Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,289,543	T→C	F27L (TTT→CTT)	hnr →	Hnr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,289,543	0	T	C	99.6%	834.3 / NA	259	F27L (TTT→CTT)	hnr	Hnr
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (138/120); total (138/121)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

AGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATA  >  NC_000913/1289453‑1289632

  gggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTc                                                                                       >  1:7244673/1‑93 (MQ=255)

  gggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTc                                                                                       >  2:6344038/1‑93 (MQ=255)

  gggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTc                                                                                       >  2:6288916/1‑93 (MQ=255)

   ggAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTcc                                                                                      <  1:2233106/93‑1 (MQ=255)

      gagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGTATTTATGGCTTtcatca                                                                                   >  1:6108907/1‑93 (MQ=255)

      gagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCa                                                                                   <  2:3554818/93‑1 (MQ=255)

      gagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCa                                                                                   >  1:4589558/1‑93 (MQ=255)

      gagaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCa                                                                                   <  2:1780534/93‑1 (MQ=255)

        gaACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCAtt                                                                                 <  2:9101261/93‑1 (MQ=255)

         aaCATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTg                                                                                <  2:8558595/93‑1 (MQ=255)

          aCATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTgg                                                                               <  2:1987455/93‑1 (MQ=255)

           cATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTggg                                                                              >  2:4087768/1‑93 (MQ=255)

           cATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTggg                                                                              <  2:9673399/93‑1 (MQ=255)

             tGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTgggtg                                                                            >  2:7712144/1‑91 (MQ=255)

             tGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAg                                                                            <  2:1302279/93‑1 (MQ=255)

             tGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAg                                                                            <  1:328237/93‑1 (MQ=255)

             tGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAg                                                                            <  1:9374006/93‑1 (MQ=255)

              gACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGc                                                                           <  2:5054417/93‑1 (MQ=255)

                cGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGa                                                                         <  1:6078633/93‑1 (MQ=255)

                  cAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACa                                                                       >  1:1006598/1‑93 (MQ=255)

                  cAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACa                                                                       >  2:1172242/1‑93 (MQ=255)

                   aGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACaa                                                                      <  1:259453/93‑1 (MQ=255)

                   aGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACaa                                                                      >  1:8559925/1‑93 (MQ=255)

                   aGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACaa                                                                      <  1:5989922/93‑1 (MQ=255)

                    gCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAAc                                                                     <  1:8372168/93‑1 (MQ=255)

                    gCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAAc                                                                     >  1:1386562/1‑93 (MQ=255)

                      cATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACgg                                                                   >  2:7287200/1‑93 (MQ=255)

                      cATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACgg                                                                   >  1:1409572/1‑93 (MQ=255)

                      cATGGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACgg                                                                   <  2:2093086/93‑1 (MQ=255)

                       aTTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGt                                                                  >  1:1611833/1‑93 (MQ=255)

                         tGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTAc                                                                <  1:2812407/93‑1 (MQ=255)

                         tGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTAc                                                                <  1:3965191/93‑1 (MQ=255)

                         tGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTAc                                                                >  2:4097275/1‑93 (MQ=255)

                          ggTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACt                                                               <  2:4400562/93‑1 (MQ=255)

                           gTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTg                                                              >  2:2447021/1‑93 (MQ=255)

                           gTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTg                                                              <  2:2588199/93‑1 (MQ=255)

                           gTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTg                                                              >  1:1186534/1‑93 (MQ=255)

                            gcGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTgg                                                             <  2:7521041/92‑1 (MQ=255)

                             cGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggc                                                            <  2:3047340/93‑1 (MQ=255)

                                aaaaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggcggc                                                         <  1:5293113/93‑1 (MQ=255)

                                aaaaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTggcggc                                                         >  1:739179/1‑93 (MQ=255)

                                 aaaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCt                                                        >  2:8812315/1‑93 (MQ=255)

