Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,920,902	T→C	Q31R (CAG→CGG)	b1838 ←	protein phosphatase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,920,902	0	T	C	99.6%	860.1 / NA	269	Q31R (CAG→CGG)	b1838	protein phosphatase 1
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (131/137); total (132/137)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

CTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTC  >  NC_000913/1920812‑1920991

cTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGc                                                                                         >  1:4300430/1‑93 (MQ=255)

cTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGc                                                                                         >  2:2983551/1‑93 (MQ=255)

  ttGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTc                                                                                       >  2:3304406/1‑93 (MQ=255)

   tGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCa                                                                                      <  1:586583/93‑1 (MQ=255)

       gCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGAc                                                                                  >  1:120736/1‑93 (MQ=255)

        cccACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACa                                                                                 >  2:5200775/1‑93 (MQ=255)

         ccACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACaa                                                                                >  2:2932553/1‑93 (MQ=255)

          cACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAAc                                                                               <  1:5768534/93‑1 (MQ=255)

            cgatcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCg                                                                             <  1:216595/93‑1 (MQ=255)

             gatcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCgt                                                                            <  2:4777204/93‑1 (MQ=255)

             gatcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCgt                                                                            <  2:5746166/93‑1 (MQ=255)

              atcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCgtg                                                                           >  2:168726/1‑93 (MQ=255)

              atcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCgtg                                                                           >  1:787809/1‑93 (MQ=255)

              atcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCgtg                                                                           >  1:2062940/1‑93 (MQ=255)

               tcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCgtgt                                                                          <  2:5374568/93‑1 (MQ=255)

               tcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCgtgt                                                                          >  1:3712210/1‑93 (MQ=255)

                cgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGta                                                                         <  2:2104352/93‑1 (MQ=255)

                cgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGta                                                                         <  2:4909276/93‑1 (MQ=255)

                  atAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGtata                                                                       <  2:3327560/93‑1 (MQ=255)

                  atAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGtata                                                                       >  1:4908093/1‑93 (MQ=255)

                   tAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTatat                                                                      >  2:3442542/1‑93 (MQ=255)

                   tAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTatat                                                                      >  1:5400157/1‑93 (MQ=255)

                   tAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTatat                                                                      >  1:2554374/1‑93 (MQ=255)

                    aaCGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCACCCGCTCAAGACAACCGTGTATATc                                                                     <  1:6237808/93‑1 (MQ=255)

                    aaCGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATc                                                                     >  1:5001886/1‑93 (MQ=255)

                          tcCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGa                                                               <  2:3105637/93‑1 (MQ=255)

                          tcCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGa                                                               >  1:3005020/1‑93 (MQ=255)

                          tcCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGa                                                               >  1:6030904/1‑93 (MQ=255)

                            ccACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGaaa                                                             >  2:1167974/1‑93 (MQ=255)

                            ccACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGaaa                                                             >  2:2230438/1‑93 (MQ=255)

                            ccACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGaaa                                                             >  1:4541306/1‑93 (MQ=255)

                             cACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAg                                                            <  1:327076/93‑1 (MQ=255)

                               cTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGACAGca                                                          >  2:5625444/1‑92 (MQ=255)

                               cTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGcc                                                          >  2:4242067/1‑93 (MQ=255)

                               cTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGcc                                                          <  1:5964098/93‑1 (MQ=255)

                                tGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCa                                                         >  2:2454856/1‑93 (MQ=255)

                                tGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCa                                                         <  1:3667146/93‑1 (MQ=255)

                                 gcgaTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAg                                                        >  2:3295886/3‑93 (MQ=255)

                                  agaTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGa                                                       >  1:266163/1‑93 (MQ=255)

                                  agaTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGa                                                       <  2:815301/93‑1 (MQ=255)

                                   gaTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGat                                                      <  2:956937/93‑1 (MQ=255)

                                    aTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGata                                                     <  2:3840020/93‑1 (MQ=255)

                                     tAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGatat                                                    >  2:2862797/1‑93 (MQ=255)

