Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,376,790	+C	coding (490/1671 nt)	menD ←	2‑oxoglutarate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,376,789	1	.	C	80.6%	408.7 / 102.4	160	coding (491/1671 nt)	menD	2‑oxoglutarate decarboxylase
Reads supporting (aligned to +/- strand): ref base . (16/15); new base C (61/68); total (77/83)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

Only 100 of 187 total aligned reads displayed.

CCTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑CT‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGA  >  NC_000913/2376697‑2376877

ccTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑a                                                                                                >  1:1715133/1‑93 (MQ=255)

 cTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑c                                                                                              <  2:4910716/93‑1 (MQ=255)

 cTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑c                                                                                              >  1:3026020/1‑93 (MQ=255)

  tGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑cc                                                                                             <  1:450361/93‑2 (MQ=255)

   gCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑ccc                                                                                            <  1:196052/93‑3 (MQ=255)

     caccaGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑Cc‑cc‑c                                                                                        <  1:5024812/93‑1 (MQ=255)

      accaGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑cc‑cc‑cc                                                                                       <  2:3408281/93‑1 (MQ=255)

      accaGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑cc‑cc‑cc                                                                                       <  1:2978687/93‑1 (MQ=255)

       ccaGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑Cc‑cc‑ccc                                                                                      <  1:3431948/93‑1 (MQ=255)

       ccaGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑Cc‑cc‑ccc                                                                                      >  2:2444971/1‑93 (MQ=255)

        caGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑cc‑cc‑cccc                                                                                     >  1:3311954/1‑93 (MQ=255)

            cACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑cc‑cc‑cccccgca                                                                                 <  1:4131281/93‑5 (MQ=255)

            cACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑cc‑cc‑cccccgca                                                                                 <  1:736830/93‑5 (MQ=255)

              cccAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATg                                                                              >  1:252646/1‑93 (MQ=255)

                cAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGaa                                                                            <  1:4287464/93‑1 (MQ=255)

                  gACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGc                                                                          >  1:3410622/1‑93 (MQ=255)

                  gACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGc                                                                          <  2:4855531/93‑1 (MQ=255)

                  gACGCTGTTGCAGCCAAAGCCGGGAATGTTCAATTTGCCCATACACGGGTTAACAAAACGGGCAGTGGATAT‑GA‑ACCC‑CC‑CCCC‑GC‑ATGAAGc                                                                          >  2:2900582/1‑93 (MQ=255)

                   aCGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GT‑ACCC‑CC‑CCCC‑GC‑ATGAAGCg                                                                         <  2:3588290/93‑1 (MQ=255)

                   aCGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCg                                                                         <  1:5789825/93‑1 (MQ=255)

                   aCGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑CC‑CC‑CCCCACC‑ATGAAGCg                                                                         >  1:5112839/1‑93 (MQ=255)

                   aCGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑CC‑CC‑CCCC‑GC‑ATGAAGCGt                                                                        <  1:1260951/93‑1 (MQ=255)

                   aCGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑CC‑CC‑CCCC‑CC‑ATGAAGCGt                                                                        >  2:4052383/1‑93 (MQ=255)

                     gCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTa                                                                       >  1:5350526/1‑93 (MQ=255)

                     gCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTa                                                                       >  1:2331576/1‑93 (MQ=255)

                     gCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTa                                                                       <  1:133792/93‑1 (MQ=255)

                     gCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTa                                                                       >  1:265277/1‑93 (MQ=255)

                      cTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTAc                                                                      >  2:4528401/1‑93 (MQ=255)

                          tGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCgag                                                                  >  2:6255300/1‑93 (MQ=255)

                           gCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTCCCgaga                                                                 <  1:3499468/93‑1 (MQ=255)

                           gCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCgaga                                                                 >  2:706625/1‑93 (MQ=255)

                           gCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑CC‑CC‑CCCC‑GC‑ATGAAGCGTACCGagag                                                                >  1:3705848/1‑93 (MQ=255)

                            ccAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGagag                                                                <  2:4385745/93‑1 (MQ=255)

                             cAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGCTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGc                                                               >  1:2788548/1‑93 (MQ=255)

                               gCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGt                                                             <  2:2287338/93‑1 (MQ=255)

                               gCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑CC‑CCACCCC‑GC‑ATGAAGCGTACCGAGAGCGt                                                             >  2:5858970/1‑93 (MQ=255)

                               gCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑CC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTg                                                            <  2:4934637/93‑1 (MQ=255)

                                cTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTg                                                            <  1:4786582/93‑1 (MQ=255)

                                 tAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GGCC‑CC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTgg                                                           <  2:2648911/93‑1 (MQ=255)

