Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,690,777 T→C L29L (CTA→CTG)  uidC ← membrane‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,690,7770TC100.0% 503.4 / NA 157L29L (CTA→CTG) uidCmembrane‑associated protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (69/88);  total (69/88)

Only 100 of 164 total aligned reads displayed.

AATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTAT  >  NC_000913/1690688‑1690866
                                                                                         |                                                                                         
  tcctccCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTcc                                                                                      >  2:3297198/1‑93 (MQ=255)
  tcctccCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTCTTTCAGTCTGATGCGCAGTcc                                                                                      <  2:4007676/93‑1 (MQ=255)
   cctccCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTccc                                                                                     >  2:3178101/1‑93 (MQ=255)
   cctccCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTccc                                                                                     >  1:240760/1‑93 (MQ=255)
    ctccCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCg                                                                                    >  1:4293869/1‑93 (MQ=255)
     tccCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGc                                                                                   >  2:3408003/1‑93 (MQ=255)
      cccTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCt                                                                                  <  2:4136928/93‑1 (MQ=255)
      cccTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCt                                                                                  >  2:4350366/1‑93 (MQ=255)
       ccTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCtt                                                                                 >  1:1194649/1‑93 (MQ=255)
        cTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTc                                                                                <  2:5720900/93‑1 (MQ=255)
        cTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTc                                                                                >  1:3158577/1‑93 (MQ=255)
          gTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCAt                                                                              <  1:264966/93‑1 (MQ=255)
           tACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATc                                                                             >  1:2146273/1‑93 (MQ=255)
           tACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATc                                                                             >  2:2641266/1‑93 (MQ=255)
           tACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATc                                                                             <  2:2117494/93‑1 (MQ=255)
             cccATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGt                                                                           <  1:913057/93‑1 (MQ=255)
             cccATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGt                                                                           >  1:3338876/1‑93 (MQ=255)
             cccATGCGTAAATATCTCTTCCCGCGCCAGCACTGAGCTTATCCACCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTGCCGCTTCATCGt                                                                           >  2:3972683/1‑93 (MQ=255)
               cATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCa                                                                         <  1:3347004/93‑1 (MQ=255)
               cATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCa                                                                         <  1:5468646/93‑1 (MQ=255)
               cATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCa                                                                         >  1:5285171/1‑93 (MQ=255)
               cATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCa                                                                         >  2:3322382/1‑93 (MQ=255)
                    gTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAg                                                                    >  2:6167084/1‑93 (MQ=255)
                    gTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAg                                                                    >  2:5451100/1‑93 (MQ=255)
                    gTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAg                                                                    <  2:5488768/93‑1 (MQ=255)
                      aaaTATCTCTTCCCGCGCCAGCATTGGGCTTATCCACCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGtt                                                                  <  2:4720279/93‑1 (MQ=255)
                      aaaTATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGtt                                                                  <  2:3074649/93‑1 (MQ=255)
                      aaaTATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGtt                                                                  <  1:6165450/93‑1 (MQ=255)
                      aaaTATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGtt                                                                  >  2:5350796/1‑93 (MQ=255)
                      aaaTATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGtt                                                                  <  2:382708/93‑1 (MQ=255)
                        atatCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTgc                                                                <  2:6290642/93‑1 (MQ=255)
                         tatCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTgcg                                                               >  1:45997/1‑93 (MQ=255)
                          atCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTgcgc                                                              <  1:725028/93‑1 (MQ=255)
                            ctctTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGcgccg                                                            >  2:579661/1‑93 (MQ=255)
                               ttCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCAt                                                         >  1:1942639/1‑93 (MQ=255)
                                tCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATa                                                        <  1:4524297/93‑1 (MQ=255)
                                tCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATa                                                        <  1:5962058/93‑1 (MQ=255)
                                tCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATa                                                        <  1:4310678/93‑1 (MQ=255)
                                  ccGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAg                                                      >  1:1380858/1‑93 (MQ=255)
                                  ccGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAg                                                      >  1:1349165/1‑93 (MQ=255)
                                    gcgcCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGTTTCATCGTCAGCCAGTTGCGCCGCATATGct                                                    >  1:4840640/1‑92 (MQ=255)
                                    gcgcCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCa                                                    >  2:1447340/1‑93 (MQ=255)
                                    gcgcCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCa                                                    <  1:6086310/93‑1 (MQ=255)
                                      gcCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCaga                                                  <  1:116148/93‑1 (MQ=255)
                                        cAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAgg                                                <  1:5144704/93‑1 (MQ=255)
                                        cAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAgg                                                <  1:4196747/93‑1 (MQ=255)
