Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,691,451 T→C A279A (GCA→GCG)  uidB ← glucuronide permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,691,4510TC89.7% 479.6 / 42.0 195A279A (GCA→GCG) uidBglucuronide permease
Reads supporting (aligned to +/- strand):  ref base T (7/13);  new base C (97/78);  total (104/91)
Fisher's exact test for biased strand distribution p-value = 9.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.20e-02

Only 100 of 204 total aligned reads displayed.

GATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGG  >  NC_000913/1691363‑1691542
                                                                                        |                                                                                           
aaaaaCCGCAGGTCCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGCCCACCCCCGGCACCAGCGGTGCCGACGCca                                                                                         <  1:7383808/90‑1 (MQ=255)
   aaCCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAg                                                                                      <  1:6566806/93‑1 (MQ=255)
   aaCCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAg                                                                                      <  2:3300788/93‑1 (MQ=255)
    aCCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGt                                                                                     <  2:3637996/93‑1 (MQ=255)
     ccGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTa                                                                                    <  1:6013933/93‑1 (MQ=255)
      cGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTac                                                                                   >  2:1259713/1‑93 (MQ=255)
      cGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACCGTac                                                                                   >  2:4801110/1‑93 (MQ=255)
       gCAGGTTCCCAGGAAAGGCCAAATCAGGAAGGTATTCTTTTTACCGATACTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTacc                                                                                  <  1:3286203/93‑1 (MQ=255)
       gCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTacc                                                                                  >  2:1698734/1‑93 (MQ=255)
        cAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTacca                                                                                 >  1:9443385/1‑93 (MQ=255)
         aGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTaccaa                                                                                <  2:3427057/93‑1 (MQ=255)
           gTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTaccaacc                                                                              <  1:274250/93‑1 (MQ=255)
           gTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTaccaacc                                                                              >  2:6513286/1‑93 (MQ=255)
             tCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAg                                                                            >  2:8350096/1‑93 (MQ=255)
             tCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAg                                                                            >  1:9961982/1‑93 (MQ=255)
               ccAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGt                                                                          >  1:252411/1‑93 (MQ=255)
               ccAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGt                                                                          <  2:6834282/93‑1 (MQ=255)
                 aGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTAACAACCAGGttt                                                                        >  2:4175783/1‑93 (MQ=255)
                  gCAAAGCGCCAATCGGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCACGGGACCCGCGACGACGACGCCACAGTACCAAACAGGtttt                                                                       >  2:6897863/1‑93 (MQ=255)
                  gCAAAGCGCCAATCAGGAAGCTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGtttt                                                                       >  1:1163973/1‑93 (MQ=255)
                   cAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTg                                                                      >  1:314050/1‑93 (MQ=255)
                   cAAAGCGCCAAGCAGGAAGGTATTTTTTTTACTAATCGTCGCGACCACGCCCGGCCCGATCGTTGCCGACGCCACAGTACCAACCAGGTTTTg                                                                      <  2:5014247/93‑1 (MQ=255)
                    aaaGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGGTTTGc                                                                     >  1:1971938/1‑93 (MQ=255)
                    aaaGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGCTTTGc                                                                     >  1:7642481/1‑93 (MQ=255)
                     aaGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCa                                                                    >  1:5020630/1‑93 (MQ=255)
                     aaGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCa                                                                    >  2:9434629/1‑93 (MQ=255)
                       gccccAATCAGGAAGTTATTCTTTTTCCCGACCCCCGCGACCACCCCCGGCACCGGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCAcc                                                                  <  2:261061/90‑1 (MQ=255)
                          ccAATCAGTAAGTTATTTTTTTTCCCGATCCCCGCGACCATCCCCGGCCCCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGt                                                               <  2:7986616/93‑1 (MQ=255)
                          ccAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGt                                                               <  2:6107509/93‑1 (MQ=255)
                          ccAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCCGCCCCAGCGGGGCCGTCGCCACGGTACCAACCAGGTTTTCCACCAGt                                                               >  1:7577575/1‑93 (MQ=255)
                            aaTCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGGCGACGCCACAGTACCAACCAGGCTTTGCACCAGTAc                                                             >  1:958949/1‑93 (MQ=255)
                            aaTCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTAc                                                             >  2:5086826/1‑93 (MQ=255)
