Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,237,781 A→G T330A (ACG→GCG)  dadA → D‑amino acid dehydrogenase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,237,7810AG100.0% 604.7 / NA 186T330A (ACG→GCG) dadAD‑amino acid dehydrogenase subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (109/77);  total (109/77)

Only 100 of 194 total aligned reads displayed.

GCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCCA  >  NC_000913/1237691‑1237870
                                                                                          |                                                                                         
gCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCg                                                                                         >  1:5621307/1‑93 (MQ=255)
gCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCg                                                                                         <  2:146683/93‑1 (MQ=255)
 ccATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAgcgc                                                                                        >  1:5439921/1‑93 (MQ=255)
  cATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCt                                                                                       >  2:4363587/1‑93 (MQ=255)
   aTTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTg                                                                                      <  2:712010/93‑1 (MQ=255)
   aTTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAAACGCGTCGTGAAGCGCTg                                                                                      >  2:4512767/1‑93 (MQ=255)
    ttACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTgg                                                                                     >  2:2570719/1‑93 (MQ=255)
     tACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGga                                                                                    >  1:474632/1‑93 (MQ=255)
      aCCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGGGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGgag                                                                                   >  2:5647801/1‑93 (MQ=255)
      aCCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGgag                                                                                   >  2:170111/1‑93 (MQ=255)
      aCCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGgag                                                                                   >  2:5355843/1‑93 (MQ=255)
       cccGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGgaga                                                                                  <  1:5006948/93‑1 (MQ=255)
       cccGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGgaga                                                                                  >  1:5708944/1‑93 (MQ=255)
        ccGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGAt                                                                                 <  2:2499246/93‑1 (MQ=255)
        ccGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGAt                                                                                 <  1:2908677/93‑1 (MQ=255)
         cGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGCGAtg                                                                                >  1:3753294/1‑93 (MQ=255)
          gTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGAtgg                                                                               <  1:1965570/93‑1 (MQ=255)
           tttCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGAtggt                                                                              >  2:3319696/1‑93 (MQ=255)
           tttCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGAtggt                                                                              >  2:5943394/1‑93 (MQ=255)
           tttCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGAtggg                                                                              >  1:672198/1‑92 (MQ=255)
            ttCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGAtggtg                                                                             <  2:4017991/93‑1 (MQ=255)
             tCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGAtggtgg                                                                            <  1:856562/93‑1 (MQ=255)
               gATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGtt                                                                          >  1:5688844/1‑93 (MQ=255)
               gATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGtt                                                                          >  2:2526507/1‑93 (MQ=255)
               gATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGtt                                                                          >  1:3901111/1‑93 (MQ=255)
                aTAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTc                                                                         <  1:1081809/93‑1 (MQ=255)
                aTAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTc                                                                         <  1:3785038/93‑1 (MQ=255)
                 tAACCGCATTCGTGTTGGCGGTATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGGTGGAGATGGAGGTTcg                                                                        >  2:2791845/1‑93 (MQ=255)
                  aaCCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTcgc                                                                       <  2:2717256/93‑1 (MQ=255)
                   aCCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTcgcg                                                                      <  2:6228209/93‑1 (MQ=255)
                   aCCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTcgcg                                                                      >  2:3814648/1‑93 (MQ=255)
                       cATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGAtct                                                                  >  2:5961864/1‑93 (MQ=255)
                        aTTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGAtctc                                                                 <  1:6220572/93‑1 (MQ=255)
                        aTTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGAtctc                                                                 <  1:3550008/93‑1 (MQ=255)
                        aTTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGAtctc                                                                 <  2:486729/93‑1 (MQ=255)
                        aTTCATGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGAtctc                                                                 >  2:1007821/1‑93 (MQ=255)
                         ttCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATctct                                                                >  2:1322005/1‑93 (MQ=255)
                           cGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTAt                                                              <  2:6272997/93‑1 (MQ=255)
                             tgtTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATcc                                                            >  1:4394329/1‑93 (MQ=255)
                             tgtTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTAGAGATGGTGGTTCGCGATCTCTATca                                                            >  1:5519861/1‑92 (MQ=255)