                                            aCTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAg                                            >  1:3248057/1‑93 (MQ=255)
                                               gggCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGcc                                         <  1:1510961/93‑1 (MQ=255)
                                               gggCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGcc                                         <  2:1747131/93‑1 (MQ=255)
                                                ggCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCa                                        <  2:502658/93‑1 (MQ=255)
                                                ggCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCa                                        >  1:4541348/1‑93 (MQ=255)
                                                 gCTTATCCACCCTGCGCAATTCGTTTTTCAGTCTGCCGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGGAAAGCCag                                       >  2:2769652/1‑93 (MQ=255)
                                                     aTCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGc                                   >  2:368027/1‑93 (MQ=255)
                                                     aTCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGc                                   <  1:3715184/93‑1 (MQ=255)
                                                     aTCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGc                                   <  2:286664/93‑1 (MQ=255)
                                                      tCCGCCCTGCGCAATTCGTTTGTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCa                                  <  2:2523923/93‑1 (MQ=255)
                                                        cGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGc                                <  2:1024405/93‑1 (MQ=255)
                                                        cGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGc                                <  1:211746/93‑1 (MQ=255)
                                                        cGCCCTGCGCAATTCGTTTTTCAGTCTGAGGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGc                                <  2:3002321/93‑1 (MQ=255)
                                                         gCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGcc                               <  2:1900007/93‑1 (MQ=255)
                                                           ccTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGt                             >  2:4883555/1‑93 (MQ=255)
                                                           ccTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGt                             >  1:393721/1‑93 (MQ=255)
                                                            cTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTc                            <  2:4363475/93‑1 (MQ=255)
                                                            cTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTc                            <  2:5994940/93‑1 (MQ=255)
                                                             tGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCa                           <  1:2521064/93‑1 (MQ=255)
                                                             tGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCa                           >  1:3693264/1‑93 (MQ=255)
                                                             tGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCa                           >  2:1006028/1‑93 (MQ=255)
                                                             tGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCa                           <  2:3508981/93‑1 (MQ=255)
                                                                gcAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGc                        <  1:4097019/93‑1 (MQ=255)
                                                                  aaTTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCaa                      >  2:8112/1‑93 (MQ=255)
                                                                  aaTTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCaa                      >  2:3653007/1‑93 (MQ=255)
                                                                  aaTTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCaa                      >  1:2480422/1‑93 (MQ=255)
                                                                  aaTTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCaa                      >  2:4775707/1‑93 (MQ=255)
                                                                   aTTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCaaa                     <  2:1275631/93‑1 (MQ=255)
                                                                   aTTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCaaa                     <  1:4947026/93‑1 (MQ=255)
                                                                    ttCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAAt                    >  2:3548977/1‑93 (MQ=255)
                                                                     tCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATa                   <  1:2920773/93‑1 (MQ=255)
                                                                      cGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATaa                  <  2:3066402/93‑1 (MQ=255)
                                                                      cGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATaa                  <  2:2906030/93‑1 (MQ=255)
                                                                      cGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATaa                  <  1:6146781/93‑1 (MQ=255)
                                                                        tttttCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACg                <  2:2639812/93‑1 (MQ=255)
                                                                          tttCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGc              >  1:3956603/1‑93 (MQ=255)
                                                                           ttCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGcc             >  2:924011/1‑93 (MQ=255)
                                                                            tCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCGGAGGCAGCCGTCAGGCAAATAACGGCCa            <  2:1063716/93‑1 (MQ=255)
                                                                            tCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCa            <  1:1751493/93‑1 (MQ=255)
                                                                            tCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCa            <  1:2218995/93‑1 (MQ=255)
                                                                            tCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCa            <  1:5197861/93‑1 (MQ=255)
                                                                            tCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCa            >  2:2041903/1‑93 (MQ=255)
                                                                             cAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCAt           <  1:3552954/93‑1 (MQ=255)
                                                                             cAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCAt           <  1:2899394/93‑1 (MQ=255)
                                                                              aGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATg          <  2:1795544/93‑1 (MQ=255)
                                                                              aGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATg          >  1:3072570/1‑93 (MQ=255)
                                                                              aGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATg          <  2:2524794/93‑1 (MQ=255)
                                                                                tCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGc        >  2:4996373/1‑93 (MQ=255)
                                                                                tCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGc        <  2:2442926/93‑1 (MQ=255)
                                                                                 cTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGcc       <  2:1627253/93‑1 (MQ=255)
