Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,370,228 A→G H434R (CAT→CGT)  b2257 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,370,2280AG100.0% 655.9 / NA 206H434R (CAT→CGT) b2257hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (97/109);  total (97/109)

Only 100 of 212 total aligned reads displayed.

AAAAGACCTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACATCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGG  >  NC_000913/2370138‑2370317
                                                                                          |                                                                                          
  aaGACCTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCg                                                                                        <  2:4249348/93‑1 (MQ=255)
  aaGACCTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCg                                                                                        >  1:955467/1‑93 (MQ=255)
    gACCTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCa                                                                                      >  2:892360/1‑93 (MQ=255)
     aCCTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCaa                                                                                     <  1:1387827/93‑1 (MQ=255)
      ccTGGCCTTTAGCCGCGCTTTGCCCGCTGGGACTGGCGTTGCAGGTAGAATTTTCAATTCCTGACAGAGTTAAGGAAGGAAAAAGTCCGCAA‑t                                                                                   <  1:6307438/93‑1 (MQ=255)
       cTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑tt                                                                                  >  2:1431227/1‑93 (MQ=255)
        tGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑ttt                                                                                 >  1:1086313/1‑93 (MQ=255)
        tGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑ttt                                                                                 >  1:5688976/1‑93 (MQ=255)
         ggCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑tttt                                                                                >  2:451931/1‑93 (MQ=255)
            cTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTg                                                                             >  2:4001007/1‑93 (MQ=255)
               ttGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCg                                                                          <  2:1874100/93‑1 (MQ=255)
               ttGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCg                                                                          <  2:1124975/93‑1 (MQ=255)
               ttGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCg                                                                          <  2:1563631/93‑1 (MQ=255)
               ttGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCg                                                                          <  1:3108220/93‑1 (MQ=255)
                tGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCga                                                                         <  1:925344/93‑1 (MQ=255)
                tGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCga                                                                         <  1:531402/93‑1 (MQ=255)
                  ccGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCgaga                                                                       <  1:2565571/93‑1 (MQ=255)
                  ccGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCgaga                                                                       <  2:237426/93‑1 (MQ=255)
                    gcgcTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATg                                                                     >  1:554773/1‑93 (MQ=255)
                    gcgcTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATg                                                                     >  1:3019742/1‑93 (MQ=255)
                    ccgcTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATg                                                                     <  2:6248938/92‑1 (MQ=255)
                       cTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGaca                                                                  <  1:1207508/93‑1 (MQ=255)
                       cTTTGCCCGCTGGGGCTGGCGGTGCTGGTAGGATTTTCAATTCCTGACAGAGGTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGaca                                                                  >  2:3833931/1‑93 (MQ=255)
                        tttGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGacac                                                                 <  1:5993388/93‑1 (MQ=255)
                           gCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAg                                                              >  1:3282785/1‑93 (MQ=255)
                           gCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAg                                                              >  2:3450713/1‑93 (MQ=255)
                           gCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAg                                                              <  2:2289222/93‑1 (MQ=255)
                            cccGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGa                                                             <  2:6038669/93‑1 (MQ=255)
                             ccGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGaa                                                            <  1:1781461/93‑1 (MQ=255)
                             ccGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGaa                                                            <  1:4081943/93‑1 (MQ=255)
                               gCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATc                                                          <  2:678870/93‑1 (MQ=255)
                                 tGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGGGATGACACAAGAATCAc                                                        >  2:4916078/1‑93 (MQ=255)
                                 tGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCAc                                                        <  1:2630497/93‑1 (MQ=255)
                                 tGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCAc                                                        >  1:5222018/1‑93 (MQ=255)
                                  ggggCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTTGTCGAGATGACACAAGAATCAc                                                        <  2:3552318/93‑1 (MQ=255)
                                  ggggCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACt                                                       >  1:959076/1‑93 (MQ=255)
                                   gggCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACtt                                                      <  2:1304132/93‑1 (MQ=255)
                                   gggCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAACGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACtt                                                      >  2:5854063/1‑93 (MQ=255)
                                    ggCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTc                                                     <  2:4363415/93‑1 (MQ=255)
                                    ggCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTc                                                     <  1:6196095/93‑1 (MQ=255)
                                     gCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCa                                                    <  2:5362013/93‑1 (MQ=255)
                                     gCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCa                                                    <  1:3871120/93‑1 (MQ=255)
