Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,088,609	T→C	P132P (CCT→CCC)	hisG →	ATP phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,088,609	0	T	C	99.5%	704.6 / ‑4.7	219	P132P (CCT→CCC)	hisG	ATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (112/106); total (113/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

Only 100 of 223 total aligned reads displayed.

CGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGG  >  NC_000913/2088519‑2088698

 ggcTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCAc                                                                                        >  1:4854967/1‑93 (MQ=255)

  gcTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCAcc                                                                                       <  2:5674962/93‑1 (MQ=255)

      tcgtcTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCt                                                                                   >  1:1564532/1‑93 (MQ=255)

      tcgtcTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCt                                                                                   >  2:9166017/1‑93 (MQ=255)

       ctgcTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTc                                                                                  <  2:7404102/90‑1 (MQ=255)

        gtcTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCa                                                                                 >  2:70745/1‑93 (MQ=255)

         tcTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCaa                                                                                >  1:2517698/1‑93 (MQ=255)

         tcTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATAGCCACCTCTTATCCCCACCTGCTCaa                                                                                >  2:7221592/1‑93 (MQ=255)

          cTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAg                                                                               <  1:9280965/93‑1 (MQ=255)

           tttCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGc                                                                              >  2:9335205/1‑93 (MQ=255)

           tttCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGc                                                                              <  1:8584409/93‑1 (MQ=255)

            ttCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCg                                                                             >  2:6242387/1‑93 (MQ=255)

            ttCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCg                                                                             >  2:3582328/1‑93 (MQ=255)

             tCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGt                                                                            <  1:7215896/93‑1 (MQ=255)

             tCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGt                                                                            <  2:682395/93‑1 (MQ=255)

             tCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGt                                                                            <  1:4745388/93‑1 (MQ=255)

             tCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGt                                                                            <  2:6762782/93‑1 (MQ=255)

                cTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTAt                                                                         <  2:580018/93‑1 (MQ=255)

                cTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTAt                                                                         >  2:1826649/1‑93 (MQ=255)

                  ggCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCCAGCGTTAtcc                                                                       >  2:2940513/1‑92 (MQ=255)

                  ggCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTAtct                                                                       >  2:147104/1‑93 (MQ=255)

                      aCGCCGGTTGATGGAGCCTGGGACGGTCCGCTCTCCTTAACCGGTAACCGTATCGCCCCCTCTTATCCCCACCTGCTCAAGCGTTATCTCGAc                                                                   <  2:8026162/93‑1 (MQ=255)

                       cGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGAcc                                                                  <  1:9266074/93‑1 (MQ=255)

                       cGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGAcc                                                                  <  1:7776811/93‑1 (MQ=255)

                         ccGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAg                                                                <  1:4433793/93‑1 (MQ=255)

                          cGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGa                                                               >  2:7006773/1‑93 (MQ=255)

                          cGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGa                                                               >  2:10037631/1‑93 (MQ=255)

                          cGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGa                                                               <  2:4288204/93‑1 (MQ=255)

                            gTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGaaa                                                             >  1:9596972/1‑93 (MQ=255)

                            gTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGaaa                                                             >  1:9596970/1‑93 (MQ=255)

                            gTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTAGACCAGaaa                                                             >  2:4531293/1‑93 (MQ=255)

                             ttGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTAGCCCCACCTGCTCAAGCTTTATCTCGACCAGAAAg                                                            <  1:1436772/93‑1 (MQ=255)

                               gatgaAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGc                                                          >  2:4070118/1‑93 (MQ=255)

                                atgaAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCa                                                         <  2:5573368/93‑1 (MQ=255)

                                 tgaAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCAt                                                        >  2:4846640/1‑93 (MQ=255)

                                 tgaAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCAt                                                        <  1:6946956/93‑1 (MQ=255)

                                  gaAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCAtc                                                       >  1:8132878/1‑93 (MQ=255)

                                   aaGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCAtct                                                      >  2:5339952/1‑93 (MQ=255)

                                      ccTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCtt                                                   >  2:6154077/1‑93 (MQ=255)

                                       cTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCttt                                                  <  2:5797161/93‑1 (MQ=255)

