Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,109,166 A→G V278A (GTC→GCC)  rfbD ← dTDP‑6‑deoxy‑L‑mannose‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,109,1660AG100.0% 783.9 / NA 241V278A (GTC→GCC) rfbDdTDP‑6‑deoxy‑L‑mannose‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (130/111);  total (130/111)

Only 100 of 255 total aligned reads displayed.

TCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACT  >  NC_000913/2109076‑2109255
                                                                                          |                                                                                         
tCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCaggcaaggca                                                                                         <  2:1979604/93‑1 (MQ=255)
 cACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAggcaaggcaa                                                                                        <  2:2571655/93‑1 (MQ=255)
 cACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAggcaaggcaa                                                                                        <  1:2270685/93‑1 (MQ=255)
  aCGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGgcaaggcaag                                                                                       <  2:2426580/93‑1 (MQ=255)
  aCGAACAAGATGCAAAAACTATTAAATAGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCGAGGCAAg                                                                                       >  2:3416326/1‑93 (MQ=255)
   cGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAgc                                                                                      <  1:5443289/93‑1 (MQ=255)
    gAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAgcg                                                                                     <  2:558000/93‑1 (MQ=255)
     aaCAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAgcgc                                                                                    >  1:1516973/1‑93 (MQ=255)
      aCAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCa                                                                                   <  1:720057/93‑1 (MQ=255)
       cAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCaa                                                                                  >  2:2095273/1‑93 (MQ=255)
        aaGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCaaa                                                                                 <  1:3356138/93‑1 (MQ=255)
        aaGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCaaa                                                                                 <  2:182632/93‑1 (MQ=255)
         aGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAg                                                                                >  1:2504026/1‑93 (MQ=255)
          gATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGt                                                                               <  2:5766688/93‑1 (MQ=255)
           aTGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGtt                                                                              >  1:256843/1‑93 (MQ=255)
           aTGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGtt                                                                              <  1:827918/93‑1 (MQ=255)
           aTGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGAGCAAAGtt                                                                              >  2:5191708/1‑93 (MQ=255)
            tGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTc                                                                             <  1:1298423/93‑1 (MQ=255)
              cAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTctg                                                                           <  2:1430070/93‑1 (MQ=255)
              cAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTctg                                                                           >  2:3806702/1‑93 (MQ=255)
                aaaaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTctgct                                                                         <  2:3609911/93‑1 (MQ=255)
                aaaaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTctgct                                                                         >  2:4586628/1‑93 (MQ=255)
                 aaaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTctgctg                                                                        <  1:5533379/93‑1 (MQ=255)
                  aaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGa                                                                       >  1:4126482/1‑93 (MQ=255)
                   aCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGaa                                                                      >  2:4526468/1‑93 (MQ=255)
                      aTTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAtt                                                                   >  1:3122996/1‑93 (MQ=255)
                        tAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAtttt                                                                 <  2:5064512/93‑1 (MQ=255)
                         aaaTTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAttttt                                                                <  1:1388260/93‑1 (MQ=255)
                         aaaTTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAttttt                                                                >  1:926098/1‑93 (MQ=255)
                          aaTTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTc                                                               >  1:3627417/1‑93 (MQ=255)
                            ttGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTg                                                             >  2:5904505/1‑93 (MQ=255)
                             tGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGt                                                            >  1:3731510/1‑93 (MQ=255)
                             tGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGt                                                            <  1:4377381/93‑1 (MQ=255)
                             tGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGt                                                            <  2:4843494/93‑1 (MQ=255)
                              gCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTa                                                           >  2:2882261/1‑93 (MQ=255)
                              gCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTa                                                           <  2:5081194/93‑1 (MQ=255)
                                tGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTAtt                                                         >  2:1300147/1‑93 (MQ=255)
                                tGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTAtt                                                         <  1:4400497/93‑1 (MQ=255)
                                tGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTAtt                                                         <  2:5392124/93‑1 (MQ=255)
                                  tAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTaa                                                       <  2:1860563/93‑1 (MQ=255)
                                  tAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTaa                                                       >  2:5100943/1‑93 (MQ=255)
                                  tAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTaa                                                       <  2:5567951/93‑1 (MQ=255)
                                  tAGTCGTAAATAATCCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTaa                                                       <  2:2838569/93‑1 (MQ=255)
                                    gTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAgg                                                     <  2:3281588/93‑1 (MQ=255)
                                    gTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAgg                                                     <  2:1691533/93‑1 (MQ=255)