                              gtTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCa                                                           >  1:122791/1‑93 (MQ=255)
                              gtTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCa                                                           <  1:1974026/93‑1 (MQ=255)
                               ttGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCAc                                                          <  2:4147760/93‑1 (MQ=255)
                                  gCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCAcgcg                                                       <  1:1688244/93‑1 (MQ=255)
                                  gCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCAcgcg                                                       >  2:5790965/1‑93 (MQ=255)
                                   gggAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACgcgg                                                      <  1:2997268/92‑1 (MQ=255)
                                   cGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACgcgg                                                      <  2:3028438/93‑1 (MQ=255)
                                   cGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACgcgg                                                      <  1:4353027/93‑1 (MQ=255)
                                    ggAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACgcggc                                                     >  2:2859368/1‑93 (MQ=255)
                                     gAATGGCGGAGTTTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACgcggcg                                                    <  1:329302/93‑1 (MQ=255)
                                     gAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACgcggcg                                                    <  2:2103424/93‑1 (MQ=255)
                                       aTGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGt                                                  >  1:2419674/1‑93 (MQ=255)
                                        tGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTc                                                 >  2:3704349/1‑93 (MQ=255)
                                        tGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTc                                                 <  1:5126468/93‑1 (MQ=255)
                                        tGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTc                                                 <  1:4878313/93‑1 (MQ=255)
                                           cGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATg                                              >  2:3899162/1‑93 (MQ=255)
                                            ggAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGt                                             >  2:3142473/1‑93 (MQ=255)
                                            ggAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGt                                             >  1:5347639/1‑93 (MQ=255)
                                            ggAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGt                                             <  1:4850694/93‑1 (MQ=255)
                                             gataTTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTc                                            <  1:4632912/90‑1 (MQ=255)
                                             gagaTTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTc                                            <  2:1651279/93‑1 (MQ=255)
                                              agaTTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCg                                           >  2:3286117/1‑93 (MQ=255)
                                              agaTTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCg                                           >  2:1186094/1‑93 (MQ=255)
                                               gaTTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGa                                          <  1:1302327/93‑1 (MQ=255)
                                               gaTTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGa                                          >  2:1885150/1‑93 (MQ=255)
                                                aTTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAg                                         <  2:4198700/93‑1 (MQ=255)
                                                aTTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAg                                         >  2:1517453/1‑93 (MQ=255)
                                                 ttgttgGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGc                                        >  2:1803027/1‑93 (MQ=255)
                                                  tgttgGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCa                                       <  2:3488262/93‑1 (MQ=255)
                                                   gttgGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAg                                      >  2:2661884/1‑93 (MQ=255)
                                                   gttgGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAg                                      <  1:5767409/93‑1 (MQ=255)
                                                    ttgGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAgg                                     >  1:5064356/1‑93 (MQ=255)
                                                    ttgGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAgg                                     <  2:1126251/93‑1 (MQ=255)
                                                      ggTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCg                                   <  1:4709676/93‑1 (MQ=255)
                                                       gTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGa                                  >  1:2581409/1‑93 (MQ=255)
                                                           tAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACttt                              >  1:3835388/1‑93 (MQ=255)
                                                            aaTACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTc                             <  2:5015309/93‑1 (MQ=255)
                                                             aTACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCt                            <  1:4756825/93‑1 (MQ=255)
                                                             aTACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCt                            >  2:2911153/1‑93 (MQ=255)
                                                              tACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGACATGTCGAGCAGGCGAGTTTCTg                           >  1:3443606/1‑93 (MQ=255)
                                                               aCCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTgg                          >  2:68802/1‑93 (MQ=255)
                                                               aCCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTgg                          >  1:1509758/1‑93 (MQ=255)
                                                                 cGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGAc                        >  1:105918/1‑93 (MQ=255)
                                                                    gCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTgg                     >  2:2194452/1‑93 (MQ=255)
                                                                       gTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCt                  >  1:96431/1‑93 (MQ=255)