                                       cTGGAACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCttt                                                  <  2:902270/93‑1 (MQ=255)

                                        tGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGCACAGAAAGGCATCTCtttt                                                 >  1:598544/1‑93 (MQ=255)

                                         gggACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTa                                                <  1:8989712/93‑1 (MQ=255)

                                         gggACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTa                                                >  1:3131945/1‑93 (MQ=255)

                                         gggACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTa                                                >  1:7886135/1‑93 (MQ=255)

                                           gACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTaaa                                              <  2:646699/93‑1 (MQ=255)

                                               gTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCt                                          <  2:2445142/93‑1 (MQ=255)

                                               gTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCt                                          <  2:9399256/93‑1 (MQ=255)

                                               gTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCt                                          >  1:6023179/1‑93 (MQ=255)

                                               gTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCt                                          >  1:7079603/1‑93 (MQ=255)

                                               gTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCt                                          <  1:7032216/93‑1 (MQ=255)

                                                tCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTg                                         <  2:6848361/93‑1 (MQ=255)

                                                 ccGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGc                                        >  2:8345071/1‑93 (MQ=255)

                                                   gCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCtt                                      >  2:2830251/1‑93 (MQ=255)

                                                   gCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCtt                                      >  1:843053/1‑93 (MQ=255)

                                                   gCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCtt                                      >  1:4005758/1‑93 (MQ=255)

                                                    ctctCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTa                                     <  1:2640595/93‑1 (MQ=255)

                                                    ctctCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTa                                     >  2:3760044/1‑93 (MQ=255)

                                                    ctctCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTa                                     <  2:6572093/93‑1 (MQ=255)

                                                    ctctCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTa                                     <  1:7648120/93‑1 (MQ=255)

                                                      ctcCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACt                                   >  1:2786299/1‑93 (MQ=255)

                                                      ctcCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACt                                   >  1:3154149/1‑93 (MQ=255)

                                                         cTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGaa                                <  2:1703979/93‑1 (MQ=255)

                                                         cTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGaa                                <  1:4312579/93‑1 (MQ=255)

                                                         cTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGaa                                <  1:5165510/93‑1 (MQ=255)

                                                          ttAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGaag                               >  1:3425299/1‑92 (MQ=255)

                                                           tAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACg                              >  2:4635171/1‑93 (MQ=255)

                                                           tAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACg                              >  1:2256529/1‑93 (MQ=255)

                                                            aaaCGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATGTCTTTTAAATCCTGCTTACTGAACgg                             <  2:5047739/93‑1 (MQ=255)

                                                            aaaCGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACgg                             >  1:387399/1‑93 (MQ=255)

                                                            aaaCGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACgg                             <  1:5037735/93‑1 (MQ=255)

                                                             aaCGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGt                            <  2:3165274/93‑1 (MQ=255)

                                                               cGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTc                          >  2:4251456/1‑93 (MQ=255)

                                                                ggTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCt                         <  1:10018942/93‑1 (MQ=255)

                                                                 gTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGATCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTg                        <  1:6434148/93‑1 (MQ=255)

                                                                    aaCGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTg                     <  2:7848761/93‑1 (MQ=255)

                                                                    aaCGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTg                     >  2:1051734/1‑93 (MQ=255)

                                                                     aCGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGa                    <  2:3705692/93‑1 (MQ=255)

                                                                     aCGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGa                    <  2:1983362/93‑1 (MQ=255)

                                                                     aCGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGa                    <  2:8575828/93‑1 (MQ=255)

                                                                     aCGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGa                    <  2:5928014/93‑1 (MQ=255)

                                                                        tATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGt                 <  2:691002/93‑1 (MQ=255)

                                                                        tATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGt                 >  1:4704924/1‑93 (MQ=255)

                                                                         aTCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTc                <  2:493427/93‑1 (MQ=255)

                                                                         aTCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTc                >  2:2253360/1‑93 (MQ=255)

                                                                            gCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGCTGAAGTCGcc             <  2:3126044/93‑1 (MQ=255)

