Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,331,416 T→C intergenic (‑3599/+940) b2226 ← / ← b2229 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,331,4160TC99.5% 650.6 / NA 203intergenic (‑3599/+940)b2226/b2229hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (90/112);  minor base G (1/0);  total (91/112)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

CCGATCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGTAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTAACTGCAAG  >  NC_000913/2331326‑2331505
                                                                                          |                                                                                         
ccGATCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCag                                                                                         <  2:6121594/93‑1 (MQ=255)
 cGATCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCaga                                                                                        >  2:501694/1‑93 (MQ=255)
 cGATCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCaga                                                                                        >  1:2734360/1‑93 (MQ=255)
 cGATCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCaga                                                                                        <  2:1606577/93‑1 (MQ=255)
  gATCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGGagaa                                                                                       >  1:5403166/1‑93 (MQ=255)
  gATCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCagaa                                                                                       >  1:1322295/1‑93 (MQ=255)
  gATCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCagaa                                                                                       >  1:3484287/1‑93 (MQ=255)
    tCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCagaaga                                                                                     <  1:2991079/93‑1 (MQ=255)
    tCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCagaaga                                                                                     <  2:1947761/93‑1 (MQ=255)
     cACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAgaagaa                                                                                    <  1:3895424/93‑1 (MQ=255)
          taccATCGACACGATCCCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTtct                                                                               <  2:216101/90‑1 (MQ=255)
          tAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTtct                                                                               >  1:6092222/1‑93 (MQ=255)
          tAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTtct                                                                               <  2:4117307/93‑1 (MQ=255)
           aaCATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTtctc                                                                              <  1:424605/93‑1 (MQ=255)
           aaCATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTtctc                                                                              >  2:4338607/1‑93 (MQ=255)
           aaCATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTtctc                                                                              >  2:2416332/1‑93 (MQ=255)
            aCATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCg                                                                             <  1:2696091/93‑1 (MQ=255)
            aCATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCg                                                                             >  1:5287653/1‑93 (MQ=255)
            aCATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCg                                                                             <  1:2887396/93‑1 (MQ=255)
             cATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCgg                                                                            >  1:810879/1‑93 (MQ=255)
               tCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGgag                                                                          >  2:6282552/1‑93 (MQ=255)
                 gACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGAc                                                                        >  2:2606148/1‑93 (MQ=255)
                 gACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGAc                                                                        >  2:1519229/1‑93 (MQ=255)
                   cacGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACaa                                                                      <  1:534798/93‑1 (MQ=255)
                   cacGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACaa                                                                      >  1:1218665/1‑93 (MQ=255)
                     cGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACaaaa                                                                    <  2:46051/93‑1 (MQ=255)
                      gATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACaaaaa                                                                   >  1:1599427/1‑93 (MQ=255)
                       aTCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAAc                                                                  >  2:1389171/1‑93 (MQ=255)
                       aTCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAAc                                                                  >  1:4166609/1‑93 (MQ=255)
                       aTCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAAc                                                                  <  2:1181790/93‑1 (MQ=255)
                         cGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAAcgc                                                                <  2:2625932/93‑1 (MQ=255)
                          gCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAAcgcc                                                               >  1:3079321/1‑93 (MQ=255)
                          gCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAAcgcc                                                               >  2:1303544/1‑93 (MQ=255)
                          gCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAAcgcc                                                               <  2:3698173/93‑1 (MQ=255)
                            cTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAAcgccgc                                                             >  1:4419960/1‑93 (MQ=255)
                             tGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACgccgcc                                                            >  1:4126583/1‑93 (MQ=255)
                             tGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACgccgcc                                                            >  2:620103/1‑93 (MQ=255)
                             tGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACgccgcc                                                            >  2:780613/1‑93 (MQ=255)
                               cgcgATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCtt                                                          <  1:4771581/93‑1 (MQ=255)
                                gcgATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTc                                                         <  2:5713213/93‑1 (MQ=255)
                                gcgATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTc                                                         <  2:4100/93‑1 (MQ=255)
                                gcgATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTc                                                         <  2:667200/93‑1 (MQ=255)
                                 cgATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCa                                                        <  1:4327154/93‑1 (MQ=255)