                                     tAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGatat                                                    >  1:5554076/1‑93 (MQ=255)

                                     tAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGatat                                                    <  2:3587999/93‑1 (MQ=255)

                                     tAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGatat                                                    >  1:5435783/1‑93 (MQ=255)

                                     tAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGatat                                                    <  2:5789607/93‑1 (MQ=255)

                                        gTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTc                                                 <  1:820699/93‑1 (MQ=255)

                                         tAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCg                                                <  1:2215111/93‑1 (MQ=255)

                                          aaaTCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGc                                               <  1:1694662/93‑1 (MQ=255)

                                            aTCTCGCCACGTAACAAAACGACAATGCCATTATTCGCGGCGCAACCGCCCAAGACATCCGTGTATACCGCCATAAAGCCAGATATGTCGCCa                                             <  2:6145891/93‑1 (MQ=255)

                                             tctcGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCAt                                            >  1:3571840/1‑93 (MQ=255)

                                              ctcGCCACGGATCAAAACGACAATGCCATAATCTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCAtt                                           <  1:5451072/93‑1 (MQ=255)

                                               tcGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATtg                                          >  1:1858861/1‑93 (MQ=255)

                                               tcGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATtg                                          <  1:1059198/93‑1 (MQ=255)

                                                cGCCACGGATCAAAACGACAATGGCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATtga                                         <  1:6123609/93‑1 (MQ=255)

                                                cGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTGAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATtga                                         <  2:1451952/93‑1 (MQ=255)

                                                cGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATtga                                         <  1:5908343/93‑1 (MQ=255)

                                                    aCGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGAc                                     >  1:2341245/1‑93 (MQ=255)

                                                    aCGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGAc                                     <  2:4604296/93‑1 (MQ=255)

                                                    aCGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGAc                                     <  1:4798118/93‑1 (MQ=255)

                                                     cGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGAcc                                    <  1:1615128/93‑1 (MQ=255)

                                                         tCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCa                                >  1:5711284/1‑93 (MQ=255)

                                                         tCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCa                                >  2:5576491/1‑93 (MQ=255)

                                                           aaaaCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAt                              >  2:2263825/1‑93 (MQ=255)

                                                           aaaaCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAt                              >  2:900444/1‑93 (MQ=255)

                                                           aaaaCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAt                              >  2:791834/1‑93 (MQ=255)

                                                           aaaaCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAt                              <  1:3803288/93‑1 (MQ=255)

                                                           aaaaCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAt                              >  1:3634514/1‑93 (MQ=255)

                                                           aaaaCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAt                              <  2:1956340/93‑1 (MQ=255)

                                                           aaaaCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAt                              <  2:5393537/93‑1 (MQ=255)

                                                             aaCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATtc                            >  2:2365152/1‑93 (MQ=255)

                                                             aaCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATtc                            <  1:1477928/93‑1 (MQ=255)

                                                                gACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTctct                         <  1:418098/93‑1 (MQ=255)

                                                                gACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTctct                         >  2:2185391/1‑93 (MQ=255)

                                                                 aCAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTg                        >  2:599763/1‑93 (MQ=255)

                                                                  cAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGa                       <  2:4989488/93‑1 (MQ=255)

                                                                   aaTGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGAt                      <  1:5093909/93‑1 (MQ=255)

                                                                    aTGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATa                     <  2:2505213/93‑1 (MQ=255)

                                                                      gCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATaaa                   <  1:642237/93‑1 (MQ=255)

                                                                      gCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATaaa                   >  1:5317056/1‑93 (MQ=255)

                                                                       ccATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAAc                  >  1:2797664/1‑93 (MQ=255)

                                                                          tAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACggg               <  2:6272491/93‑1 (MQ=255)

                                                                          tAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACggg               <  1:2492658/93‑1 (MQ=255)

                                                                          tAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACggg               <  1:3147560/93‑1 (MQ=255)

                                                                            aTTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGgcg             <  1:3898389/93‑1 (MQ=255)

