Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,331,739 A→G intergenic (‑3922/+617) b2226 ← / ← b2229 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,331,7390AG99.6% 725.8 / ‑4.6 227intergenic (‑3922/+617)b2226/b2229hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (114/111);  total (114/113)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.31e-01

Only 100 of 234 total aligned reads displayed.

GTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTG  >  NC_000913/2331650‑2331828
                                                                                         |                                                                                         
gTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGata                                                                                        >  2:414316/1‑93 (MQ=255)
gTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGata                                                                                        <  1:1189280/93‑1 (MQ=255)
gTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGata                                                                                        >  1:789257/1‑93 (MQ=255)
gTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGata                                                                                        <  2:8845186/93‑1 (MQ=255)
    cGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATaaa                                                                                    <  1:678455/93‑1 (MQ=255)
    cGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATaaa                                                                                    >  1:4535405/1‑93 (MQ=255)
      gAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATg                                                                                  >  1:1378176/1‑93 (MQ=255)
       aaacaaacACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGt                                                                                 <  1:7337790/93‑1 (MQ=255)
       aaacaaacACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGt                                                                                 >  1:3902871/1‑93 (MQ=255)
        aacaaacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGtt                                                                                <  2:7449803/93‑1 (MQ=255)
         acagacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTa                                                                               >  2:1010051/1‑93 (MQ=255)
         acaaacaCCACCGTCGTCGCCCGATACCCACCAACTATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTa                                                                               <  2:8022336/93‑1 (MQ=255)
          caaacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTAc                                                                              <  1:7701286/93‑1 (MQ=255)
          caaacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTAc                                                                              >  2:7272771/1‑93 (MQ=255)
          caaacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTAc                                                                              >  2:1602289/1‑93 (MQ=255)
            aacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTAccc                                                                            <  2:5918948/93‑1 (MQ=255)
               cccaccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGc                                                                         <  1:6112815/92‑1 (MQ=255)
                ccaccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCt                                                                        >  1:1894887/1‑93 (MQ=255)
                  cccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTgc                                                                      <  1:263489/92‑1 (MQ=255)
                  cccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTgc                                                                      <  2:7338253/92‑1 (MQ=255)
                     gtcgtcGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTgcggc                                                                   >  2:5633055/1‑93 (MQ=255)
                      tcgtcgCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTgcggcg                                                                  <  1:1115991/93‑1 (MQ=255)
                        gtcgCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCgag                                                                >  2:8326614/1‑93 (MQ=255)
                         tcgCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCgaga                                                               <  2:3075156/93‑1 (MQ=255)
                         tcgCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCgaga                                                               <  2:2582649/93‑1 (MQ=255)
                            cccGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATGTAAATGTTACCCGGCTGCGGCGAGATGa                                                            >  1:1560529/1‑93 (MQ=255)
                              cGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGaaa                                                          <  2:4937599/93‑1 (MQ=255)
                              cGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGaaa                                                          <  1:5590686/93‑1 (MQ=255)
                               gATCCCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAAt                                                         <  1:913984/93‑1 (MQ=255)
                               gATACCCCCCAACCATCGCCTCGACGAGGAACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGCAGAGATGAAAt                                                         >  2:4209487/1‑93 (MQ=255)
                               gATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAAt                                                         >  2:8354476/1‑93 (MQ=255)
                                 tACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTg                                                       >  2:9739513/1‑93 (MQ=255)
                                 tACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTg                                                       >  2:5928033/1‑93 (MQ=255)
                                 tACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTg                                                       >  2:6110378/1‑93 (MQ=255)
                                 tACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAAATg                                                       >  2:295368/1‑93 (MQ=255)
                                  aCCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGc                                                      >  1:2022822/1‑93 (MQ=255)
                                   cccACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCt                                                     >  1:3917382/1‑93 (MQ=255)
                                    ccaccaACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTg                                                    >  1:4693176/1‑93 (MQ=255)
