Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,472,339	(C)_6→7	coding (95/861 nt)	ydbC →	putative dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,472,333	1	.	C	96.2%	557.3 / 13.1	184	coding (89/861 nt)	ydbC	putative dehydrogenase
Reads supporting (aligned to +/- strand): ref base . (5/2); new base C (64/111); total (71/113)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

TGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑CC‑CCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTA  >  NC_000913/1472241‑1472420

tGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTg‑‑g                                                                                            >  1:9580370/1‑93 (MQ=255)

tGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTg‑‑g                                                                                            <  2:4120141/93‑1 (MQ=255)

 gCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑c                                                                                         >  2:1540238/1‑93 (MQ=255)

 gCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑c                                                                                         <  2:1913066/93‑1 (MQ=255)

   gATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑tc‑c                                                                                      >  2:9725124/1‑93 (MQ=255)

   gATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑c                                                                                      <  2:9215396/93‑1 (MQ=255)

   gATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑c                                                                                      >  2:2862424/1‑93 (MQ=255)

   gATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑GTTTTG‑‑G‑‑cc‑c                                                                                      >  1:3745033/1‑93 (MQ=255)

   gATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTGGGTTATGGCGCGATGCAACTGGCAGGTGCTGGA‑G‑TTTTTG‑‑G‑‑ac‑c                                                                                      >  1:5645390/1‑93 (MQ=255)

     tGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑ccc                                                                                    >  1:1392684/1‑93 (MQ=255)

      gAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑cccc                                                                                   <  2:2487583/93‑1 (MQ=255)

       agcagcAATACCTTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑ccccc                                                                                  <  2:3287374/93‑2 (MQ=255)

        gcagcaATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑cccccc                                                                                 >  2:5423662/1‑91 (MQ=255)

        gcagcaATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑ccccca                                                                                 <  1:4493252/93‑3 (MQ=255)

        gcagcaATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑ccccca                                                                                 <  1:5633551/93‑3 (MQ=255)

        gcagcaATACATTTACTCTCGGTACAAAATCCGGTACCCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑ccccca                                                                                 <  1:1498954/93‑3 (MQ=255)

         cagcaATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑cccccac                                                                                <  2:1432328/93‑4 (MQ=255)

         cagcaATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑cccccac                                                                                <  2:3676748/93‑4 (MQ=255)

          agcaATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑cc‑cccccacg                                                                               >  2:806163/1‑89 (MQ=255)

             aaTACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACgaga                                                                             >  2:615387/1‑93 (MQ=255)

             aaTACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACgaga                                                                             >  2:9589067/1‑93 (MQ=255)

             aaTACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACgaga                                                                             <  1:3539901/93‑1 (MQ=255)

              aTACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGAt                                                                            >  2:6043147/1‑93 (MQ=255)

               tACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATc                                                                           <  1:458909/93‑1 (MQ=255)

                aCATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCg                                                                          >  1:3841608/1‑93 (MQ=255)

                 cATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGc                                                                         <  1:1522109/93‑1 (MQ=255)

                  aTTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGcc                                                                        >  2:389544/1‑93 (MQ=255)

                    ttACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGccgc                                                                      <  1:467598/93‑3 (MQ=255)

                     tACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCcgcg                                                                     >  1:3389584/1‑93 (MQ=255)

                      aCTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGt                                                                    <  1:8601745/93‑1 (MQ=255)

                         ctcGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGc                                                                 >  1:7724709/1‑93 (MQ=255)

                          tcGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCt                                                                >  1:3056020/1‑93 (MQ=255)

                             gTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGCCGCtata                                                             >  1:7147148/1‑93 (MQ=255)

                               aCAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GA‑CC‑CCCCACGAGATCGCCGCGTCGCTATAAc                                                           >  1:9158212/1‑93 (MQ=255)

                                 aaaaTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑CC‑CCCCACGAGATCGCCACGTCGCTATAACCGt                                                        >  2:6268188/1‑93 (MQ=255)

