Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,289,543 T→C F27L (TTT→CTT)  hnr → Hnr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,289,5430TC100.0% 737.7 / NA 228F27L (TTT→CTT) hnrHnr
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (123/105);  total (123/105)

Only 100 of 235 total aligned reads displayed.

AGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATA  >  NC_000913/1289453‑1289632
                                                                                          |                                                                                         
 ggggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGcttt                                                                                        <  1:3558627/93‑1 (MQ=255)
   ggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTcc                                                                                      >  1:4877260/1‑93 (MQ=255)
   ggAGAGAACAGGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGTATTCATGGCTTTcc                                                                                      <  1:1788936/93‑1 (MQ=255)
       agaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCAt                                                                                  <  2:3080687/93‑1 (MQ=255)
        gaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCAtt                                                                                 <  1:1202120/93‑1 (MQ=255)
        gaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCAtt                                                                                 <  2:5351495/93‑1 (MQ=255)
         aaCATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTg                                                                                <  1:4652071/93‑1 (MQ=255)
          aCATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCTCTCGCTTCTGGATTCATGGCTTTCCTCAtttg                                                                               >  2:4217559/1‑91 (MQ=255)
          aCATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTgg                                                                               <  2:4475925/93‑1 (MQ=255)
          aCATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTgg                                                                               >  1:5359415/1‑93 (MQ=255)
            aTGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGa                                                                             <  1:1496661/93‑1 (MQ=255)
            aTGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGa                                                                             >  2:1541552/1‑93 (MQ=255)
            aTGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGa                                                                             <  2:2962120/93‑1 (MQ=255)
               aCGCAGCCATTGGTCGTAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCg                                                                          <  2:5876854/93‑1 (MQ=255)
               aCGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCg                                                                          >  1:3821792/1‑93 (MQ=255)
               aCGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATCGGGAGCg                                                                          <  2:2255107/93‑1 (MQ=255)
                cGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGa                                                                         >  2:1417372/1‑93 (MQ=255)
                   aGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACaa                                                                      <  1:3382574/93‑1 (MQ=255)
                    gCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAAc                                                                     >  2:2693111/1‑93 (MQ=255)
                     ccATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACg                                                                    <  1:3513436/93‑1 (MQ=255)
                      cATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACgg                                                                   <  2:5672607/93‑1 (MQ=255)
                       aTTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGt                                                                  <  2:1375308/93‑1 (MQ=255)
                        ttGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTa                                                                 >  2:1122114/1‑93 (MQ=255)
                         tGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTAc                                                                <  2:5537561/93‑1 (MQ=255)
                         tGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTAc                                                                <  1:1640518/93‑1 (MQ=255)
                          ggTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACt                                                               <  1:5421197/93‑1 (MQ=255)
                           gTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTg                                                              <  2:3119466/93‑1 (MQ=255)
                           gTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTg                                                              <  2:4550823/93‑1 (MQ=255)
                           gTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTg                                                              <  2:3629555/93‑1 (MQ=255)
                             cGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggc                                                            <  1:4681190/93‑1 (MQ=255)
                             cGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggc                                                            >  1:5247859/1‑93 (MQ=255)
                              ggAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggcg                                                           >  1:4397246/1‑93 (MQ=255)
                              ggAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggcg                                                           >  2:4069726/1‑93 (MQ=255)
                               gAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggcgg                                                          <  2:3740559/93‑1 (MQ=255)
                               gAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggcgg                                                          >  2:3583943/1‑93 (MQ=255)
                                 aaaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCt                                                        >  1:4057305/1‑93 (MQ=255)
                                 aaaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCt                                                        >  1:5512550/1‑93 (MQ=255)
                                 aaaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCt                                                        >  1:5853574/1‑93 (MQ=255)
                                  aaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTg                                                       >  2:5634659/1‑93 (MQ=255)
                                  aaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTg                                                       >  2:3499592/1‑93 (MQ=255)
                                   aCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGa                                                      <  1:2705698/93‑1 (MQ=255)
                                    cAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGAt                                                     <  2:4662130/93‑1 (MQ=255)
                                    cAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGAt                                                     <  2:1665509/93‑1 (MQ=255)
