Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,115,180	T→C	Q416R (CAG→CGG)	wcaK ←	putative galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,115,180	0	T	C	100.0%	711.8 / NA	223	Q416R (CAG→CGG)	wcaK	putative galactokinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (114/109); total (114/109)

Only 100 of 230 total aligned reads displayed.

GTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCA  >  NC_000913/2115090‑2115269

  tAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCAc                                                                                       >  2:6118875/1‑93 (MQ=255)

  tAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCAc                                                                                       >  1:598044/1‑93 (MQ=255)

     ggACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCAt                                                                                    <  2:6815601/93‑1 (MQ=255)

     ggACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCAt                                                                                    >  2:869336/1‑93 (MQ=255)

       aCGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCt                                                                                  >  2:1716729/1‑93 (MQ=255)

       aCGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCt                                                                                  >  1:1923559/1‑93 (MQ=255)

       aCGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCt                                                                                  >  2:2616499/1‑93 (MQ=255)

        cGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTg                                                                                 >  2:9616563/1‑93 (MQ=255)

        cGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTg                                                                                 <  1:5052940/93‑1 (MQ=255)

        cGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTg                                                                                 >  1:529057/1‑93 (MQ=255)

        cGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTg                                                                                 <  1:8196017/93‑1 (MQ=255)

          aaGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCa                                                                               >  1:3341988/1‑93 (MQ=255)

          aaGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCa                                                                               >  1:8718049/1‑93 (MQ=255)

           aGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCAt                                                                              >  2:3185048/1‑93 (MQ=255)

           aGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCAt                                                                              >  2:9121637/1‑93 (MQ=255)

           aGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCAt                                                                              <  1:425318/93‑1 (MQ=255)

            ggTTTCTGACGACAGCGGGACTTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGGTCAAGCACAGACCCCACCATCTGCAtt                                                                             >  2:8221063/1‑93 (MQ=255)

             gTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTc                                                                            >  1:9775824/1‑93 (MQ=255)

              tttCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTcc                                                                           <  2:6481314/93‑1 (MQ=255)

               ttCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCt                                                                          <  2:4606290/93‑1 (MQ=255)

                   gacgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCt                                                                      >  1:2912107/1‑93 (MQ=255)

                    acgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTg                                                                     >  1:8323700/1‑93 (MQ=255)

                     cgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGa                                                                    <  2:7882740/93‑1 (MQ=255)

                     cgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGa                                                                    <  1:9359106/93‑1 (MQ=255)

                     cgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGa                                                                    >  1:1808060/1‑93 (MQ=255)

                     cgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGa                                                                    <  2:572423/93‑1 (MQ=255)

                     cgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGa                                                                    >  1:5581606/1‑93 (MQ=255)

                      gacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGAc                                                                   <  2:3275827/93‑1 (MQ=255)

                       acAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACg                                                                  >  1:5832457/1‑93 (MQ=255)

                        cAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGc                                                                 <  1:4686124/93‑1 (MQ=255)

                        cAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGc                                                                 >  2:4400091/1‑93 (MQ=255)

                         aGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCt                                                                >  2:4754238/1‑93 (MQ=255)

                           cGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCa                                                              >  2:8073499/1‑93 (MQ=255)

                            gggAATTTCAGTAAAAAGAAGCCAACCTTCATTTCACCTCCCCGATGCCCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCAc                                                             <  2:6312536/93‑1 (MQ=255)

                             ggAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACg                                                            >  1:2135882/1‑93 (MQ=255)

                              gAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGa                                                           >  1:3471509/1‑93 (MQ=255)

                              gAAATTCAATAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGa                                                           <  2:1109694/93‑1 (MQ=255)

                               aaTTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGAc                                                          <  1:6239540/93‑1 (MQ=255)

                               aaTTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGAc                                                          <  1:7202965/93‑1 (MQ=255)

                                aTTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACt                                                         >  1:2482136/1‑93 (MQ=255)

