Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,115,180 T→C Q416R (CAG→CGG)  wcaK ← putative galactokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,115,1800TC100.0% 638.2 / NA 197Q416R (CAG→CGG) wcaKputative galactokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (124/73);  total (124/73)

Only 100 of 201 total aligned reads displayed.

GTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCA  >  NC_000913/2115090‑2115269
                                                                                          |                                                                                         
gTTAAGGACGAGGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGc                                                                                         <  2:5341242/93‑1 (MQ=255)
gTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGc                                                                                         >  2:2728042/1‑93 (MQ=255)
 ttAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCa                                                                                        <  2:1540071/93‑1 (MQ=255)
 ttAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCa                                                                                        >  1:2022105/1‑93 (MQ=255)
 ttAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCa                                                                                        <  1:3076491/93‑1 (MQ=255)
  tAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCAc                                                                                       >  2:1511937/1‑93 (MQ=255)
   aaGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCAcc                                                                                      <  2:3826124/93‑1 (MQ=255)
     ggACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCAt                                                                                    <  2:5005218/93‑1 (MQ=255)
     ggACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCAt                                                                                    <  1:4284548/93‑1 (MQ=255)
      gACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATc                                                                                   <  2:185964/93‑1 (MQ=255)
      gACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATc                                                                                   <  1:288171/93‑1 (MQ=255)
       aCGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCt                                                                                  >  1:3328448/1‑93 (MQ=255)
         gAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGc                                                                                >  1:4919794/1‑93 (MQ=255)
          aaGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCa                                                                               <  2:3431174/93‑1 (MQ=255)
             gTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTc                                                                            >  1:5598708/1‑93 (MQ=255)
              tttCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTcc                                                                           <  2:3087424/93‑1 (MQ=255)
              tttCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTcc                                                                           <  1:1935020/93‑1 (MQ=255)
                tCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTg                                                                         <  2:4845845/93‑1 (MQ=255)
                  tGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTc                                                                       >  1:5747953/1‑93 (MQ=255)
                  tGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTc                                                                       <  1:370193/93‑1 (MQ=255)
                   gacgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCt                                                                      >  1:2411541/1‑93 (MQ=255)
                   gacgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCt                                                                      <  1:4467784/93‑1 (MQ=255)
                    acgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTg                                                                     <  1:1405140/93‑1 (MQ=255)
                      gacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGAc                                                                   >  2:4873997/1‑93 (MQ=255)
                      gacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGAc                                                                   >  1:1227507/1‑93 (MQ=255)
                      gacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGAc                                                                   >  1:39534/1‑93 (MQ=255)
                      gacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGAc                                                                   >  1:4182704/1‑93 (MQ=255)
                       acAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACg                                                                  >  2:5202586/1‑93 (MQ=255)
                          gCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTc                                                               <  1:2514764/93‑1 (MQ=255)
                               aaTTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGAc                                                          >  2:3160792/1‑93 (MQ=255)
                                aTTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACt                                                         <  2:5282509/93‑1 (MQ=255)
                                aTTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACt                                                         >  2:820218/1‑93 (MQ=255)
                                 tttCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTg                                                        >  2:4135810/1‑93 (MQ=255)
                                 tttCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTg                                                        >  1:5908393/1‑93 (MQ=255)
                                  ttCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGa                                                       <  2:1585636/93‑1 (MQ=255)
                                  ttCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGa                                                       >  2:6106298/1‑93 (MQ=255)
                                   tCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGAc                                                      <  2:854578/93‑1 (MQ=255)
                                   tCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGAc                                                      <  2:1174506/93‑1 (MQ=255)
                                   tCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGAc                                                      <  1:6048851/93‑1 (MQ=255)
                                      gTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGc                                                   <  1:799686/93‑1 (MQ=255)
                                        aaaaaGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCtt                                                 >  2:597278/1‑93 (MQ=255)
