Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,360,764 A→G V297A (GTG→GCG)  b2249 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,360,7640AG95.9% 718.0 / 10.8 242V297A (GTG→GCG) b2249hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (3/7);  new base G (114/118);  total (117/125)
Fisher's exact test for biased strand distribution p-value = 3.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 250 total aligned reads displayed.

GCCAGTGCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACTG  >  NC_000913/2360677‑2360855
                                                                                        |                                                                                           
gccagcgccagcCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTca                                                                                        >  1:8294114/1‑93 (MQ=255)
gccagcgccagcCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTca                                                                                        >  2:1173400/1‑93 (MQ=255)
  cAGCGCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTcaca                                                                                      >  1:5392774/1‑93 (MQ=255)
  cAGCGCCACCCCGGAAAAAT‑GTTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTcaca                                                                                      <  1:3742680/93‑1 (MQ=255)
  cAGCGCCACCCCGGAAAAAT‑GGTCGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTcaca                                                                                      <  1:494469/93‑1 (MQ=255)
    gcgCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACg                                                                                    >  1:3166630/3‑93 (MQ=255)
    gcgCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACg                                                                                    >  1:5744348/3‑93 (MQ=255)
     cgCCACCCGGGAAAAAT‑TTTCGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGc                                                                                   <  2:9571684/92‑1 (MQ=255)
      gCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGCACCGATTCACACgac                                                                                  >  2:5314269/1‑91 (MQ=255)
         cgCCGGGAAAAAT‑GTTGGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGc                                                                               <  2:714255/92‑1 (MQ=255)
         aGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGc                                                                               <  1:8021330/93‑1 (MQ=255)
           cccGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCaa                                                                             >  1:9435902/1‑93 (MQ=255)
            ccGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAAt                                                                            >  2:1031960/1‑93 (MQ=255)
              gggaaaaTGGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATg                                                                           <  1:17232/90‑1 (MQ=255)
              ggaaaaaT‑GTTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATgg                                                                          <  1:6577546/90‑1 (MQ=255)
               gCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGa                                                                         <  2:6191601/93‑1 (MQ=255)
                cAAAAT‑GGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAg                                                                        <  1:1690269/93‑1 (MQ=255)
                 aaaaT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCGATGGAGc                                                                       >  2:4223308/1‑93 (MQ=255)
                 aaaaT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAga                                                                       >  2:3665654/1‑92 (MQ=255)
                  aaaT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCa                                                                      >  1:6798576/1‑93 (MQ=255)
                   agt‑GGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCAc                                                                     <  1:6550736/91‑1 (MQ=255)
                   aaT‑GGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCAc                                                                     <  1:9530437/93‑1 (MQ=255)
                     t‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCt                                                                   <  1:1894913/93‑1 (MQ=255)
                     t‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCt                                                                   >  2:7503233/1‑93 (MQ=255)
                            ccccGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCga                                                             <  2:6929792/92‑1 (MQ=255)
                             cccGCCTTTCTGTAATCCATTGCGCAGGTTGCGCCAGTGTTTCCTCCTGTGGAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCgag                                                            <  1:6391153/93‑1 (MQ=255)
                             cccGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCgag                                                            >  2:6322592/1‑93 (MQ=255)
                               cgccgTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGaa                                                          <  1:3477804/93‑1 (MQ=255)
                               cgccgTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGaa                                                          >  2:609528/1‑93 (MQ=255)
                               cgccgTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGaa                                                          <  2:4597243/93‑1 (MQ=255)
                                gccgTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGaaa                                                         >  2:5738252/1‑93 (MQ=255)
                                 ccgTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAg                                                        >  2:389437/1‑93 (MQ=255)
                                 ccgTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAg                                                        >  2:167206/1‑93 (MQ=255)
                                 ccgTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAg                                                        >  2:9134696/1‑93 (MQ=255)
                                   gTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGtt                                                      >  2:3447485/1‑93 (MQ=255)
                                    ttCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTg                                                     <  1:6834101/93‑1 (MQ=255)
                                    ttCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTg                                                     <  2:8493077/93‑1 (MQ=255)
                                    ttCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTg                                                     >  1:2149764/1‑93 (MQ=255)
