Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,081,054	A→G	T93A (ACC→GCC)	sbcB →	exonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,081,054	0	A	G	100.0%	630.1 / NA	196	T93A (ACC→GCC)	sbcB	exonuclease I
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (86/110); total (86/110)

Only 100 of 221 total aligned reads displayed.

GCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGA  >  NC_000913/2080964‑2081142

  cGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgt                                                                                      >  1:5314805/1‑93 (MQ=255)

  cGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgt                                                                                      >  1:2248008/1‑93 (MQ=255)

    tATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgtac                                                                                    >  2:3992792/1‑93 (MQ=255)

    tATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgtac                                                                                    >  1:3714403/1‑93 (MQ=255)

    tATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgtac                                                                                    >  1:2721909/1‑93 (MQ=255)

      ttaattaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgtaccg                                                                                  >  2:5324525/1‑93 (MQ=255)

      ttaattaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgtaccg                                                                                  <  1:4765124/93‑1 (MQ=255)

        aattaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGaa                                                                                >  2:5390328/1‑93 (MQ=255)

        aattaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGaa                                                                                >  2:230198/1‑93 (MQ=255)

             ccGTTATTCCCCCGCAGAAAGCACGGCCAAAAGGAGAAAACG‑AGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTg                                                                          <  2:4164483/93‑1 (MQ=255)

              cGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTg                                                                          <  2:4283797/93‑1 (MQ=255)

               ggTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGt                                                                         >  2:62257/1‑93 (MQ=255)

                gTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTa                                                                        <  2:705877/93‑1 (MQ=255)

                 tATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTAt                                                                       <  2:4584716/93‑1 (MQ=255)

                   ttACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTc                                                                     <  1:1170388/93‑1 (MQ=255)

                   ttACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTc                                                                     <  2:4761044/93‑1 (MQ=255)

                    tACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGCAGACCTGTATTCt                                                                    <  2:6193572/93‑1 (MQ=255)

                    tACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCt                                                                    <  1:4852693/93‑1 (MQ=255)

                    tACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCt                                                                    <  1:4947211/93‑1 (MQ=255)

                    tACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCt                                                                    >  1:3487109/1‑93 (MQ=255)

                      ccccGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTgg                                                                  <  2:5867804/93‑1 (MQ=255)

                       cccGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTggg                                                                 >  1:1393833/1‑93 (MQ=255)

                       cccGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGGTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGGATTCTggg                                                                 >  2:1770598/1‑93 (MQ=255)

                         cGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCt                                                               >  1:663313/1‑93 (MQ=255)

                          gCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTa                                                              >  1:4515303/1‑93 (MQ=255)

                          gCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTa                                                              >  1:1235361/1‑93 (MQ=255)

                          gCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTa                                                              >  1:3569395/1‑93 (MQ=255)

                           cAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTac                                                             <  1:1298358/93‑1 (MQ=255)

                            aGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTaca                                                            <  1:2237511/93‑1 (MQ=255)

                             ggAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaa                                                           >  1:6152875/1‑93 (MQ=255)

                             ggAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaa                                                           <  1:5035414/93‑1 (MQ=255)

                             ggAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaa                                                           <  1:4269325/93‑1 (MQ=255)

                             ggAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaa                                                           <  1:249166/93‑1 (MQ=255)

                             ggAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaa                                                           >  1:460879/1‑93 (MQ=255)

                              gAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaac                                                          <  2:499755/93‑1 (MQ=255)

                              gAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaac                                                          >  2:3805598/1‑93 (MQ=255)

                              gAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaac                                                          >  1:1766362/1‑93 (MQ=255)

                                aGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTAcaacaa                                                        <  1:4099686/93‑1 (MQ=255)

                                aGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTAcaacaa                                                        <  2:5808968/93‑1 (MQ=255)

                                  cACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAAtg                                                      <  1:2835651/93‑1 (MQ=255)

                                  cACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAAtg                                                      <  2:5665375/93‑1 (MQ=255)

                                   aCGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAAtgt                                                     <  2:3086811/93‑1 (MQ=255)

                                     gggCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCTCTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGc                                                   <  1:620721/93‑1 (MQ=255)

                                         gAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGttt                                               <  2:3606125/93‑1 (MQ=255)

                                         gAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGttt                                               >  1:5776615/1‑93 (MQ=255)

