Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,356,631 T→C S79G (AGT→GGT)  b2245 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,356,6310TC100.0% 739.1 / NA 226S79G (AGT→GGT) b2245hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (117/109);  total (117/109)

Only 100 of 233 total aligned reads displayed.

CCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGT  >  NC_000913/2356541‑2356720
                                                                                          |                                                                                         
ccaccTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCTCGCCAATATCCAGGACTTGTTTAATCAGCGGATTACCGc                                                                                         >  2:1045172/1‑93 (MQ=255)
 caccTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGcc                                                                                        <  2:5691632/93‑1 (MQ=255)
 caccTGACGTGCCTGTTCGGCAGTATCAACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGcc                                                                                        >  2:514210/1‑93 (MQ=255)
  accTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCt                                                                                       >  2:6030057/1‑93 (MQ=255)
    cTNACGNGCCTGTTCGGCAGTNNCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTc                                                                                     <  1:5638479/93‑1 (MQ=255)
    cTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTc                                                                                     <  2:6225641/93‑1 (MQ=255)
    cTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTc                                                                                     >  1:5455759/1‑93 (MQ=255)
      gACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTATCGCCTTCCa                                                                                   >  2:2213159/1‑93 (MQ=255)
      gACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCa                                                                                   <  1:4836510/93‑1 (MQ=255)
      gACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCa                                                                                   <  2:1230572/93‑1 (MQ=255)
       gcgtgcgtgTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCAc                                                                                  <  2:5477972/92‑1 (MQ=255)
          tGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACcgg                                                                               <  1:1724841/93‑1 (MQ=255)
            ccTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACcggac                                                                             >  1:3630479/1‑93 (MQ=255)
            ccTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACcggac                                                                             <  1:3829707/93‑1 (MQ=255)
            ccTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACcggac                                                                             <  2:4868473/93‑1 (MQ=255)
              tGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACcggacgg                                                                           <  2:1629656/93‑1 (MQ=255)
               gTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACcggacgga                                                                          <  1:3085/93‑1 (MQ=255)
                ttCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGAt                                                                         >  2:1486121/1‑93 (MQ=255)
                 tCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATc                                                                        >  2:3377644/1‑93 (MQ=255)
                  cGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCa                                                                       <  1:5731647/93‑1 (MQ=255)
                   ggCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCAc                                                                      >  1:5020827/1‑93 (MQ=255)
                      aGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTAGCGCCTTCCACCGGACGGATCACggg                                                                   >  2:933905/1‑93 (MQ=255)
                      aGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACggg                                                                   <  2:1990992/93‑1 (MQ=255)
                       gTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGt                                                                  <  1:3735253/93‑1 (MQ=255)
                       gTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGt                                                                  <  2:3100279/93‑1 (MQ=255)
                       gTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGt                                                                  >  1:1517773/1‑93 (MQ=255)
                          tCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTtgg                                                               >  2:526945/1‑93 (MQ=255)
                          tCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTtgg                                                               >  1:5381558/1‑93 (MQ=255)
                           cGACCATCGGGATCAGTAGAGTTCGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTtggc                                                              <  1:1303699/93‑1 (MQ=255)
                           cGACCATCGGGATCAGTAAAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTtggc                                                              <  2:3459595/93‑1 (MQ=255)
                              ccATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTtggctgg                                                           >  1:4446965/1‑93 (MQ=255)
                               cATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTtggctggc                                                          >  1:5093041/1‑93 (MQ=255)
                                aTCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCa                                                         <  1:1148116/93‑1 (MQ=255)
                                  cGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATa                                                       <  2:1011852/93‑1 (MQ=255)
                                   gggATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAg                                                      <  1:1468239/93‑1 (MQ=255)
                                    ggATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAgg                                                     <  2:3872191/93‑1 (MQ=255)
                                    ggATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAgg                                                     >  1:4096572/1‑93 (MQ=255)
                                     gATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAggg                                                    <  2:2448848/93‑1 (MQ=255)
                                     gATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAggg                                                    <  2:4426356/93‑1 (MQ=255)
                                     gATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAggg                                                    <  1:5948768/93‑1 (MQ=255)
                                      aTCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGgc                                                   >  2:796640/1‑93 (MQ=255)
                                       tCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGgcg                                                  >  2:4077583/1‑93 (MQ=255)
                                       tCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGGTGGCTGGCATAGGgcg                                                  >  1:3324508/1‑93 (MQ=255)
