Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,331,739 A→G intergenic (‑3922/+617) b2226 ← / ← b2229 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,331,7390AG100.0% 570.8 / NA 176intergenic (‑3922/+617)b2226/b2229hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (104/72);  total (104/72)

Only 100 of 182 total aligned reads displayed.

GGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCT  >  NC_000913/2331649‑2331827
                                                                                          |                                                                                        
ggTATCGGAAACAATCACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGat                                                                                        <  1:66653/93‑1 (MQ=255)
ggTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGat                                                                                        <  1:4575430/93‑1 (MQ=255)
ggTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGat                                                                                        <  1:2058133/93‑1 (MQ=255)
ggTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGat                                                                                        <  1:6137947/93‑1 (MQ=255)
 gTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGata                                                                                       <  2:4627464/93‑1 (MQ=255)
 gTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGata                                                                                       <  1:5452428/93‑1 (MQ=255)
 gTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGata                                                                                       >  2:4694880/1‑93 (MQ=255)
   aTCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGAtata                                                                                     >  1:3405588/1‑93 (MQ=255)
   aTCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGAtata                                                                                     <  2:5740617/93‑1 (MQ=255)
   aTCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGAACAGTCCCGGCCCTTGTTGGCCGAGAGGGAtata                                                                                     >  2:3860233/1‑93 (MQ=255)
    tCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCGTCGACGAGGCACAGTCCCCGGTCTTGTTGGCCGAGAGGGATATaa                                                                                    >  1:22019/1‑93 (MQ=255)
     cGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATaaa                                                                                   <  2:5604960/93‑1 (MQ=255)
     cGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATaaa                                                                                   >  1:3575857/1‑93 (MQ=255)
      ggAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAAt                                                                                  >  2:1830603/1‑93 (MQ=255)
       gAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATg                                                                                 >  2:3460187/1‑93 (MQ=255)
         aacaaacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGtt                                                                               >  2:3685352/1‑93 (MQ=255)
             aacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTAccc                                                                           <  1:501694/93‑1 (MQ=255)
                cccaccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGc                                                                        <  2:3377864/92‑1 (MQ=255)
                accaccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGc                                                                        <  1:5260510/93‑1 (MQ=255)
                  caccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTg                                                                      >  1:281635/1‑93 (MQ=255)
                  caccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTg                                                                      >  1:2473882/1‑93 (MQ=255)
                  caccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTg                                                                      <  2:4683146/93‑1 (MQ=255)
                   accGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTgc                                                                     >  1:4245740/1‑93 (MQ=255)
                    ccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTgcg                                                                    <  1:2099879/93‑1 (MQ=255)
                    ccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTgcg                                                                    >  1:4556781/1‑93 (MQ=255)
                      gtcgtcGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTTGCCGAGAGGGATATAAATGTTACCCGGCTgcggg                                                                  >  2:3259477/1‑92 (MQ=255)
                      gtcgtcGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCCGCTgcggc                                                                  >  1:3083833/1‑93 (MQ=255)
                       tcgtcgCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTgcggcg                                                                 >  1:4697910/1‑93 (MQ=255)
                         gtcgCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCgag                                                               >  1:634706/1‑93 (MQ=255)
                          tcgCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCgaga                                                              <  1:5717984/93‑1 (MQ=255)
                          tcgCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCgaga                                                              <  1:5173811/93‑1 (MQ=255)
                          tcgCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCgaga                                                              >  2:2877071/1‑93 (MQ=255)
                           cgCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGAt                                                             <  1:1631965/93‑1 (MQ=255)
                           cgCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGAt                                                             >  2:4949749/1‑93 (MQ=255)
                            gCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATg                                                            >  1:3801622/1‑93 (MQ=255)
                            gCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATg                                                            <  2:5520731/93‑1 (MQ=255)
                             cccGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGa                                                           >  2:1512902/1‑93 (MQ=255)
                             cccGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGa                                                           >  1:3381496/1‑93 (MQ=255)
                                gATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAAt                                                        >  2:1270110/1‑93 (MQ=255)
                                 aTACCCACCAACCATCGCCTCGACGATGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAAtt                                                       <  2:3156450/93‑1 (MQ=255)
                                  tACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTg                                                      >  1:1423539/1‑93 (MQ=255)
