Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,360,682 T→C A324A (GCA→GCG)  b2249 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,360,6820TC99.3% 437.7 / ‑6.4 142A324A (GCA→GCG) b2249hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (97/44);  total (97/45)
Fisher's exact test for biased strand distribution p-value = 3.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 210 total aligned reads displayed.

AGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACA  >  NC_000913/2360592‑2360771
                                                                                          |                                                                                         
aGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAgcgc                                                                                         >  2:4389643/1‑93 (MQ=255)
aGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAgcgc                                                                                         <  2:4990575/93‑1 (MQ=255)
 gTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAACGTTGAGATGCTCGTTCTCGAAACCCGAAACGGCCAGCGcc                                                                                        >  1:8184893/1‑93 (MQ=255)
 gTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGcc                                                                                        >  1:5920545/1‑93 (MQ=255)
 gTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGcc                                                                                        >  2:4351052/1‑93 (MQ=255)
 gTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGcc                                                                                        >  2:7055595/1‑93 (MQ=255)
  tGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCa                                                                                       >  1:6859942/1‑93 (MQ=255)
  tGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCa                                                                                       >  1:9706113/1‑93 (MQ=255)
   gCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAg                                                                                      <  1:1334406/93‑1 (MQ=255)
   gCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAg                                                                                      >  2:9608295/1‑93 (MQ=255)
   gCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAg                                                                                      >  2:1516168/1‑93 (MQ=255)
    cTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAGCCGGAAACAgccagcgccagc                                                                                     <  2:1627581/93‑1 (MQ=255)
    cTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAgccagcgccagc                                                                                     <  2:3400600/93‑1 (MQ=255)
     tGAAACGCGCACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGcc                                                                                    <  2:3876858/93‑1 (MQ=255)
      gAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGccc                                                                                   <  2:3646844/93‑1 (MQ=255)
      gAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGccc                                                                                   >  1:9550831/1‑93 (MQ=255)
       aaaCGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCg                                                                                  <  1:7740882/93‑1 (MQ=255)
        aaCGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCACCGCCAGCCCgg                                                                                 >  2:2791998/1‑93 (MQ=255)
        aaCGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTCGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCgg                                                                                 <  2:2484887/93‑1 (MQ=255)
          cGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCa                                                                               <  1:3023914/93‑1 (MQ=255)
            cacaCGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCaaa                                                                             >  2:7743349/1‑93 (MQ=255)
             acacGCAGAGCGAAATTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCaaaa                                                                            <  1:1828449/93‑1 (MQ=255)
             acacGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCaaaa                                                                            <  2:4019399/93‑1 (MQ=255)
             acacGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCaaaa                                                                            <  1:9121891/93‑1 (MQ=255)
             acacGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCaaaa                                                                            >  1:2608365/1‑93 (MQ=255)
             acacGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCACCCCGGCaaaa                                                                            >  1:7829162/1‑93 (MQ=255)
              cacGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAAt                                                                           <  2:3115502/93‑1 (MQ=255)
                  cAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGtt                                                                       <  2:3285232/93‑1 (MQ=255)
                  cAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGtt                                                                       <  2:6520901/93‑1 (MQ=255)
                   agagCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTg                                                                      >  1:5473505/1‑93 (MQ=255)
                   agagCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTg                                                                      >  1:4597244/1‑93 (MQ=255)
                    gagCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTgg                                                                     >  1:4665707/1‑93 (MQ=255)
                    gagCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTgg                                                                     >  2:9067769/1‑93 (MQ=255)
                    gagCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTgg                                                                     >  1:2120805/1‑93 (MQ=255)
                      gCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGcc                                                                   <  1:5793515/93‑1 (MQ=255)
                      gCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGcc                                                                   >  2:2722991/1‑93 (MQ=255)