                                                                       gTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCt                  >  2:1603252/1‑93 (MQ=255)
                                                                        ttGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTg                 >  2:4295519/1‑93 (MQ=255)
                                                                         tGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTgc                <  1:880932/93‑1 (MQ=255)
                                                                             aCCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGgcc            >  1:1245867/1‑93 (MQ=255)
                                                                             aCCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGccc            >  1:1535226/1‑93 (MQ=255)
                                                                               cgcgTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGa          <  1:2816364/93‑1 (MQ=255)
                                                                                gcgTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGag         >  1:5270874/1‑92 (MQ=255)
                                                                                gcgTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGAt         >  1:4652002/1‑93 (MQ=255)
                                                                                 cgtcgtGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATg        >  2:73831/1‑93 (MQ=255)
                                                                                  gtcgtGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATgc       >  1:2058395/1‑92 (MQ=255)
                                                                                  gtcgtGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGa       <  1:6005512/93‑1 (MQ=255)
                                                                                   tcgtGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGAc      >  1:4083340/1‑93 (MQ=255)
                                                                                   tcgtGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGAc      >  1:5324702/1‑93 (MQ=255)
                                                                                      tGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGcc   >  1:4162196/1‑93 (MQ=255)
                                                                                       gAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCCa  >  1:3340560/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCCA  >  NC_000913/1237691‑1237870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCCAGACG  >  NC_000913/1237688‑1237874
                                                                                             |                                                                                             
CTCGCCATTACCCGTTTCGATAACCGCGTTCGTGTTGACGGAATGGAGGGGTTTGTTGATTTATAAACCAAGCTGTTGCAACCGCGTCGTGAAG                                                                                               <  SRR4302533.6337785/94‑1 (MQ=38)
ATCGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAG                                                                                               >  SRR4302533.5008552/1‑94 (MQ=60)
  CGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCG                                                                                             >  SRR4302533.5655285/1‑94 (MQ=60)
  CGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCG                                                                                             <  SRR4302533.2941403/94‑1 (MQ=60)
  CGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCG                                                                                             <  SRR4302533.1735007/94‑1 (MQ=60)
  CGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCG                                                                                             <  SRR4302533.957560/94‑1 (MQ=60)
   GCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGC                                                                                            >  SRR4302533.5472681/1‑94 (MQ=60)
    CCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCT                                                                                           >  SRR4302533.5195683/1‑94 (MQ=60)
      ATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGG                                                                                         >  SRR4302533.5511151/1‑94 (MQ=60)
       TTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGA                                                                                        >  SRR4302533.5463859/1‑94 (MQ=60)
       TTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGA                                                                                        >  SRR4302533.477467/1‑94 (MQ=60)
       TTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGA                                                                                        <  SRR4302533.3613120/94‑1 (MQ=60)
         ACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGA                                                                                      >  SRR4302533.5743426/1‑94 (MQ=60)
          CCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGAT                                                                                     <  SRR4302533.5037288/94‑1 (MQ=60)
           CCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGCGATG                                                                                    >  SRR4302533.3776300/1‑94 (MQ=60)
           CCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATG                                                                                    <  SRR4302533.5734074/94‑1 (MQ=60)
           CCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATG                                                                                    <  SRR4302533.4771671/94‑1 (MQ=60)
           CCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATG                                                                                    <  SRR4302533.2926532/94‑1 (MQ=60)
            CGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGG                                                                                   >  SRR4302533.287942/1‑94 (MQ=60)
             GTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGT                                                                                  <  SRR4302533.1977578/94‑1 (MQ=60)
             GTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGG                                                                                  >  SRR4302533.676166/1‑94 (MQ=60)
             GTTTCGATAACCGCAGTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGT                                                                                  <  SRR4302533.1977579/94‑1 (MQ=60)
              TTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTG                                                                                 <  SRR4302533.1200736/94‑1 (MQ=60)
                TCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGT                                                                               <  SRR4302533.861577/94‑1 (MQ=60)
                TCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGT                                                                               <  SRR4302533.3464384/94‑1 (MQ=60)
                 CGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTT                                                                              >  SRR4302533.5723205/1‑94 (MQ=60)
