Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,237,781 A→G T330A (ACG→GCG)  dadA → D‑amino acid dehydrogenase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,237,7810AG99.2% 780.3 / ‑2.9 247T330A (ACG→GCG) dadAD‑amino acid dehydrogenase subunit
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (112/133);  total (112/135)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.56e-01

Only 100 of 256 total aligned reads displayed.

GCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCCA  >  NC_000913/1237691‑1237870
                                                                                          |                                                                                         
gCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCg                                                                                         >  2:7946786/1‑93 (MQ=255)
  cATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCt                                                                                       >  1:9198859/1‑93 (MQ=255)
  cATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCt                                                                                       <  1:9170511/93‑1 (MQ=255)
  cATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCt                                                                                       <  2:1925306/93‑1 (MQ=255)
  cATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCt                                                                                       <  2:2538830/93‑1 (MQ=255)
  cATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCt                                                                                       <  1:1581504/93‑1 (MQ=255)
   aTTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTg                                                                                      >  1:2175283/1‑93 (MQ=255)
   aTTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTg                                                                                      >  2:2153200/1‑93 (MQ=255)
   aTTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTg                                                                                      >  1:3880395/1‑93 (MQ=255)
    ttACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGCGCTGTTGCAACCGCGTCGCGAAGCCCTgg                                                                                     >  1:3279527/1‑93 (MQ=255)
    ttACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTgg                                                                                     >  1:428254/1‑93 (MQ=255)
     tACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGga                                                                                    <  1:7872984/93‑1 (MQ=255)
       cccGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGgaga                                                                                  <  2:9734413/93‑1 (MQ=255)
        ccGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGAt                                                                                 <  1:510884/93‑1 (MQ=255)
        ccGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGAt                                                                                 <  1:7638684/93‑1 (MQ=255)
         cGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGAtg                                                                                <  1:8997700/93‑1 (MQ=255)
           tttCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGAtggt                                                                              >  2:1810882/1‑93 (MQ=255)
           tttCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGAtggt                                                                              >  1:2416506/1‑93 (MQ=255)
           tttCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGAtggt                                                                              <  1:8026935/93‑1 (MQ=255)
            ttCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGAtggtg                                                                             <  1:2630098/93‑1 (MQ=255)
             tCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTCCAACCGCGTCGTGAAGCGCTGGAGAtggtag                                                                            <  2:5701289/93‑3 (MQ=255)
               gATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGtt                                                                          >  2:6403646/1‑93 (MQ=255)
                aTAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGttg                                                                         >  1:4888725/1‑92 (MQ=255)
                aTAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTc                                                                         >  2:3718141/1‑93 (MQ=255)
                aTAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTc                                                                         >  2:357094/1‑93 (MQ=255)
                  aaCCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTcgc                                                                       <  1:1412113/93‑1 (MQ=255)
                    ccGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGa                                                                     <  2:8420492/93‑1 (MQ=255)
                     cGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGAt                                                                    <  2:3271108/93‑1 (MQ=255)
                      gCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGagc                                                                   >  2:506983/1‑91 (MQ=255)
                      gCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGAtc                                                                   <  2:9165451/93‑1 (MQ=255)
                      gCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGAtc                                                                   >  1:8459128/1‑93 (MQ=255)
                       cATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGAtct                                                                  >  1:3709778/1‑93 (MQ=255)
                        aTTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGAtctc                                                                 >  1:6625214/1‑93 (MQ=255)
                        aTTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGAtctc                                                                 <  1:9554127/93‑1 (MQ=255)
                         ttCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATctct                                                                >  1:7266726/1‑93 (MQ=255)
                           cGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTAt                                                              <  2:3348277/93‑1 (MQ=255)
                            gtgtTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATc                                                             <  2:5861926/93‑1 (MQ=255)
                             tgtTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATcc                                                            <  2:5765188/93‑1 (MQ=255)
                              gtTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCTAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCa                                                           <  2:716190/93‑1 (MQ=255)