                                 cgATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCa                                                        <  1:5177906/93‑1 (MQ=255)
                                  gATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAg                                                       >  2:547674/1‑93 (MQ=255)
                                     ataGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCAt                                                    <  1:2188586/93‑1 (MQ=255)
                                      taGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATc                                                   >  2:5262935/1‑93 (MQ=255)
                                      taGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATc                                                   <  2:5286166/93‑1 (MQ=255)
                                       aGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATcc                                                  <  1:1097806/93‑1 (MQ=255)
                                         cGGGCGATCGGTAAAAATATACAAGCGGGGGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCtt                                                >  1:4364401/1‑93 (MQ=255)
                                          gggCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTa                                               <  1:6099293/93‑1 (MQ=255)
                                             cGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTAccc                                            >  1:1421057/1‑93 (MQ=255)
                                                tCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGa                                         <  1:3826981/93‑1 (MQ=255)
                                                gcGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGa                                         <  1:2551815/92‑1 (MQ=255)
                                                 cGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGaa                                        <  2:2109659/93‑1 (MQ=255)
                                                  ggTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAAt                                       >  2:2364836/1‑93 (MQ=255)
                                                  ggTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAAt                                       <  2:786494/93‑1 (MQ=255)
                                                   gTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATg                                      <  1:1530429/93‑1 (MQ=255)
                                                   gTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATg                                      <  1:3260294/93‑1 (MQ=255)
                                                    tAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGt                                     <  1:3660475/93‑1 (MQ=255)
                                                      aaaaTATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTAt                                   <  1:2914765/93‑1 (MQ=255)
                                                      aaaaTATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTAt                                   >  1:5729839/1‑93 (MQ=255)
                                                       aaaTATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATg                                  <  2:5781813/93‑1 (MQ=255)
                                                         atatACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGaa                                >  2:5629417/1‑93 (MQ=255)
                                                         atatACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGaa                                >  2:3106276/1‑93 (MQ=255)
                                                          tataCAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAAc                               <  1:4109760/93‑1 (MQ=255)
                                                           ataCAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACg                              >  1:320714/1‑93 (MQ=255)
                                                             aCAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGtt                            <  1:2375281/93‑1 (MQ=255)
                                                              cAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTc                           <  1:4676102/93‑1 (MQ=255)
                                                              cAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTc                           <  2:3599327/93‑1 (MQ=255)
                                                              cAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTc                           <  2:3585804/93‑1 (MQ=255)
                                                              cAAGCGGGTGTTGCAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTc                           <  1:3600320/93‑1 (MQ=255)
                                                               aaGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTGCCCAGAATGTATGAACGTTCt                          <  1:5804322/93‑1 (MQ=255)
                                                                aGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTg                         >  1:4983018/1‑93 (MQ=255)
                                                                aGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTg                         >  2:3905691/1‑93 (MQ=255)
                                                                 gCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTgg                        >  2:4808124/1‑93 (MQ=255)
                                                                 gCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTgg                        <  2:1666327/93‑1 (MQ=255)
                                                                 gCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTgg                        <  2:2864486/93‑1 (MQ=255)
                                                                 gCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTgg                        <  2:1875356/93‑1 (MQ=255)
                                                                 gCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACACAGAATGTATGAACGTTCTgg                        >  2:425428/1‑93 (MQ=255)
                                                                 gCGGGTGTTGTAGATTTCGCTTACGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTgg                        <  2:2945861/93‑1 (MQ=255)
                                                                   gggTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCg                      <  1:1042025/93‑1 (MQ=255)
                                                                   gggTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCg                      <  1:130835/93‑1 (MQ=255)
                                                                    ggTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGa                     >  1:5828228/1‑93 (MQ=255)
                                                                     gtgtTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGat                    >  2:1793657/1‑93 (MQ=255)
                                                                     gtgtTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGat                    >  2:1815018/1‑93 (MQ=255)
                                                                       gttgtAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGatat                  >  1:335386/1‑93 (MQ=255)
                                                                        ttgtAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGatata                 <  2:5583814/93‑1 (MQ=255)
                                                                          gtAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATAtg               >  1:1853763/1‑93 (MQ=255)