                                     tCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGc                                                    <  2:3258953/93‑1 (MQ=255)
                                       tGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCaa                                                  >  1:7918969/1‑93 (MQ=255)
                                       tGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCaa                                                  >  2:526856/1‑93 (MQ=255)
                                       tGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCaa                                                  <  2:2329823/93‑1 (MQ=255)
                                        gTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCaaa                                                 >  2:9039241/1‑93 (MQ=255)
                                         tAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACATGCGCGAGAAAGTTGCAAAg                                                <  2:562773/93‑1 (MQ=255)
                                           aTCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGcc                                              <  2:5473504/93‑1 (MQ=255)
                                           aTCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGcc                                              <  1:1022051/93‑1 (MQ=255)
                                            tCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCa                                             <  2:8724921/93‑1 (MQ=255)
                                            tCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCa                                             >  2:951947/1‑93 (MQ=255)
                                             cgaGTGCGCAGGTTGCGCCAGGGTTTCCTCCTGTGAAGTAACCGCTTCGCACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCaa                                            <  1:5122481/91‑1 (MQ=255)
                                             ccAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCaa                                            <  2:5928511/93‑1 (MQ=255)
                                                gTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATaa                                         >  1:8847733/1‑93 (MQ=255)
                                                gTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATaa                                         >  1:9661199/1‑93 (MQ=255)
                                                 tGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATaaa                                        <  2:3985633/93‑1 (MQ=255)
                                                 tGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATaaa                                        >  1:7557565/1‑93 (MQ=255)
                                                  gcgcAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAac                                       <  2:6636333/93‑1 (MQ=255)
                                                   cgcAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAacc                                      <  2:9355785/93‑1 (MQ=255)
                                                   cgcAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAacc                                      >  1:3775683/1‑93 (MQ=255)
                                                     cAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAaccac                                    >  1:9288525/1‑93 (MQ=255)
                                                      aGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCCCCTGCGCGAGAAAGTTGCAAAGCCAATAAaccacc                                   <  1:2838822/93‑1 (MQ=255)
                                                       gTTTGCGCCAGGGTTTCCTCCTGTGAAGTCACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCg                                  <  1:2668709/93‑1 (MQ=255)
                                                       gTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCg                                  >  1:4344282/1‑93 (MQ=255)
                                                        tttGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCgg                                 >  2:7281573/1‑93 (MQ=255)
                                                         ttGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGt                                <  2:7021029/93‑1 (MQ=255)
                                                            cgcCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGaa                             <  2:3552249/93‑1 (MQ=255)
                                                             gcCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAAt                            <  2:6507586/93‑1 (MQ=255)
                                                              ccAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAAtt                           <  2:8773886/93‑1 (MQ=255)
                                                              ccAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAAtt                           >  1:4552167/1‑93 (MQ=255)
                                                               cAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTg                          <  2:8330790/93‑1 (MQ=255)
                                                               cAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTg                          >  2:4710410/1‑93 (MQ=255)
                                                                 gggTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTgct                        <  2:626520/93‑1 (MQ=255)
                                                                 gggTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTgct                        >  2:7062479/1‑93 (MQ=255)
                                                                  ggTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTgctc                       <  1:5865998/93‑1 (MQ=255)
                                                                  ggTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTgctc                       >  2:2436786/1‑93 (MQ=255)
                                                                  ggTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTgctc                       <  2:1710238/93‑1 (MQ=255)
                                                                   gTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTgctcg                      >  1:6213543/1‑93 (MQ=255)
                                                                   gTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTgctcg                      <  1:4406239/93‑1 (MQ=255)
                                                                    tttCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTgctcgc                     >  2:1581716/1‑93 (MQ=255)
                                                                      tcctccTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTgctcgctc                   >  2:4783024/1‑93 (MQ=255)
                                                                       cctcctGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCa                  >  1:1504252/1‑93 (MQ=255)
                                                                        ctcctGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCTGTGAATTGCTCGCTCaa                 <  1:1242181/93‑1 (MQ=255)
                                                                        ctcctGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCaa                 >  1:9874301/1‑93 (MQ=255)
