Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,362,769 G→A E66K (GAA→AAA)  yfaO → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,362,7690GA99.0% 1502.8 / ‑1.9 415E66K (GAA→AAA) yfaOhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/4);  new base A (205/203);  total (208/207)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 431 total aligned reads displayed.

TCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATT  >  NC_000913/2362679‑2362858
                                                                                          |                                                                                         
tCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACaaaa                                                                                         <  2:2268135/93‑1 (MQ=255)
tCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACaaaa                                                                                         >  2:5023590/1‑93 (MQ=255)
 cGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAaaaa                                                                                        <  2:6793766/93‑1 (MQ=255)
  gggTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAAt                                                                                       <  2:7614003/93‑1 (MQ=255)
   ggTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATc                                                                                      <  2:6231680/93‑1 (MQ=255)
    gTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCa                                                                                     <  1:5298134/93‑1 (MQ=255)
    gTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCa                                                                                     <  2:8539892/93‑1 (MQ=255)
      ggcggcGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACg                                                                                   <  2:2695748/93‑1 (MQ=255)
      ggcggcGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACg                                                                                   <  2:9438745/93‑1 (MQ=255)
      ggcggcGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACg                                                                                   <  1:1841040/93‑1 (MQ=255)
      ggcggcGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACg                                                                                   >  1:3708135/1‑93 (MQ=255)
       gcggcgTGGAGCCTGGCGAACGAATGGAAGAGGCACTCCGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGc                                                                                  <  2:5463472/93‑1 (MQ=255)
       gcggcgTGGAGCCTCGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGc                                                                                  <  1:2787108/93‑1 (MQ=255)
        cggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGcc                                                                                 >  2:2903287/1‑93 (MQ=255)
         ggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCg                                                                                <  1:7428409/93‑1 (MQ=255)
          gcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGt                                                                               <  1:8263852/93‑1 (MQ=255)
           cgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTg                                                                              >  1:9018688/1‑93 (MQ=255)
            gTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTgg                                                                             >  1:10012310/1‑93 (MQ=255)
            gTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTgg                                                                             >  1:9921465/1‑93 (MQ=255)
             tGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGa                                                                            <  1:5078979/93‑1 (MQ=255)
             tGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGa                                                                            >  2:7955364/1‑93 (MQ=255)
             tGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGa                                                                            >  2:7222919/1‑93 (MQ=255)
              ggAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAc                                                                           <  1:1195025/93‑1 (MQ=255)
                aGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCt                                                                         <  2:8895890/93‑1 (MQ=255)
                   cTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACACAAATCACGCCGTGGACCTTCa                                                                      >  2:4561385/1‑93 (MQ=255)
                   cTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCa                                                                      >  1:4710033/1‑93 (MQ=255)
                   cTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCa                                                                      >  2:2360865/1‑93 (MQ=255)
                    tGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAg                                                                     >  1:1588033/1‑93 (MQ=255)
                    tGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAg                                                                     >  1:4099394/1‑93 (MQ=255)
                    tGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAg                                                                     >  1:6232304/1‑93 (MQ=255)
                     ggCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGc                                                                    >  2:9662325/1‑93 (MQ=255)
                     ggCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGc                                                                    >  1:4938550/1‑93 (MQ=255)
                      gCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCg                                                                   <  2:7343167/93‑1 (MQ=255)
                      gCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCg                                                                   >  2:3908928/1‑93 (MQ=255)
                      gCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCg                                                                   >  1:9403915/1‑93 (MQ=255)
                       cgaacgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCga                                                                  >  1:6154500/1‑93 (MQ=255)
                        gaacgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCgat                                                                 <  2:1897349/93‑1 (MQ=255)
                        gaacgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCgat                                                                 >  2:8361835/1‑93 (MQ=255)
                         aacgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCgatg                                                                >  2:1395093/1‑93 (MQ=255)
                         aacgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCgatg                                                                >  1:9188912/1‑93 (MQ=255)
                          acgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCgatga                                                               <  2:9778230/93‑1 (MQ=255)
                          acgaaTTGAAGAGGCACTACGCCGCGAAAGTCGTGAAGAACGGGGAGAACAGCTGCGTTTGACAAAAATCACGCCGTGGACCTTCAGCgatga                                                               <  1:1924615/93‑1 (MQ=255)
                               ttGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTc                                                          >  1:2046951/1‑93 (MQ=255)
                                tGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTca                                                         >  1:3109007/1‑92 (MQ=255)
                                tGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCg                                                         >  2:6980612/1‑93 (MQ=255)
                                 gAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGc                                                        <  2:4743478/93‑1 (MQ=255)
                                 gAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGc                                                        <  2:2194073/93‑1 (MQ=255)
                                   agagGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCAc                                                      <  1:6606138/93‑1 (MQ=255)
                                    gagGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCAcc                                                     <  1:2546243/93‑1 (MQ=255)