                                 ggCGGAATGGCGGAGATTGTTGGTGTGAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCAcgc                                                        <  1:9615277/93‑1 (MQ=255)
                                   cGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACgcgg                                                      <  2:428254/93‑1 (MQ=255)
                                    ggAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACgcggc                                                     <  2:2938859/93‑1 (MQ=255)
                                    ggAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACgcggc                                                     <  2:4871855/93‑1 (MQ=255)
                                    ggAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACgcggc                                                     <  1:4504900/93‑1 (MQ=255)
                                     gAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACgcggcg                                                    <  2:8322434/93‑1 (MQ=255)
                                       aTGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGt                                                  >  1:4658022/1‑93 (MQ=255)
                                       aTGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGt                                                  >  2:231998/1‑93 (MQ=255)
                                         ggCGTAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCa                                                <  1:708282/93‑1 (MQ=255)
                                         ggCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCa                                                >  1:5491737/1‑93 (MQ=255)
                                         ggCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCa                                                <  2:2444530/93‑1 (MQ=255)
                                          gCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCAt                                               <  1:3482075/93‑1 (MQ=255)
                                          gCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCAt                                               >  1:557051/1‑93 (MQ=255)
                                          gCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCAt                                               <  1:7802540/93‑1 (MQ=255)
                                          gCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCAt                                               >  1:5771286/1‑93 (MQ=255)
                                             gagaTTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTc                                            <  2:9566807/93‑1 (MQ=255)
                                             gagaTTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTc                                            >  2:2652541/1‑93 (MQ=255)
                                              agaTTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCg                                           <  1:5960071/93‑1 (MQ=255)
                                               gaTTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGa                                          <  1:8207302/93‑1 (MQ=255)
                                               gaTTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGa                                          >  2:6818353/1‑93 (MQ=255)
                                               gaTTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGa                                          >  2:6033720/1‑93 (MQ=255)
                                               gaTTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGa                                          >  1:6579070/1‑93 (MQ=255)
                                                  tgttgGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCa                                       <  2:416920/93‑1 (MQ=255)
                                                   gttgGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAg                                      <  1:6513242/93‑1 (MQ=255)
                                                     tgGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGc                                    <  1:9710352/93‑1 (MQ=255)
                                                        ttttAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGAc                                 >  1:8688418/1‑93 (MQ=255)
                                                         tttAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACt                                <  2:2815456/93‑1 (MQ=255)
                                                         tttAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGCCGGCATTGTCGAGCAGGCGACt                                >  1:7293611/1‑93 (MQ=255)
                                                           tAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACttt                              >  1:2961666/1‑93 (MQ=255)
                                                            aaTACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTc                             <  2:3445611/93‑1 (MQ=255)
                                                             aTACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCt                            >  1:6761883/1‑93 (MQ=255)
                                                             aTACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCt                            <  2:9065972/93‑1 (MQ=255)
                                                              tACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTg                           >  2:7346532/1‑93 (MQ=255)
                                                               aCCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTgg                          >  2:4239950/1‑93 (MQ=255)
                                                                ccGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGa                         <  2:7857931/93‑1 (MQ=255)
                                                                ccGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGa                         >  1:9922866/1‑93 (MQ=255)
                                                                ccGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTATATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGa                         >  1:1309911/1‑93 (MQ=255)
                                                                 cGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGAc                        <  2:3861648/93‑1 (MQ=255)
                                                                 cGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGAc                        <  1:8069115/93‑1 (MQ=255)
                                                                 cGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGAc                        <  1:10045763/93‑1 (MQ=255)
                                                                   aGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTg                      <  1:708427/93‑1 (MQ=255)
                                                                    gCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTgg                     <  1:3096032/93‑1 (MQ=255)
                                                                     cTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCTAGCAGGCGACTTTCTGGCCTGGt                    >  2:5537638/1‑93 (MQ=255)
