Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,691,451	T→C	A279A (GCA→GCG)	uidB ←	glucuronide permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,691,451	0	T	C	100.0%	696.7 / NA	219	A279A (GCA→GCG)	uidB	glucuronide permease
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (116/103); total (116/103)

Only 100 of 227 total aligned reads displayed.

AGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGA  >  NC_000913/1691362‑1691540

   tAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCcaca                                                                                     <  1:4102592/93‑1 (MQ=255)

    aaCCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAg                                                                                    >  1:5062627/1‑93 (MQ=255)

     aCCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGt                                                                                   >  1:3048633/1‑93 (MQ=255)

     aCCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGt                                                                                   >  2:5914728/1‑93 (MQ=255)

         cAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTacca                                                                               >  1:2673512/1‑93 (MQ=255)

         cAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTacca                                                                               <  1:4971542/93‑1 (MQ=255)

         cAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTacca                                                                               >  1:2366992/1‑93 (MQ=255)

          aGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTaccaa                                                                              >  2:3833199/1‑93 (MQ=255)

          aGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTaccaa                                                                              >  1:3089310/1‑93 (MQ=255)

            gtccccAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTaccaacc                                                                            <  1:2957872/90‑1 (MQ=255)

            gTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTaccaacc                                                                            <  2:5081735/93‑1 (MQ=255)

               cccAGAAAAGCGCAAATCAGGAGGTTATTCTTTTTCCGGATCCTCGCGCCCACCCCGGGCACAAGCGGTGCCGACGCCACAGTACCAACCAgg                                                                         <  2:5713567/93‑1 (MQ=255)

                 cAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGtt                                                                       >  1:779578/1‑93 (MQ=255)

                  aGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGttt                                                                      >  1:1134953/1‑93 (MQ=255)

                   gCAAAGCGCCAATCAGGAAGGTATTGTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGtttt                                                                     >  2:305250/1‑93 (MQ=255)

                     aaaGCGCCAATCAGGAAGTTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGc                                                                   <  2:490490/93‑1 (MQ=255)

                     aaaGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGc                                                                   >  2:1435449/1‑93 (MQ=255)

                     aaaGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGc                                                                   >  2:2652211/1‑93 (MQ=255)

                      aaGCGCCAATTAGAAATGTATTCTTGTTCCCGTTCCTCGCGACCACCCCCGGCCCCAGCGGTGCGGACGCCACAGTACCAACCAGGTTTTGCa                                                                  <  1:1537034/93‑1 (MQ=255)

                      aaGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCa                                                                  >  1:5104591/1‑93 (MQ=255)

                       acccccAATAAGGAAGGTTTTTTTTTTCCCCACCCCCGCGCCCCCCCCCGCCACCGGGGGGGCCGACGCCACAGTACCAACCAGGTTTTGCAc                                                                 <  2:1259972/89‑1 (MQ=255)

                       aGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCAc                                                                 <  1:1768632/93‑1 (MQ=255)

                           ccAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGt                                                             >  2:1533482/1‑93 (MQ=255)

                            cAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTa                                                            >  1:3524206/1‑93 (MQ=255)

                              aTCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTAcc                                                          <  2:3791504/93‑1 (MQ=255)

                                 aGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGc                                                       >  1:4799790/1‑93 (MQ=255)

                                  ggAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTTCACCAGAACCAGca                                                      >  2:69059/1‑93 (MQ=255)

                                  ggAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGca                                                      <  1:3511528/93‑1 (MQ=255)

                                  ggAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGca                                                      >  2:2371636/1‑93 (MQ=255)

                                  ggAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGca                                                      >  1:2466180/1‑93 (MQ=255)

                                   gAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGcac                                                     >  1:847122/1‑93 (MQ=255)

                                   gAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGcac                                                     <  2:880850/93‑1 (MQ=255)

                                     aGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAg                                                   >  2:3390047/1‑93 (MQ=255)

                                      ggTATTTTTTTTCCCGATCCTCGCGACCACCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGt                                                  <  1:4654617/93‑1 (MQ=255)

                                       gTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGta                                                 >  1:200561/1‑92 (MQ=255)