                                      ggCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCaa                                                   <  2:772635/93‑1 (MQ=255)
                                       gCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAg                                                  <  1:4507294/93‑1 (MQ=255)
                                       gCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAg                                                  <  2:448355/93‑1 (MQ=255)
                                        cACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCACGa                                                 >  2:10011233/1‑93 (MQ=255)
                                         aCTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGAc                                                >  1:6101286/1‑93 (MQ=255)
                                           tACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGt                                              >  2:9910520/1‑93 (MQ=255)
                                            aCGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTa                                             <  2:7530724/93‑1 (MQ=255)
                                            aCGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTa                                             <  2:4916800/93‑1 (MQ=255)
                                             cgccgaGAAATTGGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTAt                                            <  2:6592807/93‑1 (MQ=255)
                                               ccgcGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGc                                          >  2:2049393/1‑93 (MQ=255)
                                               ccgcGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGc                                          <  1:2342747/93‑1 (MQ=255)
                                                   gAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAt                                      >  2:6007958/1‑93 (MQ=255)
                                                    aaaTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATg                                     <  2:1094107/93‑1 (MQ=255)
                                                    aaaTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTAGCACCAAGACGTATGCAGATg                                     >  1:719539/1‑93 (MQ=255)
                                                      aTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGt                                   <  1:3391287/93‑1 (MQ=255)
                                                      aTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGt                                   <  1:7956807/93‑1 (MQ=255)
                                                       ttCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTc                                  <  1:6989343/93‑1 (MQ=255)
                                                           cgAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCaa                              <  1:6817264/93‑1 (MQ=255)
                                                              agaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGa                           <  1:1048703/93‑1 (MQ=255)
                                                               gaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGAACCAAGACGTATGCAGATGGTCGCAAGGaa                          >  2:9168120/1‑93 (MQ=255)
                                                                  cTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGAATGCAGATGTCCGCAACGAagat                       >  1:2897240/1‑92 (MQ=255)
                                                                    gggAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAt                     <  1:220389/93‑1 (MQ=255)
                                                                    gggAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAt                     >  2:5671655/1‑93 (MQ=255)
                                                                     ggAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAtt                    <  2:5278555/93‑1 (MQ=255)
                                                                     ggAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAtt                    <  2:3630571/93‑1 (MQ=255)
                                                                       agaACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTta                  >  1:9314562/1‑93 (MQ=255)
                                                                       agaACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTta                  <  1:9563784/93‑1 (MQ=255)
                                                                        gaACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtat                 >  2:2580605/1‑93 (MQ=255)
                                                                        gaACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtat                 >  1:3210655/1‑93 (MQ=255)
                                                                        gaACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtat                 >  2:6422496/1‑93 (MQ=255)
                                                                         aaCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtata                <  1:8155424/93‑1 (MQ=255)
                                                                         aaCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtata                <  1:9866354/93‑1 (MQ=255)
                                                                         aaCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtata                >  2:3124422/1‑93 (MQ=255)
                                                                           cAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATg              >  2:3199673/1‑93 (MQ=255)
                                                                             gctgctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAt            >  1:4688874/1‑93 (MQ=255)
                                                                              ctgctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAtt           >  1:8582380/1‑93 (MQ=255)
                                                                              ctgctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAtt           >  2:1216474/1‑93 (MQ=255)
                                                                              ctgctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAtt           >  2:2605725/1‑93 (MQ=255)
                                                                              ctgctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAtt           <  1:586690/93‑1 (MQ=255)
                                                                                  ttttGACAAAAATCACGCCTTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTAcc       <  1:9781321/93‑1 (MQ=255)
                                                                                   tttGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCt      <  2:8209050/93‑1 (MQ=255)
                                                                                   tttGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCt      >  2:4154419/1‑93 (MQ=255)
                                                                                   tttGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCt      >  2:6893246/1‑93 (MQ=255)
                                                                                   tttGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCt      >  2:3463013/1‑93 (MQ=255)
                                                                                   tttGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCt      >  1:8668842/1‑93 (MQ=255)
                                                                                    ttGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCGCCAAGACGTATGCAGATGGTCGC‑AGGAAGAGATTTATATGATTTACCTGa    <  2:806728/93‑1 (MQ=255)
                                                                                     tGACAAAAATCGCGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGa    <  1:7725156/93‑1 (MQ=255)
                                                                                     tGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGa    >  1:6463274/1‑93 (MQ=255)
                                                                                     tGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTACATGATTTACCTGa    >  1:5206486/1‑93 (MQ=255)
                                                                                      gACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAt   >  1:8451091/1‑93 (MQ=255)
                                                                                       aCAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAtt  >  1:560086/1‑93 (MQ=255)
                                                                                          |                                                                                         
TCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATT  >  NC_000913/2362679‑2362858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 226 total aligned reads displayed.
ATTTCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTTG  >  NC_000913/2362676‑2362862
                                                                                             |                                                                                             
ATTTCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTCCGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAA                                                                                               >  SRR4302540.8006922/1‑94 (MQ=60)
ATTTCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAA                                                                                               >  SRR4302540.3766803/1‑94 (MQ=60)
  TTCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAA                                                                                             <  SRR4302540.10102581/94‑1 (MQ=60)
     GGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATC                                                                                          >  SRR4302540.8329314/1‑94 (MQ=60)
     GGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATC                                                                                          >  SRR4302540.6328123/1‑94 (MQ=60)
     GGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATC                                                                                          >  SRR4302540.6328124/1‑94 (MQ=60)
      GGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCA                                                                                         >  SRR4302540.9990482/1‑94 (MQ=60)
      GGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCA                                                                                         >  SRR4302540.9906922/1‑94 (MQ=60)
       GTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCAC                                                                                        <  SRR4302540.6020964/94‑1 (MQ=60)
       GTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCAC                                                                                        <  SRR4302540.5356094/94‑1 (MQ=60)
        TGGCGGCGTGGGGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACG                                                                                       <  SRR4302540.2273594/94‑1 (MQ=60)
        TGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACG                                                                                       >  SRR4302540.3748603/1‑94 (MQ=60)
        TGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACG                                                                                       <  SRR4302540.7928210/94‑1 (MQ=60)
        TGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACG                                                                                       <  SRR4302540.6575667/94‑1 (MQ=60)
        TGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACG                                                                                       <  SRR4302540.6575665/94‑1 (MQ=60)
        GGGCGGGGTGGAGCCGGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACG                                                                                       <  SRR4302540.1885495/94‑1 (MQ=60)
         GGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGC                                                                                      <  SRR4302540.1861209/94‑1 (MQ=60)
          GCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCC                                                                                     <  SRR4302540.2817209/94‑1 (MQ=60)
          GCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCC                                                                                     <  SRR4302540.8546746/94‑1 (MQ=60)
          GCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCC                                                                                     <  SRR4302540.2975270/94‑1 (MQ=60)
          GCGGCGTGGAGCCTCGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCC                                                                                     <  SRR4302540.2817208/94‑1 (MQ=60)
           CGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCG                                                                                    >  SRR4302540.8932703/1‑94 (MQ=60)
           CGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCG                                                                                    <  SRR4302540.9426409/94‑1 (MQ=60)
            GGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGT                                                                                   >  SRR4302540.2485520/1‑94 (MQ=60)
            GGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGT                                                                                   <  SRR4302540.7511162/94‑1 (MQ=60)
             GCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTG                                                                                  >  SRR4302540.9126906/1‑94 (MQ=60)
             GCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTG                                                                                  <  SRR4302540.8359879/94‑1 (MQ=60)
             GCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTG                                                                                  <  SRR4302540.10161398/94‑1 (MQ=60)
              CGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGG                                                                                 >  SRR4302540.10037320/1‑94 (MQ=60)
              CGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGG                                                                                 >  SRR4302540.10128886/1‑94 (MQ=60)
                TGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAC                                                                               <  SRR4302540.5133890/94‑1 (MQ=60)
                TGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAC                                                                               >  SRR4302540.1778267/1‑94 (MQ=60)
                TGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAC                                                                               <  SRR4302540.1720002/94‑1 (MQ=60)
                GGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAC                                                                               <  SRR4302540.7436716/94‑1 (MQ=60)
                 GGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACC                                                                              <  SRR4302540.1207331/94‑1 (MQ=60)
                  GAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCT                                                                             <  SRR4302540.6863085/94‑1 (MQ=60)
                   AGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTT                                                                            <  SRR4302540.7919908/94‑1 (MQ=60)
                   AGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTT                                                                            <  SRR4302540.5184573/94‑1 (MQ=60)
                    GCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTC                                                                           <  SRR4302540.6216593/94‑1 (MQ=60)
