Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,418,817	(G)_6→7	coding (110/423 nt)	ydaT →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,418,811	1	.	G	99.4%	529.9 / ‑4.3	165	coding (104/423 nt)	ydaT	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (82/82); total (82/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 201 total aligned reads displayed.

GCATGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑A‑‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGA  >  NC_000913/1418719‑1418897

 cATGAGCACATCGATTCAGTGTTGTTTGCCCAAGCAGCCGAAAAAGAGCAGGCATGGGTAGCCAATGCAAAAACTGAAGAATATCTGCGCC‑A‑‑g                                                                                        <  2:6295019/93‑1 (MQ=255)

 cATGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑A‑‑g                                                                                        <  1:2774924/93‑1 (MQ=255)

  aTGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCGAATGCAATTACTGAAGAATATCTGCGCC‑A‑‑g‑g                                                                                      >  2:96277/1‑93 (MQ=255)

  aTGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑A‑‑g‑g                                                                                      <  1:5740112/93‑1 (MQ=255)

   tGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑A‑‑g‑gg                                                                                     >  1:3953174/1‑93 (MQ=255)

   tGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑A‑‑g‑gg                                                                                     <  1:4076950/93‑1 (MQ=255)

   tGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑A‑‑g‑gg                                                                                     >  2:4129367/1‑93 (MQ=255)

    gAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑A‑‑g‑ggg                                                                                    <  1:5467516/93‑1 (MQ=255)

     aGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑A‑‑g‑gggg                                                                                   <  1:3572781/93‑1 (MQ=255)

     aGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑A‑‑g‑gggg                                                                                   >  2:3949272/1‑93 (MQ=255)

      gCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑A‑‑g‑ggggg                                                                                  <  1:4782225/93‑1 (MQ=255)

        acaTCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑A‑‑g‑ggggggc                                                                                <  2:1639004/93‑3 (MQ=255)

        acaTCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑A‑‑g‑ggggggc                                                                                >  1:3011268/1‑91 (MQ=255)

        acaTCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑A‑‑g‑ggggggc                                                                                <  2:4820888/93‑3 (MQ=255)

         caTCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑A‑‑g‑ggggggcg                                                                               <  1:3028793/93‑4 (MQ=255)

          aTCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑A‑‑g‑ggggggcgg                                                                              >  1:4788589/1‑89 (MQ=255)

             gAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGa                                                                            >  2:5637933/1‑93 (MQ=255)

              aaTCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGaa                                                                           <  2:1585585/93‑1 (MQ=255)

              aaTCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGaa                                                                           >  2:1765783/1‑93 (MQ=255)

                tCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAAtt                                                                         >  2:6016246/1‑93 (MQ=255)

                tCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAAtt                                                                         <  1:1463218/93‑1 (MQ=255)

                  aGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGc                                                                       >  2:1178799/1‑93 (MQ=255)

                  aGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGc                                                                       >  2:2503744/1‑93 (MQ=255)

                  aGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGc                                                                       >  2:489404/1‑93 (MQ=255)

                     gttgttTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGcccc                                                                    <  2:3367478/93‑1 (MQ=255)

                      ttgttTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCt                                                                   <  2:2402674/93‑1 (MQ=255)

                      ttgttTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCt                                                                   >  2:1974024/1‑93 (MQ=255)

                      ttgttTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCt                                                                   <  1:95774/93‑1 (MQ=255)

                       tgttTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTg                                                                  >  1:4893756/1‑93 (MQ=255)

                             cccTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCa                                                            >  2:5784669/1‑93 (MQ=255)

                             cccTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCa                                                            <  1:875465/93‑1 (MQ=255)

                              ccTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAg                                                           >  2:4442981/1‑93 (MQ=255)

                              ccTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAg                                                           <  1:599654/93‑1 (MQ=255)

                               cTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAgg                                                          >  1:5795176/1‑93 (MQ=255)

                                tAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGc                                                         >  2:3312415/1‑93 (MQ=255)

                                tAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGc                                                         <  2:1796699/93‑1 (MQ=255)

                                  gcagcCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCag                                                       >  1:692446/1‑92 (MQ=255)

                                  gcagcCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCaa                                                       >  2:5072489/1‑93 (MQ=255)

                                  gcagcCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCaa                                                       <  2:4593081/93‑1 (MQ=255)

                                   cagcCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAg                                                      <  1:1011065/93‑1 (MQ=255)

                                   cagcCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAg                                                      <  1:4033792/93‑1 (MQ=255)