                                     caccaACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTgg                                                   >  2:3981089/1‑93 (MQ=255)
                                     caccaACCAGCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTgg                                                   <  2:5146082/93‑1 (MQ=255)
                                      accaaccaTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGa                                                  <  2:118839/93‑1 (MQ=255)
                                       ccaaccaTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATTTAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGAt                                                 >  2:4327551/1‑93 (MQ=255)
                                       ccaaccaTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGAt                                                 <  1:2761644/93‑1 (MQ=255)
                                       ccaaccaTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGAt                                                 <  1:4566868/93‑1 (MQ=255)
                                       ccaaccaTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGAt                                                 >  2:9691438/1‑93 (MQ=255)
                                       ccaaccaTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGAt                                                 >  1:602608/1‑93 (MQ=255)
                                       ccaaccaTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGAt                                                 <  2:6573861/93‑1 (MQ=255)
                                        caaccaTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACACGGCTGCGGCGAGATGAAATTGCTGGATg                                                >  1:7189583/1‑93 (MQ=255)
                                          accaTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATgcg                                              <  2:8760657/93‑1 (MQ=255)
                                            caTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGcc                                            <  1:6383940/93‑1 (MQ=255)
                                            caTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGcc                                            <  2:4795755/93‑1 (MQ=255)
                                             ctCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCt                                           <  1:2568892/92‑1 (MQ=255)
                                               cGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTc                                         <  2:2436295/93‑1 (MQ=255)
                                                gCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTcc                                        >  1:9828743/1‑93 (MQ=255)
                                                cccTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTcc                                        <  2:3259963/92‑1 (MQ=255)
                                                 ccTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCa                                       >  2:2480790/1‑93 (MQ=255)
                                                  cTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCAAg                                      >  1:3090102/1‑93 (MQ=255)
                                                   tCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGt                                     >  1:4285897/1‑93 (MQ=255)
                                                     gacgaGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGttt                                   <  1:3692311/93‑1 (MQ=255)
                                                      acgaGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGtttt                                  <  1:10040371/93‑1 (MQ=255)
                                                       cgaGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTa                                 >  2:4648980/1‑93 (MQ=255)
                                                        gaGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTACAGTTTTAc                                >  2:1484425/1‑93 (MQ=255)
                                                          ggTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTATCCGGCTGCGGCGTGACGAAATTGCTGGATGCGCCTGCCAGTTTTAcgc                              >  1:5857970/1‑91 (MQ=255)
                                                          ggTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTAccc                              >  2:3527890/1‑93 (MQ=255)
                                                           gTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTAcccc                             <  2:7493564/93‑1 (MQ=255)
                                                           gTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTAcccc                             <  1:4770349/93‑1 (MQ=255)
                                                            tACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGGGCCGTTCAGTTTTACCCCt                            >  2:4397955/1‑93 (MQ=255)
                                                            tACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCt                            >  2:6246611/1‑93 (MQ=255)
                                                            tACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCt                            <  2:7616860/93‑1 (MQ=255)
                                                              cAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTtg                          <  1:7619464/93‑1 (MQ=255)
                                                                gTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTtgct                        >  2:375005/1‑93 (MQ=255)
                                                                gTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTtgct                        >  1:3550153/1‑93 (MQ=255)
                                                                gTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTtgct                        >  1:8638395/1‑93 (MQ=255)
                                                                  cccGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTtgctgc                      <  1:6737705/93‑1 (MQ=255)
                                                                     ggCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGc                   >  1:7655339/1‑93 (MQ=255)
                                                                      gCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGGGGCt                  >  2:5236449/1‑93 (MQ=255)
                                                                       gtctTGTTGGCGGAGATGATTGTCAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTGCCCCTTGCTGCGGCTc                 <  2:5529596/92‑1 (MQ=255)
                                                                       ctctTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTc                 >  1:5297487/1‑93 (MQ=255)
                                                                        tctTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCg                >  1:6834868/1‑93 (MQ=255)