                                                                           tAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATAtgt              <  1:2530697/93‑1 (MQ=255)
                                                                            aGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCCGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATAtgtt             >  2:2998063/1‑93 (MQ=255)
                                                                            aGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAAAGTTCTGGCGCTATAtgtt             >  2:6248814/1‑93 (MQ=255)
                                                                              aTTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATAtgttgt           <  1:5686159/93‑1 (MQ=255)
                                                                               tttcgctttcgcAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTa          >  1:2823624/1‑93 (MQ=255)
                                                                                ttCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTaa         <  1:620103/93‑1 (MQ=255)
                                                                                ttCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTaa         <  2:5228770/93‑1 (MQ=255)
                                                                                   gCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTAACTg      >  1:2460575/1‑93 (MQ=255)
                                                                                    cTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTAACTGc     <  1:6282552/93‑1 (MQ=255)
                                                                                      ttCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTAACTGCaa   >  2:3369081/1‑93 (MQ=255)
                                                                                       tCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTAACTGCAAg  <  1:970030/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCGATCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGTAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTAACTGCAAG  >  NC_000913/2331326‑2331505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCCGATCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGTAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTAACTGCAAGGTA  >  NC_000913/2331323‑2331508
                                                                                             |                                                                                            
CGGCCGATCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGC                                                                                              >  SRR4302533.2196969/1‑94 (MQ=60)
  GCCGATCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAG                                                                                            <  SRR4302533.5926395/94‑1 (MQ=60)
   CCGATCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGA                                                                                           >  SRR4302533.2751132/1‑94 (MQ=60)
    CGATCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGGAGAA                                                                                          >  SRR4302533.5435633/1‑94 (MQ=60)
    CGATCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAA                                                                                          >  SRR4302533.2444410/1‑94 (MQ=60)
    CGATCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAA                                                                                          >  SRR4302533.1330229/1‑94 (MQ=60)
    CGATCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAA                                                                                          >  SRR4302533.3505775/1‑94 (MQ=60)
     GATCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAG                                                                                         <  SRR4302533.2763968/94‑1 (MQ=60)
       TCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAA                                                                                       <  SRR4302533.3009372/94‑1 (MQ=60)
       TCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAA                                                                                       >  SRR4302533.1905727/1‑94 (MQ=60)
        CACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAG                                                                                      <  SRR4302533.3919193/94‑1 (MQ=60)
         ACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGT                                                                                     <  SRR4302533.2907155/94‑1 (MQ=60)
            TTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCT                                                                                  >  SRR4302533.6129037/1‑94 (MQ=60)
              AACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCG                                                                                <  SRR4302533.427141/94‑1 (MQ=60)
              AACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCG                                                                                <  SRR4302533.4153017/94‑1 (MQ=60)
              AACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCG                                                                                >  SRR4302533.5319321/1‑94 (MQ=60)
               ACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGG                                                                               <  SRR4302533.2712613/94‑1 (MQ=60)
               ACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGG                                                                               <  SRR4302533.2905147/94‑1 (MQ=60)
               ACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGG                                                                               >  SRR4302533.815633/1‑94 (MQ=60)
                CATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGA                                                                              >  SRR4302533.700836/1‑94 (MQ=60)
                 ATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAG                                                                             <  SRR4302533.1839036/94‑1 (MQ=60)
                    GACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACA                                                                          <  SRR4302533.3002018/94‑1 (MQ=60)
                    GACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACA                                                                          >  SRR4302533.929231/1‑94 (MQ=60)
                     ACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAA                                                                         >  SRR4302533.1225982/1‑94 (MQ=60)
                      CACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAA                                                                        <  SRR4302533.5332520/94‑1 (MQ=60)
                      CACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAA                                                                        <  SRR4302533.537977/94‑1 (MQ=60)
                       ACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAA                                                                       >  SRR4302533.3609623/1‑94 (MQ=60)
                        CGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAA                                                                      <  SRR4302533.6305912/94‑1 (MQ=60)
                        CGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAA                                                                      <  SRR4302533.3299548/94‑1 (MQ=60)
                        CGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAA                                                                      >  SRR4302533.1609108/1‑94 (MQ=60)
                         GATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAAC                                                                     >  SRR4302533.4191858/1‑94 (MQ=60)