                 CGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTT                                                                              <  SRR4302533.2017933/94‑1 (MQ=60)
                 CGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTT                                                                              >  SRR4302533.3924926/1‑94 (MQ=60)
                  GATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTC                                                                             >  SRR4302533.4570363/1‑94 (MQ=60)
                   ATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCG                                                                            <  SRR4302533.3931974/94‑1 (MQ=60)
                   ATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCG                                                                            >  SRR4302533.5130504/1‑94 (MQ=60)
                   ATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCG                                                                            <  SRR4302533.3808212/94‑1 (MQ=60)
                   ATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCG                                                                            <  SRR4302533.1088191/94‑1 (MQ=60)
                     AACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCG                                                                          >  SRR4302533.3902954/1‑94 (MQ=60)
                     AACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCG                                                                          <  SRR4302533.1598475/94‑1 (MQ=60)
                       CCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGAT                                                                        >  SRR4302533.1004796/1‑94 (MQ=60)
                         GCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCT                                                                      <  SRR4302533.899417/94‑1 (MQ=60)
                           ATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCT                                                                    <  SRR4302533.3571878/94‑1 (MQ=60)
                           ATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCT                                                                    >  SRR4302533.4862500/1‑94 (MQ=60)
                           ATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCT                                                                    <  SRR4302533.6257863/94‑1 (MQ=60)
                            TTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTA                                                                   <  SRR4302533.1247261/94‑1 (MQ=60)
                             TCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTAT                                                                  <  SRR4302533.680060/94‑1 (MQ=60)
                              CGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATC                                                                 >  SRR4302533.4784202/1‑94 (MQ=60)
                               GTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCC                                                                >  SRR4302533.4420861/1‑94 (MQ=60)
                               GTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTAGAGATGGTGGTTCGCGATCTCTATCA                                                                >  SRR4302533.5553191/1‑94 (MQ=60)
                                TGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCA                                                               >  SRR4302533.123564/1‑94 (MQ=60)
                                 GTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCAC                                                              <  SRR4302533.3558736/94‑1 (MQ=60)
                                 GTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCAC                                                              >  SRR4302533.2536880/1‑94 (MQ=60)
                                 GTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCAC                                                              <  SRR4302533.1986112/94‑1 (MQ=60)
                                   TGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGC                                                            >  SRR4302533.6328584/1‑94 (MQ=60)
                                    GGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGCTCTCTATCCACGCG                                                           >  SRR4302533.1269033/1‑94 (MQ=60)
                                     GCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGG                                                          <  SRR4302533.1698476/94‑1 (MQ=60)
                                      GGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGC                                                         <  SRR4302533.3015595/94‑1 (MQ=60)
                                      CGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGC                                                         <  SRR4302533.4379298/94‑1 (MQ=60)
                                       GGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCG                                                        >  SRR4302533.2599692/1‑94 (MQ=60)
                                        GAATGGCGGAGTTTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGG                                                       <  SRR4302533.331311/94‑1 (MQ=60)
                                         AATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGT                                                      >  SRR4302533.2434619/1‑94 (MQ=60)
                                         AATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGT                                                      <  SRR4302533.1740134/94‑1 (MQ=60)
                                          ATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTC                                                     <  SRR4302533.6239627/94‑1 (MQ=60)
                                           TGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCA                                                    <  SRR4302533.5157438/94‑1 (MQ=60)
                                           TGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCA                                                    <  SRR4302533.4907867/94‑1 (MQ=60)
                                            GGCGGAGATTGTTTGTTTTAATGCCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGTTGGTTCGCGATCTCTATCCACGCGGCGGTCAT                                                   <  SRR4302533.5334727/94‑1 (MQ=60)
                                              CGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGT                                                 >  SRR4302533.5379719/1‑94 (MQ=60)
                                               GGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTC                                                <  SRR4302533.4880090/94‑1 (MQ=60)
                                               GGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGCGGTTCGCGATCTCTATCCACGCGGCGGTCATGTC                                                >  SRR4302533.3797674/1‑94 (MQ=60)
                                                GATATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCG                                               <  SRR4302533.4660988/94‑1 (MQ=60)
                                                GAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCG                                               >  SRR4302533.3006170/1‑94 (MQ=60)
                                                GAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCG                                               <  SRR4302533.4702399/94‑1 (MQ=60)