                     CCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCA                                                                          >  SRR4302540.4760372/1‑94 (MQ=60)
                     CCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCA                                                                          >  SRR4302540.4760371/1‑94 (MQ=60)
                      CTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAG                                                                         >  SRR4302540.4143617/1‑94 (MQ=60)
                      CTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAG                                                                         >  SRR4302540.4143618/1‑94 (MQ=60)
                      CTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAG                                                                         >  SRR4302540.1605383/1‑94 (MQ=60)
                      CTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAG                                                                         >  SRR4302540.6301619/1‑94 (MQ=60)
                      CTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAG                                                                         >  SRR4302540.7284118/1‑94 (MQ=60)
                      CTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAG                                                                         >  SRR4302540.10138831/1‑94 (MQ=60)
                       TGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGCACTGGGAGCACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGC                                                                        >  SRR4302540.4991564/1‑94 (MQ=60)
                       TGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGC                                                                        >  SRR4302540.534417/1‑94 (MQ=60)
                       TGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGC                                                                        >  SRR4302540.6364888/1‑94 (MQ=60)
                       TGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGC                                                                        >  SRR4302540.4991843/1‑94 (MQ=60)
                        GGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCG                                                                       <  SRR4302540.8981041/94‑1 (MQ=60)
                         GCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGA                                                                      >  SRR4302540.6223062/1‑94 (MQ=60)
                          CGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGAT                                                                     >  SRR4302540.9567376/1‑94 (MQ=60)
                           GAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATG                                                                    >  SRR4302540.9299107/1‑94 (MQ=60)
                           GAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATG                                                                    <  SRR4302540.8473053/94‑1 (MQ=60)
                           GAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATG                                                                    >  SRR4302540.671251/1‑94 (MQ=60)
                           GAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATG                                                                    >  SRR4302540.9403440/1‑94 (MQ=60)
                           GAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATG                                                                    <  SRR4302540.8473054/94‑1 (MQ=60)
                            AACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGA                                                                   >  SRR4302540.3358490/1‑94 (MQ=60)
                             ACGAATTGAAGAGGCACTACGCCGCGAAAGTCGTGAAGAACGGGGAGAACAGCTGCGTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGAT                                                                  <  SRR4302540.1945596/94‑1 (MQ=60)
                               GAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATAT                                                                <  SRR4302540.6182414/94‑1 (MQ=60)
                               GAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATAT                                                                <  SRR4302540.8717368/94‑1 (MQ=60)
                               GAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATAT                                                                >  SRR4302540.266259/1‑94 (MQ=60)
                               GAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATAT                                                                <  SRR4302540.3016946/94‑1 (MQ=60)
                                AATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATT                                                               >  SRR4302540.8714732/1‑94 (MQ=60)
                                AATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATT                                                               >  SRR4302540.8714731/1‑94 (MQ=60)
                                 ATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGGTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTC                                                              >  SRR4302540.1425465/1‑94 (MQ=60)
                                 ATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTC                                                              <  SRR4302540.3599620/94‑1 (MQ=60)
                                 ATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTC                                                              >  SRR4302540.2647844/1‑94 (MQ=60)
                                 ATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTC                                                              >  SRR4302540.2069004/1‑94 (MQ=60)
                                 ATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTC                                                              >  SRR4302540.2770625/1‑94 (MQ=60)
                                  TTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCG                                                             >  SRR4302540.6869261/1‑94 (MQ=60)
                                  TTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCG                                                             >  SRR4302540.4181387/1‑94 (MQ=60)
                                  TTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCA                                                             >  SRR4302540.3143029/1‑94 (MQ=60)
                                  ATGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCG                                                             <  SRR4302540.5289261/94‑1 (MQ=60)
                                   TGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGC                                                            >  SRR4302540.5346269/1‑94 (MQ=60)
                                   TGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGC                                                            >  SRR4302540.8812114/1‑94 (MQ=60)
                                    GAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCA                                                           <  SRR4302540.10108741/94‑1 (MQ=60)