                            CGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCC                                                                  >  SRR4302533.3098218/1‑94 (MQ=60)
                              CCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGC                                                                >  SRR4302533.4446684/1‑94 (MQ=60)
                               CTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCC                                                               >  SRR4302533.5869579/1‑94 (MQ=60)
                               CTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCC                                                               >  SRR4302533.4151633/1‑94 (MQ=60)
                                TGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCT                                                              >  SRR4302533.1597981/1‑94 (MQ=60)
                                  CGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTC                                                            <  SRR4302533.2608906/94‑1 (MQ=60)
                                  CGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTC                                                            <  SRR4302533.4800500/94‑1 (MQ=60)
                                  CGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGGGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTC                                                            >  SRR4302533.120372/1‑94 (MQ=60)
                                    CGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAG                                                          <  SRR4302533.4353221/94‑1 (MQ=60)
                                    CGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAG                                                          <  SRR4302533.4137154/94‑1 (MQ=60)
                                    CGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAG                                                          <  SRR4302533.5209177/94‑1 (MQ=60)
                                    CGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAG                                                          <  SRR4302533.5749216/94‑1 (MQ=60)
                                    CGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAG                                                          <  SRR4302533.2835203/94‑1 (MQ=60)
                                        ATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATC                                                      <  SRR4302533.2202070/94‑1 (MQ=60)
                                         TAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCC                                                     <  SRR4302533.4046853/94‑1 (MQ=60)
                                          AGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCT                                                    <  SRR4302533.3424810/94‑1 (MQ=60)
                                          AGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCT                                                    <  SRR4302533.1104308/94‑1 (MQ=60)
                                           GCGGGCGATCGGTAAAAATATACAAGCGGGGGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTT                                                   >  SRR4302533.4390746/1‑94 (MQ=60)
                                            CGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTAGCAGAAGAAGTTCTCGGAGGCAAAAACGCCGCCTTCAGCATCCTTA                                                  >  SRR4302533.2339921/1‑94 (MQ=60)
                                             GGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTAC                                                 <  SRR4302533.6136127/94‑1 (MQ=60)
                                               GCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCC                                               <  SRR4302533.3110091/94‑1 (MQ=60)
                                               GCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCC                                               >  SRR4302533.1429653/1‑94 (MQ=60)
                                               GCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCC                                               >  SRR4302533.3627277/1‑94 (MQ=60)
                                                CGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCA                                              >  SRR4302533.5188133/1‑94 (MQ=60)
                                                CGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTCCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCA                                              <  SRR4302533.2480245/94‑1 (MQ=60)
                                                   TCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAA                                           <  SRR4302533.3850376/94‑1 (MQ=60)
                                                   GCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAA                                           <  SRR4302533.2567518/94‑1 (MQ=60)
                                                      GTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGT                                        <  SRR4302533.1539629/94‑1 (MQ=60)
                                                      GTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGT                                        <  SRR4302533.5853620/94‑1 (MQ=60)
                                                      GTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGT                                        <  SRR4302533.3280406/94‑1 (MQ=60)
                                                      GTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGT                                        >  SRR4302533.4330653/1‑94 (MQ=60)
                                                       TAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTA                                       <  SRR4302533.3682983/94‑1 (MQ=60)
                                                        AAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTAT                                      >  SRR4302533.5764439/1‑94 (MQ=60)
                                                        AAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTAT                                      >  SRR4302533.6218555/1‑94 (MQ=60)
                                                         AAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATG                                     <  SRR4302533.1365487/94‑1 (MQ=60)
                                                         AAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATG                                     <  SRR4302533.2932644/94‑1 (MQ=60)
                                                            ATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAAC                                  <  SRR4302533.286862/94‑1 (MQ=60)
                                                            ATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAAC                                  >  SRR4302533.3081330/1‑94 (MQ=60)
                                                            ATATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAAC                                  <  SRR4302533.4431426/94‑1 (MQ=60)
                                                             TATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACG                                 >  SRR4302533.322674/1‑94 (MQ=60)
                                                             TATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACG                                 <  SRR4302533.4134744/94‑1 (MQ=60)
                                                             TATACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACG                                 <  SRR4302533.1320306/94‑1 (MQ=60)
                                                                ACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTC                              <  SRR4302533.2561523/94‑1 (MQ=60)