                                        tATTTTTTTCACCGCCCCCCGCGCCCACCCCCGGCCCCAGCGGTGCGGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGa                                                <  2:1199292/93‑1 (MQ=255)

                                        tATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGa                                                >  1:5841330/1‑93 (MQ=255)

                                         aTTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGCGCCGACGCCACAGTACCAACCAGGGTTTGCACCAGTACCAGCACAGTCaa                                               >  2:5382649/1‑93 (MQ=255)

                                          ttCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGGTTTGCACCAGTACCAGCACAGTGAAc                                              >  2:4377371/1‑93 (MQ=255)

                                           tttttttGCCGGTCCTCGCCACCATCCCCGGCACCAGGGGTGCGGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACa                                             <  1:3713609/91‑1 (MQ=255)

                                           tttttttCCCGATCCTCGCGACCACCCCCGGCCCCAGGGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACa                                             <  1:5567274/91‑1 (MQ=255)

                                           tCTTTTGCCCGCTCCCCGCGACCACCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACa                                             <  2:5479658/93‑1 (MQ=255)

                                            cTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAg                                            >  2:2636106/1‑93 (MQ=255)

                                             tttttACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGc                                           <  1:3981161/93‑1 (MQ=255)

                                             tttttACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAAGGAACAGc                                           >  1:106564/1‑93 (MQ=255)

                                              ttttACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCCACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGcc                                          >  1:4295477/1‑93 (MQ=255)

                                               tttACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGccc                                         <  2:512084/93‑1 (MQ=255)

                                                 tACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCgg                                       >  2:1004811/1‑93 (MQ=255)

                                                 tACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCgg                                       >  1:4156466/1‑93 (MQ=255)

                                                   ccGATCCTCGCGACCACCCCCGGCACCAGCGGTCCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTa                                     <  2:3616397/93‑1 (MQ=255)

                                                    cGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTAt                                    >  1:1814662/1‑93 (MQ=255)

                                                     gATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATc                                   >  1:709860/1‑93 (MQ=255)

                                                     gATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATc                                   >  1:3441133/1‑93 (MQ=255)

                                                     gATCCTCGCGACCATCCCCGGCACCAACGGTGACGACGCCACAGTACCAACCAGGATTTGGACCAGTACCAGCACAGTGAACAGCCCGGTATc                                   >  2:5971312/1‑93 (MQ=255)

                                                      aTCCTCGCGACCATCCCCGGCACCAGCGGTTCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCa                                  <  1:2845811/93‑1 (MQ=255)

                                                      aTCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCa                                  >  2:87536/1‑93 (MQ=255)

                                                      aTCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCa                                  <  1:2519879/93‑1 (MQ=255)

                                                       tCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAt                                 >  1:4455853/1‑93 (MQ=255)

                                                       tCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAt                                 >  1:1623695/1‑93 (MQ=255)

                                                       tCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAt                                 >  2:791493/1‑93 (MQ=255)

                                                        ccTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGGACCAACCAGGTTTTGCACCAGTACCAGCACCGTGAACAGCCCGGGATCAtt                                >  2:2266445/1‑93 (MQ=255)

                                                         cTCGCGACCATCTTCGGCTTCTGCGGGTCCTACTCCATAGTACCAACCAGGCTTTGCACCAGTACCAGCACAGTTAACATCTCTGTAACAtat                               >  1:6296969/1‑91 (MQ=255)

                                                          tCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTa                              >  2:4887336/1‑93 (MQ=255)

                                                             cgACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAaca                           <  1:5867741/93‑1 (MQ=255)

                                                             cgACCACCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAaca                           <  1:4935540/93‑1 (MQ=255)

                                                              gACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAacac                          >  2:1697516/1‑93 (MQ=255)

                                                               aCCATCCCCGGCACCAGCGTTGCCAACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAAcaca                         <  2:6065308/93‑1 (MQ=255)

                                                               aCCATCCCCGGCACCAGCGGTGCTGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAAcaca                         <  1:2618724/93‑1 (MQ=255)

