Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,472,237 A→G intergenic (+3704/‑8) tra82 → / → ydbC IS30 transposase/putative dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,472,2370AG99.2% 815.8 / ‑5.0 256intergenic (+3704/‑8)tra82/ydbCIS30 transposase/putative dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (138/116);  total (138/118)
Fisher's exact test for biased strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

AACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAG  >  NC_000913/1472147‑1472326
                                                                                          |                                                                                         
  cTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGt                                                                                       <  1:2303084/93‑1 (MQ=255)
   tCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTg                                                                                      <  2:2841724/93‑1 (MQ=255)
      tCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGa                                                                                   <  2:201803/93‑1 (MQ=255)
      tCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGa                                                                                   >  1:3183388/1‑93 (MQ=255)
       ccAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGAt                                                                                  <  2:2527751/93‑1 (MQ=255)
         aGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGa                                                                                >  1:1153224/1‑93 (MQ=255)
          gTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGag                                                                               >  1:611149/1‑93 (MQ=255)
          gTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGag                                                                               <  1:4149247/93‑1 (MQ=255)
           tAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGagc                                                                              >  1:3061944/1‑93 (MQ=255)
            aaTCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGagca                                                                             <  2:1258311/93‑1 (MQ=255)
                 tGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAAt                                                                        <  1:393933/93‑1 (MQ=255)
                  gTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATa                                                                       >  1:3548024/1‑93 (MQ=255)
                   tttACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATAc                                                                      >  2:1612414/1‑93 (MQ=255)
                   tttACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATAc                                                                      <  2:2233554/93‑1 (MQ=255)
                    ttACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACa                                                                     <  2:3755478/93‑1 (MQ=255)
                    ttACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACa                                                                     >  1:5941406/1‑93 (MQ=255)
                    ttACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACa                                                                     <  1:1418754/93‑1 (MQ=255)
                    ttACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACa                                                                     >  2:5143712/1‑93 (MQ=255)
                    ttACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACa                                                                     <  2:3167639/93‑1 (MQ=255)
                     tACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACAt                                                                    >  1:4564896/1‑93 (MQ=255)
                      tcacCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACAtt                                                                   <  1:6227319/92‑1 (MQ=255)
                       cacCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACAttt                                                                  >  1:3714596/1‑93 (MQ=255)
                         ccTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTAc                                                                <  1:878919/93‑1 (MQ=255)
                          cTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTAct                                                               <  2:5396836/93‑1 (MQ=255)
                           ttCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTActc                                                              >  1:5247103/1‑93 (MQ=255)
                              gCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCg                                                           >  1:2796541/1‑93 (MQ=255)
                                 ttatttatCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTa                                                        <  1:2765752/93‑1 (MQ=255)
                                 ttatttatCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTa                                                        >  1:1741974/1‑93 (MQ=255)
                                 ttatttatCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTa                                                        >  1:4500321/1‑93 (MQ=255)
                                  tatttatCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTAc                                                       <  2:2731643/93‑1 (MQ=255)
                                  tatttatCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTAc                                                       >  1:5313065/1‑93 (MQ=255)
                                  tatttatCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTAc                                                       >  1:1816765/1‑93 (MQ=255)
                                  tatttatCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTAc                                                       <  2:1167247/93‑1 (MQ=255)
                                    tttatCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACaa                                                     <  2:653780/93‑1 (MQ=255)
                                     ttatCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACaaa                                                    >  1:2343349/1‑93 (MQ=255)
                                      tatCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACaaaa                                                   <  1:4212545/93‑1 (MQ=255)
                                      tatCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACaaaa                                                   >  1:459078/1‑93 (MQ=255)
                                      tatCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACaaaa                                                   <  2:2469247/93‑1 (MQ=255)