                                  aaaTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGt                                                        >  1:6080046/1‑93 (MQ=255)

                                    aTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGCTCGCCGCGTCGCTATAACCGtgc                                                      >  2:6937268/1‑93 (MQ=255)

                                    aTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGtgc                                                      <  2:7805686/93‑1 (MQ=255)

                                    aTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGtgc                                                      <  1:1179006/93‑1 (MQ=255)

                                    aTCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGtgc                                                      >  2:4963025/1‑93 (MQ=255)

                                     tCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGtgct                                                     >  1:6925126/1‑93 (MQ=255)

                                     tCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑GTTTTG‑‑G‑‑CCTCCCCACGAGATGGCCGGGTCGCTATAACCGtgcg                                                     >  2:6492197/1‑92 (MQ=255)

                                     accGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGtgct                                                     <  2:2570190/92‑1 (MQ=255)

                                         ttAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGt                                                 <  2:8890295/93‑1 (MQ=255)

                                          tAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGG‑TTTTCG‑‑C‑‑CC‑CCCCACGAGATCGGCGCGTCGCTATAACCGTGCTGCGTg                                                >  2:6665199/1‑93 (MQ=255)

                                          tAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTg                                                <  1:8407834/93‑1 (MQ=255)

                                          tAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTg                                                <  1:1305291/93‑1 (MQ=255)

                                           aaCCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGa                                               >  1:542269/1‑93 (MQ=255)

                                            aCCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAg                                              <  1:1606149/93‑1 (MQ=255)

                                            aCCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAg                                              <  2:7444145/93‑1 (MQ=255)

                                             ccGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAgg                                             <  2:9123530/93‑1 (MQ=255)

                                             ccGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAgg                                             <  2:1073034/93‑1 (MQ=255)

                                              cGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGgc                                            <  2:2827125/93‑1 (MQ=255)

                                                tCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGgcgc                                          >  1:892352/1‑93 (MQ=255)

                                                tCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGgcgc                                          <  2:8230813/93‑1 (MQ=255)

                                                tCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGgcgc                                          <  2:9537425/93‑1 (MQ=255)

                                                tCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGgcgc                                          <  1:4875409/93‑1 (MQ=255)

                                                 cTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTGCCC‑‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGgcgc                                          <  1:9019521/93‑1 (MQ=255)

                                                 cTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCt                                         <  1:4399262/93‑1 (MQ=255)

                                                  ttGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTg                                        >  1:2562155/1‑93 (MQ=255)

                                                  ttGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTg                                        >  2:7629988/1‑93 (MQ=255)

                                                   tGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTgg                                       >  1:6542054/1‑93 (MQ=255)

                                                   tGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTgg                                       <  2:5127810/93‑1 (MQ=255)

                                                   tGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTgg                                       >  2:6872453/1‑93 (MQ=255)

                                                     gTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCa                                     <  2:167503/93‑1 (MQ=255)

                                                     gTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCa                                     <  2:6085172/93‑1 (MQ=255)

                                                      ttATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCAt                                    <  2:1379335/93‑1 (MQ=255)

                                                        aTGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTg                                  >  1:2153055/1‑93 (MQ=255)

                                                        aTGGCGCCATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTg                                  <  1:5788492/93‑1 (MQ=255)

                                                          ggCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTggg                                >  1:995181/1‑93 (MQ=255)

                                                              cgATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTc                            >  2:8505885/1‑93 (MQ=255)

                                                              cgATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTc                            <  2:3240604/93‑1 (MQ=255)

                                                              cgATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTc                            <  1:4304192/93‑1 (MQ=255)

                                                                aTGCAACTGGCAGGTCCTGGA‑GTTTTTTG‑‑C‑‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCaa                          >  2:2617211/1‑93 (MQ=255)

                                                                aTGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCaa                          >  1:6424808/1‑93 (MQ=255)

                                                                 tGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAAt                         >  2:1054549/1‑93 (MQ=255)