                                                 AGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGA                                              >  SRR4302533.5357613/1‑94 (MQ=60)
                                                  GATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAG                                             <  SRR4302533.1310157/94‑1 (MQ=60)
                                                     TGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAG                                          <  SRR4302533.5075887/94‑1 (MQ=60)
                                                      GTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGG                                         >  SRR4302533.5094949/1‑94 (MQ=60)
                                                      GTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGG                                         <  SRR4302533.5802226/94‑1 (MQ=60)
                                                         GGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGA                                      <  SRR4302533.4738220/94‑1 (MQ=60)
                                                         GGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGA                                      >  SRR4302533.2597288/1‑94 (MQ=60)
                                                          GTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGAC                                     >  SRR4302533.4679530/1‑94 (MQ=60)
                                                             TTAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTT                                  >  SRR4302533.3858824/1‑94 (MQ=60)
                                                              TAATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTC                                 <  SRR4302533.3826680/94‑1 (MQ=60)
                                                                ATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTG                               <  SRR4302533.4785688/94‑1 (MQ=60)
                                                                ATACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGACATGTCGAGCAGGCGAGTTTCTG                               >  SRR4302533.3464875/1‑94 (MQ=60)
                                                                 TACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGG                              >  SRR4302533.3105999/1‑94 (MQ=60)
                                                                 TACCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGG                              >  SRR4302533.1518859/1‑94 (MQ=60)
                                                                   CCGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGAC                            >  SRR4302533.106577/1‑94 (MQ=60)
                                                                    CGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACT                           <  SRR4302533.1325335/94‑1 (MQ=60)
                                                                    CGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACT                           >  SRR4302533.5884946/1‑94 (MQ=60)
                                                                    CGAGCTGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACT                           >  SRR4302533.4328356/1‑94 (MQ=60)
                                                                         TGTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCT                      >  SRR4302533.97036/1‑94 (MQ=60)
                                                                          GTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTG                     <  SRR4302533.4389926/94‑1 (MQ=60)
                                                                          GTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTG                     <  SRR4302533.2286453/94‑1 (MQ=60)
                                                                          GTTGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTC                     >  SRR4302533.4122485/1‑94 (MQ=60)
                                                                            TGCAACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCG                   <  SRR4302533.886105/94‑1 (MQ=60)
                                                                               AACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGGCC                >  SRR4302533.1253304/1‑94 (MQ=60)
                                                                               AACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCC                <  SRR4302533.5654411/94‑1 (MQ=60)
                                                                               AACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCC                >  SRR4302533.1544450/1‑94 (MQ=60)
                                                                               AACCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCC                >  SRR4302533.5770239/1‑94 (MQ=60)
                                                                                 CCGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGA              >  SRR4302533.1523126/1‑94 (MQ=60)
                                                                                  CGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGAT             >  SRR4302533.5688802/1‑94 (MQ=60)
                                                                                  CGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGAT             <  SRR4302533.2833703/94‑1 (MQ=60)
                                                                                  CGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGAT             >  SRR4302533.4680191/1‑94 (MQ=60)
                                                                                  CGCGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGAG             >  SRR4302533.5302487/1‑94 (MQ=60)
                                                                                    CGTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGC           >  SRR4302533.2071087/1‑94 (MQ=60)
                                                                                     GTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGAC          >  SRR4302533.4108195/1‑94 (MQ=60)
                                                                                     GTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGAC          <  SRR4302533.6041900/94‑1 (MQ=60)
                                                                                     GTCGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGAC          >  SRR4302533.5356611/1‑94 (MQ=60)
                                                                                       CGTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGC        >  SRR4302533.4911307/1‑94 (MQ=60)
                                                                                        GTGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCC       >  SRR4302533.4187418/1‑94 (MQ=60)
                                                                                         TGAAGCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCCA      >  SRR4302533.3361206/1‑94 (MQ=60)
                                                                                             GCGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCCAGACG  >  SRR4302533.6061603/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATCGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGCGGAATGGCGGAGATTGTTGGTTTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCCAGACG  >  NC_000913/1237688‑1237874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