                                        cAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGgcgc                                                 <  2:3993328/93‑1 (MQ=255)
                                         aGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCt                                                <  1:2196172/93‑1 (MQ=255)
                                         aGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCt                                                <  1:2787241/93‑1 (MQ=255)
                                         aGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCt                                                >  2:4154767/1‑93 (MQ=255)
                                          gTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTa                                               >  2:5077732/1‑93 (MQ=255)
                                           tAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTAc                                              <  1:3126031/93‑1 (MQ=255)
                                           tAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTAc                                              >  2:3319207/1‑93 (MQ=255)
                                           tAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTAc                                              <  2:3206500/93‑1 (MQ=255)
                                             gagTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCg                                            >  2:4768722/1‑93 (MQ=255)
                                               gTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGcc                                          >  1:3011762/1‑93 (MQ=255)
                                               gTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGcc                                          <  2:5555633/93‑1 (MQ=255)
                                                tttGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCt                                         <  2:663884/93‑1 (MQ=255)
                                                  tGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGt                                       <  2:5647918/93‑1 (MQ=255)
                                                  tGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGt                                       <  2:2536631/93‑1 (MQ=255)
                                                   gcgcgcCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTa                                      <  1:4798437/93‑1 (MQ=255)
                                                     gcgcCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGc                                    <  2:2927750/93‑1 (MQ=255)
                                                     gcgcCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGc                                    <  1:3905181/93‑1 (MQ=255)
                                                        ccAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCtga                                 >  2:1278081/1‑93 (MQ=255)
                                                         cAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCtgat                                <  1:2727456/93‑1 (MQ=255)
                                                          aaTATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCtgatg                               >  1:3410238/1‑93 (MQ=255)
                                                            tatCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTgatgat                             <  2:4661913/93‑1 (MQ=255)
                                                             atCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATa                            >  1:6055896/1‑93 (MQ=255)
                                                             atCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATa                            <  2:3023434/93‑1 (MQ=255)
                                                             atCCAGGACTTGTTTAAACAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATa                            >  1:875104/1‑93 (MQ=255)
                                                               ccAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATaaa                          >  1:405475/1‑93 (MQ=255)
                                                               ccAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATaaa                          >  1:581898/1‑93 (MQ=255)
                                                                cAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAg                         >  2:1859025/1‑93 (MQ=255)
                                                                cAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAg                         >  1:2323707/1‑93 (MQ=255)
                                                                 aGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATATGGCGCTACCGCCTGTAGCTGATGATAAAGa                        >  2:609685/1‑93 (MQ=255)
                                                                 aGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGa                        <  1:1790020/93‑1 (MQ=255)
                                                                 aGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGa                        >  2:639787/1‑93 (MQ=255)
                                                                 aGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGa                        >  2:429567/1‑93 (MQ=255)
                                                                 aGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGa                        <  1:100161/93‑1 (MQ=255)
                                                                   gACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATc                      >  1:5846283/1‑93 (MQ=255)
                                                                   gACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATc                      >  2:86883/1‑93 (MQ=255)
                                                                     cTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCt                    >  1:1632658/1‑93 (MQ=255)
                                                                     cTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCt                    >  1:1913436/1‑93 (MQ=255)
                                                                     cTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCt                    >  1:689031/1‑93 (MQ=255)
                                                                     cTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCt                    <  1:1679962/93‑1 (MQ=255)
                                                                      ttGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTg                   >  1:3685793/1‑93 (MQ=255)
                                                                      ttGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTg                   >  2:4525623/1‑93 (MQ=255)
                                                                       tGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGa                  >  2:117871/1‑93 (MQ=255)
                                                                        gTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGaa                 >  1:3864013/1‑93 (MQ=255)
                                                                         tttAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAAt                >  1:4726424/1‑93 (MQ=255)
                                                                            aaTCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGgt             >  1:6152046/1‑93 (MQ=255)
                                                                               cAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGtt          <  1:2520221/93‑1 (MQ=255)
                                                                                aGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATGCTGAATGGTGttt         >  1:3210618/1‑93 (MQ=255)
