Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,111,589 A→G M254T (ATG→ACG)  galF ← putative a UDP‑gal transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,111,5890AG100.0% 746.7 / NA 231M254T (ATG→ACG) galFputative a UDP‑gal transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (115/116);  total (115/116)

Only 100 of 239 total aligned reads displayed.

CCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGT  >  NC_000913/2111499‑2111678
                                                                                          |                                                                                         
ccTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGtt                                                                                         <  2:2716117/93‑1 (MQ=255)
ccTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGtt                                                                                         <  1:5977179/93‑1 (MQ=255)
 cttcttTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTg                                                                                        >  2:910353/1‑93 (MQ=255)
 cttcttTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTg                                                                                        >  2:855683/1‑93 (MQ=255)
  ttcttTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGc                                                                                       >  2:1474701/1‑93 (MQ=255)
   tcttTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCa                                                                                      <  2:1737139/93‑1 (MQ=255)
    cttTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCAt                                                                                     >  2:466059/1‑93 (MQ=255)
     tttCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCCGTCGTAACTGTCGCCGGTCATCAGCGTTGCATc                                                                                    >  1:4142806/1‑93 (MQ=255)
     tttCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATc                                                                                    <  1:5285253/93‑1 (MQ=255)
     tttCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATc                                                                                    >  2:6105302/1‑93 (MQ=255)
        cAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAAc                                                                                 <  1:1509784/93‑1 (MQ=255)
           gTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGa                                                                              <  1:1050315/93‑1 (MQ=255)
           gTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGa                                                                              <  2:4518137/93‑1 (MQ=255)
           gTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGa                                                                              >  2:6216098/1‑93 (MQ=255)
             tGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGAtt                                                                            <  1:6135337/93‑1 (MQ=255)
              gcgcAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTg                                                                           <  2:5410235/93‑1 (MQ=255)
              gCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTg                                                                           >  2:5648154/1‑93 (MQ=255)
              gCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTg                                                                           >  2:5962840/1‑93 (MQ=255)
               cGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGt                                                                          <  2:3306293/93‑1 (MQ=255)
                gTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGtt                                                                         <  1:1762848/93‑1 (MQ=255)
                 tAGGCCATACTTCACAGACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTACCTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGttt                                                                        <  2:3155686/93‑1 (MQ=255)
                   ggCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGCCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGttttt                                                                      <  1:4086486/93‑1 (MQ=255)
                    gCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGtttttt                                                                     <  2:1802497/93‑1 (MQ=255)
                     ccATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTc                                                                    <  2:164641/93‑1 (MQ=255)
                      cATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCg                                                                   >  1:1798883/1‑93 (MQ=255)
                      cATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCg                                                                   >  2:3641422/1‑93 (MQ=255)
                       aTACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGc                                                                  >  2:149624/1‑93 (MQ=255)
                        tACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGcc                                                                 >  2:3964113/1‑93 (MQ=255)
                        tACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGcc                                                                 >  2:3557272/1‑93 (MQ=255)
                         tcTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCa                                                                <  1:961124/92‑1 (MQ=255)
                         aCTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCa                                                                <  1:5740324/93‑1 (MQ=255)
                         aCTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCa                                                                <  1:3471574/93‑1 (MQ=255)
                           ttCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCCACGGATTGTTTTTTCGCGAGc                                                              >  2:4201269/1‑93 (MQ=255)
                           ttCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGc                                                              >  1:2704243/1‑93 (MQ=255)
                            tCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCt                                                             >  1:870535/1‑93 (MQ=255)
                             cacaAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTc                                                            <  1:4331770/93‑1 (MQ=255)
                             cacaAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTc                                                            <  1:3004659/93‑1 (MQ=255)
                              acaAACGCCTGCATATAGCTCATTTTTTTGCTGCAGTCGAAACTGTCGCCGGTCATCAGCGTTGCATCGACGGATTGTTTTTTCGCGAGCtag                                                           >  2:2006918/1‑91 (MQ=255)
                              acaAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCg                                                           >  2:3788533/1‑93 (MQ=255)
                              acaAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCg                                                           <  2:1210963/93‑1 (MQ=255)
                              acaAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCg                                                           >  1:2321005/1‑93 (MQ=255)
                              acaAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCg                                                           >  1:2893284/1‑93 (MQ=255)
                               caAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCgg                                                          >  2:664158/1‑93 (MQ=255)