                                  aaCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTg                                                       >  2:1472808/1‑93 (MQ=255)

                                   aCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGa                                                      <  1:2463905/93‑1 (MQ=255)

                                   aCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGa                                                      <  1:2335412/93‑1 (MQ=255)

                                   aCAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGa                                                      >  1:10009240/1‑93 (MQ=255)

                                    cAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGAt                                                     <  2:477569/93‑1 (MQ=255)

                                     aGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATg                                                    >  2:3989455/1‑93 (MQ=255)

                                     aGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATg                                                    >  1:691859/1‑93 (MQ=255)

                                      gATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgg                                                   >  1:6750758/1‑93 (MQ=255)

                                       aTTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATggg                                                  <  1:2131897/93‑1 (MQ=255)

                                        ttCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgggg                                                 <  1:2518063/93‑1 (MQ=255)

                                        ttCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATgggg                                                 <  1:3587372/93‑1 (MQ=255)

                                         tctcATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATggggg                                                >  2:6444617/1‑92 (MQ=255)

                                         tctcATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGt                                                <  2:9484957/93‑1 (MQ=255)

                                            cATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGa                                             <  1:5683123/93‑1 (MQ=255)

                                            cATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGa                                             <  1:6004417/93‑1 (MQ=255)

                                             aTTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGAt                                            <  2:5320902/93‑1 (MQ=255)

                                               tgttgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGGGGATGc                                          >  1:8327069/1‑93 (MQ=255)

                                                gttgAAGATGAGCAGGGATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCGGACGGGGTGGATGcc                                         >  2:8957113/1‑93 (MQ=255)

                                                 ttgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGccc                                        <  1:7409256/93‑1 (MQ=255)

                                                  tgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCt                                       >  2:1551325/1‑93 (MQ=255)

                                                  tgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCt                                       <  2:2972039/93‑1 (MQ=255)

                                                  tgAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCt                                       <  1:6268706/93‑1 (MQ=255)

                                                   gAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCtt                                      >  2:5664805/1‑93 (MQ=255)

                                                   gAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCtt                                      >  1:9206067/1‑93 (MQ=255)

                                                    aaGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTg                                     >  1:288130/1‑93 (MQ=255)

                                                     aGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGa                                    >  2:4368526/1‑93 (MQ=255)

                                                        tGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGtt                                 >  2:3955428/1‑93 (MQ=255)

                                                         gAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTg                                >  2:9443421/1‑93 (MQ=255)

                                                         gAGCAGGTATTTCGCTCGCTGCTGGATTCATGGCTGTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGGCCTTGAGTTg                                <  2:5222159/93‑1 (MQ=255)

                                                            cAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTg                             >  1:4234261/1‑93 (MQ=255)

                                                             aGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTgg                            <  2:3363594/93‑1 (MQ=255)

                                                             aGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTgg                            >  2:7336890/1‑93 (MQ=255)

                                                              ggTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTggg                           >  2:6042607/1‑93 (MQ=255)

                                                               gTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTgggg                          >  2:323475/1‑92 (MQ=255)

                                                               gTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGa                          <  2:9751224/93‑1 (MQ=255)

                                                                tATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAg                         >  2:3290162/1‑93 (MQ=255)

                                                                 aTTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAgg                        >  1:9030770/1‑93 (MQ=255)

                                                                  tttCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGt                       <  2:3788243/93‑1 (MQ=255)

                                                                  tttCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGt                       >  1:7003086/1‑93 (MQ=255)

                                                                   ttCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGtt                      <  2:4745776/93‑1 (MQ=255)

                                                                     cgctcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTc                    >  1:4710956/1‑93 (MQ=255)

                                                                     cgctcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTc                    <  1:5081332/93‑1 (MQ=255)

                                                                       ctcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCAc                  >  2:5368219/1‑93 (MQ=255)