                                        aaaaaGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCtt                                                 >  2:4615689/1‑93 (MQ=255)
                                           aagaagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTNNCGCTCACGACTGACGNCTTNAc                                              >  1:4418566/1‑93 (MQ=255)
                                           aagaagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCAc                                              >  1:4747004/1‑93 (MQ=255)
                                            agaagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACt                                             >  2:1407517/1‑93 (MQ=255)
                                             gaagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTa                                            <  1:827599/93‑1 (MQ=255)
                                             gaagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTa                                            >  2:3983800/1‑93 (MQ=255)
                                              aagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTaa                                           <  1:3415563/93‑1 (MQ=255)
                                               agCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAg                                          >  2:538273/1‑93 (MQ=255)
                                                gCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCCTTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGt                                         >  1:9350/1‑93 (MQ=255)
                                                gCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGt                                         <  2:226480/93‑1 (MQ=255)
                                                gCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGt                                         <  2:3188095/93‑1 (MQ=255)
                                                 ccGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTc                                        <  1:461306/93‑1 (MQ=255)
                                                   gACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcgc                                      <  1:1915915/93‑1 (MQ=255)
                                                    aCCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTTAGTcgcg                                     >  2:262193/1‑93 (MQ=255)
                                                    aCCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcgcg                                     >  2:6075343/1‑93 (MQ=255)
                                                    aCCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcgcg                                     >  2:2069670/1‑93 (MQ=255)
                                                     ccTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTagcgg                                    >  2:5431690/1‑92 (MQ=255)
                                                       ttCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCAt                                  >  2:1478086/1‑93 (MQ=255)
                                                        tCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCAtt                                 <  1:1862026/93‑1 (MQ=255)
                                                           tttCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAg                              >  1:2722859/1‑93 (MQ=255)
                                                           tttCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAg                              >  2:453187/1‑93 (MQ=255)
                                                           tttCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAg                              >  1:580285/1‑93 (MQ=255)
                                                           tttCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAg                              <  1:2867227/93‑1 (MQ=255)
                                                            ttCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgc                             >  2:5726174/1‑93 (MQ=255)
                                                             tCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgcg                            >  2:2429385/1‑93 (MQ=255)
                                                              cACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgcgc                           <  1:5322904/93‑1 (MQ=255)
                                                               aCCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGcc                          >  1:1341585/1‑93 (MQ=255)
                                                               aCCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGcc                          >  1:1652826/1‑93 (MQ=255)
                                                                ccTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCg                         >  2:4003599/1‑93 (MQ=255)
                                                                 cTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCgg                        >  1:182515/1‑93 (MQ=255)
                                                                 cTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCgg                        >  2:1331336/1‑93 (MQ=255)
                                                                  tCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGa                       >  1:4133589/1‑93 (MQ=255)
                                                                   ccccGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGaa                      <  2:1103151/93‑1 (MQ=255)
                                                                   ccccGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGaa                      >  1:3737945/1‑93 (MQ=255)
                                                                     ccGATGCGGTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGc                    >  2:2546388/1‑93 (MQ=255)
                                                                     ccGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGc                    <  1:3769284/93‑1 (MQ=255)
                                                                     ccGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGc                    <  2:442606/93‑1 (MQ=255)
                                                                     ccGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGc                    >  1:6285307/1‑93 (MQ=255)
                                                                      cGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCt                   <  2:3835860/93‑1 (MQ=255)
                                                                       gATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTg                  >  2:881023/1‑93 (MQ=255)
                                                                       gATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTg                  <  2:5250199/93‑1 (MQ=255)
                                                                       gATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTg                  >  2:5538000/1‑93 (MQ=255)
                                                                         tGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGc                >  1:6222304/1‑93 (MQ=255)
                                                                          gcgcTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGcc               <  1:2554760/93‑1 (MQ=255)