                                                                        tctTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCg                <  1:9310052/93‑1 (MQ=255)
                                                                        tctTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCg                >  2:6964434/1‑93 (MQ=255)
                                                                         ctTGTTGGCCGAGAGGGATATAAATGTTCCCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGa               <  1:6466174/93‑1 (MQ=255)
                                                                         ctTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGa               >  1:3594189/1‑93 (MQ=255)
                                                                         ctTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGa               >  1:3258121/1‑93 (MQ=255)
                                                                         ctTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGa               >  2:9079259/1‑93 (MQ=255)
                                                                         ctTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGa               >  1:1575325/1‑93 (MQ=255)
                                                                         ctTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGa               <  1:2456703/93‑1 (MQ=255)
                                                                          ttgttgGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGaa              <  1:6321086/93‑1 (MQ=255)
                                                                          ttgttgGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGaa              <  1:5415944/93‑1 (MQ=255)
                                                                             ttgGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACgg           <  2:3507411/93‑1 (MQ=255)
                                                                               ggCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGtt         <  2:2220907/93‑1 (MQ=255)
                                                                               ggCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGtt         <  2:8077778/93‑1 (MQ=255)
                                                                               ggCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGtt         <  1:3016470/93‑1 (MQ=255)
                                                                                gCCGAAAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGttt        <  1:853137/93‑1 (MQ=255)
                                                                                  cGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAg      >  1:4471412/1‑93 (MQ=255)
                                                                                   gagaGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGc     <  2:7854659/93‑1 (MQ=255)
                                                                                   gagaGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGGTTAGc     >  1:2238651/1‑93 (MQ=255)
                                                                                    agagGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTTGcc    >  1:8389607/1‑93 (MQ=255)
                                                                                    agagGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGcc    <  2:4179770/93‑1 (MQ=255)
                                                                                      agGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGCACGGCTTAACCTg  >  2:8559176/1‑93 (MQ=255)
                                                                                         |                                                                                         
GTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTG  >  NC_000913/2331650‑2331828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCAT  >  NC_000913/2331646‑2331832
                                                                                             |                                                                                             
CACGGTATCAGAAACAAACGCCACCGTCGTCGCCCGATCCCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGG                                                                                               <  SRR4302540.3790066/94‑1 (MQ=60)
   GGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATA                                                                                            >  SRR4302540.8636116/1‑94 (MQ=60)
   GGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATA                                                                                            >  SRR4302540.797643/1‑94 (MQ=60)
    GTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATAT                                                                                           <  SRR4302540.1201539/94‑1 (MQ=60)
    GTAGCGGACACAAACACCCCCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATAT                                                                                           <  SRR4302540.130906/94‑1 (MQ=60)
      ATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAA                                                                                         >  SRR4302540.3895120/1‑94 (MQ=60)
       TCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAA                                                                                        >  SRR4302540.4584498/1‑94 (MQ=60)
       TCGCAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAA                                                                                        <  SRR4302540.4599411/94‑1 (MQ=60)
        cgacaaaAAACACCACCGTCTTCCCCCGATACCCACCAACCATCGCCTCGATGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAAT                                                                                       <  SRR4302540.2339279/87‑1 (MQ=60)
        CGGAAACAAACCCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAAT                                                                                       <  SRR4302540.801926/94‑1 (MQ=60)
        CGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAAT                                                                                       <  SRR4302540.685718/94‑1 (MQ=60)
        CGGAAACAAACACCACCGTCGGCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAAT                                                                                       <  SRR4302540.2339280/94‑1 (MQ=60)
         GGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATG                                                                                      >  SRR4302540.7902341/1‑94 (MQ=60)
         GGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATG                                                                                      >  SRR4302540.1392850/1‑94 (MQ=60)
          GAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGT                                                                                     >  SRR4302540.3945204/1‑94 (MQ=60)
          GAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGT                                                                                     <  SRR4302540.4202519/94‑1 (MQ=60)
           AAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTT                                                                                    <  SRR4302540.3123088/94‑1 (MQ=60)
           AAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTT                                                                                    <  SRR4302540.7419677/94‑1 (MQ=60)
            AACAAACCCCACCGTCGTCGCCCGACACCCACCAACCATCGCCTCGACGATGTACAGTCCCGGCTTTTGTTGGCCGAGAGGGATATAAATGTTA                                                                                   <  SRR4302540.2603766/94‑1 (MQ=60)
              acaacacccaccgCGTCGCCCGATACCCCCCCACCATCGCCTCGACGAGGTACAGACCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACC                                                                                 <  SRR4302540.7789288/81‑1 (MQ=60)
              CAAACACCACCGTCGTCGCCCGATTCCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACC                                                                                 <  SRR4302540.3589210/94‑1 (MQ=60)
              CAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACC                                                                                 <  SRR4302540.7789287/94‑1 (MQ=60)
                  CACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGC                                                                             >  SRR4302540.3274466/1‑94 (MQ=60)
                   CCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCT                                                                            <  SRR4302540.6180887/94‑1 (MQ=60)
                   ACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCT                                                                            <  SRR4302540.4755155/94‑1 (MQ=60)
                   ACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCT                                                                            >  SRR4302540.1915625/1‑94 (MQ=60)
                     CACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGG                                                                          >  SRR4302540.2689645/1‑94 (MQ=60)
                     CACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGC                                                                          <  SRR4302540.1936969/94‑1 (MQ=60)
                      CCCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCG                                                                         <  SRR4302540.266059/94‑1 (MQ=60)
                      ACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCG                                                                         >  SRR4302540.7482971/1‑94 (MQ=60)
                      ACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCG                                                                         >  SRR4302540.6358388/1‑94 (MQ=60)
                          TCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGA                                                                     <  SRR4302540.1127557/94‑1 (MQ=60)
                           CGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAG                                                                    <  SRR4302540.9388169/94‑1 (MQ=60)
                              CGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATG                                                                 <  SRR4302540.3957848/94‑1 (MQ=60)
                               GCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATGTAAATGTTACCCGGCTGCGGCGAGATGA                                                                >  SRR4302540.1577665/1‑94 (MQ=60)
                                CCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGTCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAA                                                               >  SRR4302540.2412940/1‑94 (MQ=60)
                                  CGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAAT                                                             <  SRR4302540.5652205/94‑1 (MQ=60)
                                   GATCCCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATT                                                            <  SRR4302540.923652/94‑1 (MQ=60)
                                   GATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATT                                                            >  SRR4302540.7358350/1‑94 (MQ=60)
                                    ATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTG                                                           >  SRR4302540.6818933/1‑94 (MQ=60)
                                    ATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTG                                                           >  SRR4302540.2878708/1‑94 (MQ=60)
                                     TACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGC                                                          >  SRR4302540.2044683/1‑94 (MQ=60)
                                      ACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCT                                                         >  SRR4302540.3959795/1‑94 (MQ=60)
                                       CCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTG                                                        >  SRR4302540.4743351/1‑94 (MQ=60)
                                         CACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAGTGTTACCCGGCTGCGGCGAGATGAAATTGCTGGA                                                      >  SRR4302540.6285134/1‑94 (MQ=60)
                                         CACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGA                                                      <  SRR4302540.3329184/94‑1 (MQ=60)
                                          ACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGAT                                                     <  SRR4302540.5819993/94‑1 (MQ=60)
                                          ACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGAT                                                     >  SRR4302540.5803259/1‑94 (MQ=60)
                                          ACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGAT                                                     >  SRR4302540.609062/1‑94 (MQ=60)
                                           CCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATG                                                    >  SRR4302540.1693969/1‑94 (MQ=60)
                                           CCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATG                                                    <  SRR4302540.4616178/94‑1 (MQ=60)
                                           CCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATG                                                    <  SRR4302540.2791482/94‑1 (MQ=60)
                                           CCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATG                                                    <  SRR4302540.2791481/94‑1 (MQ=60)
                                           CCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACACGGCTGCGGCGCGTTGAAATTGCTGGATG                                                    >  SRR4302540.1693968/1‑94 (MQ=60)