                                                                             tttGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGgcgc            <  2:1929706/93‑1 (MQ=255)

                                                                             tttGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGgcgc            >  2:3733993/1‑93 (MQ=255)

                                                                              ttGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCa           <  1:2932670/93‑1 (MQ=255)

                                                                              ttGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCa           <  2:4362833/93‑1 (MQ=255)

                                                                              ttGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCa           <  2:2736913/93‑1 (MQ=255)

                                                                                 cggcgCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGc        <  2:1003774/93‑1 (MQ=255)

                                                                                 cggcgCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGc        <  1:4779591/93‑1 (MQ=255)

                                                                                   gcgcAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTg      <  2:1074035/93‑1 (MQ=255)

                                                                                   gcgcAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTg      >  2:4139164/1‑93 (MQ=255)

                                                                                    cgcAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGt     >  2:5546360/1‑93 (MQ=255)

                                                                                    cgcAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGt     <  1:3323261/93‑1 (MQ=255)

                                                                                    cgcAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGt     <  1:2714972/93‑1 (MQ=255)

                                                                                     gcAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGtt    <  2:5916100/93‑1 (MQ=255)

                                                                                      cAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGttt   >  2:4056181/1‑93 (MQ=255)

CTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTC  >  NC_000913/1920812‑1920991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAACTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCATCA  >  NC_000913/1920809‑1920995

AAACTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACC                                                                                               <  SRR4302533.1661032/94‑1 (MQ=60)

 AACTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCG                                                                                              >  SRR4302533.248759/1‑94 (MQ=60)

  ACTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGC                                                                                             >  SRR4302533.4326293/1‑94 (MQ=60)

      TGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAA                                                                                         <  SRR4302533.590046/94‑1 (MQ=60)

      TGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAA                                                                                         <  SRR4302533.2800704/94‑1 (MQ=60)

        CGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGA                                                                                       >  SRR4302533.5370645/1‑94 (MQ=60)

         GGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGAC                                                                                      >  SRR4302533.121500/1‑94 (MQ=60)

          GCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACA                                                                                     <  SRR4302533.2072845/94‑1 (MQ=60)

           CCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAA                                                                                    >  SRR4302533.962225/1‑94 (MQ=60)

             CACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACC                                                                                  <  SRR4302533.5803357/94‑1 (MQ=60)

             CACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACC                                                                                  >  SRR4302533.4964764/1‑94 (MQ=60)

             CACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACC                                                                                  <  SRR4302533.3609348/94‑1 (MQ=60)

             CACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACC                                                                                  >  SRR4302533.378969/1‑94 (MQ=60)

              ACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCG                                                                                 >  SRR4302533.480873/1‑94 (MQ=60)

               CGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGT                                                                                <  SRR4302533.217955/94‑1 (MQ=60)

               CGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGT                                                                                <  SRR4302533.5720080/94‑1 (MQ=60)

                GATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTG                                                                               <  SRR4302533.4843521/94‑1 (MQ=60)

                GATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTG                                                                               >  SRR4302533.792435/1‑94 (MQ=60)

                GATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTG                                                                               >  SRR4302533.2075659/1‑94 (MQ=60)

                GATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTG                                                                               >  SRR4302533.1856309/1‑94 (MQ=60)

                 ATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGT                                                                              >  SRR4302533.3735006/1‑94 (MQ=60)

                    GATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATA                                                                           >  SRR4302533.4605344/1‑94 (MQ=60)

                    GATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATA                                                                           >  SRR4302533.4937830/1‑94 (MQ=60)

                     ATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATAT                                                                          >  SRR4302533.2570089/1‑94 (MQ=60)

                     ATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATAT                                                                          >  SRR4302533.5432606/1‑94 (MQ=60)

                      TAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATC                                                                         >  SRR4302533.5032207/1‑94 (MQ=60)

                       AACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCACCCGCTCAAGACAACCGTGTATATCG                                                                        <  SRR4302533.6275159/94‑1 (MQ=60)

