Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,230,972 A→G G188G (GGA→GGG)  umuC → UmuC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,230,9720AG100.0% 720.6 / NA 225G188G (GGA→GGG) umuCUmuC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (118/107);  total (118/107)

Only 100 of 238 total aligned reads displayed.

GCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTT  >  NC_000913/1230882‑1231061
                                                                                          |                                                                                         
gCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGcg                                                                                         >  2:4886703/1‑93 (MQ=255)
gCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGcg                                                                                         <  1:991232/93‑1 (MQ=255)
 cAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGcgg                                                                                        <  2:3996690/93‑1 (MQ=255)
  aGACGGGTGTGGTGGTCGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTTTCCCCGTGGATGACGTCTGGGGGATTGggcggc                                                                                       <  2:3196150/93‑1 (MQ=255)
  aGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGggcggc                                                                                       <  2:1556703/93‑1 (MQ=255)
   gACGGGTGGGGGGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGgcggcg                                                                                      >  1:2460771/1‑93 (MQ=255)
      gggtggggtgGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGAt                                                                                   <  1:2304473/93‑1 (MQ=255)
        gtggggtggTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCa                                                                                 <  1:45839/93‑1 (MQ=255)
        gtggggtggTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCa                                                                                 <  1:4279337/93‑1 (MQ=255)
         tggggtggTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAg                                                                                <  2:2137807/93‑1 (MQ=255)
          ggggtgtTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGc                                                                               <  1:1135720/93‑1 (MQ=255)
          ggggtggTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGc                                                                               >  1:2051720/1‑93 (MQ=255)
             gtggtgGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCaaa                                                                            <  1:4834604/93‑1 (MQ=255)
               ggtggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCaaaaa                                                                          >  1:2004838/1‑93 (MQ=255)
               ggtggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCaaaaa                                                                          >  1:4027057/1‑93 (MQ=255)
               ggtggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCaaaaa                                                                          >  2:327571/1‑93 (MQ=255)
               ggcggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCaaaaa                                                                          >  1:4610002/4‑93 (MQ=255)
                 tggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAAc                                                                        <  2:779627/93‑1 (MQ=255)
                 tggATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAAc                                                                        >  1:2768417/1‑93 (MQ=255)
                   gATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTg                                                                      >  2:3473678/1‑93 (MQ=255)
                   gATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTg                                                                      <  1:4974997/93‑1 (MQ=255)
                   gATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTg                                                                      <  2:3594301/93‑1 (MQ=255)
                   gATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTg                                                                      >  1:135419/1‑93 (MQ=255)
                    aTTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTgg                                                                     <  2:691945/93‑1 (MQ=255)
                     tttATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGa                                                                    <  2:2230735/93‑1 (MQ=255)
                      ttATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGAc                                                                   <  1:1981159/93‑1 (MQ=255)
                        aTCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGAcgc                                                                 >  2:2702820/1‑93 (MQ=255)
                        aTCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGAcgc                                                                 <  2:1373174/93‑1 (MQ=255)
                         tCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGAcgcg                                                                >  1:5364892/1‑93 (MQ=255)
                           aaaTCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGAt                                                              <  2:2525232/93‑1 (MQ=255)
                           aaaTCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGAt                                                              <  2:933874/93‑1 (MQ=255)
                            aaTCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATg                                                             >  2:498026/1‑93 (MQ=255)
                             aTCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATgg                                                            <  1:1644970/93‑1 (MQ=255)
                             aTCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATgg                                                            <  2:1643536/93‑1 (MQ=255)
                               cTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATgggg                                                          <  2:3272392/93‑1 (MQ=255)
                                tGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGa                                                         >  2:3029754/1‑93 (MQ=255)
                                tGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGa                                                         >  1:3021314/1‑93 (MQ=255)
                                tGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGa                                                         >  1:5583290/1‑93 (MQ=255)
                                 ggAACGCCAGCTTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGAt                                                        <  2:5592414/93‑1 (MQ=255)
                                 ggAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGAt                                                        >  1:1437306/1‑93 (MQ=255)
                                 ggAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGAt                                                        <  2:5362/93‑1 (MQ=255)
                                  gAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATc                                                       <  1:2170677/93‑1 (MQ=255)
                                      gCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCaaaa                                                   <  2:1460187/93‑1 (MQ=255)
                                         aGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCg                                                >  2:1891390/1‑93 (MQ=255)