                                      AGAGGTACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACC                                                         >  SRR4302540.5425905/1‑94 (MQ=60)
                                      AGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACC                                                         >  SRR4302540.9464317/1‑94 (MQ=60)
                                      AGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACC                                                         <  SRR4302540.6679288/94‑1 (MQ=60)
                                       GAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCA                                                        >  SRR4302540.9663620/1‑94 (MQ=60)
                                       GAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCA                                                        <  SRR4302540.9982833/94‑1 (MQ=60)
                                       GAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCA                                                        <  SRR4302540.2573688/94‑1 (MQ=60)
                                       GAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCA                                                        >  SRR4302540.4898117/1‑94 (MQ=60)
                                        AGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAA                                                       >  SRR4302540.4341832/1‑94 (MQ=60)
                                         GGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAG                                                      >  SRR4302540.1373741/1‑94 (MQ=60)
                                         GGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAG                                                      <  SRR4302540.4900912/94‑1 (MQ=60)
                                          GCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACACAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGA                                                     >  SRR4302540.4675786/1‑94 (MQ=60)
                                          GCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGA                                                     >  SRR4302540.6991988/1‑94 (MQ=60)
                                          GCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGA                                                     <  SRR4302540.4556140/94‑1 (MQ=60)
                                           CACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACAAAGAC                                                    >  SRR4302540.5066071/1‑94 (MQ=60)
                                            ACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACG                                                   >  SRR4302540.4776578/1‑94 (MQ=60)
                                            ACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACG                                                   >  SRR4302540.306641/1‑94 (MQ=60)
                                             CTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGT                                                  >  SRR4302540.7231192/1‑94 (MQ=60)
                                             CTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGT                                                  >  SRR4302540.3173960/1‑94 (MQ=60)
                                              TACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTA                                                 >  SRR4302540.656670/1‑94 (MQ=60)
                                               ACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTAT                                                <  SRR4302540.9648221/94‑1 (MQ=60)
                                                CGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATG                                               <  SRR4302540.9485078/94‑1 (MQ=60)
                                                 GCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGC                                              >  SRR4302540.5072492/1‑94 (MQ=60)
                                                 GCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGC                                              >  SRR4302540.8108117/1‑94 (MQ=60)
                                                 GCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGC                                              <  SRR4302540.5675942/94‑1 (MQ=60)
                                                  CCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTcagcggttgtatttccaacaaagacgaatgcg                                             >  SRR4302540.9818604/1‑62 (MQ=60)
                                                  CCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCA                                             >  SRR4302540.7995741/1‑94 (MQ=60)
                                                  CCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCA                                             <  SRR4302540.2367988/94‑1 (MQ=60)
                                                     CGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAT                                          <  SRR4302540.9400531/94‑1 (MQ=60)
                                                     CGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAT                                          >  SRR4302540.1003555/1‑94 (MQ=60)
                                                     CGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAT                                          <  SRR4302540.3404839/94‑1 (MQ=60)
                                                     CGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAT                                          <  SRR4302540.1131818/94‑1 (MQ=60)
                                                      GAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATG                                         <  SRR4302540.4741853/94‑1 (MQ=60)
                                                      GAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATG                                         >  SRR4302540.5522754/1‑94 (MQ=60)
                                                      GAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTAGCACCAAGACGTATGCAGATG                                         >  SRR4302540.727256/1‑94 (MQ=60)
                                                       AAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGG                                        >  SRR4302540.6066389/1‑94 (MQ=60)
                                                        AATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGT                                       >  SRR4302540.3942244/1‑94 (MQ=60)
                                                         ATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTC                                      <  SRR4302540.3428285/94‑1 (MQ=60)
                                                         ATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTC                                      <  SRR4302540.8049554/94‑1 (MQ=60)
                                                         ATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTC                                      <  SRR4302540.8339666/94‑1 (MQ=60)
                                                         ATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTC                                      <  SRR4302540.2106909/94‑1 (MQ=60)
                                                         ATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTC                                      <  SRR4302540.2035338/94‑1 (MQ=60)