                          GTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCA                                                                     >  SRR4302533.4413556/1‑94 (MQ=60)

                           TCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAG                                                                    >  SRR4302533.594453/1‑94 (MQ=60)

                           TCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAG                                                                    <  SRR4302533.2041705/94‑1 (MQ=60)

                           TCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAG                                                                    <  SRR4302533.1658553/94‑1 (MQ=60)

                            CTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGA                                                                   >  SRR4302533.6067453/1‑94 (MQ=60)

                            CTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGA                                                                   >  SRR4302533.3023401/1‑94 (MQ=60)

                             CCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCACCCGCTCAAGACAACCGTGTATATCGCCAGAA                                                                  <  SRR4302533.5932826/94‑1 (MQ=60)

                              CCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAA                                                                 >  SRR4302533.4568805/1‑94 (MQ=60)

                               CCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAG                                                                <  SRR4302533.3912489/94‑1 (MQ=60)

                               CCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAG                                                                >  SRR4302533.614300/1‑94 (MQ=60)

                                CACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGC                                                               <  SRR4302533.329066/94‑1 (MQ=60)

                                CACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGC                                                               >  SRR4302533.5895217/1‑94 (MQ=60)

                                 ACTGAGATAAGTAAATCTTGCCTCGGATCAATACGACAATGCCATAATTTTCGGCGCAATTGCTCAAGACAACCGTGTATATCGCCTGTAAGCC                                                              >  SRR4302533.6341477/1‑94 (MQ=60)

                                 ACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCC                                                              >  SRR4302533.578044/1‑94 (MQ=60)

                                 ACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCC                                                              >  SRR4302533.5505141/1‑94 (MQ=60)

                                 ACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCC                                                              >  SRR4302533.5481872/1‑94 (MQ=60)

                                  CTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCA                                                             <  SRR4302533.6000183/94‑1 (MQ=60)

                                  CTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCA                                                             >  SRR4302533.4094128/1‑94 (MQ=60)

                                   TGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAG                                                            >  SRR4302533.63693/1‑94 (MQ=60)

                                   TGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAG                                                            <  SRR4302533.3689686/94‑1 (MQ=60)

                                    GAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGA                                                           <  SRR4302533.3159364/94‑1 (MQ=60)

                                    GAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGA                                                           >  SRR4302533.267782/1‑94 (MQ=60)

                                      GATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATA                                                         >  SRR4302533.374368/1‑94 (MQ=60)

                                       ATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATAT                                                        >  SRR4302533.5468518/1‑94 (MQ=60)

                                       ATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATAT                                                        >  SRR4302533.5587617/1‑94 (MQ=60)

                                       ATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATAT                                                        <  SRR4302533.1508461/94‑1 (MQ=60)

                                         AAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGT                                                      >  SRR4302533.2792053/1‑94 (MQ=60)

                                         AAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGT                                                      <  SRR4302533.678616/94‑1 (MQ=60)

                                          AGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTC                                                     <  SRR4302533.6256182/94‑1 (MQ=60)

                                          AGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTC                                                     <  SRR4302533.816181/94‑1 (MQ=60)

                                           GTNNNTNTCGCCACGGATCAAANCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCG                                                    <  SRR4302533.2575119/94‑1 (MQ=60)

                                           GTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGTCAACCCTGTATATGGCCACAAAGCAAGATATGTCG                                                    >  SRR4302533.2050521/1‑94 (MQ=60)

                                           GTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCG                                                    <  SRR4302533.825512/94‑1 (MQ=60)

                                            TAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGC                                                   >  SRR4302533.2960205/1‑94 (MQ=60)

                                            TAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGC                                                   <  SRR4302533.2228783/94‑1 (MQ=60)

                                             AAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCC                                                  <  SRR4302533.1704929/94‑1 (MQ=60)

                                               ATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCAT                                                >  SRR4302533.598552/1‑94 (MQ=60)

                                               ATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCAT                                                >  SRR4302533.252788/1‑94 (MQ=60)

                                               ATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCAT                                                >  SRR4302533.3593851/1‑94 (MQ=60)