                              tCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCa                                                           >  1:1186331/1‑93 (MQ=255)
                              tCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCa                                                           >  1:8564036/1‑93 (MQ=255)
                              tCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCa                                                           <  1:641050/93‑1 (MQ=255)
                              tCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCa                                                           >  2:8876167/1‑93 (MQ=255)
                                aGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCaccagcaccagc                                                         >  2:4121214/1‑93 (MQ=255)
                                aGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGc                                                         >  2:3161316/1‑93 (MQ=255)
                                  gAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGcac                                                       >  2:671032/1‑93 (MQ=255)
                                    aGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCCCCACCCGTGCCGACGCCCCAGTACCGACCAGGTTTTGCAACACTACCACCACAg                                                     >  1:3878472/1‑93 (MQ=255)
                                    aGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAg                                                     <  1:6296270/93‑1 (MQ=255)
                                     ggTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGt                                                    >  2:7558972/1‑93 (MQ=255)
                                     ggTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTCGCACCAGTACCAGCACAGt                                                    >  1:4149238/1‑93 (MQ=255)
                                      gTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTg                                                   >  1:7168702/1‑93 (MQ=255)
                                      gTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTg                                                   >  2:9514324/1‑93 (MQ=255)
                                      gTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGGGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTg                                                   >  1:8209988/1‑93 (MQ=255)
                                       tATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGa                                                  <  2:2101391/93‑1 (MQ=255)
                                       tATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGa                                                  >  2:1951885/1‑93 (MQ=255)
                                        aTTCTTTTTACCGACCCTCGCGACCACCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGaa                                                 <  1:7863906/93‑1 (MQ=255)
                                         ttCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAAc                                                >  1:4629905/1‑93 (MQ=255)
                                         ttCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAAc                                                <  2:8521603/93‑1 (MQ=255)
                                         ttCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAAc                                                <  1:1299843/93‑1 (MQ=255)
                                         ttCTTTTTACCGACCCTCGCGACCATCCCCGGCCCCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAAc                                                <  2:8015897/93‑1 (MQ=255)
                                            tttttACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGGTTTGCACCAGTACCAGCACAGTGAACaac                                             >  2:8274180/1‑91 (MQ=255)
                                                tACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCgg                                         <  2:9358366/93‑1 (MQ=255)
                                                 aCCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGt                                        >  1:5687624/1‑93 (MQ=255)
                                                 aCCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGt                                        >  1:8041933/1‑93 (MQ=255)
                                                 aCCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGCGAACAGCCCgga                                        >  2:9061746/1‑92 (MQ=255)
                                                  ccGATCCTCGCGACCATCCCCGGCACCAGCGGGGCCGACGCCACAGTACCAACCAGGGTTTGCACCAGTACCAGCACAGTGAACAGCCCggga                                       >  1:9031905/1‑91 (MQ=255)
                                                   cGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGGACAATGAACAACCCGGTAt                                      >  2:8084235/1‑93 (MQ=255)
                                                   cGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTAt                                      <  2:4406262/93‑1 (MQ=255)
                                                   cGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTAt                                      >  2:9625119/1‑93 (MQ=255)
                                                   cGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTAt                                      >  2:2192872/1‑93 (MQ=255)
                                                      tCCTCGCGCCCATCCCCGGCCCCAGCGGTCCCGACGCCCCAGTACCAGCCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAt                                   <  1:4955737/93‑1 (MQ=255)
                                                      tCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAt                                   >  1:7773469/1‑93 (MQ=255)
                                                      tCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAt                                   <  2:5364013/93‑1 (MQ=255)
                                                      tCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAt                                   >  1:3778008/1‑93 (MQ=255)
                                                      tCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAt                                   >  2:8100902/1‑93 (MQ=255)
                                                        cTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAttt                                 >  2:3799908/1‑93 (MQ=255)