                                                         ATTCGCGAAGAACGGGGAGAACAGCTGCGGTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTC                                      <  SRR4302540.9779075/94‑1 (MQ=60)
                                                          TTCGCGAAGAACTGGGAGAGCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCG                                     <  SRR4302540.9898119/94‑1 (MQ=60)
                                                          TTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCG                                     <  SRR4302540.7066932/94‑1 (MQ=60)
                                                             GCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAA                                  <  SRR4302540.6175848/94‑1 (MQ=60)
                                                              CGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAG                                 <  SRR4302540.6892723/94‑1 (MQ=60)
                                                               GAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGG                                <  SRR4302540.9698012/94‑1 (MQ=60)
                                                               GAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGG                                >  SRR4302540.2315435/1‑94 (MQ=60)
                                                                AAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGA                               <  SRR4302540.3531063/94‑1 (MQ=60)
                                                                AAGAACTGGGAAAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGA                               <  SRR4302540.2763066/94‑1 (MQ=60)
                                                                 AGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAA                              <  SRR4302540.1059368/94‑1 (MQ=60)
                                                                 AGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAA                              <  SRR4302540.1059367/94‑1 (MQ=60)
                                                                    ACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAG                           >  SRR4302540.9627701/1‑94 (MQ=60)
                                                                    ACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGAATGCAGATGtccgcaacgaagat                           >  SRR4302540.2928842/1‑80 (MQ=60)
                                                                     CTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGA                          <  SRR4302540.171094/94‑1 (MQ=60)
                                                                     CTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGA                          >  SRR4302540.6244834/1‑94 (MQ=60)
                                                                      TGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAT                         >  SRR4302540.10017469/1‑94 (MQ=60)
                                                                       GGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATT                        <  SRR4302540.222531/94‑1 (MQ=60)
                                                                       GGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATT                        >  SRR4302540.3248055/1‑94 (MQ=60)
                                                                       GGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATT                        <  SRR4302540.7259957/94‑1 (MQ=60)
                                                                        GGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTT                       <  SRR4302540.1882501/94‑1 (MQ=60)
                                                                        GGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTT                       <  SRR4302540.6117173/94‑1 (MQ=60)
                                                                        GGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTT                       >  SRR4302540.4738078/1‑94 (MQ=60)
                                                                        GGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTT                       >  SRR4302540.3903453/1‑94 (MQ=60)
                                                                         GAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTA                      <  SRR4302540.9324711/94‑1 (MQ=60)
                                                                         GAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTA                      >  SRR4302540.9425731/1‑94 (MQ=60)
                                                                          AGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTAT                     <  SRR4302540.9676831/94‑1 (MQ=60)
                                                                          AGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTAT                     >  SRR4302540.3245509/1‑94 (MQ=60)
                                                                           GAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAATACGTATGCAGATGGTCGCAAGGAAGAGATTTATA                    >  SRR4302540.2160709/1‑94 (MQ=60)
                                                                           GAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATA                    >  SRR4302540.10072398/1‑94 (MQ=60)
                                                                           GAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATA                    >  SRR4302540.2187305/1‑94 (MQ=60)
                                                                            AACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATAT                   >  SRR4302540.6253103/1‑94 (MQ=60)
                                                                            AACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATAT                   <  SRR4302540.9981605/94‑1 (MQ=60)
                                                                            AACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATAT                   >  SRR4302540.9920019/1‑94 (MQ=60)
                                                                            AACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATAT                   <  SRR4302540.8250114/94‑1 (MQ=60)
                                                                            AACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATAT                   <  SRR4302540.5184012/94‑1 (MQ=60)
                                                                            AACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATAT                   <  SRR4302540.7159296/94‑1 (MQ=60)
                                                                             ACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATG                  >  SRR4302540.4561994/1‑94 (MQ=60)
                                                                               AGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAT                >  SRR4302540.4739019/1‑94 (MQ=60)
                                                                               AGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAT                >  SRR4302540.2472849/1‑94 (MQ=60)
                                                                                GCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATT               <  SRR4302540.2034284/94‑1 (MQ=60)
                                                                                GCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATT               <  SRR4302540.7733676/94‑1 (MQ=60)