                                          aaaGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTc                                              >  2:5128741/1‑93 (MQ=255)

                                          aaaGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTc                                              >  2:1349359/1‑93 (MQ=255)

                                           aaGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcg                                             >  1:3962897/1‑93 (MQ=255)

                                           aaGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcg                                             >  1:2565260/1‑93 (MQ=255)

                                            aGGAAAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcga                                            <  2:1328583/93‑1 (MQ=255)

                                             ggAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgac                                           <  2:5207161/93‑1 (MQ=255)

                                              gagaAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacg                                          >  2:29894/1‑93 (MQ=255)

                                               agaAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacga                                         <  1:2312942/93‑1 (MQ=255)

                                                gaAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacgac                                        <  2:306220/93‑1 (MQ=255)

                                                 aaaaCGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacgacg                                       <  1:5907142/93‑1 (MQ=255)

                                                  aaaCGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacgacga                                      >  1:3785856/1‑93 (MQ=255)

                                                  aaaCGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacgacga                                      >  2:657406/1‑93 (MQ=255)

                                                  aaaCGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacgacga                                      <  1:1626379/93‑1 (MQ=255)

                                                  aaaCGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacgacga                                      <  2:2012591/93‑1 (MQ=255)

                                                     cGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGt                                   <  1:1549738/93‑1 (MQ=255)

                                                      gAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTc                                  >  2:5651965/1‑93 (MQ=255)

                                                      gAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTc                                  <  2:6060819/93‑1 (MQ=255)

                                                       aaGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTca                                 >  2:525371/1‑93 (MQ=255)

                                                          ccGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCacac                              <  1:2060916/93‑1 (MQ=255)

                                                           cgcgTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACg                             <  2:4802544/93‑1 (MQ=255)

                                                            gcgTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGc                            <  1:2150002/93‑1 (MQ=255)

                                                              gTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCac                          >  1:5805954/1‑92 (MQ=255)

                                                              gTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCaa                          <  2:5509333/93‑1 (MQ=255)

                                                               tttGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTGTTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAAc                         >  1:4089854/1‑93 (MQ=255)

                                                               tttGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAAc                         >  1:5797771/1‑93 (MQ=255)

                                                                  gccgccCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAtt                      >  1:886292/1‑93 (MQ=255)

                                                                     gccCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAttttt                   >  2:1594666/1‑93 (MQ=255)

                                                                     gccCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAttttt                   >  2:1939196/1‑93 (MQ=255)

                                                                      cccGTATTCCCTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAtttttt                  <  2:4837073/93‑1 (MQ=255)

                                                                       ccGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTa                 <  2:1855903/93‑1 (MQ=255)

                                                                       ccGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTa                 >  1:3371075/1‑93 (MQ=255)

                                                                       ccGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTa                 <  1:3144105/93‑1 (MQ=255)

                                                                       ccGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGGTTCGACGACGAAGTCACACGCAACATTTTTTa                 <  2:2696187/93‑1 (MQ=255)

                                                                         gTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATc               <  1:3824506/93‑1 (MQ=255)

                                                                         gTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATc               >  1:4485385/1‑93 (MQ=255)

                                                                         gTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATc               >  2:4395605/1‑93 (MQ=255)

                                                                         gTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATc               >  2:3165697/1‑93 (MQ=255)

                                                                          tATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCg              <  1:1009937/93‑1 (MQ=255)

                                                                          tATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCg              >  1:1342268/1‑93 (MQ=255)

                                                                           aTTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGt             <  2:34536/93‑1 (MQ=255)

                                                                            ttCACTCGCTTTTTGGCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTa            <  2:4243984/93‑1 (MQ=255)

                                                                            ttCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTa            >  1:2730823/1‑93 (MQ=255)

                                                                            ttCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTa            <  1:5509971/93‑1 (MQ=255)

                                                                             tCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTaa           <  2:2444544/93‑1 (MQ=255)

                                                                              cACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAt          >  2:1399625/1‑93 (MQ=255)

                                                                              cACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAt          <  2:3303309/93‑1 (MQ=255)

                                                                               aCTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAtt         >  2:2411635/1‑93 (MQ=255)

                                                                               aCTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAtt         >  2:383397/1‑93 (MQ=255)

                                                                                cTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAttt        <  1:5362593/93‑1 (MQ=255)

