccGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCaaaa                                                                                >  2:1356016/1‑93 (MQ=255)
           gcgACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCaaaaaa                                                                              >  2:3573013/1‑93 (MQ=255)
           gcgACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCaaaaaa                                                                              >  1:6139108/1‑93 (MQ=255)
           gcgACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCaaaaaa                                                                              >  2:9639253/1‑93 (MQ=255)
            cgacgaAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAAt                                                                             <  1:249520/93‑1 (MQ=255)
            cgacgaAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAAt                                                                             <  2:53906/93‑1 (MQ=255)
             gacgaAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAAtt                                                                            >  1:5283586/1‑93 (MQ=255)
              acgaAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTg                                                                           >  2:4878666/1‑93 (MQ=255)
              acgaAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTg                                                                           >  2:2991968/1‑93 (MQ=255)
               cgaAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTgc                                                                          >  2:7947046/1‑93 (MQ=255)
               cgaAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTgc                                                                          >  1:6009300/1‑93 (MQ=255)
                gaAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTgcg                                                                         >  1:1575852/1‑93 (MQ=255)
                  aaGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGcc                                                                       <  1:8273635/93‑1 (MQ=255)
                     ggCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATa                                                                    <  2:4081121/93‑1 (MQ=255)
                      gCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAg                                                                   <  1:427498/93‑1 (MQ=255)
                       gtgatgaGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAgc                                                                  <  2:7394016/92‑1 (MQ=255)
                        tgatgaGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAgcg                                                                 >  1:3369333/1‑93 (MQ=255)
                          atgaGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAgcgcg                                                               >  2:978256/1‑93 (MQ=255)
                          atgaGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAgcgcg                                                               >  2:63621/1‑93 (MQ=255)
                           tgtggAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAACTTGCGCCATAgcgcgc                                                              >  1:6660222/4‑93 (MQ=255)
                            gaGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGcgcgcg                                                             <  1:6329574/93‑1 (MQ=255)
                             aGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCgg                                                            >  1:2833387/1‑93 (MQ=255)
                              ggAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGc                                                           >  1:1656463/1‑93 (MQ=255)
                               gAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGGGGcc                                                          >  1:624367/1‑93 (MQ=255)
                                aaaaTTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGccc                                                         <  2:2493951/93‑1 (MQ=255)
                                aaaaTTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGccc                                                         <  1:2142465/93‑1 (MQ=255)
                                   aTTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAg                                                      <  2:4156198/93‑1 (MQ=255)
                                   aTTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAg                                                      >  1:8884567/1‑93 (MQ=255)
                                   aTTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAg                                                      >  2:1724705/1‑93 (MQ=255)
                                   aTTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAg                                                      <  1:4546226/93‑1 (MQ=255)
                                   aTTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAg                                                      >  2:824929/1‑93 (MQ=255)
                                    atAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAgg                                                     <  1:3582053/92‑1 (MQ=255)
                                     tAACGGGTTTACGATTAAGAAAATGATGGGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGga                                                    <  2:7131599/93‑1 (MQ=255)
                                      aaCGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGgag                                                   >  1:5527888/1‑93 (MQ=255)
                                          ggTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGAtct                                               >  1:1707844/1‑93 (MQ=255)
                                          ggTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGAtct                                               <  2:6220090/93‑1 (MQ=255)
                                           gTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGAtctc                                              <  2:4507103/93‑1 (MQ=255)
                                            tttACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTcc                                             >  1:4075143/1‑93 (MQ=255)
                                            tttACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTcc                                             <  2:6353261/93‑1 (MQ=255)
                                             ttACGATTAAGAAAACGATGAGGGCGACTGTGTACCAGGCGGGGGGCTGTCCCAAAAAATTGCGCCATACCGCGCGGCCCCAGGAGATCTCCa                                            <  1:3840084/93‑1 (MQ=255)
                                              tACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCat                                           <  2:2353061/93‑1 (MQ=255)
                                                 gATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCatgat                                        >  2:8616376/1‑93 (MQ=255)