                                                                             ccACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGccc            >  1:5413192/1‑93 (MQ=255)

                                                                             ccACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGccc            >  1:4940607/1‑93 (MQ=255)

                                                                              cACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGcccc           >  1:6261925/1‑93 (MQ=255)

                                                                              cACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGcccc           >  2:8542785/1‑93 (MQ=255)

                                                                                ccTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcgc         >  1:9734882/1‑93 (MQ=255)

                                                                                 ctctTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcgcg        <  1:8021702/93‑1 (MQ=255)

                                                                                 ctctTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcgcg        <  2:8200336/93‑1 (MQ=255)

                                                                                 ctctTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcgcg        >  2:9121111/1‑93 (MQ=255)

                                                                                 ctctTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcgcg        >  1:8499477/1‑93 (MQ=255)

                                                                                  tctTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGt       >  2:8300522/1‑93 (MQ=255)

                                                                                    ttATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGc     <  1:5572483/93‑1 (MQ=255)

                                                                                     tATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGcc    <  2:8503107/93‑1 (MQ=255)

                                                                                      aTCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCg   >  2:411838/1‑93 (MQ=255)

                                                                                      aTCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCg   >  1:7290685/1‑93 (MQ=255)

CGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGG  >  NC_000913/2088519‑2088698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTG  >  NC_000913/2088516‑2088702

CGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCC                                                                                               <  SRR4302540.4144857/94‑1 (MQ=60)

CGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCC                                                                                               >  SRR4302540.3611679/1‑94 (MQ=60)

 GGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCC                                                                                              <  SRR4302540.3353780/94‑1 (MQ=60)

   CGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCAC                                                                                            <  SRR4302540.5291264/94‑1 (MQ=60)

   CGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCAC                                                                                            >  SRR4302540.4907010/1‑94 (MQ=60)

    GGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACC                                                                                           >  SRR4302540.9381540/1‑94 (MQ=60)

     GCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCT                                                                                          >  SRR4302540.4625334/1‑94 (MQ=60)

     GCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCT                                                                                          <  SRR4302540.5738888/94‑1 (MQ=60)

      CTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTG                                                                                         <  SRR4302540.5652515/94‑1 (MQ=60)

        GTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCT                                                                                       >  SRR4302540.1581703/1‑94 (MQ=60)

           GTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAA                                                                                    >  SRR4302540.2544889/1‑94 (MQ=60)

           GTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAA                                                                                    >  SRR4302540.2607894/1‑94 (MQ=60)

            TCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAG                                                                                   >  SRR4302540.7444878/1‑94 (MQ=60)

             CTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGC                                                                                  <  SRR4302540.9391896/94‑1 (MQ=60)

              TTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCG                                                                                 <  SRR4302540.8684207/94‑1 (MQ=60)

               TTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGT                                                                                <  SRR4302540.807298/94‑1 (MQ=60)

                TCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTT                                                                               <  SRR4302540.7936147/94‑1 (MQ=60)

                TCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTT                                                                               <  SRR4302540.4796029/94‑1 (MQ=60)

                TCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTT                                                                               <  SRR4302540.7296331/94‑1 (MQ=60)

                  GCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTAT                                                                             <  SRR4302540.1785309/94‑1 (MQ=60)

                   CTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATC                                                                            <  SRR4302540.7146600/94‑1 (MQ=60)

                     GGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTC                                                                          <  SRR4302540.7081440/94‑1 (MQ=60)

                       CAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGA                                                                        >  SRR4302540.4203080/1‑94 (MQ=60)

                        AACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGAC                                                                       <  SRR4302540.4409886/94‑1 (MQ=60)

                         ACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACC                                                                      <  SRR4302540.9309694/94‑1 (MQ=60)

                         ACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACC                                                                      <  SRR4302540.5072047/94‑1 (MQ=60)

                         ACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACC                                                                      <  SRR4302540.5072048/94‑1 (MQ=60)

                          CGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCA                                                                     <  SRR4302540.9376904/94‑1 (MQ=60)

                          CGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCA                                                                     <  SRR4302540.7867725/94‑1 (MQ=60)