                                     gCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCa                                                    <  2:117200/93‑1 (MQ=255)
                                     gCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCa                                                    <  2:4414277/93‑1 (MQ=255)
                                     gCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCa                                                    <  2:1574013/93‑1 (MQ=255)
                                      cTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAg                                                   >  2:2192465/1‑93 (MQ=255)
                                      cTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAg                                                   <  1:5865396/93‑1 (MQ=255)
                                         gCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCa                                                <  1:1005010/93‑1 (MQ=255)
                                         gCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCa                                                <  1:2453193/93‑1 (MQ=255)
                                          ggtggCTGGTAGGATTTTAAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCaa                                               <  2:546894/89‑1 (MQ=255)
                                           gTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAg                                              <  1:2869343/93‑1 (MQ=255)
                                             tGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGcc                                            >  1:1145789/1‑93 (MQ=255)
                                               cTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGc                                          >  1:4303961/1‑93 (MQ=255)
                                                 ggTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCta                                        >  1:999973/1‑93 (MQ=255)
                                                 ggTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCta                                        <  2:3950851/93‑1 (MQ=255)
                                                 ggTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCta                                        <  2:4182527/93‑1 (MQ=255)
                                                  gTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAT‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCtat                                       <  2:227091/93‑1 (MQ=255)
                                                  gTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCtat                                       >  1:4579959/1‑93 (MQ=255)
                                                  gTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCtat                                       >  2:6172531/1‑93 (MQ=255)
                                                   tAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCtata                                      >  1:4467315/1‑93 (MQ=255)
                                                   tAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCtata                                      >  1:3214684/1‑93 (MQ=255)
                                                   tAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCtata                                      >  2:2785829/1‑93 (MQ=255)
                                                        ttttCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCtt                                 >  2:205937/1‑93 (MQ=255)
                                                         tttCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTa                                <  2:2023171/93‑1 (MQ=255)
                                                           tCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACt                              <  1:1532418/93‑1 (MQ=255)
                                                              aTTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGAt                           >  2:810987/1‑93 (MQ=255)
                                                              aTTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGAt                           >  1:2049091/1‑93 (MQ=255)
                                                              aTTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGAt                           <  2:4938149/93‑1 (MQ=255)
                                                               ttCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATa                          <  2:4603983/93‑1 (MQ=255)
                                                               ttCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATa                          <  1:4723215/93‑1 (MQ=255)
                                                                tCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAg                         >  1:2154617/1‑93 (MQ=255)
                                                                tCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAg                         <  2:6082929/93‑1 (MQ=255)
                                                                tCCTGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAg                         >  2:5046737/1‑93 (MQ=255)
                                                                 ccTGACAGATTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGc                        <  2:243112/93‑1 (MQ=255)
                                                                   tGACAGAGTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGt                      >  2:308623/1‑93 (MQ=255)
                                                                   cgACAAACTTATGGAAGCAAACCGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGt                      <  2:4681936/92‑1 (MQ=255)
                                                                    gACAGAGTGATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTc                     <  1:3191234/93‑1 (MQ=255)
                                                                      cAGAGTTATGGAAGTAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCgg                   <  1:2005676/93‑1 (MQ=255)
                                                                       agagTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGgt                  >  1:1500322/1‑93 (MQ=255)
                                                                       agagTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGgt                  >  1:4426759/1‑93 (MQ=255)
                                                                        gagTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGgtg                 <  2:6033012/93‑1 (MQ=255)
                                                                        gagTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGgtg                 >  2:1496485/1‑93 (MQ=255)
                                                                        gagTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGgtg                 >  1:2286572/1‑93 (MQ=255)
                                                                         agTTATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGgtgt                <  2:3218828/93‑1 (MQ=255)
                                                                            tATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGc             <  1:1849118/93‑1 (MQ=255)
                                                                            tATGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGc             >  1:498711/1‑93 (MQ=255)
                                                                              tGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCg           >  2:563254/1‑93 (MQ=255)
                                                                              tGGAAGGAAAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCg           >  1:697903/1‑93 (MQ=255)