                                    gTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAgg                                                     >  1:101817/1‑93 (MQ=255)
                                       gTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCga                                                  >  1:3015591/1‑93 (MQ=255)
                                         aaaTAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCgaga                                                >  1:347090/1‑93 (MQ=255)
                                         aaaTAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCgaga                                                <  2:1773747/93‑1 (MQ=255)
                                          aataatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGagag                                               >  2:827771/1‑93 (MQ=255)
                                          aataatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGagag                                               >  1:3968314/1‑93 (MQ=255)
                                          aataatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGagag                                               >  1:593330/1‑93 (MQ=255)
                                           ataatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGt                                              <  1:5042096/93‑1 (MQ=255)
                                            taatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGtt                                             >  2:5713527/1‑93 (MQ=255)
                                            taatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGtt                                             >  1:6248011/1‑93 (MQ=255)
                                             aatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTa                                            <  2:3463449/93‑1 (MQ=255)
                                             aatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTa                                            >  1:3635578/1‑93 (MQ=255)
                                             aatTCATTGAGCATTCGTTCCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTa                                            >  2:4714797/1‑93 (MQ=255)
                                               ttCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAtg                                          >  2:6260609/1‑93 (MQ=255)
                                               ttCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAtg                                          <  1:661205/93‑1 (MQ=255)
                                                tCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAtgt                                         >  1:6212174/1‑93 (MQ=255)
                                                 cATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAtgtg                                        >  1:4610352/1‑93 (MQ=255)
                                                 cATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAtgtg                                        <  1:4250453/93‑1 (MQ=255)
                                                   ttGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGga                                      >  2:5990570/1‑93 (MQ=255)
                                                   ttGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGga                                      <  2:4544343/93‑1 (MQ=255)
                                                   ttGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGga                                      >  2:3186507/1‑93 (MQ=255)
                                                    tGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGgac                                     <  1:501247/93‑1 (MQ=255)
                                                      aGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGgacga                                   <  1:6128096/93‑1 (MQ=255)
                                                       gCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGgacgac                                  <  2:1516973/93‑1 (MQ=255)
                                                       gCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGgacgac                                  >  1:947909/1‑93 (MQ=255)
                                                       gCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGgacgac                                  >  2:1078808/1‑93 (MQ=255)
                                                        cATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGacgacg                                 >  2:162525/1‑93 (MQ=255)
                                                          ttCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAg                               >  1:5276531/1‑93 (MQ=255)
                                                           tCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGc                              >  1:2693129/1‑93 (MQ=255)
                                                            cGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCt                             >  1:1149423/1‑93 (MQ=255)
                                                             gTTTCACGCCAACCTTCCAGTCAGGCAAGGCGAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTg                            >  2:6124404/1‑93 (MQ=255)
                                                             gTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTg                            <  1:558983/93‑1 (MQ=255)
                                                              tttCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTgg                           <  1:1787898/93‑1 (MQ=255)
                                                               ttCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGgt                          <  1:1898044/93‑1 (MQ=255)
                                                                tCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGgtg                         >  1:2904250/1‑93 (MQ=255)
                                                                tCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGgtg                         <  1:3912933/93‑1 (MQ=255)
                                                                  ccGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTa                       <  2:3091757/92‑1 (MQ=255)
                                                                  aCGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTa                       <  1:4185800/93‑1 (MQ=255)
                                                                       aaCCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTGTTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATa                  >  2:1725644/1‑93 (MQ=255)
                                                                       aaCCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATa                  <  2:4955472/93‑1 (MQ=255)
                                                                       aaCCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATa                  <  1:1938826/93‑1 (MQ=255)
                                                                       aaCCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATa                  >  1:444228/1‑93 (MQ=255)
                                                                        aCCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAg                 >  2:2345057/1‑93 (MQ=255)
                                                                        aCCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAg                 <  2:4225891/93‑1 (MQ=255)
                                                                        aCCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAg                 >  1:434542/1‑93 (MQ=255)
                                                                          cTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGc               <  2:4064278/93‑1 (MQ=255)
                                                                           tGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCt              <  1:472125/93‑1 (MQ=255)
                                                                           tGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCt              <  2:3305970/93‑1 (MQ=255)