                            CCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGA                                                                   <  SRR4302540.4481832/94‑1 (MQ=60)

                             CGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAA                                                                  <  SRR4302540.245065/94‑1 (MQ=60)

                              GGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAA                                                                 >  SRR4302540.9710209/1‑94 (MQ=60)

                              GGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAA                                                                 >  SRR4302540.9710207/1‑94 (MQ=60)

                              GGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAA                                                                 >  SRR4302540.9710208/1‑94 (MQ=60)

                              GGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAA                                                                 >  SRR4302540.4783395/1‑94 (MQ=60)

                                TTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTAGCCCCACCTGCTCAAGCTTTATCTCGACCAGAAAGG                                                               <  SRR4302540.1452356/94‑1 (MQ=60)

                                 TGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGC                                                              >  SRR4302540.8158249/1‑94 (MQ=60)

                                  GATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGGCCAGAAAGGCA                                                             <  SRR4302540.5537693/94‑1 (MQ=60)

                                    TGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATC                                                           >  SRR4302540.8227274/1‑94 (MQ=60)

                                    TGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATC                                                           <  SRR4302540.7024271/94‑1 (MQ=60)

                                        GCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTT                                                       >  SRR4302540.2740888/1‑94 (MQ=60)

                                          CTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGCACAGAAAGGCATCTCTTTT                                                     >  SRR4302540.604966/1‑94 (MQ=60)

                                           TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTA                                                    >  SRR4302540.7978200/1‑94 (MQ=60)

                                           TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTA                                                    >  SRR4302540.2655753/1‑94 (MQ=60)

                                           TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTA                                                    >  SRR4302540.3166120/1‑94 (MQ=60)

                                            GGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAA                                                   <  SRR4302540.9097307/94‑1 (MQ=60)

                                             GGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAA                                                  >  SRR4302540.3792779/1‑94 (MQ=60)

                                              GACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAAT                                                 <  SRR4302540.6058832/94‑1 (MQ=60)

                                              GACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAAT                                                 >  SRR4302540.3181046/1‑94 (MQ=60)

                                              GACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAAT                                                 <  SRR4302540.3320589/94‑1 (MQ=60)

                                                CGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGCACGGCATCTCTTTTAAATAC                                               >  SRR4302540.7195893/1‑94 (MQ=60)

                                                 GGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCT                                              >  SRR4302540.7158397/1‑94 (MQ=60)

                                                 GGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCT                                              >  SRR4302540.6090005/1‑94 (MQ=60)

                                                  GTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTG                                             <  SRR4302540.9295996/94‑1 (MQ=60)

                                                  GTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTG                                             <  SRR4302540.8753017/94‑1 (MQ=60)

                                                  GTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTG                                             <  SRR4302540.7110364/94‑1 (MQ=60)

                                                    CCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCT                                           >  SRR4302540.6886579/1‑94 (MQ=60)

                                                    CCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCT                                           >  SRR4302540.4981890/1‑94 (MQ=60)

                                                     CTCTCTCCTTAAACGGTAAACGTATCGCGACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTT                                          <  SRR4302540.8231083/94‑1 (MQ=60)

                                                     CGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTT                                          >  SRR4302540.851994/1‑94 (MQ=60)

                                                     CGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTT                                          >  SRR4302540.4048887/1‑94 (MQ=60)

                                                      GCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTA                                         >  SRR4302540.6211145/1‑94 (MQ=60)

                                                      GCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTA                                         >  SRR4302540.7727210/1‑94 (MQ=60)

                                                      GCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTA                                         >  SRR4302540.10056149/1‑94 (MQ=60)

                                                      GCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTA                                         <  SRR4302540.3093857/94‑1 (MQ=60)

                                                       CTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTAC                                        <  SRR4302540.7734211/94‑1 (MQ=60)

                                                       CTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTAC                                        <  SRR4302540.9732827/94‑1 (MQ=60)

                                                       CTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTAC                                        <  SRR4302540.9652978/94‑1 (MQ=60)

                                                       CTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTAC                                        <  SRR4302540.2669218/94‑1 (MQ=60)

                                                       CTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTAC                                        >  SRR4302540.4820949/1‑94 (MQ=60)