                                                                 tGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAAt                         >  2:6802352/1‑93 (MQ=255)

                                                                  gCAACTGGCAGGTCCTGGA‑G‑TTTTTGC‑C‑‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATc                        <  1:8767379/93‑1 (MQ=255)

                                                                   cAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCa                       <  2:5684098/93‑1 (MQ=255)

                                                                    aaCTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCat                      <  1:3635534/93‑1 (MQ=255)

                                                                     aCTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCata                     <  2:1333500/93‑1 (MQ=255)

                                                                     aCTGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCata                     >  2:4450793/1‑93 (MQ=255)

                                                                       tGGCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATAtt                   >  1:6201473/1‑93 (MQ=255)

                                                                        ggCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTg                  <  1:5026568/93‑1 (MQ=255)

                                                                        ggCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTg                  <  1:7693417/93‑1 (MQ=255)

                                                                         gCAGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGa                 >  2:2103002/1‑93 (MQ=255)

                                                                           aGGTCCTGGA‑G‑TTTTTG‑‑GCCCC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGGCAATCATACTGAt                >  2:2931149/1‑93 (MQ=255)

                                                                           aGGTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATa               <  1:6857099/93‑1 (MQ=255)

                                                                            ggTCCTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATAc              >  2:6336910/1‑93 (MQ=255)

                                                                               ccTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAg           >  1:3956854/1‑93 (MQ=255)

                                                                                cTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGc          <  2:5491846/93‑1 (MQ=255)

                                                                                cTGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATAATATTGATACCAGc          >  2:4266126/1‑93 (MQ=255)

                                                                                 tGGA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCg         >  2:2392007/1‑93 (MQ=255)

                                                                                  ggA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGa        >  2:9378375/1‑93 (MQ=255)

                                                                                  ggA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGa        >  1:1132029/1‑93 (MQ=255)

                                                                                  ggA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGa        <  1:5092354/93‑1 (MQ=255)

                                                                                   gA‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGAc       >  1:435762/1‑93 (MQ=255)

                                                                                    a‑G‑TTTTTG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACt      >  1:79047/1‑93 (MQ=255)

                                                                                          tttG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTa  <  1:1929494/93‑1 (MQ=255)

                                                                                          tttG‑‑GC‑CC‑CCCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTa  <  2:420024/93‑1 (MQ=255)

TGCGATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGA‑G‑TTTTTG‑‑G‑‑CC‑CCCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTA  >  NC_000913/1472241‑1472420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑G‑‑CCC‑CCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGT  >  NC_000913/1472246‑1472432

TGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑G‑‑CCC‑CCC                                                                                               <  SRR4302540.3819026/94‑1 (MQ=60)

 gagtagcaatacagccacccgcgcgACAAAATCCGTTAACCGTCTGGTTTATGGCGCGATGCAACTGGCAGGTCCTGGACTTTTTG‑‑G‑‑CCC‑CCCA                                                                                              <  SRR4302540.7066244/69‑1 (MQ=60)

 GAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑G‑‑CCC‑CCCC                                                                                              <  SRR4302540.8150472/94‑1 (MQ=60)

  AGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑G‑‑CCC‑CCCca                                                                                             >  SRR4302540.7321996/1‑92 (MQ=60)

   GCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑G‑‑CCC‑CCCcac                                                                                            <  SRR4302540.8881814/94‑4 (MQ=60)

   GCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑G‑‑CCC‑CCCcac                                                                                            <  SRR4302540.4541970/94‑4 (MQ=60)

   GCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑G‑‑CCC‑CCCcac                                                                                            <  SRR4302540.5695555/94‑4 (MQ=60)

   GCAGCAATACATTTACTCTCGGTACAAAATCCGGTACCCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑G‑‑CCC‑CCCcac                                                                                            <  SRR4302540.1515470/94‑4 (MQ=60)

      GCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAG                                                                                          <  SRR4302540.7538980/94‑1 (MQ=60)

      GCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAG                                                                                          <  SRR4302540.7538981/94‑1 (MQ=60)

       CAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGA                                                                                         >  SRR4302540.611258/1‑94 (MQ=60)

        AATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGAT                                                                                        >  SRR4302540.4364129/1‑94 (MQ=60)

        AATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGAT                                                                                        >  SRR4302540.2771483/1‑94 (MQ=60)

        AATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGAT                                                                                        <  SRR4302540.3578561/94‑1 (MQ=60)

         ATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATC                                                                                       <  SRR4302540.9106964/94‑1 (MQ=60)

         ATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATC                                                                                       <  SRR4302540.3442023/94‑1 (MQ=60)

         ATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATC                                                                                       <  SRR4302540.4749266/94‑1 (MQ=60)

          TACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCG                                                                                      <  SRR4302540.463810/94‑1 (MQ=60)

          TACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCG                                                                                      >  SRR4302540.3883387/1‑94 (MQ=60)

          TACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCG                                                                                      >  SRR4302540.3883388/1‑94 (MQ=60)

            CATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCC                                                                                    <  SRR4302540.1538955/94‑1 (MQ=60)

               TTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCG                                                                                 >  SRR4302540.3426565/1‑94 (MQ=60)

               TTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCG                                                                                 <  SRR4302540.4499716/94‑1 (MQ=60)

               TTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCG                                                                                 <  SRR4302540.472600/94‑1 (MQ=60)

                TACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGT                                                                                >  SRR4302540.9099987/1‑94 (MQ=60)

                 ACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTC                                                                               <  SRR4302540.5842848/94‑1 (MQ=60)

                 ACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTC                                                                               <  SRR4302540.8701716/94‑1 (MQ=60)

                  CTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCG                                                                              >  SRR4302540.7985084/1‑94 (MQ=60)

                   TCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGC                                                                             >  SRR4302540.7814751/1‑94 (MQ=60)

                    CTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCT                                                                            <  SRR4302540.7098109/94‑1 (MQ=60)

                    CTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCT                                                                            >  SRR4302540.3089547/1‑94 (MQ=60)

                      CGGTACAAAATCCGTTAACCGTCTTGGTTTTGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑G‑‑CCCACCCACGATATCGCtgcatcgccat                                                                          >  SRR4302540.7748157/1‑83 (MQ=60)

                      CGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTAT                                                                          <  SRR4302540.1897207/94‑1 (MQ=60)

                       GGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GCCCCC‑CCCACGAGATCGCCGCGTCGCTAT                                                                          >  SRR4302540.3700903/1‑94 (MQ=60)

                       GGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGCCGCTATA                                                                         >  SRR4302540.7226791/1‑94 (MQ=60)

                       GGTACAAAATCCGTTAACCGTATTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATA                                                                         <  SRR4302540.5106293/94‑1 (MQ=60)

                        GTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAA                                                                        <  SRR4302540.3610739/94‑1 (MQ=60)

                         TACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GA‑CCC‑CCCACGAGATCGCCGCGTCGCTATAAC                                                                       >  SRR4302540.9268144/1‑94 (MQ=60)

                         TACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGCCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGAGCGCCGCTTCGCTATAAC                                                                       >  SRR4302540.3376405/1‑94 (MQ=60)

                            AAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGT                                                                    >  SRR4302540.6147659/1‑94 (MQ=60)

                               ATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCT                                                                 >  SRR4302540.7002196/1‑94 (MQ=60)

                               ATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCT                                                                 <  SRR4302540.1191187/94‑1 (MQ=60)

                               ATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCT                                                                 >  SRR4302540.4101662/1‑94 (MQ=60)

                                TCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTG                                                                >  SRR4302540.3732105/1‑94 (MQ=60)

                                 CCGTTAGCCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑G‑‑CCC‑CCCACGAGATCGCCGCCTCGCTATAACCGTGCTGCG                                                              <  SRR4302540.8305240/94‑1 (MQ=60)

                                 CCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGC                                                               >  SRR4302540.9321757/1‑94 (MQ=60)