                                       atCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAAt                                                  >  2:1392749/1‑93 (MQ=255)
                                        tctcTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATc                                                 <  2:2148267/93‑1 (MQ=255)
                                        tctcTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATc                                                 >  1:2844619/1‑93 (MQ=255)
                                        tctcTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATc                                                 >  2:3589918/1‑93 (MQ=255)
                                         ctctTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATcc                                                <  1:5259352/93‑1 (MQ=255)
                                           cttttcttttCAATACTGATTTTTCTTAA‑CCGGTTTATTAACAGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGtt                                             <  1:3650911/93‑1 (MQ=255)
                                            ttttCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGtt                                             >  1:3286569/1‑93 (MQ=255)
                                            ttttCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGtt                                             <  2:3032782/93‑1 (MQ=255)
                                              ttCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTaa                                           >  2:941992/1‑93 (MQ=255)
                                                 gTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCg                                        <  1:2653251/93‑1 (MQ=255)
                                                  tttCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGt                                       >  1:192061/1‑93 (MQ=255)
                                                  tttCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGt                                       >  2:4826666/1‑93 (MQ=255)
                                                   ttCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTc                                      >  1:3354004/1‑93 (MQ=255)
                                                     cTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCtt                                    >  1:4479482/1‑93 (MQ=255)
                                                     cTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCtt                                    <  1:1112185/93‑1 (MQ=255)
                                                     cTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCtt                                    <  2:5720666/93‑1 (MQ=255)
                                                      tataCTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTg                                   <  2:5012019/93‑1 (MQ=255)
                                                      tataCTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTg                                   >  2:3527378/1‑93 (MQ=255)
                                                      tataCTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGATGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTg                                   <  2:3078819/93‑1 (MQ=255)
                                                       ataCTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTgg                                  <  2:185580/93‑1 (MQ=255)
                                                       ataCTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTgg                                  >  2:426206/1‑93 (MQ=255)
                                                       ataCTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTgg                                  >  1:5624286/1‑93 (MQ=255)
                                                       ataCTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTgg                                  <  1:767492/93‑1 (MQ=255)
                                                        taCTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGt                                 >  2:6254692/1‑93 (MQ=255)
                                                        taCTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGt                                 >  1:1992940/1‑93 (MQ=255)
                                                        taCTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGt                                 >  1:1367965/1‑93 (MQ=255)
                                                        taCTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGt                                 >  2:6009235/1‑93 (MQ=255)
                                                        taCTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGt                                 <  2:334338/93‑1 (MQ=255)
                                                          cTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTa                               >  2:4653443/1‑93 (MQ=255)
                                                          cTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTa                               >  2:4340344/1‑93 (MQ=255)
                                                          cTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTa                               <  2:489687/93‑1 (MQ=255)
                                                          cTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTa                               <  1:3816187/93‑1 (MQ=255)
                                                           tGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTAt                              <  2:5946722/93‑1 (MQ=255)
                                                            gATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATg                             <  2:5202135/93‑1 (MQ=255)
                                                            gATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATg                             >  2:3428110/1‑93 (MQ=255)
                                                             aTTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATgg                            >  1:5511574/1‑93 (MQ=255)
                                                              tttttCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGgc                           <  2:4732534/93‑1 (MQ=255)
                                                               ttttCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGgcg                          >  2:4411289/1‑93 (MQ=255)
                                                                 ttCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGcgcg                        >  1:167367/1‑93 (MQ=255)
                                                                  tCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGa                       >  2:3277754/1‑93 (MQ=255)
                                                                   cTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGAt                      <  2:4010607/93‑1 (MQ=255)
                                                                      aaTCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCa                   >  2:4016811/1‑93 (MQ=255)
                                                                       aTCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCaa                  >  1:4429852/1‑93 (MQ=255)
