Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,465,657	T→C	Y365H (TAT→CAT)	intC →	putative prophage Sf6‑like integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,465,657	0	T	C	99.6%	743.2 / NA	227	Y365H (TAT→CAT)	intC	putative prophage Sf6‑like integrase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (116/110); total (117/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

AACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGTATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAATAG  >  NC_000913/2465567‑2465746

aaCGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCAt                                                                                         >  1:1653750/1‑93 (MQ=255)

    aGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCg                                                                                     >  1:1133445/1‑93 (MQ=255)

    aGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCg                                                                                     >  2:1812242/1‑93 (MQ=255)

      cACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGAt                                                                                   >  1:4805916/1‑93 (MQ=255)

        cGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATaa                                                                                 >  2:1579507/1‑93 (MQ=255)

        cGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATaa                                                                                 >  1:3155953/1‑93 (MQ=255)

         gAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATaaa                                                                                >  1:2691037/1‑93 (MQ=255)

          aaTGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAAc                                                                               >  1:1483117/1‑93 (MQ=255)

          aaTGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAAc                                                                               <  2:4887604/93‑1 (MQ=255)

           aTGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACg                                                                              >  2:2177508/1‑93 (MQ=255)

             ggCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCa                                                                            >  2:5622598/1‑93 (MQ=255)

             ggCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCa                                                                            <  2:879768/93‑1 (MQ=255)

             ggCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCa                                                                            <  1:2406832/93‑1 (MQ=255)

               ccTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCaga                                                                          <  1:917690/93‑1 (MQ=255)

               ccTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCaga                                                                          <  1:2112567/93‑1 (MQ=255)

                 tgctgACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAgaga                                                                        >  1:5986101/1‑93 (MQ=255)

                 tgctgACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAgaga                                                                        >  2:5811154/1‑93 (MQ=255)

                 tgctgACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAgaga                                                                        >  2:4742540/1‑93 (MQ=255)

                  gctgACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGaa                                                                       >  2:3293383/1‑93 (MQ=255)

                   ctgACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGaaa                                                                      <  2:172234/93‑1 (MQ=255)

                    tgACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAat                                                                     >  2:3487108/1‑93 (MQ=255)

                    tgACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAat                                                                     <  2:5413241/93‑1 (MQ=255)

                     gACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAatg                                                                    <  2:364382/93‑1 (MQ=255)

                     gACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAatg                                                                    <  1:2015625/93‑1 (MQ=255)

                     gACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAatg                                                                    >  2:1470296/1‑93 (MQ=255)

                     gACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTAAAACCATGCTCAGCATCTCGATAAACGCAGAGAAatg                                                                    >  1:3694951/1‑93 (MQ=255)

                      aCGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAatga                                                                   <  1:2295638/93‑1 (MQ=255)

                       cgttATTTAAGTGCAACTGTCTCATGCAAACGGCGGATCTGTGCGTGGGATTTACACCCATGCTCAGCATCTCGATAAACGCAGAGAAatgat                                                                  <  2:4417729/90‑1 (MQ=255)

                       cGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAatgat                                                                  >  2:5029280/1‑93 (MQ=255)

                        gCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAatgatg                                                                 <  1:3856030/93‑1 (MQ=255)

                        gCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAatgatg                                                                 <  1:4099231/93‑1 (MQ=255)

                         cTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGc                                                                >  1:1143424/1‑93 (MQ=255)

                         cTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGc                                                                <  2:3548207/93‑1 (MQ=255)

                          tATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCa                                                               >  2:659433/1‑93 (MQ=255)

                          tATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCa                                                               >  1:4997827/1‑93 (MQ=255)

                          tATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGAGAAACGCAGAGAAATGATGCa                                                               >  1:4203613/1‑93 (MQ=255)

                           aTTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCaa                                                              >  2:4230932/1‑93 (MQ=255)

                            ttGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTGGATAAACGCAGAGAAATGATGCAAt                                                             <  2:1154085/93‑1 (MQ=255)