                                                                               aGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCtgttg          <  2:5274290/93‑1 (MQ=255)
                                                                                gTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCtgttgt         <  2:2504026/93‑1 (MQ=255)
                                                                                gTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCtgttgt         <  1:2354344/93‑1 (MQ=255)
                                                                                gTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCtgttgt         >  1:2173358/1‑93 (MQ=255)
                                                                                    ggcaaggcaaGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGt     <  2:2964066/93‑1 (MQ=255)
                                                                                    ggcaaggcaaGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGt     <  2:2203640/93‑1 (MQ=255)
                                                                                       aaGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACt  >  1:603542/1‑93 (MQ=255)
                                                                                          |                                                                                         
TCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACT  >  NC_000913/2109076‑2109255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCG  >  NC_000913/2109074‑2109258
                                                                                            |                                                                                            
CATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGC                                                                                             >  SRR4302533.2994817/1‑94 (MQ=60)
   CACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAG                                                                                          <  SRR4302533.2284735/94‑1 (MQ=60)
   CACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAG                                                                                          <  SRR4302533.4165783/94‑1 (MQ=60)
    ACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGC                                                                                         <  SRR4302533.2693183/94‑1 (MQ=60)
    ACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGC                                                                                         >  SRR4302533.1463822/1‑94 (MQ=60)
     CGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCG                                                                                        <  SRR4302533.5476083/94‑1 (MQ=60)
      GAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCACCCTGCCAGTCAGGCAAGGCAAGCGC                                                                                       <  SRR4302533.6286728/94‑1 (MQ=60)
      GAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGC                                                                                       >  SRR4302533.6219491/1‑94 (MQ=60)
      GAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGC                                                                                       >  SRR4302533.1526106/1‑94 (MQ=60)
       AACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCA                                                                                      >  SRR4302533.4362136/1‑94 (MQ=60)
       AACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCA                                                                                      >  SRR4302533.2445420/1‑94 (MQ=60)
       AACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCA                                                                                      >  SRR4302533.5584449/1‑94 (MQ=60)
       AACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCA                                                                                      >  SRR4302533.736395/1‑94 (MQ=60)
        ACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAA                                                                                     <  SRR4302533.724304/94‑1 (MQ=60)
        ACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAA                                                                                     >  SRR4302533.4521567/1‑94 (MQ=60)
          AAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAG                                                                                   <  SRR4302533.3376874/94‑1 (MQ=60)
          AAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAG                                                                                   >  SRR4302533.2519465/1‑94 (MQ=60)
           AGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGT                                                                                  <  SRR4302533.692211/94‑1 (MQ=60)
            GATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTT                                                                                 <  SRR4302533.3654359/94‑1 (MQ=60)
            GATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTT                                                                                 >  SRR4302533.258409/1‑94 (MQ=60)
             ATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTC                                                                                <  SRR4302533.832774/94‑1 (MQ=60)
              TGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCT                                                                               <  SRR4302533.1306237/94‑1 (MQ=60)
              TACAAAAACTATTAAATTGCTGTCGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCT                                                                               <  SRR4302533.4439891/94‑1 (MQ=60)
               GCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTG                                                                              >  SRR4302533.1800465/1‑94 (MQ=60)
                CAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGC                                                                             >  SRR4302533.4608877/1‑94 (MQ=60)
                 AAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAACGTTCTGCT                                                                            >  SRR4302533.1138602/1‑94 (MQ=60)
                 AAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCT                                                                            <  SRR4302533.2154135/94‑1 (MQ=60)
                 AAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCT                                                                            >  SRR4302533.2066307/1‑94 (MQ=60)
                 AAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCT                                                                            <  SRR4302533.1432118/94‑1 (MQ=60)
                 AAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCT                                                                            >  SRR4302533.736302/1‑94 (MQ=60)
                   AAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGA                                                                          <  SRR4302533.5566786/94‑1 (MQ=60)
                   AAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGA                                                                          >  SRR4302533.4151532/1‑94 (MQ=60)
                    AACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAA                                                                         <  SRR4302533.3193750/94‑1 (MQ=60)
                       TATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATT                                                                      >  SRR4302533.1922254/1‑94 (MQ=60)
                       TATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATT                                                                      >  SRR4302533.3142196/1‑94 (MQ=60)
                       TATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTGCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATT                                                                      >  SRR4302533.3393540/1‑94 (MQ=60)