                                          gCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGt                                               >  2:4058569/1‑93 (MQ=255)
                                           cGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGtt                                              <  2:1097653/93‑1 (MQ=255)
                                             tAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTtct                                            >  1:1073422/1‑93 (MQ=255)
                                             tAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTtct                                            >  2:4671194/1‑93 (MQ=255)
                                              aaaTTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTtctc                                           <  2:1530748/93‑1 (MQ=255)
                                                aTTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGa                                         <  1:1568301/93‑1 (MQ=255)
                                                 ttAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGAt                                        <  1:5288462/93‑1 (MQ=255)
                                                  tAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGAtt                                       <  2:5328576/93‑1 (MQ=255)
                                                   aaTGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGAttt                                      <  2:4925813/93‑1 (MQ=255)
                                                   aaTGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGAttt                                      <  1:847831/93‑1 (MQ=255)
                                                    aTGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTg                                     <  1:2650946/93‑1 (MQ=255)
                                                     tGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGAttttg                                    >  2:6081481/1‑91 (MQ=255)
                                                     tGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTgg                                    <  1:649265/93‑1 (MQ=255)
                                                     tGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTgg                                    >  2:6169843/1‑93 (MQ=255)
                                                      gTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGc                                   <  2:2598573/93‑1 (MQ=255)
                                                      gTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGc                                   >  2:4173837/1‑93 (MQ=255)
                                                        cTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCgg                                 >  1:2856167/1‑93 (MQ=255)
                                                         tGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGa                                >  2:1354292/1‑93 (MQ=255)
                                                         tGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGa                                >  1:1811439/1‑93 (MQ=255)
                                                          gcgctcCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGAt                               <  2:3941684/90‑1 (MQ=255)
                                                          gCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGAt                               >  2:6013835/1‑93 (MQ=255)
                                                          gCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGAt                               <  1:2678518/93‑1 (MQ=255)
                                                           ctctCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATa                              <  1:4674626/93‑1 (MQ=255)
                                                           ctctCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATa                              >  2:1699073/1‑93 (MQ=255)
                                                               ccccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGa                          <  1:5633373/93‑1 (MQ=255)
                                                               ccccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGa                          <  1:4372741/93‑1 (MQ=255)
                                                                 ccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGata                        <  2:4402717/93‑1 (MQ=255)
                                                                 ccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGata                        <  1:3508679/93‑1 (MQ=255)
                                                                 ccGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGata                        >  1:6200807/1‑93 (MQ=255)
                                                                  cGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGatat                       >  2:4337274/1‑93 (MQ=255)
                                                                  cGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGatat                       >  1:4523828/1‑93 (MQ=255)
                                                                   gTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATc                      >  1:2607665/1‑93 (MQ=255)
                                                                   gTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCAATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATc                      <  1:3385989/93‑1 (MQ=255)
                                                                    tGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATcc                     >  2:2333341/1‑93 (MQ=255)
                                                                     ggATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCg                    >  1:2211539/1‑93 (MQ=255)
                                                                         gACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGttt                >  1:3524477/1‑93 (MQ=255)
                                                                         gACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGttt                >  1:5182557/1‑93 (MQ=255)
                                                                          aCGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTa               <  2:1169406/93‑1 (MQ=255)
                                                                           cGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTAt              >  2:3146126/1‑93 (MQ=255)
                                                                            gTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATc             <  1:4261252/93‑1 (MQ=255)
                                                                            gTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATc             <  2:4718803/93‑1 (MQ=255)
                                                                             tCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATcc            <  2:1220322/93‑1 (MQ=255)
                                                                             tCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATcc            >  2:5107349/1‑93 (MQ=255)
                                                                             tCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATcc            >  1:5692883/1‑93 (MQ=255)
                                                                             tCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATcc            >  1:3194309/1‑93 (MQ=255)
                                                                             tCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATcc            <  1:3647749/93‑1 (MQ=255)
                                                                             tCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATcc            >  1:3957683/1‑93 (MQ=255)
                                                                              cTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCg           <  1:201650/93‑1 (MQ=255)