                                                                            gcTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTa             >  2:3007453/1‑93 (MQ=255)
                                                                            gcTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTa             >  2:165054/1‑93 (MQ=255)
                                                                             cTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTaa            >  1:5172783/1‑93 (MQ=255)
                                                                             cTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTaa            <  2:2890690/93‑1 (MQ=255)
                                                                               cAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAgg          >  1:3927197/1‑93 (MQ=255)
                                                                                aggCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGt         >  2:6029984/3‑93 (MQ=255)
                                                                                aaGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGt         >  1:2623137/1‑93 (MQ=255)
                                                                                 aGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTa        <  2:2969526/93‑1 (MQ=255)
                                                                                 aGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTa        >  1:365230/1‑93 (MQ=255)
                                                                                   cacaGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATc      <  2:1021650/93‑1 (MQ=255)
                                                                                    acaGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCTCTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATcc     <  1:3383343/93‑1 (MQ=255)
                                                                                      aGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGc   <  1:2265277/93‑1 (MQ=255)
                                                                                      aGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGc   <  2:3318031/93‑1 (MQ=255)
                                                                                       gACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCa  >  1:5362604/1‑93 (MQ=255)
                                                                                       gACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGGATCCGCa  >  2:5070915/1‑93 (MQ=255)
                                                                                          |                                                                                         
GTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCA  >  NC_000913/2115090‑2115269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCA  >  NC_000913/2115087‑2115273
                                                                                             |                                                                                             
TTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACC                                                                                               <  SRR4302533.2975374/94‑1 (MQ=60)
  GGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGC                                                                                             <  SRR4302533.5318749/94‑1 (MQ=60)
   GTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCA                                                                                            >  SRR4302533.2034559/1‑94 (MQ=60)
    TTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCAC                                                                                           <  SRR4302533.3095372/94‑1 (MQ=60)
      AAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCA                                                                                         >  SRR4302533.5082488/1‑94 (MQ=60)
       AGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCAT                                                                                        >  SRR4302533.249082/1‑94 (MQ=60)
       AGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCAT                                                                                        <  SRR4302533.5594064/94‑1 (MQ=60)
        GGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATC                                                                                       <  SRR4302533.4310295/94‑1 (MQ=60)
         GACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCT                                                                                      >  SRR4302533.3349017/1‑94 (MQ=60)
         GACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCT                                                                                      <  SRR4302533.289932/94‑1 (MQ=60)
           CGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGC                                                                                    >  SRR4302533.4949597/1‑94 (MQ=60)
             AAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCAT                                                                                  >  SRR4302533.1848685/1‑94 (MQ=60)
             AAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCAT                                                                                  >  SRR4302533.909416/1‑94 (MQ=60)
             AAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCAT                                                                                  >  SRR4302533.3680005/1‑94 (MQ=60)
              AGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATT                                                                                 <  SRR4302533.3381648/94‑1 (MQ=60)
               GGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTC                                                                                >  SRR4302533.5632533/1‑94 (MQ=60)
                 TTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCT                                                                              <  SRR4302533.1946824/94‑1 (MQ=60)
                  TTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCCTCTGCATTCCTG                                                                             >  SRR4302533.81167/1‑94 (MQ=60)
                   TCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGT                                                                            >  SRR4302533.5374233/1‑94 (MQ=60)
                    CTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTC                                                                           >  SRR4302533.5782629/1‑94 (MQ=60)
                     TGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCT                                                                          >  SRR4302533.2426434/1‑94 (MQ=60)
                     TGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCT                                                                          <  SRR4302533.372428/94‑1 (MQ=60)
                      GACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTG                                                                         <  SRR4302533.4494804/94‑1 (MQ=60)
                       ACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGA                                                                        <  SRR4302533.1413641/94‑1 (MQ=60)
