Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,890,225 C→T R345R (CGG→CGA)  b1808 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,890,2250CT100.0% 658.9 / NA 178R345R (CGG→CGA) b1808hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (88/90);  total (88/90)

Only 100 of 185 total aligned reads displayed.

GAGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGCCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTT  >  NC_000913/1890135‑1890313
                                                                                          |                                                                                        
gAGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCg                                                                                        >  2:4507382/1‑93 (MQ=255)
 aGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCgt                                                                                       <  2:6047202/93‑1 (MQ=255)
  gcggcgTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCgtg                                                                                      >  2:189903/1‑93 (MQ=255)
   cggcgTGAGAGCGAGAGTAAAAGGGCGGCAAGTTCAGTGGTACCAGTAGGTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCgtgt                                                                                     <  1:5531910/93‑1 (MQ=255)
     gcgTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGc                                                                                   <  1:2512997/93‑1 (MQ=255)
       gTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGa                                                                                 >  1:2013809/1‑93 (MQ=255)
        tGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGAt                                                                                >  2:2238747/1‑93 (MQ=255)
        ggagaGCCAGAGTAAAATGGCGCGAAGTGCATGCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGAt                                                                                <  2:5346222/92‑1 (MQ=255)
         gagaGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATa                                                                               >  1:5109278/1‑93 (MQ=255)
         gagaGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATa                                                                               <  2:5205542/93‑1 (MQ=255)
         gagaGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATa                                                                               <  1:4033481/93‑1 (MQ=255)
          agagCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATAc                                                                              >  2:6268108/1‑93 (MQ=255)
          agagCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATAc                                                                              <  2:2379591/93‑1 (MQ=255)
           gagCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACa                                                                             <  1:1638077/93‑1 (MQ=255)
           gagCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACa                                                                             >  2:4654868/1‑93 (MQ=255)
            agCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACaa                                                                            <  1:717719/93‑1 (MQ=255)
             gCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAAc                                                                           <  1:1167878/93‑1 (MQ=255)
              ccAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACg                                                                          <  1:4478400/93‑1 (MQ=255)
               cAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGt                                                                         <  1:528773/93‑1 (MQ=255)
                agagTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTg                                                                        >  2:850639/1‑93 (MQ=255)
                agagTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTg                                                                        <  2:1642496/93‑1 (MQ=255)
                agagTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTg                                                                        >  2:4477984/1‑93 (MQ=255)
                  agTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGgc                                                                      >  2:6301548/1‑93 (MQ=255)
                  agTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGgc                                                                      >  1:2257902/1‑93 (MQ=255)
                   gTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGgcg                                                                     >  2:1061143/1‑93 (MQ=255)
                    tAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGgcgc                                                                    >  2:310639/1‑93 (MQ=255)
                      aaaTGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGgcgcgc                                                                  >  1:3326131/1‑93 (MQ=255)
                      aaaTGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGgcgcgc                                                                  <  1:1882392/93‑1 (MQ=255)
                      aaaTGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGgcgcgc                                                                  >  2:2198904/1‑93 (MQ=255)
                      aaaTGGCGCGAAGTGCATTCGTACCAGTAGCTATAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGgcgcgc                                                                  >  1:1291581/1‑93 (MQ=255)
                       aaTGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCt                                                                 <  1:368848/93‑1 (MQ=255)
                            cgcgAAGTGCATTCGTACCAGTAGCTGTAGCTCGGCTGATTGAGCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAAt                                                            <  1:3221314/93‑1 (MQ=255)
                            cgcgAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGCTCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAAt                                                            >  1:4433641/1‑93 (MQ=255)
                             gcgAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATg                                                           >  2:3006990/1‑93 (MQ=255)
                              cgAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGc                                                          >  1:3855714/1‑93 (MQ=255)
                               gAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATggc                                                         >  2:2266267/1‑91 (MQ=255)
                                aaGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCg                                                        >  2:3944124/1‑93 (MQ=255)
                                 aGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGc                                                       >  2:2561578/1‑93 (MQ=255)
                                   tGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCACGCTCAAATGCCGCAt                                                     >  2:2783333/1‑93 (MQ=255)