                                    cAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGAt                                                     <  1:1236378/93‑1 (MQ=255)
                                      gATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgg                                                   >  1:3120330/1‑93 (MQ=255)
                                      gATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgg                                                   >  2:863839/1‑93 (MQ=255)
                                         tctcATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATggggg                                                >  1:17602/1‑92 (MQ=255)
                                         tctcATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGt                                                <  2:5268112/93‑1 (MQ=255)
                                         tctcATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGt                                                <  2:1077392/93‑1 (MQ=255)
                                          ctcATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTg                                               >  2:5400591/1‑93 (MQ=255)
                                          ctcATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTg                                               <  1:2081714/93‑1 (MQ=255)
                                           tcATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTgg                                              >  2:1201609/1‑93 (MQ=255)
                                           tcATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTgg                                              >  2:5905439/1‑93 (MQ=255)
                                           tcATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTgg                                              >  2:1004218/1‑93 (MQ=255)
                                            cATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGa                                             <  1:4584191/93‑1 (MQ=255)
                                             aTTTTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGAt                                            <  2:913282/93‑1 (MQ=255)
                                              ttgttgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATg                                           >  1:443323/1‑93 (MQ=255)
                                                gttgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGcc                                         >  1:1448472/1‑93 (MQ=255)
                                                 ttgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGgcc                                        >  1:5080980/1‑93 (MQ=255)
                                                  tgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCt                                       <  1:1132617/93‑1 (MQ=255)
                                                    aaGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTg                                     >  2:431882/1‑93 (MQ=255)
                                                    aaGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTg                                     <  2:3886779/93‑1 (MQ=255)
                                                     aGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGa                                    >  1:5816601/1‑93 (MQ=255)
                                                      gATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGGTGGGGGGGATGCCCTTGAg                                   >  2:2360283/1‑93 (MQ=255)
                                                        tGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGtt                                 >  1:624152/1‑93 (MQ=255)
                                                        tGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGtt                                 <  1:2681796/93‑1 (MQ=255)
                                                          aGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGc                               >  1:1367325/1‑93 (MQ=255)
                                                          aGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGc                               <  2:5791260/93‑1 (MQ=255)
                                                          aGCAGGTATTTCGCTCGCTTCTGAATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGc                               <  2:4920863/93‑1 (MQ=255)
                                                            cAGGTATTTCGCTCGCTGCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTg                             <  2:574931/93‑1 (MQ=255)
                                                             aGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTgg                            <  2:2755203/93‑1 (MQ=255)
                                                                tATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGGTGCCCTTGAGTTGCTGGGAg                         >  1:4208110/1‑93 (MQ=255)
                                                                  tttCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGt                       <  2:3176365/93‑1 (MQ=255)
                                                                  tttCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGt                       >  1:6171963/1‑93 (MQ=255)
                                                                     cgctcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTc                    >  2:2002159/1‑93 (MQ=255)
                                                                      gctcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGtttga                   >  1:1859167/1‑91 (MQ=255)
                                                                      gctcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGGTTCa                   >  2:2600514/1‑93 (MQ=255)
                                                                        tcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGGTGGTGTGGTTGGTGCGCTTGAGTTGCTGGGCGGTTTCGCt                 >  1:2756861/1‑93 (MQ=255)
                                                                        tcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACt                 >  1:4369733/1‑93 (MQ=255)
                                                                          gctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTcc               >  1:898935/1‑93 (MQ=255)
                                                                          gctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGGTTCACTcc               >  2:4770281/1‑93 (MQ=255)
                                                                           ctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGCGGTTTCACTCCa              >  1:2630750/1‑93 (MQ=255)
                                                                           ctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCa              >  1:5970683/1‑93 (MQ=255)
                                                                           ctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCa              >  2:3204141/1‑93 (MQ=255)
                                                                           ctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGGTTCACTCCa              >  1:181189/1‑93 (MQ=255)
                                                                            ttttGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAg             <  1:4984415/90‑1 (MQ=255)
                                                                            ttCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAg             >  1:1716369/1‑93 (MQ=255)
                                                                             tCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGa            <  2:5008926/93‑1 (MQ=255)
                                                                             tCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGa            <  2:1246108/93‑1 (MQ=255)
                                                                             tCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGGGGATGCCCTTGAGTTGCTGGGAGGGTTGACTCCAGa            >  1:2355319/1‑93 (MQ=255)