                        CGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGAC                                                                       >  SRR4302533.39796/1‑94 (MQ=60)
                        CGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGAC                                                                       >  SRR4302533.4208035/1‑94 (MQ=60)
                        CGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGAC                                                                       >  SRR4302533.1234857/1‑94 (MQ=60)
                           CAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCT                                                                    <  SRR4302533.4494189/94‑1 (MQ=60)
                           CAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCT                                                                    >  SRR4302533.945592/1‑94 (MQ=60)
                             GCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCA                                                                  <  SRR4302533.2530261/94‑1 (MQ=60)
                               GGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACG                                                                >  SRR4302533.2898253/1‑94 (MQ=60)
                                   ATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTG                                                            >  SRR4302533.5944083/1‑94 (MQ=60)
                                    TTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGA                                                           <  SRR4302533.5666333/94‑1 (MQ=60)
                                     TTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGAC                                                          <  SRR4302533.4424674/94‑1 (MQ=60)
                                     TTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGAC                                                          <  SRR4302533.4136857/94‑1 (MQ=60)
                                      TCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACG                                                         <  SRR4302533.6085497/94‑1 (MQ=60)
                                       CAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGG                                                        >  SRR4302533.630024/1‑94 (MQ=60)
                                        AGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGC                                                       >  SRR4302533.3772398/1‑94 (MQ=60)
                                         GTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCT                                                      >  SRR4302533.1951048/1‑94 (MQ=60)
                                         GTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCT                                                      >  SRR4302533.4189282/1‑94 (MQ=60)
                                         GTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCT                                                      <  SRR4302533.804391/94‑1 (MQ=60)
                                           AAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTC                                                    <  SRR4302533.5302797/94‑1 (MQ=60)
                                           AAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTC                                                    >  SRR4302533.1530177/1‑94 (MQ=60)
                                             AAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTNNCGCTCACGACTGACGNCTTNAC                                                  >  SRR4302533.4445281/1‑94 (MQ=60)
                                             AAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCAC                                                  >  SRR4302533.4775783/1‑94 (MQ=60)
                                                GAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAA                                               <  SRR4302533.832455/94‑1 (MQ=60)
                                                 AAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAG                                              <  SRR4302533.3436668/94‑1 (MQ=60)
                                                  AGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCCTTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGT                                             >  SRR4302533.9430/1‑94 (MQ=60)
                                                    CCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCG                                           <  SRR4302533.464057/94‑1 (MQ=60)
                                                     CGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGC                                          >  SRR4302533.3915476/1‑94 (MQ=60)
                                                      GACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCG                                         <  SRR4302533.1927610/94‑1 (MQ=60)
                                                      GACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCG                                         >  SRR4302533.3752120/1‑94 (MQ=60)
                                                        CCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCA                                       >  SRR4302533.4530144/1‑94 (MQ=60)
                                                        CCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCA                                       >  SRR4302533.5890248/1‑94 (MQ=60)
                                                          GTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATTTGCATTCCTGGCTGACGCTCACGACCGACGGCTTCACTAAGTCGCGCATC                                     >  SRR4302533.6345118/1‑94 (MQ=60)
                                                           TCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTC                                    <  SRR4302533.1873380/94‑1 (MQ=60)
                                                           TCATTGCGCCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACACTCACGACTGACGGCTTCACTAAGTCGCGCATTC                                    <  SRR4302533.6040884/94‑1 (MQ=60)
                                                            CATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCA                                   >  SRR4302533.3588570/1‑94 (MQ=60)
                                                             ATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAG                                  >  SRR4302533.4226648/1‑94 (MQ=60)
                                                             ATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAG                                  >  SRR4302533.1880792/1‑94 (MQ=60)
                                                             ATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAG                                  >  SRR4302533.583700/1‑94 (MQ=60)
                                                             ATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAG                                  >  SRR4302533.318463/1‑94 (MQ=60)
                                                             ATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAG                                  >  SRR4302533.2422123/1‑94 (MQ=60)
                                                             ATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAG                                  >  SRR4302533.2739568/1‑94 (MQ=60)
                                                              TTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGC                                 <  SRR4302533.2884868/94‑1 (MQ=60)