                                                        TCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACT                                       >  SRR4302540.3188483/1‑94 (MQ=60)

                                                        TCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACT                                       >  SRR4302540.2816393/1‑94 (MQ=60)

                                                        TCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACT                                       <  SRR4302540.8626357/94‑1 (MQ=60)

                                                           CCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAA                                    <  SRR4302540.1235887/94‑1 (MQ=60)

                                                           CCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAA                                    >  SRR4302540.6494648/1‑94 (MQ=60)

                                                            CTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAAG                                   >  SRR4302540.3462536/1‑94 (MQ=60)

                                                            CTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAAC                                   <  SRR4302540.4359534/94‑1 (MQ=60)

                                                            CTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAAC                                   <  SRR4302540.5221686/94‑1 (MQ=60)

                                                             TTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACG                                  >  SRR4302540.2280747/1‑94 (MQ=60)

                                                              TAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGG                                 >  SRR4302540.391406/1‑94 (MQ=60)

                                                               AAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGT                                <  SRR4302540.5092230/94‑1 (MQ=60)

                                                                  CGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCT                             <  SRR4302540.5205837/94‑1 (MQ=60)

                                                                  AGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCT                             <  SRR4302540.1558992/94‑1 (MQ=60)

                                                                   GGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTG                            <  SRR4302540.10135553/94‑1 (MQ=60)

                                                                    GTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGATCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGT                           <  SRR4302540.6505769/94‑1 (MQ=60)

                                                                        ACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAA                       <  SRR4302540.7333957/94‑1 (MQ=60)

                                                                        ACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAA                       <  SRR4302540.7043296/94‑1 (MQ=60)

                                                                          GTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGT                     >  SRR4302540.4755200/1‑94 (MQ=60)

                                                                            ATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCG                   >  SRR4302540.9713242/1‑94 (MQ=60)

                                                                            ATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCG                   >  SRR4302540.394477/1‑94 (MQ=60)

                                                                               GCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCC                >  SRR4302540.4993925/1‑94 (MQ=60)

                                                                               GCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCC                >  SRR4302540.5472710/1‑94 (MQ=60)

                                                                                CCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCC               >  SRR4302540.4723122/1‑94 (MQ=60)

                                                                                CCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCC               >  SRR4302540.6331592/1‑94 (MQ=60)

                                                                                  ACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGC             >  SRR4302540.8223585/1‑94 (MQ=60)

                                                                                  ACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGC             >  SRR4302540.9849186/1‑94 (MQ=60)

                                                                                   CCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCG            >  SRR4302540.8598242/1‑94 (MQ=60)

                                                                                   CCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCG            >  SRR4302540.8598243/1‑94 (MQ=60)

                                                                                    CTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGT           <  SRR4302540.8114996/94‑1 (MQ=60)

                                                                                     TCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTG          >  SRR4302540.7823620/1‑94 (MQ=60)

                                                                                       TTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCC        <  SRR4302540.5633838/94‑1 (MQ=60)

                                                                                        TATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCG       >  SRR4302540.7372045/1‑94 (MQ=60)

                                                                                         ATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGG      >  SRR4302540.3404489/1‑94 (MQ=60)

                                                                                           CCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGAC    >  SRR4302540.3691474/1‑94 (MQ=60)

                                                                                           CCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGAC    >  SRR4302540.9907118/1‑94 (MQ=60)

                                                                                           CCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGAC    <  SRR4302540.765424/94‑1 (MQ=60)

                                                                                            CCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACT   >  SRR4302540.5043665/1‑94 (MQ=60)

                                                                                            CCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACT   >  SRR4302540.5572186/1‑94 (MQ=60)

                                                                                            CCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACT   <  SRR4302540.264379/94‑1 (MQ=60)

                                                                                            CCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACT   <  SRR4302540.6784318/94‑1 (MQ=60)

                                                                                            CCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACT   <  SRR4302540.1024020/94‑1 (MQ=60)

                                                                                            CCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACT   <  SRR4302540.6784319/94‑1 (MQ=60)

                                                                                             CCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTG  >  SRR4302540.5922354/1‑94 (MQ=60)

CGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTG  >  NC_000913/2088516‑2088702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`