                                                                               tGGGGGGTTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGt          >  1:2353355/1‑93 (MQ=255)
                                                                               tGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGt          <  1:5870356/93‑1 (MQ=255)
                                                                                gggggATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTa         >  2:4555127/1‑93 (MQ=255)
                                                                                gggggATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTa         >  1:5004114/1‑93 (MQ=255)
                                                                                    gATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACa     <  2:1605382/93‑1 (MQ=255)
                                                                                      ttGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACAtt   >  2:4473647/1‑93 (MQ=255)
                                                                                      ttGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACAtt   <  2:3687953/93‑1 (MQ=255)
                                                                                       tGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACAttt  <  1:6074586/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTT  >  NC_000913/1230882‑1231061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTTTAAT  >  NC_000913/1230879‑1231065
                                                                                             |                                                                                             
GCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGG                                                                                               <  SRR4302533.350663/94‑1 (MQ=60)
  GGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCG                                                                                             >  SRR4302533.1918392/1‑94 (MQ=60)
   GCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGG                                                                                            <  SRR4302533.997028/94‑1 (MQ=60)
     AGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCG                                                                                          >  SRR4302533.1278569/1‑94 (MQ=60)
     AGACGGGTGGGGGGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCG                                                                                          >  SRR4302533.2475956/1‑94 (MQ=60)
      GACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGG                                                                                         >  SRR4302533.405835/1‑94 (MQ=60)
        CGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGAT                                                                                       <  SRR4302533.263117/94‑1 (MQ=60)
         GGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATC                                                                                      <  SRR4302533.2318749/94‑1 (MQ=60)
           GTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAG                                                                                    <  SRR4302533.46158/94‑1 (MQ=60)
           GTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAG                                                                                    <  SRR4302533.5304384/94‑1 (MQ=60)
           GTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAG                                                                                    <  SRR4302533.4305070/94‑1 (MQ=60)
            TGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGC                                                                                   >  SRR4302533.2064381/1‑94 (MQ=60)
             GGGGTGTTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCA                                                                                  <  SRR4302533.1142474/94‑1 (MQ=60)
               GGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAA                                                                                <  SRR4302533.6204521/94‑1 (MQ=60)
                GTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAA                                                                               <  SRR4302533.4863905/94‑1 (MQ=60)
                GTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAA                                                                               >  SRR4302533.3788659/1‑94 (MQ=60)
                 TGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAA                                                                              >  SRR4302533.4051571/1‑94 (MQ=60)
                 TGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAA                                                                              >  SRR4302533.2017186/1‑94 (MQ=60)
                 TGGCGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAA                                                                              >  SRR4302533.4637921/1‑94 (MQ=60)
                  GGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAA                                                                             >  SRR4302533.1736366/1‑94 (MQ=60)
                  GGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAA                                                                             >  SRR4302533.5734426/1‑94 (MQ=60)
                   GTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAAC                                                                            >  SRR4302533.2785402/1‑94 (MQ=60)
                    TGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACT                                                                           >  SRR4302533.3366933/1‑94 (MQ=60)
                     GGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTG                                                                          >  SRR4302533.2706359/1‑94 (MQ=60)
                     GGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTG                                                                          <  SRR4302533.6266120/94‑1 (MQ=60)
                     GGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTG                                                                          >  SRR4302533.2184481/1‑94 (MQ=60)
                     GGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTG                                                                          >  SRR4302533.136277/1‑94 (MQ=60)
                      GATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGG                                                                         >  SRR4302533.5212196/1‑94 (MQ=60)
                      GATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGG                                                                         <  SRR4302533.5005161/94‑1 (MQ=60)
                      GATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGG                                                                         <  SRR4302533.2873105/94‑1 (MQ=60)
                         TTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACG                                                                      <  SRR4302533.216861/94‑1 (MQ=60)
                         TTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACG                                                                      >  SRR4302533.3985115/1‑94 (MQ=60)
                         TTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACG                                                                      <  SRR4302533.1993287/94‑1 (MQ=60)
                         TTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACG                                                                      >  SRR4302533.5557991/1‑94 (MQ=60)
                           ATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCG                                                                    >  SRR4302533.5397120/1‑94 (MQ=60)
                            TCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGA                                                                   >  SRR4302533.5647257/1‑94 (MQ=60)