                                  tACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTg                                                      >  1:1440183/1‑93 (MQ=255)
                                    cccACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCt                                                    <  2:4171169/93‑1 (MQ=255)
                                    cccACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCt                                                    >  1:5499952/1‑93 (MQ=255)
                                      caccaACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTgg                                                  >  1:116803/1‑93 (MQ=255)
                                      caccaACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTgg                                                  <  1:4256230/93‑1 (MQ=255)
                                      caccaACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTgg                                                  >  1:3640743/1‑93 (MQ=255)
                                       accaaccaTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGa                                                 <  1:3034471/93‑1 (MQ=255)
                                       accaaccaTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGa                                                 <  1:5412425/93‑1 (MQ=255)
                                       accaaccaTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGa                                                 <  2:1041997/93‑1 (MQ=255)
                                          aaccaTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATgc                                              <  2:4053011/93‑1 (MQ=255)
                                             caTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGcc                                           <  1:5213910/93‑1 (MQ=255)
                                                cGCCTCGACGAGGAACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTc                                        >  1:3903588/1‑93 (MQ=255)
                                                 gCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTcc                                       >  1:5398841/1‑93 (MQ=255)
                                                  ccTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCa                                      <  1:1035405/93‑1 (MQ=255)
                                                   cTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAg                                     <  2:5062543/93‑1 (MQ=255)
                                                    tCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGt                                    >  2:5873730/1‑93 (MQ=255)
                                                    tCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGt                                    >  2:5673549/1‑93 (MQ=255)
                                                    tCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGt                                    >  1:473670/1‑93 (MQ=255)
                                                      gacgaGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGttt                                  >  1:2235656/1‑93 (MQ=255)
                                                      gacgaGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGttt                                  <  2:5891746/93‑1 (MQ=255)
                                                       acgaGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGtttt                                 <  1:3151661/93‑1 (MQ=255)
                                                       acgaGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGtttt                                 >  1:120974/1‑93 (MQ=255)
                                                        cgaGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTa                                >  2:1208881/1‑93 (MQ=255)
                                                         gaGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTAc                               >  1:4992201/1‑93 (MQ=255)
                                                           ggTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTAccc                             >  2:764416/1‑93 (MQ=255)
                                                             tACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTTCCCCt                           <  2:173930/93‑1 (MQ=255)
                                                              aCAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCtt                          >  1:5109269/1‑93 (MQ=255)
                                                              aCAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCtt                          <  2:2721119/93‑1 (MQ=255)
                                                               cAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTtg                         <  1:4407041/93‑1 (MQ=255)
                                                               cAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTtg                         >  1:2885055/1‑93 (MQ=255)
                                                                aGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTtgc                        <  1:1109090/93‑1 (MQ=255)
                                                                aGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTtgc                        <  2:3952702/93‑1 (MQ=255)
                                                                  tCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTtgctg                      >  2:5253415/1‑93 (MQ=255)
                                                                   cccGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGAGGCGAGATGAAATAGCTGGATGCGCCTTCCAGTTTTACCCCTtgctgc                     >  1:1509005/1‑93 (MQ=255)
                                                                   cccGGCTCTTGTTGGCCGAGAGGGATATAAATGGTACTCGGCCGCGGCGAGAAGATTATGTAGGAGGCGCCTTCCTGGTATACCCCTtactgc                     >  2:6301253/1‑93 (MQ=255)
                                                                    ccGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCg                    >  1:3243593/1‑93 (MQ=255)
                                                                    ccGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCg                    <  2:5476556/93‑1 (MQ=255)
                                                                    ccGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCg                    >  1:5956471/1‑93 (MQ=255)
                                                                     cGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCgg                   >  2:953439/1‑93 (MQ=255)
                                                                     cGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCgg                   <  2:1364815/93‑1 (MQ=255)
                                                                      ggCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGc                  <  1:2354513/93‑1 (MQ=255)
                                                                      ggCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGc                  >  2:3406229/1‑93 (MQ=255)
                                                                       gCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCt                 >  1:4678170/1‑93 (MQ=255)
                                                                       gCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCt                 >  1:3834466/1‑93 (MQ=255)
                                                                       gCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCt                 <  2:2061755/93‑1 (MQ=255)