                       cGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGccc                                                                  >  1:9369219/1‑93 (MQ=255)
                       cGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGACGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGccc                                                                  <  2:9234007/93‑1 (MQ=255)
                          aaGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCccgcc                                                               >  1:2231707/1‑93 (MQ=255)
                            gTGCGGTCTGGCGGGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGt                                                             <  2:7881171/93‑1 (MQ=255)
                            gTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGt                                                             <  2:5943085/93‑1 (MQ=255)
                            gTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGt                                                             >  2:8658060/1‑93 (MQ=255)
                             tGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGtt                                                            >  1:8710986/1‑93 (MQ=255)
                             ggCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGtt                                                            <  1:7621888/92‑1 (MQ=255)
                              gCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTc                                                           >  2:6119062/1‑93 (MQ=255)
                                 gTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGt                                                        >  1:8878754/1‑93 (MQ=255)
                                 gTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGt                                                        >  1:7647403/1‑93 (MQ=255)
                                 gTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGt                                                        >  1:5289429/1‑93 (MQ=255)
                                 gTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGt                                                        <  1:4247878/93‑1 (MQ=255)
                                   cgggCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTaa                                                      <  1:1783167/91‑1 (MQ=255)
                                   cTGGCGTGGCTAGCGCAAAGTTGATATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCGGGCAAAATGGTTGGCCCGCCGTTCTGTaa                                                      <  1:7933675/93‑1 (MQ=255)
                                    tGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAAt                                                     >  2:1432472/1‑93 (MQ=255)
                                     ggCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATc                                                    <  1:4447647/93‑1 (MQ=255)
                                      gCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATcc                                                   >  2:5663951/1‑93 (MQ=255)
                                      gCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATcc                                                   >  2:10006814/1‑93 (MQ=255)
                                       cGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCa                                                  <  2:814456/93‑1 (MQ=255)
                                         tGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGt                                                >  2:471789/1‑93 (MQ=255)
                                         tGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGt                                                >  2:7998761/1‑93 (MQ=255)
                                          ggCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTg                                               >  2:2193340/1‑93 (MQ=255)
                                          ggCTAGCCCAAAGTGGAGCTCCTCGTTCGCAAACCCCGAAACAGCCGGCGCTACCCCGGCAAATTGGTTGCCCCGCCGTTCTGTAATCCAGTg                                               <  2:5209919/93‑1 (MQ=255)
                                            cgaGCGCAAAGTGGAGATGCTCGTCCGCGAAACCCGAAACAGCCGGCACATGCCCGGTAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTgcg                                             <  2:6221285/91‑1 (MQ=255)
                                            cTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTgcg                                             >  2:10036354/1‑93 (MQ=255)
                                               gcgcAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAg                                          >  2:8876520/1‑93 (MQ=255)
                                                  cAAAGTGGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGttt                                       <  1:4130210/93‑1 (MQ=255)
                                                    aggTTGAGATGCTCGTTCTCGAACCCGAAAACAGCCAGCGCCAGCCCGGAAAAATGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTgc                                     <  2:7480085/91‑1 (MQ=255)
                                                        tGAGATGCTCGTTCTCGAACCCCGAAACACCCAGCCCCAGCCCGGCAAAATGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCa                                 <  1:6880333/93‑1 (MQ=255)
                                                         gagtTGCTGGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAg                                <  2:2578576/93‑1 (MQ=255)
                                                         gagaTGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAg                                >  2:3092977/1‑93 (MQ=255)
                                                         gagaTGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAg                                >  2:4593312/1‑93 (MQ=255)
                                                            aTGCTCGTTCTCGAACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGt                             <  1:6996942/93‑1 (MQ=255)
                                                              tctctTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGttt                           <  2:2853367/92‑1 (MQ=255)
                                                               cTCGTTCTCGAAGCCCGAAACAGACAGCGCCAGCCCGGCAAAATGGTGGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTtc                          <  2:6928788/93‑1 (MQ=255)
                                                               cTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTtc                          >  1:1933070/1‑93 (MQ=255)
                                                                tCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTtcc                         >  1:2395398/1‑93 (MQ=255)