                                                               aCCATCCCCGGCACCAGCGGTGCCGACGCCTCAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAAcaca                         <  2:702661/93‑1 (MQ=255)

                                                               aCCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAAcaca                         >  2:916433/1‑93 (MQ=255)

                                                               aCCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAAcaca                         >  1:1911327/1‑93 (MQ=255)

                                                                  acccccGGCCCCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAg                      <  2:243919/91‑1 (MQ=255)

                                                                  aTCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAg                      >  1:3403663/1‑93 (MQ=255)

                                                                  aTCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAg                      <  1:3932680/93‑1 (MQ=255)

                                                                  aTCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAg                      <  1:1009932/93‑1 (MQ=255)

                                                                   tCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAgc                     <  1:3172604/93‑1 (MQ=255)

                                                                   tCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAgc                     >  2:3943643/1‑93 (MQ=255)

                                                                   tCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAgc                     >  2:2930970/1‑93 (MQ=255)

                                                                   tCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAgc                     <  2:2561502/93‑1 (MQ=255)

                                                                   cccccGGCCCCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAgc                     <  2:4442192/92‑1 (MQ=255)

                                                                     cccGGCACCAGCGTCGCCCACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAgcgc                   <  2:3356410/93‑1 (MQ=255)

                                                                     cccGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAgcgc                   >  2:4508353/1‑93 (MQ=255)

                                                                     cccGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAgcgc                   <  2:4835271/93‑1 (MQ=255)

                                                                     cccGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAgcgc                   >  1:3579828/1‑93 (MQ=255)

                                                                      ccGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCa                  <  2:3840996/93‑1 (MQ=255)

                                                                      ccGCCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCa                  <  1:1394503/93‑1 (MQ=255)

                                                                       cGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCAc                 >  2:3532809/1‑93 (MQ=255)

                                                                        ggCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACg                <  1:6181496/93‑1 (MQ=255)

                                                                          cACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTa              <  2:1624386/93‑1 (MQ=255)

                                                                            ccAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGa            >  2:2216084/1‑93 (MQ=255)

                                                                              aGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaac          >  2:5202229/1‑93 (MQ=255)

                                                                              aGCGGGCCCGACGCCACTGTACCAACCAGGCGTTGCACCAGGACCAGCCCAGTGACCCGCCCAGTATCATTTAACACATAGTGCCCGTAGgac          >  2:6220535/1‑93 (MQ=255)

                                                                               gCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaaca         >  2:2035388/1‑93 (MQ=255)

                                                                                cGGTCCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaacaa        <  2:31987/93‑1 (MQ=255)

                                                                                 ggTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaacaac       <  2:5062628/93‑1 (MQ=255)

                                                                                 ggTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaacaac       <  1:6276668/93‑1 (MQ=255)

                                                                                   tGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAacga     <  2:5555196/93‑1 (MQ=255)

                                                                                   tGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAacga     <  2:2891716/93‑1 (MQ=255)

                                                                                      cGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAAcgacga  <  1:5271435/93‑1 (MQ=255)

                                                                                      cGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAAcgacga  <  2:4868656/93‑1 (MQ=255)

AGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGA  >  NC_000913/1691362‑1691540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCG  >  NC_000913/1691362‑1691544

AGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCCCCAGCGGTGCCGACGCCA                                                                                           <  SRR4302533.4941207/94‑1 (MQ=60)

   TAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAG                                                                                        <  SRR4302533.4127554/94‑1 (MQ=60)

   TAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAG                                                                                        >  SRR4302533.5093218/1‑94 (MQ=60)

   TAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAG                                                                                        >  SRR4302533.5093217/1‑94 (MQ=60)

   TAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAG                                                                                        <  SRR4302533.3077527/94‑1 (MQ=60)

    AACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGT                                                                                       <  SRR4302533.1077858/94‑1 (MQ=60)

    AACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGT                                                                                       >  SRR4302533.3067297/1‑94 (MQ=60)

    AACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGT                                                                                       <  SRR4302533.1825269/94‑1 (MQ=60)

     ACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTA                                                                                      <  SRR4302533.703006/94‑1 (MQ=60)