                                  CGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCG                                                              <  SRR4302540.4026368/94‑1 (MQ=60)

                                     TAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GCCCCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTG                                                            <  SRR4302540.4697354/94‑1 (MQ=60)

                                     TAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGA                                                           <  SRR4302540.622942/94‑1 (MQ=60)

                                     TAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGA                                                           >  SRR4302540.548134/1‑94 (MQ=60)

                                     TAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGA                                                           <  SRR4302540.1318831/94‑1 (MQ=60)

                                     TAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGA                                                           <  SRR4302540.8505422/94‑1 (MQ=60)

                                      AACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAG                                                          <  SRR4302540.1843671/94‑1 (MQ=60)

                                       ACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GCCCCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAG                                                          <  SRR4302540.1612414/94‑1 (MQ=60)

                                       ACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GCCCCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAG                                                          <  SRR4302540.6280474/94‑1 (MQ=60)

                                       ACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGG                                                         <  SRR4302540.10003804/94‑1 (MQ=60)

                                       ACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGG                                                         <  SRR4302540.1623729/94‑1 (MQ=60)

                                        CCGTCTTGATTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCTCGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGC                                                        <  SRR4302540.5591654/94‑1 (MQ=60)

                                         CGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCG                                                       <  SRR4302540.6969920/94‑1 (MQ=60)

                                         CGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCG                                                       >  SRR4302540.6586523/1‑94 (MQ=60)

                                          GTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGC                                                      >  SRR4302540.901835/1‑94 (MQ=60)

                                           TCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCT                                                     <  SRR4302540.4927734/94‑1 (MQ=60)

                                            CTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTGCCC‑‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCT                                                     <  SRR4302540.9127751/94‑1 (MQ=60)

                                            CTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTG                                                    >  SRR4302540.2589778/1‑94 (MQ=60)

                                            CTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTG                                                    >  SRR4302540.1498883/1‑94 (MQ=60)

                                            CTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTG                                                    >  SRR4302540.2589777/1‑94 (MQ=60)

                                            CTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTG                                                    <  SRR4302540.4446976/94‑1 (MQ=60)

                                             TTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGG                                                   >  SRR4302540.6614651/1‑94 (MQ=60)

                                                GTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCAT                                                <  SRR4302540.1274072/94‑1 (MQ=60)

                                                  TATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTG                                              >  SRR4302540.2176215/1‑94 (MQ=60)

                                                   ATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGG                                             <  SRR4302540.5512835/94‑1 (MQ=60)

                                                   ATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGG                                             <  SRR4302540.9780177/94‑1 (MQ=60)

                                                   ATGGCGCCATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGG                                             <  SRR4302540.5852446/94‑1 (MQ=60)

                                                    TGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGG                                            >  SRR4302540.1005314/1‑94 (MQ=60)

                                                     GGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGC                                           <  SRR4302540.10080896/94‑1 (MQ=60)

                                                        GCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTC                                        <  SRR4302540.5913297/94‑1 (MQ=60)

                                                        GCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑G‑‑CCC‑CCCcaaaggatcgcccggccgctataacacggctgcgcgggcgccggcatttagggaca                                       >  SRR4302540.8490773/1‑38 (MQ=60)

                                                         CGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCA                                       <  SRR4302540.4351039/94‑1 (MQ=60)

                                                          GATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAA                                      >  SRR4302540.6496319/1‑94 (MQ=60)

                                                           ATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAAT                                     <  SRR4302540.8054338/94‑1 (MQ=60)

                                                             GCAACTGGCAGGTCCTGGAGTTTTTGC‑C‑‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCA                                   <  SRR4302540.8869003/94‑1 (MQ=60)

                                                              CAACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCGCGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCAT                                  <  SRR4302540.6895025/94‑1 (MQ=60)

                                                               AACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATA                                 <  SRR4302540.5016634/94‑1 (MQ=60)

                                                               AACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATA                                 <  SRR4302540.3675331/94‑1 (MQ=60)

                                                               AACTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATA                                 <  SRR4302540.4441616/94‑1 (MQ=60)