                                aaaCGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGc                                                         >  1:3772296/1‑93 (MQ=255)
                                   cGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAAt                                                      <  1:3646397/93‑1 (MQ=255)
                                     ccTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAg                                                    <  2:2929015/93‑1 (MQ=255)
                                       tGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCa                                                  <  1:3338302/93‑1 (MQ=255)
                                       tGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCACCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCa                                                  <  2:4924194/93‑1 (MQ=255)
                                        gCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAt                                                 >  1:1208998/1‑93 (MQ=255)
                                        gCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAt                                                 >  2:5765020/1‑93 (MQ=255)
                                          atatAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtca                                               <  2:4709839/93‑1 (MQ=255)
                                          atatAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtca                                               >  1:2530936/1‑93 (MQ=255)
                                          atatAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtca                                               <  2:2464717/93‑1 (MQ=255)
                                           tataGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtcag                                              <  1:4923108/93‑1 (MQ=255)
                                            ataGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtcagt                                             <  2:5353888/93‑1 (MQ=255)
                                             taGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtcagtc                                            <  2:2828220/93‑1 (MQ=255)
                                              aGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGTATTGTTTTTTCGCCAGCTCGGCAATAGCAtcagtca                                           <  2:5267254/93‑1 (MQ=255)
                                              aGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtcagtca                                           <  1:472056/93‑1 (MQ=255)
                                                 ccATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGCCAATAGCATCAGTCAGCt                                        >  1:5655505/1‑93 (MQ=255)
                                                  cATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTg                                       >  1:3355650/1‑93 (MQ=255)
                                                  cATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTg                                       >  2:3232681/1‑93 (MQ=255)
                                                   aTTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGa                                      <  1:2557331/93‑1 (MQ=255)
                                                    tttttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGaa                                     <  1:5068729/93‑1 (MQ=255)
                                                         ttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGt                                >  2:766085/1‑93 (MQ=255)
                                                           gCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTcc                              >  2:1938070/1‑93 (MQ=255)
                                                            ccGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTccc                             <  1:4225226/93‑1 (MQ=255)
                                                            ccGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTccc                             <  2:2433798/93‑1 (MQ=255)
                                                             cGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTcccc                            <  2:441019/93‑1 (MQ=255)
                                                              gCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCa                           <  1:5189934/93‑1 (MQ=255)
                                                              gCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCa                           >  2:1888368/1‑93 (MQ=255)
                                                              gCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCa                           <  2:2949570/93‑1 (MQ=255)
                                                               cAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCac                          >  1:2205267/1‑92 (MQ=255)
                                                                 gTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGc                        >  1:2754164/1‑93 (MQ=255)
                                                                  tCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGcc                       >  2:3474832/1‑92 (MQ=255)
                                                                   cGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCAc                      <  1:3278828/93‑1 (MQ=255)
                                                                     tAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCa                    <  1:4905051/93‑1 (MQ=255)
                                                                      aaCTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAg                   <  2:4321233/93‑1 (MQ=255)
                                                                      aaCTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAg                   <  1:5417268/93‑1 (MQ=255)
                                                                       aCTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAgg                  >  2:5074817/1‑93 (MQ=255)
                                                                        cTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGc                 >  2:3916310/1‑93 (MQ=255)
                                                                          gTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTg               >  2:625446/1‑93 (MQ=255)
                                                                           tCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGa              >  2:2237694/1‑93 (MQ=255)
                                                                            cGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAg             <  2:5884480/93‑1 (MQ=255)
                                                                             gCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGt            >  2:1521453/1‑93 (MQ=255)
                                                                             gCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGt            <  2:4285601/93‑1 (MQ=255)
                                                                             ctcGGTCATCAGCGTTGCATCAGCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGt            <  2:4064959/91‑1 (MQ=255)
                                                                               cGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTAc          <  1:2268457/93‑1 (MQ=255)
                                                                                ggTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCCCGCACCAGGCAGAGTGCg         >  2:1612953/1‑93 (MQ=255)
                                                                                ggTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACg         >  1:5784531/1‑93 (MQ=255)
                                                                                ggTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACg         >  2:728350/1‑93 (MQ=255)