                                     gcCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGa                                                    >  1:5707323/1‑93 (MQ=255)

                                      ccGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGAc                                                   <  1:5839267/93‑1 (MQ=255)

                                      ccGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGAc                                                   <  1:3329605/93‑1 (MQ=255)

                                      ccGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGAc                                                   <  1:3144923/93‑1 (MQ=255)

                                       cGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACt                                                  >  2:5936689/1‑93 (MQ=255)

                                       cGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACt                                                  >  1:4728260/1‑93 (MQ=255)

                                       cGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACt                                                  <  2:3869776/93‑1 (MQ=255)

                                         aaaaaGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTgg                                                <  1:1005073/93‑1 (MQ=255)

                                           aaaGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGaa                                              >  2:941685/1‑93 (MQ=255)

                                           aaaGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGaa                                              <  1:6107206/93‑1 (MQ=255)

                                               ggcaggcaTGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAAt                                          <  1:3737137/93‑1 (MQ=255)

                                                gcaggcaTGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATc                                         <  2:4984001/93‑1 (MQ=255)

                                                 caggcaTGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATca                                        >  1:4543473/1‑93 (MQ=255)

                                                  aggcaTGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATcag                                       <  1:1911793/93‑1 (MQ=255)

                                                  aggcaTGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATcag                                       >  2:3529546/1‑93 (MQ=255)

                                                  aggcaTGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATcag                                       >  1:214520/1‑93 (MQ=255)

                                                   ggcaTGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATcagc                                      >  1:1399397/1‑93 (MQ=255)

                                                    gcaTGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTCGAACAATcagca                                     <  1:3403597/93‑1 (MQ=255)

                                                    ccgtGGCTAGCCAATGCAATCCGTAAAGACGACGTGCGCCGG‑‑G‑GGGGGCGGCGAATAGCCGCTGGGTCCAGGCAAGGACTGGAACAATcagca                                     <  2:5624106/90‑1 (MQ=255)

                                                     caTGGGTAGCCAATGCAATTACTGAAGAATATCTGTGCCAA‑‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACCATcagcag                                    >  2:5354985/1‑93 (MQ=255)

                                                       tGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGaa                                  <  2:4485871/93‑1 (MQ=255)

                                                        gggTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAat                                 >  2:2031536/1‑93 (MQ=255)

                                                         ggTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAata                                <  1:4281366/93‑1 (MQ=255)

                                                           tAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAAtatc                              <  1:6291348/93‑1 (MQ=255)

                                                           tAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAAtatc                              >  2:3156141/1‑93 (MQ=255)

                                                           tAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAAtatc                              <  1:5360595/93‑1 (MQ=255)

                                                           tAGCCAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGAGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAAtatc                              >  2:3624442/1‑93 (MQ=255)

                                                              ccAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAAtatctat                           <  1:1717988/93‑1 (MQ=255)

                                                               cAATGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGGGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAAtatctatc                          >  2:5506666/1‑93 (MQ=255)

                                                                aaTGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCa                         >  2:2863799/1‑93 (MQ=255)

                                                                aaTGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCa                         <  1:2806293/93‑1 (MQ=255)

                                                                 gtGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCAc                        >  2:6050379/2‑93 (MQ=255)

                                                                 aTGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCAc                        <  2:1415987/93‑1 (MQ=255)

                                                                  tGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCAcc                       >  1:4988216/1‑93 (MQ=255)

                                                                  tGCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCAcc                       >  1:1491097/1‑93 (MQ=255)

                                                                  tGCAATTACTGAAGAATATCTGCGCC‑A‑‑GTGGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCAcc                       <  2:4523327/93‑1 (MQ=255)

                                                                   gCAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCg                      <  2:612543/93‑1 (MQ=255)

                                                                    cAATTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCgt                     >  2:1354081/1‑93 (MQ=255)

                                                                     aaTTACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCgtt                    >  2:2161467/1‑93 (MQ=255)

                                                                       ttACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCgttgg                  <  2:1088481/93‑1 (MQ=255)

                                                                       ttACTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCgttgg                  <  1:1520947/93‑1 (MQ=255)

                                                                          cTGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCgttggttg               <  1:1243226/93‑1 (MQ=255)

                                                                           tGAAGAATATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGa              <  1:5753504/93‑1 (MQ=255)

                                                                            gaagaaTATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGaa             >  2:2389301/1‑93 (MQ=255)

                                                                            gaagaaTATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGaa             <  2:4640273/93‑1 (MQ=255)