                                                                               tGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGAcc          >  1:5638645/1‑93 (MQ=255)
                                                                                ggATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCt         >  2:5741045/1‑93 (MQ=255)
                                                                                ggATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGAACt         >  1:646013/1‑93 (MQ=255)
                                                                                  aTTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCtga       <  2:6276993/93‑1 (MQ=255)
                                                                                   ttCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCtgat      <  2:6136830/93‑1 (MQ=255)
                                                                                    tCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCtgatg     >  1:3756456/1‑93 (MQ=255)
                                                                                      aTGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTgatgat   >  2:680794/1‑93 (MQ=255)
                                                                                       tGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGata  <  2:2132852/93‑1 (MQ=255)
                                                                                       tGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGata  >  2:2044211/1‑93 (MQ=255)
                                                                                       tGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGata  <  2:730453/93‑1 (MQ=255)
                                                                                          |                                                                                         
AGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATA  >  NC_000913/1289453‑1289632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGTG  >  NC_000913/1289450‑1289636
                                                                                             |                                                                                             
GTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGC                                                                                               >  SRR4302533.1226417/1‑94 (MQ=60)
  CAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTT                                                                                             <  SRR4302533.4766093/94‑1 (MQ=60)
   AGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTT                                                                                            >  SRR4302533.3783286/1‑94 (MQ=60)
   AGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTT                                                                                            >  SRR4302533.4703827/1‑94 (MQ=60)
    GGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTC                                                                                           <  SRR4302533.2815703/94‑1 (MQ=60)
    GGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTC                                                                                           <  SRR4302533.3580555/94‑1 (MQ=60)
     GGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCC                                                                                          <  SRR4302533.4603232/94‑1 (MQ=60)
     GGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCC                                                                                          >  SRR4302533.4906809/1‑94 (MQ=60)
      GGAGAGAACAGGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGTATTCATGGCTTTCCT                                                                                         <  SRR4302533.1799816/94‑1 (MQ=60)
         GAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCAT                                                                                      <  SRR4302533.1979109/94‑1 (MQ=60)
           GAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTG                                                                                    >  SRR4302533.6197002/1‑94 (MQ=60)
           GAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTG                                                                                    <  SRR4302533.1209343/94‑1 (MQ=60)
            AACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGG                                                                                   >  SRR4302533.5391598/1‑94 (MQ=60)
            AACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGG                                                                                   <  SRR4302533.4680260/94‑1 (MQ=60)
              CATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGA                                                                                 <  SRR4302533.3151646/94‑1 (MQ=60)
               ATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAG                                                                                <  SRR4302533.4233635/94‑1 (MQ=60)
               ATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAG                                                                                <  SRR4302533.1505675/94‑1 (MQ=60)
                TGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGC                                                                               <  SRR4302533.3537454/94‑1 (MQ=60)
                 GACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCG                                                                              >  SRR4302533.3845160/1‑94 (MQ=60)
                    GCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACA                                                                           >  SRR4302533.5267650/1‑94 (MQ=60)
                      AGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAAC                                                                         <  SRR4302533.3403454/94‑1 (MQ=60)
                       GCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACG                                                                        <  SRR4302533.257725/94‑1 (MQ=60)
                       GCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACG                                                                        >  SRR4302533.3815638/1‑94 (MQ=60)
                        CCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGG                                                                       <  SRR4302533.3535095/94‑1 (MQ=60)
                        CCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGG                                                                       <  SRR4302533.4455539/94‑1 (MQ=60)
                         CATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGT                                                                      <  SRR4302533.2007553/94‑1 (MQ=60)
                            TGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACT                                                                   <  SRR4302533.1650455/94‑1 (MQ=60)
                             GGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTG                                                                  <  SRR4302533.5453818/94‑1 (MQ=60)
                               TCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGC                                                                >  SRR4302533.5279407/1‑94 (MQ=60)
                               TCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGC                                                                >  SRR4302533.2745690/1‑94 (MQ=60)
                                CGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCG                                                               >  SRR4302533.4423794/1‑94 (MQ=60)