                                                                                aGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGttt         <  2:1603208/93‑1 (MQ=255)
                                                                                aGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGttt         <  1:2261342/93‑1 (MQ=255)
                                                                                aGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGttt         <  2:5719609/93‑1 (MQ=255)
                                                                                 gCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTg        >  2:3500967/1‑93 (MQ=255)
                                                                                 gCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAAGGGTGTTTg        <  2:6134922/93‑1 (MQ=255)
                                                                                  ctgTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTgg       <  2:2032371/91‑1 (MQ=255)
                                                                                  cGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTgg       <  2:2120136/93‑1 (MQ=255)
                                                                                    gTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGgcg     >  2:2144985/1‑93 (MQ=255)
                                                                                     tttACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGgcgc    >  2:2314206/1‑93 (MQ=255)
                                                                                       tACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGt  <  2:282146/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGT  >  NC_000913/2356541‑2356720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCT  >  NC_000913/2356538‑2356723
                                                                                             |                                                                                            
ACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACC                                                                                              <  SRR4302533.5175353/94‑1 (MQ=60)
ACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACC                                                                                              <  SRR4302533.2454684/94‑1 (MQ=60)
ACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACC                                                                                              <  SRR4302533.2261150/94‑1 (MQ=60)
ACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACC                                                                                              <  SRR4302533.4839728/94‑1 (MQ=60)
  ACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGC                                                                                            >  SRR4302533.3039291/1‑94 (MQ=60)
    CACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCT                                                                                          <  SRR4302533.3689868/94‑1 (MQ=60)
     ACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTT                                                                                         >  SRR4302533.3722339/1‑94 (MQ=60)
      CCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTC                                                                                        >  SRR4302533.4939803/1‑94 (MQ=60)
      CCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTC                                                                                        >  SRR4302533.5488627/1‑94 (MQ=60)
       CTNACGNGCCTGTTCGGCAGTNNCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCC                                                                                       <  SRR4302533.5672569/94‑1 (MQ=60)
        TGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCA                                                                                      <  SRR4302533.543463/94‑1 (MQ=60)
         GACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCAC                                                                                     <  SRR4302533.4865826/94‑1 (MQ=60)
            GTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGG                                                                                  >  SRR4302533.765265/1‑94 (MQ=60)
             TGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGA                                                                                 <  SRR4302533.1735307/94‑1 (MQ=60)
             TGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGA                                                                                 >  SRR4302533.834861/1‑94 (MQ=60)
              GCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGAC                                                                                >  SRR4302533.3652816/1‑94 (MQ=60)
               CCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACG                                                                               <  SRR4302533.2952592/94‑1 (MQ=60)
               CCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACG                                                                               <  SRR4302533.3853120/94‑1 (MQ=60)
                CTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGG                                                                              >  SRR4302533.1579720/1‑94 (MQ=60)
                CTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGG                                                                              >  SRR4302533.5798621/1‑94 (MQ=60)
                  GTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGAT                                                                            <  SRR4302533.3110/94‑1 (MQ=60)
                     CGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCAC                                                                         >  SRR4302533.5051235/1‑94 (MQ=60)
                     CGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCAC                                                                         <  SRR4302533.5766251/94‑1 (MQ=60)
                         AGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGT                                                                     >  SRR4302533.6118108/1‑94 (MQ=60)
                         AGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGT                                                                     >  SRR4302533.1526908/1‑94 (MQ=60)
                          GTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTT                                                                    >  SRR4302533.3525252/1‑94 (MQ=60)
                          GTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTT                                                                    <  SRR4302533.3758168/94‑1 (MQ=60)
                          GTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTT                                                                    <  SRR4302533.3857190/94‑1 (MQ=60)
                           TATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTG                                                                   <  SRR4302533.3545999/94‑1 (MQ=60)
                            ATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGG                                                                  >  SRR4302533.5413890/1‑94 (MQ=60)
                            ATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGG                                                                  <  SRR4302533.585844/94‑1 (MQ=60)
                            ATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGG                                                                  >  SRR4302533.4508325/1‑94 (MQ=60)
                              CGACCATCGGGATCAGTAGAGTTCGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCT                                                                <  SRR4302533.1311534/94‑1 (MQ=60)