                                aTTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACt                                                         >  1:2417562/1‑93 (MQ=255)

                                   tCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGAc                                                      >  1:5277387/1‑93 (MQ=255)

                                   tCAATAAAAAGAAGCCGAACTTCATTTACCATCCAAGAAGCGCTAAAGCACAGACCGCACCATCAGAATTCCTGTCTGACGCTAAagacagac                                                      <  1:8940458/93‑1 (MQ=255)

                                     aGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACgg                                                    >  2:1814782/1‑93 (MQ=255)

                                       tAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCt                                                  >  2:2318083/1‑93 (MQ=255)

                                        aaaaaGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCtt                                                 <  1:7699675/93‑1 (MQ=255)

                                          aaaGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCTCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCa                                               <  1:8105910/93‑1 (MQ=255)

                                           aagaagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCAc                                              <  2:9339825/93‑1 (MQ=255)

                                           aagaagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCAc                                              <  1:4107610/93‑1 (MQ=255)

                                            agaagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACt                                             >  1:7992231/1‑93 (MQ=255)

                                             gaagCCGACCTTCATTTCGCCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTa                                            <  2:5111568/93‑1 (MQ=255)

                                             gaagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTa                                            <  1:8864968/93‑1 (MQ=255)

                                             gaagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTa                                            <  2:8930376/93‑1 (MQ=255)

                                              aagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTaa                                           <  2:8469128/93‑1 (MQ=255)

                                              aagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTaa                                           <  1:9186669/93‑1 (MQ=255)

                                                gCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGt                                         <  1:9526047/93‑1 (MQ=255)

                                                gCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGt                                         >  2:5974495/1‑93 (MQ=255)

                                                gCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGt                                         <  1:7453443/93‑1 (MQ=255)

                                                 ccGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGCCCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTc                                        <  1:4020101/93‑1 (MQ=255)

                                                  cGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcg                                       <  1:653121/93‑1 (MQ=255)

                                                  cGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcg                                       <  2:4223091/93‑1 (MQ=255)

                                                    aCCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcgcg                                     >  1:1627492/1‑93 (MQ=255)

                                                      cTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCa                                   <  1:4421110/93‑1 (MQ=255)

                                                        tCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCAtt                                 <  2:8898614/93‑1 (MQ=255)

                                                         taTTTCACCTCCCCGATGCGCTCAAGCACAGGCCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTc                                <  1:8405059/92‑1 (MQ=255)

                                                          aTTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCa                               >  2:10016228/1‑93 (MQ=255)

                                                          aTTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCa                               <  2:4316448/93‑1 (MQ=255)

                                                           tttCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAg                              <  2:2774181/93‑1 (MQ=255)

                                                           tttCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAg                              <  1:985419/93‑1 (MQ=255)

                                                           tttCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAg                              >  1:3401954/1‑93 (MQ=255)

                                                           tttCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAg                              <  1:4216477/93‑1 (MQ=255)

                                                            ttCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgc                             <  2:4762480/93‑1 (MQ=255)

                                                             tCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgcg                            <  2:7404028/93‑1 (MQ=255)

                                                             tCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgcg                            >  1:461621/1‑93 (MQ=255)

                                                              cACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgcgc                           <  1:1447466/93‑1 (MQ=255)

                                                              cACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgcgc                           >  2:1371361/1‑93 (MQ=255)

                                                                ccTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCg                         >  1:1654447/1‑93 (MQ=255)

                                                                ccTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCg                         >  1:5653569/1‑93 (MQ=255)

                                                                 cTCCCCGATGCGCTCAAGCACAGCCCGCCCCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCgg                        <  1:6864922/93‑1 (MQ=255)

                                                                  cccccGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGa                       <  1:7750603/92‑1 (MQ=255)

                                                                   ccccGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGaa                      <  2:9585091/93‑1 (MQ=255)