                                                 gATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCatgat                                        >  1:7721376/1‑93 (MQ=255)
                                                 gATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCatgat                                        <  1:4930013/93‑1 (MQ=255)
                                                    tAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGt                                     <  1:2300218/93‑1 (MQ=255)
                                                    tAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGt                                     <  2:3176734/93‑1 (MQ=255)
                                                     aaGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGtt                                    >  1:1369015/1‑93 (MQ=255)
                                                      aGAAACTGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGttt                                   <  2:5167342/93‑1 (MQ=255)
                                                      aGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGttt                                   >  1:420027/1‑93 (MQ=255)
                                                      aGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGttt                                   <  1:5610347/93‑1 (MQ=255)
                                                       gAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTc                                  <  2:90558/93‑1 (MQ=255)
                                                       gAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTc                                  >  1:8219691/1‑93 (MQ=255)
                                                       gAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTc                                  >  2:4405640/1‑93 (MQ=255)
                                                       gAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTc                                  >  1:5957855/1‑93 (MQ=255)
                                                         aaaTGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTccc                                >  1:9456638/1‑93 (MQ=255)
                                                         aaaTGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTccc                                >  1:8037679/1‑93 (MQ=255)
                                                          aaTGATGAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCcct                               <  2:8458946/93‑1 (MQ=255)
                                                           atgatgAGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCcctt                              <  2:9612240/93‑1 (MQ=255)
                                                            tgatgaGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCcctta                             <  1:1066778/93‑1 (MQ=255)
                                                            tgatgaGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCcctta                             <  1:9227891/93‑1 (MQ=255)
                                                            tgatgaGGGCGAGTTTGAACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCcctta                             >  1:6592702/1‑93 (MQ=255)
                                                             gatgaGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCccttac                            <  2:6449533/93‑1 (MQ=255)
                                                               tgaGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCccttacct                          <  2:7711621/93‑1 (MQ=255)
                                                                 aGGGCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCccttacctta                        <  2:1175854/93‑1 (MQ=255)
                                                                   ggCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACa                      <  1:3249962/93‑1 (MQ=255)
                                                                   ggCGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACa                      <  2:34137/93‑1 (MQ=255)
                                                                     cGAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACaaa                    <  2:8380196/93‑1 (MQ=255)
                                                                      gAGTTTGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAaat                   >  2:6782093/1‑93 (MQ=255)
                                                                       attttGTACCAGTCGGGGGGCTGGCCAAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAaata                  <  2:48482/91‑1 (MQ=255)
                                                                         tttGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAaataat                >  1:10047151/1‑93 (MQ=255)
                                                                         tttGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAaataat                >  2:2181920/1‑93 (MQ=255)
                                                                         tttGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAaataat                >  1:5750987/1‑93 (MQ=255)
                                                                         tttGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAaataat                <  1:8485749/93‑1 (MQ=255)
                                                                          ttGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATc               <  1:7772143/93‑1 (MQ=255)
                                                                          ttGTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATc               >  1:7415257/1‑93 (MQ=255)
                                                                            gTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCaa             <  1:2681486/93‑1 (MQ=255)
                                                                            gTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCaa             <  2:8512630/93‑1 (MQ=255)
                                                                            gTACCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCaa             <  1:978256/93‑1 (MQ=255)
                                                                              aCCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAatg           >  1:8611163/1‑93 (MQ=255)
                                                                              aCCAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAatg           <  2:9195454/93‑1 (MQ=255)
                                                                               ccAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAatga          >  1:5890988/1‑93 (MQ=255)
                                                                               ccAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAatga          >  2:4569780/1‑93 (MQ=255)
                                                                                cAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAatgat         >  2:8529372/1‑93 (MQ=255)
                                                                                cAGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAatgat         <  2:1511033/93‑1 (MQ=255)
                                                                                 aGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAatgatg        >  1:7189425/1‑93 (MQ=255)