                                                                 cGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTtcct                        >  1:9756291/1‑93 (MQ=255)
                                                                   ttCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTtcctcc                      >  1:862067/1‑93 (MQ=255)
                                                                    tctcAAACCCTGAATCAGCCGGCGCCAGCCGGCGAAAATGTTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTcctcct                     <  1:5151438/93‑1 (MQ=255)
                                                                      acGAAACCCAAAACAGCCCGCGCCGGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCtgt                   <  1:7489956/92‑1 (MQ=255)
                                                                       cGAACCCGGAACCAGCCAGCGCCAGCCGGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCtgtg                  <  2:1313831/93‑1 (MQ=255)
                                                                         aaaCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGaa                >  2:7146818/1‑93 (MQ=255)
                                                                         aaaCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGaa                >  1:6094791/1‑93 (MQ=255)
                                                                          aaCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAg               <  2:5392428/93‑1 (MQ=255)
                                                                          aaCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAg               >  1:10001565/1‑93 (MQ=255)
                                                                           aCCCAAACCAGCCAGCGCCGGCCGGGCAAAAGGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAgg              <  2:5621396/93‑1 (MQ=255)
                                                                             ccaaaaCAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGaa            <  1:7521396/90‑1 (MQ=255)
                                                                             ccGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGaa            >  2:1323230/1‑93 (MQ=255)
                                                                              caaaaCAGCCAGCGCCACCCCGGCAAAAGGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAc           <  2:4971495/91‑1 (MQ=255)
                                                                              caaaaCAGCCACCGCCAGCCCGGCAAAAGGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAc           <  2:9209493/91‑1 (MQ=255)
                                                                              cGACACAGCCAGCGCCAGCCGGGAAAAATGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAc           <  2:8589864/93‑1 (MQ=255)
                                                                               caaaCACCCGGCGCCAGCCCGGCAAAAGGGTGGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAcc          <  2:1401690/92‑1 (MQ=255)
                                                                                aaaCAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAccg         >  2:9882325/1‑92 (MQ=255)
                                                                                 aaCAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAccgc        >  1:4384330/1‑91 (MQ=255)
                                                                                 aaCAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAccgc        >  1:3297380/1‑91 (MQ=255)
                                                                                     gccagcgccagcCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTca    >  1:6462305/1‑93 (MQ=255)
                                                                                     gccagcgccagcCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTca    >  1:8294114/1‑93 (MQ=255)
                                                                                      ccAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTcac   >  2:1844968/1‑93 (MQ=255)
                                                                                      ccAGCGCCACCACGACAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTcac   <  1:5393827/93‑1 (MQ=255)
                                                                                       cAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTcaca  <  1:3081330/93‑1 (MQ=255)
                                                                                       cAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTcaca  >  1:1375087/1‑93 (MQ=255)
                                                                                       cAGCGCCAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTcaca  <  1:6570823/93‑1 (MQ=255)
                                                                                          |                                                                                         
AGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACA  >  NC_000913/2360592‑2360771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCC  >  NC_000913/2360590‑2360775
                                                                                            |                                                                                             
GTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCG                                                                                              <  SRR4302540.10021672/94‑1 (MQ=60)
GTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCG                                                                                              >  SRR4302540.1048410/1‑94 (MQ=60)
GTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCG                                                                                              >  SRR4302540.8900732/1‑94 (MQ=60)
GTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCG                                                                                              <  SRR4302540.1935428/94‑1 (MQ=60)
 TAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGC                                                                                             <  SRR4302540.8681564/94‑1 (MQ=60)
 GAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGC                                                                                             <  SRR4302540.2813164/94‑1 (MQ=60)
  AGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAACGTTGAGATGCTCGTTCTCGAAACCCGAAACGGCCAGCGCC                                                                                            >  SRR4302540.8279975/1‑94 (MQ=60)
  AGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCC                                                                                            >  SRR4302540.5985954/1‑94 (MQ=60)
  AGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCC                                                                                            >  SRR4302540.4667951/1‑94 (MQ=60)
   GTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCA                                                                                           >  SRR4302540.9820191/1‑94 (MQ=60)
   GTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCA                                                                                           >  SRR4302540.6935965/1‑94 (MQ=60)
    TGCCGAAACGCACAGGCAGAGCGAAAGTGCCGTCTCGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTTTCGAAACCCGAAACAGCCAGCGCCAG                                                                                          <  SRR4302540.9588550/94‑1 (MQ=60)
     GCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGC                                                                                         <  SRR4302540.1348368/94‑1 (MQ=60)
     GCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGC                                                                                         <  SRR4302540.1348369/94‑1 (MQ=60)
      CTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCC                                                                                        >  SRR4302540.7357422/1‑94 (MQ=60)
       TGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCC                                                                                       >  SRR4302540.9663805/1‑94 (MQ=60)
         AAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGG                                                                                     >  SRR4302540.7630502/1‑94 (MQ=60)
         AAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGG                                                                                     <  SRR4302540.7831254/94‑1 (MQ=60)
            CGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAA                                                                                  <  SRR4302540.3057136/94‑1 (MQ=60)
             GCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAA                                                                                 >  SRR4302540.5481246/1‑94 (MQ=60)
              CACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAA                                                                                >  SRR4302540.2636520/1‑94 (MQ=60)
              CACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCACCCCGGCAAAA                                                                                >  SRR4302540.7920647/1‑94 (MQ=60)
               ACACGCAGAGCGAAATTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAAT                                                                               <  SRR4302540.1848450/94‑1 (MQ=60)
               ACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAAT                                                                               <  SRR4302540.9231539/94‑1 (MQ=60)
                CACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATG                                                                              <  SRR4302540.7880566/94‑1 (MQ=60)
                 ACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGG                                                                             >  SRR4302540.8507510/1‑94 (MQ=60)
                  CGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGT                                                                            >  SRR4302540.10061777/1‑94 (MQ=60)
                    CAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTG                                                                          >  SRR4302540.4646678/1‑94 (MQ=60)
                    CAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTG                                                                          >  SRR4302540.5533752/1‑94 (MQ=60)
                     AGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGG                                                                         >  SRR4302540.4715651/1‑94 (MQ=60)
                     AGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGG                                                                         >  SRR4302540.2143560/1‑94 (MQ=60)
                        GCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCC                                                                      <  SRR4302540.5857530/94‑1 (MQ=60)
                        GCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCC                                                                      >  SRR4302540.9480770/1‑94 (MQ=60)
                         CGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAGCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCG                                                                     <  SRR4302540.4337879/94‑1 (MQ=60)
                         CGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCG                                                                     >  SRR4302540.8427490/1‑94 (MQ=60)
                          GAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGC                                                                    >  SRR4302540.2374429/1‑94 (MQ=60)
                           AAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCC                                                                   >  SRR4302540.2255734/1‑94 (MQ=60)
                           AAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCC                                                                   >  SRR4302540.5316144/1‑94 (MQ=60)
                              GTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTT                                                                >  SRR4302540.8812107/1‑94 (MQ=60)
                               GGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTC                                                               <  SRR4302540.7707603/94‑1 (MQ=60)
                                GCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCT                                                              >  SRR4302540.9697056/1‑94 (MQ=60)
                                GCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCT                                                              >  SRR4302540.7044146/1‑94 (MQ=60)
                                 CCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTG                                                             >  SRR4302540.3215036/1‑94 (MQ=60)
                                  CGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGT                                                            >  SRR4302540.5347243/1‑94 (MQ=60)
                                  CGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGT                                                            >  SRR4302540.8981500/1‑94 (MQ=60)
                                  CGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGT                                                            >  SRR4302540.2107994/1‑94 (MQ=60)