                                                                                 gTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGt        >  2:2592757/1‑93 (MQ=255)
                                                                                   catcaGCGTTGCATCAACGGATTGTTTTTGCGCCAGCCCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCTCCAGGCTGAGTACGTTc      <  1:6206168/93‑1 (MQ=255)
                                                                                    ctcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc     <  2:4346058/92‑1 (MQ=255)
                                                                                    atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc     <  2:104125/93‑1 (MQ=255)
                                                                                    atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCAAGCCCCAGGCTGAGTCCGTTcc     >  1:1739599/1‑93 (MQ=255)
                                                                                     tcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCa    >  1:1855136/1‑93 (MQ=255)
                                                                                      caGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAg   <  1:756883/93‑1 (MQ=255)
                                                                                      caGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAg   <  1:1953609/93‑1 (MQ=255)
                                                                                       aGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGt  <  1:4520349/93‑1 (MQ=255)
                                                                                       aGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGt  >  1:6277520/1‑93 (MQ=255)
                                                                                          |                                                                                         
CCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGT  >  NC_000913/2111499‑2111678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCG  >  NC_000913/2111496‑2111682
                                                                                             |                                                                                             
GCCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCG                                                                                               >  SRR4302533.1444474/1‑94 (MQ=60)
GCCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCG                                                                                               <  SRR4302533.4676915/94‑1 (MQ=60)
 CCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGT                                                                                              >  SRR4302533.2529008/1‑94 (MQ=60)
 CCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGT                                                                                              >  SRR4302533.6018297/1‑94 (MQ=60)
 CCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGT                                                                                              >  SRR4302533.5510820/1‑94 (MQ=60)
 CCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGT                                                                                              >  SRR4302533.3995636/1‑94 (MQ=60)
  CCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTT                                                                                             <  SRR4302533.4217310/94‑1 (MQ=60)
   CCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTG                                                                                            <  SRR4302533.6013383/94‑1 (MQ=60)
   CCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTG                                                                                            >  SRR4302533.4347260/1‑94 (MQ=60)
     TTCTTTCAGGTTGCGTAGGCCATACTTCACAAGCGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCA                                                                                          <  SRR4302533.2754015/94‑1 (MQ=60)
      TCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCAT                                                                                         <  SRR4302533.3488422/94‑1 (MQ=60)
       CTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCCGTCGTAACTGTCGCCGGTCATCAGCGTTGCATC                                                                                        >  SRR4302533.4167923/1‑94 (MQ=60)
       CTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATC                                                                                        >  SRR4302533.4827472/1‑94 (MQ=60)
        TTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCA                                                                                       <  SRR4302533.2680900/94‑1 (MQ=60)
        TTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCA                                                                                       <  SRR4302533.5316912/94‑1 (MQ=60)
          TCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAAC                                                                                     >  SRR4302533.4987168/1‑94 (MQ=60)
          TCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAAC                                                                                     >  SRR4302533.6045947/1‑94 (MQ=60)
           CAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACG                                                                                    <  SRR4302533.1518885/94‑1 (MQ=60)
           CAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACG                                                                                    >  SRR4302533.688017/1‑94 (MQ=60)
            AGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGG                                                                                   <  SRR4302533.5718795/94‑1 (MQ=60)
              GTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGAT                                                                                 <  SRR4302533.1056489/94‑1 (MQ=60)
              GTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGAT                                                                                 <  SRR4302533.5331979/94‑1 (MQ=60)
               TTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATT                                                                                <  SRR4302533.2076467/94‑1 (MQ=60)
                TGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTG                                                                               <  SRR4302533.6172304/94‑1 (MQ=60)
                  CGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTT                                                                             >  SRR4302533.4273094/1‑94 (MQ=60)
                  CGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTT                                                                             >  SRR4302533.1799246/1‑94 (MQ=60)
                   GTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTT                                                                            >  SRR4302533.4925097/1‑94 (MQ=60)
                   GTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTT                                                                            <  SRR4302533.1773586/94‑1 (MQ=60)
                    TAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTT                                                                           <  SRR4302533.131581/94‑1 (MQ=60)
                      GGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTT                                                                         >  SRR4302533.3638817/1‑94 (MQ=60)
                      GGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGCCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTT                                                                         <  SRR4302533.4111364/94‑1 (MQ=60)
                       GCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTC                                                                        <  SRR4302533.2653293/94‑1 (MQ=60)