                              gAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAAtgg                                                           <  1:5332803/93‑1 (MQ=255)

                                aGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAAtggtg                                                         <  2:854627/93‑1 (MQ=255)

                                aGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAAtggtg                                                         <  1:3010009/93‑1 (MQ=255)

                                aGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAAtggtg                                                         <  1:2177707/93‑1 (MQ=255)

                                  tGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTggg                                                       <  1:2740574/93‑1 (MQ=255)

                                   gCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGc                                                      >  1:3382660/1‑93 (MQ=255)

                                    cAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCg                                                     >  1:5504805/1‑93 (MQ=255)

                                      aCTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGa                                                   <  1:2540457/93‑1 (MQ=255)

                                        tGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACt                                                 <  1:259148/93‑1 (MQ=255)

                                         ggCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATACTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTg                                                <  1:3611843/93‑1 (MQ=255)

                                           cacaTGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGc                                              >  1:1630224/1‑93 (MQ=255)

                                            acaTGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGAGTgggt                                             >  1:4031684/1‑91 (MQ=255)

                                              aTGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGGttt                                           >  2:4396564/1‑92 (MQ=255)

                                               tGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGATTGGCTtga                                          <  2:6130332/93‑1 (MQ=255)

                                               tGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTtgt                                          >  2:6189066/1‑92 (MQ=255)

                                               tGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTtga                                          >  2:3794429/1‑93 (MQ=255)

                                                gCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTtgat                                         <  2:3452033/93‑1 (MQ=255)

                                                 cAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTtgatg                                        <  2:1065241/93‑1 (MQ=255)

                                                 cAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTtgatg                                        >  2:455221/1‑93 (MQ=255)

                                                 cAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTtgatg                                        <  1:4675500/93‑1 (MQ=255)

                                                   aaCGGCGGATGTGTGCGTGGGATTTACAACCATGCTCAGCATCTTGATAAACGCAGAGAAATGATGCAATGGTGGTCAGACTGGCTtgatggg                                      <  2:6260347/93‑3 (MQ=255)

                                                     cggcggATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGaa                                    <  1:846878/93‑1 (MQ=255)

                                                     cggcggATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGaa                                    <  2:1941405/93‑1 (MQ=255)

                                                      ggcggATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGCATGGCTTTGTGAGAAg                                   >  2:2411511/1‑93 (MQ=255)

                                                      ggcggATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAg                                   <  1:482539/93‑1 (MQ=255)

                                                       gcggATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAgg                                  >  2:763438/1‑93 (MQ=255)

                                                       gcggATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAgg                                  <  1:2800123/93‑1 (MQ=255)

                                                          gATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGgggg                               >  2:397588/1‑93 (MQ=255)

                                                          gATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTgg                               <  1:6167160/93‑1 (MQ=255)

                                                            tCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGgggggg                             >  2:4089642/1‑91 (MQ=255)

                                                              tgtgCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTg                           <  2:3683727/93‑1 (MQ=255)

                                                              tgtgCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTg                           <  2:5517317/93‑1 (MQ=255)

                                                                tgCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGGggagggag                         >  1:893561/1‑93 (MQ=255)

                                                                 gCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGc                        <  1:1417631/93‑1 (MQ=255)

                                                                 gCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGc                        <  1:2834793/93‑1 (MQ=255)

                                                                  cGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGGggagggaggg                       >  1:1543569/1‑91 (MQ=255)

                                                                    tGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGGGGGAGCGAc                     >  1:5130686/1‑93 (MQ=255)

                                                                    tGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGAc                     <  2:5638293/93‑1 (MQ=255)

                                                                    tGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGGGGAGTGAGCGAc                     >  1:725795/1‑93 (MQ=255)

                                                                     gggATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGGGTGAGCGAcc                    >  1:1800203/1‑93 (MQ=255)

                                                                     gggATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGGGCGAcc                    >  2:4281704/1‑93 (MQ=255)