     ACCGCAGGTCCCCAGAAAAGCGCAAATCAGGAAGGTATTCTTTTTACCGTCCCTCGCGACCATCCCCGGCACCAGGGGTGCCGACGCCACAGTA                                                                                      <  SRR4302533.2732620/94‑1 (MQ=60)

        GCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCA                                                                                   >  SRR4302533.2381635/1‑94 (MQ=60)

        GCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCA                                                                                   >  SRR4302533.2689911/1‑94 (MQ=60)

         CAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGGACCAGCGGTGCCGACGCCACAGTACCAA                                                                                  >  SRR4302533.4865882/1‑94 (MQ=60)

         CAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAA                                                                                  <  SRR4302533.5001685/94‑1 (MQ=60)

         CAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAA                                                                                  >  SRR4302533.3108300/1‑94 (MQ=60)

            GTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCA                                                                               <  SRR4302533.5220686/94‑1 (MQ=60)

            GTCCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCA                                                                               <  SRR4302533.2975950/94‑1 (MQ=60)

              TCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGG                                                                             <  SRR4302533.809510/94‑1 (MQ=60)

                CCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTT                                                                           >  SRR4302533.784170/1‑94 (MQ=60)

                 CAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTT                                                                          >  SRR4302533.1141702/1‑94 (MQ=60)

                  AGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTT                                                                         <  SRR4302533.4253396/94‑1 (MQ=60)

                  AGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTT                                                                         <  SRR4302533.5185144/94‑1 (MQ=60)

                     AAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCA                                                                      >  SRR4302533.5135427/1‑94 (MQ=60)

                      AAGCGCCAATTAGAAATGTATTCTTGTTCCCGTTCCTCGCGACCACCCCCGGCCCCAGCGGTGCGGACGCCACAGTACCAACCAGGTTTTGCAC                                                                     <  SRR4302533.1546268/94‑1 (MQ=42)

                       AGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACC                                                                    <  SRR4302533.1779401/94‑1 (MQ=60)

                           CCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTA                                                                >  SRR4302533.3545927/1‑94 (MQ=60)

                            CAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTAC                                                               >  SRR4302533.153056/1‑94 (MQ=60)

                             aaccgggaggtTATTCTTTTAACCGATCCTCGCGCCCATCCCCGACACCCGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACC                                                              <  SRR4302533.5672523/83‑1 (MQ=60)

                             AATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACC                                                              >  SRR4302533.4305953/1‑94 (MQ=60)

                              ATCAGGAAGATATTCTTTTCCCCGATCCTCGCGACCCTCCCCGGCACCAGCGTTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCA                                                             <  SRR4302533.3504461/94‑1 (MQ=60)

                               TCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAG                                                            <  SRR4302533.2540493/94‑1 (MQ=60)

                               TCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAG                                                            <  SRR4302533.6248501/94‑1 (MQ=60)

                                CAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGC                                                           >  SRR4302533.4828864/1‑94 (MQ=60)

                                 AGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCA                                                          >  SRR4302533.2481393/1‑94 (MQ=60)

                                 AGAAAGGTTTTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCA                                                          <  SRR4302533.5899831/94‑1 (MQ=60)

                                  GGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCAC                                                         <  SRR4302533.3533173/94‑1 (MQ=60)

                                  GGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCAC                                                         >  SRR4302533.852080/1‑94 (MQ=60)

                                    AAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAG                                                       <  SRR4302533.5895753/94‑1 (MQ=60)

                                    AAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAG                                                       >  SRR4302533.2510719/1‑94 (MQ=60)

                                     AGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGT                                                      >  SRR4302533.923934/1‑94 (MQ=60)

                                      GGTATTTTTTTTCCCGATCCTCGCGACCACCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTG                                                     <  SRR4302533.4682820/94‑1 (MQ=60)

                                      GGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTG                                                     >  SRR4302533.1533376/1‑94 (MQ=60)

                                      GGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTA                                                     >  SRR4302533.201832/1‑94 (MQ=60)

                                       GTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGA                                                    >  SRR4302533.5876648/1‑94 (MQ=60)

                                        TATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAA                                                   >  SRR4302533.2007174/1‑94 (MQ=60)