                                                                     ccGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGc                    >  1:4990007/1‑93 (MQ=255)

                                                                     ccGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGc                    <  2:1269345/93‑1 (MQ=255)

                                                                     ccGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGc                    >  2:508029/1‑93 (MQ=255)

                                                                       gATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTg                  >  1:7953753/1‑93 (MQ=255)

                                                                        aTGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTgg                 <  1:7319715/93‑1 (MQ=255)

                                                                         tGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGc                <  2:5381380/93‑1 (MQ=255)

                                                                            gcTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGAGCCGGAAGCTGGCCTa             >  1:1034044/1‑93 (MQ=255)

                                                                             cTCAAGCACAGGCCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTaa            <  1:9510051/93‑1 (MQ=255)

                                                                              tCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAg           >  2:5873203/1‑93 (MQ=255)

                                                                              tCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAg           <  1:2530080/93‑1 (MQ=255)

                                                                                aaGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGt         >  2:744628/1‑93 (MQ=255)

                                                                                 aGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTa        <  2:9503372/93‑1 (MQ=255)

                                                                                   cacaGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGTAAGCTGGCCTAAGGTATc      <  2:4727/93‑1 (MQ=255)

                                                                                   cacaGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATc      <  1:8126994/93‑1 (MQ=255)

                                                                                   cacaGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATc      >  2:6422173/1‑93 (MQ=255)

                                                                                    acaGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATcc     >  2:9068583/1‑93 (MQ=255)

                                                                                      aGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGc   <  1:5414381/93‑1 (MQ=255)

                                                                                       gACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCa  >  1:3884118/1‑93 (MQ=255)

                                                                                       gACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGGATCCGCa  >  1:7651044/1‑93 (MQ=255)

GTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCA  >  NC_000913/2115090‑2115269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCA  >  NC_000913/2115087‑2115273

TTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACC                                                                                               >  SRR4302540.9708082/1‑94 (MQ=60)

TTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACC                                                                                               <  SRR4302540.259886/94‑1 (MQ=60)

 TGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCG                                                                                              <  SRR4302540.7656549/94‑1 (MQ=60)

  GGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGC                                                                                             >  SRR4302540.2459787/1‑94 (MQ=60)

    TTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCAC                                                                                           >  SRR4302540.604462/1‑94 (MQ=60)

     TAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACC                                                                                          >  SRR4302540.9107489/1‑94 (MQ=60)

      ATGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCT                                                                                         >  SRR4302540.7163919/1‑94 (MQ=60)

       AGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCAT                                                                                        >  SRR4302540.3463197/1‑94 (MQ=60)

       AGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACATTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCAT                                                                                        >  SRR4302540.594690/1‑94 (MQ=60)

         GACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCT                                                                                      <  SRR4302540.4690752/94‑1 (MQ=60)

         GACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCT                                                                                      <  SRR4302540.3656081/94‑1 (MQ=60)

         GACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCT                                                                                      >  SRR4302540.1944530/1‑94 (MQ=60)

          ACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTG                                                                                     >  SRR4302540.534775/1‑94 (MQ=60)

           CGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGC                                                                                    <  SRR4302540.5107565/94‑1 (MQ=60)

           CGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGC                                                                                    <  SRR4302540.8291261/94‑1 (MQ=60)

            GAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCA                                                                                   >  SRR4302540.8819268/1‑94 (MQ=60)

            GAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCA                                                                                   >  SRR4302540.3378661/1‑94 (MQ=60)

            GAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCA                                                                                   >  SRR4302540.7948159/1‑94 (MQ=60)

             AAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCAT                                                                                  <  SRR4302540.5901641/94‑1 (MQ=60)

             AAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCAT                                                                                  <  SRR4302540.1724759/94‑1 (MQ=60)

              AGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATT                                                                                 <  SRR4302540.429794/94‑1 (MQ=60)

               GGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTC                                                                                >  SRR4302540.9890420/1‑94 (MQ=60)

               GGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGATCAAGCACAGACCGCACCATCTGCATTC                                                                                >  SRR4302540.7916662/1‑94 (MQ=60)

                GTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCC                                                                               >  SRR4302540.1665966/1‑94 (MQ=60)

                 TTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCT                                                                              <  SRR4302540.6965655/94‑1 (MQ=60)

                 TTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCT                                                                              <  SRR4302540.3755569/94‑1 (MQ=60)

                   TCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGT                                                                            <  SRR4302540.7553436/94‑1 (MQ=60)

                   TCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGT                                                                            >  SRR4302540.4900674/1‑94 (MQ=60)

                    CTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTC                                                                           >  SRR4302540.8207394/1‑94 (MQ=60)

                     ngnnnncnanngnaantncannanannGAAGCCGNCCTTAATTTCNCNTNCCCGANGNGCTCANGNANANACCGCACCATCTGCATTCCTGTCT                                                                          <  SRR4302540.9641518/67‑1 (MQ=38)

                     TGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCT                                                                          <  SRR4302540.138975/94‑1 (MQ=60)

                     TGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCT                                                                          >  SRR4302540.2943944/1‑94 (MQ=60)

                      GACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTG                                                                         >  SRR4302540.8420309/1‑94 (MQ=60)

                       ACGACGGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGA                                                                        >  SRR4302540.1827504/1‑94 (MQ=60)

                       ACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGA                                                                        >  SRR4302540.5643047/1‑94 (MQ=60)

                       ACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGA                                                                        >  SRR4302540.1827759/1‑94 (MQ=60)

                        CGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGAC                                                                       <  SRR4302540.9383474/94‑1 (MQ=60)

                        CGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGAC                                                                       <  SRR4302540.8242162/94‑1 (MQ=60)

                        CGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGAC                                                                       <  SRR4302540.9470566/94‑1 (MQ=60)

                        CGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGAC                                                                       >  SRR4302540.4545941/1‑94 (MQ=60)

                         GACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACG                                                                      >  SRR4302540.5896950/1‑94 (MQ=60)

                           CAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCT                                                                    <  SRR4302540.4736244/94‑1 (MQ=60)

                               GGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACG                                                                >  SRR4302540.2158814/1‑94 (MQ=60)

                                GGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGA                                                               >  SRR4302540.3509306/1‑94 (MQ=60)

                                  AATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACT                                                             <  SRR4302540.7283270/94‑1 (MQ=60)

                                  AATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACT                                                             >  SRR4302540.2443613/1‑94 (MQ=60)

                                  AATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACT                                                             <  SRR4302540.6308964/94‑1 (MQ=60)

                                  AATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACT                                                             >  SRR4302540.5425393/1‑94 (MQ=60)

                                  AATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACT                                                             >  SRR4302540.2508877/1‑94 (MQ=60)

                                  AATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACT                                                             >  SRR4302540.3351109/1‑94 (MQ=60)

                                    TTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGA                                                           >  SRR4302540.5122065/1‑94 (MQ=60)

                                    TTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGA                                                           <  SRR4302540.8160255/94‑1 (MQ=60)

                                     TTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGAC                                                          >  SRR4302540.5335011/1‑94 (MQ=60)

                                     TTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGAC                                                          >  SRR4302540.8236966/1‑94 (MQ=60)

                                       CAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGcagg                                                        >  SRR4302540.2619916/1‑90 (MQ=60)

                                        AGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGC                                                       >  SRR4302540.7681799/1‑94 (MQ=60)

                                        AGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGC                                                       >  SRR4302540.8502274/1‑94 (MQ=60)

                                         GTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCT                                                      >  SRR4302540.1877831/1‑94 (MQ=60)

                                           AAAAAGAAGCCGACCTTCATTTCCCCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTC                                                    <  SRR4302540.7787580/94‑1 (MQ=60)

                                           AAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTC                                                    >  SRR4302540.8579217/1‑94 (MQ=60)

