Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,930,097	(A)_7→6	intergenic (+14/+42)	purT → / ← eda	phosphoribosylglycinamide formyltransferase 2/2‑keto‑3‑deoxygluconate 6‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,930,091	0	A	.	99.5%	829.4 / ‑3.2	181	intergenic (+8/+48)	purT/eda	phosphoribosylglycinamide formyltransferase 2/2‑keto‑3‑deoxygluconate 6‑phosphate aldolase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (80/100); total (80/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

GGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAA  >  NC_000913/1929999‑1930182

     cACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaaa                                                                                        >  2:5606364/1‑93 (MQ=255)

      aCTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaaac                                                                                       <  1:5146214/93‑2 (MQ=255)

        tGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaaacgc                                                                                     >  1:5212558/1‑90 (MQ=255)

          gCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTa                                                                                  <  1:1065963/93‑1 (MQ=255)

           cTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTAc                                                                                 <  1:5673345/93‑1 (MQ=255)

           cTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTAc                                                                                 <  1:2639957/93‑1 (MQ=255)

           cTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTAc                                                                                 <  1:1870159/93‑1 (MQ=255)

              cTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACaaa                                                                              >  1:4050792/1‑93 (MQ=255)

               tGCAGAGAGTGTTGTTGACGCCATGGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACaaaa                                                                             <  2:3554821/93‑1 (MQ=255)

                gCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACaaaaa                                                                            <  1:5592191/93‑1 (MQ=255)

                gCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACaaaaa                                                                            <  1:3070607/93‑1 (MQ=255)

                gCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACaaaaa                                                                            <  1:2279548/93‑1 (MQ=255)

                gCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACaaaaa                                                                            <  2:3587389/93‑1 (MQ=255)

                  agagagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATg                                                                          >  2:1352238/1‑93 (MQ=255)

                  agagagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATg                                                                          <  1:4612252/93‑1 (MQ=255)

                   gagagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAAttc                                                                         >  2:271865/1‑91 (MQ=255)

                   gagagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGc                                                                         >  1:3791042/1‑93 (MQ=255)

                   gagagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGc                                                                         >  1:2720668/1‑93 (MQ=255)

                   gagagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGc                                                                         <  1:1078581/93‑1 (MQ=255)

                    agcgtggtgTTGACGCCATTGAACGCGCGAAGCACGCCGCCGTACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGcc                                                                        <  2:219676/90‑1 (MQ=255)

                    agagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGcc                                                                        <  2:5717495/93‑1 (MQ=255)

                    agagTGTTGTCGACGCCATTGAACGCGCGAAGCACGCCGCCGGAGAGATAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGcc                                                                        <  1:2189389/93‑1 (MQ=255)

                     gagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGccc                                                                       <  2:5505314/93‑1 (MQ=255)

                     gagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGccc                                                                       <  2:5131365/93‑1 (MQ=255)

                     gagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGccc                                                                       <  1:933959/93‑1 (MQ=255)

                     gagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGccc                                                                       <  2:1932247/93‑1 (MQ=255)

                     gagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGccc                                                                       >  2:439051/1‑93 (MQ=255)

                     gagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGccc                                                                       <  2:402268/93‑1 (MQ=255)

                      agTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCg                                                                      <  1:1617946/93‑1 (MQ=255)

                        tgttgtTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGAt                                                                    <  1:6168062/93‑1 (MQ=255)

                         gttgttGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATc                                                                   >  1:639569/1‑93 (MQ=255)

                          ttgttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATcc                                                                  <  2:2922997/93‑1 (MQ=255)

                            gttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTc                                                                <  2:520789/93‑1 (MQ=255)

                             ttgACGCCATTGAACGCGGGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCg                                                               <  1:2329456/93‑1 (MQ=255)

                             ttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCg                                                               >  2:4987550/1‑93 (MQ=255)

                               gACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGAt                                                             <  1:1806448/93‑1 (MQ=255)

                                aCGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATc                                                            <  2:5490589/93‑1 (MQ=255)