                                                                     gggATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGAcc                    <  2:3195551/93‑1 (MQ=255)

                                                                     gggATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGGGGAGGGAGCGAcc                    >  1:5575919/1‑93 (MQ=255)

                                                                      ggATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAAGTGGAGTGAGCGACCt                   >  1:3931311/1‑93 (MQ=255)

                                                                         tttACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGGTCAGTAGGGGGAGGGAGCGTCCttta                >  2:1264160/1‑91 (MQ=255)

                                                                         tttACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGGAGGTGGAGGGAGAGACCTTaa                >  1:5648426/1‑93 (MQ=255)

                                                                         tttACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTaa                <  1:5173108/93‑1 (MQ=255)

                                                                          ttACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTaac               <  1:4250662/93‑1 (MQ=255)

                                                                            aCAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAAGTGGAGTGAGCGACCTTaacaa             >  2:2686815/1‑93 (MQ=255)

                                                                              aaCCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACt           <  2:4105580/93‑1 (MQ=255)

                                                                              aaCCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGAACTTAACAACt           >  2:5256080/1‑93 (MQ=255)

                                                                              aaCCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGGGAGCGACCTTAACAACt           >  1:440194/1‑93 (MQ=255)

                                                                                  aTGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCg       <  1:3291839/93‑1 (MQ=255)

                                                                                    gCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGTCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGaa     <  1:4437164/93‑1 (MQ=255)

                                                                                    gCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGaa     >  1:4982760/1‑93 (MQ=255)

                                                                                    gCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGAACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGaa     <  2:4778607/93‑1 (MQ=255)

                                                                                     cTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAAt    <  2:5639667/93‑1 (MQ=255)

                                                                                     cTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAAt    <  2:5879691/93‑1 (MQ=255)

                                                                                      tCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAATa   <  2:5732520/93‑1 (MQ=255)

                                                                                      tCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAATa   <  1:4890910/93‑1 (MQ=255)

                                                                                       cAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAATaa  >  1:4293263/1‑92 (MQ=255)

                                                                                       cAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAATAg  <  1:3665748/93‑1 (MQ=255)

AACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGTATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAATAG  >  NC_000913/2465567‑2465746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATGAACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGTATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAATAGCACA  >  NC_000913/2465564‑2465750

ATGAACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGC                                                                                               <  SRR4302533.5930993/94‑1 (MQ=60)

ATGAACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGC                                                                                               >  SRR4302533.5810791/1‑94 (MQ=60)

 TGAACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCA                                                                                              <  SRR4302533.5279969/94‑1 (MQ=60)

 TGAACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCA                                                                                              >  SRR4302533.2133974/1‑94 (MQ=60)

  GAACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCAT                                                                                             >  SRR4302533.1663765/1‑94 (MQ=60)

   AACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATC                                                                                            >  SRR4302533.4446269/1‑94 (MQ=60)

   AACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATC                                                                                            <  SRR4302533.4916330/94‑1 (MQ=60)

   AACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATC                                                                                            >  SRR4302533.2297831/1‑94 (MQ=60)

      GAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCG                                                                                         >  SRR4302533.5297595/1‑94 (MQ=60)

      GAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCG                                                                                         >  SRR4302533.1140184/1‑94 (MQ=60)

        GCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGAT                                                                                       >  SRR4302533.4835017/1‑94 (MQ=60)

         GACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATA                                                                                      <  SRR4302533.4294841/94‑1 (MQ=60)

          ACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAA                                                                                     >  SRR4302533.3175355/1‑94 (MQ=60)

           CGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAA                                                                                    >  SRR4302533.2707533/1‑94 (MQ=60)

           CGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAA                                                                                    >  SRR4302533.5598925/1‑94 (MQ=60)

            GAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAAC                                                                                   >  SRR4302533.5014465/1‑94 (MQ=60)

            GAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAAC                                                                                   >  SRR4302533.1492071/1‑94 (MQ=60)

            GAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAAC                                                                                   <  SRR4302533.788384/94‑1 (MQ=60)

                GGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAG                                                                               <  SRR4302533.2421699/94‑1 (MQ=60)

                  CCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAG                                                                             <  SRR4302533.923083/94‑1 (MQ=60)

                  CCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAG                                                                             <  SRR4302533.2125599/94‑1 (MQ=60)

                   CTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGTATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGA                                                                            <  SRR4302533.1661641/94‑1 (MQ=60)

                   CTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGA                                                                            >  SRR4302533.6022352/1‑94 (MQ=60)

                   CTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGA                                                                            >  SRR4302533.3381176/1‑94 (MQ=60)

                    TGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAA                                                                           >  SRR4302533.2677051/1‑94 (MQ=60)

                      CTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAAT                                                                         >  SRR4302533.2310324/1‑94 (MQ=60)

                       TGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTAAAACCATGCTCAGCATCTCGATAAACGCAGAGAAATG                                                                        >  SRR4302533.3717645/1‑94 (MQ=60)

                        GACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGA                                                                       >  SRR4302533.3327894/1‑94 (MQ=60)

                        GACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGA                                                                       >  SRR4302533.872052/1‑94 (MQ=60)

                        GACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGA                                                                       <  SRR4302533.2028042/94‑1 (MQ=60)

                         ACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGAT                                                                      <  SRR4302533.2309855/94‑1 (MQ=60)

                          CGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATG                                                                     >  SRR4302533.1527591/1‑94 (MQ=60)

                          CGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATG                                                                     >  SRR4302533.5061483/1‑94 (MQ=60)

                          CGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATG                                                                     <  SRR4302533.3035599/94‑1 (MQ=60)

                          CGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATG                                                                     >  SRR4302533.2845455/1‑94 (MQ=60)

                           GCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGC                                                                    <  SRR4302533.4124181/94‑1 (MQ=60)

                           GCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGC                                                                    >  SRR4302533.4117386/1‑94 (MQ=60)

                           GCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGC                                                                    <  SRR4302533.3879575/94‑1 (MQ=60)

                           GCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGC                                                                    >  SRR4302533.1150243/1‑94 (MQ=60)

                            CTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCA                                                                   >  SRR4302533.1161650/1‑94 (MQ=60)

                            CTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCA                                                                   >  SRR4302533.5028126/1‑94 (MQ=60)

                            CTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCA                                                                   <  SRR4302533.4389676/94‑1 (MQ=60)

                            CTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGAGAAACGCAGAGAAATGATGCA                                                                   >  SRR4302533.4229042/1‑94 (MQ=60)

                              ATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAAT                                                                 >  SRR4302533.4564236/1‑94 (MQ=60)

                              ATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAAT                                                                 <  SRR4302533.781141/94‑1 (MQ=60)

                              ATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAAT                                                                 >  SRR4302533.5185013/1‑94 (MQ=60)

                               TTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATG                                                                <  SRR4302533.5998517/94‑1 (MQ=60)

                                TGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGG                                                               <  SRR4302533.4198495/94‑1 (MQ=60)

                                 GAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGT                                                              <  SRR4302533.5364764/94‑1 (MQ=60)

                                  AAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTG                                                             <  SRR4302533.4802661/94‑1 (MQ=60)

                                   AGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGG                                                            <  SRR4302533.3028426/94‑1 (MQ=60)

                                   AGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGG                                                            <  SRR4302533.2191132/94‑1 (MQ=60)

                                   AGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGG                                                            <  SRR4302533.3658187/94‑1 (MQ=60)

                                     TGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGC                                                          >  SRR4302533.3403540/1‑94 (MQ=60)

                                     TGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGC                                                          <  SRR4302533.2757379/94‑1 (MQ=60)

                                      GCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCG                                                         >  SRR4302533.5538021/1‑94 (MQ=60)

                                      GCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCG                                                         >  SRR4302533.3884571/1‑94 (MQ=60)

                                       CAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGG                                                        >  SRR4302533.3736239/1‑94 (MQ=60)