                                CGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCG                                                               <  SRR4302533.4709555/94‑1 (MQ=60)
                                CGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCG                                                               <  SRR4302533.3481838/94‑1 (MQ=60)
                                CGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATGTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCG                                                               <  SRR4302533.5719486/94‑1 (MQ=60)
                                 GGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGG                                                              >  SRR4302533.5148479/1‑94 (MQ=60)
                                   AAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCT                                                            >  SRR4302533.4082003/1‑94 (MQ=60)
                                   AAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCT                                                            >  SRR4302533.5545827/1‑94 (MQ=60)
                                   AAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCT                                                            >  SRR4302533.5888963/1‑94 (MQ=60)
                                     AACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGA                                                          >  SRR4302533.1342177/1‑94 (MQ=60)
                                      ACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGAT                                                         <  SRR4302533.2722292/94‑1 (MQ=60)
                                       CAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATG                                                        <  SRR4302533.1243766/94‑1 (MQ=60)
                                        AGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGG                                                       <  SRR4302533.960732/94‑1 (MQ=60)
                                        AGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGG                                                       >  SRR4302533.3139520/1‑94 (MQ=60)
                                           TTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGT                                                    <  SRR4302533.1237177/94‑1 (MQ=60)
                                           TTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGT                                                    <  SRR4302533.3926029/94‑1 (MQ=60)
                                           TTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGG                                                    >  SRR4302533.17723/1‑94 (MQ=60)
                                           TTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGG                                                    >  SRR4302533.5874469/1‑94 (MQ=60)
                                            TCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTG                                                   >  SRR4302533.1628571/1‑94 (MQ=60)
                                            TCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTG                                                   <  SRR4302533.4611916/94‑1 (MQ=60)
                                             CTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGG                                                  <  SRR4302533.2094525/94‑1 (MQ=60)
                                               CATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGAT                                                <  SRR4302533.4611962/94‑1 (MQ=60)
                                                ATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATG                                               >  SRR4302533.445973/1‑94 (MQ=60)
                                                 TTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGC                                              >  SRR4302533.623124/1‑94 (MQ=60)
                                                  TGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCC                                             >  SRR4302533.1457210/1‑94 (MQ=60)
                                                   GTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGGCC                                            >  SRR4302533.5111686/1‑94 (MQ=60)
                                                   GTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCC                                            <  SRR4302533.5343884/94‑1 (MQ=60)
                                                   GTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCC                                            >  SRR4302533.4785242/1‑94 (MQ=60)
                                                     TGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTT                                          <  SRR4302533.1139351/94‑1 (MQ=60)
                                                      GAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGCGGAGGCCCTTG                                         >  SRR4302533.4392065/1‑94 (MQ=60)
                                                       AAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGA                                        >  SRR4302533.5851732/1‑94 (MQ=60)
                                                        AGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAG                                       <  SRR4302533.1482468/94‑1 (MQ=60)
                                                        AGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAG                                       <  SRR4302533.5071397/94‑1 (MQ=60)
                                                        AGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAG                                       >  SRR4302533.1779251/1‑94 (MQ=60)
                                                        AGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGACCTTGAG                                       >  SRR4302533.5396271/1‑94 (MQ=60)
                                                         GATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGCGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGT                                      <  SRR4302533.3409334/94‑1 (MQ=60)
                                                         GATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGT                                      >  SRR4302533.1308884/1‑94 (MQ=60)
                                                          ATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTT                                     >  SRR4302533.627815/1‑94 (MQ=60)
                                                           TGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTG                                    <  SRR4302533.2103954/94‑1 (MQ=60)
                                                           TGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTG                                    <  SRR4302533.2698230/94‑1 (MQ=60)
                                                            GAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGC                                   >  SRR4302533.765957/1‑94 (MQ=60)
                                                            GAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGC                                   >  SRR4302533.1375545/1‑94 (MQ=60)
                                                            GAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGC                                   >  SRR4302533.5600820/1‑94 (MQ=60)
                                                             AGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCT                                  <  SRR4302533.4756105/94‑1 (MQ=60)
                                                             AGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCT                                  <  SRR4302533.1684773/94‑1 (MQ=60)
                                                             AGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGGGGATGCCCTTTAGTTGCT                                  >  SRR4302533.5501468/1‑94 (MQ=60)