                                               ATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCAT                                                >  SRR4302533.3306747/1‑94 (MQ=60)

                                                 CTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTG                                              >  SRR4302533.1870196/1‑94 (MQ=60)

                                                 CTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTG                                              >  SRR4302533.3128278/1‑94 (MQ=60)

                                                 CTCGCCACGGATCAAAACGACAATGCCATAATCTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTG                                              <  SRR4302533.5483911/94‑1 (MQ=60)

                                                  TCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGA                                             >  SRR4302533.5452430/1‑94 (MQ=60)

                                                  TCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGA                                             <  SRR4302533.560441/94‑1 (MQ=60)

                                                  TCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGA                                             >  SRR4302533.5452429/1‑94 (MQ=60)

                                                  TCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGA                                             <  SRR4302533.5930759/94‑1 (MQ=60)

                                                  TCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGA                                             <  SRR4302533.1065427/94‑1 (MQ=60)

                                                   CGCCACGGATCAAAACGACAATGGCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGAT                                            <  SRR4302533.6160524/94‑1 (MQ=60)

                                                   CGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGAT                                            <  SRR4302533.5477085/94‑1 (MQ=60)

                                                   CGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGAT                                            <  SRR4302533.5944033/94‑1 (MQ=60)

                                                   CGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGAT                                            <  SRR4302533.6017091/94‑1 (MQ=60)

                                                    GCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATG                                           >  SRR4302533.498241/1‑94 (MQ=60)

                                                     CCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGA                                          <  SRR4302533.3239280/94‑1 (MQ=60)

                                                     CCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGA                                          <  SRR4302533.362568/94‑1 (MQ=60)

                                                     CCACGGATCAAAACGACAACGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGC                                          >  SRR4302533.5058286/1‑94 (MQ=60)

                                                      CACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGAC                                         >  SRR4302533.656098/1‑94 (MQ=60)

                                                      CACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGAC                                         >  SRR4302533.2355738/1‑94 (MQ=60)

                                                       ACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACC                                        <  SRR4302533.2678781/94‑1 (MQ=60)

                                                       ACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACC                                        >  SRR4302533.2437042/1‑94 (MQ=60)

                                                       ACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACC                                        <  SRR4302533.4827181/94‑1 (MQ=60)

                                                        CGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCC                                       >  SRR4302533.2651702/1‑94 (MQ=60)

                                                        CGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCC                                       <  SRR4302533.1624921/94‑1 (MQ=60)

                                                        CGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCGCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCC                                       <  SRR4302533.1322745/94‑1 (MQ=60)

                                                          GATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGC                                     >  SRR4302533.3686306/1‑94 (MQ=60)

                                                           ATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCA                                    >  SRR4302533.2235586/1‑94 (MQ=60)

                                                           ATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCA                                    >  SRR4302533.5745783/1‑94 (MQ=60)

                                                             CAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAT                                  <  SRR4302533.2078343/94‑1 (MQ=60)

                                                             CAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAT                                  >  SRR4302533.3656868/1‑94 (MQ=60)

                                                              AAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATT                                 <  SRR4302533.3826559/94‑1 (MQ=60)

                                                                AACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCT                               <  SRR4302533.1486857/94‑1 (MQ=60)

                                                                 ACGACAATGCCATAATTTGCGCCGCAACCCCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTTATGACCCGCAATTCTC                              <  SRR4302533.1590096/94‑1 (MQ=60)

                                                                  CGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCT                             <  SRR4302533.4329221/94‑1 (MQ=60)

                                                                  CGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCT                             <  SRR4302533.5775176/94‑1 (MQ=60)

                                                                   GACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTG                            <  SRR4302533.420604/94‑1 (MQ=60)

                                                                   GACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTG                            <  SRR4302533.341810/94‑1 (MQ=60)

                                                                    ACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGA                           >  SRR4302533.2447677/1‑94 (MQ=60)

                                                                      AATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATA                         <  SRR4302533.3857157/94‑1 (MQ=60)

                                                                      AATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATA                         <  SRR4302533.5124680/94‑1 (MQ=60)