                                                                        ctctTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTc                >  2:5361272/1‑93 (MQ=255)
                                                                         tctTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCg               >  1:3173932/1‑93 (MQ=255)
                                                                          ctTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGa              >  1:980067/1‑93 (MQ=255)
                                                                          ctTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGa              <  1:4422545/93‑1 (MQ=255)
                                                                           ttgttgGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGaa             <  1:1285555/93‑1 (MQ=255)
                                                                            tgttgGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAAc            <  2:6182866/93‑1 (MQ=255)
                                                                               tgGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGt         >  2:2862644/1‑93 (MQ=255)
                                                                                ggCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGtt        <  2:4020630/93‑1 (MQ=255)
                                                                                ggCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCCAACGGtt        >  1:933629/1‑93 (MQ=255)
                                                                                 gCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGttt       <  2:2556777/93‑1 (MQ=255)
                                                                                  ccGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTa      <  2:4129803/93‑1 (MQ=255)
                                                                                  ccGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTa      >  2:3008031/1‑93 (MQ=255)
                                                                                   cGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAg     >  1:6180910/1‑93 (MQ=255)
                                                                                    gagaGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGc    >  2:4337469/1‑93 (MQ=255)
                                                                                          |                                                                                        
GGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCT  >  NC_000913/2331649‑2331827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCA  >  NC_000913/2331646‑2331831
                                                                                             |                                                                                            
CACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGG                                                                                              <  SRR4302533.1037836/94‑1 (MQ=60)
CACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGG                                                                                              <  SRR4302533.979637/94‑1 (MQ=60)
CACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGG                                                                                              <  SRR4302533.1839613/94‑1 (MQ=60)
CACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGG                                                                                              >  SRR4302533.4391945/1‑94 (MQ=60)
  CGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGAT                                                                                            >  SRR4302533.758019/1‑94 (MQ=60)
  CGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGAT                                                                                            >  SRR4302533.5428690/1‑94 (MQ=60)
   GGTATCGGAAACAATCACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATA                                                                                           <  SRR4302533.67087/94‑1 (MQ=60)
   GGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATA                                                                                           <  SRR4302533.4603157/94‑1 (MQ=60)
   GGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATA                                                                                           <  SRR4302533.2070823/94‑1 (MQ=60)
   GGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATA                                                                                           <  SRR4302533.6174921/94‑1 (MQ=60)
   GGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATA                                                                                           <  SRR4302533.939582/94‑1 (MQ=60)
    GTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATAT                                                                                          <  SRR4302533.5485271/94‑1 (MQ=60)
    GTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATAT                                                                                          >  SRR4302533.2035463/1‑94 (MQ=60)
     TATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATA                                                                                         >  SRR4302533.3426621/1‑94 (MQ=60)
     TATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATA                                                                                         <  SRR4302533.3581873/94‑1 (MQ=60)
      ATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCGTCGACGAGGCACAGTCCCCGGTCTTGTTGGCCGAGAGGGATATAA                                                                                        >  SRR4302533.22166/1‑94 (MQ=60)
       TCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAA                                                                                       >  SRR4302533.3597897/1‑94 (MQ=60)
        CGAAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAAT                                                                                      <  SRR4302533.5514396/94‑1 (MQ=60)
         GGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATG                                                                                     >  SRR4302533.443414/1‑94 (MQ=60)
         GGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGAAGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATG                                                                                     <  SRR4302533.3191210/94‑1 (MQ=60)
          GAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGT                                                                                    >  SRR4302533.2881962/1‑94 (MQ=60)
           AAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTT                                                                                   >  SRR4302533.5632633/1‑94 (MQ=60)
                AACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCG                                                                              >  SRR4302533.3330709/1‑94 (MQ=60)
                AACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCG                                                                              <  SRR4302533.3125367/94‑1 (MQ=60)
                AACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCG                                                                              >  SRR4302533.4849795/1‑94 (MQ=60)
                AACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCG                                                                              <  SRR4302533.504676/94‑1 (MQ=60)