                                                                     cTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGt                    >  2:9010233/1‑93 (MQ=255)
                                                                      tGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTc                   >  2:5424836/1‑93 (MQ=255)
                                                                      tGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTc                   >  1:1834734/1‑93 (MQ=255)
                                                                      tGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTc                   <  1:6214789/93‑1 (MQ=255)
                                                                      tGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTc                   <  2:3391104/93‑1 (MQ=255)
                                                                        ttGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTg                 >  2:7342643/1‑93 (MQ=255)
                                                                        tctcAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTg                 >  2:1562371/4‑93 (MQ=255)
                                                                           cacccGCGTCGTGCAGCGCTGGAGCTGGTGGTTCGCGATCTCTATCCCCGCGGCGGTCATGTCGAGCAGGCGCCTTTCTGGCCTGGTCTgcgc              <  2:9263711/90‑1 (MQ=255)
                                                                               cgcgTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGTGACTTTCTGGACTGGTCTGCGCCCGa          <  1:1121045/93‑1 (MQ=255)
                                                                                gcgTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGAt         >  2:4185974/1‑93 (MQ=255)
                                                                                gcgTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGAt         <  2:961801/93‑1 (MQ=255)
                                                                                gcgTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGAt         >  1:9112801/1‑93 (MQ=255)
                                                                                gcgTCGTGAAGCGCTGGAGATGGAGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGAt         >  2:2341055/1‑93 (MQ=255)
                                                                                  gtcgtGAAACGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGa       <  2:6964988/93‑1 (MQ=255)
                                                                                    cgtGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACg     <  2:8565110/93‑1 (MQ=255)
                                                                                     gtGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGc    >  2:1362709/1‑93 (MQ=255)
                                                                                     gtGAAGAGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGCCTTTCTGGACTGGTCTGCCCCCGATGAAGc    >  2:1113459/1‑93 (MQ=255)
                                                                                      tGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGcc   >  2:1697034/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCCA  >  NC_000913/1237691‑1237870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCCAGACG  >  NC_000913/1237688‑1237874
                                                                                             |                                                                                             
ATCGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAG                                                                                               <  SRR4302540.1447835/94‑1 (MQ=60)
 TCCCCATTACCCGTTTCGATAACCGCATTCGTTGTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGC                                                                                              <  SRR4302540.5158589/94‑1 (MQ=60)
 ACGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGC                                                                                              <  SRR4302540.3644977/94‑1 (MQ=60)
 ACGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTAGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGC                                                                                              <  SRR4302540.6433861/94‑1 (MQ=60)
  CGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCG                                                                                             <  SRR4302540.1898218/94‑1 (MQ=60)
  CGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCG                                                                                             <  SRR4302540.8827917/94‑1 (MQ=60)
  CGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCG                                                                                             <  SRR4302540.8303201/94‑1 (MQ=60)
   GCCATTACCCGTTTCGATAACCGCATTCGTGTTGGAGGAATGGCGGAGATTGTTGGTTTTAATACCGAGATGTTGCAACCGCGTCGTGAAGCGC                                                                                            >  SRR4302540.3638262/1‑94 (MQ=60)
    CCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCT                                                                                           >  SRR4302540.9309209/1‑94 (MQ=60)
    CCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCT                                                                                           <  SRR4302540.9233224/94‑1 (MQ=60)
     CATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTG                                                                                          <  SRR4302540.9280518/94‑1 (MQ=60)
     CATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTG                                                                                          >  SRR4302540.2198739/1‑94 (MQ=60)
     CATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTG                                                                                          <  SRR4302540.1598784/94‑1 (MQ=60)
     CATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTG                                                                                          >  SRR4302540.3922558/1‑94 (MQ=60)
      ATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGCGCTGTTGCAACCGCGTCGCGAAGCCCTGG                                                                                         >  SRR4302540.3315348/1‑94 (MQ=60)
      ATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGG                                                                                         >  SRR4302540.3228481/1‑94 (MQ=60)
      ATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGG                                                                                         >  SRR4302540.432757/1‑94 (MQ=60)
       TTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGA                                                                                        <  SRR4302540.2434578/94‑1 (MQ=60)
       TTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGA                                                                                        <  SRR4302540.3715936/94‑1 (MQ=60)
       TTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGA                                                                                        <  SRR4302540.6060899/94‑1 (MQ=60)
        TACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAG                                                                                       <  SRR4302540.7964948/94‑1 (MQ=60)
          CCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGAT                                                                                     <  SRR4302540.377458/94‑1 (MQ=60)
          CCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGAT                                                                                     <  SRR4302540.3943801/94‑1 (MQ=60)
          CCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGAT                                                                                     <  SRR4302540.7557152/94‑1 (MQ=60)
           CCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATG                                                                                    <  SRR4302540.516374/94‑1 (MQ=60)
           CCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATG                                                                                    <  SRR4302540.7724638/94‑1 (MQ=60)
            CGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGG                                                                                   <  SRR4302540.9105640/94‑1 (MQ=60)
            CGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGG                                                                                   <  SRR4302540.7366218/94‑1 (MQ=60)
            CGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGG                                                                                   <  SRR4302540.3231685/94‑1 (MQ=60)
             GTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGT                                                                                  >  SRR4302540.2442547/1‑94 (MQ=60)
              TTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTG                                                                                 <  SRR4302540.8120279/94‑1 (MQ=60)
               TTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGG                                                                                <  SRR4302540.2658564/94‑1 (MQ=60)
                 CGATATCCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTT                                                                              <  SRR4302540.6391175/94‑1 (MQ=60)
                 CGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTT                                                                              >  SRR4302540.4812306/1‑94 (MQ=60)
                 CGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTGGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTT                                                                              <  SRR4302540.7162456/94‑1 (MQ=60)
                  GATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTG                                                                             >  SRR4302540.4941272/1‑94 (MQ=60)
                  GATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTC                                                                             >  SRR4302540.6577504/1‑94 (MQ=60)
                  GATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTC                                                                             <  SRR4302540.8765232/94‑1 (MQ=60)
                   ATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCG                                                                            >  SRR4302540.5173660/1‑94 (MQ=60)
                   ATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGCTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTGCG                                                                            >  SRR4302540.7883323/1‑94 (MQ=60)
                     AACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCG                                                                          <  SRR4302540.1427347/94‑1 (MQ=60)
                       CCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGAT                                                                        >  SRR4302540.9773545/1‑94 (MQ=60)
                        CGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATC                                                                       >  SRR4302540.8557345/1‑94 (MQ=60)
                        CGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATC                                                                       <  SRR4302540.5678613/94‑1 (MQ=60)
                         GCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCT                                                                      >  SRR4302540.4218525/1‑94 (MQ=60)
                         GCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCT                                                                      >  SRR4302540.3750266/1‑94 (MQ=60)
                          CATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTC                                                                     >  SRR4302540.6698562/1‑94 (MQ=60)
                          CATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTC                                                                     <  SRR4302540.7483199/94‑1 (MQ=60)
                          CATTCGTGTTGGCGGAATGGCGGAGATTGGTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTC                                                                     <  SRR4302540.8887086/94‑1 (MQ=60)
                           ATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCT                                                                    <  SRR4302540.9667125/94‑1 (MQ=60)
                           ATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCT                                                                    >  SRR4302540.7347806/1‑94 (MQ=60)
                           ATTCGAGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGCGATGGTGGTTCGCGATCTCT                                                                    >  SRR4302540.6635925/1‑94 (MQ=60)
                                TGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCA                                                               <  SRR4302540.7186806/94‑1 (MQ=60)
                                 TTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCAC                                                              <  SRR4302540.8660042/94‑1 (MQ=60)
                                 GTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCCGTTGCAACCGCGTCGTGAAGTGCTAGAGATGGTGGTTCGCGATCTCTATCCAA                                                              >  SRR4302540.7121090/1‑94 (MQ=60)
                                    GGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCG                                                           <  SRR4302540.1333853/94‑1 (MQ=60)
                                    GGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCG                                                           >  SRR4302540.10075270/1‑94 (MQ=60)
                                    GGCGGAATGGCGGAGATTGTTGGTGTGAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCG                                                           <  SRR4302540.9728639/94‑1 (MQ=60)
                                     GCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGG                                                          <  SRR4302540.1438805/94‑1 (MQ=60)
                                      CGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGC                                                         <  SRR4302540.4954778/94‑1 (MQ=60)
                                       GGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCG                                                        <  SRR4302540.4553728/94‑1 (MQ=60)