                                                                       ATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAA                        >  SRR4302533.2255335/1‑94 (MQ=60)

                                                                        TGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAA                       >  SRR4302533.5348921/1‑94 (MQ=60)

                                                                        TGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAA                       >  SRR4302533.2151321/1‑94 (MQ=60)

                                                                        TGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAA                       >  SRR4302533.2227827/1‑94 (MQ=60)

                                                                        TGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAA                       >  SRR4302533.2899784/1‑94 (MQ=60)

                                                                         GCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAAC                      <  SRR4302533.646012/94‑1 (MQ=60)

                                                                         GCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAAC                      >  SRR4302533.2814870/1‑94 (MQ=60)

                                                                           CATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGG                    <  SRR4302533.4749255/94‑1 (MQ=60)

                                                                             TAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGC                  <  SRR4302533.3166912/94‑1 (MQ=60)

                                                                             TAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGC                  <  SRR4302533.1796506/94‑1 (MQ=60)

                                                                             TAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGC                  <  SRR4302533.504987/94‑1 (MQ=60)

                                                                             TAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGC                  <  SRR4302533.2508012/94‑1 (MQ=60)

                                                                             TAATCTGCCGCGCAACCGCTCAAGACAACCGTGTATGTCGCCAGAAAGCCAGATATGTCGCCATTGATGACCGGCAATTCTCTGATAAACGGGC                  <  SRR4302533.1439099/94‑1 (MQ=60)

                                                                               ATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGC                >  SRR4302533.2978126/1‑94 (MQ=60)

                                                                               ATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGC                >  SRR4302533.626388/1‑94 (MQ=60)

                                                                               ATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGC                >  SRR4302533.2547512/1‑94 (MQ=60)

                                                                               ATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGC                >  SRR4302533.371450/1‑94 (MQ=60)

                                                                               ATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGC                <  SRR4302533.3922173/94‑1 (MQ=60)

                                                                                 TTGCGGCGCGACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAG              <  SRR4302533.6041599/94‑1 (MQ=60)

                                                                                 TTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAG              <  SRR4302533.2950605/94‑1 (MQ=60)

                                                                                 TTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAG              <  SRR4302533.1068102/94‑1 (MQ=60)

                                                                                  TGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGG             <  SRR4302533.3243839/94‑1 (MQ=60)

                                                                                  TGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGGCGCCATGGATGACCCGCAATTCTCTGATAAACGGGCGCAGG             >  SRR4302533.909921/1‑94 (MQ=60)

                                                                                   GCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGC            <  SRR4302533.4872008/94‑1 (MQ=60)

                                                                                    CGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCT           <  SRR4302533.3538378/94‑1 (MQ=60)

                                                                                    CGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCT           <  SRR4302533.4808545/94‑1 (MQ=60)

                                                                                    CGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCT           <  SRR4302533.4296275/94‑1 (MQ=60)

                                                                                       CGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTT        <  SRR4302533.5419236/94‑1 (MQ=60)

                                                                                       CGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTT        <  SRR4302533.3343786/94‑1 (MQ=60)

                                                                                       CGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTT        <  SRR4302533.2731641/94‑1 (MQ=60)

                                                                                        GCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTT       <  SRR4302533.4254316/94‑1 (MQ=60)

                                                                                         CAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTC      >  SRR4302533.3676602/1‑94 (MQ=60)

                                                                                         CAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTC      >  SRR4302533.2686974/1‑94 (MQ=60)

                                                                                          AACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCA     >  SRR4302533.545465/1‑94 (MQ=60)

                                                                                          AACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCA     <  SRR4302533.5267258/94‑1 (MQ=60)

                                                                                           ACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCAT    >  SRR4302533.3463261/1‑94 (MQ=60)

                                                                                            CCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCATC   >  SRR4302533.5482093/1‑94 (MQ=60)

                                                                                             CGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCATCA  >  SRR4302533.5252758/1‑94 (MQ=60)

                                                                                             CGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCATCA  >  SRR4302533.324895/1‑94 (MQ=60)

AAACTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCATCA  >  NC_000913/1920809‑1920995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`