                                       CAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGG                                                        >  SRR4302533.2666899/1‑94 (MQ=60)

                                         ACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGAC                                                      <  SRR4302533.2556095/94‑1 (MQ=60)

                                          CTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACT                                                     <  SRR4302533.1637092/94‑1 (MQ=60)

                                           TGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTG                                                    <  SRR4302533.260723/94‑1 (MQ=60)

                                            GGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATACTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGG                                                   <  SRR4302533.3634071/94‑1 (MQ=60)

                                             GCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGC                                                  >  SRR4302533.1640100/1‑94 (MQ=60)

                                              CACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGAGTGGGT                                                 >  SRR4302533.4056225/1‑94 (MQ=60)

                                              CACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCT                                                 <  SRR4302533.6330478/94‑1 (MQ=60)

                                               ACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTT                                                >  SRR4302533.1528514/1‑94 (MQ=60)

                                               ACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTT                                                >  SRR4302533.5619454/1‑94 (MQ=60)

                                                CATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTG                                               >  SRR4302533.1898656/1‑94 (MQ=60)

                                                 ATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGT                                              >  SRR4302533.1482630/1‑94 (MQ=60)

                                                    CAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGG                                           >  SRR4302533.484361/1‑94 (MQ=60)

                                                    CAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGA                                           <  SRR4302533.4703834/94‑1 (MQ=60)

                                                    CAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGA                                           <  SRR4302533.6186731/94‑1 (MQ=60)

                                                      AACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGGTGAGA                                         >  SRR4302533.5814489/1‑94 (MQ=60)

                                                        CGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGgggag                                       >  SRR4302533.1297953/1‑89 (MQ=60)

                                                        CGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAG                                       <  SRR4302533.851836/94‑1 (MQ=60)

                                                         GGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCACTGGTGGGCGGGCTGGCTTGATTAGAAGG                                      >  SRR4302533.2604832/1‑94 (MQ=60)

                                                         GGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGG                                      <  SRR4302533.485421/94‑1 (MQ=60)

                                                          GCGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGT                                     <  SRR4302533.2817345/94‑1 (MQ=60)

                                                           CGGATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTG                                    <  SRR4302533.4981084/94‑1 (MQ=60)

                                                             GATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGA                                  <  SRR4302533.6204230/94‑1 (MQ=60)

                                                             GATCTGTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGA                                  <  SRR4302533.4723140/94‑1 (MQ=60)

                                                                  GTGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGGGGAGGGAG                             >  SRR4302533.898813/1‑94 (MQ=60)

                                                                   TGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAagtggggggagg                            >  SRR4302533.9085/1‑82 (MQ=60)

                                                                   TGCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGG                            >  SRR4302533.2196514/1‑94 (MQ=60)

                                                                    GCGTGTGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCG                           <  SRR4302533.2837170/94‑1 (MQ=60)

                                                                    GCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCG                           <  SRR4302533.2852240/94‑1 (MQ=60)

                                                                    GCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCG                           <  SRR4302533.4269764/94‑1 (MQ=60)

                                                                    GCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCG                           <  SRR4302533.1426208/94‑1 (MQ=60)

                                                                    GCGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGGGGAGGGAGGG                           >  SRR4302533.1552867/1‑94 (MQ=60)

                                                                     CGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGGGGAGGGAGCGA                          >  SRR4302533.5332720/1‑94 (MQ=60)

                                                                     CGTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAAGTGGAGTGAGCGA                          >  SRR4302533.2750053/1‑94 (MQ=60)

                                                                      GTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGgggagggggggac                         >  SRR4302533.1798783/1‑81 (MQ=60)

                                                                      GTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGGGGGAGCGAC                         >  SRR4302533.5161679/1‑94 (MQ=60)

                                                                      GTGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGGGGAGTGAGCGAC                         >  SRR4302533.730083/1‑94 (MQ=60)

                                                                       TGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGGGTGAGCGACC                        >  SRR4302533.1811156/1‑94 (MQ=60)