                                       GGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCG                                                        >  SRR4302540.9666170/1‑94 (MQ=60)
                                       GGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCG                                                        <  SRR4302540.7843238/94‑1 (MQ=60)
                                       GGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCG                                                        >  SRR4302540.5726682/1‑94 (MQ=60)
                                        GAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGG                                                       >  SRR4302540.9201309/1‑94 (MQ=60)
                                         AATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGT                                                      >  SRR4302540.4707876/1‑94 (MQ=60)
                                         AATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGC                                                      >  SRR4302540.1037172/1‑94 (MQ=60)
                                          ATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTC                                                     >  SRR4302540.9286852/1‑94 (MQ=60)
                                          ATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTC                                                     >  SRR4302540.9187989/1‑94 (MQ=60)
                                          ATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTC                                                     >  SRR4302540.4538650/1‑94 (MQ=60)
                                          ATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTC                                                     >  SRR4302540.8517264/1‑94 (MQ=60)
                                           TGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCA                                                    >  SRR4302540.5552200/1‑94 (MQ=60)
                                            tggcgacattcttgtTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGAGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCAT                                                   <  SRR4302540.8029680/79‑1 (MQ=60)
                                            GGCGTAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCAT                                                   <  SRR4302540.715898/94‑1 (MQ=60)
                                            GGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCAT                                                   >  SRR4302540.5835026/1‑94 (MQ=60)
                                            GGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCAT                                                   >  SRR4302540.563119/1‑94 (MQ=60)
                                            GGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCAT                                                   <  SRR4302540.6758484/94‑1 (MQ=60)
                                             GCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATG                                                  <  SRR4302540.3519987/94‑1 (MQ=60)
                                             GCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATG                                                  <  SRR4302540.7893732/94‑1 (MQ=60)
                                             GCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATG                                                  >  SRR4302540.3384291/1‑94 (MQ=60)
                                                 AGATTGTTGGTTTTTATCCCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGA                                              <  SRR4302540.6025998/94‑1 (MQ=60)
                                                 AGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGA                                              >  SRR4302540.6651945/1‑94 (MQ=60)
                                                 AGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGA                                              >  SRR4302540.1701297/1‑94 (MQ=60)
                                                 AGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGA                                              <  SRR4302540.6025999/94‑1 (MQ=60)
                                                 AGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTAATCACGTGGCGGTCATGTCGA                                              >  SRR4302540.6651946/1‑94 (MQ=60)
                                                  GATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAG                                             <  SRR4302540.788951/94‑1 (MQ=60)
                                                  GATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAG                                             <  SRR4302540.8302673/94‑1 (MQ=60)
                                                    TTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCG                                           >  SRR4302540.6977745/1‑94 (MQ=60)
                                                      GTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGG                                         <  SRR4302540.6585596/94‑1 (MQ=60)
                                                       TTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGC                                        <  SRR4302540.5576162/94‑1 (MQ=60)
                                                       TTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGC                                        <  SRR4302540.4809865/94‑1 (MQ=60)
                                                        TGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCG                                       <  SRR4302540.9824475/94‑1 (MQ=60)
                                                         GGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGA                                      <  SRR4302540.7335170/94‑1 (MQ=60)
                                                          GTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGAC                                     >  SRR4302540.8789298/1‑94 (MQ=60)
                                                          GTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGAC                                     >  SRR4302540.6680090/1‑94 (MQ=60)
                                                           TTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGCCGGCATTGTCGAGCAGGCGACT                                    >  SRR4302540.7375011/1‑94 (MQ=60)
                                                            TTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGCCTGCGATCTCTATCCACTCTGCGGTCATGTCGAGCAGGCGACTT                                   >  SRR4302540.5215008/1‑94 (MQ=60)
                                                             TTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTT                                  >  SRR4302540.2994321/1‑94 (MQ=60)
                                                             TTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGGCATGTCGAGCGGGCGACTTT                                  >  SRR4302540.2589832/1‑94 (MQ=60)
                                                              TAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTC                                 >  SRR4302540.7980166/1‑94 (MQ=60)
                                                               AATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCT                                >  SRR4302540.6836613/1‑94 (MQ=60)