                                         ATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAAC                                                  >  SRR4302533.1826465/1‑94 (MQ=60)

                                          TTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGGTTTGCACCAGTACCAGCACAGTGAACA                                                 >  SRR4302533.122229/1‑94 (MQ=60)

                                           TTTTTTTGCCGGTCCTCGCCACCATCCCCGGCACCAGGGGTGCGGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAG                                                <  SRR4302533.3736410/94‑1 (MQ=60)

                                           TTTTTTTCCCGATCCTCGCGACCACCCCCGGCCCCAGGGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAG                                                <  SRR4302533.5600926/94‑1 (MQ=60)

                                            CTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAAGGAACAGC                                               >  SRR4302533.107232/1‑94 (MQ=60)

                                             TTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCCACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCC                                              >  SRR4302533.4321309/1‑94 (MQ=60)

                                             TTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCC                                              <  SRR4302533.4005400/94‑1 (MQ=60)

                                             TTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCC                                              >  SRR4302533.722252/1‑94 (MQ=60)

                                               TTTACCGATCCTCGCGCCCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCG                                            <  SRR4302533.3653392/94‑1 (MQ=60)

                                               TTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCG                                            <  SRR4302533.3887594/94‑1 (MQ=60)

                                               TTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCG                                            >  SRR4302533.313287/1‑94 (MQ=60)

                                               TTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCG                                            >  SRR4302533.4582467/1‑94 (MQ=60)

                                                TTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGG                                           >  SRR4302533.4181647/1‑94 (MQ=60)

                                                  cccgacccccgcgcccacCCCCGGCACCAGGGGTCCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTA                                         <  SRR4302533.5394928/76‑1 (MQ=60)

                                                  CCCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTA                                         <  SRR4302533.2888548/94‑1 (MQ=60)

                                                  ACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACCCCACAGTAACAACCAGGTTTTGCACCAGTACCAGCACAGcgaaaaggccggga                                         >  SRR4302533.5486973/1‑80 (MQ=60)

                                                   CCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTAT                                        >  SRR4302533.2521942/1‑94 (MQ=60)

                                                   CCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTAT                                        >  SRR4302533.1825740/1‑94 (MQ=60)

                                                    CGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATC                                       >  SRR4302533.3462381/1‑94 (MQ=60)

                                                    CGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATC                                       >  SRR4302533.714052/1‑94 (MQ=60)

                                                     GATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCA                                      >  SRR4302533.3965390/1‑94 (MQ=60)

                                                     GATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCA                                      <  SRR4302533.2673153/94‑1 (MQ=60)

                                                      ATCCTCGCGACCATCCCCGGCACCAGCGGTTCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAT                                     <  SRR4302533.2863325/94‑1 (MQ=60)

                                                      ATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAT                                     >  SRR4302533.4482797/1‑94 (MQ=60)

                                                      ATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAT                                     <  SRR4302533.2535406/94‑1 (MQ=60)

                                                      ATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAT                                     >  SRR4302533.1633530/1‑94 (MQ=60)

                                                        CCTCGCGACCATCCCCGGCACCAGCGGGGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTT                                   >  SRR4302533.4565140/1‑94 (MQ=60)

                                                         CTCGCGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGGACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCCGTATCATTTA                                  >  SRR4302533.68025/1‑94 (MQ=60)

                                                            CCGACCACCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACA                               <  SRR4302533.2980687/94‑1 (MQ=60)

                                                             CGACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACAC                              <  SRR4302533.5903206/94‑1 (MQ=60)

                                                             CGACCACCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACAC                              <  SRR4302533.4965427/94‑1 (MQ=60)

                                                             CGACCACCCCCGCCACCAGCGGCCCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACAC                              <  SRR4302533.3843700/94‑1 (MQ=60)

                                                              GACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACA                             >  SRR4302533.1922990/1‑94 (MQ=60)

                                                              GACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACA                             >  SRR4302533.5745822/1‑94 (MQ=60)

                                                              GACCATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACA                             <  SRR4302533.4997546/94‑1 (MQ=60)