                                                                         tcctGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAAc                >  2:4415326/1‑93 (MQ=255)
                                                                         tcctGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAAc                >  1:9085157/1‑93 (MQ=255)
                                                                          cctGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACg               <  2:9369218/93‑1 (MQ=255)
                                                                          cctGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACg               <  1:7743350/93‑1 (MQ=255)
                                                                           ctGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGt              <  1:8557775/93‑1 (MQ=255)
                                                                            tgtgAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTc             <  2:3194326/93‑1 (MQ=255)
                                                                            tgtgAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTc             <  1:733139/93‑1 (MQ=255)
                                                                            tgtgAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTc             >  1:8051675/1‑93 (MQ=255)
                                                                            tgtgAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTc             >  2:1482095/1‑93 (MQ=255)
                                                                            tgtgAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTc             <  2:304640/93‑1 (MQ=255)
                                                                             gtgAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCa            >  1:918761/1‑93 (MQ=255)
                                                                             gtgAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCa            <  1:5661752/93‑1 (MQ=255)
                                                                                aaGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGc         <  2:2633624/93‑1 (MQ=255)
                                                                                aaGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGc         >  1:5958132/1‑93 (MQ=255)
                                                                                 aGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCt        <  1:4621183/93‑1 (MQ=255)
                                                                                    aaCCACTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGaa     <  2:7726307/93‑1 (MQ=255)
                                                                                    aaCCACTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGaa     <  2:198517/93‑1 (MQ=255)
                                                                                     aCCGCTTCACACGCCAGCAATGGAGCAGCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAAc    <  1:4825677/93‑1 (MQ=255)
                                                                                     aCCACTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAAc    <  1:3962552/93‑1 (MQ=255)
                                                                                        |                                                                                           
GCCAGTGCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACTG  >  NC_000913/2360677‑2360855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACAGCCAGTGCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACTGG  >  NC_000913/2360672‑2360856
                                                                                             |                                                                                            
aaacgcccggcgcaAGCCCGGAAAAGT‑GGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCAC                                                                                             <  SRR4302540.5571411/80‑1 (MQ=60)
AAACAGCCAGCGCCCGCCCGGAAAAAT‑GGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGC                                                                                             <  SRR4302540.2903738/94‑1 (MQ=60)
AAACAGCCAGCGCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGC                                                                                             >  SRR4302540.3333469/1‑94 (MQ=60)
AAACAGCCAGCGCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGC                                                                                             >  SRR4302540.4431930/1‑94 (MQ=60)
  acccccgccgccgGCCCGGCAAAGT‑GGTGGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTT                                                                                           <  SRR4302540.1052636/81‑1 (MQ=60)
   cgtgccggcacaaccccgccaaattgttcgcCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGTTTTCCTCCTGTGAAGGAACCGCTT                                                                                           <  SRR4302540.1052635/63‑1 (MQ=60)
    AGCCAGCGCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCA                                                                                         >  SRR4302540.6534226/1‑94 (MQ=60)
    AGCCAGCGCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCA                                                                                         >  SRR4302540.8390429/1‑94 (MQ=60)
      ccatccCCAGCCCGGAAAAAT‑GGTCGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACA                                                                                       <  SRR4302540.5453439/88‑1 (MQ=60)
      ccagcgccaccacgaCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACA                                                                                       <  SRR4302540.5453133/79‑1 (MQ=60)
      CCAGCGCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACA                                                                                       >  SRR4302540.1389677/1‑94 (MQ=60)
      CCAGCGCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACA                                                                                       >  SRR4302540.5452072/1‑94 (MQ=60)
       cagccccacCCCGGAAAAAG‑GGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACAC                                                                                      <  SRR4302540.8368443/85‑1 (MQ=60)
       CAGCGCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACAC                                                                                      <  SRR4302540.3115143/94‑1 (MQ=60)
       CAGCGCCAGCCCGGAAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACAC                                                                                      <  SRR4302540.6643627/94‑1 (MQ=60)
       CAGCGCCACCCCGGAAAAAT‑GTTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACAC                                                                                      <  SRR4302540.3783523/94‑1 (MQ=60)
       CAGCGCCACCCCGGAAAAAT‑GGTCGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACAC                                                                                      <  SRR4302540.499770/94‑1 (MQ=60)
        AGCGCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACG                                                                                     >  SRR4302540.3201063/1‑94 (MQ=60)
        AGCGCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACG                                                                                     >  SRR4302540.5807681/1‑94 (MQ=60)
        AGCGCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACG                                                                                     >  SRR4302540.17400/1‑94 (MQ=60)
          CGCCAGCCCGGAAAAAG‑GGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCC                                                                                   <  SRR4302540.5821698/94‑1 (MQ=60)
           GCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCA                                                                                  >  SRR4302540.9690003/1‑94 (MQ=60)
            caaccccggaaaagT‑GGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAG                                                                                 <  SRR4302540.2717917/80‑1 (MQ=60)
             CAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGC                                                                                >  SRR4302540.5664333/1‑94 (MQ=60)
              AGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCA                                                                               <  SRR4302540.8114623/94‑1 (MQ=60)
               GCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAA                                                                              >  SRR4302540.9547966/1‑94 (MQ=60)
               GCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAA                                                                              >  SRR4302540.9687278/1‑94 (MQ=60)
                CCCGGAAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAAT                                                                             <  SRR4302540.7833798/94‑1 (MQ=60)
                 cgcgacaaaagggtggcCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAAT                                                                             <  SRR4302540.4165673/77‑1 (MQ=60)
                  cggaaaaaatgtgtgcCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATG                                                                            <  SRR4302540.7645088/78‑1 (MQ=60)
                  CGGCAAAAT‑GGTGGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGG                                                                           <  SRR4302540.7007532/94‑1 (MQ=60)
                  CGGCAAAAG‑GGTTTGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGGTTCACACGCCAGCAATGG                                                                           <  SRR4302540.17422/94‑1 (MQ=60)
                   GGGAAAATGGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGG                                                                           <  SRR4302540.17420/94‑1 (MQ=60)
                   GGCAAATT‑GGTGGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGA                                                                          <  SRR4302540.7807093/94‑1 (MQ=60)
                   GGAAAAAT‑GTTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGA                                                                          <  SRR4302540.6650399/94‑1 (MQ=60)
                   GCCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGA                                                                          <  SRR4302540.5174132/94‑1 (MQ=60)
                     CAAAAT‑GGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGC                                                                        <  SRR4302540.1708601/94‑1 (MQ=60)
                      AAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCA                                                                       >  SRR4302540.6873782/1‑94 (MQ=60)
                       AAAT‑GGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCAC                                                                      <  SRR4302540.5967964/94‑1 (MQ=60)
                        AGT‑GGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACC                                                                     <  SRR4302540.6623395/94‑1 (MQ=60)
                        AAT‑GGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACC                                                                     <  SRR4302540.9643267/94‑1 (MQ=60)
                        AAT‑GGTGGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACC                                                                     <  SRR4302540.6871524/94‑1 (MQ=60)
                         atgGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACC                                                                     <  SRR4302540.4619312/91‑1 (MQ=60)
                         AT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCT                                                                    <  SRR4302540.8497047/94‑1 (MQ=60)
                          T‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTG                                                                   <  SRR4302540.1915651/94‑1 (MQ=60)
                          T‑GGTGGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTG                                                                   <  SRR4302540.8060206/94‑1 (MQ=60)
                                  CCCGCCTTTCTGTAATCCATTGCGCAGGTTGCGCCAGTGTTTCCTCCTGTGGAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGA                                                            <  SRR4302540.6462297/94‑1 (MQ=60)
                                  CCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGA                                                            <  SRR4302540.6462296/94‑1 (MQ=60)
                                    CGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAA                                                          <  SRR4302540.3515672/94‑1 (MQ=60)
                                        GTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTG                                                      >  SRR4302540.8187058/1‑94 (MQ=60)
                                        GTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTG                                                      >  SRR4302540.2172882/1‑94 (MQ=60)
                                         TTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGC                                                     <  SRR4302540.6909791/94‑1 (MQ=60)
                                         TTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGC                                                     >  SRR4302540.4449432/1‑94 (MQ=60)
                                           CTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAA                                                   <  SRR4302540.9512530/94‑1 (MQ=60)
                                           CTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAA                                                   >  SRR4302540.8011274/1‑94 (MQ=60)
                                             GTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAG                                                 >  SRR4302540.6484023/1‑94 (MQ=60)
                                               AATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCC                                               >  SRR4302540.1940323/1‑94 (MQ=60)
                                                ATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCA                                              <  SRR4302540.7497915/94‑1 (MQ=60)