                                                                                 tCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTc       <  1:1860534/93‑1 (MQ=255)

                                                                                   gCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTa     <  1:5617004/93‑1 (MQ=255)

                                                                                    cTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTAc    <  1:3421111/93‑1 (MQ=255)

                                                                                    cTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTAc    >  2:4076461/1‑93 (MQ=255)

                                                                                      ttttGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGa  >  1:3177042/1‑93 (MQ=255)

                                                                                      ttttGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGa  <  1:918723/93‑1 (MQ=255)

GCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGA  >  NC_000913/2080964‑2081142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTT  >  NC_000913/2080961‑2081147

GGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTG                                                                                               <  SRR4302533.119443/94‑1 (MQ=60)

 GAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGC                                                                                              >  SRR4302533.925137/1‑94 (MQ=60)

  AGCCGTATTAATTACCGGTATTGCCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCC                                                                                             <  SRR4302533.2077448/94‑1 (MQ=60)

  AGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCC                                                                                             <  SRR4302533.1187120/94‑1 (MQ=60)

  AGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCC                                                                                             <  SRR4302533.5510839/94‑1 (MQ=60)

  AGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCC                                                                                             >  SRR4302533.5105624/1‑94 (MQ=60)

  AGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCC                                                                                             <  SRR4302533.225295/94‑1 (MQ=60)

   GCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCG                                                                                            >  SRR4302533.857920/1‑94 (MQ=60)

   GCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCG                                                                                            <  SRR4302533.4799165/94‑1 (MQ=60)

    CCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGT                                                                                           >  SRR4302533.5346654/1‑94 (MQ=60)

    CCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGT                                                                                           >  SRR4302533.2261901/1‑94 (MQ=60)

    CCGTATTAATTACCGGTATCCCCCCGCAGCAAGCACGGGCGAAAGGAGAAAACGAACCCGCGTTTGCCGCCCGTATTCCCTCGCTTTTTGCCGT                                                                                           <  SRR4302533.3701177/94‑1 (MQ=60)

     CGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTA                                                                                          >  SRR4302533.5881485/1‑94 (MQ=60)

     CGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTA                                                                                          >  SRR4302533.581436/1‑94 (MQ=60)

      GTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTAC                                                                                         >  SRR4302533.3737208/1‑94 (MQ=60)

      GTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTAC                                                                                         >  SRR4302533.2738613/1‑94 (MQ=60)

       TATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACC                                                                                        >  SRR4302533.6281630/1‑94 (MQ=60)

        ATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCG                                                                                       >  SRR4302533.3744448/1‑94 (MQ=60)

         TTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGA                                                                                      <  SRR4302533.240940/94‑1 (MQ=60)

         TTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGA                                                                                      <  SRR4302533.4794021/94‑1 (MQ=60)

          TAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAA                                                                                     <  SRR4302533.5016178/94‑1 (MQ=60)

             TTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGAC                                                                                  <  SRR4302533.3519887/94‑1 (MQ=60)

               ACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCT                                                                                <  SRR4302533.19041/94‑1 (MQ=60)

                CCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTG                                                                               >  SRR4302533.1392956/1‑94 (MQ=60)

                    TATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATT                                                                           >  SRR4302533.174957/1‑94 (MQ=60)

                    TATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATT                                                                           <  SRR4302533.2255497/94‑1 (MQ=60)

                     ATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTC                                                                          >  SRR4302533.564238/1‑94 (MQ=60)

                      TTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCT                                                                         <  SRR4302533.1177416/94‑1 (MQ=60)

                      TTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCT                                                                         >  SRR4302533.3508611/1‑94 (MQ=60)

                      TTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCT                                                                         <  SRR4302533.4023808/94‑1 (MQ=60)

                       TACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTG                                                                        <  SRR4302533.4882100/94‑1 (MQ=60)

                       TACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTG                                                                        <  SRR4302533.4977161/94‑1 (MQ=60)

                       TACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTG                                                                        <  SRR4302533.1930996/94‑1 (MQ=60)

                       TACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTG                                                                        <  SRR4302533.3166859/94‑1 (MQ=60)

                         CCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGG                                                                      >  SRR4302533.1402233/1‑94 (MQ=60)

                         CCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGG                                                                      >  SRR4302533.4990777/1‑94 (MQ=60)