                                                                ATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTG                               >  SRR4302540.2992233/1‑94 (MQ=60)
                                                                  ACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGA                             >  SRR4302540.627850/1‑94 (MQ=60)
                                                                  ACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGA                             >  SRR4302540.10038730/1‑94 (MQ=60)
                                                                  ACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTATATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGA                             >  SRR4302540.1323518/1‑94 (MQ=60)
                                                                    CGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACT                           <  SRR4302540.10162546/94‑1 (MQ=60)
                                                                    CGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACT                           >  SRR4302540.8150680/1‑94 (MQ=60)
                                                                    CGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACT                           <  SRR4302540.8162742/94‑1 (MQ=60)
                                                                     GAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTG                          <  SRR4302540.2495058/94‑1 (MQ=60)
                                                                      AGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGG                         <  SRR4302540.9719570/94‑1 (MQ=60)
                                                                      AGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGG                         <  SRR4302540.716043/94‑1 (MQ=60)
                                                                       GCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGT                        <  SRR4302540.3129967/94‑1 (MQ=60)
                                                                        CTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTC                       >  SRR4302540.1854812/1‑94 (MQ=60)
                                                                         TGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCT                      <  SRR4302540.6283937/94‑1 (MQ=60)
                                                                            TGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCG                   <  SRR4302540.4606131/94‑1 (MQ=60)
                                                                              CAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCC                 <  SRR4302540.1215858/94‑1 (MQ=60)
                                                                               cgCCGCGTCGTGAAGCGCTGGAGATGTTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCC                <  SRR4302540.2010445/92‑1 (MQ=60)
                                                                               AACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCC                >  SRR4302540.6932893/1‑94 (MQ=60)
                                                                               AACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCC                <  SRR4302540.3073553/94‑1 (MQ=60)
                                                                               AACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCC                >  SRR4302540.1475990/1‑94 (MQ=60)
                                                                               AACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCC                >  SRR4302540.46802/1‑94 (MQ=60)
                                                                                ACCGCGTCATGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCG               <  SRR4302540.10014205/94‑1 (MQ=60)
                                                                                  CGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGTGACTTTCTGGACTGGTCTGCGCCCGAT             <  SRR4302540.1132679/94‑1 (MQ=60)
                                                                                  CGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGAT             >  SRR4302540.9222322/1‑94 (MQ=60)
                                                                                  CGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGAT             >  SRR4302540.9222323/1‑94 (MQ=60)
                                                                                    CGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGA           <  SRR4302540.2027428/94‑1 (MQ=60)
                                                                                     GTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGCCTTTCTGGACTGGTCTGCGCCCGATGAC          <  SRR4302540.1136316/94‑1 (MQ=60)
                                                                                     GTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGAC          <  SRR4302540.8002062/94‑1 (MQ=60)
                                                                                     GTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGAC          >  SRR4302540.542396/1‑94 (MQ=60)
                                                                                     GTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGAC          >  SRR4302540.9627187/1‑94 (MQ=60)
                                                                                     GTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGAC          <  SRR4302540.3617907/94‑1 (MQ=60)
                                                                                        GTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCC       <  SRR4302540.8286808/94‑1 (MQ=60)
                                                                                        GTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCC       <  SRR4302540.9818457/94‑1 (MQ=60)
                                                                                        GTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCC       >  SRR4302540.8292316/1‑94 (MQ=60)
                                                                                          GAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCCAG     <  SRR4302540.28986/94‑1 (MQ=60)
                                                                                          GAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCCAG     >  SRR4302540.8215202/1‑94 (MQ=60)
                                                                                          GAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCCAG     >  SRR4302540.6726206/1‑94 (MQ=60)
                                                                                           AAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCCAGA    >  SRR4302540.3634576/1‑94 (MQ=60)
                                                                                            AGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCCAGAC   <  SRR4302540.10026773/94‑1 (MQ=60)
                                                                                             GCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCCAGACG  >  SRR4302540.9600742/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATCGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCCAGACG  >  NC_000913/1237688‑1237874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