                                                                        tCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAAc                 <  2:846636/93‑1 (MQ=255)
                                                                        tCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAAc                 <  2:2430904/93‑1 (MQ=255)
                                                                        tCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAAc                 <  1:2188540/93‑1 (MQ=255)
                                                                          cGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTg               <  1:701928/93‑1 (MQ=255)
                                                                            ttttATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGc             <  2:4111722/93‑1 (MQ=255)
                                                                            ttttATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGc             <  2:1699148/93‑1 (MQ=255)
                                                                            ttttATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGc             <  2:3925295/93‑1 (MQ=255)
                                                                             tttATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCa            <  1:1884016/93‑1 (MQ=255)
                                                                             tttATTACAGGGCGGGGAGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCa            >  2:5479995/1‑93 (MQ=255)
                                                                              ttattaCAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAg           >  2:2970447/1‑93 (MQ=255)
                                                                               tattaCAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAgg          <  1:154788/93‑1 (MQ=255)
                                                                               tattaCAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAgg          >  1:2009148/1‑93 (MQ=255)
                                                                                attaCAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGt         >  2:4530448/1‑93 (MQ=255)
                                                                                 ttaCAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTc        >  2:396222/1‑93 (MQ=255)
                                                                                  taCAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTcc       >  1:4751442/1‑93 (MQ=255)
                                                                                  taCAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTcc       <  2:4062925/93‑1 (MQ=255)
                                                                                     agggcggggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTgg    <  2:6043499/93‑1 (MQ=255)
                                                                                      gggcggggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGa   >  2:3632298/1‑93 (MQ=255)
                                                                                       ggcggggTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAg  <  1:441204/93‑1 (MQ=255)
                                                                                          |                                                                                         
AACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAG  >  NC_000913/1472147‑1472326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCAACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTT  >  NC_000913/1472145‑1472330
                                                                                            |                                                                                             
ggaactcattcaataaactttgTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGG                                                                                              <  SRR4302533.5127835/72‑1 (MQ=60)
 CAACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGG                                                                                             <  SRR4302533.6158723/94‑1 (MQ=60)
 CAACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGG                                                                                             <  SRR4302533.5963529/94‑1 (MQ=60)
 CAACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGG                                                                                             <  SRR4302533.5963347/94‑1 (MQ=60)
 CAACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGG                                                                                             >  SRR4302533.83293/1‑94 (MQ=60)
   ACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGT                                                                                           <  SRR4302533.3765445/94‑1 (MQ=60)
    CTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTG                                                                                          <  SRR4302533.2317352/94‑1 (MQ=60)
     TCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGC                                                                                         >  SRR4302533.620553/1‑94 (MQ=60)
      CATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCG                                                                                        >  SRR4302533.1856159/1‑94 (MQ=60)
      CATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCG                                                                                        <  SRR4302533.5504799/94‑1 (MQ=60)
      CATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCG                                                                                        <  SRR4302533.19907/94‑1 (MQ=60)
       ATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGA                                                                                       >  SRR4302533.3202970/1‑94 (MQ=60)
        TCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGAT                                                                                      >  SRR4302533.1308501/1‑94 (MQ=60)
        TCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGAT                                                                                      >  SRR4302533.1875155/1‑94 (MQ=60)
         CCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATG                                                                                     <  SRR4302533.3354143/94‑1 (MQ=60)
          CAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGA                                                                                    >  SRR4302533.5404325/1‑94 (MQ=60)
          CAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGA                                                                                    >  SRR4302533.1160131/1‑94 (MQ=60)
          CAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGA                                                                                    <  SRR4302533.3569238/94‑1 (MQ=60)
           AGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAG                                                                                   >  SRR4302533.614743/1‑94 (MQ=60)
            GTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGC                                                                                  <  SRR4302533.4174386/94‑1 (MQ=60)
            GTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGC                                                                                  >  SRR4302533.3080738/1‑94 (MQ=60)
                TCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCA                                                                              <  SRR4302533.2012252/94‑1 (MQ=60)
                  TTGTTTCCACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAAT                                                                            <  SRR4302533.3155354/94‑1 (MQ=60)
                  TTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATAGTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAAT                                                                            >  SRR4302533.3839872/1‑94 (MQ=60)
                  TTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAAT                                                                            <  SRR4302533.1720614/94‑1 (MQ=60)
                  TTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAAT                                                                            >  SRR4302533.3949264/1‑94 (MQ=60)
                   TGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATA                                                                           >  SRR4302533.3569889/1‑94 (MQ=60)
                   TGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATA                                                                           <  SRR4302533.396323/94‑1 (MQ=60)
                     TTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACA                                                                         >  SRR4302533.5977335/1‑94 (MQ=60)
                      TTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACAT                                                                        <  SRR4302533.1427336/94‑1 (MQ=60)
                      TTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACAT                                                                        >  SRR4302533.4592572/1‑94 (MQ=60)
                      TTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACAT                                                                        <  SRR4302533.1433975/94‑1 (MQ=60)
                       TACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATT                                                                       <  SRR4302533.3567678/94‑1 (MQ=60)
                        TCACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTT                                                                      <  SRR4302533.6264627/94‑1 (MQ=60)
                        ACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTT                                                                      >  SRR4302533.3737402/1‑94 (MQ=60)
                        ACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTT                                                                      >  SRR4302533.47039/1‑94 (MQ=60)
                           CCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACT                                                                   <  SRR4302533.884078/94‑1 (MQ=60)
                           CCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACT                                                                   >  SRR4302533.5613340/1‑94 (MQ=60)
                            CTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTC                                                                  >  SRR4302533.5278651/1‑94 (MQ=60)
                             TTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCT                                                                 <  SRR4302533.2858342/94‑1 (MQ=60)
                             TTCGCATTATTTATCTCTTCTCCTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCT                                                                 <  SRR4302533.4208487/94‑1 (MQ=60)
                              TCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTC                                                                <  SRR4302533.3749267/94‑1 (MQ=60)
                              TCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTC                                                                >  SRR4302533.747153/1‑94 (MQ=60)
                               CGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCG                                                               >  SRR4302533.2813739/1‑94 (MQ=60)
                                 CATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGT                                                             <  SRR4302533.3640228/94‑1 (MQ=60)
                                  ATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTA                                                            >  SRR4302533.4606245/1‑94 (MQ=60)
                                  ATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTA                                                            >  SRR4302533.4527578/1‑94 (MQ=60)
                                  ATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTA                                                            >  SRR4302533.1752565/1‑94 (MQ=60)
                                   TTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTAC                                                           <  SRR4302533.2782709/94‑1 (MQ=60)
                                   TTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTAC                                                           >  SRR4302533.5344904/1‑94 (MQ=60)
                                   TTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTAC                                                           >  SRR4302533.1827855/1‑94 (MQ=60)
                                    TATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACA                                                          <  SRR4302533.1718119/94‑1 (MQ=60)
                                    TATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACA                                                          <  SRR4302533.1214619/94‑1 (MQ=60)
                                    TATTTATCTCTTTTCGTATCTATACTGATTGTTCTTAATCCGTTTTATTACAGGGCGGTGTGCGATGAGCAGCAATACATTTTCTCTCGGTACA                                                          <  SRR4302533.4535322/94‑1 (MQ=60)
                                     CTTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAA                                                         >  SRR4302533.603668/1‑94 (MQ=60)
                                      TTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAA                                                        >  SRR4302533.2357854/1‑94 (MQ=60)
                                       TTATCTCTTTTCGTTTCTATACTGATTTTTCTTATTCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAA                                                       >  SRR4302533.1158411/1‑94 (MQ=60)
                                       TTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGTCGGGGAGCGATGAGCAGCAATACATTTACTCTCGGTAgaaat                                                       >  SRR4302533.858515/1‑89 (MQ=60)
