Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,461,706 T→C S225S (AGT→AGC)  b2345 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,461,7060TC100.0% 855.9 / NA 261S225S (AGT→AGC) b2345hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (142/119);  total (142/119)

Only 100 of 263 total aligned reads displayed.

TTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGT  >  NC_000913/2461616‑2461795
                                                                                          |                                                                                         
ttACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGa                                                                                         >  2:2576688/1‑93 (MQ=255)
  aCTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaa                                                                                       >  2:6184390/1‑93 (MQ=255)
  aCTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaa                                                                                       >  2:5738396/1‑93 (MQ=255)
  aCTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaa                                                                                       <  2:2403520/93‑1 (MQ=255)
   cTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaaa                                                                                      <  1:5276085/93‑1 (MQ=255)
   cTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaaa                                                                                      >  1:3839005/1‑93 (MQ=255)
   cTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaaa                                                                                      <  2:1515404/93‑1 (MQ=255)
   cTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaaa                                                                                      <  2:1929442/93‑1 (MQ=255)
    ttATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAAc                                                                                     <  2:4286635/93‑1 (MQ=255)
    ttATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAAc                                                                                     >  1:2818968/1‑93 (MQ=255)
    ttATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAAc                                                                                     >  1:5831476/1‑93 (MQ=255)
     tATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACg                                                                                    <  1:6208093/93‑1 (MQ=255)
     tATCTCTCGGCTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACg                                                                                    <  1:3233602/93‑1 (MQ=255)
       tctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGcc                                                                                  >  2:5080813/1‑93 (MQ=255)
       tctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGcc                                                                                  <  2:1385144/93‑1 (MQ=255)
       tctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGcc                                                                                  >  1:4421398/1‑93 (MQ=255)
        ctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCa                                                                                 <  1:2491525/93‑1 (MQ=255)
         tctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAg                                                                                >  1:2520350/1‑93 (MQ=255)
         tctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAg                                                                                <  1:828167/93‑1 (MQ=255)
         tctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAg                                                                                <  2:1609767/93‑1 (MQ=255)
         tctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAg                                                                                >  1:1731254/1‑93 (MQ=255)
         tctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAg                                                                                >  2:514690/1‑93 (MQ=255)
          ctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAgg                                                                               >  1:2936608/1‑93 (MQ=255)
           tcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACTCAATATTGTAGCGCAAACGCCAGGa                                                                              >  2:862174/1‑93 (MQ=255)
           tcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGa                                                                              <  2:671303/93‑1 (MQ=255)
           tcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGa                                                                              <  1:4867094/93‑1 (MQ=255)
            cGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAg                                                                             <  1:5700936/93‑1 (MQ=255)
             ggTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGt                                                                            >  1:739736/1‑93 (MQ=255)
             ggTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGt                                                                            >  2:5601375/1‑93 (MQ=255)
             ggTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGt                                                                            >  1:5684826/1‑93 (MQ=255)
             ggTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGt                                                                            <  1:5439569/93‑1 (MQ=255)
                  ctacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTc                                                                       <  2:282271/93‑1 (MQ=255)
                   tacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCa                                                                      >  1:5173751/1‑93 (MQ=255)
                   tacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCa                                                                      <  1:1411462/93‑1 (MQ=255)
                       aTCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGt                                                                  >  1:4381951/1‑93 (MQ=255)
                       aTCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGt                                                                  <  1:1857684/93‑1 (MQ=255)
                        tCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTg                                                                 >  1:423012/1‑93 (MQ=255)
                        tCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTg                                                                 >  2:5850806/1‑93 (MQ=255)
                          aGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGt                                                               <  2:745440/93‑1 (MQ=255)
                           ggACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGtt                                                              <  2:551288/93‑1 (MQ=255)
                              cgacTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGa                                                           >  1:4804011/1‑93 (MQ=255)
                               gacTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGaa                                                          <  2:4239008/93‑1 (MQ=255)
                               gacTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGaa                                                          >  2:5219954/1‑93 (MQ=255)
                                acTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGaaa                                                         <  1:2033839/93‑1 (MQ=255)
                                  tACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGc                                                       <  1:1896905/93‑1 (MQ=255)
                                    ccACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAg                                                     <  1:3992335/93‑1 (MQ=255)
                                      acaTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTa                                                   <  1:682404/93‑1 (MQ=255)
                                        aTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTaaa                                                 >  2:3245985/1‑93 (MQ=255)