                                                                       ctcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCAc                  >  1:5612142/1‑93 (MQ=255)

                                                                        tcgctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACt                 <  2:8195761/93‑1 (MQ=255)

                                                                          gctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTcc               >  1:3363402/1‑93 (MQ=255)

                                                                          gctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTcc               >  1:7136419/1‑93 (MQ=255)

                                                                           ctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCa              <  1:4662354/93‑1 (MQ=255)

                                                                           ctTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCa              >  2:8315971/1‑93 (MQ=255)

                                                                             tCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGa            >  2:8798673/1‑93 (MQ=255)

                                                                             tCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGa            >  2:6775720/1‑93 (MQ=255)

                                                                             tCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGa            >  2:6035011/1‑93 (MQ=255)

                                                                                ggATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGGTTCACTCCAGACCt         >  1:6433256/1‑93 (MQ=255)

                                                                                  aTTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGGTGATGGGGTGGATGCCCTTGAGTTGCTGGGCGGTTTCACTACAGACCtgt       >  1:8082965/1‑92 (MQ=255)

                                                                                   ttCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCtgat      <  1:9967985/93‑1 (MQ=255)

                                                                                     cATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCtgatga    <  1:411594/93‑1 (MQ=255)

                                                                                      aTGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTgatgat   <  2:6312491/93‑1 (MQ=255)

                                                                                       tGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGata  <  1:5652142/93‑1 (MQ=255)

AGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATA  >  NC_000913/1289453‑1289632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGTG  >  NC_000913/1289450‑1289636

GTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGC                                                                                               >  SRR4302540.6038603/1‑94 (MQ=60)

GTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGC                                                                                               >  SRR4302540.7116173/1‑94 (MQ=60)

  CAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTT                                                                                             <  SRR4302540.967510/94‑1 (MQ=60)

  CAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTT                                                                                             >  SRR4302540.7185366/1‑94 (MQ=60)

   AGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTT                                                                                            >  SRR4302540.9686165/1‑94 (MQ=60)

   AGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTT                                                                                            >  SRR4302540.4753919/1‑94 (MQ=60)

   AGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTT                                                                                            <  SRR4302540.6173148/94‑1 (MQ=60)

   AGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTT                                                                                            >  SRR4302540.7229907/1‑94 (MQ=60)

    GGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTC                                                                                           >  SRR4302540.7325494/1‑94 (MQ=60)

    GGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTC                                                                                           <  SRR4302540.4401882/94‑1 (MQ=60)

      GGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCT                                                                                         <  SRR4302540.2257140/94‑1 (MQ=60)

        AGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGTATTTATGGCTTTCATCA                                                                                       >  SRR4302540.6176939/1‑94 (MQ=60)

        AGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCA                                                                                       >  SRR4302540.4003215/1‑94 (MQ=60)

        AGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCA                                                                                       >  SRR4302540.4638965/1‑94 (MQ=60)

          AGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATT                                                                                     <  SRR4302540.4094842/94‑1 (MQ=60)

          AGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATT                                                                                     <  SRR4302540.1722328/94‑1 (MQ=60)

           GAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTG                                                                                    <  SRR4302540.140865/94‑1 (MQ=60)

            AACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGG                                                                                   <  SRR4302540.8434012/94‑1 (MQ=60)

              CATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGA                                                                                 <  SRR4302540.7479115/94‑1 (MQ=60)

               ATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAG                                                                                >  SRR4302540.3763178/1‑94 (MQ=60)

               ATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAC                                                                                >  SRR4302540.4772745/1‑94 (MQ=60)

                TGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGC                                                                               <  SRR4302540.9485602/94‑1 (MQ=60)

                TGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGC                                                                               <  SRR4302540.331527/94‑1 (MQ=60)

                 GACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCG                                                                              <  SRR4302540.5710912/94‑1 (MQ=60)

                   CGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGAC                                                                            <  SRR4302540.2206785/94‑1 (MQ=60)