                                                                            gaagaaTATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGaa             >  1:1072026/1‑93 (MQ=255)

                                                                             aagaaTATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACGATCAGCAGAATATCTATCACCGTTGGTTGaaa            >  1:3838523/1‑93 (MQ=255)

                                                                              agaaTATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAg           >  2:2237308/1‑93 (MQ=255)

                                                                               gaaTATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAgg          >  2:2910238/1‑93 (MQ=255)

                                                                                aaTATCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGt         <  2:773047/93‑1 (MQ=255)

                                                                                 atatCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTg        <  1:2463793/93‑1 (MQ=255)

                                                                                 atatCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTg        <  1:6043400/93‑1 (MQ=255)

                                                                                  tatCTGCGCC‑AGGG‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTg        >  1:3378914/1‑93 (MQ=255)

                                                                                  tatCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGa       >  2:3808591/1‑93 (MQ=255)

                                                                                   atCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGaa      <  1:4864993/93‑1 (MQ=255)

                                                                                   atCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGaa      >  1:3783696/1‑93 (MQ=255)

                                                                                    tCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGaaa     <  2:5161350/93‑1 (MQ=255)

                                                                                    tCTGCGCC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGaaa     >  2:2701327/1‑93 (MQ=255)

                                                                                       gcgcC‑AG‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGa  >  2:3678379/1‑93 (MQ=255)

GCATGAGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCC‑A‑‑G‑GGGGGCGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGA  >  NC_000913/1418719‑1418897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCA‑‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACGCAAAGAGA  >  NC_000913/1418724‑1418910

AGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCA‑‑GGGGGG                                                                                                <  SRR4302533.6076125/94‑1 (MQ=60)

AGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCA‑‑GGGGGG                                                                                                <  SRR4302533.1538883/94‑1 (MQ=60)

AGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCA‑‑GGGGGG                                                                                                <  SRR4302533.3594794/94‑1 (MQ=60)

 GCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCA‑‑GGGGGG‑G                                                                                              <  SRR4302533.4811200/94‑1 (MQ=60)

 GCACACCGAATCAGTGTTGTTCGCCTTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCA‑‑GGGGGG‑G                                                                                              <  SRR4302533.3948114/94‑1 (MQ=60)

  CACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCA‑‑GGGGGGgc                                                                                              >  SRR4302533.616144/1‑92 (MQ=60)

  CACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCA‑‑GGGGGGgc                                                                                              >  SRR4302533.3029692/1‑92 (MQ=60)

    CATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGG                                                                                            <  SRR4302533.3047327/94‑1 (MQ=60)

    CATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGG                                                                                            >  SRR4302533.4817595/1‑94 (MQ=60)

     ATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGC                                                                                           >  SRR4302533.1117726/1‑94 (MQ=60)

     ATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGC                                                                                           >  SRR4302533.5768390/1‑94 (MQ=60)

     ATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCA‑‑GGGGGGgcggg                                                                                           >  SRR4302533.3415465/1‑89 (MQ=60)

           TCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTG                                                                                     <  SRR4302533.1472038/94‑1 (MQ=60)

             AGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCC                                                                                   >  SRR4302533.1448358/1‑94 (MQ=60)

              GTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCC                                                                                  >  SRR4302533.5438013/1‑94 (MQ=60)

               TGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCC                                                                                 >  SRR4302533.476063/1‑94 (MQ=60)

                GTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCT                                                                                <  SRR4302533.3795579/94‑1 (MQ=60)

                GTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCT                                                                                <  SRR4302533.4062687/94‑1 (MQ=60)

                 TTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTG                                                                               <  SRR4302533.96375/94‑1 (MQ=60)

                 TTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTG                                                                               >  SRR4302533.4923416/1‑94 (MQ=60)

                  TGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGG                                                                              >  SRR4302533.3027752/1‑94 (MQ=60)

                  TGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGG                                                                              <  SRR4302533.722387/94‑1 (MQ=60)

                    TTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTT                                                                            <  SRR4302533.405612/94‑1 (MQ=60)

                       TCCCTAGCAGCAGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTCCTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCA                                                                         <  SRR4302533.1230796/94‑1 (MQ=60)

                       GCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCA                                                                         <  SRR4302533.5823767/94‑1 (MQ=60)

                       GCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCA                                                                         >  SRR4302533.4825871/1‑94 (MQ=60)

                        CCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAG                                                                        >  SRR4302533.5781270/1‑94 (MQ=60)