                                           CCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACACGGCTGCGGCGAGATGAAATTGCTGGATG                                                    >  SRR4302540.7269719/1‑94 (MQ=60)
                                            CAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGC                                                   <  SRR4302540.1585005/94‑1 (MQ=60)
                                            CAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGC                                                   >  SRR4302540.3637615/1‑94 (MQ=60)
                                             AACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCG                                                  <  SRR4302540.5220330/94‑1 (MQ=60)
                                              ACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGC                                                 <  SRR4302540.9990693/94‑1 (MQ=60)
                                                CATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCT                                               <  SRR4302540.6455007/94‑1 (MQ=60)
                                                CATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCT                                               <  SRR4302540.6083668/94‑1 (MQ=60)
                                                 CTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTT                                              <  SRR4302540.2596590/94‑1 (MQ=60)
                                                 ATCTCCTCGGCGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTT                                              <  SRR4302540.2596589/94‑1 (MQ=60)
                                                   CGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCC                                            >  SRR4302540.9943711/1‑94 (MQ=60)
                                                    GCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCA                                           >  SRR4302540.4604131/1‑94 (MQ=60)
                                                     CCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAG                                          <  SRR4302540.4726511/94‑1 (MQ=60)
                                                     CCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAG                                          <  SRR4302540.4974336/94‑1 (MQ=60)
                                                     CCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCAAG                                          >  SRR4302540.3124002/1‑94 (MQ=60)
                                                      CTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGT                                         >  SRR4302540.4332519/1‑94 (MQ=60)
                                                        CGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTT                                       >  SRR4302540.8973952/1‑94 (MQ=60)
                                                         GACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTT                                      <  SRR4302540.3732637/94‑1 (MQ=60)
                                                         GACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGAGGCGCCTTCCAGTTTT                                      >  SRR4302540.7720972/1‑94 (MQ=60)
                                                         GACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCGTCGTGGAGATGAAATTGCTGGATGCGCCTTCCAGcttg                                      >  SRR4302540.7720971/1‑90 (MQ=60)
                                                          ACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTA                                     <  SRR4302540.10157116/94‑1 (MQ=60)
                                                            GAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACC                                   >  SRR4302540.3712320/1‑94 (MQ=60)
                                                            GAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACC                                   >  SRR4302540.656621/1‑94 (MQ=60)
                                                            GAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACC                                   <  SRR4302540.4473571/94‑1 (MQ=60)
                                                             AGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTATCCGGCTGCGGCGTGACGAAATTGCTGGATGCGCCTGCCAGTTTTACGC                                  >  SRR4302540.5922756/1‑94 (MQ=60)
                                                              GGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCC                                 >  SRR4302540.8919755/1‑94 (MQ=60)
                                                               GTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCT                                <  SRR4302540.4821306/94‑1 (MQ=60)
                                                                TACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTT                               <  SRR4302540.6502674/94‑1 (MQ=60)
                                                                TACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTT                               <  SRR4302540.6180339/94‑1 (MQ=60)
                                                                TACAGTCCCGGCCCTTGTTGGCCGAGAGGGATATAAATGGTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTT                               <  SRR4302540.7259792/94‑1 (MQ=60)
                                                                  CAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGC                             <  SRR4302540.7705141/94‑1 (MQ=60)
                                                                   AGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCT                            >  SRR4302540.8738740/1‑94 (MQ=60)
                                                                   AGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCT                            >  SRR4302540.3588940/1‑94 (MQ=60)
                                                                   AGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCT                            >  SRR4302540.5403496/1‑94 (MQ=60)
                                                                     TCCCGGCGCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGC                          <  SRR4302540.7989401/94‑1 (MQ=60)
                                                                      CCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCG                         >  SRR4302540.1188882/1‑94 (MQ=60)
                                                                      CCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCG                         <  SRR4302540.6812129/94‑1 (MQ=60)
                                                                       CCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGG                        >  SRR4302540.4049990/1‑94 (MQ=60)
                                                                        CGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGC                       >  SRR4302540.7741525/1‑94 (MQ=60)
                                                                          GCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTC                     >  SRR4302540.5355438/1‑94 (MQ=60)