                   CGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGAC                                                                            <  SRR4302540.6146227/94‑1 (MQ=60)

                    GCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACA                                                                           >  SRR4302540.1016806/1‑94 (MQ=60)

                     CAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAA                                                                          >  SRR4302540.8659346/1‑94 (MQ=60)

                     CAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAA                                                                          >  SRR4302540.2803069/1‑94 (MQ=60)

                      AGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAAC                                                                         >  SRR4302540.6091532/1‑94 (MQ=60)

                      AGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAAC                                                                         <  SRR4302540.6056279/94‑1 (MQ=60)

                      AGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAAC                                                                         >  SRR4302540.6152954/1‑94 (MQ=60)

                      AGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAAC                                                                         >  SRR4302540.1401388/1‑94 (MQ=60)

                      AGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAAC                                                                         <  SRR4302540.6056278/94‑1 (MQ=60)

                      AGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAAC                                                                         >  SRR4302540.5490904/1‑94 (MQ=60)

                      AGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAAC                                                                         >  SRR4302540.5291006/1‑94 (MQ=60)

                      AGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAAC                                                                         <  SRR4302540.261976/94‑1 (MQ=60)

                       GCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACG                                                                        >  SRR4302540.3623703/1‑94 (MQ=60)

                       GCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACG                                                                        <  SRR4302540.8469330/94‑1 (MQ=60)

                       GCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACG                                                                        <  SRR4302540.8469331/94‑1 (MQ=60)

                        CCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGG                                                                       <  SRR4302540.124598/94‑1 (MQ=60)

                        CCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGG                                                                       >  SRR4302540.1424754/1‑94 (MQ=60)

                         CATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGT                                                                      >  SRR4302540.1629490/1‑94 (MQ=60)

                         CATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGA                                                                      >  SRR4302540.1629489/1‑94 (MQ=60)

                          ATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTA                                                                     >  SRR4302540.7001822/1‑94 (MQ=60)

                          ATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTA                                                                     >  SRR4302540.7279960/1‑94 (MQ=60)

                          ATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTA                                                                     <  SRR4302540.2412303/94‑1 (MQ=60)

                            TGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACT                                                                   <  SRR4302540.4007942/94‑1 (MQ=60)

                            TGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACT                                                                   <  SRR4302540.1645521/94‑1 (MQ=60)

                            TGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACT                                                                   <  SRR4302540.5923029/94‑1 (MQ=60)

                            TGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACT                                                                   <  SRR4302540.6185475/94‑1 (MQ=60)

                            TGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACT                                                                   <  SRR4302540.2842776/94‑1 (MQ=60)

                             GGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTG                                                                  >  SRR4302540.1198774/1‑94 (MQ=60)

                               TCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGC                                                                <  SRR4302540.1227209/94‑1 (MQ=60)

                                  GAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGC                                                             >  SRR4302540.747071/1‑94 (MQ=60)

                                  GAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGC                                                             >  SRR4302540.8929044/1‑94 (MQ=60)

                                  GAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTAGTAGCGACAACGGTACTTGCGGC                                                             >  SRR4302540.8500788/1‑94 (MQ=60)

                                   AAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCT                                                            <  SRR4302540.9161159/94‑1 (MQ=60)

                                   AAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCT                                                            <  SRR4302540.5350992/94‑1 (MQ=60)

                                     AACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGA                                                          >  SRR4302540.10125793/1‑94 (MQ=60)

                                      ACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTGCTCATTGGGAGCGACAACGGTACTGGCGGCTGAT                                                         <  SRR4302540.934911/94‑1 (MQ=60)

                                      ACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGAT                                                         <  SRR4302540.2886243/94‑1 (MQ=60)

                                      ACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGAT                                                         <  SRR4302540.2490425/94‑1 (MQ=60)

                                      ACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGAT                                                         <  SRR4302540.2360579/94‑1 (MQ=60)

                                       CAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATG                                                        >  SRR4302540.699280/1‑94 (MQ=60)