                        CCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAG                                                                        <  SRR4302533.3045739/94‑1 (MQ=60)

                        CCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAG                                                                        <  SRR4302533.880606/94‑1 (MQ=60)

                         CCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGG                                                                       <  SRR4302533.603182/94‑1 (MQ=60)

                         CCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGG                                                                       >  SRR4302533.5830188/1‑94 (MQ=60)

                            AGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAG                                                                    >  SRR4302533.696535/1‑94 (MQ=60)

                              CAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGG                                                                  <  SRR4302533.3497261/94‑1 (MQ=60)

                              CAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGG                                                                  <  SRR4302533.1016981/94‑1 (MQ=60)

                              CAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGG                                                                  <  SRR4302533.4058344/94‑1 (MQ=60)

                               AGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGGATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGA                                                                 >  SRR4302533.1479031/1‑94 (MQ=60)

                               AGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGA                                                                 >  SRR4302533.5741797/1‑94 (MQ=60)

                                 CCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACT                                                               <  SRR4302533.5874571/94‑1 (MQ=60)

                                 CCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACT                                                               >  SRR4302533.4756924/1‑94 (MQ=60)

                                 CCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACT                                                               >  SRR4302533.3850463/1‑94 (MQ=60)

                                 CCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACT                                                               <  SRR4302533.3164259/94‑1 (MQ=60)

                                 CCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACT                                                               <  SRR4302533.3350185/94‑1 (MQ=60)

                                  CGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTG                                                              <  SRR4302533.1014072/94‑1 (MQ=60)

                                   GAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGG                                                             <  SRR4302533.313010/94‑1 (MQ=60)

                                   GAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGG                                                             <  SRR4302533.4996264/94‑1 (MQ=60)

                                    AAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGA                                                            <  SRR4302533.1010951/94‑1 (MQ=60)

                                      AAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAAC                                                          <  SRR4302533.6144080/94‑1 (MQ=60)

                                      AAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAAC                                                          >  SRR4302533.638853/1‑94 (MQ=60)

                                         GGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAAT                                                       >  SRR4302533.3695646/1‑94 (MQ=60)

                                          GGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATC                                                      <  SRR4302533.3760061/94‑1 (MQ=60)

                                           GCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCA                                                     >  SRR4302533.5849858/1‑94 (MQ=60)

                                           GCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCA                                                     >  SRR4302533.4570987/1‑94 (MQ=60)

                                            CAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAG                                                    >  SRR4302533.215869/1‑94 (MQ=60)

                                             AGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGC                                                   >  SRR4302533.886635/1‑94 (MQ=60)

                                             AGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGC                                                   >  SRR4302533.1407832/1‑94 (MQ=60)

                                             AGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGC                                                   <  SRR4302533.1923456/94‑1 (MQ=60)

                                               GCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAG                                                 <  SRR4302533.280941/94‑1 (MQ=60)

                                               GCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTCGAACAATCAGCAG                                                 <  SRR4302533.3424614/94‑1 (MQ=60)

                                                 ATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAA                                               >  SRR4302533.5546125/1‑94 (MQ=60)

                                                  TGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAAT                                              <  SRR4302533.2599296/94‑1 (MQ=60)

                                                    GGTAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATAT                                            <  SRR4302533.4307102/94‑1 (MQ=60)

                                                      TAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCT                                          <  SRR4302533.6328863/94‑1 (MQ=60)

                                                      TAGCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCT                                          <  SRR4302533.5392790/94‑1 (MQ=60)

                                                        GCCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTAT                                        <  SRR4302533.6118663/94‑1 (MQ=60)

                                                         CCAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATC                                       <  SRR4302533.1728403/94‑1 (MQ=60)

                                                          CAATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCA                                      <  SRR4302533.1510408/94‑1 (MQ=60)

                                                           AATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCAC                                     <  SRR4302533.2823564/94‑1 (MQ=60)

                                                            ATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACC                                    <  SRR4302533.4566162/94‑1 (MQ=60)

                                                            ATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACC                                    >  SRR4302533.5018461/1‑94 (MQ=60)

                                                            ATGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACC                                    >  SRR4302533.1500089/1‑94 (MQ=60)

                                                             TGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCCCCG                                   >  SRR4302533.6025778/1‑94 (MQ=60)

                                                             TGCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCG                                   >  SRR4302533.104557/1‑94 (MQ=60)

                                                              TCAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGT                                  <  SRR4302533.427205/94‑1 (MQ=60)