                                                ATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCA                                              <  SRR4302540.3275908/94‑1 (MQ=60)
                                                ATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCA                                              <  SRR4302540.1032421/94‑1 (MQ=60)
                                                  CGAGTGCGCAGGTTGCGCCAGGGTTTCCTCCTGTGAAGTAACCGCTTCGCACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAAT                                            <  SRR4302540.5177944/94‑1 (MQ=60)
                                                  CCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAAT                                            <  SRR4302540.5177942/94‑1 (MQ=60)
                                                  CCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAAT                                            >  SRR4302540.6863112/1‑94 (MQ=60)
                                                  CCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAAT                                            >  SRR4302540.9579456/1‑94 (MQ=60)
                                                   CAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATA                                           >  SRR4302540.3453847/1‑94 (MQ=60)
                                                    AGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAA                                          >  SRR4302540.3668847/1‑94 (MQ=60)
                                                    AGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAA                                          >  SRR4302540.8053940/1‑94 (MQ=60)
                                                    AGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAA                                          >  SRR4302540.9774922/1‑94 (MQ=60)
                                                    AGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAA                                          >  SRR4302540.8950083/1‑94 (MQ=60)
                                                    AGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAA                                          >  SRR4302540.6908661/1‑94 (MQ=60)
                                                     GTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAA                                         >  SRR4302540.7642315/1‑94 (MQ=60)
                                                       GCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACC                                       >  SRR4302540.3816875/1‑94 (MQ=60)
                                                        CGCAGTTTGCGCCAGGGTTTCTTCCTGTGAAGGAGCCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCA                                      <  SRR4302540.6038229/94‑1 (MQ=60)
                                                         GCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCAC                                     >  SRR4302540.9399530/1‑94 (MQ=60)
                                                         GCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCAC                                     >  SRR4302540.4809239/1‑94 (MQ=60)
                                                          CAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACC                                    <  SRR4302540.9212868/94‑1 (MQ=60)
                                                           AGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCCCCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCG                                   <  SRR4302540.2869526/94‑1 (MQ=60)
                                                           AGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCG                                   <  SRR4302540.2453566/94‑1 (MQ=60)
                                                           AGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCG                                   >  SRR4302540.4391566/1‑94 (MQ=60)
                                                            GTTTGCGCCAGGGTTTCCTCCTGTGAAGTCACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGG                                  <  SRR4302540.2697626/94‑1 (MQ=60)
                                                            GTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGG                                  <  SRR4302540.437779/94‑1 (MQ=60)
                                                             GTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGT                                 <  SRR4302540.4776560/94‑1 (MQ=60)
                                                              TTGCGCCAGGGTTTCCTACTGTGAAGGAACCACTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTG                                <  SRR4302540.5265698/94‑1 (MQ=60)
                                                                 CGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAAT                             <  SRR4302540.8114898/94‑1 (MQ=60)
                                                                 CGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAAT                             <  SRR4302540.7668429/94‑1 (MQ=60)
                                                                 CGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAAT                             <  SRR4302540.3790013/94‑1 (MQ=60)
                                                                  GCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATT                            >  SRR4302540.9314342/1‑94 (MQ=60)
                                                                  GCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATT                            >  SRR4302540.4601393/1‑94 (MQ=60)
                                                                   CCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTG                           >  SRR4302540.7140679/1‑94 (MQ=60)
                                                                       GGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCG                       <  SRR4302540.5930851/94‑1 (MQ=60)
                                                                       GGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCG                       >  SRR4302540.6282679/1‑94 (MQ=60)
                                                                       GGTGTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCG                       <  SRR4302540.4957417/94‑1 (MQ=60)
                                                                        GTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGC                      <  SRR4302540.1729973/94‑1 (MQ=60)
                                                                        GTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGC                      <  SRR4302540.4454007/94‑1 (MQ=60)
                                                                         TTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCT                     <  SRR4302540.9504013/94‑1 (MQ=60)
                                                                         TTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCT                     >  SRR4302540.3902813/1‑94 (MQ=60)
                                                                           TCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCA                   >  SRR4302540.1520844/1‑94 (MQ=60)
                                                                            CCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAA                  >  SRR4302540.9989614/1‑94 (MQ=60)