                                  CGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGT                                                            >  SRR4302540.7597289/1‑94 (MQ=60)
                                  CGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGT                                                            >  SRR4302540.7733487/1‑94 (MQ=60)
                                  CGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGT                                                            >  SRR4302540.7733488/1‑94 (MQ=60)
                                   GTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTA                                                           <  SRR4302540.4294093/94‑1 (MQ=60)
                                     CTGGCGTGGCTAGCGCAAAGTTGATATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCGGGCAAAATGGTTGGCCCGCCGTTCTGTAAT                                                         <  SRR4302540.8026145/94‑1 (MQ=60)
                                     CGGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAAT                                                         <  SRR4302540.1802540/94‑1 (MQ=60)
                                      TGGCGTGGCTAGCGAAAAGTTGAGGCGCGCGTTCTGGAACCCCGAAGCAGCCAGCGCCAGCCGGGCAAAATGGTTGGCCCGCCGTTCTGTAATC                                                        <  SRR4302540.890249/94‑1 (MQ=53)
                                       GGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCC                                                       <  SRR4302540.4495850/94‑1 (MQ=60)
                                          GTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGT                                                    >  SRR4302540.8289838/1‑94 (MQ=60)
                                          GTGGCTAGCGAAAAGTTGAGAGGCTCGTTCTCAAAACCCGAAACAGCCAGCGCCAGCCCGGAAAAATGTTTGGCCCGCCGTTCTGTAATCCAGT                                                    <  SRR4302540.8182005/94‑1 (MQ=60)
                                           TGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCCAAATGGTTGGCCCGCCGTTCTGTAATCCAGTG                                                   >  SRR4302540.8083628/1‑94 (MQ=60)
                                           GGGCTAGCGAAAAGTTGAGATGCTCGTTCTCAAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTG                                                   <  SRR4302540.4062596/94‑1 (MQ=60)
                                            GGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGC                                                  >  SRR4302540.5735133/1‑94 (MQ=60)
                                             GCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCTAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCG                                                 >  SRR4302540.1839255/1‑94 (MQ=60)
                                             GCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCG                                                 <  SRR4302540.7960572/94‑1 (MQ=60)
                                              CTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCAAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGC                                                >  SRR4302540.906036/1‑94 (MQ=60)
                                               TAGCGCAAAGTTGAGATGCTCGTTCTCGAACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCA                                               <  SRR4302540.6550319/94‑1 (MQ=60)
                                               NANCGCANANTTGAGATGCCNNNNCTCGAACCCCCAAACAGCCAGCCCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCA                                               <  SRR4302540.3075321/94‑1 (MQ=60)
                                                AGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAG                                              >  SRR4302540.4478427/1‑94 (MQ=60)
                                                    CAAAGTGGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTG                                          <  SRR4302540.4174860/94‑1 (MQ=60)
                                                     AAAGTTGAGATGCTGGTTCTCGAAACCGAAAACACCCAGCGCCAGCCCGGCAAAGTGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGC                                         <  SRR4302540.4373026/94‑1 (MQ=60)
                                                     AAAGTTGAGATGCTCGTTCTCGAACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGC                                         <  SRR4302540.6769567/94‑1 (MQ=60)
                                                      AAGTTGAGATGCTCGTTCTCGAAGCCGGAAACAGCCAGCGCCAGCCCGGCAAAAGGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCG                                        <  SRR4302540.5639235/94‑1 (MQ=60)
                                                      AAGTTGAGATGCTCGTTCTCGAACCCCAAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCG                                        <  SRR4302540.3114397/94‑1 (MQ=60)
                                                         TTGAGATGCTCGTTCTCGAACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCA                                     <  SRR4302540.8732751/94‑1 (MQ=60)
                                                          TGAGATGCTCGTTCTCGAACCCCGAAACACCCAGCCCCAGCCCGGCAAAATGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAG                                    <  SRR4302540.6956601/94‑1 (MQ=60)
                                                          TGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCGGCCCGGCAAAATGCTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAG                                    <  SRR4302540.6956599/94‑1 (MQ=60)
                                                              ATGCTCGTTCTCGAACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTT                                <  SRR4302540.7074602/94‑1 (MQ=60)
                                                                GCTCGTTCTCGAACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTC                              <  SRR4302540.187972/94‑1 (MQ=60)
                                                                GCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTC                              >  SRR4302540.1954135/1‑94 (MQ=60)
                                                                 gttgttctcaaacCCCGAAACAGCCAGCGCCAGCCCGGCAAAGGGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCC                             <  SRR4302540.3522737/81‑1 (MQ=60)
                                                                 CTCGTTCTCGAACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTGGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCC                             <  SRR4302540.622196/94‑1 (MQ=60)
                                                                 CTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCC                             >  SRR4302540.2421212/1‑94 (MQ=60)
                                                                 CTCGTTCACAAACCCCGAACCCGCCAGCGCCAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCC                             <  SRR4302540.7712585/94‑1 (MQ=60)