                                                                                 aGTCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAatgatg        <  2:5507538/93‑1 (MQ=255)
                                                                                   tCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTGCCTTACCTTGCAAATAATGAatgatggt      >  2:1962730/1‑91 (MQ=255)
                                                                                   tCGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGtt      >  2:920798/1‑93 (MQ=255)
                                                                                    cGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGttt     <  2:7910403/93‑1 (MQ=255)
                                                                                      ggggGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGttttt   >  1:788976/1‑93 (MQ=255)
                                                                                      ggggGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGttttt   >  2:7565078/1‑93 (MQ=255)
                                                                                      ggggGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGttttt   <  1:896815/93‑1 (MQ=255)
                                                                                       ggggCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTa  <  1:2984969/93‑1 (MQ=255)
                                                                                       ggggCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTa  <  2:2529407/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTA  >  NC_000913/1233802‑1233981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTATG  >  NC_000913/1233799‑1233983
                                                                                              |                                                                                           
CCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGG                                                                                             <  SRR4302540.2521158/94‑1 (MQ=60)
 CAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGC                                                                                            >  SRR4302540.4529793/1‑94 (MQ=60)
   GCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTG                                                                                          <  SRR4302540.9483275/94‑1 (MQ=60)
     AACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGC                                                                                        >  SRR4302540.8762934/1‑94 (MQ=60)
     AACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGATTACGATTAAGAAAATGATGACGGCGTGTTGGTACCCGTC‑GGGGGGCTGGC                                                                                        >  SRR4302540.3140991/1‑94 (MQ=60)
      ACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCC                                                                                       >  SRR4302540.6727677/1‑94 (MQ=60)
        CAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCA                                                                                     >  SRR4302540.4323734/1‑94 (MQ=60)
         AGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAA                                                                                    >  SRR4302540.7078819/1‑94 (MQ=60)
         AGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAA                                                                                    >  SRR4302540.1318280/1‑94 (MQ=60)
         AGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAA                                                                                    >  SRR4302540.1804529/1‑94 (MQ=60)
          GCCCGCGACGAAAGGGCTGATGAGGAAAATTACCAGGTTTACGATTAAGAAATTGATGCGGGCAAGATTGTACCAGTC‑GGGGGGCCGGCCCACA                                                                                   >  SRR4302540.8391619/1‑94 (MQ=60)
          GCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAA                                                                                   >  SRR4302540.8391618/1‑94 (MQ=60)
           CCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAA                                                                                  <  SRR4302540.1196408/94‑1 (MQ=60)
            CCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGGCTGGCCCAAAA                                                                                  >  SRR4302540.1114478/1‑94 (MQ=60)
            CCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGATGGCCCAAAAA                                                                                 >  SRR4302540.3274221/1‑94 (MQ=60)
             CGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAA                                                                                >  SRR4302540.6207482/1‑94 (MQ=60)
              GCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTCGGGGGGGCTGGCCCAAAAAA                                                                                >  SRR4302540.4897218/1‑94 (MQ=60)
              GCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAAT                                                                               >  SRR4302540.6319243/1‑94 (MQ=60)
               CGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATT                                                                              <  SRR4302540.251937/94‑1 (MQ=60)
               CGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATT                                                                              >  SRR4302540.5341321/1‑94 (MQ=60)
               CGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATT                                                                              >  SRR4302540.5812909/1‑94 (MQ=60)
               CGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATT                                                                              >  SRR4302540.7435864/1‑94 (MQ=60)
                GACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTG                                                                             <  SRR4302540.6308519/94‑1 (MQ=60)
                 ACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGC                                                                            >  SRR4302540.1798046/1‑94 (MQ=60)
                 ACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGC                                                                            >  SRR4302540.6075936/1‑94 (MQ=60)
                  CGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCG                                                                           >  SRR4302540.1593096/1‑94 (MQ=60)
                    AAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCC                                                                         <  SRR4302540.2208299/94‑1 (MQ=60)
                     AAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCA                                                                        <  SRR4302540.3918598/94‑1 (MQ=60)
                     AAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCA                                                                        <  SRR4302540.8369750/94‑1 (MQ=60)