                          CCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGC                                                                     >  SRR4302533.4897277/1‑94 (MQ=60)

                           CCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCT                                                                    >  SRR4302533.667230/1‑94 (MQ=60)

                            CGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTA                                                                   >  SRR4302533.3591395/1‑94 (MQ=60)

                            CGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTA                                                                   >  SRR4302533.4542644/1‑94 (MQ=60)

                            CGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTA                                                                   >  SRR4302533.1242747/1‑94 (MQ=60)

                              CAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACA                                                                 <  SRR4302533.6190128/94‑1 (MQ=60)

                              CAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACA                                                                 <  SRR4302533.1306172/94‑1 (MQ=60)

                               AGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAA                                                                >  SRR4302533.463630/1‑94 (MQ=60)

                               AGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAA                                                                <  SRR4302533.4539921/94‑1 (MQ=60)

                               AGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAA                                                                <  SRR4302533.2251340/94‑1 (MQ=60)

                               AGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAA                                                                >  SRR4302533.6189903/1‑94 (MQ=60)

                                GGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAAC                                                               <  SRR4302533.4295025/94‑1 (MQ=60)

                                GGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAAC                                                               <  SRR4302533.1313655/94‑1 (MQ=60)

                                GGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAAC                                                               >  SRR4302533.1777116/1‑94 (MQ=60)

                                GGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAAC                                                               <  SRR4302533.5065914/94‑1 (MQ=60)

                                GGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAAC                                                               <  SRR4302533.250704/94‑1 (MQ=60)

                                 GAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACA                                                              <  SRR4302533.3907591/94‑1 (MQ=60)

                                   AGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAAT                                                            <  SRR4302533.4124637/94‑1 (MQ=60)

                                     CACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGT                                                          <  SRR4302533.2853099/94‑1 (MQ=60)

                                      ACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTG                                                         >  SRR4302533.1829556/1‑94 (MQ=60)

                                       CGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGC                                                        <  SRR4302533.3475762/94‑1 (MQ=60)

                                        GGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCTCTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCG                                                       <  SRR4302533.624368/94‑1 (MQ=60)

                                           CGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT                                                    >  SRR4302533.2518069/1‑94 (MQ=60)

                                           CGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT                                                    <  SRR4302533.5841000/94‑1 (MQ=60)

                                           CGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT                                                    >  SRR4302533.5811504/1‑94 (MQ=60)

                                           CGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT                                                    >  SRR4302533.5603359/1‑94 (MQ=60)

                                           CGAAAGAAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT                                                    <  SRR4302533.1489484/94‑1 (MQ=60)

                                            GAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTC                                                   >  SRR4302533.3701987/1‑94 (MQ=60)

                                            GAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTC                                                   <  SRR4302533.2300424/94‑1 (MQ=60)

                                            GAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTC                                                   >  SRR4302533.3374772/1‑94 (MQ=60)

                                             AAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCG                                                  >  SRR4302533.3987039/1‑94 (MQ=60)

                                             AAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCG                                                  >  SRR4302533.2581047/1‑94 (MQ=60)

                                               AGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGAC                                                >  SRR4302533.3834330/1‑94 (MQ=60)

                                               AGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGAC                                                <  SRR4302533.4563076/94‑1 (MQ=60)

                                                  AGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGAC                                             <  SRR4302533.2327270/94‑1 (MQ=60)

                                                   GAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACG                                            <  SRR4302533.3476392/94‑1 (MQ=60)

                                                   GAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACG                                            >  SRR4302533.2935586/1‑94 (MQ=60)

                                                    AAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGA                                           >  SRR4302533.3809035/1‑94 (MQ=60)

                                                    AAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGA                                           <  SRR4302533.5942813/94‑1 (MQ=60)

                                                     AAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAA                                          <  SRR4302533.5989323/94‑1 (MQ=60)

                                                     AAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAA                                          <  SRR4302533.1636228/94‑1 (MQ=60)

                                                     AAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAA                                          >  SRR4302533.2468717/1‑94 (MQ=60)

                                                      AACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAG                                         <  SRR4302533.5656230/94‑1 (MQ=60)

                                                       ACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGT                                        <  SRR4302533.41637/94‑1 (MQ=60)

                                                        CGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTC                                       <  SRR4302533.1559077/94‑1 (MQ=60)

                                                            GCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACAC                                   <  SRR4302533.2663858/94‑1 (MQ=60)