                                       TTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAA                                                       >  SRR4302533.461820/1‑94 (MQ=60)
                                        TATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAAT                                                      <  SRR4302533.191302/94‑1 (MQ=60)
                                        TATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAAT                                                      <  SRR4302533.4238027/94‑1 (MQ=60)
                                         ATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATC                                                     >  SRR4302533.2862123/1‑94 (MQ=60)
                                          TCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCC                                                    <  SRR4302533.594484/94‑1 (MQ=60)
                                          TCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCC                                                    >  SRR4302533.2919114/1‑94 (MQ=60)
                                           CTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCG                                                   <  SRR4302533.6245485/94‑1 (MQ=60)
                                           CTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCG                                                   <  SRR4302533.5290930/94‑1 (MQ=60)
                                           CTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCG                                                   <  SRR4302533.4743250/94‑1 (MQ=60)
                                           CTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCG                                                   <  SRR4302533.2674713/94‑1 (MQ=60)
                                             CTTTTCTTTTCAATACTGATTTTTCTTAA‑CCGGTTTATTAACAGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTA                                                <  SRR4302533.3673359/94‑1 (MQ=59)
                                             CTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTT                                                 >  SRR4302533.3306858/1‑94 (MQ=60)
                                             CTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTT                                                 >  SRR4302533.422237/1‑94 (MQ=60)
                                              TTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTA                                                >  SRR4302533.5584146/1‑94 (MQ=60)
                                               TTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAA                                               <  SRR4302533.3703388/94‑1 (MQ=60)
                                                TTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAAC                                              <  SRR4302533.2331425/94‑1 (MQ=60)
                                                 TCGTTTCTATACTTATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACC                                             <  SRR4302533.2789799/94‑1 (MQ=60)
                                                 TCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACC                                             <  SRR4302533.2789798/94‑1 (MQ=60)
                                                   GTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGT                                           >  SRR4302533.1409187/1‑94 (MQ=60)
                                                   GTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGT                                           >  SRR4302533.193271/1‑94 (MQ=60)
                                                   GTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGT                                           <  SRR4302533.2669550/94‑1 (MQ=60)
                                                    TTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTC                                          >  SRR4302533.3374730/1‑94 (MQ=60)
                                                     TTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCT                                         >  SRR4302533.5194328/1‑94 (MQ=60)
                                                      TCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTT                                        <  SRR4302533.741271/94‑1 (MQ=60)
                                                      TCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTT                                        >  SRR4302533.1645146/1‑94 (MQ=60)
                                                      TCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTT                                        >  SRR4302533.4506580/1‑94 (MQ=60)
                                                       CTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTG                                       <  SRR4302533.1118784/94‑1 (MQ=60)
                                                       CTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTG                                       >  SRR4302533.1594565/1‑94 (MQ=60)
                                                       CTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTG                                       >  SRR4302533.5152048/1‑94 (MQ=60)
                                                        TATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGG                                      >  SRR4302533.5658281/1‑94 (MQ=60)
                                                         ATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGT                                     >  SRR4302533.2005162/1‑94 (MQ=60)
                                                         ATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGT                                     <  SRR4302533.772019/94‑1 (MQ=60)
                                                         ATACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGT                                     >  SRR4302533.1376191/1‑94 (MQ=60)
                                                          TACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTT                                    >  SRR4302533.4296094/1‑94 (MQ=60)
                                                           ACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTA                                   >  SRR4302533.4527133/1‑94 (MQ=60)
                                                           ACTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTA                                   >  SRR4302533.4519698/1‑94 (MQ=60)
                                                            CTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTAT                                  <  SRR4302533.6015139/94‑1 (MQ=60)
                                                            CTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTAT                                  >  SRR4302533.4446845/1‑94 (MQ=60)
                                                            CTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTAT                                  >  SRR4302533.1377212/1‑94 (MQ=60)