                                    gCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATc                                                    <  2:5895920/93‑1 (MQ=255)
                                     cATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATGCAACGTGGCGCGCTCAAATGCCGCATcc                                                   <  2:6093004/93‑1 (MQ=255)
                                      aTTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCa                                                  <  2:904681/93‑1 (MQ=255)
                                       ttCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAg                                                 >  1:4165865/1‑93 (MQ=255)
                                       ttCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAg                                                 >  2:5929971/1‑93 (MQ=255)
                                          gTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCaa                                              <  1:5998374/93‑1 (MQ=255)
                                          gTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCaa                                              <  1:1725468/93‑1 (MQ=255)
                                           tACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAg                                             <  1:5121082/93‑1 (MQ=255)
                                           tACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAg                                             >  1:50961/1‑93 (MQ=255)
                                            aCCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAgg                                            <  2:3738263/93‑1 (MQ=255)
                                            aCCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAgg                                            >  1:4797236/1‑93 (MQ=255)
                                             ccAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGc                                           >  1:5393073/1‑93 (MQ=255)
                                               aGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCgg                                         >  2:4158598/1‑93 (MQ=255)
                                                gTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGa                                        <  1:1349533/93‑1 (MQ=255)
                                                 tAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGaa                                       <  1:269720/93‑1 (MQ=255)
                                                  aGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGTATCCAGCAAGGCGGAAc                                      >  1:3452633/1‑93 (MQ=255)
                                                   gCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACg                                     >  2:2147468/1‑93 (MQ=255)
                                                   gCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGACGCATCCAGCAAGGCGGAACg                                     >  2:1701245/1‑93 (MQ=255)
                                                     tGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGcc                                   >  1:5821479/1‑93 (MQ=255)
                                                      gTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGccc                                  >  1:361679/1‑93 (MQ=255)
                                                       tAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGcccc                                 <  1:4389419/93‑1 (MQ=255)
                                                       tAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGcccc                                 >  1:3833708/1‑93 (MQ=255)
                                                          cccGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGt                              <  2:2634606/93‑1 (MQ=255)
                                                            cGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGa                            >  2:2604939/1‑93 (MQ=255)
                                                                tgattgatCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGt                        >  2:2240194/1‑93 (MQ=255)
                                                                  attgatCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGttt                      >  1:3923627/1‑93 (MQ=255)
                                                                  attgatCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGttt                      <  1:4739329/93‑1 (MQ=255)
                                                                   ttgatCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTc                     <  1:4336633/93‑1 (MQ=255)
                                                                   ttgatCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTc                     >  2:4964386/1‑93 (MQ=255)
                                                                   ttgatCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTc                     <  1:238503/93‑1 (MQ=255)
                                                                    tgatCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCg                    >  2:5263912/1‑93 (MQ=255)
                                                                     gatCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGc                   >  2:3075011/1‑93 (MQ=255)
                                                                       tctcTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCa                 <  2:5470769/93‑1 (MQ=255)
                                                                          ctTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGt              >  1:2990851/1‑93 (MQ=255)
                                                                           ttttAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCGACGTc             >  1:406142/1‑93 (MQ=255)
                                                                           ttttAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTc             >  1:4302105/1‑93 (MQ=255)
                                                                           ttttAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTc             <  1:2901701/93‑1 (MQ=255)
                                                                             ttAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCAt           <  1:4628634/93‑1 (MQ=255)
                                                                              tAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCTGCAAGGCGGATCGCCCCAGTGACAGTTTCGCCACGTCATa          <  1:1726300/93‑1 (MQ=255)
                                                                              tAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATa          <  2:2046606/93‑1 (MQ=255)
                                                                               aGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATaa         <  1:6082927/93‑1 (MQ=255)
                                                                               aGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATaa         >  1:2670364/1‑93 (MQ=255)
                                                                                gCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAAc        >  2:3172218/1‑93 (MQ=255)