                          TAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTT                                                                   >  SRR4302533.931533/1‑94 (MQ=60)
                           AAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTC                                                                  <  SRR4302533.1396619/94‑1 (MQ=60)
                           AAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTC                                                                  >  SRR4302533.3649737/1‑94 (MQ=60)
                            AATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCT                                                                 <  SRR4302533.4128013/94‑1 (MQ=60)
                             ATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTG                                                                >  SRR4302533.399888/1‑94 (MQ=60)
                              TTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGT                                                               >  SRR4302533.3754398/1‑94 (MQ=60)
                              TTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGT                                                               <  SRR4302533.866614/94‑1 (MQ=60)
                               TGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTA                                                              <  SRR4302533.4403808/94‑1 (MQ=60)
                                  TGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTA                                                           >  SRR4302533.5549991/1‑94 (MQ=60)
                                  TGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTA                                                           >  SRR4302533.1324880/1‑94 (MQ=60)
                                  TGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTA                                                           >  SRR4302533.4378878/1‑94 (MQ=60)
                                  TGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTA                                                           <  SRR4302533.4427067/94‑1 (MQ=60)
                                     AGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGG                                                        >  SRR4302533.102448/1‑94 (MQ=60)
                                     AGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGG                                                        >  SRR4302533.2357125/1‑94 (MQ=60)
                                      GTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGC                                                       <  SRR4302533.6320753/94‑1 (MQ=60)
                                      GTCGTAAATAATTCATTGAGCATTCGTTGCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGC                                                       <  SRR4302533.5083578/94‑1 (MQ=60)
                                       TCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCG                                                      <  SRR4302533.3621828/94‑1 (MQ=60)
                                       TCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCG                                                      <  SRR4302533.1224823/94‑1 (MQ=60)
                                       TCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCG                                                      >  SRR4302533.5500684/1‑94 (MQ=60)
                                        CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGA                                                     >  SRR4302533.6090909/1‑94 (MQ=60)
                                        CGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGA                                                     >  SRR4302533.3034034/1‑94 (MQ=60)
                                          TAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGA                                                   >  SRR4302533.349209/1‑94 (MQ=60)
                                          TAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGA                                                   >  SRR4302533.1221635/1‑94 (MQ=60)
                                           AAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAG                                                  >  SRR4302533.596830/1‑94 (MQ=60)
                                           AAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAG                                                  <  SRR4302533.3990664/94‑1 (MQ=60)
                                           AAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAG                                                  >  SRR4302533.3992486/1‑94 (MQ=60)
                                            AATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGT                                                 >  SRR4302533.4925050/1‑94 (MQ=60)
                                             ATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTT                                                <  SRR4302533.5072611/94‑1 (MQ=60)
                                             ATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTT                                                >  SRR4302533.5906901/1‑94 (MQ=60)
                                             ATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTT                                                >  SRR4302533.6285399/1‑94 (MQ=60)
                                              TAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTA                                               >  SRR4302533.3657933/1‑94 (MQ=60)
                                                 TTCTTTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGT                                            <  SRR4302533.3303215/94‑1 (MQ=60)
                                                 TTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGT                                            <  SRR4302533.665106/94‑1 (MQ=60)
                                                 TTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGT                                            >  SRR4302533.6249425/1‑94 (MQ=60)
                                                  TCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTG                                           >  SRR4302533.4638273/1‑94 (MQ=60)
                                                   CATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGG                                          >  SRR4302533.3866926/1‑94 (MQ=60)
                                                   CATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGG                                          <  SRR4302533.4276099/94‑1 (MQ=60)
                                                   CATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGG                                          >  SRR4302533.2749154/1‑94 (MQ=60)
                                                   CATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAGTTTTCTGTATTAAGGCGAGAGTTATGTGG                                          <  SRR4302533.2638901/94‑1 (MQ=60)
                                                      TGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACG                                       <  SRR4302533.504229/94‑1 (MQ=60)
                                                      TGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGCTATGTGGACG                                       >  SRR4302533.945042/1‑94 (MQ=60)
                                                        AGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGAC                                     <  SRR4302533.6165029/94‑1 (MQ=60)
                                                        AGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGAC                                     >  SRR4302533.953480/1‑94 (MQ=60)
                                                         GCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACG                                    >  SRR4302533.2996717/1‑94 (MQ=60)
                                                           ATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAG                                  >  SRR4302533.3013890/1‑94 (MQ=60)
                                                           ATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAG                                  >  SRR4302533.5308164/1‑94 (MQ=60)