                                                               CAATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTT                                 >  SRR4302533.1837021/1‑94 (MQ=60)

                                                                AATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTG                                <  SRR4302533.5128865/94‑1 (MQ=60)

                                                                 ATTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGG                               >  SRR4302533.3269071/1‑94 (MQ=60)

                                                                  TTACTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGT                              <  SRR4302533.1530104/94‑1 (MQ=60)

                                                                     CTGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGA                           <  SRR4302533.1250649/94‑1 (MQ=60)

                                                                      TGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAA                          >  SRR4302533.1078341/1‑94 (MQ=60)

                                                                      TGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAA                          >  SRR4302533.4727937/1‑94 (MQ=60)

                                                                      TGAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAA                          <  SRR4302533.5788215/94‑1 (MQ=60)

                                                                       GAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACGATCAGCAGAATATCTATCACCGTTGGTTGAAA                         >  SRR4302533.3861974/1‑94 (MQ=60)

                                                                       GAAGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAA                         <  SRR4302533.575797/94‑1 (MQ=60)

                                                                        AAGAACATATGCGCCA‑‑GGGGGGTTGGCAAAGTGCCCCTGGTTCCAGCCAGGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAG                        <  SRR4302533.1149707/94‑1 (MQ=60)

                                                                         AGAATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGG                       <  SRR4302533.2338974/94‑1 (MQ=60)

                                                                           AATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTG                     >  SRR4302533.3919519/1‑94 (MQ=60)

                                                                            ATATCTGCGCCAGGGGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTG                     >  SRR4302533.3399773/1‑94 (MQ=60)

                                                                            ATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGA                    <  SRR4302533.6080013/94‑1 (MQ=60)

                                                                            ATATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGA                    <  SRR4302533.2478992/94‑1 (MQ=60)

                                                                             TATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAA                   >  SRR4302533.1319695/1‑94 (MQ=60)

                                                                             TATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAA                   <  SRR4302533.2110257/94‑1 (MQ=60)

                                                                             TATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAA                   >  SRR4302533.3806865/1‑94 (MQ=60)

                                                                              ATCTGCGCCAG‑GGGGGG‑CGGCTAATTGCCCGTGGTTCCCGGCAAGGACTGTAGGAATCAGCAGAATATCTAACCCCGTTGGTTGAAAGGTGAAA                  <  SRR4302533.4894477/94‑1 (MQ=46)

                                                                              ATCTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAA                  <  SRR4302533.4894476/94‑1 (MQ=60)

                                                                                CTGCGCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACG                >  SRR4302533.3413835/1‑94 (MQ=60)

                                                                                    GCCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAA            >  SRR4302533.2124804/1‑94 (MQ=60)

                                                                                     CCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAAC           <  SRR4302533.5745575/94‑1 (MQ=60)

                                                                                     CCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAAC           <  SRR4302533.3513804/94‑1 (MQ=60)

                                                                                     CCAG‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAAC           <  SRR4302533.4306498/94‑1 (MQ=60)

                                                                                       cagGGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACG          <  SRR4302533.6222983/91‑1 (MQ=60)

                                                                                       cagGGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACG          >  SRR4302533.2098838/4‑94 (MQ=60)

                                                                                       cagGGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACG          <  SRR4302533.5040449/91‑1 (MQ=60)

                                                                                       cagGGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACG          >  SRR4302533.1086051/4‑94 (MQ=60)

                                                                                        agGGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACGC         >  SRR4302533.6172867/3‑94 (MQ=60)

                                                                                        agGGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACGC         >  SRR4302533.1654507/3‑94 (MQ=60)

                                                                                          GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACGCAA       <  SRR4302533.1882869/94‑1 (MQ=60)

                                                                                            GGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACGCAAAG     <  SRR4302533.3831870/94‑1 (MQ=60)

                                                                                              GG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACGCAAAGAG   <  SRR4302533.413397/94‑1 (MQ=60)

                                                                                               G‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACGCAAAGAGA  <  SRR4302533.968810/94‑1 (MQ=60)

AGCACATCGAATCAGTGTTGTTTGCCCTAGCAGCCGAAAAAGGGCAGGCATGGGTAGCCAATGCAATTACTGAAGAATATCTGCGCCA‑‑GGGGGG‑CGGCGAATTGCCCCTGGTTCCAGGCAAGGACTGGAACAATCAGCAGAATATCTATCACCGTTGGTTGAAAGGTGAAACGAAAACGCAAAGAGA  >  NC_000913/1418724‑1418910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`