                                       CAGATTCTCATTGTTGAAGATGAGCAGGGATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATG                                                        >  SRR4302540.9210863/1‑94 (MQ=60)

                                        AGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGG                                                       >  SRR4302540.6825347/1‑94 (MQ=60)

                                        AGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGG                                                       <  SRR4302540.4222074/94‑1 (MQ=60)

                                        AGATTCTCATTGTTCAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGG                                                       <  SRR4302540.7439792/94‑1 (MQ=60)

                                          ATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGG                                                     <  SRR4302540.2154777/94‑1 (MQ=60)

                                          ATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGG                                                     >  SRR4302540.6432905/1‑94 (MQ=60)

                                           TTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGCTTCATGGCTTTGCTCATTGGTAGCGACAACGGTACTGGCGGCTGATGGGGG                                                    >  SRR4302540.6968995/1‑94 (MQ=60)

                                           TTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGT                                                    <  SRR4302540.8323208/94‑1 (MQ=60)

                                           TTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGT                                                    <  SRR4302540.2545258/94‑1 (MQ=60)

                                           TTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGT                                                    <  SRR4302540.3626553/94‑1 (MQ=60)

                                           TTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGG                                                    >  SRR4302540.6968994/1‑94 (MQ=60)

                                               CATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGAT                                                <  SRR4302540.9924537/94‑1 (MQ=60)

                                               CATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGAT                                                <  SRR4302540.3005602/94‑1 (MQ=60)

                                               CATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGAT                                                <  SRR4302540.6070981/94‑1 (MQ=60)

                                               CATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGAT                                                <  SRR4302540.5745693/94‑1 (MQ=60)

                                               CATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGAT                                                <  SRR4302540.5745694/94‑1 (MQ=60)

                                                 TTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGGGGATGC                                              >  SRR4302540.8423716/1‑94 (MQ=60)

                                                   GTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCC                                            <  SRR4302540.4218739/94‑1 (MQ=60)

                                                    TTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCT                                           <  SRR4302540.7491787/94‑1 (MQ=60)

                                                     TGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTT                                          >  SRR4302540.9316531/1‑94 (MQ=60)

                                                     TGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTT                                          <  SRR4302540.6338447/94‑1 (MQ=60)

                                                      GAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTG                                         >  SRR4302540.290974/1‑94 (MQ=60)

                                                       AAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGG                                        >  SRR4302540.349768/1‑94 (MQ=60)

                                                        AGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAG                                       >  SRR4302540.2274470/1‑94 (MQ=60)

                                                         GATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGT                                      <  SRR4302540.8307461/94‑1 (MQ=60)

                                                             AGCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCT                                  <  SRR4302540.2529302/94‑1 (MQ=60)

                                                              GCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTG                                 >  SRR4302540.2235991/1‑94 (MQ=60)

                                                              GCAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTG                                 >  SRR4302540.4280338/1‑94 (MQ=60)

                                                               CAGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGG                                >  SRR4302540.5273954/1‑94 (MQ=60)

                                                                AGGTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGG                               <  SRR4302540.5958247/94‑1 (MQ=60)

                                                                  GTATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAG                             >  SRR4302540.5378247/1‑94 (MQ=60)

                                                                   TATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGG                            >  SRR4302540.9139147/1‑94 (MQ=60)

                                                                    ATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGT                           >  SRR4302540.2625379/1‑94 (MQ=60)

                                                                    ATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGT                           >  SRR4302540.7080788/1‑94 (MQ=60)

                                                                    ATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGT                           >  SRR4302540.1176357/1‑94 (MQ=60)

                                                                    ATTTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGT                           >  SRR4302540.10163428/1‑94 (MQ=60)

                                                                      TTCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTT                         <  SRR4302540.6123388/94‑1 (MQ=60)

                                                                       TCGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTC                        >  SRR4302540.4761303/1‑94 (MQ=60)