                                                            CTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTAT                                  >  SRR4302533.406127/1‑94 (MQ=60)
                                                            CTGATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTAT                                  <  SRR4302533.3839522/94‑1 (MQ=60)
                                                              GATTTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGG                                >  SRR4302533.5544849/1‑94 (MQ=60)
                                                                 TTTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGC                             >  SRR4302533.264210/1‑94 (MQ=60)
                                                                  TTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCG                            >  SRR4302533.168428/1‑94 (MQ=60)
                                                                  TTTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCG                            >  SRR4302533.5314677/1‑94 (MQ=60)
                                                                   TTCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGA                           >  SRR4302533.218492/1‑94 (MQ=60)
                                                                    TCTTAATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGAT                          <  SRR4302533.4499169/94‑1 (MQ=60)
                                                                        AATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAA                      >  SRR4302533.6303091/1‑94 (MQ=60)
                                                                        AATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAA                      >  SRR4302533.2128704/1‑94 (MQ=60)
                                                                        AATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAA                      >  SRR4302533.4456631/1‑94 (MQ=60)
                                                                         ATCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAAC                     <  SRR4302533.2723264/94‑1 (MQ=60)
                                                                          TCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACT                    >  SRR4302533.5289500/1‑94 (MQ=60)
                                                                          TCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACT                    <  SRR4302533.3217175/94‑1 (MQ=60)
                                                                          TCCGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACT                    <  SRR4302533.2202022/94‑1 (MQ=60)
                                                                            CGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGG                  <  SRR4302533.2641854/94‑1 (MQ=60)
                                                                            CGTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGG                  <  SRR4302533.706082/94‑1 (MQ=60)
                                                                             GTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCGACTGGC                 >  SRR4302533.2962562/1‑94 (MQ=60)
                                                                             GTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGC                 <  SRR4302533.1949485/94‑1 (MQ=60)
                                                                             GTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGC                 >  SRR4302533.226474/1‑94 (MQ=60)
                                                                             GTTTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACAGTCTTGGTTATGGCGCGATGCAACTGGC                 >  SRR4302533.2272782/1‑94 (MQ=60)
                                                                               TTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAG               <  SRR4302533.1895522/94‑1 (MQ=60)
                                                                               TTTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAG               <  SRR4302533.4589418/94‑1 (MQ=60)
                                                                                TTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGG              >  SRR4302533.5308789/1‑94 (MQ=60)
                                                                                TTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGG              >  SRR4302533.4259178/1‑94 (MQ=60)
                                                                                TTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGG              <  SRR4302533.4440531/94‑1 (MQ=60)
                                                                                TTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGG              <  SRR4302533.3101056/94‑1 (MQ=60)
                                                                                TTATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGG              >  SRR4302533.2021523/1‑94 (MQ=60)
                                                                                 TATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGT             <  SRR4302533.155774/94‑1 (MQ=60)
                                                                                 TATTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGT             >  SRR4302533.1922565/1‑94 (MQ=60)
                                                                                   TTACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCC           >  SRR4302533.4780249/1‑94 (MQ=60)
                                                                                    TACAGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCT          <  SRR4302533.3672399/94‑1 (MQ=60)
                                                                                     ACAGGGCGGGGGGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTG         >  SRR4302533.3324110/1‑94 (MQ=60)
                                                                                       AGGGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA       >  SRR4302533.1790588/1‑94 (MQ=60)
                                                                                         GGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGT     >  SRR4302533.3611380/1‑94 (MQ=60)
                                                                                         GGCGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGT     <  SRR4302533.443838/94‑1 (MQ=60)
                                                                                           CGGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTT   <  SRR4302533.4151111/94‑1 (MQ=60)
                                                                                            GGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTT  >  SRR4302533.6174886/1‑94 (MQ=60)
                                                                                            |                                                                                             
GCAACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATACTGATTTTTCTTAATCCGTTTTATTACAGGGCAGGGTGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTT  >  NC_000913/1472145‑1472330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