                              AAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATG                                                                 <  SRR4302533.2254872/94‑1 (MQ=60)
                               AATCTGGACCGCCAGCGTAAATTAATGTCTGCGCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAACCTGGACGCGATGG                                                                <  SRR4302533.4426603/94‑1 (MQ=60)
                               AATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGCCGCGATGG                                                                >  SRR4302533.4963094/1‑94 (MQ=60)
                               AATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGG                                                                <  SRR4302533.5850050/94‑1 (MQ=60)
                                ATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGG                                                               >  SRR4302533.4166003/1‑94 (MQ=60)
                                ATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGG                                                               <  SRR4302533.1654930/94‑1 (MQ=60)
                                  CTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGA                                                             <  SRR4302533.447435/94‑1 (MQ=60)
                                  CTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGA                                                             >  SRR4302533.5617026/1‑94 (MQ=60)
                                  CTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGA                                                             >  SRR4302533.3039802/1‑94 (MQ=60)
                                   TGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGAT                                                            >  SRR4302533.2025380/1‑94 (MQ=60)
                                   TGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGAT                                                            >  SRR4302533.4884763/1‑94 (MQ=60)
                                   TGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGAT                                                            >  SRR4302533.1445984/1‑94 (MQ=60)
                                     GAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCA                                                          <  SRR4302533.2184027/94‑1 (MQ=60)
                                      AACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAA                                                         >  SRR4302533.1580030/1‑94 (MQ=60)
                                      AACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAA                                                         >  SRR4302533.653029/1‑94 (MQ=60)
                                        CGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGGTCCGCAAAAAACTGGACGCGATGGGGATCAAAC                                                       >  SRR4302533.1106243/1‑94 (MQ=60)
                                        CGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAA                                                       >  SRR4302533.3210674/1‑94 (MQ=60)
                                          CCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACC                                                     >  SRR4302533.849741/1‑94 (MQ=60)
                                           CAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCG                                                    >  SRR4302533.1423386/1‑94 (MQ=60)
                                             GCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTT                                                  <  SRR4302533.2511818/94‑1 (MQ=60)
                                             GCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTT                                                  >  SRR4302533.2740566/1‑94 (MQ=60)
                                               GTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCT                                                >  SRR4302533.1079750/1‑94 (MQ=60)
                                                TAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTC                                               >  SRR4302533.2601117/1‑94 (MQ=60)
                                                 AAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCG                                              >  SRR4302533.3490797/1‑94 (MQ=60)
                                                 AAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCG                                              >  SRR4302533.5700202/1‑94 (MQ=60)
                                                  AATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGA                                             >  SRR4302533.3163936/1‑94 (MQ=60)
                                                   ATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGAT                                            <  SRR4302533.1577743/94‑1 (MQ=60)
                                                    TTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATT                                           <  SRR4302533.5990849/94‑1 (MQ=60)
                                                    TTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATT                                           <  SRR4302533.5320134/94‑1 (MQ=60)
                                                    TTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATT                                           >  SRR4302533.4675871/1‑94 (MQ=60)
                                                     TAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTT                                          >  SRR4302533.1731159/1‑94 (MQ=60)
                                                      AATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTG                                         <  SRR4302533.3035553/94‑1 (MQ=60)
                                                      AATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTG                                         <  SRR4302533.852792/94‑1 (MQ=60)
                                                      AATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTG                                         <  SRR4302533.35373/94‑1 (MQ=60)
                                                       ATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGG                                        <  SRR4302533.3479863/94‑1 (MQ=60)
                                                       ATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGG                                        <  SRR4302533.2667229/94‑1 (MQ=60)
                                                       ATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGG                                        >  SRR4302533.5011712/1‑94 (MQ=60)
                                                        TGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGC                                       <  SRR4302533.653096/94‑1 (MQ=60)
                                                         GTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCG                                      <  SRR4302533.2002686/94‑1 (MQ=60)
                                                          TCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGG                                     >  SRR4302533.2873742/1‑94 (MQ=60)
                                                           CTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGA                                    >  SRR4302533.1822505/1‑94 (MQ=60)
                                                            TGCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGAT                                   <  SRR4302533.4291195/94‑1 (MQ=60)
                                                             GCTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATA                                  <  SRR4302533.2694935/94‑1 (MQ=60)
                                                              CTCTCCGCGTGGATGACGTCTGGGGGATGGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATAC                                 <  SRR4302533.4666768/94‑1 (MQ=60)
                                                              CTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATAC                                 >  SRR4302533.858771/1‑94 (MQ=60)