                                                                               ggaaggaaAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGc          >  1:2801203/1‑93 (MQ=255)
                                                                               ggaaggaaAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGc          <  1:4787265/93‑1 (MQ=255)
                                                                                gaaggaaAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCa         >  2:3718757/1‑93 (MQ=255)
                                                                                gaaggaaAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCa         >  1:57488/1‑93 (MQ=255)
                                                                                 aaggaaAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAg        >  2:3678412/1‑93 (MQ=255)
                                                                                 aaggaaAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAg        <  1:4848351/93‑1 (MQ=255)
                                                                                 aaggaaAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAg        <  1:776405/93‑1 (MQ=255)
                                                                                  aggaaAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAgg       >  1:5075870/1‑93 (MQ=255)
                                                                                   ggaaAACGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGt      <  1:1957660/93‑1 (MQ=255)
                                                                                     aaaaCGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCt    >  2:5213742/1‑93 (MQ=255)
                                                                                       aaCGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTgg  <  2:1962309/93‑1 (MQ=255)
                                                                                       aaCGTCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTgg  >  2:3324298/1‑93 (MQ=255)
                                                                                          |                                                                                          
AAAAGACCTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACATCCGCAA‑TTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGG  >  NC_000913/2370138‑2370317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCGAAAAGACCTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACATCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATG  >  NC_000913/2370135‑2370321
                                                                                             |                                                                                             
TCGAAAAGACCTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACG                                                                                               >  SRR4302533.5410219/1‑94 (MQ=60)
  GAAAAGACCTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTC                                                                                             <  SRR4302533.2644575/94‑1 (MQ=60)
   AAAAGACCTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCC                                                                                            <  SRR4302533.4365122/94‑1 (MQ=60)
    AAAGACCTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCG                                                                                           >  SRR4302533.961075/1‑94 (MQ=60)
    AAAGACCTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCG                                                                                           <  SRR4302533.4972499/94‑1 (MQ=60)
      AGACCTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCA                                                                                         >  SRR4302533.6032652/1‑94 (MQ=60)
        ACCTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAAT                                                                                       <  SRR4302533.1396183/94‑1 (MQ=60)
        ACCTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAAT                                                                                       <  SRR4302533.817648/94‑1 (MQ=60)
         CCTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATT                                                                                      >  SRR4302533.1960697/1‑94 (MQ=60)
         CCTGGCCTTTAGCCGCGCTTTGCCCGCTGGGACTGGCGTTGCAGGTAGAATTTTCAATTCCTGACAGAGTTAAGGAAGGAAAAAGTCCGCAATT                                                                                      <  SRR4302533.6345252/94‑1 (MQ=60)
          CTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTT                                                                                     >  SRR4302533.5723338/1‑94 (MQ=60)
          CTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTT                                                                                     >  SRR4302533.1092716/1‑94 (MQ=60)
            GGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTT                                                                                   >  SRR4302533.2086356/1‑94 (MQ=60)
            GGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTT                                                                                   <  SRR4302533.5701367/94‑1 (MQ=60)
              CCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTG                                                                                 >  SRR4302533.943005/1‑94 (MQ=60)
                 TTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCG                                                                              <  SRR4302533.4488154/94‑1 (MQ=60)
                  TTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGA                                                                             <  SRR4302533.3127324/94‑1 (MQ=60)
                   TGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAG                                                                            <  SRR4302533.534563/94‑1 (MQ=60)
                   TGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAG                                                                            <  SRR4302533.930777/94‑1 (MQ=60)
                    GCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGA                                                                           <  SRR4302533.5104975/94‑1 (MQ=60)
                     CCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGAT                                                                          >  SRR4302533.1265488/1‑94 (MQ=60)
                     CCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGAT                                                                          <  SRR4302533.2581359/94‑1 (MQ=60)
                      CGCGCTTTGCGCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATG                                                                         <  SRR4302533.658600/94‑1 (MQ=60)
                      CGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATG                                                                         >  SRR4302533.3038228/1‑94 (MQ=60)
                      CGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATG                                                                         >  SRR4302533.5331432/1‑94 (MQ=60)
                      CGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATG                                                                         >  SRR4302533.558061/1‑94 (MQ=60)
                          CTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACAC                                                                     <  SRR4302533.982275/94‑1 (MQ=60)