                                                                                gCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAAc        <  1:567206/93‑1 (MQ=255)
                                                                                gCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAAc        >  2:2336040/1‑93 (MQ=255)
                                                                                gCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAAc        <  2:4044134/93‑1 (MQ=255)
                                                                                gCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAAc        >  2:1171329/1‑93 (MQ=255)
                                                                                 ccGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACa       >  1:1172916/1‑93 (MQ=255)
                                                                                  cGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACaa      >  2:3854912/1‑93 (MQ=255)
                                                                                  cGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACaa      <  1:1539222/93‑1 (MQ=255)
                                                                                   gCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCTGAACGCCCCAGTGACAGTTTCGCCACGTCATAACaaa     <  2:4901301/93‑1 (MQ=255)
                                                                                   gCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACaaa     <  2:1566955/93‑1 (MQ=255)
                                                                                   gCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACaaa     <  2:1757765/93‑1 (MQ=255)
                                                                                   gCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACaaa     >  2:6033743/1‑93 (MQ=255)
                                                                                   gCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACaaa     >  1:4523059/1‑93 (MQ=255)
                                                                                   gCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACaaa     >  2:3323987/1‑93 (MQ=255)
                                                                                    cTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAg    >  2:5785481/1‑93 (MQ=255)
                                                                                    cTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAg    <  1:3540005/93‑1 (MQ=255)
                                                                                     ttCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGTCACGTCATAACAAAGt   >  1:777893/1‑93 (MQ=255)
                                                                                     ttCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGt   >  2:5585375/1‑93 (MQ=255)
                                                                                      tCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGtt  <  2:2427017/93‑1 (MQ=255)
                                                                                          |                                                                                        
GAGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGCCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTT  >  NC_000913/1890135‑1890313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGAGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGCCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAG  >  NC_000913/1890133‑1890317
                                                                                            |                                                                                            
CTGAGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTC                                                                                             <  SRR4302533.3005424/94‑1 (MQ=60)
 TGAGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCG                                                                                            <  SRR4302533.4059582/94‑1 (MQ=60)
 TGAGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCG                                                                                            <  SRR4302533.6162202/94‑1 (MQ=60)
  GAGCGGCGTGAGAGCCAGAGTAAAGTGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGT                                                                                           <  SRR4302533.5151551/94‑1 (MQ=60)
   AGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTG                                                                                          <  SRR4302533.5112116/94‑1 (MQ=60)
     CGGCGTGAGAGCGAGAGTAAAAGGGCGGCAAGTTCAGTGGTACCAGTAGGTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTG                                                                                        <  SRR4302533.5565310/94‑1 (MQ=53)
      GGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGC                                                                                       <  SRR4302533.2475400/94‑1 (MQ=60)
       GCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCG                                                                                      <  SRR4302533.2528481/94‑1 (MQ=60)
        CGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGA                                                                                     >  SRR4302533.2026216/1‑94 (MQ=60)
        CGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGA                                                                                     >  SRR4302533.1883681/1‑94 (MQ=60)
         GTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGAT                                                                                    <  SRR4302533.3666742/94‑1 (MQ=60)
          TGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATA                                                                                   >  SRR4302533.5140152/1‑94 (MQ=60)
           GAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATAC                                                                                  <  SRR4302533.4058032/94‑1 (MQ=60)
            AGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACA                                                                                 <  SRR4302533.3771649/94‑1 (MQ=60)
            AGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACA                                                                                 <  SRR4302533.2980587/94‑1 (MQ=60)
             GAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAA                                                                                <  SRR4302533.1647994/94‑1 (MQ=60)
              AGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAAC                                                                               <  SRR4302533.721956/94‑1 (MQ=60)
               GCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACG                                                                              <  SRR4302533.1174884/94‑1 (MQ=60)
               GCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACG                                                                              >  SRR4302533.4766148/1‑94 (MQ=60)
                CCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGT                                                                             <  SRR4302533.4505496/94‑1 (MQ=60)
                CCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGT                                                                             <  SRR4302533.5533545/94‑1 (MQ=60)
                 CAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTG                                                                            >  SRR4302533.1213438/1‑94 (MQ=60)
                 CAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTG                                                                            <  SRR4302533.531918/94‑1 (MQ=60)
                   GAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGC                                                                          >  SRR4302533.2271874/1‑94 (MQ=60)
                    AGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCG                                                                         <  SRR4302533.3978703/94‑1 (MQ=60)
                     GTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGC                                                                        <  SRR4302533.4651391/94‑1 (MQ=60)
                       AAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGC                                                                      >  SRR4302533.3346682/1‑94 (MQ=60)
                       AAAATGGCGCGAAGTGCATTCGTACCAGTAGCTATAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGC                                                                      >  SRR4302533.1299362/1‑94 (MQ=60)
                        AAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCT                                                                     <  SRR4302533.1893853/94‑1 (MQ=60)
                         AATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTC                                                                    <  SRR4302533.371075/94‑1 (MQ=60)
                           TGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAA                                                                  <  SRR4302533.4382352/94‑1 (MQ=60)
                             GCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGCTCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAAT                                                                >  SRR4302533.4460446/1‑94 (MQ=60)
                             GCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAAT                                                                <  SRR4302533.4173718/94‑1 (MQ=60)
                              CGCGAAGTGCATTCGTACCAGTAGCTGTAGCTCGGCTGATTGAGCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATG                                                               <  SRR4302533.3241178/94‑1 (MQ=60)
                               GCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGC                                                              >  SRR4302533.3879257/1‑94 (MQ=60)
                                CGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCC                                                             <  SRR4302533.5716643/94‑1 (MQ=60)
                                   AGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCA                                                          <  SRR4302533.627983/94‑1 (MQ=60)
                                    GTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCAT                                                         <  SRR4302533.4273832/94‑1 (MQ=60)
                                        ATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAG                                                     >  SRR4302533.4191108/1‑94 (MQ=60)
                                           CGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAA                                                  <  SRR4302533.845169/94‑1 (MQ=60)
                                            GTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAG                                                 <  SRR4302533.1735938/94‑1 (MQ=60)
                                            GTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAG                                                 <  SRR4302533.6034703/94‑1 (MQ=60)
                                            GTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAG                                                 >  SRR4302533.51308/1‑94 (MQ=60)
                                             TACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGG                                                <  SRR4302533.5152022/94‑1 (MQ=60)
                                             TACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGG                                                >  SRR4302533.4826295/1‑94 (MQ=60)
                                              ACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGC                                               >  SRR4302533.5425475/1‑94 (MQ=60)
                                                CAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTAGCGCGCTCAAATGCCGCATACAGCAAGGCGG                                             >  SRR4302533.3865294/1‑94 (MQ=60)
                                                 AGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGA                                            >  SRR4302533.2616475/1‑94 (MQ=60)
                                                  GTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAA                                           <  SRR4302533.1357651/94‑1 (MQ=60)
                                                   TAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGTATCCAGCAAGGCGGAAC                                          >  SRR4302533.3473950/1‑94 (MQ=60)
                                                   TAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAAC                                          <  SRR4302533.271358/94‑1 (MQ=60)
                                                    AGCTGTAGCTCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACG                                         <  SRR4302533.1984559/94‑1 (MQ=60)
                                                     GCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGC                                        >  SRR4302533.2089491/1‑94 (MQ=60)
                                                     GCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGC                                        >  SRR4302533.3244328/1‑94 (MQ=60)
                                                      CTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCC                                       >  SRR4302533.5856642/1‑94 (MQ=60)
                                                       TGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCC                                      >  SRR4302533.363877/1‑94 (MQ=60)
                                                        GTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCC                                     >  SRR4302533.3857139/1‑94 (MQ=60)
                                                         TAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCA                                    <  SRR4302533.4415920/94‑1 (MQ=60)
                                                         TAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCA                                    <  SRR4302533.5367390/94‑1 (MQ=60)
                                                          AGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAATGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAG                                   >  SRR4302533.1393033/1‑94 (MQ=60)