                                 tAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTgg                                                           >  1:1128615/1‑93 (MQ=255)

                                    gCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCg                                                        >  2:2938648/1‑93 (MQ=255)

                                     cccGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGa                                                       <  2:5396135/93‑1 (MQ=255)

                                      ccGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GGCC‑CC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGAt                                                      <  1:574573/93‑1 (MQ=255)

                                       cGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATg                                                     <  1:4704883/93‑1 (MQ=255)

                                       cGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATg                                                     >  2:3990702/1‑93 (MQ=255)

                                       cGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAAAGCGTGGACAATg                                                     >  2:1753897/1‑93 (MQ=255)

                                        ggTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATgg                                                    <  1:5001780/93‑1 (MQ=255)

                                         gTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGt                                                   >  2:2388747/1‑93 (MQ=255)

                                          tATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTa                                                  >  2:2443771/1‑93 (MQ=255)

                                           aTCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAg                                                 >  1:1688017/1‑93 (MQ=255)

                                            tCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGa                                                >  1:5088084/1‑93 (MQ=255)

                                             cGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGa‑a                                              <  1:3711042/93‑1 (MQ=255)

                                             cGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGa‑a                                              >  1:3570314/1‑93 (MQ=255)

                                              gTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGa‑aa                                             >  2:3150526/1‑93 (MQ=255)

                                               tCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTGGA‑AAc                                            >  2:5957784/1‑93 (MQ=255)

                                               tCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AAc                                            <  2:4588100/93‑1 (MQ=255)

                                                 cATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCa                                          >  2:1546223/1‑93 (MQ=255)

                                                 cATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCa                                          <  1:2286146/93‑1 (MQ=255)

                                                 cATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCa                                          <  1:3285029/93‑1 (MQ=255)

                                                      cGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCaa                                     >  2:1748062/1‑93 (MQ=255)

                                                      cGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑CC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAAc                                    <  2:400031/93‑1 (MQ=255)

                                                       gCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAAc                                    >  1:6247447/1‑93 (MQ=255)

                                                       gCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAAc                                    <  1:3143631/93‑1 (MQ=255)

                                                        ccATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACg                                   <  1:5791907/93‑1 (MQ=255)

                                                        ccATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACg                                   >  2:3705002/1‑93 (MQ=255)

                                                         cATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGt                                  <  2:3144748/93‑1 (MQ=255)

                                                         cATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGt                                  <  2:5227667/93‑1 (MQ=255)

                                                          aTACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTAACGAGAGAGCGGTCGATGGTAGA‑AACCAGCCAACGTg                                 >  2:1433016/1‑93 (MQ=255)

                                                           tACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GGCC‑CC‑CC‑CCCC‑GC‑ATGAAGCGTCCCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGc                                <  1:4240137/93‑1 (MQ=255)

                                                           tACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGc                                >  2:2512328/1‑93 (MQ=255)

                                                           tACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGc                                >  2:678081/1‑93 (MQ=255)

                                                            aCAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCg                               >  1:160511/1‑93 (MQ=255)

                                                              aGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGGGTACCGAAAGCGTGGTCGATGGCAGA‑AACCACGCAACGCGCggg                             >  2:5589220/1‑93 (MQ=255)

                                                              aGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCggg                             <  1:5275956/93‑1 (MQ=255)

                                                               gCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGCGGACCCGGTCGATGGTAGA‑AACGCGCCAACGTGCgggg                            >  2:1665733/1‑93 (MQ=255)

                                                               gCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCgggg                            <  1:1938494/93‑1 (MQ=255)

                                                                cGGTTCAGCAAACGGGCAGTTGATAG‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGa                           <  1:2595063/93‑1 (MQ=255)

                                                                  ggtCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAACGTGGAGCAT‑GCAGAGAACCCGCCAACGTGCGGGGtta                         >  2:4271138/3‑93 (MQ=255)

                                                                  gTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGata                         <  2:4688121/93‑1 (MQ=255)

                                                                  gTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGata                         <  1:974382/93‑1 (MQ=255)

                                                                  gTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑CCCCC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGatt                         >  1:211229/1‑92 (MQ=255)

                                                                    tCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATc                       <  2:38116/93‑1 (MQ=255)

                                                                    tCAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATc                       >  2:149572/1‑93 (MQ=255)

                                                                     cAGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATcc                      >  2:2694435/1‑93 (MQ=255)

                                                                      aGCAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATCCt                     >  1:6282890/1‑93 (MQ=255)

                                                                        cAAACGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATCCTgg                   <  2:38881/93‑1 (MQ=255)