                               GACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTG                                                               >  SRR4302533.4773570/1‑94 (MQ=60)
                               GACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTG                                                               <  SRR4302533.201869/94‑1 (MQ=60)
                               GACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTG                                                               <  SRR4302533.5955934/94‑1 (MQ=60)
                                ACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGG                                                              >  SRR4302533.3025052/1‑94 (MQ=60)
                                ACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGG                                                              >  SRR4302533.4473860/1‑94 (MQ=60)
                                 CCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGC                                                             >  SRR4302533.5123809/1‑94 (MQ=60)
                                 CCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGC                                                             >  SRR4302533.2228027/1‑94 (MQ=60)
                                   ATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCAT                                                           <  SRR4302533.1154972/94‑1 (MQ=60)
                                    TCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATA                                                          <  SRR4302533.5485260/94‑1 (MQ=60)
                                      GGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGG                                                        <  SRR4302533.1122137/94‑1 (MQ=60)
                                      GGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGG                                                        >  SRR4302533.4121509/1‑94 (MQ=60)
                                      GGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGG                                                        >  SRR4302533.4964126/1‑94 (MQ=60)
                                      GGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGG                                                        <  SRR4302533.1477093/94‑1 (MQ=60)
                                       GGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGG                                                       >  SRR4302533.3799593/1‑94 (MQ=60)
                                        GATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGC                                                      <  SRR4302533.5812160/94‑1 (MQ=60)
                                        GATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGC                                                      <  SRR4302533.5984746/94‑1 (MQ=60)
                                         ATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCG                                                     >  SRR4302533.1450650/1‑94 (MQ=60)
                                         ATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCG                                                     >  SRR4302533.6297281/1‑94 (MQ=60)
                                         ATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGGTGGCTGGCATAGGGCG                                                     >  SRR4302533.3345043/1‑94 (MQ=60)
                                           CAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCT                                                   <  SRR4302533.4857872/94‑1 (MQ=60)
                                           CAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCT                                                   >  SRR4302533.5747036/1‑94 (MQ=60)
                                           CAGTAGAGTTTGCGCGCCAATAGCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCT                                                   <  SRR4302533.1495727/94‑1 (MQ=60)
                                            AGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTA                                                  <  SRR4302533.2209706/94‑1 (MQ=60)
                                            AGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTA                                                  >  SRR4302533.6035094/1‑94 (MQ=60)
                                            AGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTA                                                  <  SRR4302533.2804367/94‑1 (MQ=60)
                                            AGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTA                                                  >  SRR4302533.3227975/1‑94 (MQ=60)
                                              TAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACC                                                <  SRR4302533.3145244/94‑1 (MQ=60)
                                               AGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCG                                               <  SRR4302533.3216772/94‑1 (MQ=60)
                                               AGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCG                                               >  SRR4302533.6070893/1‑94 (MQ=60)
                                                 AGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCC                                             >  SRR4302533.3030187/1‑94 (MQ=60)
                                                 AGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCC                                             >  SRR4302533.4025999/1‑94 (MQ=60)
                                                   TTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTG                                           <  SRR4302533.3799115/94‑1 (MQ=60)
                                                    TTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGT                                          <  SRR4302533.3401647/94‑1 (MQ=60)
                                                      GCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAG                                        <  SRR4302533.4827502/94‑1 (MQ=60)
                                                        GCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCT                                      <  SRR4302533.188378/94‑1 (MQ=60)
                                                        GCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCT                                      <  SRR4302533.3929031/94‑1 (MQ=60)
                                                          GCCAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGA                                    >  SRR4302533.4061824/1‑94 (MQ=60)
                                                            CAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATG                                  >  SRR4302533.2393740/1‑94 (MQ=60)
                                                            CAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATG                                  <  SRR4302533.2744188/94‑1 (MQ=60)
                                                            CAATATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATG                                  >  SRR4302533.3431302/1‑94 (MQ=60)
                                                               TATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATA                               >  SRR4302533.6092569/1‑94 (MQ=60)
                                                               TATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATA                               >  SRR4302533.3340993/1‑94 (MQ=60)
                                                               TATCCAGGACTTGTTTAAACAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATA                               >  SRR4302533.880240/1‑94 (MQ=60)
                                                                ATCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAA                              <  SRR4302533.994056/94‑1 (MQ=60)