                                                                            CCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAA                  >  SRR4302540.452220/1‑94 (MQ=60)
                                                                             CTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCTGTGAATTGCTCGCTCAAC                 <  SRR4302540.1254854/94‑1 (MQ=60)
                                                                             CTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAAC                 >  SRR4302540.9194310/1‑94 (MQ=60)
                                                                              TCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACG                <  SRR4302540.379189/94‑1 (MQ=60)
                                                                               CCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGT               <  SRR4302540.7833758/94‑1 (MQ=60)
                                                                               CCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGT               >  SRR4302540.8728464/1‑94 (MQ=60)
                                                                               CCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGT               <  SRR4302540.5910383/94‑1 (MQ=60)
                                                                                CTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTC              >  SRR4302540.8145189/1‑94 (MQ=60)
                                                                                CTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTC              >  SRR4302540.4385017/1‑94 (MQ=60)
                                                                                CTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTC              <  SRR4302540.8657175/94‑1 (MQ=60)
                                                                                CTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTC              >  SRR4302540.1224088/1‑94 (MQ=60)
                                                                                 TGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCA             >  SRR4302540.6266264/1‑94 (MQ=60)
                                                                                 TGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCA             <  SRR4302540.740977/94‑1 (MQ=60)
                                                                                 TGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCA             >  SRR4302540.928456/1‑94 (MQ=60)
                                                                                 TGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCA             >  SRR4302540.928455/1‑94 (MQ=60)
                                                                                  GTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAG            <  SRR4302540.8212625/94‑1 (MQ=60)
                                                                                  GTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAG            <  SRR4302540.5805423/94‑1 (MQ=60)
                                                                                  GTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAG            <  SRR4302540.5724097/94‑1 (MQ=60)
                                                                                   TGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGG           >  SRR4302540.2054822/1‑94 (MQ=60)
                                                                                   TGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGG           >  SRR4302540.1599916/1‑94 (MQ=60)
                                                                                    GAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGC          >  SRR4302540.6024031/1‑94 (MQ=60)
                                                                                      AGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTG        <  SRR4302540.4670719/94‑1 (MQ=60)
                                                                                       GGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGA       <  SRR4302540.2752349/94‑1 (MQ=60)
                                                                                         AACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAAC     >  SRR4302540.8948442/1‑94 (MQ=60)
                                                                                         AACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAAC     <  SRR4302540.4595824/94‑1 (MQ=60)
                                                                                         AACCGCTTCACACGCAAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAAC     <  SRR4302540.9067893/94‑1 (MQ=60)
                                                                                          ACCGCTTCACACGCCAGCAATGGAGCAGCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACT    <  SRR4302540.4877357/94‑1 (MQ=60)
                                                                                          ACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACT    >  SRR4302540.1184257/1‑94 (MQ=60)
                                                                                          ACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACT    >  SRR4302540.6318743/1‑94 (MQ=60)
                                                                                          ACCACTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACT    <  SRR4302540.4005283/94‑1 (MQ=60)
                                                                                           CCGCTTCACACTCCAGCAAGGGAGCACCTGCGCGATAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACTG   <  SRR4302540.764784/94‑1 (MQ=60)
                                                                                           CCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACTG   <  SRR4302540.9660191/94‑1 (MQ=60)
                                                                                           CCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACTG   <  SRR4302540.2787739/94‑1 (MQ=60)
                                                                                           CCACTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACTG   >  SRR4302540.9869275/1‑94 (MQ=60)
                                                                                            CGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAGACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACTGG  <  SRR4302540.8758633/94‑1 (MQ=60)
                                                                                            CGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACTGG  >  SRR4302540.5749516/1‑94 (MQ=60)
                                                                                            CGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACTGG  <  SRR4302540.8758634/94‑1 (MQ=60)
                                                                                            CGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACTGG  >  SRR4302540.1056133/1‑94 (MQ=60)
                                                                                             |                                                                                            
AAACAGCCAGTGCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACTGG  >  NC_000913/2360672‑2360856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