                                         ttCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAg                                                <  1:1259876/93‑1 (MQ=255)
                                          tCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGc                                               <  2:605356/93‑1 (MQ=255)
                                            aaaGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTa                                             >  2:2727817/1‑93 (MQ=255)
                                            aaaGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTa                                             >  1:4727787/1‑93 (MQ=255)
                                             aaGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTAt                                            >  1:5345130/1‑93 (MQ=255)
                                             aaGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTAt                                            >  1:5181302/1‑93 (MQ=255)
                                             aaGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTAt                                            >  1:1021793/1‑93 (MQ=255)
                                             aaGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTAt                                            >  1:662155/1‑93 (MQ=255)
                                              agagGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATc                                           <  1:6215245/93‑1 (MQ=255)
                                               gagGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCa                                          >  2:4149324/1‑93 (MQ=255)
                                               gagGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCa                                          <  2:3862704/93‑1 (MQ=255)
                                               gagGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCa                                          >  1:5045106/1‑93 (MQ=255)
                                                agGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCAt                                         <  1:4144685/93‑1 (MQ=255)
                                                 ggAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATg                                        >  2:3392549/1‑93 (MQ=255)
                                                 ggAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATg                                        <  2:5777481/93‑1 (MQ=255)
                                                  gAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATgg                                       >  2:2600765/1‑93 (MQ=255)
                                                  gAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATgg                                       <  1:1108724/93‑1 (MQ=255)
                                                    aTTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCa                                     <  1:5592801/93‑1 (MQ=255)
                                                      tCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGa                                   <  1:6022444/93‑1 (MQ=255)
                                                      tCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGa                                   <  2:5400812/93‑1 (MQ=255)
                                                        gTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAAt                                 >  1:2187559/1‑93 (MQ=255)
                                                           ttAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAt                              >  2:2349391/1‑93 (MQ=255)
                                                             aaTGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAttt                            >  2:468298/1‑93 (MQ=255)
                                                              aTGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTg                           >  2:1095159/1‑93 (MQ=255)
                                                              aTGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTg                           >  1:5818907/1‑93 (MQ=255)
                                                                 gTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCaa                        <  2:2918152/93‑1 (MQ=255)
                                                                  tGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCaat                       >  1:3135347/1‑92 (MQ=255)
                                                                  tGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAAc                       <  2:4036191/93‑1 (MQ=255)
                                                                  tGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAAc                       <  1:2118694/93‑1 (MQ=255)
                                                                  tGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAAc                       >  1:354274/1‑93 (MQ=255)
                                                                   gATAGTCGCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACt                      <  2:4380699/93‑1 (MQ=255)
                                                                    aTAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTAGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTg                     <  2:495114/93‑1 (MQ=255)
                                                                     tAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTgg                    <  2:1829400/93‑1 (MQ=255)
                                                                      aGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGt                   <  2:497404/93‑1 (MQ=255)
                                                                      aGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGt                   <  1:2353169/93‑1 (MQ=255)
                                                                         ctcACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTcc                <  1:1543214/93‑1 (MQ=255)
                                                                         ctcACCCAATATTGTAGCGAAAACGCCAGGAGAGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTcc                >  2:2108548/1‑93 (MQ=255)
                                                                            aCCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGat             >  2:3476468/1‑93 (MQ=255)
                                                                             cccAATATTGTAGCGAAAACGCCAGGAGGGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatc            >  2:4018719/1‑93 (MQ=255)
                                                                              ccAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatca           >  2:4835231/1‑93 (MQ=255)
                                                                               cAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatcat          >  1:3403105/1‑93 (MQ=255)
                                                                                aaTATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatcatc         >  2:3196200/1‑93 (MQ=255)
                                                                                aaTATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatcatc         >  1:2686032/1‑93 (MQ=255)
                                                                                 atatTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGAtcatca        <  2:2998224/93‑1 (MQ=255)
                                                                                  tatTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAg       >  1:275782/1‑93 (MQ=255)
                                                                                  tatTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAg       <  2:207073/93‑1 (MQ=255)
                                                                                    ttGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGt     <  1:3060388/93‑1 (MQ=255)
                                                                                    ttGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGt     <  2:1055578/93‑1 (MQ=255)
                                                                                    ttGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGt     >  1:118089/1‑93 (MQ=255)
                                                                                      gTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTg   >  2:6123529/1‑93 (MQ=255)