                                                             AGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGGGGATGCCCTTGAGCTGCT                                  >  SRR4302533.161475/1‑94 (MQ=60)
                                                              GCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTG                                 <  SRR4302533.5928323/94‑1 (MQ=60)
                                                              GCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTG                                 >  SRR4302533.1393826/1‑94 (MQ=60)
                                                                  GTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGGTGCCCTTGAGTTGCTGGGAG                             >  SRR4302533.4233570/1‑94 (MQ=60)
                                                                  GTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAG                             <  SRR4302533.4413995/94‑1 (MQ=60)
                                                                  GTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAG                             >  SRR4302533.856441/1‑94 (MQ=60)
                                                                   TATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGCGG                            >  SRR4302533.5011963/1‑94 (MQ=60)
                                                                   TATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGG                            <  SRR4302533.1814498/94‑1 (MQ=60)
                                                                   TATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGCTGCTGGGAGG                            >  SRR4302533.179028/1‑94 (MQ=60)
                                                                    ATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGT                           >  SRR4302533.6209052/1‑94 (MQ=60)
                                                                        CGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTGA                       >  SRR4302533.1870506/1‑94 (MQ=60)
                                                                        CGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCA                       >  SRR4302533.1141992/1‑94 (MQ=60)
                                                                        CGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCA                       >  SRR4302533.6019412/1‑94 (MQ=60)
                                                                         GCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCAC                      >  SRR4302533.416610/1‑94 (MQ=60)
                                                                         GCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCAC                      <  SRR4302533.3766322/94‑1 (MQ=60)
                                                                          CTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGGTGGTGTGGTTGGTGCGCTTGAGTTGCTGGGCGGTTTCGCT                     >  SRR4302533.2773767/1‑94 (MQ=57)
                                                                          CTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACT                     >  SRR4302533.269117/1‑94 (MQ=60)
                                                                          CTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACT                     >  SRR4302533.1838950/1‑94 (MQ=60)
                                                                          CTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACT                     >  SRR4302533.4396116/1‑94 (MQ=60)
                                                                            CGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCC                   >  SRR4302533.904208/1‑94 (MQ=60)
                                                                            CGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGGTTCACTCC                   >  SRR4302533.2706790/1‑94 (MQ=60)
                                                                             GCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGCGGTTTCACTCCA                  >  SRR4302533.2646917/1‑94 (MQ=60)
                                                                             GCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCA                  <  SRR4302533.2675998/94‑1 (MQ=60)
                                                                             GCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCA                  >  SRR4302533.6006828/1‑94 (MQ=60)
                                                                             GCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGGTTCACTCCA                  >  SRR4302533.182318/1‑94 (MQ=60)
                                                                              CTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAG                 >  SRR4302533.1726774/1‑94 (MQ=60)
                                                                              CTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAG                 <  SRR4302533.5850314/94‑1 (MQ=60)
                                                                               TTTTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGA                <  SRR4302533.5014637/94‑1 (MQ=60)
                                                                               TTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGA                >  SRR4302533.4632188/1‑94 (MQ=60)
                                                                               TTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGA                <  SRR4302533.1734182/94‑1 (MQ=60)
                                                                               TTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGGGGATGCCCTTGAGTTGCTGGGAGGGTTGACTCCAGA                >  SRR4302533.2369883/1‑94 (MQ=60)
                                                                               TTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGAGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGC                >  SRR4302533.6328195/1‑94 (MQ=60)
                                                                                 CTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACC              >  SRR4302533.5672735/1‑94 (MQ=60)
                                                                                  TGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGAACT             >  SRR4302533.649819/1‑94 (MQ=60)
                                                                                     ATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGAT          <  SRR4302533.5764981/94‑1 (MQ=60)
                                                                                     ATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGAT          >  SRR4302533.5456944/1‑94 (MQ=60)
                                                                                      TTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATG         >  SRR4302533.3779482/1‑94 (MQ=60)
                                                                                         ATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATA      >  SRR4302533.2931297/1‑94 (MQ=60)
                                                                                           GGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATG    <  SRR4302533.2052539/94‑1 (MQ=60)
                                                                                            GCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGT   <  SRR4302533.4982407/94‑1 (MQ=60)
                                                                                             CTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGTG  <  SRR4302533.4448684/94‑1 (MQ=60)
                                                                                             CTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGTG  >  SRR4302533.5304756/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGTG  >  NC_000913/1289450‑1289636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