                                                                 CTGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATT                               >  SRR4302540.6270504/1‑94 (MQ=60)

                                                                  TGGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTG                              >  SRR4302540.5241248/1‑94 (MQ=60)

                                                                   GGCAGGTGCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGA                             <  SRR4302540.7780991/94‑1 (MQ=60)

                                                                   GGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGA                             <  SRR4302540.7780990/94‑1 (MQ=60)

                                                                   GGCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGA                             <  SRR4302540.5080973/94‑1 (MQ=60)

                                                                    GCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGAT                            <  SRR4302540.1526326/94‑1 (MQ=60)

                                                                    GCAGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGAT                            <  SRR4302540.2301743/94‑1 (MQ=60)

                                                                      AGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATAC                          <  SRR4302540.4970194/94‑1 (MQ=60)

                                                                      AGGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATAC                          <  SRR4302540.6933078/94‑1 (MQ=60)

                                                                       GGTCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACC                         >  SRR4302540.8791230/1‑94 (MQ=60)

                                                                         TCCTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAG                       >  SRR4302540.3999545/1‑94 (MQ=60)

                                                                           CTGGAGTTTTTG‑‑GCCCCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGC                      >  SRR4302540.4521238/1‑94 (MQ=60)

                                                                           CTGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCG                     <  SRR4302540.4572776/94‑1 (MQ=60)

                                                                            TGGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGA                    >  SRR4302540.1143814/1‑94 (MQ=60)

                                                                             GGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGAC                   <  SRR4302540.5147443/94‑1 (MQ=60)

                                                                             GGAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGAC                   >  SRR4302540.440380/1‑94 (MQ=60)

                                                                              GAGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACT                  >  SRR4302540.79875/1‑94 (MQ=60)

                                                                               AGTTTTTGC‑C‑‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTT                 >  SRR4302540.8783334/1‑94 (MQ=60)

                                                                               AGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTT                 <  SRR4302540.6742384/94‑1 (MQ=60)

                                                                               AGTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTT                 <  SRR4302540.200644/94‑1 (MQ=60)

                                                                                GTTTTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTT                <  SRR4302540.1362013/94‑1 (MQ=60)

                                                                                   TTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTAT             <  SRR4302540.1145676/94‑1 (MQ=60)

                                                                                   TTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTAT             <  SRR4302540.5700934/94‑1 (MQ=60)

                                                                                   TTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTAT             <  SRR4302540.8556771/94‑1 (MQ=60)

                                                                                   TTTG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTAT             <  SRR4302540.1950522/94‑1 (MQ=60)

                                                                                    TTG‑‑GCCCCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTAT             <  SRR4302540.4113120/94‑1 (MQ=60)

                                                                                     TG‑‑GC‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGG           <  SRR4302540.9452422/94‑1 (MQ=60)

                                                                                      G‑‑C‑‑CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTC         >  SRR4302540.7744227/1‑94 (MQ=60)

                                                                                            CCC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCG       <  SRR4302540.6155429/94‑1 (MQ=60)

                                                                                             CC‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGC      <  SRR4302540.1652110/94‑1 (MQ=60)

                                                                                              C‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCA     <  SRR4302540.5273597/94‑1 (MQ=60)

                                                                                              C‑CCCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCA     >  SRR4302540.2720474/1‑94 (MQ=60)

                                                                                                 CCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACG   <  SRR4302540.1681934/94‑1 (MQ=60)

                                                                                                 CCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACG   <  SRR4302540.1625072/94‑1 (MQ=60)

                                                                                                 CCACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACG   <  SRR4302540.1309622/94‑1 (MQ=60)

                                                                                                  CACGAGATCGCCGCGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGT  >  SRR4302540.2091549/1‑94 (MQ=60)

TGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCGATGCAACTGGCAGGTCCTGGAGTTTTTG‑‑G‑‑CCC‑CCCACGAGATCGCCACGTCGCTATAACCGTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGT  >  NC_000913/1472246‑1472432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`