                                           AAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTC                                                    <  SRR4302540.7787579/94‑1 (MQ=60)

                                            AAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCA                                                   >  SRR4302540.9792707/1‑94 (MQ=60)

                                            AAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCA                                                   >  SRR4302540.4244437/1‑94 (MQ=60)

                                             AAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCTCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCAC                                                  <  SRR4302540.8199989/94‑1 (MQ=60)

                                             AAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCAC                                                  >  SRR4302540.9632662/1‑94 (MQ=60)

                                             AAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCAC                                                  >  SRR4302540.4374216/1‑94 (MQ=60)

                                             AAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCAC                                                  <  SRR4302540.4049580/94‑1 (MQ=60)

                                              AAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACT                                                 <  SRR4302540.4151927/94‑1 (MQ=60)

                                              AAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACT                                                 >  SRR4302540.8085273/1‑94 (MQ=60)

                                               AGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTA                                                <  SRR4302540.9681454/94‑1 (MQ=60)

                                               AGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGATTCACTA                                                >  SRR4302540.6711514/1‑94 (MQ=60)

                                                GAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAA                                               <  SRR4302540.8967549/94‑1 (MQ=60)

                                                GAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCAGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAA                                               <  SRR4302540.8967550/94‑1 (MQ=60)

                                                 AAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAG                                              <  SRR4302540.9296840/94‑1 (MQ=60)

                                                 AAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAG                                              <  SRR4302540.9921841/94‑1 (MQ=60)

                                                   GCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTC                                            <  SRR4302540.7536537/94‑1 (MQ=60)

                                                   GCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTC                                            <  SRR4302540.2209739/94‑1 (MQ=60)

                                                   GCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTC                                            <  SRR4302540.6143705/94‑1 (MQ=60)

                                                   GCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTC                                            <  SRR4302540.9638837/94‑1 (MQ=60)

                                                    CCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGCCCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCG                                           <  SRR4302540.4063413/94‑1 (MQ=60)

                                                    CCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCG                                           >  SRR4302540.8892986/1‑94 (MQ=60)

                                                     CGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGC                                          <  SRR4302540.660078/94‑1 (MQ=60)

                                                     CGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGC                                          >  SRR4302540.5342691/1‑94 (MQ=60)

                                                      GACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCG                                         >  SRR4302540.1645317/1‑94 (MQ=60)

                                                         CTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCAT                                      <  SRR4302540.4469012/94‑1 (MQ=60)

                                                          TTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATT                                     >  SRR4302540.1573134/1‑94 (MQ=60)

                                                           TCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTC                                    <  SRR4302540.9074676/94‑1 (MQ=60)

                                                            TATTTCACCTCCCCGATGCGCTCAAGCACAGGCCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCA                                   <  SRR4302540.8502617/94‑1 (MQ=60)

                                                             ATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAG                                  >  SRR4302540.3439040/1‑94 (MQ=60)

                                                              TTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGC                                 <  SRR4302540.995493/94‑1 (MQ=60)

                                                              TTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGC                                 <  SRR4302540.4262382/94‑1 (MQ=60)

                                                              TTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGC                                 <  SRR4302540.4262383/94‑1 (MQ=60)

                                                              TTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGC                                 <  SRR4302540.5539448/94‑1 (MQ=60)

                                                               TTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCG                                >  SRR4302540.466547/1‑94 (MQ=60)

                                                                TCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGC                               >  SRR4302540.877249/1‑94 (MQ=60)

                                                                 CACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCC                              <  SRR4302540.1463204/94‑1 (MQ=60)

                                                                  ACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCG                             >  SRR4302540.5715814/1‑94 (MQ=60)

                                                                  ACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCG                             >  SRR4302540.5715815/1‑94 (MQ=60)

                                                                  ACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCG                             >  SRR4302540.1672475/1‑94 (MQ=60)

                                                                   CCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGG                            >  SRR4302540.2340424/1‑94 (MQ=60)