                                                                 TCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAA                             >  SRR4302533.407912/1‑94 (MQ=60)
                                                                 TCCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAA                             >  SRR4302533.585326/1‑94 (MQ=60)
                                                                  CCAGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAG                            >  SRR4302533.2338092/1‑94 (MQ=60)
                                                                    AGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGAT                          >  SRR4302533.1703418/1‑94 (MQ=60)
                                                                    AGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGAT                          <  SRR4302533.1800906/94‑1 (MQ=60)
                                                                    AGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGAT                          <  SRR4302533.100784/94‑1 (MQ=60)
                                                                    AGGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCCGGCATAGGGCGCTACTGCCTGTAGCTGATGATAAAGAT                          >  SRR4302533.3131635/1‑94 (MQ=60)
                                                                     GGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATC                         >  SRR4302533.5881628/1‑94 (MQ=60)
                                                                     GGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATC                         <  SRR4302533.5121975/94‑1 (MQ=60)
                                                                     GGACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATC                         >  SRR4302533.2445819/1‑94 (MQ=60)
                                                                      GACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCC                        <  SRR4302533.3893136/94‑1 (MQ=60)
                                                                       ACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCT                       >  SRR4302533.693104/1‑94 (MQ=60)
                                                                       ACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCT                       >  SRR4302533.1642548/1‑94 (MQ=60)
                                                                       ACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCT                       >  SRR4302533.4377379/1‑94 (MQ=60)
                                                                       ACTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCT                       >  SRR4302533.1925111/1‑94 (MQ=60)
                                                                        CTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTG                      >  SRR4302533.4114756/1‑94 (MQ=60)
                                                                        CTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTG                      >  SRR4302533.3708446/1‑94 (MQ=60)
                                                                        CTTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTG                      <  SRR4302533.1690139/94‑1 (MQ=60)
                                                                         TTGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGA                     >  SRR4302533.1794369/1‑94 (MQ=60)
                                                                          TGTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAA                    >  SRR4302533.3887600/1‑94 (MQ=60)
                                                                           GTTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAAT                   >  SRR4302533.4755079/1‑94 (MQ=60)
                                                                            TTTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATG                  >  SRR4302533.5272548/1‑94 (MQ=60)
                                                                             TTAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGG                 >  SRR4302533.1497508/1‑94 (MQ=60)
                                                                             TTAATCAGCGGTTTACCGCCTCCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGG                 <  SRR4302533.1306080/94‑1 (MQ=60)
                                                                              TAATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGT                >  SRR4302533.6189068/1‑94 (MQ=60)
                                                                               AATCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTG               <  SRR4302533.4543871/94‑1 (MQ=60)
                                                                                 TCAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTT             >  SRR4302533.2749520/1‑94 (MQ=60)
                                                                                  CAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATGCTGAATGGTGTTT            >  SRR4302533.3230393/1‑94 (MQ=60)
                                                                                  CAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTT            <  SRR4302533.2535750/94‑1 (MQ=60)
                                                                                  CAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTT            >  SRR4302533.2646991/1‑94 (MQ=60)
                                                                                  CAGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTT            >  SRR4302533.2323402/1‑94 (MQ=60)
                                                                                   AGCGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTG           <  SRR4302533.2275328/94‑1 (MQ=60)
                                                                                     CGGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGC         >  SRR4302533.564685/1‑94 (MQ=60)
                                                                                      GGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCG        <  SRR4302533.1684981/94‑1 (MQ=60)
                                                                                      GGTTTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCG        <  SRR4302533.2151130/94‑1 (MQ=60)
                                                                                         TTACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGT     >  SRR4302533.1693637/1‑94 (MQ=60)
                                                                                          TACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTG    >  SRR4302533.4499908/1‑94 (MQ=60)
                                                                                          TACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTG    >  SRR4302533.5101739/1‑94 (MQ=60)
                                                                                          TACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGACTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTG    >  SRR4302533.817658/1‑94 (MQ=60)
                                                                                           ACCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGC   >  SRR4302533.841187/1‑94 (MQ=60)
                                                                                            CCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTTGCGCGTGCT  >  SRR4302533.1249040/1‑94 (MQ=60)
                                                                                            CCGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGACTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCT  >  SRR4302533.5732811/1‑94 (MQ=60)
                                                                                             |                                                                                            
ACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCT  >  NC_000913/2356538‑2356723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