                                                                       TGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGGGAGAGACC                        >  SRR4302533.3992711/1‑94 (MQ=60)

                                                                       TGGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGGGGAGGGAGCGACC                        >  SRR4302533.5609616/1‑94 (MQ=60)

                                                                        GGGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAAGTGGAGTGAGCGACCT                       >  SRR4302533.3955311/1‑94 (MQ=60)

                                                                         GGATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTT                      >  SRR4302533.5462241/1‑94 (MQ=60)

                                                                           ATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGGAGGTGGAGGGAGAGACCTTAA                    >  SRR4302533.5682574/1‑94 (MQ=60)

                                                                           ATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAA                    >  SRR4302533.3630608/1‑94 (MQ=60)

                                                                           ATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAA                    >  SRR4302533.3698610/1‑94 (MQ=60)

                                                                           ATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAA                    >  SRR4302533.3700893/1‑94 (MQ=60)

                                                                           ATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAA                    <  SRR4302533.3024153/94‑1 (MQ=60)

                                                                           ATTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAAGGGGAGTGAGCGACCTTAA                    >  SRR4302533.1702164/1‑94 (MQ=60)

                                                                            TTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAAC                   <  SRR4302533.5204321/94‑1 (MQ=60)

                                                                            TTTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAAC                   <  SRR4302533.324078/94‑1 (MQ=60)

                                                                             TTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACA                  <  SRR4302533.5018070/94‑1 (MQ=60)

                                                                             TTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACA                  <  SRR4302533.4276308/94‑1 (MQ=60)

                                                                             TTACAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACA                  <  SRR4302533.4378020/94‑1 (MQ=60)

                                                                             TTACAACCATACTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAAGTGGAGTGAGCGACCTTAACA                  >  SRR4302533.2801581/1‑94 (MQ=60)

                                                                                CAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACT               <  SRR4302533.2099743/94‑1 (MQ=60)

                                                                                CAACCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGGGAGCGACCTTAACAACT               >  SRR4302533.442820/1‑94 (MQ=60)

                                                                                   CCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGGGAGCGACCTTAAAAACTATC            >  SRR4302533.3620319/1‑94 (MQ=60)

                                                                                   CCATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTCATGAGAAGGGGGGGTGAGCGACCTTAACAACTATC            >  SRR4302533.412357/1‑94 (MQ=60)

                                                                                    CATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGGGTGAGCGGCCTTAACAACTATCG           >  SRR4302533.1149307/1‑94 (MQ=60)

                                                                                     ATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGA          >  SRR4302533.2436877/1‑94 (MQ=60)

                                                                                     ATGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGA          <  SRR4302533.3312159/94‑1 (MQ=60)

                                                                                      TGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAA         >  SRR4302533.5012972/1‑94 (MQ=60)

                                                                                      TGCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAA         <  SRR4302533.1834150/94‑1 (MQ=60)

                                                                                       GCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGTCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAAT        <  SRR4302533.4463986/94‑1 (MQ=60)

                                                                                       GCTCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAAT        <  SRR4302533.2133457/94‑1 (MQ=60)

                                                                                         TCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAATAG      <  SRR4302533.4920542/94‑1 (MQ=60)

                                                                                         TCAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAATAA      >  SRR4302533.4319084/1‑94 (MQ=60)

                                                                                          CAGCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAATAGC     <  SRR4302533.3688281/94‑1 (MQ=60)

                                                                                            GCATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAATAGCAC   <  SRR4302533.4450682/94‑1 (MQ=60)

                                                                                             CATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAATAGCACA  <  SRR4302533.2344990/94‑1 (MQ=60)

ATGAACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTGGCACATGCAAACGGCGGATCTGTGCGTGGGATTTACAACCATGCTCAGTATCTCGATAAACGCAGAGAAATGATGCAATGGTGGGCGGACTGGCTTGATGAGAAGGTGGAGTGAGCGACCTTAACAACTATCGAATAGCACA  >  NC_000913/2465564‑2465750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`