                                                                                   gATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCAc     >  2:1397996/1‑93 (MQ=255)
                                                                                   gATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCAc     <  1:6295329/93‑1 (MQ=255)
                                                                                      gcgcAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTAt  <  2:1258401/93‑1 (MQ=255)
                                                                                      gcgcAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTAt  <  1:5460282/93‑1 (MQ=255)
                                                                                         |                                                                                         
AATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTAT  >  NC_000913/1690688‑1690866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTT  >  NC_000913/1690685‑1690870
                                                                                            |                                                                                             
AGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCA                                                                                              >  SRR4302533.1210512/1‑94 (MQ=60)
 GCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAG                                                                                             <  SRR4302533.278513/94‑1 (MQ=60)
     TCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCC                                                                                         >  SRR4302533.242254/1‑94 (MQ=60)
      CCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCG                                                                                        >  SRR4302533.4319694/1‑94 (MQ=60)
         CCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTT                                                                                     >  SRR4302533.1201829/1‑94 (MQ=60)
          CCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTC                                                                                    >  SRR4302533.3178015/1‑94 (MQ=60)
             GTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATC                                                                                 <  SRR4302533.4834773/94‑1 (MQ=60)
             GTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATC                                                                                 >  SRR4302533.2159492/1‑94 (MQ=60)
             GTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATC                                                                                 <  SRR4302533.266578/94‑1 (MQ=60)
              TACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCG                                                                                >  SRR4302533.1965536/1‑94 (MQ=60)
               ACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGT                                                                               >  SRR4302533.3359511/1‑94 (MQ=60)
               ACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGT                                                                               >  SRR4302533.6188533/1‑94 (MQ=60)
                CCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTC                                                                              <  SRR4302533.918419/94‑1 (MQ=60)
                 CCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCA                                                                             >  SRR4302533.5316829/1‑94 (MQ=60)
                  CATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAG                                                                            <  SRR4302533.5501606/94‑1 (MQ=60)
                  CATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAG                                                                            >  SRR4302533.6097074/1‑94 (MQ=60)
                  CATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAG                                                                            <  SRR4302533.3367684/94‑1 (MQ=60)
                         AAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTG                                                                     <  SRR4302533.2552497/94‑1 (MQ=60)
                         AAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTG                                                                     <  SRR4302533.6202511/94‑1 (MQ=60)
                           ATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCG                                                                   >  SRR4302533.46314/1‑94 (MQ=60)
                           ATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCG                                                                   >  SRR4302533.46316/1‑94 (MQ=60)
                            TATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGGGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGGTGCGC                                                                  >  SRR4302533.5157603/1‑94 (MQ=60)
                             ATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCC                                                                 <  SRR4302533.729316/94‑1 (MQ=60)
                             ATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCC                                                                 <  SRR4302533.1254322/94‑1 (MQ=60)
                                 CTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCAT                                                             >  SRR4302533.1954491/1‑94 (MQ=60)
                                   TCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAA                                                           <  SRR4302533.4551686/94‑1 (MQ=60)
                                   TCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAA                                                           <  SRR4302533.5998128/94‑1 (MQ=60)
                                   TCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAA                                                           <  SRR4302533.3854179/94‑1 (MQ=60)
                                   TCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAA                                                           <  SRR4302533.4336627/94‑1 (MQ=60)
                                   TCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAA                                                           <  SRR4302533.3708617/94‑1 (MQ=60)
                                    CCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAG                                                          >  SRR4302533.3385073/1‑94 (MQ=60)
                                    CCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAG                                                          >  SRR4302533.748381/1‑94 (MQ=60)
                                    CCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAG                                                          >  SRR4302533.1389172/1‑94 (MQ=60)
                                    CCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAG                                                          >  SRR4302533.1357282/1‑94 (MQ=60)
                                      CGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGTTTCATCGTCAGCCAGTTGCGCCGCATAtgct                                                        >  SRR4302533.4869979/1‑90 (MQ=60)
                                       GCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAG                                                       <  SRR4302533.6123105/94‑1 (MQ=60)
                                         GCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAG                                                     <  SRR4302533.116887/94‑1 (MQ=60)
                                          CCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGG                                                    >  SRR4302533.4370451/1‑94 (MQ=60)
                                           CAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGT                                                   <  SRR4302533.4222154/94‑1 (MQ=60)
                                           CAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGT                                                   <  SRR4302533.5175793/94‑1 (MQ=60)