                                                                  TCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCT                            >  SRR4302540.9870748/1‑94 (MQ=60)
                                                                    GTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCC                          >  SRR4302540.871222/1‑94 (MQ=60)
                                                                    GTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCC                          >  SRR4302540.10161131/1‑94 (MQ=60)
                                                                     TTCTCGAAACCCAAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCT                         <  SRR4302540.262365/94‑1 (MQ=60)
                                                                       CTCGAACCCCGAAACAGCCAGCGCCAGCCCGGAAAAATGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGT                       <  SRR4302540.1789147/94‑1 (MQ=60)
                                                                       CTCGAACCCCAAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGT                       <  SRR4302540.6993532/94‑1 (MQ=60)
                                                                        ACGAAACCCAAAACAGCCCGCGCCGGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTG                      <  SRR4302540.7573575/94‑1 (MQ=60)
                                                                          GAACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAA                    <  SRR4302540.2868120/94‑1 (MQ=60)
                                                                          GAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAA                    >  SRR4302540.6162629/1‑94 (MQ=60)
                                                                           AACCCCAAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAG                   <  SRR4302540.2208210/94‑1 (MQ=60)
                                                                           AAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAG                   >  SRR4302540.10118077/1‑94 (MQ=60)
                                                                           AAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAG                   >  SRR4302540.4257485/1‑94 (MQ=60)
                                                                           AAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAG                   >  SRR4302540.9858831/1‑94 (MQ=60)
                                                                            ACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGG                  <  SRR4302540.4561/94‑1 (MQ=60)
                                                                             ACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGA                 >  SRR4302540.8833654/1‑94 (MQ=60)
                                                                              CCCGAAACAGCCACCCCCAGCCCGGCAAAAGGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAA                <  SRR4302540.6081682/94‑1 (MQ=60)
                                                                               CCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAC               >  SRR4302540.5265871/1‑94 (MQ=60)
                                                                               CCAAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAC               <  SRR4302540.7605522/94‑1 (MQ=60)
                                                                                CGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACC              >  SRR4302540.945854/1‑94 (MQ=60)
                                                                                CGAAACAGCCAGCCCCAGCCCGGCAAATTGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACC              <  SRR4302540.597281/94‑1 (MQ=60)
                                                                                  AAACAGCCAGCGCCCGCCCGGAAAAATGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGC            <  SRR4302540.2903738/94‑1 (MQ=60)
                                                                                  AAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGC            >  SRR4302540.4431930/1‑94 (MQ=60)
                                                                                  AAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGC            >  SRR4302540.3333469/1‑94 (MQ=60)
                                                                                      AGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCA        >  SRR4302540.6534226/1‑94 (MQ=60)
                                                                                      AGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCA        >  SRR4302540.8390429/1‑94 (MQ=60)
                                                                                        CCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACA      >  SRR4302540.5452072/1‑94 (MQ=60)
                                                                                        CCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACA      >  SRR4302540.1389677/1‑94 (MQ=60)
                                                                                         CAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACAC     <  SRR4302540.3115143/94‑1 (MQ=60)
                                                                                         CAGCGCCAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACAC     <  SRR4302540.6643627/94‑1 (MQ=60)
                                                                                         CAGCGCCACCCCGGAAAAATGTTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACAC     <  SRR4302540.3783523/94‑1 (MQ=60)
                                                                                         CAGCGCCACCCCGGAAAAATGGTCGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACAC     <  SRR4302540.499770/94‑1 (MQ=60)
                                                                                          AGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACG    >  SRR4302540.5807681/1‑94 (MQ=60)
                                                                                          AGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACG    >  SRR4302540.17400/1‑94 (MQ=60)
                                                                                          AGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACG    >  SRR4302540.3201063/1‑94 (MQ=60)
                                                                                            CGCCAGCCCGGAAAAAGGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCC  <  SRR4302540.5821698/94‑1 (MQ=60)
                                                                                            |                                                                                             
GTAGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCC  >  NC_000913/2360590‑2360775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