                          CTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACAC                                                                     <  SRR4302533.1214755/94‑1 (MQ=60)
                           TTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACA                                                                    <  SRR4302533.6029691/94‑1 (MQ=60)
                             TGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAG                                                                  >  SRR4302533.3303050/1‑94 (MQ=60)
                             TGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAG                                                                  <  SRR4302533.6030905/94‑1 (MQ=60)
                             TGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAG                                                                  <  SRR4302533.780681/94‑1 (MQ=60)
                               CCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAA                                                                <  SRR4302533.292353/94‑1 (MQ=60)
                                cggctgcgggtgtCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAAT                                                               <  SRR4302533.1792302/81‑1 (MQ=60)
                                CCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAAT                                                               <  SRR4302533.4106792/94‑1 (MQ=60)
                                CCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAAT                                                               <  SRR4302533.1792301/94‑1 (MQ=60)
                                CCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAAT                                                               >  SRR4302533.4585771/1‑94 (MQ=60)
                                 CGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATC                                                              <  SRR4302533.2051447/94‑1 (MQ=60)
                                  GCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCA                                                             <  SRR4302533.2186663/94‑1 (MQ=60)
                                   CTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCAC                                                            >  SRR4302533.505296/1‑94 (MQ=60)
                                   CTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCAC                                                            >  SRR4302533.5253439/1‑94 (MQ=60)
                                   CTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCAC                                                            >  SRR4302533.6022579/1‑94 (MQ=60)
                                    TGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACT                                                           <  SRR4302533.2646660/94‑1 (MQ=60)
                                    TGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACT                                                           >  SRR4302533.964715/1‑94 (MQ=60)
                                     GGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTT                                                          <  SRR4302533.152385/94‑1 (MQ=60)
                                      GGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTC                                                         <  SRR4302533.2689722/94‑1 (MQ=60)
                                       GGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCA                                                        <  SRR4302533.6233268/94‑1 (MQ=60)
                                       GGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCA                                                        <  SRR4302533.2484183/94‑1 (MQ=60)
                                        GCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAG                                                       <  SRR4302533.3894748/94‑1 (MQ=60)
                                        GCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAG                                                       >  SRR4302533.5853130/1‑94 (MQ=60)
                                         CTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGC                                                      <  SRR4302533.5900857/94‑1 (MQ=60)
                                         CTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGC                                                      >  SRR4302533.4875341/1‑94 (MQ=60)
                                            GCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAA                                                   <  SRR4302533.1010888/94‑1 (MQ=60)
                                            GCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAA                                                   <  SRR4302533.2468332/94‑1 (MQ=60)
                                              GTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGC                                                 <  SRR4302533.2886997/94‑1 (MQ=60)
                                               TTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCC                                                >  SRR4302533.1152628/1‑94 (MQ=60)
                                                TGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCG                                               <  SRR4302533.6292537/94‑1 (MQ=60)
                                                 GCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGC                                              >  SRR4302533.4329856/1‑94 (MQ=60)
                                                  CTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCT                                             >  SRR4302533.963281/1‑94 (MQ=60)
                                                   TGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTA                                            >  SRR4302533.1005810/1‑94 (MQ=60)
                                                   TGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTA                                            >  SRR4302533.4047200/1‑94 (MQ=60)
                                                   TGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTA                                            <  SRR4302533.5211306/94‑1 (MQ=60)
                                                    GGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTAT                                           >  SRR4302533.4630255/1‑94 (MQ=60)
                                                    GGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTAT                                           >  SRR4302533.4607713/1‑94 (MQ=60)
                                                     GTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATA                                          >  SRR4302533.3234503/1‑94 (MQ=60)
                                                     GTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATA                                          >  SRR4302533.4494333/1‑94 (MQ=60)
                                                       AGGATTTTCAATTCATGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGACGCTATATT                                        >  SRR4302533.1777981/1‑94 (MQ=60)
                                                           TTTTCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTA                                    >  SRR4302533.3177453/1‑94 (MQ=60)
                                                              TCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTG                                 >  SRR4302533.1468332/1‑94 (MQ=60)
                                                              TCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTG                                 <  SRR4302533.1541624/94‑1 (MQ=60)
                                                              TCAATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTG                                 <  SRR4302533.200462/94‑1 (MQ=60)