                                                              CTCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATAC                                 <  SRR4302533.4702951/94‑1 (MQ=60)
                                                               TCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACA                                <  SRR4302533.2362873/94‑1 (MQ=60)
                                                               CCTCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACA                                <  SRR4302533.2423478/94‑1 (MQ=60)
                                                                 TCCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGA                              <  SRR4302533.2682158/94‑1 (MQ=60)
                                                                  CCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGAT                             <  SRR4302533.4399135/94‑1 (MQ=60)
                                                                  CCCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGAT                             <  SRR4302533.5667439/94‑1 (MQ=60)
                                                                   CCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATA                            <  SRR4302533.2039632/94‑1 (MQ=60)
                                                                   CCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATA                            >  SRR4302533.6237999/1‑94 (MQ=60)
                                                                   CCCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATA                            >  SRR4302533.3633151/1‑94 (MQ=60)
                                                                    CCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATAT                           <  SRR4302533.3530312/94‑1 (MQ=60)
                                                                    CCGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATAT                           >  SRR4302533.4551214/1‑94 (MQ=60)
                                                                     CGTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATC                          >  SRR4302533.2623679/1‑94 (MQ=60)
                                                                      GTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCC                         <  SRR4302533.4330420/94‑1 (MQ=60)
                                                                      GTGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCAATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCC                         <  SRR4302533.3406896/94‑1 (MQ=60)
                                                                       TGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCG                        >  SRR4302533.1573820/1‑94 (MQ=60)
                                                                       TGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCG                        <  SRR4302533.5625180/94‑1 (MQ=60)
                                                                       TGGATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCG                        >  SRR4302533.2225176/1‑94 (MQ=60)
                                                                          ATGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTT                     <  SRR4302533.1384012/94‑1 (MQ=60)
                                                                           TGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTT                    >  SRR4302533.3546198/1‑94 (MQ=60)
                                                                           TGACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTT                    >  SRR4302533.5213849/1‑94 (MQ=60)
                                                                            GACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGGTTA                   >  SRR4302533.5337953/1‑94 (MQ=60)
                                                                             ACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTAT                  >  SRR4302533.875966/1‑94 (MQ=60)
                                                                             ACGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTAT                  >  SRR4302533.2350893/1‑94 (MQ=60)
                                                                              CGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATC                 <  SRR4302533.526113/94‑1 (MQ=60)
                                                                              CGTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATC                 >  SRR4302533.3861941/1‑94 (MQ=60)
                                                                               GTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCC                >  SRR4302533.3981803/1‑94 (MQ=60)
                                                                               GTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCC                >  SRR4302533.3213961/1‑94 (MQ=60)
                                                                               GTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCC                >  SRR4302533.5727263/1‑94 (MQ=60)
                                                                               GTCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCC                <  SRR4302533.4286929/94‑1 (MQ=60)
                                                                                TCTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCG               <  SRR4302533.3670176/94‑1 (MQ=60)
                                                                                 CTGGGGGGTTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGT              >  SRR4302533.2367910/1‑94 (MQ=60)
                                                                                 CTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGT              >  SRR4302533.4620053/1‑94 (MQ=60)
                                                                                 CTGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGT              <  SRR4302533.202930/94‑1 (MQ=60)
                                                                                  TGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTA             <  SRR4302533.5905838/94‑1 (MQ=60)
                                                                                  TGGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTA             >  SRR4302533.5034441/1‑94 (MQ=60)
                                                                                   GGGGGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAA            <  SRR4302533.483633/94‑1 (MQ=60)
                                                                                      GGATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACA         >  SRR4302533.4601743/1‑94 (MQ=60)
                                                                                        ATTGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATT       <  SRR4302533.2005853/94‑1 (MQ=60)
                                                                                          TGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTTT     >  SRR4302533.2308460/1‑94 (MQ=60)
                                                                                          TGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTTT     <  SRR4302533.6111330/94‑1 (MQ=60)
                                                                                          TGGGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTTT     <  SRR4302533.2084968/94‑1 (MQ=60)
                                                                                            GGCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTTTAA   >  SRR4302533.620486/1‑94 (MQ=60)
                                                                                             GCGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTTTAAT  <  SRR4302533.1802855/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCGGCAGACGGGTGGGGTGGTGGATTTATCAAATCTGGAACGCCAGCGTAAATTAATGTCTGCTCTCCCCGTGGATGACGTCTGGGGGATTGGACGGCGGATCAGCAAAAAACTGGACGCGATGGGGATCAAAACCGTTCTCGATTTGGCGGATACAGATATCCGGTTTATCCGTAAACATTTTAAT  >  NC_000913/1230879‑1231065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