                                                                                      gTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTg   >  2:2020780/1‑93 (MQ=255)
                                                                                       tAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGt  <  1:1569650/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGT  >  NC_000913/2461616‑2461795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTA  >  NC_000913/2461614‑2461796
                                                                                            |                                                                                          
GATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCG                                                                                           <  SRR4302533.1760890/94‑1 (MQ=60)
GATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCG                                                                                           >  SRR4302533.1772580/1‑94 (MQ=60)
GATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCG                                                                                           >  SRR4302533.5361598/1‑94 (MQ=60)
GATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCG                                                                                           >  SRR4302533.5211557/1‑94 (MQ=60)
 ATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGA                                                                                          >  SRR4302533.1358647/1‑94 (MQ=60)
 ATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGA                                                                                          >  SRR4302533.430880/1‑94 (MQ=60)
   TACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAA                                                                                        >  SRR4302533.1155047/1‑94 (MQ=60)
   TACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAA                                                                                        <  SRR4302533.15792/94‑1 (MQ=60)
    ACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAA                                                                                       <  SRR4302533.2524924/94‑1 (MQ=60)
    ACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAA                                                                                       >  SRR4302533.3862457/1‑94 (MQ=60)
     CTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAAC                                                                                      >  SRR4302533.5866721/1‑94 (MQ=60)
     CTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAAC                                                                                      >  SRR4302533.2836314/1‑94 (MQ=60)
     CTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAAC                                                                                      >  SRR4302533.4873884/1‑94 (MQ=60)
     CTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAAC                                                                                      >  SRR4302533.5917751/1‑94 (MQ=60)
     CTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAAC                                                                                      <  SRR4302533.5307717/94‑1 (MQ=60)
      TTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACG                                                                                     >  SRR4302533.369241/1‑94 (MQ=60)
       TATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGC                                                                                    >  SRR4302533.3438409/1‑94 (MQ=60)
       TATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGC                                                                                    <  SRR4302533.6245329/94‑1 (MQ=60)
       TATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGC                                                                                    >  SRR4302533.5697106/1‑94 (MQ=60)
       TATCTCTCGGCTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGC                                                                                    <  SRR4302533.3253527/94‑1 (MQ=60)
        ATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCC                                                                                   >  SRR4302533.4448128/1‑94 (MQ=60)
          CTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAG                                                                                 >  SRR4302533.1741750/1‑94 (MQ=60)
          CTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAG                                                                                 >  SRR4302533.3137958/1‑94 (MQ=60)
          CTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAG                                                                                 >  SRR4302533.649141/1‑94 (MQ=60)
          CTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAG                                                                                 >  SRR4302533.2870990/1‑94 (MQ=60)
          CTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAG                                                                                 >  SRR4302533.2776425/1‑94 (MQ=60)
          CTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAG                                                                                 >  SRR4302533.2776424/1‑94 (MQ=60)
          CTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAG                                                                                 >  SRR4302533.2535879/1‑94 (MQ=60)
          CTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAG                                                                                 <  SRR4302533.2506870/94‑1 (MQ=60)
          CTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAG                                                                                 <  SRR4302533.3900537/94‑1 (MQ=60)
          CTCTCGGTTACTACGATCAGGACAACTACCACATCAAAAGAGGAATTCGTATTAATGGTGATAGCCTCACCCAATATTGTAGCGAAAACGCCAG                                                                                 >  SRR4302533.1960487/1‑94 (MQ=60)
           TCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGG                                                                                <  SRR4302533.833024/94‑1 (MQ=60)
           TCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGG                                                                                >  SRR4302533.2954560/1‑94 (MQ=60)
             TCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAG                                                                              <  SRR4302533.2135195/94‑1 (MQ=60)
             TCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAG                                                                              <  SRR4302533.546666/94‑1 (MQ=60)
             TCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAG                                                                              <  SRR4302533.4896586/94‑1 (MQ=60)
              CGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGT                                                                             >  SRR4302533.5719162/1‑94 (MQ=60)
              CGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGT                                                                             >  SRR4302533.3030714/1‑94 (MQ=60)
              CGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGT                                                                             <  SRR4302533.5735362/94‑1 (MQ=60)
              CGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGT                                                                             <  SRR4302533.1893109/94‑1 (MQ=60)
              CGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGT                                                                             >  SRR4302533.744089/1‑94 (MQ=60)
               GGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTG                                                                            <  SRR4302533.5472328/94‑1 (MQ=60)
                GTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGC                                                                           >  SRR4302533.3050424/1‑94 (MQ=60)
                  TACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTT                                                                         >  SRR4302533.6133347/1‑94 (MQ=60)
                   ACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTC                                                                        >  SRR4302533.4132555/1‑94 (MQ=60)