                                                        cTCGCGACCACCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACATCCCGGTATCAttt                                 <  2:3203247/93‑1 (MQ=255)
                                                         tCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTa                                >  1:8559402/1‑93 (MQ=255)
                                                         tCGCGACCATCCCCGGCACCAGCGGGGCCGACGCCACAGAACCAACCAGGTTTGGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATCTa                                >  2:8972427/1‑93 (MQ=255)
                                                          cgcgACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTaa                               >  2:7578744/1‑93 (MQ=255)
                                                               ccATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACAt                          >  2:8798597/1‑93 (MQ=255)
                                                                cccccccGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATa                         <  1:1084479/90‑1 (MQ=255)
                                                                cATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCTCAGTGAACAGCACGGTATCATTTAACACATa                         >  2:3365124/1‑93 (MQ=255)
                                                                cATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATa                         <  2:2817240/93‑1 (MQ=255)
                                                                cATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATa                         >  1:6328913/1‑93 (MQ=255)
                                                                 aTCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGACCAGCCCGGTATCATTTAACACATAg                        >  1:413348/1‑93 (MQ=255)
                                                                 aTCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAg                        >  1:36208/1‑93 (MQ=255)
                                                                 aTCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAg                        >  2:7069753/1‑93 (MQ=255)
                                                                  tCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAgc                       >  1:8560753/1‑93 (MQ=255)
                                                                    cccGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAgcgc                     <  2:1276615/93‑1 (MQ=255)
                                                                     ccGGCACCAGTGGTGCCGACGCCACAGTACCAACCAGGGTTTGCACCAGTACCAGCACAGTGAACAGCCCGGGATCATTTAACACATAGCGCa                    >  2:9239861/1‑93 (MQ=255)
                                                                     ccGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCa                    <  2:9777371/93‑1 (MQ=255)
                                                                      cGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCAc                   >  2:5838015/1‑93 (MQ=255)
                                                                       ggCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACg                  <  1:8768176/93‑1 (MQ=255)
                                                                           ccAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGa              <  1:8148504/93‑1 (MQ=255)
                                                                           ccAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGa              <  2:7880996/93‑1 (MQ=255)
                                                                           ccAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGa              >  2:6044522/1‑93 (MQ=255)
                                                                            cAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaa             <  1:3251308/93‑1 (MQ=255)
                                                                            cAGCGGTGCCGACGCCACAGTACCAACCAGGTTTCGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaa             <  1:1013533/93‑1 (MQ=255)
                                                                             aGCGGGGCCGACGCCACGGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaac            <  1:7718889/93‑1 (MQ=255)
                                                                               cGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaacaa          >  1:2056323/1‑93 (MQ=255)
                                                                               cGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaacaa          <  1:1574628/93‑1 (MQ=255)
                                                                                ggTCCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaacaac         <  2:408287/93‑1 (MQ=255)
                                                                                 gTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAacg        <  1:6165969/93‑1 (MQ=255)
                                                                                   gCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAacgac      <  1:356611/93‑1 (MQ=255)
                                                                                     cGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAAcgacga    <  1:529100/93‑1 (MQ=255)
                                                                                     cGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAAcgacga    <  2:4041538/93‑1 (MQ=255)
                                                                                        |                                                                                           
GATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGG  >  NC_000913/1691363‑1691542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCG  >  NC_000913/1691358‑1691544
                                                                                             |                                                                                             
CAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGAC                                                                                               <  SRR4302540.4134714/94‑1 (MQ=60)
    AGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCCGCACCAGCGGTGCCGACGCCA                                                                                           >  SRR4302540.7254417/1‑94 (MQ=60)
     aaaAACCGCAGGTCCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGCCCACCCCCGGCACCAGCGGTGCCGACGCCAC                                                                                          <  SRR4302540.7466081/91‑1 (MQ=60)
     GATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCAC                                                                                          <  SRR4302540.351369/94‑1 (MQ=60)
        AACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGT                                                                                       <  SRR4302540.6639580/94‑1 (MQ=60)
        AACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGT                                                                                       >  SRR4302540.7768817/1‑94 (MQ=60)
          CCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTAC                                                                                     <  SRR4302540.6080624/94‑1 (MQ=60)
            GCAGGTTCCCAGGAAAGGCCAAATCAGGAAGGTATTCTTTTTACCGATACTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCA                                                                                   <  SRR4302540.3322135/94‑1 (MQ=60)
            GCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGCCCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCA                                                                                   <  SRR4302540.815587/94‑1 (MQ=60)
            GCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCA                                                                                   >  SRR4302540.9947898/1‑94 (MQ=60)
            GCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCA                                                                                   >  SRR4302540.9555504/1‑94 (MQ=60)
               GGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACC                                                                                >  SRR4302540.3301256/1‑94 (MQ=60)
                GTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCA                                                                               <  SRR4302540.276949/94‑1 (MQ=60)
                 TTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAG                                                                              >  SRR4302540.10078221/1‑94 (MQ=60)
                   CCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGT                                                                            >  SRR4302540.254853/1‑94 (MQ=60)
                   CCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCAACCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGT                                                                            >  SRR4302540.3628250/1‑94 (MQ=60)
                      AGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTT                                                                         <  SRR4302540.627142/94‑1 (MQ=60)
                      AGCAAAGCGCCAATCAGGAAGCTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTT                                                                         >  SRR4302540.1176052/1‑94 (MQ=60)
                       GCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTG                                                                        >  SRR4302540.317176/1‑94 (MQ=60)
                        CAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGC                                                                       >  SRR4302540.825205/1‑94 (MQ=60)
                        CAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGGTTTGC                                                                       >  SRR4302540.1993347/1‑94 (MQ=60)
                        CAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGCTTTGC                                                                       >  SRR4302540.7728495/1‑94 (MQ=60)
                         AAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCA                                                                      >  SRR4302540.5074979/1‑94 (MQ=60)
                              GCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGT                                                                 >  SRR4302540.7662605/1‑94 (MQ=60)
                              GCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCCGCCCCAGCGGGGCCGTCGCCACGGTACCAACCAGGTTTTCCACCAGT                                                                 >  SRR4302540.7662604/1‑94 (MQ=60)
                                CAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGGCGACGCCACAGTACCAACCAGGCTTTGCACCAGTAC                                                               >  SRR4302540.968880/1‑94 (MQ=60)
                                 AATCAGGAAGGTATTCTTTTTCCCGATCCCCGCGCCCACCCCCGCCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACC                                                              <  SRR4302540.2073351/94‑1 (MQ=60)
                                  ATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCA                                                             >  SRR4302540.1198567/1‑94 (MQ=60)
                                  ATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCA                                                             <  SRR4302540.837070/94‑1 (MQ=60)
                                  ATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCA                                                             >  SRR4302540.8663489/1‑94 (MQ=60)
                                  ATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCA                                                             >  SRR4302540.8663490/1‑94 (MQ=60)
                                   TCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAG                                                            >  SRR4302540.1188219/1‑94 (MQ=60)
                                   TCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAG                                                            <  SRR4302540.647879/94‑1 (MQ=60)
                                      GGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGCACCAGCAC                                                         >  SRR4302540.4183742/1‑94 (MQ=60)
                                      AGAAGTTATTCTTGTTACCGATCCGCGCGACCACCCCGGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCAC                                                         <  SRR4302540.780807/94‑1 (MQ=60)
                                       gaggtttttctttttcccgaccctcgcgcccacccccgcccccggggtTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACA                                                        <  SRR4302540.8963855/46‑1 (MQ=60)