                                                                         aaaCGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATCCTggg                  <  2:832726/93‑1 (MQ=255)

                                                                          aaCGGGCAGTTGATAT‑GG‑A‑CC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATCCTGGGTc                >  2:1954872/1‑93 (MQ=255)

                                                                            cGGGCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATCCTGGGTCg               <  1:5562282/93‑1 (MQ=255)

                                                                              ggCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATCCTGGGTCggg             <  2:4862621/93‑1 (MQ=255)

                                                                              ggCAGTTGATAT‑GG‑A‑CC‑CC‑CCCC‑CCAATGAAGCGTACCGAGAGCGGGGTCGATGGGAGA‑AACCAGCCAACGTGCGGGGAGACCCTGGGTCggg             >  2:4881050/1‑93 (MQ=255)

                                                                               gCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATCCTGGGTCGGgc            <  1:3750372/93‑1 (MQ=255)

                                                                               gCAGTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATCCTGGGTCGGgc            <  2:6173573/93‑1 (MQ=255)

                                                                                 aGTTGATATGGG‑C‑CC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATCCTGGGTCGGgcgc          <  1:4192494/93‑1 (MQ=255)

                                                                                  gTTGATAT‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATCCTGGGTCGGGcgcg         >  1:4008630/1‑93 (MQ=255)

                                                                                      atat‑GG‑ACCC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCaa     <  2:278273/93‑1 (MQ=255)

                                                                                      atat‑GG‑A‑CCCCC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCaa     >  2:4986960/1‑93 (MQ=255)

                                                                                       tat‑GG‑A‑CCCCC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAAt    >  1:1412451/1‑93 (MQ=255)

                                                                                       tat‑GG‑A‑CC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATg   <  1:3559707/93‑1 (MQ=255)

                                                                                        at‑GG‑A‑CC‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGa  <  1:5624850/93‑1 (MQ=255)

CCTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑CT‑CC‑CCCC‑GC‑ATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA‑AACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGA  >  NC_000913/2376697‑2376877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CTC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACT  >  NC_000913/2376697‑2376883

CCTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑C                                                                                                  >  SRR4302533.3044538/1‑94 (MQ=60)

 CTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CC                                                                                                 >  SRR4302533.5621819/1‑94 (MQ=60)

 CTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGCATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CC                                                                                                 >  SRR4302533.1753305/1‑94 (MQ=60)

  TGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCC                                                                                                <  SRR4302533.453055/94‑1 (MQ=60)

   GCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCC‑C                                                                                              <  SRR4302533.197290/94‑1 (MQ=60)

    CCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCC‑CC                                                                                             >  SRR4302533.4270631/1‑94 (MQ=60)

     CACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCC‑CC‑C                                                                                           >  SRR4302533.5006527/1‑94 (MQ=60)

     CACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCC‑CC‑C                                                                                           <  SRR4302533.5055239/94‑1 (MQ=60)

     CACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCC‑CC‑C                                                                                           >  SRR4302533.2405262/1‑94 (MQ=60)

     CACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCC‑CC‑C                                                                                           >  SRR4302533.1993484/1‑94 (MQ=60)

      ACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCC‑CC‑CC                                                                                          <  SRR4302533.2996911/94‑1 (MQ=60)

       GCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCC‑CC‑CCC                                                                                         >  SRR4302533.5523401/1‑94 (MQ=60)

       CCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCC‑CC‑CCC                                                                                         <  SRR4302533.3453150/94‑1 (MQ=60)

       CCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCC‑CC‑CCC                                                                                         >  SRR4302533.3332407/1‑94 (MQ=60)

            CACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCAT                                                                                    <  SRR4302533.741164/94‑1 (MQ=60)

            CACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCAT                                                                                    <  SRR4302533.4156356/94‑1 (MQ=60)

             ACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATG                                                                                   >  SRR4302533.254199/1‑94 (MQ=60)

               CCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑ACCCCC‑CC‑CCC‑GCATGA                                                                                  >  SRR4302533.5811435/1‑94 (MQ=60)

               CCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAA                                                                                 >  SRR4302533.5133308/1‑94 (MQ=60)

                CAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAG                                                                                <  SRR4302533.4313245/94‑1 (MQ=60)

                 AGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGC                                                                               >  SRR4302533.34357/1‑94 (MQ=60)

                 AGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGC                                                                               >  SRR4302533.3431686/1‑94 (MQ=60)

                 AGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGC                                                                               <  SRR4302533.4725991/94‑1 (MQ=60)

                  GACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCC‑CC‑CCCACCATGAAGCG                                                                              >  SRR4302533.5143736/1‑94 (MQ=60)