                                                                ACAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTC                              <  SRR4302533.2389980/94‑1 (MQ=60)
                                                                 CAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCT                             <  SRR4302533.4704443/94‑1 (MQ=60)
                                                                 CAAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCT                             >  SRR4302533.3791201/1‑94 (MQ=60)
                                                                 CAAGCGGGTGTTGCAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCT                             <  SRR4302533.3622471/94‑1 (MQ=60)
                                                                  AAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTGCCCAGAATGTATGAACGTTCTG                            <  SRR4302533.5839376/94‑1 (MQ=60)
                                                                  AAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTG                            >  SRR4302533.5013232/1‑94 (MQ=60)
                                                                  AAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTG                            <  SRR4302533.540916/94‑1 (MQ=60)
                                                                  AAGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTG                            >  SRR4302533.6133759/1‑94 (MQ=60)
                                                                   AGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGG                           <  SRR4302533.6074102/94‑1 (MQ=60)
                                                                   AGCGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGG                           <  SRR4302533.1049966/94‑1 (MQ=60)
                                                                     GGGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCG                         <  SRR4302533.4861457/94‑1 (MQ=60)
                                                                      GGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGA                        <  SRR4302533.1048153/94‑1 (MQ=60)
                                                                      GGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGA                        <  SRR4302533.131655/94‑1 (MQ=60)
                                                                      GGGTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGA                        >  SRR4302533.5863445/1‑94 (MQ=60)
                                                                        GTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATA                      >  SRR4302533.4558271/1‑94 (MQ=60)
                                                                        GTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATA                      >  SRR4302533.2737821/1‑94 (MQ=60)
                                                                        GTGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATA                      >  SRR4302533.4165468/1‑94 (MQ=60)
                                                                         TGTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATAT                     >  SRR4302533.337436/1‑94 (MQ=60)
                                                                          GTTGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATA                    >  SRR4302533.3863513/1‑94 (MQ=60)
                                                                            TGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATG                  >  SRR4302533.1865064/1‑94 (MQ=60)
                                                                            TGTAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATG                  >  SRR4302533.4200555/1‑94 (MQ=60)
                                                                             GGAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGACCGTTCTGGCGATATATGT                 <  SRR4302533.3248194/94‑1 (MQ=60)
                                                                              TAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTT                <  SRR4302533.2546276/94‑1 (MQ=60)
                                                                              TAGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTT                <  SRR4302533.43898/94‑1 (MQ=60)
                                                                               AGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTG               <  SRR4302533.1265960/94‑1 (MQ=60)
                                                                               AGATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTG               <  SRR4302533.1700597/94‑1 (MQ=60)
                                                                                GATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGT              <  SRR4302533.5459239/94‑1 (MQ=60)
                                                                                 ATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTA             >  SRR4302533.2840999/1‑94 (MQ=60)
                                                                                 ATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTA             >  SRR4302533.5775258/1‑94 (MQ=60)
                                                                                 ATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTA             <  SRR4302533.5720503/94‑1 (MQ=60)
                                                                                 ATTTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTA             >  SRR4302533.1442750/1‑94 (MQ=60)
                                                                                   TTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTAAC           <  SRR4302533.5129596/94‑1 (MQ=60)
                                                                                   TTCGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTAAC           <  SRR4302533.623743/94‑1 (MQ=60)
                                                                                     CGCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTAACTG         >  SRR4302533.2475760/1‑94 (MQ=60)
                                                                                      GCTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCATCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTAACTGC        >  SRR4302533.3408055/1‑94 (MQ=60)
                                                                                       CTTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTAACTGCA       <  SRR4302533.6320046/94‑1 (MQ=60)
                                                                                        TTTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTAACTGCAA      >  SRR4302533.4711519/1‑94 (MQ=60)
                                                                                         TTCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTAACTGCAAG     <  SRR4302533.5306371/94‑1 (MQ=60)
                                                                                          TCGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTAACTGCAAGG    <  SRR4302533.975720/94‑1 (MQ=60)
                                                                                           CGCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTAACTGCAAGGT   >  SRR4302533.3547196/1‑94 (MQ=60)
                                                                                            GCAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTAACTGCAAGGTA  <  SRR4302533.5420165/94‑1 (MQ=60)
                                                                                             |                                                                                            
CGGCCGATCACTTTAACATCGACACGATCGCCTGCGCGATATAGCGGGCGATCGGTAAAAATATACAAGCGGGTGTTGTAGATTTCGCTTTCGTAGAAGAAGTTCTCGGAGACAAAAACGCCGCCTTCAGCATCCTTACCCAGAATGTATGAACGTTCTGGCGATATATGTTGTAACTGCAAGGTA  >  NC_000913/2331323‑2331508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