                                                               ACCATCCCCGGCACCAGCGGTGCTGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACAT                            <  SRR4302533.2634801/94‑1 (MQ=60)

                                                                 CATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAG                          <  SRR4302533.4126537/94‑1 (MQ=60)

                                                                 CATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAG                          >  SRR4302533.2480507/1‑94 (MQ=60)

                                                                 CATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAG                          >  SRR4302533.3536459/1‑94 (MQ=60)

                                                                 CATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAG                          >  SRR4302533.3424680/1‑94 (MQ=60)

                                                                  ATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGC                         <  SRR4302533.3956687/94‑1 (MQ=60)

                                                                  ATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGC                         <  SRR4302533.610430/94‑1 (MQ=60)

                                                                  ATCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGC                         <  SRR4302533.1015838/94‑1 (MQ=60)

                                                                   TCCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCG                        <  SRR4302533.3192134/94‑1 (MQ=60)

                                                                   CCCCCGCCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCG                        <  SRR4302533.3535784/94‑1 (MQ=60)

                                                                    CCCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGC                       >  SRR4302533.3601886/1‑94 (MQ=60)

                                                                     CCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCA                      >  SRR4302533.3146068/1‑94 (MQ=60)

                                                                     CCCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCA                      >  SRR4302533.3333669/1‑94 (MQ=60)

                                                                      CCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCAC                     <  SRR4302533.633385/94‑1 (MQ=60)

                                                                      CCGGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCAC                     <  SRR4302533.5037741/94‑1 (MQ=60)

                                                                      CCGCCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCAC                     <  SRR4302533.1402906/94‑1 (MQ=60)

                                                                       CGGCACCAGCGGTGCCGACGCCACGGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACG                    >  SRR4302533.3436633/1‑94 (MQ=60)

                                                                       CGGCACCAGCGGGGCCGACGCCACAGTACCAAACAAGTTTTGCACCAGTAACAGCACAAGGAACAAGCCGGTATCATTTAACACATAGCGCACG                    >  SRR4302533.3320948/1‑94 (MQ=53)

                                                                        GGCACCAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGT                   <  SRR4302533.6218617/94‑1 (MQ=60)

                                                                         GCACCAGCGGTGCCGACGCCACAGTACCAACCAGGCTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTA                  >  SRR4302533.4792314/1‑94 (MQ=60)

                                                                             CAGCGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAAC              <  SRR4302533.4171046/94‑1 (MQ=60)

                                                                                CGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAAC           <  SRR4302533.1567527/94‑1 (MQ=60)

                                                                                CGGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAAC           >  SRR4302533.2743641/1‑94 (MQ=60)

                                                                                 GGTGCCGACGCCACAGTCCCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACG          <  SRR4302533.358398/94‑1 (MQ=60)

                                                                                 GGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACG          <  SRR4302533.6314148/94‑1 (MQ=60)

                                                                                 GGTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACG          >  SRR4302533.1065177/1‑94 (MQ=60)

                                                                                  GTGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGA         <  SRR4302533.1655113/94‑1 (MQ=60)

                                                                                   TGCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGAC        >  SRR4302533.2799218/1‑94 (MQ=60)

                                                                                    GCCGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACG       <  SRR4302533.2176139/94‑1 (MQ=60)

                                                                                      CGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAG     >  SRR4302533.281000/1‑94 (MQ=60)

                                                                                      CGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAG     <  SRR4302533.4378983/94‑1 (MQ=60)

                                                                                      CGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAG     <  SRR4302533.5303050/94‑1 (MQ=60)

                                                                                      CGACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAG     <  SRR4302533.1882739/94‑1 (MQ=60)

                                                                                       GACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGG    <  SRR4302533.5635164/94‑1 (MQ=60)

                                                                                       GACGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGG    <  SRR4302533.4112030/94‑1 (MQ=60)

                                                                                         CGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCG  <  SRR4302533.2826280/94‑1 (MQ=60)

                                                                                         CGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCG  <  SRR4302533.2628436/94‑1 (MQ=60)

                                                                                         CGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCG  <  SRR4302533.5189342/94‑1 (MQ=60)

AGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCG  >  NC_000913/1691362‑1691544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`