                                                               TTCCCCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCG                                <  SRR4302533.1368618/94‑1 (MQ=60)
                                                               TTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCG                                >  SRR4302533.2869114/1‑94 (MQ=60)
                                                                TCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGC                               <  SRR4302533.4834604/94‑1 (MQ=60)
                                                                 CACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCC                              <  SRR4302533.5354802/94‑1 (MQ=60)
                                                                 CACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCC                              <  SRR4302533.6120419/94‑1 (MQ=60)
                                                                 CACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCC                              >  SRR4302533.1662837/1‑94 (MQ=60)
                                                                 CACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCC                              >  SRR4302533.1349663/1‑94 (MQ=60)
                                                                  ACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCG                             >  SRR4302533.254167/1‑94 (MQ=60)
                                                                   CCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGG                            >  SRR4302533.183650/1‑94 (MQ=60)
                                                                   CCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGG                            >  SRR4302533.4689831/1‑94 (MQ=60)
                                                                    CTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGA                           >  SRR4302533.4158679/1‑94 (MQ=60)
                                                                    CTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGA                           >  SRR4302533.343084/1‑94 (MQ=60)
                                                                     TCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAA                          >  SRR4302533.3760871/1‑94 (MQ=60)
                                                                     TCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAA                          >  SRR4302533.3952953/1‑94 (MQ=60)
                                                                     TCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAA                          >  SRR4302533.1300408/1‑94 (MQ=60)
                                                                      CCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAG                         <  SRR4302533.5810122/94‑1 (MQ=60)
                                                                       CCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGC                        >  SRR4302533.6322810/1‑94 (MQ=60)
                                                                        CCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCT                       <  SRR4302533.3792393/94‑1 (MQ=60)
                                                                           ATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGC                    >  SRR4302533.6259597/1‑94 (MQ=60)
                                                                           ATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGC                    >  SRR4302533.2696124/1‑94 (MQ=60)
                                                                             GCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCT                  <  SRR4302533.1780183/94‑1 (MQ=60)
                                                                             GCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCT                  <  SRR4302533.2570476/94‑1 (MQ=60)
                                                                               GCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAA                >  SRR4302533.5203995/1‑94 (MQ=60)
                                                                                 TCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGG              <  SRR4302533.2586132/94‑1 (MQ=60)
                                                                                 TCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGG              >  SRR4302533.3951172/1‑94 (MQ=60)
                                                                                  CAGGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGT             <  SRR4302533.1933163/94‑1 (MQ=60)
                                                                                  CAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGT             >  SRR4302533.2639237/1‑94 (MQ=60)
                                                                                   AAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTA            >  SRR4302533.2208033/1‑94 (MQ=60)
                                                                                   AAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTA            >  SRR4302533.367443/1‑94 (MQ=60)
                                                                                       ACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCTCTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCG        <  SRR4302533.3404231/94‑1 (MQ=60)
                                                                                       ACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCG        <  SRR4302533.3063432/94‑1 (MQ=60)
                                                                                         AGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCA      <  SRR4302533.2279294/94‑1 (MQ=60)
                                                                                         AGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCA      >  SRR4302533.5394811/1‑94 (MQ=60)
                                                                                          GACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAA     >  SRR4302533.5668497/1‑94 (MQ=60)
                                                                                            CCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACC   >  SRR4302533.2927712/1‑94 (MQ=60)
                                                                                             CGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCA  >  SRR4302533.31091/1‑94 (MQ=60)
                                                                                             CGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCA  >  SRR4302533.337537/1‑94 (MQ=60)
                                                                                             CGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCA  >  SRR4302533.2259797/1‑94 (MQ=60)
                                                                                             CGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCA  >  SRR4302533.2131551/1‑94 (MQ=60)
                                                                                             |                                                                                             
TTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCA  >  NC_000913/2115087‑2115273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