                        CCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCG                                                                       >  SRR4302533.1809829/1‑94 (MQ=60)
                         CATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGC                                                                      >  SRR4302533.3507709/1‑94 (MQ=60)
                            TCTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAG                                                                   <  SRR4302533.966770/94‑1 (MQ=60)
                            ACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAG                                                                   <  SRR4302533.5011221/94‑1 (MQ=60)
                            ACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAG                                                                   <  SRR4302533.5774963/94‑1 (MQ=60)
                            ACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAG                                                                   <  SRR4302533.3493000/94‑1 (MQ=60)
                             CTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGC                                                                  >  SRR4302533.2720831/1‑94 (MQ=60)
                             CTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGC                                                                  >  SRR4302533.5700142/1‑94 (MQ=60)
                             CTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGC                                                                  >  SRR4302533.3369674/1‑94 (MQ=60)
                              TTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCT                                                                 >  SRR4302533.875644/1‑94 (MQ=60)
                                CACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCG                                                               <  SRR4302533.5223251/94‑1 (MQ=60)
                                CACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCG                                                               <  SRR4302533.4357868/94‑1 (MQ=60)
                                CACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCG                                                               <  SRR4302533.3023039/94‑1 (MQ=60)
                                CACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCG                                                               >  SRR4302533.2335371/1‑94 (MQ=60)
                                CACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCG                                                               >  SRR4302533.2911066/1‑94 (MQ=60)
                                  CAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGC                                                             <  SRR4302533.1830271/94‑1 (MQ=60)
                                  CAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGC                                                             >  SRR4302533.3795419/1‑94 (MQ=60)
                                   AAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCA                                                            >  SRR4302533.944377/1‑94 (MQ=60)
                                      CGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATA                                                         <  SRR4302533.3668818/94‑1 (MQ=60)
                                          TGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAT                                                     <  SRR4302533.3232847/94‑1 (MQ=60)
                                          TGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAT                                                     <  SRR4302533.3358936/94‑1 (MQ=60)
                                          TGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAT                                                     >  SRR4302533.1216259/1‑94 (MQ=60)
                                          TGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAT                                                     <  SRR4302533.5368503/94‑1 (MQ=60)
                                            CATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCA                                                   >  SRR4302533.2259929/1‑94 (MQ=60)
                                            CATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCA                                                   >  SRR4302533.2546518/1‑94 (MQ=60)
                                             ATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCAGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAG                                                  >  SRR4302533.5204885/1‑94 (MQ=60)
                                              TATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGT                                                 >  SRR4302533.6248751/1‑94 (MQ=60)
                                              TATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGT                                                 <  SRR4302533.4952933/94‑1 (MQ=60)
                                               ATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTC                                                >  SRR4302533.332919/1‑94 (MQ=60)
                                                TAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCA                                               >  SRR4302533.1519959/1‑94 (MQ=60)
                                                TAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCA                                               <  SRR4302533.4022599/94‑1 (MQ=60)
                                                 AGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAG                                              <  SRR4302533.4120934/94‑1 (MQ=60)
                                                 AGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAG                                              <  SRR4302533.1830348/94‑1 (MQ=60)
                                                 AGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAG                                              <  SRR4302533.474878/94‑1 (MQ=60)
                                                 AGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAG                                              >  SRR4302533.4732671/1‑94 (MQ=60)
                                                  GCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGC                                             <  SRR4302533.2520287/94‑1 (MQ=60)
                                                   CCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCT                                            <  SRR4302533.175555/94‑1 (MQ=60)
                                                   CCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGCCAATAGCATCAGTCAGCT                                            >  SRR4302533.5689681/1‑94 (MQ=60)
                                                    CCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTG                                           >  SRR4302533.3376385/1‑94 (MQ=60)
                                                    CCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTG                                           >  SRR4302533.3013740/1‑94 (MQ=60)
                                                    CCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTG                                           >  SRR4302533.5780466/1‑94 (MQ=60)
                                                    CCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAATCAGCTG                                           <  SRR4302533.2818794/94‑1 (MQ=60)
                                                     CATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGA                                          <  SRR4302533.2567042/94‑1 (MQ=60)
                                                      ATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAA                                         <  SRR4302533.2573056/94‑1 (MQ=60)