                                                                                GCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATT               <  SRR4302540.6915640/94‑1 (MQ=60)
                                                                                GCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATT               >  SRR4302540.8682149/1‑94 (MQ=60)
                                                                                GCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATT               <  SRR4302540.7768361/94‑1 (MQ=60)
                                                                                 CTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTT              <  SRR4302540.593021/94‑1 (MQ=60)
                                                                                 CTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTT              >  SRR4302540.7091983/1‑94 (MQ=60)
                                                                                  TGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTA             <  SRR4302540.8050349/94‑1 (MQ=60)
                                                                                  TGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTA             <  SRR4302540.5626275/94‑1 (MQ=60)
                                                                                  TGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTA             >  SRR4302540.2062346/1‑94 (MQ=60)
                                                                                  TGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTA             >  SRR4302540.314525/1‑94 (MQ=60)
                                                                                   GCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTAC            >  SRR4302540.2260584/1‑94 (MQ=60)
                                                                                   GCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTAC            <  SRR4302540.8067118/94‑1 (MQ=60)
                                                                                    CTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACT           >  SRR4302540.1156883/1‑94 (MQ=60)
                                                                                    CTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACC           <  SRR4302540.5220379/94‑1 (MQ=60)
                                                                                     TTTTGACAAAAATCACGCCTTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCT          <  SRR4302540.9895954/94‑1 (MQ=60)
                                                                                     TTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCT          <  SRR4302540.9244438/94‑1 (MQ=60)
                                                                                     TTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCT          >  SRR4302540.8769537/1‑94 (MQ=60)
                                                                                      TTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTG         >  SRR4302540.8370416/1‑94 (MQ=60)
                                                                                      TTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTG         <  SRR4302540.602466/94‑1 (MQ=60)
                                                                                       TTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGA        <  SRR4302540.3166406/94‑1 (MQ=60)
                                                                                       TTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGA        >  SRR4302540.3848697/1‑94 (MQ=60)
                                                                                       TTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGA        >  SRR4302540.6535206/1‑94 (MQ=60)
                                                                                       TTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTACATGATTTACCTGA        >  SRR4302540.5263200/1‑94 (MQ=60)
                                                                                        TGACAAAAATCGCGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAT       <  SRR4302540.7815269/94‑1 (MQ=60)
                                                                                        TGACAAAAATCACGCCGTGGCCCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAT       >  SRR4302540.5826084/1‑94 (MQ=60)
                                                                                        TGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAT       >  SRR4302540.8616271/1‑94 (MQ=60)
                                                                                        TGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAT       <  SRR4302540.1810654/94‑1 (MQ=60)
                                                                                        TGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAT       >  SRR4302540.8549206/1‑94 (MQ=60)
                                                                                        TGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAT       >  SRR4302540.8616272/1‑94 (MQ=60)
                                                                                         GACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTTGCAAGGAAGAGATTTATATGATTTACCTGATT      <  SRR4302540.9200895/94‑1 (MQ=60)
                                                                                         GACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATT      >  SRR4302540.566180/1‑94 (MQ=60)
                                                                                          ACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTT     <  SRR4302540.755193/94‑1 (MQ=60)
                                                                                          ACAAAAACCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTT     <  SRR4302540.2864273/94‑1 (MQ=60)
                                                                                            AAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCTCCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTT   <  SRR4302540.6213565/94‑1 (MQ=60)
                                                                                            AAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTT   <  SRR4302540.5564747/94‑1 (MQ=60)
                                                                                            AAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTT   >  SRR4302540.8918668/1‑94 (MQ=60)
                                                                                            AAAAAGCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTT   <  SRR4302540.6213566/94‑1 (MQ=60)
                                                                                             AAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTTG  <  SRR4302540.8860761/94‑1 (MQ=60)
                                                                                             AAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTTG  <  SRR4302540.7082203/94‑1 (MQ=60)
                                                                                             AAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTTG  >  SRR4302540.432760/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATTTCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATTTTTG  >  NC_000913/2362676‑2362862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