                                  gCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATACTCCATCgg                                                          >  2:3435060/1‑93 (MQ=255)

                                   ccATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATACCCGATCCTCGATCggg                                                         >  2:302744/1‑93 (MQ=255)

                                   ccATTGAAAGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCggg                                                         >  2:6119175/1‑93 (MQ=255)

                                     aTTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAATCCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCa                                                       <  1:3219229/93‑1 (MQ=255)

                                     aTTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAACCCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCa                                                       <  1:3698483/93‑1 (MQ=255)

                                     aTTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCa                                                       >  2:1877837/1‑93 (MQ=255)

                                     aTTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCa                                                       >  1:820908/1‑93 (MQ=255)

                                      ttGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAt                                                      <  2:2483288/93‑1 (MQ=255)

                                        gAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAttt                                                    <  2:5686781/93‑1 (MQ=255)

                                        gAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAttt                                                    <  1:1754033/93‑1 (MQ=255)

                                         aaCGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAACCCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAtttt                                                   <  2:2762313/93‑1 (MQ=255)

                                         aaCGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAtttt                                                   <  1:3080269/93‑1 (MQ=255)

                                          aCGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTg                                                  >  1:4878514/1‑93 (MQ=255)

                                            gcgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGAc                                                <  1:788173/93‑1 (MQ=255)

                                              gcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtt                                              >  1:5828456/1‑93 (MQ=255)

                                              gcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtt                                              <  1:2520726/93‑1 (MQ=255)

                                              gcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtt                                              <  2:3208777/93‑1 (MQ=255)

                                              gcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtt                                              <  1:1573837/93‑1 (MQ=255)

                                                 aaGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTa                                           <  1:1007272/93‑1 (MQ=255)

                                                 aaGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTa                                           <  1:6293889/93‑1 (MQ=255)

                                                  aGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTAc                                          >  1:1368733/1‑93 (MQ=255)

                                                  aGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTAc                                          <  2:5034182/93‑1 (MQ=255)

                                                  aGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATACTCGATCGGGCATTTTGACTTTTAc                                          >  1:4258976/1‑93 (MQ=255)

                                                   gCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACa                                         >  2:450312/1‑93 (MQ=255)

                                                   gCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACa                                         <  1:1447852/93‑1 (MQ=255)

                                                   gCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACa                                         <  1:4745681/93‑1 (MQ=255)

                                                       gccgccGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCtt                                     <  1:1848827/93‑1 (MQ=255)

                                                        ccgccgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTa                                    >  1:1337468/1‑93 (MQ=255)

                                                        ccgccgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTa                                    <  2:1760752/93‑1 (MQ=255)

                                                        ccgccgAACAGGTAAAAGTACAGGGTTAACCCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTa                                    <  2:2343826/93‑1 (MQ=255)

                                                         cgccgGACAGGTAAAATTACAGGGTTAAACCAGGC‑AAAAAACGCTACAAAGATGCCGGATCCTCGATCGGGCGTTTTGACTTTTACAGCTTAg                                   <  1:3388078/93‑1 (MQ=255)

                                                         cgccgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAg                                   >  2:2101923/1‑93 (MQ=255)

                                                          gccgGACAGGTAAAAGTACAGGTTTAACCCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgc                                  <  1:5712025/93‑1 (MQ=255)

                                                          gccgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTTTCTTTTTTATCTTAgc                                  >  1:4381397/1‑93 (MQ=255)

                                                            cgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgcgc                                >  2:4089241/1‑93 (MQ=255)

                                                             ggACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGcc                               >  2:651519/1‑93 (MQ=255)

                                                              gACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCt                              <  2:1601320/93‑1 (MQ=255)

                                                               aCAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCtt                             >  1:5031942/1‑93 (MQ=255)

                                                                 aGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCctct                           >  2:4630508/1‑93 (MQ=255)

                                                                 aGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCt                           <  2:838865/93‑1 (MQ=255)

                                                                  ggTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTa                          >  2:3789525/1‑93 (MQ=255)