                    CTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCA                                                                       >  SRR4302533.5205006/1‑94 (MQ=60)
                     TACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAG                                                                      <  SRR4302533.1419995/94‑1 (MQ=60)
                     TACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAG                                                                      >  SRR4302533.5230352/1‑94 (MQ=60)
                        GATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGT                                                                   >  SRR4302533.4408408/1‑94 (MQ=60)
                         ATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTG                                                                  >  SRR4302533.425543/1‑94 (MQ=60)
                         ATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTG                                                                  <  SRR4302533.1869014/94‑1 (MQ=60)
                          TCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGG                                                                 <  SRR4302533.4531234/94‑1 (MQ=60)
                             GGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTT                                                              >  SRR4302533.1987011/1‑94 (MQ=60)
                             GGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTT                                                              <  SRR4302533.2451118/94‑1 (MQ=60)
                             GGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGAGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGGGCTTCAGCGTGGTTT                                                              >  SRR4302533.2673658/1‑94 (MQ=60)
                              GACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTG                                                             <  SRR4302533.177757/94‑1 (MQ=60)
                              GACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTG                                                             >  SRR4302533.2578223/1‑94 (MQ=60)
                               ACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGA                                                            >  SRR4302533.4833103/1‑94 (MQ=60)
                                CGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAA                                                           >  SRR4302533.5820973/1‑94 (MQ=60)
                                CGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAA                                                           >  SRR4302533.414052/1‑94 (MQ=60)
                                CGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAA                                                           <  SRR4302533.5233451/94‑1 (MQ=60)
                                 GACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAA                                                          >  SRR4302533.1527959/1‑94 (MQ=60)
                                 GACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAA                                                          <  SRR4302533.5575668/94‑1 (MQ=60)
                                  ACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAG                                                         >  SRR4302533.1748145/1‑94 (MQ=60)
                                  ACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAG                                                         <  SRR4302533.1303491/94‑1 (MQ=60)
                                  ACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAG                                                         <  SRR4302533.800812/94‑1 (MQ=60)
                                  ACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAG                                                         <  SRR4302533.2046385/94‑1 (MQ=60)
                                   CTAGCACACTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCGCCCAATATTGTAGCGAAAACGCCAGGAGTGCGTCAGCGTGGTTTGAAAGC                                                        <  SRR4302533.6252370/94‑1 (MQ=60)
                                    TACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCA                                                       <  SRR4302533.1908485/94‑1 (MQ=60)
                                    TACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCA                                                       <  SRR4302533.734908/94‑1 (MQ=60)
                                     ACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAG                                                      <  SRR4302533.4763163/94‑1 (MQ=60)
                                     ACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAG                                                      >  SRR4302533.433820/1‑94 (MQ=60)
                                      CCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGT                                                     <  SRR4302533.4016645/94‑1 (MQ=60)
                                        ACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAA                                                   >  SRR4302533.5205397/1‑94 (MQ=60)
                                        ACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAA                                                   <  SRR4302533.696948/94‑1 (MQ=60)
                                        ACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAA                                                   <  SRR4302533.686440/94‑1 (MQ=60)
                                          ATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAG                                                 >  SRR4302533.2521113/1‑94 (MQ=60)
                                           TTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGC                                                <  SRR4302533.1267413/94‑1 (MQ=60)
                                             CAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTA                                              >  SRR4302533.386615/1‑94 (MQ=60)
                                             CAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTA                                              >  SRR4302533.4756448/1‑94 (MQ=60)
                                              AAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTAT                                             >  SRR4302533.666061/1‑94 (MQ=60)
                                              AAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTAT                                             >  SRR4302533.1027795/1‑94 (MQ=60)
                                              AAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTAT                                             >  SRR4302533.5377185/1‑94 (MQ=60)
                                              AAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTAT                                             >  SRR4302533.5212589/1‑94 (MQ=60)
                                              AAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTAT                                             >  SRR4302533.1180779/1‑94 (MQ=60)
                                                AGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCA                                           <  SRR4302533.6252514/94‑1 (MQ=60)
                                                AGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCA                                           >  SRR4302533.6246374/1‑94 (MQ=60)
                                                AGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCA                                           >  SRR4302533.5075632/1‑94 (MQ=60)
                                                 GAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCAT                                          >  SRR4302533.1228004/1‑94 (MQ=60)
                                                 GAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCAT                                          <  SRR4302533.2233158/94‑1 (MQ=60)
                                                  AGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATG                                         <  SRR4302533.4169806/94‑1 (MQ=60)
                                                   GGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGG                                        <  SRR4302533.5454692/94‑1 (MQ=60)
                                                    GAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGC                                       <  SRR4302533.1115305/94‑1 (MQ=60)