                     AAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCA                                                                        >  SRR4302540.9667719/1‑94 (MQ=60)
                      AGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCAT                                                                       <  SRR4302540.7200730/94‑1 (MQ=60)
                      AGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCAC                                                                       >  SRR4302540.8508562/1‑94 (MQ=60)
                       GGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATA                                                                      <  SRR4302540.417164/94‑1 (MQ=60)
                       GGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATA                                                                      >  SRR4302540.8087324/1‑94 (MQ=60)
                         GCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGC                                                                    <  SRR4302540.431997/94‑1 (MQ=60)
                          CTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCG                                                                   >  SRR4302540.6150618/1‑94 (MQ=60)
                          CTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCG                                                                   >  SRR4302540.3406176/1‑94 (MQ=60)
                          CTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCG                                                                   >  SRR4302540.2104383/1‑94 (MQ=60)
                           TGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGC                                                                  >  SRR4302540.2494317/1‑94 (MQ=60)
                             ATGTGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAACTTGCGCCATAGCGCGC                                                                >  SRR4302540.6733861/1‑94 (MQ=60)
                             ATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGC                                                                >  SRR4302540.1544718/1‑94 (MQ=60)
                              TGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCG                                                               >  SRR4302540.7878343/1‑94 (MQ=60)
                               GAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGG                                                              >  SRR4302540.2864031/1‑94 (MQ=60)
                               GAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGG                                                              <  SRR4302540.6400057/94‑1 (MQ=60)
                               GAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGG                                                              <  SRR4302540.6400058/94‑1 (MQ=60)
                                AGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGC                                                             >  SRR4302540.1674514/1‑94 (MQ=60)
                                 GGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGGGGCC                                                            >  SRR4302540.631011/1‑94 (MQ=60)
                                 GGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCC                                                            >  SRR4302540.1702274/1‑94 (MQ=60)
                                   AAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCC                                                          <  SRR4302540.2165487/94‑1 (MQ=60)
                                   AAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCC                                                          >  SRR4302540.9073695/1‑94 (MQ=60)
                                   AAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCC                                                          >  SRR4302540.6167599/1‑94 (MQ=60)
                                    AAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCA                                                         <  SRR4302540.8589933/94‑1 (MQ=60)
                                     AATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAG                                                        >  SRR4302540.8987407/1‑94 (MQ=60)
                                     AATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAG                                                        >  SRR4302540.8305065/1‑94 (MQ=60)
                                      ATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGG                                                       <  SRR4302540.4595414/94‑1 (MQ=60)
                                      ATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGG                                                       >  SRR4302540.9803160/1‑94 (MQ=60)
                                      ATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGG                                                       <  SRR4302540.8810059/94‑1 (MQ=60)
                                       TTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATTGCGCGCGGCCCCAGGA                                                      >  SRR4302540.9049979/1‑94 (MQ=60)
                                       TTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGA                                                      <  SRR4302540.7555832/94‑1 (MQ=60)
                                       ATAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGA                                                      <  SRR4302540.8640722/94‑1 (MQ=60)
                                       ATAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGA                                                      <  SRR4302540.3621174/94‑1 (MQ=60)
                                        TAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAG                                                     >  SRR4302540.5588770/1‑94 (MQ=60)
                                           CGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATC                                                  <  SRR4302540.2821670/94‑1 (MQ=60)
                                            GGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCT                                                 >  SRR4302540.3735494/1‑94 (MQ=60)
                                            GGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCT                                                 >  SRR4302540.1726300/1‑94 (MQ=60)
                                            GGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCT                                                 >  SRR4302540.1726299/1‑94 (MQ=60)
                                            GGGTTTACGATTAAGAAAATGAGTAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCT                                                 >  SRR4302540.1825732/1‑94 (MQ=60)
                                             GGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTC                                                >  SRR4302540.101723/1‑94 (MQ=60)
                                              GTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCC                                               >  SRR4302540.2214766/1‑94 (MQ=60)
                                              GTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCC                                               >  SRR4302540.4119069/1‑94 (MQ=60)