                   GCGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGT                                                                             <  SRR4302533.3211178/94‑1 (MQ=60)

                   ACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATGCAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCC‑CCGCCC‑GCCTGTAGCGT                                                                             >  SRR4302533.741682/1‑94 (MQ=60)

                   ACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGT                                                                             <  SRR4302533.5824806/94‑1 (MQ=60)

                   ACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCC‑CC‑CCC‑GCATGAAGCGTA                                                                            <  SRR4302533.1268492/94‑1 (MQ=60)

                    CGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTA                                                                            >  SRR4302533.2346010/1‑94 (MQ=60)

                    CGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTA                                                                            >  SRR4302533.5382632/1‑94 (MQ=60)

                    CGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTA                                                                            >  SRR4302533.266890/1‑94 (MQ=60)

                    CGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTA                                                                            <  SRR4302533.679617/94‑1 (MQ=60)

                     GCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTAC                                                                           <  SRR4302533.134637/94‑1 (MQ=60)

                      CTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCC‑CC‑CCC‑GCATGAAGCGTACCG                                                                         <  SRR4302533.4033282/94‑1 (MQ=60)

                          TGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAG                                                                     >  SRR4302533.3728612/1‑94 (MQ=60)

                           GCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTCCCGAGAG                                                                     <  SRR4302533.3521049/94‑1 (MQ=60)

                           GCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAG                                                                     <  SRR4302533.5997449/94‑1 (MQ=60)

                            CCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGCTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGC                                                                    >  SRR4302533.2805688/1‑94 (MQ=60)

                               GCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTG                                                                 <  SRR4302533.6298069/94‑1 (MQ=60)

                                CTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGG                                                                <  SRR4302533.4815570/94‑1 (MQ=60)

                                CTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGG                                                                >  SRR4302533.3110816/1‑94 (MQ=60)

                                CTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGG                                                                >  SRR4302533.1135321/1‑94 (MQ=60)

                                 TAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTC                                                              >  SRR4302533.3310876/1‑94 (MQ=60)

                                  AAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTC                                                              <  SRR4302533.2585906/94‑1 (MQ=60)

                                      CCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GGCC‑‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATG                                                          <  SRR4302533.577965/94‑1 (MQ=60)

                                       CGTTAACGTCCATTTCGCTATACAGCGTTTCAGCAACGGGGCAGTTGATAT‑GG‑A‑‑CCC‑CC‑CCC‑GCATGAAGCGGACCGAGAGCGTGGTCGATGGT                                                        <  SRR4302533.4427889/94‑1 (MQ=60)

                                       CGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGG                                                         <  SRR4302533.4733400/94‑1 (MQ=60)

                                        GGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGT                                                        <  SRR4302533.5032100/94‑1 (MQ=60)

                                        GGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGT                                                        >  SRR4302533.4803484/1‑94 (MQ=60)

                                          TATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAG                                                      >  SRR4302533.226555/1‑94 (MQ=60)

                                          TATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAG                                                      >  SRR4302533.1698246/1‑94 (MQ=60)

                                          TATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAG                                                      <  SRR4302533.3964061/94‑1 (MQ=60)

                                           ATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGA                                                     >  SRR4302533.5118828/1‑94 (MQ=60)

                                            TCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAA                                                    >  SRR4302533.3592318/1‑94 (MQ=60)

                                             CGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAGA                                                   >  SRR4302533.3037635/1‑94 (MQ=60)

                                             CGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAA                                                   <  SRR4302533.3733832/94‑1 (MQ=60)

                                              GTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAAC                                                  >  SRR4302533.2646728/1‑94 (MQ=60)

                                              GTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAAC                                                  >  SRR4302533.3732096/1‑94 (MQ=60)

                                                CCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCA                                                >  SRR4302533.279971/1‑94 (MQ=60)

                                                CCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCC‑CC‑CCCccccataaggggacccggagcgggggcggtggtaggaaaca                                                >  SRR4302533.2248154/1‑53 (MQ=60)

                                                CCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAG                                               >  SRR4302533.1709446/1‑94 (MQ=60)

                                                 CATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAG                                               <  SRR4302533.3305305/94‑1 (MQ=60)

                                                 CATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAG                                               <  SRR4302533.2300295/94‑1 (MQ=60)

                                                 CATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CCCTCC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAG                                               >  SRR4302533.3713372/1‑94 (MQ=60)

                                                   TTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCC                                             <  SRR4302533.161667/94‑1 (MQ=60)

                                                      CGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAAC                                          >  SRR4302533.6284833/1‑94 (MQ=60)

                                                       GCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACG                                         <  SRR4302533.3162955/94‑1 (MQ=60)