                                            AGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTA                                                  >  SRR4302533.5895845/1‑94 (MQ=60)
                                            AGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTA                                                  <  SRR4302533.3967927/94‑1 (MQ=60)
                                              CACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAG                                                >  SRR4302533.3268086/1‑94 (MQ=60)
                                              CACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAG                                                <  SRR4302533.3062723/94‑1 (MQ=60)
                                              CACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAG                                                <  SRR4302533.159644/94‑1 (MQ=60)
                                              CACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAG                                                <  SRR4302533.4155534/94‑1 (MQ=60)
                                                CTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGC                                              >  SRR4302533.6245986/1‑94 (MQ=60)
                                                  GGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCA                                            >  SRR4302533.4568849/1‑94 (MQ=60)
                                                  GGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCA                                            >  SRR4302533.2439614/1‑94 (MQ=60)
                                                  GGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCA                                            <  SRR4302533.1520067/94‑1 (MQ=60)
                                                        ATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCA                                      <  SRR4302533.3737990/94‑1 (MQ=60)
                                                           CGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCC                                   <  SRR4302533.213080/94‑1 (MQ=60)
                                                            GCCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCG                                  >  SRR4302533.4320697/1‑94 (MQ=60)
                                                             CCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGT                                 >  SRR4302533.396108/1‑94 (MQ=60)
                                                             CCCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGT                                 >  SRR4302533.325812/1‑94 (MQ=60)
                                                              CCTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTC                                <  SRR4302533.2289598/94‑1 (MQ=60)
                                                               CTGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCA                               >  SRR4302533.3715952/1‑94 (MQ=60)
                                                                TGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAG                              <  SRR4302533.2536598/94‑1 (MQ=60)
                                                                TGCGCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAG                              <  SRR4302533.3274995/94‑1 (MQ=60)
                                                                   GCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCA                           <  SRR4302533.4121960/94‑1 (MQ=60)
                                                                   GCAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCA                           <  SRR4302533.5228125/94‑1 (MQ=60)
                                                                    CAATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAA                          >  SRR4302533.2495718/1‑94 (MQ=60)
                                                                      ATTCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAAT                        <  SRR4302533.4976971/94‑1 (MQ=60)
                                                                        TCGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAA                      <  SRR4302533.2938665/94‑1 (MQ=60)
                                                                         CGTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAAC                     <  SRR4302533.6183786/94‑1 (MQ=60)
                                                                          GTTTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCAGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACG                    <  SRR4302533.1499948/94‑1 (MQ=60)
                                                                            TTTTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGC                  >  SRR4302533.3980720/1‑94 (MQ=60)
                                                                              TTCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCA                <  SRR4302533.2923634/94‑1 (MQ=60)
                                                                               TCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCAT               <  SRR4302533.2232694/94‑1 (MQ=60)
                                                                               TCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCAT               <  SRR4302533.5326234/94‑1 (MQ=60)
                                                                               TCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCAT               <  SRR4302533.1762157/94‑1 (MQ=60)
                                                                               TCAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCAT               <  SRR4302533.5229208/94‑1 (MQ=60)
                                                                                CAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATG              <  SRR4302533.2917212/94‑1 (MQ=60)
                                                                                CAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATG              >  SRR4302533.3091432/1‑94 (MQ=60)
                                                                                CAGTCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATG              <  SRR4302533.3574838/94‑1 (MQ=60)
                                                                                   TCTGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCC           <  SRR4302533.935364/94‑1 (MQ=60)
                                                                                     TGATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCAC         <  SRR4302533.2143373/94‑1 (MQ=60)
                                                                                      GATGCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACT        <  SRR4302533.6332867/94‑1 (MQ=60)
                                                                                         GCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATT     <  SRR4302533.5493178/94‑1 (MQ=60)
                                                                                         GCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATT     >  SRR4302533.2940045/1‑94 (MQ=60)
                                                                                         GCGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGACATGGCCACTATT     >  SRR4302533.2940044/1‑94 (MQ=60)
                                                                                          CGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTT    >  SRR4302533.5773306/1‑94 (MQ=60)
                                                                                          CGCAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTT    >  SRR4302533.4166065/1‑94 (MQ=60)
                                                                                            CAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTT  <  SRR4302533.3075078/94‑1 (MQ=60)
                                                                                            |                                                                                             
AGCAATCCTCCCTGTACCCATGCGTAAATATCTCTTCCCGCGCCAGCACTGGGCTTATCCGCCCTGCGCAATTCGTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTT  >  NC_000913/1690685‑1690870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