                                                          AGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAG                                   >  SRR4302533.3879705/1‑94 (MQ=60)
                                                            CCCGGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTG                                 <  SRR4302533.2855890/94‑1 (MQ=60)
                                                              CGGCTTATTGATCCCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGAC                               <  SRR4302533.355799/94‑1 (MQ=60)
                                                               GGCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACA                              <  SRR4302533.5129787/94‑1 (MQ=60)
                                                                GCTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAG                             >  SRR4302533.3063808/1‑94 (MQ=60)
                                                                 CTGATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGT                            >  SRR4302533.445354/1‑94 (MQ=60)
                                                                   GATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTT                          >  SRR4302533.3947583/1‑94 (MQ=60)
                                                                    ATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTC                         <  SRR4302533.1346109/94‑1 (MQ=60)
                                                                    ATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTC                         <  SRR4302533.4477886/94‑1 (MQ=60)
                                                                    ATTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTC                         <  SRR4302533.4768064/94‑1 (MQ=60)
                                                                     TTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCG                        <  SRR4302533.239979/94‑1 (MQ=60)
                                                                     TTGATCTCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCG                        <  SRR4302533.4362767/94‑1 (MQ=60)
                                                                           TCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGT                  >  SRR4302533.3009143/1‑94 (MQ=60)
                                                                           TCTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGT                  >  SRR4302533.1583032/1‑94 (MQ=60)
                                                                            CTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCGACGTC                 >  SRR4302533.408583/1‑94 (MQ=60)
                                                                            CTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTC                 <  SRR4302533.4772451/94‑1 (MQ=60)
                                                                            CTTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTC                 >  SRR4302533.4327985/1‑94 (MQ=60)
                                                                             TTTTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCA                <  SRR4302533.2919526/94‑1 (MQ=60)
                                                                               TTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATA              <  SRR4302533.4656686/94‑1 (MQ=60)
                                                                               TTAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATA              <  SRR4302533.4328685/94‑1 (MQ=60)
                                                                                TAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCTGCAAGGCGGATCGCCCCAGTGACAGTTTCGCCACGTCATAA             <  SRR4302533.1736774/94‑1 (MQ=60)
                                                                                TAGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAA             >  SRR4302533.2686743/1‑94 (MQ=60)
                                                                                 AGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAAC            >  SRR4302533.5621412/1‑94 (MQ=60)
                                                                                 AGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAAC            <  SRR4302533.3470784/94‑1 (MQ=60)
                                                                                 AGCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAAC            <  SRR4302533.6119707/94‑1 (MQ=60)
                                                                                  GCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACA           <  SRR4302533.743230/94‑1 (MQ=60)
                                                                                  GCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACA           <  SRR4302533.570563/94‑1 (MQ=60)
                                                                                  GCCGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACA           >  SRR4302533.1179956/1‑94 (MQ=60)
                                                                                    CGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAA         <  SRR4302533.1648313/94‑1 (MQ=60)
                                                                                    CGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAA         >  SRR4302533.4550439/1‑94 (MQ=60)
                                                                                    CGCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAA         <  SRR4302533.1548488/94‑1 (MQ=60)
                                                                                     GCTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAG        <  SRR4302533.5955950/94‑1 (MQ=60)
                                                                                      CTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGTCACGTCATAACAAAGT       >  SRR4302533.782471/1‑94 (MQ=60)
                                                                                      CTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGT       <  SRR4302533.2271963/94‑1 (MQ=60)
                                                                                      CTTCAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGT       <  SRR4302533.3561817/94‑1 (MQ=60)
                                                                                         CAGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCC    >  SRR4302533.4700404/1‑94 (MQ=60)
                                                                                          AGTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCA   >  SRR4302533.5937581/1‑94 (MQ=60)
                                                                                           GTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAG  <  SRR4302533.5533169/94‑1 (MQ=60)
                                                                                           GTCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAG  >  SRR4302533.2383562/1‑94 (MQ=60)
                                                                                            |                                                                                            
CTGAGCGGCGTGAGAGCCAGAGTAAAATGGCGCGAAGTGCATTCGTACCAGTAGCTGTAGCCCGGCTGATTGATCTCTTTTAGCCGCTTCAGCCGTGTGCGATACAACGTGGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAG  >  NC_000913/1890133‑1890317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