                                                                           CTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCG                    >  SRR4302540.6910570/1‑94 (MQ=60)
                                                                            TCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGA                   >  SRR4302540.1592566/1‑94 (MQ=60)
                                                                            TCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGA                   <  SRR4302540.9421202/94‑1 (MQ=60)
                                                                            TCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGA                   >  SRR4302540.9471107/1‑94 (MQ=60)
                                                                            TCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGA                   >  SRR4302540.6877727/1‑94 (MQ=60)
                                                                            TCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGA                   >  SRR4302540.3633454/1‑94 (MQ=60)
                                                                            TCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGA                   >  SRR4302540.3293648/1‑94 (MQ=60)
                                                                            TCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCACTTGCTGCGGCTCGA                   >  SRR4302540.1026278/1‑94 (MQ=60)
                                                                             CTTGTTGGCCGAGAGGGATATAAATGTTCCCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAA                  <  SRR4302540.6538131/94‑1 (MQ=60)
                                                                             CTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAA                  >  SRR4302540.3246010/1‑94 (MQ=60)
                                                                             CTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAA                  <  SRR4302540.2483143/94‑1 (MQ=60)
                                                                              TTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAAC                 <  SRR4302540.5475497/94‑1 (MQ=60)
                                                                              TTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAAC                 <  SRR4302540.5589946/94‑1 (MQ=60)
                                                                              TTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAAC                 <  SRR4302540.6391474/94‑1 (MQ=60)
                                                                              TTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAAC                 <  SRR4302540.6391473/94‑1 (MQ=60)
                                                                               TGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACG                <  SRR4302540.9545680/94‑1 (MQ=60)
                                                                               TGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACG                <  SRR4302540.6711826/94‑1 (MQ=60)
                                                                                   GGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTT            <  SRR4302540.3049629/94‑1 (MQ=60)
                                                                                    GCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTA           <  SRR4302540.1560944/94‑1 (MQ=60)
                                                                                    GCCGAAAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTA           <  SRR4302540.862194/94‑1 (MQ=60)
                                                                                     CCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAG          >  SRR4302540.4519902/1‑94 (MQ=60)
                                                                                     CCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAG          <  SRR4302540.7103297/94‑1 (MQ=60)
                                                                                     CCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAG          <  SRR4302540.7764787/94‑1 (MQ=60)
                                                                                     CCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATACGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGGTTAG          >  SRR4302540.4519903/1‑94 (MQ=60)
                                                                                      CGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGCACGGTTTAGC         >  SRR4302540.2249677/1‑94 (MQ=60)
                                                                                      CGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGGTTAGC         >  SRR4302540.2262722/1‑94 (MQ=60)
                                                                                       GAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTTGCC        >  SRR4302540.8486953/1‑94 (MQ=60)
                                                                                        AGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCT       <  SRR4302540.8530330/94‑1 (MQ=60)
                                                                                        AGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCT       >  SRR4302540.1241143/1‑94 (MQ=60)
                                                                                           GGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTTGCCTGCC    >  SRR4302540.3044294/1‑94 (MQ=60)
                                                                                            GGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCA   >  SRR4302540.3208575/1‑94 (MQ=60)
                                                                                            GGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCA   >  SRR4302540.9367499/1‑94 (MQ=60)
                                                                                             GATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGACAGGTTTAGCCTGCCAA  >  SRR4302540.1476818/1‑94 (MQ=60)
                                                                                             GATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCCT  >  SRR4302540.8292622/1‑94 (MQ=60)
                                                                                             GATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCAT  <  SRR4302540.6117180/94‑1 (MQ=60)
                                                                                             GATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCAT  <  SRR4302540.2795514/94‑1 (MQ=60)
                                                                                             GATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCCAACGGTCTAGCCCGCCAA  >  SRR4302540.8292621/1‑94 (MQ=60)
                                                                                             |                                                                                             
CACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCAT  >  NC_000913/2331646‑2331832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