                                                                  ggTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTa                          <  1:1028766/93‑1 (MQ=255)

                                                                   gTAACAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTTCAGCTTAGAGCCTTCtcc                         >  2:5985743/1‑91 (MQ=255)

                                                                   gTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTAc                         >  2:687169/1‑93 (MQ=255)

                                                                    tAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACa                        <  1:5411155/93‑1 (MQ=255)

                                                                    tAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACa                        <  2:4894150/93‑1 (MQ=255)

                                                                    tAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCCTCTACa                        >  2:3599874/1‑93 (MQ=255)

                                                                     aaaaGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAg                       <  2:1638530/93‑1 (MQ=255)

                                                                      aaaGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGc                      <  1:3628551/93‑1 (MQ=255)

                                                                      aaaGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGc                      <  1:2861623/93‑1 (MQ=255)

                                                                       aaGTACAGGGTTAAACCCGGC‑AAAAACCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCt                     <  1:5329970/93‑1 (MQ=255)

                                                                        aGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCtt                    >  1:5871710/1‑93 (MQ=255)

                                                                        aGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCCGGCACTTTTACTTTTTCAGCTTAGCGCCTTCTACAGCtt                    >  1:2460811/1‑93 (MQ=255)

                                                                          tACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCa                  <  1:4201398/93‑1 (MQ=255)

                                                                             aGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAcgc               >  1:4939076/1‑93 (MQ=255)

                                                                             aGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAcgc               <  2:5657709/93‑1 (MQ=255)

                                                                             aGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAcgc               <  2:6206505/93‑1 (MQ=255)

                                                                              gggTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCCATCCCCCATCGGGGCTTTTTACTTTTTTAACTTAGCGCCTTCTTCAACTTCAcccg              >  2:2282436/1‑93 (MQ=255)

                                                                               ggTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGCGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGATTCACgcgc             >  1:2983062/1‑93 (MQ=255)

                                                                                gTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGcc            >  1:4764327/1‑93 (MQ=255)

                                                                                    aaCCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCt        >  2:2051061/1‑93 (MQ=255)

                                                                                     aCCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCtt       >  1:2943082/1‑93 (MQ=255)

                                                                                      cccGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTa      >  2:3095040/1‑93 (MQ=255)

GGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAA  >  NC_000913/1929999‑1930182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTC  >  NC_000913/1930006‑1930189

CTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGC                                                                                           >  SRR4302533.5243948/1‑94 (MQ=60)

 TGGCTCCTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCT                                                                                          <  SRR4302533.51447/94‑1 (MQ=60)

 TGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCT                                                                                          <  SRR4302533.2400326/94‑1 (MQ=60)

  GGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTA                                                                                         >  SRR4302533.2268829/1‑94 (MQ=60)

   GCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTAC                                                                                        <  SRR4302533.1072224/94‑1 (MQ=60)

    CTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACA                                                                                       <  SRR4302533.1881562/94‑1 (MQ=60)

    CTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACA                                                                                       <  SRR4302533.2656183/94‑1 (MQ=60)

    CTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACA                                                                                       <  SRR4302533.1355482/94‑1 (MQ=60)

    CTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACA                                                                                       <  SRR4302533.5707608/94‑1 (MQ=60)

      ACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAA                                                                                     >  SRR4302533.4075468/1‑94 (MQ=60)

        TGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGACGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAA                                                                                   >  SRR4302533.62831/1‑94 (MQ=60)

         GCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAAT                                                                                  <  SRR4302533.2293665/94‑1 (MQ=60)

         GCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAAT                                                                                  <  SRR4302533.1074810/94‑1 (MQ=60)

         GCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAAT                                                                                  <  SRR4302533.5625972/94‑1 (MQ=60)

         GCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAAT                                                                                  <  SRR4302533.3089460/94‑1 (MQ=60)

          CAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATG                                                                                 >  SRR4302533.3632093/1‑94 (MQ=60)

          CAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATG                                                                                 <  SRR4302533.1044542/94‑1 (MQ=60)

           AGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGC                                                                                >  SRR4302533.2737368/1‑94 (MQ=60)

           AGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGC                                                                                >  SRR4302533.3814255/1‑94 (MQ=60)

           AGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGC                                                                                <  SRR4302533.81525/94‑1 (MQ=60)

           AGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGC                                                                                <  SRR4302533.4640195/94‑1 (MQ=60)

            GAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCC                                                                               <  SRR4302533.1084938/94‑1 (MQ=60)

            GAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCC                                                                               >  SRR4302533.1883715/1‑94 (MQ=60)

             AGAGTGTTGTCGACGCCATTGAACGCGCGAAGCACGCCGCCGGAGAGATAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCC                                                                              <  SRR4302533.2202877/94‑1 (MQ=60)

              GAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCG                                                                             <  SRR4302533.939440/94‑1 (MQ=60)

               AGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGA                                                                            >  SRR4302533.4788959/1‑94 (MQ=60)

               AGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGA                                                                            <  SRR4302533.1627749/94‑1 (MQ=60)

                GTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGAT                                                                           <  SRR4302533.5661662/94‑1 (MQ=60)

                GTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGAT                                                                           <  SRR4302533.1767743/94‑1 (MQ=60)

                 TGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATC                                                                          <  SRR4302533.6205142/94‑1 (MQ=60)

                 TGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATC                                                                          <  SRR4302533.5009851/94‑1 (MQ=60)

                 TGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATC                                                                          >  SRR4302533.643333/1‑94 (MQ=60)

                  GTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCC                                                                         <  SRR4302533.5320858/94‑1 (MQ=60)

                   TTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCT                                                                        <  SRR4302533.4240645/94‑1 (MQ=60)

                     GTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCG                                                                      <  SRR4302533.6320840/94‑1 (MQ=60)

                      TTGACGCCATTGAACGCGGGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGA                                                                     <  SRR4302533.2343878/94‑1 (MQ=60)

                       TGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGAT                                                                    <  SRR4302533.2611349/94‑1 (MQ=60)

                        GACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATC                                                                   <  SRR4302533.1817442/94‑1 (MQ=60)

                             CATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCA                                                              >  SRR4302533.825722/1‑94 (MQ=60)

                              ATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAATCCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAT                                                             <  SRR4302533.3239079/94‑1 (MQ=60)

                              ATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAACCCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAT                                                             <  SRR4302533.3721199/94‑1 (MQ=60)

                              ATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCCAGCCTCGATCGGGGAT                                                             >  SRR4302533.5475643/1‑94 (MQ=60)

                                TGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTT                                                           >  SRR4302533.1877402/1‑94 (MQ=60)

                                 GAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTT                                                          <  SRR4302533.1764717/94‑1 (MQ=60)

                                  AACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTG                                                         <  SRR4302533.3099167/94‑1 (MQ=60)

                                  AACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTG                                                         >  SRR4302533.4908068/1‑94 (MQ=60)

                                  AACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTG                                                         >  SRR4302533.4908067/1‑94 (MQ=60)

                                    CGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGAC                                                       >  SRR4302533.4754131/1‑94 (MQ=60)

                                     GCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACT                                                      <  SRR4302533.792804/94‑1 (MQ=60)

                                     GCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCCATCGGGCATTTTGACT                                                      >  SRR4302533.23347/1‑94 (MQ=60)

                                      CGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTT                                                     >  SRR4302533.5863673/1‑94 (MQ=60)

                                      CGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGGTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTTACTT                                                     >  SRR4302533.5724081/1‑94 (MQ=60)

                                       GCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTT                                                    <  SRR4302533.1583314/94‑1 (MQ=60)

                                       GCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTT                                                    <  SRR4302533.2536257/94‑1 (MQ=60)

                                        CGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTT                                                   >  SRR4302533.1660972/1‑94 (MQ=60)

                                          AAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTAC                                                 <  SRR4302533.1013164/94‑1 (MQ=60)