                                              GTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCC                                               <  SRR4302540.5051727/94‑1 (MQ=60)
                                               TTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCA                                              >  SRR4302540.1104284/1‑94 (MQ=60)
                                               TTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCA                                              >  SRR4302540.4240362/1‑94 (MQ=60)
                                                TTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCAT                                             <  SRR4302540.3881840/94‑1 (MQ=60)
                                                TTACGATTAAGAAAACGATGAGGGCGACTGTGTACCAGGC‑GGGGGGCTGTCCCAAAAAATTGCGCCATACCGCGCGGCCCCAGGAGATCTCCAT                                             <  SRR4302540.3881846/94‑1 (MQ=60)
                                                  ACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGA                                           <  SRR4302540.8282436/94‑1 (MQ=60)
                                                   CGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGAT                                          >  SRR4302540.7811015/1‑94 (MQ=60)
                                                   CGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGAT                                          >  SRR4302540.7811016/1‑94 (MQ=60)
                                                   CGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAAGTGCGCAATAGCGCGCGGCCCCAGGAGATCTCCATGAT                                          >  SRR4302540.1761656/1‑94 (MQ=60)
                                                    GATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATG                                         <  SRR4302540.4983150/94‑1 (MQ=60)
                                                    GATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATG                                         >  SRR4302540.6932148/1‑94 (MQ=60)
                                                     ATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGG                                        >  SRR4302540.7315126/1‑94 (MQ=60)
                                                      TTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGT                                       >  SRR4302540.7948463/1‑94 (MQ=60)
                                                       TAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTT                                      <  SRR4302540.2324959/94‑1 (MQ=60)
                                                       TAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTT                                      >  SRR4302540.1383516/1‑94 (MQ=60)
                                                        AAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTT                                     >  SRR4302540.424438/1‑94 (MQ=60)
                                                         AGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTC                                    >  SRR4302540.6023749/1‑94 (MQ=60)
                                                         AGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTC                                    >  SRR4302540.8315231/1‑94 (MQ=60)
                                                         AGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTC                                    <  SRR4302540.5672066/94‑1 (MQ=60)
                                                         AGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTC                                    >  SRR4302540.6023750/1‑94 (MQ=60)
                                                           AAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCACAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCC                                  >  SRR4302540.5922587/1‑94 (MQ=60)
                                                           AAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCC                                  >  SRR4302540.8131090/1‑94 (MQ=60)
                                                           AAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCC                                  >  SRR4302540.9568858/1‑94 (MQ=60)
                                                           AAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCC                                  >  SRR4302540.5922588/1‑94 (MQ=60)
                                                            AAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCT                                 <  SRR4302540.480710/94‑1 (MQ=60)
                                                             AATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTT                                >  SRR4302540.647997/1‑94 (MQ=60)
                                                             AATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTT                                >  SRR4302540.5749696/1‑94 (MQ=60)
                                                              ATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTA                               <  SRR4302540.6527494/94‑1 (MQ=60)
                                                              ATGATGAGGGCGAGTTTGAACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTA                               >  SRR4302540.6665713/1‑94 (MQ=60)
                                                               TGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTAC                              <  SRR4302540.1077651/94‑1 (MQ=60)
                                                               TGATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTAC                              <  SRR4302540.9338524/94‑1 (MQ=60)
                                                                GATGAGGGCGAGTTTGTACCAGTCGGGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTAC                              <  SRR4302540.4081438/94‑1 (MQ=60)
                                                                GATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACC                             <  SRR4302540.9459388/94‑1 (MQ=60)
                                                                 ATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCT                            <  SRR4302540.5982423/94‑1 (MQ=60)
                                                                 ATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCT                            <  SRR4302540.5982424/94‑1 (MQ=60)
                                                                 ATGAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCT                            >  SRR4302540.3269987/1‑94 (MQ=60)
                                                                   GAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTA                          >  SRR4302540.6208696/1‑94 (MQ=60)
                                                                   GAGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTA                          <  SRR4302540.282681/94‑1 (MQ=60)
                                                                    AGGGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTAACCTCAC                         >  SRR4302540.1734071/1‑94 (MQ=60)
                                                                      GGCGAGTTTGTCCCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAA                       <  SRR4302540.3285363/94‑1 (MQ=60)
                                                                      GGCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAA                       <  SRR4302540.3285362/94‑1 (MQ=60)
                                                                       GCGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAA                      <  SRR4302540.1221994/94‑1 (MQ=60)
                                                                        CGAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAAT                     >  SRR4302540.710682/1‑94 (MQ=60)
                                                                         GAGTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATA                    >  SRR4302540.3552356/1‑94 (MQ=60)
                                                                           GTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGTGATCTCCATGATGGTTTCCCTTACCTTACAAATAAT                  <  SRR4302540.4403670/94‑1 (MQ=60)
                                                                           GTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAAT                  >  SRR4302540.5814462/1‑94 (MQ=60)
                                                                           GTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAAT                  >  SRR4302540.10163945/1‑94 (MQ=60)
                                                                           GTTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAAT                  >  SRR4302540.2952174/1‑94 (MQ=60)
                                                                            TTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATC                 >  SRR4302540.7497852/1‑94 (MQ=60)
                                                                            TTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATC                 <  SRR4302540.8584330/94‑1 (MQ=60)
                                                                            TTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATC                 <  SRR4302540.5264224/94‑1 (MQ=60)
                                                                            TTTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATC                 <  SRR4302540.8355495/94‑1 (MQ=60)
                                                                             TTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCA                <  SRR4302540.7863004/94‑1 (MQ=60)
                                                                             TTGTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCA                <  SRR4302540.4311409/94‑1 (MQ=60)
                                                                               GTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAAT              <  SRR4302540.988287/94‑1 (MQ=60)
                                                                               GTACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAAT              <  SRR4302540.2710484/94‑1 (MQ=60)
                                                                                TACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATG             <  SRR4302540.8128347/94‑1 (MQ=60)
                                                                                TACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATG             <  SRR4302540.1573761/94‑1 (MQ=60)
                                                                                TACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATG             >  SRR4302540.8711221/1‑94 (MQ=60)
                                                                                TACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATG             <  SRR4302540.6977123/94‑1 (MQ=60)
                                                                                 ACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGT            >  SRR4302540.5655732/1‑94 (MQ=60)
                                                                                 ACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGA            >  SRR4302540.5956067/1‑94 (MQ=60)
                                                                                 ACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGA            >  SRR4302540.5655733/1‑94 (MQ=60)
                                                                                 ACCAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGA            <  SRR4302540.2559865/94‑1 (MQ=60)
                                                                                   CAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATG          >  SRR4302540.7269561/1‑94 (MQ=60)
                                                                                   CAGTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATG          >  SRR4302540.7727923/1‑94 (MQ=60)
                                                                                     GTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGCGATATCCATGCTGGGTTCCCTTACCTTACAAATAATCCATGATGTT        >  SRR4302540.1618329/1‑94 (MQ=60)
                                                                                     GTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTT        >  SRR4302540.1618328/1‑94 (MQ=60)
                                                                                     GTC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTT        >  SRR4302540.2611351/1‑94 (MQ=60)
                                                                                      TC‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTT       <  SRR4302540.5417018/94‑1 (MQ=60)
                                                                                       C‑GGGGGGCTGTCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTT      <  SRR4302540.9297513/94‑1 (MQ=60)
                                                                                       C‑GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTT      >  SRR4302540.2921785/1‑94 (MQ=60)
                                                                                         GGGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTT     >  SRR4302540.797360/1‑94 (MQ=60)
                                                                                          GGGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTA    <  SRR4302540.906364/94‑1 (MQ=60)
                                                                                          GGGGCCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTA    <  SRR4302540.35721/94‑1 (MQ=60)
                                                                                           GGGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTAT   <  SRR4302540.3017904/94‑1 (MQ=60)
                                                                                            GGGCTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTATG  <  SRR4302540.8831179/94‑1 (MQ=60)
                                                                                              |                                                                                           
CCAGCAACCAGCCCGCGACGAAAGGGCTGATGAGGAAAATTAACGGGTTTACGATTAAGAAAATGATGAGGGCGAGTTTGTACCAGTC‑GGGGGACTGGCCCAAAAAATTGCGCCATAGCGCGCGGCCCCAGGAGATCTCCATGATGGTTTCCCTTACCTTACAAATAATCAATGATGTTTTTATG  >  NC_000913/1233799‑1233983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