                                                                        CGCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCA                       <  SRR4302540.5136272/94‑1 (MQ=60)

                                                                         GCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCAC                      >  SRR4302540.2870246/1‑94 (MQ=60)

                                                                         GCTCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCAC                      >  SRR4302540.5673877/1‑94 (MQ=60)

                                                                           TCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTC                    <  SRR4302540.7328417/94‑1 (MQ=60)

                                                                           TCGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTC                    >  SRR4302540.1459835/1‑94 (MQ=60)

                                                                            CGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCC                   >  SRR4302540.7215928/1‑94 (MQ=60)

                                                                            CGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCC                   >  SRR4302540.2618440/1‑94 (MQ=60)

                                                                            CGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCC                   >  SRR4302540.3400211/1‑94 (MQ=60)

                                                                            CGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCC                   >  SRR4302540.505024/1‑94 (MQ=60)

                                                                            CGCTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCC                   >  SRR4302540.654410/1‑94 (MQ=60)

                                                                              CTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAG                 >  SRR4302540.2533732/1‑94 (MQ=60)

                                                                              CTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAG                 <  SRR4302540.4712261/94‑1 (MQ=60)

                                                                              CTTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATCGGGTGGATGCCCTTGAGTTGCTCGGCGGTTTTCCTCCAG                 >  SRR4302540.6725559/1‑94 (MQ=60)

                                                                               TTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGCGTTGCTGGGAGGTTTCACTCCAGA                >  SRR4302540.7625732/1‑94 (MQ=60)

                                                                               TTCTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGA                <  SRR4302540.4402684/94‑1 (MQ=60)

                                                                                 CTGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACC              >  SRR4302540.5732891/1‑94 (MQ=60)

                                                                                  TGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCT             <  SRR4302540.4670931/94‑1 (MQ=60)

                                                                                  TGGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGGTTCACTCCAGACCT             >  SRR4302540.6504864/1‑94 (MQ=60)

                                                                                   GGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTG            >  SRR4302540.2474113/1‑94 (MQ=60)

                                                                                   GGATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTG            >  SRR4302540.3323434/1‑94 (MQ=60)

                                                                                    GATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGGTGATGGGGTGGATGCCCTTGAGTTGCTGGGCGGTTTCACTACAGACCTGT           >  SRR4302540.8176777/1‑94 (MQ=60)

                                                                                    GATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGA           >  SRR4302540.8176776/1‑94 (MQ=60)

                                                                                     ATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGAT          >  SRR4302540.7143557/1‑94 (MQ=60)

                                                                                     ATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGAT          <  SRR4302540.1097432/94‑1 (MQ=60)

                                                                                     ATTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGAT          <  SRR4302540.3067357/94‑1 (MQ=60)

                                                                                      TTCATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATG         <  SRR4302540.10084273/94‑1 (MQ=60)

                                                                                        CATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGAT       <  SRR4302540.415911/94‑1 (MQ=60)

                                                                                        CATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGAT       <  SRR4302540.896356/94‑1 (MQ=60)

                                                                                         ATGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATA      >  SRR4302540.6271604/1‑94 (MQ=60)

                                                                                          TGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATAT     <  SRR4302540.5714372/94‑1 (MQ=60)

                                                                                          TGGCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATAT     <  SRR4302540.4680082/94‑1 (MQ=60)

                                                                                            GCTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGT   <  SRR4302540.7556279/94‑1 (MQ=60)

                                                                                             CTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGTG  <  SRR4302540.5541262/94‑1 (MQ=60)

GTCAGGGGAGAGAACATGACGCAGCCATTGGTCGGAAAACAGATTCTCATTGTTGAAGATGAGCAGGTATTTCGCTCGCTTCTGGATTCATGGTTTTCCTCATTGGGAGCGACAACGGTACTGGCGGCTGATGGGGTGGATGCCCTTGAGTTGCTGGGAGGTTTCACTCCAGACCTGATGATATGTG  >  NC_000913/1289450‑1289636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`