                  CACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGC                                                                            >  SRR4302533.2208582/1‑94 (MQ=60)
                   ACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCT                                                                           <  SRR4302533.5292091/94‑1 (MQ=60)
                    CCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTG                                                                          >  SRR4302533.2489137/1‑94 (MQ=60)
                    CCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTG                                                                          >  SRR4302533.283357/1‑94 (MQ=60)
                     CACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGC                                                                         >  SRR4302533.4271371/1‑94 (MQ=60)
                      CCCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCG                                                                        <  SRR4302533.1236765/94‑1 (MQ=60)
                      ACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCG                                                                        >  SRR4302533.4584415/1‑94 (MQ=60)
                       CCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGG                                                                       <  SRR4302533.2112831/94‑1 (MQ=60)
                        CGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCCGCTGCGGC                                                                      >  SRR4302533.3102791/1‑94 (MQ=60)
                         GTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCG                                                                     >  SRR4302533.1491262/1‑94 (MQ=60)
                         GTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCG                                                                     >  SRR4302533.4726387/1‑94 (MQ=60)
                           CGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAG                                                                   >  SRR4302533.6281373/1‑94 (MQ=60)
                           CGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAG                                                                   >  SRR4302533.638438/1‑94 (MQ=60)
                             TCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGAT                                                                 <  SRR4302533.5205066/94‑1 (MQ=60)
                             TCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGAT                                                                 <  SRR4302533.5752515/94‑1 (MQ=60)
                              CGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATG                                                                >  SRR4302533.603147/1‑94 (MQ=60)
                              CGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATG                                                                <  SRR4302533.1641850/94‑1 (MQ=60)
                              CGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATG                                                                >  SRR4302533.3824887/1‑94 (MQ=60)
                               GCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGA                                                               >  SRR4302533.3402370/1‑94 (MQ=60)
                                 CCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAA                                                             >  SRR4302533.3608113/1‑94 (MQ=60)
                                   GATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTTGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATT                                                           >  SRR4302533.2688652/1‑94 (MQ=60)
                                   GATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATT                                                           <  SRR4302533.4464962/94‑1 (MQ=60)
                                    ATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTG                                                          >  SRR4302533.1448872/1‑94 (MQ=60)
                                    ATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTG                                                          >  SRR4302533.1432150/1‑94 (MQ=60)
                                      ACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCT                                                        >  SRR4302533.5533130/1‑94 (MQ=60)
                                        CCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGG                                                      >  SRR4302533.3663132/1‑94 (MQ=60)
                                        CCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGG                                                      <  SRR4302533.527536/94‑1 (MQ=60)
                                        CCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGG                                                      >  SRR4302533.117548/1‑94 (MQ=60)
                                         CACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGA                                                     <  SRR4302533.4281891/94‑1 (MQ=60)
                                          ACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGAT                                                    <  SRR4302533.5444971/94‑1 (MQ=60)
                                          ACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGAT                                                    <  SRR4302533.4049879/94‑1 (MQ=60)
                                          ACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGAT                                                    <  SRR4302533.3053039/94‑1 (MQ=60)
                                          ACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGAT                                                    <  SRR4302533.5463193/94‑1 (MQ=60)
                                                CATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCT                                              <  SRR4302533.5245307/94‑1 (MQ=60)
                                                  TCGCCTCGACGAGGAACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTC                                            >  SRR4302533.3927429/1‑94 (MQ=60)
                                                   CGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCC                                           >  SRR4302533.5431284/1‑94 (MQ=60)
                                                     CCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAG                                         <  SRR4302533.661272/94‑1 (MQ=60)
                                                     CCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAG                                         <  SRR4302533.1041493/94‑1 (MQ=60)
                                                      CTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGT                                        >  SRR4302533.476500/1‑94 (MQ=60)
                                                      CTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGT                                        >  SRR4302533.3815412/1‑94 (MQ=60)
                                                       TCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTT                                       >  SRR4302533.4271941/1‑94 (MQ=60)
                                                       TCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTT                                       <  SRR4302533.3841441/94‑1 (MQ=60)
                                                        CGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTT                                      >  SRR4302533.2249478/1‑94 (MQ=60)