                                                           ATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAG                                  <  SRR4302533.3586311/94‑1 (MQ=60)
                                                            TTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGC                                 >  SRR4302533.2709631/1‑94 (MQ=60)
                                                             TCGTTTCACGCCAACCTGCCAGTCAGGTAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCT                                <  SRR4302533.5062855/94‑1 (MQ=60)
                                                             TCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCT                                >  SRR4302533.1156289/1‑94 (MQ=60)
                                                              CGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTG                               <  SRR4302533.5852112/94‑1 (MQ=60)
                                                              CGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTG                               <  SRR4302533.5351461/94‑1 (MQ=60)
                                                              CGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTG                               <  SRR4302533.390844/94‑1 (MQ=60)
                                                               GTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGG                              <  SRR4302533.562304/94‑1 (MQ=60)
                                                                TTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGT                             <  SRR4302533.1798765/94‑1 (MQ=60)
                                                                TTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGT                             <  SRR4302533.6271953/94‑1 (MQ=60)
                                                                 TTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTG                            >  SRR4302533.2922087/1‑94 (MQ=60)
                                                                 TTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTG                            <  SRR4302533.1909633/94‑1 (MQ=60)
                                                                  TCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGT                           <  SRR4302533.3936830/94‑1 (MQ=60)
                                                                   CACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTA                          <  SRR4302533.4254109/94‑1 (MQ=60)
                                                                    ACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAG                         <  SRR4302533.4211145/94‑1 (MQ=60)
                                                                      GCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGA                       <  SRR4302533.5980625/94‑1 (MQ=60)
                                                                      GCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGA                       >  SRR4302533.4670862/1‑94 (MQ=60)
                                                                      GCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGA                       <  SRR4302533.3654190/94‑1 (MQ=60)
                                                                       CCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGAT                      >  SRR4302533.937367/1‑94 (MQ=60)
                                                                        CAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATA                     >  SRR4302533.6037673/1‑94 (MQ=60)
                                                                        CAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATA                     >  SRR4302533.446884/1‑94 (MQ=60)
                                                                         AACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAG                    >  SRR4302533.437132/1‑94 (MQ=60)
                                                                         AACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAG                    <  SRR4302533.1950650/94‑1 (MQ=60)
                                                                          ACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGG                   <  SRR4302533.2300525/94‑1 (MQ=60)
                                                                          ACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGG                   >  SRR4302533.6188319/1‑94 (MQ=60)
                                                                           CCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGT                  >  SRR4302533.4791004/1‑94 (MQ=60)
                                                                            CTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCT                 <  SRR4302533.3097736/94‑1 (MQ=60)
                                                                            CTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCT                 >  SRR4302533.2449506/1‑94 (MQ=60)
                                                                             TGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTG                >  SRR4302533.4411511/1‑94 (MQ=60)
                                                                             TGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTG                >  SRR4302533.4266006/1‑94 (MQ=60)
                                                                             TGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTG                >  SRR4302533.1830546/1‑94 (MQ=60)
                                                                             TGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTG                <  SRR4302533.474947/94‑1 (MQ=60)
                                                                               CCAGTAAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTT              <  SRR4302533.2216643/94‑1 (MQ=60)
                                                                                 AGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGT            >  SRR4302533.2558651/1‑94 (MQ=60)
                                                                                 AGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGT            >  SRR4302533.2186749/1‑94 (MQ=60)
                                                                                  GTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTT           <  SRR4302533.2368903/94‑1 (MQ=60)
                                                                                   TCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGCGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTG          <  SRR4302533.4462263/94‑1 (MQ=60)
                                                                                   TCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTG          <  SRR4302533.4380481/94‑1 (MQ=60)
                                                                                    CAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGG         >  SRR4302533.1590928/1‑94 (MQ=60)
                                                                                        CAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACT     >  SRR4302533.607088/1‑94 (MQ=60)
                                                                                        CAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACT     >  SRR4302533.5280355/1‑94 (MQ=60)
                                                                                           GGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCG  <  SRR4302533.1410905/94‑1 (MQ=60)
                                                                                           GGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCG  <  SRR4302533.3694938/94‑1 (MQ=60)
                                                                                           GGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCG  <  SRR4302533.3204727/94‑1 (MQ=60)
                                                                                            |                                                                                            
CATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCG  >  NC_000913/2109074‑2109258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