                                                        CCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGGTGGTAGAAACCAGCCCACGT                                        >  SRR4302533.3414139/1‑94 (MQ=60)

                                                        CCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGT                                        <  SRR4302533.5826898/94‑1 (MQ=60)

                                                           TACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GGCC‑‑CCC‑CC‑CCC‑GCATGAAGCGTCCCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCG                                     <  SRR4302533.4265745/94‑1 (MQ=60)

                                                           TACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCG                                     >  SRR4302533.161537/1‑94 (MQ=60)

                                                           TACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCG                                     >  SRR4302533.1496413/1‑94 (MQ=60)

                                                            ACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGG                                    <  SRR4302533.2324828/94‑1 (MQ=60)

                                                              AGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGG                                  <  SRR4302533.5307588/94‑1 (MQ=60)

                                                               GCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGA                                 <  SRR4302533.1270717/94‑1 (MQ=60)

                                                               GCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGA                                 <  SRR4302533.1950312/94‑1 (MQ=60)

                                                                CGGTTCAGCAAACGGGCAGTTGATAG‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGAT                                <  SRR4302533.2611012/94‑1 (MQ=60)

                                                                 GGTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATT                               >  SRR4302533.212561/1‑94 (MQ=60)

                                                                  GTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATAT                              <  SRR4302533.5785460/94‑1 (MQ=60)

                                                                  GTTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATAT                              <  SRR4302533.980102/94‑1 (MQ=60)

                                                                   TTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGCGAGGGTGGACGATGGAAGAAACGAGCCAACGTGCGGGGATATC                             >  SRR4302533.4226962/1‑94 (MQ=60)

                                                                   TTCAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATC                             <  SRR4302533.5035900/94‑1 (MQ=60)

                                                                     CAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCT                           >  SRR4302533.5676967/1‑94 (MQ=60)

                                                                     CAGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCT                           >  SRR4302533.6320384/1‑94 (MQ=60)

                                                                      AGCAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTG                          >  SRR4302533.3587520/1‑94 (MQ=60)

                                                                       GCAAAGGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGG                         <  SRR4302533.3771696/94‑1 (MQ=60)

                                                                        CAAACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGGGGACCGAGAGCGTGGTCGATGGCAGGAACCAGCCAgcgtgaggcgatatgatggt                        >  SRR4302533.4062904/1‑74 (MQ=44)

                                                                          AACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTC                      <  SRR4302533.3499548/94‑1 (MQ=60)

                                                                          AACGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTC                      <  SRR4302533.1370711/94‑1 (MQ=60)

                                                                            CGGGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGG                    <  SRR4302533.5595901/94‑1 (MQ=60)

                                                                              GGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGC                  <  SRR4302533.53799/94‑1 (MQ=60)

                                                                              GGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGC                  <  SRR4302533.4377413/94‑1 (MQ=60)

                                                                              GGCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGC                  <  SRR4302533.5207075/94‑1 (MQ=60)

                                                                               GCAGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCG                 <  SRR4302533.3773366/94‑1 (MQ=60)

                                                                               GCAGTAGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCG                 <  SRR4302533.895922/94‑1 (MQ=60)

                                                                                 AGTTGATATGGG‑C‑‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCG               <  SRR4302533.4217870/94‑1 (MQ=60)

                                                                                 AGTTGATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCG               >  SRR4302533.4033038/1‑94 (MQ=60)

                                                                                     GATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGACGGTAGAAACCAGCCAACGTACGGGGATATCCTGGGTCGGGCGCGGCAA           >  SRR4302533.4557675/1‑94 (MQ=60)

                                                                                      ATAT‑GG‑AC‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAAT          >  SRR4302533.1420992/1‑94 (MQ=60)

                                                                                       TAT‑GG‑A‑‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGA        <  SRR4302533.3581640/94‑1 (MQ=60)

                                                                                        AT‑GG‑A‑‑CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAA       <  SRR4302533.5658847/94‑1 (MQ=60)

                                                                                                 CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACT  <  SRR4302533.1653651/94‑1 (MQ=60)

                                                                                                 CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACT  <  SRR4302533.6014448/94‑1 (MQ=60)

                                                                                                 CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACT  >  SRR4302533.3575049/1‑94 (MQ=60)

                                                                                                 CCC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACT  <  SRR4302533.2889203/94‑1 (MQ=60)

                                                                                                 CCC‑CC‑CCC‑GCATGAAGCGTAACGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACT  >  SRR4302533.4437228/1‑94 (MQ=60)

CCTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATAT‑GG‑A‑‑CTC‑CC‑CCC‑GCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACT  >  NC_000913/2376697‑2376883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`