                                          AAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTAC                                                 >  SRR4302533.1376962/1‑94 (MQ=60)

                                          AAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTAC                                                 <  SRR4302533.6331409/94‑1 (MQ=60)

                                          AAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATACTCGATCGGGCATTTTGACTTTTAC                                                 >  SRR4302533.4284645/1‑94 (MQ=60)

                                            GCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAG                                               <  SRR4302533.4774454/94‑1 (MQ=60)

                                            GCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAG                                               <  SRR4302533.1456587/94‑1 (MQ=60)

                                              CCGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCT                                             <  SRR4302533.1824150/94‑1 (MQ=60)

                                               CGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTT                                            <  SRR4302533.3806850/94‑1 (MQ=60)

                                                GCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTA                                           <  SRR4302533.1860104/94‑1 (MQ=60)

                                                GCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTA                                           >  SRR4302533.1345493/1‑94 (MQ=60)

                                                GCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTA                                           <  SRR4302533.648253/94‑1 (MQ=60)

                                                GCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTA                                           >  SRR4302533.5860329/1‑94 (MQ=60)

                                                 CCGCCGGACAGATAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAG                                          <  SRR4302533.3707998/94‑1 (MQ=60)

                                                  CGCCGGACAGGTAAAATTACAGGGTTAAACCAGGC‑AAAAAACGCTACAAAGATGCCGGATCCTCGATCGGGCGTTTTGACTTTTACAGCTTAGC                                         <  SRR4302533.3408998/94‑1 (MQ=60)

                                                  CGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTttcttttttatcttagc                                         >  SRR4302533.4407851/1‑77 (MQ=60)

                                                  CGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGC                                         >  SRR4302533.3089974/1‑94 (MQ=60)

                                                   GCCGGACAGGTAAAAGTACAGGTTTAACCCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCG                                        <  SRR4302533.5746528/94‑1 (MQ=60)

                                                      cgaacagtaaaaagtccagggTAAACCCGGGAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCC                                      <  SRR4302533.1217788/73‑1 (MQ=60)

                                                      cgaacagtaaaaagtacagggtaaaacccaaAAAAAAACCCTACAAAACTGCCCGATCCTCGCCGGGCCATTTTGACTTTTACAGCTTAGCGCC                                      <  SRR4302533.1121580/63‑1 (MQ=30)

                                                      GGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCT                                     >  SRR4302533.3969939/1‑94 (MQ=60)

                                                      GGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCT                                     >  SRR4302533.2978382/1‑94 (MQ=60)

                                                       GACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTT                                    >  SRR4302533.5062408/1‑94 (MQ=60)

                                                        ACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTC                                   >  SRR4302533.964556/1‑94 (MQ=60)

                                                          AGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTA                                 <  SRR4302533.4355517/94‑1 (MQ=60)

                                                           GGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGGCCGATCCTCGAACGGGCATTTTGACTTTTAtaagtttacgccttctac                                >  SRR4302533.5419873/1‑76 (MQ=60)

                                                           GGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTAC                                <  SRR4302533.1034807/94‑1 (MQ=60)

                                                             TAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAG                              <  SRR4302533.1600689/94‑1 (MQ=60)

                                                             TAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAG                              <  SRR4302533.5443690/94‑1 (MQ=60)

                                                             TAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAG                              >  SRR4302533.1483067/1‑94 (MQ=60)

                                                               AAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCT                            <  SRR4302533.2879226/94‑1 (MQ=60)

                                                               AAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCT                            <  SRR4302533.3650879/94‑1 (MQ=60)

                                                               AAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCT                            >  SRR4302533.4212088/1‑94 (MQ=60)

                                                                AAGTACAGGGTTAAACCCGGC‑AAAAACCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTT                           <  SRR4302533.5361915/94‑1 (MQ=60)

                                                                AAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTT                           >  SRR4302533.5907201/1‑94 (MQ=60)

                                                                AAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCCGGCACTTTTACTTTTTCAGCTTAGCGCCTTCTACAGCTT                           >  SRR4302533.2475996/1‑94 (MQ=60)