                                                             CCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACG                                  >  SRR4302533.4515610/1‑94 (MQ=60)

                                                             CCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACG                                  <  SRR4302533.2073627/94‑1 (MQ=60)

                                                             CCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACG                                  <  SRR4302533.4115720/94‑1 (MQ=60)

                                                              CGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGC                                 >  SRR4302533.4652046/1‑94 (MQ=60)

                                                              CGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGACTTCTGGCCTACAACAATGTGCGGTTCGACGACGAGATCACACGC                                 >  SRR4302533.448746/1‑94 (MQ=60)

                                                               GCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCA                                <  SRR4302533.2163242/94‑1 (MQ=60)

                                                                CGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAC                               >  SRR4302533.5841014/1‑94 (MQ=60)

                                                                 GTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTGTTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAAC                              >  SRR4302533.4114752/1‑94 (MQ=60)

                                                                 GTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAAC                              >  SRR4302533.5832797/1‑94 (MQ=60)

                                                                 GTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAAC                              <  SRR4302533.1265677/94‑1 (MQ=60)

                                                                    TGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATT                           >  SRR4302533.891496/1‑94 (MQ=60)

                                                                      CCGCCCGTATTCCCTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTT                         <  SRR4302533.2859891/94‑1 (MQ=60)

                                                                         CCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTA                      <  SRR4302533.5366367/94‑1 (MQ=60)

                                                                         CCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTA                      >  SRR4302533.3391893/1‑94 (MQ=60)

                                                                         CCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTA                      >  SRR4302533.137361/1‑94 (MQ=60)

                                                                          CCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAGCAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAT                     <  SRR4302533.3396435/94‑1 (MQ=60)

                                                                          CCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAT                     <  SRR4302533.3163436/94‑1 (MQ=60)

                                                                           CGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATC                    >  SRR4302533.4512541/1‑94 (MQ=60)

                                                                            GTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCG                   >  SRR4302533.1350349/1‑94 (MQ=60)

                                                                            GTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCG                   <  SRR4302533.3847889/94‑1 (MQ=60)

                                                                             TATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGT                  <  SRR4302533.1015843/94‑1 (MQ=60)

                                                                              ATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTA                 >  SRR4302533.2747574/1‑94 (MQ=60)

                                                                               TTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAA                <  SRR4302533.2128710/94‑1 (MQ=60)

                                                                               TTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAA                <  SRR4302533.5543234/94‑1 (MQ=60)

                                                                                 CACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATT              <  SRR4302533.1220230/94‑1 (MQ=60)

                                                                                 CACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATT              >  SRR4302533.2184972/1‑94 (MQ=60)

                                                                                 CACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATT              <  SRR4302533.5972659/94‑1 (MQ=60)

                                                                                 CACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATT              >  SRR4302533.2742526/1‑94 (MQ=60)

                                                                                   CTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTC            <  SRR4302533.5394800/94‑1 (MQ=60)

                                                                                   CTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTC            <  SRR4302533.1051838/94‑1 (MQ=60)

                                                                                    TCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCT           <  SRR4302533.1871880/94‑1 (MQ=60)

                                                                                     CGCTTTTTGCCGTACCGAGGACCTGTATTCTGGGATACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTA          <  SRR4302533.5394829/94‑1 (MQ=60)

                                                                                     CGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTA          <  SRR4302533.895570/94‑1 (MQ=60)

                                                                                      GCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTAC         <  SRR4302533.5650958/94‑1 (MQ=60)

                                                                                      GCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTAC         <  SRR4302533.2836652/94‑1 (MQ=60)

                                                                                       CTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACG        <  SRR4302533.3442249/94‑1 (MQ=60)

                                                                                       CTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACG        <  SRR4302533.3875124/94‑1 (MQ=60)

                                                                                       CTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACG        <  SRR4302533.1575008/94‑1 (MQ=60)

                                                                                        TTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGA       >  SRR4302533.3196581/1‑94 (MQ=60)

                                                                                         TTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGAT      <  SRR4302533.924121/94‑1 (MQ=60)

                                                                                           TTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCC    <  SRR4302533.4718001/94‑1 (MQ=60)

                                                                                             GCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTT  <  SRR4302533.694386/94‑1 (MQ=60)

GGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTT  >  NC_000913/2080961‑2081147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`