                                                       TTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAAT                                        <  SRR4302533.5099335/94‑1 (MQ=60)
                                                            TTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTC                                   <  SRR4302533.3731108/94‑1 (MQ=60)
                                                               CCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCC                                <  SRR4302533.2318130/94‑1 (MQ=60)
                                                               CCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCC                                <  SRR4302533.4250772/94‑1 (MQ=60)
                                                                 GCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCAC                              >  SRR4302533.2218866/1‑94 (MQ=60)
                                                                 GCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCAC                              <  SRR4302533.5221249/94‑1 (MQ=60)
                                                                   AGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGC                            >  SRR4302533.191971/1‑94 (MQ=60)
                                                                   AGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGC                            >  SRR4302533.2771055/1‑94 (MQ=60)
                                                                      CGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACC                         <  SRR4302533.3299073/94‑1 (MQ=60)
                                                                       GTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTATCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCA                        >  SRR4302533.4467072/1‑94 (MQ=60)
                                                                        TAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAG                       <  SRR4302533.4934774/94‑1 (MQ=60)
                                                                        TAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAG                       <  SRR4302533.4934773/94‑1 (MQ=60)
                                                                         AACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGG                      >  SRR4302533.1608394/1‑94 (MQ=60)
                                                                         AACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGG                      >  SRR4302533.6200161/1‑94 (MQ=60)
                                                                         AACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGG                      <  SRR4302533.5449857/94‑1 (MQ=60)
                                                                         AACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGG                      <  SRR4302533.3306202/94‑1 (MQ=60)
                                                                          ACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCcagc                     >  SRR4302533.1119722/1‑90 (MQ=60)
                                                                           CTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCT                    >  SRR4302533.2010470/1‑94 (MQ=60)
                                                                           CTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCT                    >  SRR4302533.5927582/1‑94 (MQ=60)
                                                                            TGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCGGCTGAATACGTCCCCACGCACCAGGCTG                   >  SRR4302533.962820/1‑94 (MQ=60)
                                                                              TCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAG                 <  SRR4302533.68771/94‑1 (MQ=60)
                                                                               CGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGT                <  SRR4302533.6136298/94‑1 (MQ=60)
                                                                                 CCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTAC              <  SRR4302533.5641141/94‑1 (MQ=60)
                                                                                  CGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACG             >  SRR4302533.5819463/1‑94 (MQ=60)
                                                                                  CGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACG             <  SRR4302533.2282494/94‑1 (MQ=60)
                                                                                  CGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACG             >  SRR4302533.4235257/1‑94 (MQ=60)
                                                                                   GGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGT            >  SRR4302533.832545/1‑94 (MQ=60)
                                                                                     TCATCAGCGTTGCATCAACGGATTGTTCTTCCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTC          <  SRR4302533.2344200/94‑1 (MQ=60)
                                                                                      CATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCC         >  SRR4302533.297930/1‑94 (MQ=60)
                                                                                      CATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCAAGCCCCAGGCTGAGTCCGTTCC         >  SRR4302533.1750148/1‑94 (MQ=60)
                                                                                      CATCAGCGTTGCATCAACGGATTGTTTTTGCGCCAGCCCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCTCCAGGCTGAGTACGTTCC         <  SRR4302533.6243388/94‑1 (MQ=60)
                                                                                       ATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCA        <  SRR4302533.4115920/94‑1 (MQ=60)
                                                                                       ATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCA        >  SRR4302533.1866449/1‑94 (MQ=60)
                                                                                         CAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGT      <  SRR4302533.1965515/94‑1 (MQ=60)
                                                                                         CAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGT      <  SRR4302533.761337/94‑1 (MQ=60)
                                                                                         CAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGT      >  SRR4302533.6315001/1‑94 (MQ=60)
                                                                                          AGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTT     >  SRR4302533.1021415/1‑94 (MQ=60)
                                                                                          AGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTT     <  SRR4302533.4547722/94‑1 (MQ=60)
                                                                                           GCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTC    <  SRR4302533.1394643/94‑1 (MQ=60)
                                                                                            CGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCC   >  SRR4302533.3971977/1‑94 (MQ=60)
                                                                                             GTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCG  >  SRR4302533.653818/1‑94 (MQ=60)
                                                                                             GTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCG  <  SRR4302533.642852/94‑1 (MQ=60)
                                                                                             GTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCG  <  SRR4302533.2409916/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCG  >  NC_000913/2111496‑2111682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