                                        AAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCCCCACCCGTGCCGACGCCCCAGTACCGACCAGGTTTTGCAACACTACCACCACAG                                                       >  SRR4302540.3920622/1‑94 (MQ=53)
                                         AGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGT                                                      <  SRR4302540.6366376/94‑1 (MQ=60)
                                         AGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGT                                                      >  SRR4302540.9672471/1‑94 (MQ=60)
                                         AGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTCGCACCAGTACCAGCACAGT                                                      >  SRR4302540.4194162/1‑94 (MQ=60)
                                         AGGTATTCTCTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGT                                                      <  SRR4302540.1527766/94‑1 (MQ=60)
                                          GGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTG                                                     >  SRR4302540.7248618/1‑94 (MQ=60)
                                          GGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGGGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTG                                                     >  SRR4302540.8305409/1‑94 (MQ=60)
                                            TATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGaagcgctag                                                   >  SRR4302540.4568430/1‑85 (MQ=60)
                                            TATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGATTTGCAACAGTACCAGCACAGTGAC                                                   >  SRR4302540.4723461/1‑94 (MQ=60)
                                             ATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAAC                                                  >  SRR4302540.4679500/1‑94 (MQ=60)
                                             ATTCTTTTTACCGACCCTCGCGACCACCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAAC                                                  <  SRR4302540.7955781/94‑1 (MQ=60)
                                              TTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACA                                                 <  SRR4302540.1313316/94‑1 (MQ=60)
                                              TTCTTTTTACCGATCCTCGCGACCACCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACA                                                 <  SRR4302540.504591/94‑1 (MQ=60)
                                               TCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAG                                                <  SRR4302540.1219889/94‑1 (MQ=60)
                                               TCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAG                                                <  SRR4302540.4974683/94‑1 (MQ=60)
                                                 TTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCC                                              >  SRR4302540.1057019/1‑94 (MQ=60)
                                                    TTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAAACAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGG                                           >  SRR4302540.3764347/1‑94 (MQ=60)
                                                     TACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGT                                          >  SRR4302540.5750232/1‑94 (MQ=60)
                                                     TACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGT                                          >  SRR4302540.8135371/1‑94 (MQ=60)
                                                      ACCGATCCTCGCGACCATCCCCGGCACCAGCGGGGCCGACGCCACAGTACCAACCAGGGTTTGCACCAGTACCAGCACAGTGAACAGCCCGGGA                                         >  SRR4302540.9140294/1‑94 (MQ=60)
                                                       CCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTAT                                        <  SRR4302540.1106608/94‑1 (MQ=60)
                                                          ATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAT                                     >  SRR4302540.3819223/1‑94 (MQ=60)
                                                          ATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAT                                     >  SRR4302540.7864340/1‑94 (MQ=60)
                                                           ccctcgcccCCATCCCCGGCACCAGCGGTCCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATT                                    <  SRR4302540.611750/85‑1 (MQ=60)
                                                           TCCTCGCGCCCATCCCCGGCCCCAGCGGTCCCGACGCCCCAGTACCAGCCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATT                                    <  SRR4302540.5009292/94‑1 (MQ=60)
                                                            CCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTT                                   >  SRR4302540.6199706/1‑94 (MQ=60)
                                                             CTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTA                                  >  SRR4302540.8658817/1‑94 (MQ=60)
                                                             CTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTA                                  >  SRR4302540.8658818/1‑94 (MQ=60)
                                                              ccgccacccccccgGGCACCGGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAA                                 <  SRR4302540.2116541/80‑1 (MQ=60)
                                                              TCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAA                                 >  SRR4302540.2548349/1‑94 (MQ=60)
                                                                ccccccccccccgcccCCAGCGGCGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACA                               <  SRR4302540.7275133/78‑1 (MQ=60)
                                                                GCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACA                               <  SRR4302540.3226995/94‑1 (MQ=60)
                                                                  GACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACA                             >  SRR4302540.869308/1‑94 (MQ=60)
                                                                    CCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATA                           <  SRR4302540.4505703/94‑1 (MQ=60)