                                                         GACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTT                                     >  SRR4302533.121738/1‑94 (MQ=60)
                                                          ACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTA                                    >  SRR4302533.2100504/1‑94 (MQ=60)
                                                          ACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTA                                    <  SRR4302533.5063869/94‑1 (MQ=60)
                                                          ACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTA                                    <  SRR4302533.3171036/94‑1 (MQ=60)
                                                           CGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTAC                                   >  SRR4302533.1342555/1‑94 (MQ=60)
                                                           CGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTAC                                   >  SRR4302533.5022466/1‑94 (MQ=60)
                                                           AGAGGTACAGTCCCGGCTCTTGTTGGTCGAGAGGGATATAAATGTTACCCGGGTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTAC                                   <  SRR4302533.4748741/94‑1 (MQ=60)
                                                            GAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACC                                  >  SRR4302533.3226488/1‑94 (MQ=60)
                                                            GAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACC                                  >  SRR4302533.3078370/1‑94 (MQ=60)
                                                               GTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCT                               >  SRR4302533.113962/1‑94 (MQ=60)
                                                               GTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCT                               <  SRR4302533.5192736/94‑1 (MQ=60)
                                                                TACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTT                              >  SRR4302533.5140143/1‑94 (MQ=60)
                                                                 ACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTG                             >  SRR4302533.2902793/1‑94 (MQ=60)
                                                                  CAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGC                            <  SRR4302533.4433652/94‑1 (MQ=60)
                                                                   AGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCT                           <  SRR4302533.1115673/94‑1 (MQ=60)
                                                                    GTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTG                          <  SRR4302533.4709781/94‑1 (MQ=60)
                                                                     TCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGAGGCGAGATGAAATAGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGC                         >  SRR4302533.1518103/1‑94 (MQ=60)
                                                                      CCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCG                        >  SRR4302533.3263583/1‑94 (MQ=60)
                                                                      CCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCG                        >  SRR4302533.1530759/1‑94 (MQ=60)
                                                                      CCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCG                        >  SRR4302533.5992503/1‑94 (MQ=60)
                                                                        CGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGC                      >  SRR4302533.4356195/1‑94 (MQ=60)
                                                                         GGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCT                     >  SRR4302533.3857898/1‑94 (MQ=60)
                                                                         GGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCT                     <  SRR4302533.2369074/94‑1 (MQ=60)
                                                                         GGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCT                     >  SRR4302533.4706521/1‑94 (MQ=60)
                                                                         GGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCT                     >  SRR4302533.577194/1‑94 (MQ=60)
                                                                           CTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCG                   >  SRR4302533.3193466/1‑94 (MQ=60)
                                                                           CTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCG                   >  SRR4302533.1199040/1‑94 (MQ=60)
                                                                           CTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCG                   >  SRR4302533.5231259/1‑94 (MQ=60)
                                                                            TCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGA                  >  SRR4302533.985815/1‑94 (MQ=60)
                                                                             CTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAA                 <  SRR4302533.4449284/94‑1 (MQ=60)
                                                                             CTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCCAA                 >  SRR4302533.2475358/1‑94 (MQ=60)
                                                                              TTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAAC                <  SRR4302533.1293287/94‑1 (MQ=60)
                                                                                  TGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCCAACGGTT            >  SRR4302533.939107/1‑94 (MQ=60)
                                                                                    GCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTA          <  SRR4302533.2354224/94‑1 (MQ=60)
                                                                                     CCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAG         >  SRR4302533.6218030/1‑94 (MQ=60)
                                                                                         GAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTG     <  SRR4302533.4607557/94‑1 (MQ=60)
                                                                                          AGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGC    >  SRR4302533.6336992/1‑94 (MQ=60)
                                                                                           GGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCC   <  SRR4302533.5987474/94‑1 (MQ=60)
                                                                                           GGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCC   <  SRR4302533.1530172/94‑1 (MQ=60)
                                                                                            GGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCA  <  SRR4302533.4074785/94‑1 (MQ=60)
                                                                                             |                                                                                            
CACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCA  >  NC_000913/2331646‑2331831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