                                                                AATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGAT                               <  SRR4302533.1929253/94‑1 (MQ=60)
                                                                AATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGAT                               >  SRR4302533.2061729/1‑94 (MQ=60)
                                                                 ATTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATA                              <  SRR4302533.4794497/94‑1 (MQ=60)
                                                                  TTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAG                             <  SRR4302533.4751851/94‑1 (MQ=60)
                                                                  TTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAG                             <  SRR4302533.5747104/94‑1 (MQ=60)
                                                                  TTCCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAG                             >  SRR4302533.2167885/1‑94 (MQ=60)
                                                                    CCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGAGACAAGAATCACTTCAGCCAAGCCGCTATATTTTTACTGATAGCG                           >  SRR4302533.1137213/1‑94 (MQ=60)
                                                                    CCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCG                           >  SRR4302533.3068246/1‑94 (MQ=60)
                                                                    CCTGACAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCG                           <  SRR4302533.3223158/94‑1 (MQ=60)
                                                                     CTGACAGAGTTATGGAAGAAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGT                          <  SRR4302533.2886488/94‑1 (MQ=60)
                                                                       GACAGAGTGATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCG                        <  SRR4302533.3210863/94‑1 (MQ=60)
                                                                         CAGAGTTATGGAAGTAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGT                      <  SRR4302533.2018030/94‑1 (MQ=60)
                                                                         CAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGT                      <  SRR4302533.2661168/94‑1 (MQ=60)
                                                                         CAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGT                      >  SRR4302533.1509365/1‑94 (MQ=60)
                                                                         CAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGT                      >  SRR4302533.4453514/1‑94 (MQ=60)
                                                                         CAGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGT                      >  SRR4302533.3238931/1‑94 (MQ=60)
                                                                          AGAGTTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTG                     >  SRR4302533.2300725/1‑94 (MQ=60)
                                                                              TTATGGGAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGC                 >  SRR4302533.2303905/1‑94 (MQ=60)
                                                                              TTATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGC                 >  SRR4302533.501679/1‑94 (MQ=60)
                                                                               TATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCC                <  SRR4302533.1860397/94‑1 (MQ=60)
                                                                               TATGGAAGAAAACCGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCC                <  SRR4302533.3404186/94‑1 (MQ=60)
                                                                                ATGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCG               >  SRR4302533.702031/1‑94 (MQ=60)
                                                                                 TGGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGC              >  SRR4302533.2818433/1‑94 (MQ=60)
                                                                                  GGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCA             >  SRR4302533.57870/1‑94 (MQ=60)
                                                                                  GGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCA             >  SRR4302533.1066953/1‑94 (MQ=60)
                                                                                  GGAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCA             <  SRR4302533.4816258/94‑1 (MQ=60)
                                                                                   GAAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAG            >  SRR4302533.4046770/1‑94 (MQ=60)
                                                                                    AAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGG           >  SRR4302533.5106554/1‑94 (MQ=60)
                                                                                    AAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGG           <  SRR4302533.4877732/94‑1 (MQ=60)
                                                                                    AAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGG           <  SRR4302533.780968/94‑1 (MQ=60)
                                                                                    AAGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGCTGCCGCAGG           >  SRR4302533.2616735/1‑94 (MQ=60)
                                                                                     AGGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGT          <  SRR4302533.4121952/94‑1 (MQ=60)
                                                                                      GGAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTC         <  SRR4302533.1969607/94‑1 (MQ=60)
                                                                                       GAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCT        <  SRR4302533.1186730/94‑1 (MQ=60)
                                                                                       GAAAACGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCT        <  SRR4302533.5429598/94‑1 (MQ=60)
                                                                                            CGTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCAT   >  SRR4302533.4240869/1‑94 (MQ=60)
                                                                                             GTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATG  >  SRR4302533.3605696/1‑94 (MQ=60)
                                                                                             GTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATG  >  SRR4302533.3495217/1‑94 (MQ=60)
                                                                                             GTCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATG  >  SRR4302533.5771399/1‑94 (MQ=60)
                                                                                             ATCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATG  >  SRR4302533.1531321/1‑94 (MQ=60)
                                                                                             |                                                                                             
TCGAAAAGACCTGGCCTTTTGCCGCGCTTTGCCCGCTGGGGCTGGCGTTGCTGGTAGGATTTTCAATTCCTGACAGAGTTATGGAAGGAAAACATCCGCAATTTTTTGTCGAGATGACACAAGAATCACTTCAGCCAAGCCGCTATATTCTTACTGATAGCGTCGGTGTTGCCGCAGGTCTGGCATG  >  NC_000913/2370135‑2370321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