                                                                    CCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATA                           >  SRR4302540.6399388/1‑94 (MQ=60)
                                                                    CCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATA                           >  SRR4302540.6399386/1‑94 (MQ=60)
                                                                     cccCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAG                          <  SRR4302540.1095600/91‑1 (MQ=60)
                                                                     CATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGACCAGCCCGGTATCATTTAACACATAG                          >  SRR4302540.417678/1‑94 (MQ=60)
                                                                     CATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAG                          >  SRR4302540.36582/1‑94 (MQ=60)
                                                                      accccggccccgAGCGGTCCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGC                         <  SRR4302540.161630/82‑1 (MQ=60)
                                                                      ATCCCCGGCACCAGTGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGC                         >  SRR4302540.7111950/1‑94 (MQ=60)
                                                                      ATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGC                         >  SRR4302540.8660180/1‑94 (MQ=60)
                                                                         CCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAGCAGCCCGGTATCATTTAACACATAGCGCA                      >  SRR4302540.224830/1‑94 (MQ=60)
                                                                           CGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACG                    <  SRR4302540.2613259/94‑1 (MQ=60)
                                                                            GGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGT                   >  SRR4302540.7639519/1‑94 (MQ=60)
                                                                            GGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGT                   <  SRR4302540.8869805/94‑1 (MQ=60)
                                                                             GCACCGGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTA                  <  SRR4302540.5748457/94‑1 (MQ=60)
                                                                               ACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGA                <  SRR4302540.304088/94‑1 (MQ=60)
                                                                               ACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGA                >  SRR4302540.3952534/1‑94 (MQ=60)
                                                                               ACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGA                >  SRR4302540.6585363/1‑94 (MQ=60)
                                                                                CCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAA               <  SRR4302540.8243095/94‑1 (MQ=60)
                                                                                 CAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAAC              <  SRR4302540.3286733/94‑1 (MQ=60)
                                                                                 CAGCGGTGCCGACGCCACAGTACCAACCAGGTTTCGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAAC              <  SRR4302540.1023808/94‑1 (MQ=60)
                                                                                  AGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACA             >  SRR4302540.5116104/1‑94 (MQ=60)
                                                                                  AGCGGGGCCGACGCCACGGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACA             <  SRR4302540.7808254/94‑1 (MQ=60)
                                                                                   GCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAA            >  SRR4302540.9257696/1‑94 (MQ=60)
                                                                                   GCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAA            >  SRR4302540.2078457/1‑94 (MQ=60)
                                                                                    CGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAAC           <  SRR4302540.5798245/94‑1 (MQ=60)
                                                                                    CGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAAC           <  SRR4302540.1591866/94‑1 (MQ=60)
                                                                                     GGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACG          <  SRR4302540.7963271/94‑1 (MQ=60)
                                                                                      GTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGA         <  SRR4302540.6234673/94‑1 (MQ=60)
                                                                                        GCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACG       <  SRR4302540.360277/94‑1 (MQ=60)
                                                                                          CGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAG     <  SRR4302540.534819/94‑1 (MQ=60)
                                                                                          CGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAG     <  SRR4302540.887127/94‑1 (MQ=60)
                                                                                          CGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAG     <  SRR4302540.642347/94‑1 (MQ=60)
                                                                                           GATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGG    >  SRR4302540.3837138/1‑94 (MQ=60)
                                                                                           GACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGG    <  SRR4302540.989729/94‑1 (MQ=60)
                                                                                             CGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCG  >  SRR4302540.5150210/1‑94 (MQ=60)
                                                                                             |                                                                                             
CAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCG  >  NC_000913/1691358‑1691544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