                                                                 AGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATTCCCGATCCTCGATCGGGGCGTTTGGATTTTACAACTTAGCGCCTTCTATAGCTTC                          >  SRR4302533.2485952/1‑94 (MQ=48)

                                                                 AGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCCATCCGGCAATTTTACTTTTTAAGCTTAGCGCCCTCTACAACTTC                          >  SRR4302533.4474536/1‑94 (MQ=48)

                                                                  GTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCA                         <  SRR4302533.6185994/94‑1 (MQ=60)

                                                                  GTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAAGGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCA                         <  SRR4302533.520856/94‑1 (MQ=60)

                                                                   TACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAC                        <  SRR4302533.4226820/94‑1 (MQ=60)

                                                                   TACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAC                        >  SRR4302533.5905448/1‑94 (MQ=60)

                                                                     CAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGC                      >  SRR4302533.4968975/1‑94 (MQ=60)

                                                                     CAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATAGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGC                      >  SRR4302533.4968974/1‑94 (MQ=60)

                                                                      AGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCG                     >  SRR4302533.2677085/1‑94 (MQ=60)

                                                                       GGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGCGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGATTCACGCGC                    >  SRR4302533.3001309/1‑94 (MQ=60)

                                                                        GGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCC                   >  SRR4302533.4793220/1‑94 (MQ=60)

                                                                            AAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCT               <  SRR4302533.3068612/94‑1 (MQ=60)

                                                                             AACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTT              >  SRR4302533.2961048/1‑94 (MQ=60)

                                                                              ACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTA             >  SRR4302533.923059/1‑94 (MQ=60)

                                                                               CCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAG            <  SRR4302533.3172696/94‑1 (MQ=60)

                                                                               CCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAG            <  SRR4302533.1418531/94‑1 (MQ=60)

                                                                               CCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACATTTACCGCTTAGCGCCCTCTACAGCTTCACGCGCCAGCTTAG            >  SRR4302533.384286/1‑94 (MQ=60)

                                                                                CCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGT           <  SRR4302533.2803965/94‑1 (MQ=60)

                                                                                CCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGT           >  SRR4302533.3038092/1‑94 (MQ=60)

                                                                                CCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGT           >  SRR4302533.5886044/1‑94 (MQ=60)

                                                                                 CGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTA          >  SRR4302533.3025717/1‑94 (MQ=60)

                                                                                 CGGC‑AAAAAACGCTACAAAAATGCCCGAACCTCGATCCGGCATTTTtgctttttaagcttatagccttcttcagactcacccgccaccttagta          >  SRR4302533.1224997/1‑46 (MQ=60)

                                                                                    gcAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT        <  SRR4302533.865909/92‑1 (MQ=60)

                                                                                     CAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATG       <  SRR4302533.3922884/94‑1 (MQ=60)

                                                                                      AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGC      >  SRR4302533.1006334/1‑94 (MQ=60)

                                                                                        AAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGG    <  SRR4302533.5464901/94‑1 (MQ=60)

                                                                                        AAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGG    >  SRR4302533.1272105/1‑94 (MQ=60)

                                                                                         AAACGCTACAAAAATGCCCGATCCTCGATCGGGGATTTTTACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGT   >  SRR4302533.4953302/1‑94 (MQ=60)

                                                                                         AAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGT   <  SRR4302533.5047893/94‑1 (MQ=60)

                                                                                         AAACGCAACAGAAATGCCGGACCCTCGATCGGCCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGT   <  SRR4302533.5446014/94‑1 (MQ=60)

                                                                                          AACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTC  <  SRR4302533.555207/94‑1 (MQ=60)

                                                                                          AACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTC  <  SRR4302533.5490589/94‑1 (MQ=60)

                                                                                          AACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTC  <  SRR4302533.3860588/94‑1 (MQ=60)

                                                                                          AACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCAACAGCTTCACGCGCCAGCTTAGTAATGCGGTC  <  SRR4302533.6234321/94‑1 (MQ=60)

CTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTC  >  NC_000913/1930006‑1930189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`