                                                                    CTCCCCGATGCGCTCAAGCACAGCCCGCCCCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGA                           <  SRR4302540.6941004/94‑1 (MQ=60)

                                                                     CCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAA                          <  SRR4302540.7841120/94‑1 (MQ=60)

                                                                      CCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAG                         >  SRR4302540.4202480/1‑94 (MQ=60)

                                                                      CCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAG                         <  SRR4302540.8380152/94‑1 (MQ=60)

                                                                       CCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGC                        >  SRR4302540.5044035/1‑94 (MQ=60)

                                                                       CCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGC                        >  SRR4302540.5044036/1‑94 (MQ=60)

                                                                       CCCGATGCGCTAAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGC                        <  SRR4302540.1987019/94‑1 (MQ=60)

                                                                        CCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCT                       <  SRR4302540.653750/94‑1 (MQ=60)

                                                                        CCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCT                       >  SRR4302540.4116861/1‑94 (MQ=60)

                                                                         CGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTG                      >  SRR4302540.8046465/1‑94 (MQ=60)

                                                                          GATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGG                     >  SRR4302540.5180254/1‑94 (MQ=60)

                                                                           ATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGC                    <  SRR4302540.7401465/94‑1 (MQ=60)

                                                                           ATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGC                    <  SRR4302540.7401466/94‑1 (MQ=60)

                                                                              CGCTCAAGCACAGACCGCCCTGTGTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTA                 <  SRR4302540.2936989/94‑1 (MQ=60)

                                                                              CGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGAGCCGGAAGCTGGCCTA                 >  SRR4302540.1044557/1‑94 (MQ=60)

                                                                                CTCAAGCACAGGCCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAG               <  SRR4302540.9622704/94‑1 (MQ=60)

                                                                                CTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAG               >  SRR4302540.8051558/1‑94 (MQ=60)

                                                                                CTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAG               <  SRR4302540.5705681/94‑1 (MQ=60)

                                                                                 TCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGG              <  SRR4302540.2557381/94‑1 (MQ=60)

                                                                                   AAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCCGCGCCGGAAGCTGGCCTAAGGTA            >  SRR4302540.2529877/1‑94 (MQ=60)

                                                                                   AAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTA            >  SRR4302540.9595804/1‑94 (MQ=60)

                                                                                    AGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTAT           >  SRR4302540.7745083/1‑94 (MQ=60)

                                                                                    AGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTAT           >  SRR4302540.3412953/1‑94 (MQ=60)

                                                                                      CACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCC         <  SRR4302540.1009464/94‑1 (MQ=60)

                                                                                      CACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCC         >  SRR4302540.7514316/1‑94 (MQ=60)

                                                                                      CACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCC         <  SRR4302540.8221322/94‑1 (MQ=60)

                                                                                         AGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCA      <  SRR4302540.5473915/94‑1 (MQ=60)

                                                                                         AGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCA      <  SRR4302540.5473914/94‑1 (MQ=60)

                                                                                         AGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCA      >  SRR4302540.3926314/1‑94 (MQ=60)

                                                                                         AGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGGATCCGCA      >  SRR4302540.7737176/1‑94 (MQ=60)

                                                                                          GACCGCACTATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCGCTAAGTCGCGCATACAGCGCCGGAAGCTGGCCTAAGGTATCCGCAA     <  SRR4302540.4502723/94‑1 (MQ=60)

                                                                                            CCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACC   <  SRR4302540.2658453/94‑1 (MQ=60)

                                                                                            CCGCACCATCTGCATTCCGGTCTGACGCTCCCGACTGACGGCTTCACTAAGTCGCGCATGCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACC   <  SRR4302540.8398529/94‑1 (MQ=60)

                                                                                             CGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCA  >  SRR4302540.7838746/1‑94 (MQ=60)

                                                                                             CGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCA  >  SRR4302540.123981/1‑94 (MQ=60)

TTGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCA  >  NC_000913/2115087‑2115273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`