                                                     AATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCA                                      <  SRR4302533.1626743/94‑1 (MQ=60)
                                                      ATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAG                                     <  SRR4302533.5626588/94‑1 (MQ=60)
                                                       TTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGA                                    <  SRR4302533.3122700/94‑1 (MQ=60)
                                                       TTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGA                                    >  SRR4302533.2076928/1‑94 (MQ=60)
                                                        TCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAA                                   <  SRR4302533.6058942/94‑1 (MQ=60)
                                                         CGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTTAAGCTATCATGGCAGAAT                                  <  SRR4302533.4199760/94‑1 (MQ=60)
                                                         CGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAAT                                  <  SRR4302533.5134629/94‑1 (MQ=60)
                                                         CGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAAT                                  >  SRR4302533.766632/1‑94 (MQ=60)
                                                         CGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAAT                                  >  SRR4302533.2201036/1‑94 (MQ=60)
                                                          GTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATC                                 >  SRR4302533.5723250/1‑94 (MQ=60)
                                                           TATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCA                                >  SRR4302533.3299023/1‑94 (MQ=60)
                                                            ATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAT                               <  SRR4302533.5760334/94‑1 (MQ=60)
                                                            ATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAT                               >  SRR4302533.4414632/1‑94 (MQ=60)
                                                              TAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTT                             >  SRR4302533.2213761/1‑94 (MQ=60)
                                                               AATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTG                            >  SRR4302533.5854057/1‑94 (MQ=60)
                                                               AATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTG                            >  SRR4302533.5360282/1‑94 (MQ=60)
                                                               AATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTG                            <  SRR4302533.683824/94‑1 (MQ=60)
                                                               AATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTG                            <  SRR4302533.5722951/94‑1 (MQ=60)
                                                                ATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGC                           >  SRR4302533.5131810/1‑94 (MQ=60)
                                                                 TGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCA                          <  SRR4302533.3235049/94‑1 (MQ=60)
                                                                  GGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAA                         >  SRR4302533.5880803/1‑94 (MQ=60)
                                                                  GGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGGTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAA                         >  SRR4302533.3507085/1‑94 (MQ=60)
                                                                   GTNATANTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAAC                        <  SRR4302533.1533582/94‑1 (MQ=60)
                                                                   GTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAAT                        >  SRR4302533.3154628/1‑94 (MQ=60)
                                                                   GTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAAC                        >  SRR4302533.356434/1‑94 (MQ=60)
                                                                   GTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAAC                        <  SRR4302533.6269424/94‑1 (MQ=60)
                                                                    TGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACT                       <  SRR4302533.1644897/94‑1 (MQ=60)
                                                                    TGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACT                       <  SRR4302533.2131761/94‑1 (MQ=60)
                                                                       TAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGT                    <  SRR4302533.5066623/94‑1 (MQ=60)
                                                                        AGTCTCACCCAATATTGTAGCGAAAACGCCCGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTT                   >  SRR4302533.2761669/1‑94 (MQ=60)
                                                                        AGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTT                   <  SRR4302533.5586264/94‑1 (MQ=60)
                                                                        AGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTT                   <  SRR4302533.2367722/94‑1 (MQ=60)
                                                                          TCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCC                 <  SRR4302533.3411758/94‑1 (MQ=60)
                                                                           CTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCG                <  SRR4302533.1033753/94‑1 (MQ=60)
                                                                           CTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCG                <  SRR4302533.1552508/94‑1 (MQ=60)
                                                                            TCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGA               >  SRR4302533.862788/1‑94 (MQ=60)
                                                                              ACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATC             >  SRR4302533.2162181/1‑94 (MQ=60)
                                                                                CCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCAT           >  SRR4302533.3424118/1‑94 (MQ=60)
                                                                                 CAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATC          >  SRR4302533.2702497/1‑94 (MQ=60)
                                                                                   ATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAG        >  SRR4302533.5855833/1‑94 (MQ=60)
                                                                                   ATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAG        >  SRR4302533.277461/1‑94 (MQ=60)
                                                                                    TATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGG       <  SRR4302533.5035797/94‑1 (MQ=60)
                                                                                     ATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGT      <  SRR4302533.4720225/94‑1 (MQ=60)
                                                                                     ATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGT      >  SRR4302533.118838/1‑94 (MQ=60)
                                                                                      TTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTT     >  SRR4302533.476280/1‑94 (MQ=60)
                                                                                      TTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTT     <  SRR4302533.3079171/94‑1 (MQ=60)
                                                                                         TAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTA  <  SRR4302533.1579105/94‑1 (MQ=60)
                                                                                            |                                                                                          
GATTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGTA  >  NC_000913/2461614‑2461796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